AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1166040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1166040 MtrunA17_Chr7g0269081 95.779 308 9 3 1 306 1 306 0.0 586
Msa1166040 MtrunA17_Chr4g0067291 34.796 319 146 14 19 306 71 358 1.14e-33 126
Msa1166040 MtrunA17_Chr1g0208301 67.606 71 23 0 16 86 7 77 6.32e-26 105
Msa1166040 MtrunA17_Chr2g0287751 67.143 70 23 0 17 86 5 74 5.42e-24 101
Msa1166040 MtrunA17_Chr4g0065651 65.714 70 24 0 17 86 5 74 5.28e-23 99.0
Msa1166040 MtrunA17_Chr0c22g0493881 65.714 70 24 0 17 86 5 74 6.31e-23 97.4
Msa1166040 MtrunA17_Chr4g0065621 65.714 70 24 0 17 86 5 74 6.11e-22 94.7
Msa1166040 MtrunA17_Chr2g0296001 45.385 130 62 2 2 130 61 182 1.55e-21 93.2
Msa1166040 MtrunA17_Chr1g0192131 53.933 89 33 2 1 88 1 82 1.10e-20 91.3
Msa1166040 MtrunA17_Chr7g0272571 54.286 70 32 0 22 91 33 102 1.15e-19 88.2
Msa1166040 MtrunA17_Chr1g0191671 57.143 70 30 0 17 86 5 74 3.01e-19 88.2
Msa1166040 MtrunA17_Chr3g0111541 48.611 72 37 0 17 88 7 78 2.45e-15 73.6
Msa1166040 MtrunA17_Chr2g0287741 61.905 63 24 0 24 86 17 79 6.18e-15 75.1
Msa1166040 MtrunA17_Chr1g0160201 43.478 69 39 0 22 90 25 93 4.87e-14 68.6
Msa1166040 MtrunA17_Chr4g0036931 44.444 72 39 1 20 90 26 97 5.53e-14 68.6
Msa1166040 MtrunA17_Chr3g0124411 47.887 71 37 0 20 90 28 98 8.96e-14 67.8
Msa1166040 MtrunA17_Chr5g0407821 42.424 66 38 0 22 87 23 88 3.65e-13 65.9
Msa1166040 MtrunA17_Chr8g0382721 47.692 65 34 0 26 90 33 97 8.86e-13 65.1
Msa1166040 MtrunA17_Chr7g0218981 40.000 70 42 0 19 88 1 70 1.95e-12 63.5
Msa1166040 MtrunA17_Chr3g0115421 33.010 103 56 2 26 115 30 132 7.95e-12 62.4
Msa1166040 MtrunA17_Chr3g0145621 37.500 72 44 1 20 90 24 95 1.70e-11 61.6
Msa1166040 MtrunA17_Chr1g0198341 40.260 77 45 1 19 94 1 77 1.93e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1166040 AT3G06130.2 65.714 70 24 0 17 86 5 74 1.20e-23 99.4
Msa1166040 AT3G06130.1 65.714 70 24 0 17 86 5 74 1.29e-23 100
Msa1166040 AT5G27690.1 59.155 71 29 0 17 87 25 95 3.71e-23 98.2
Msa1166040 AT5G19090.2 64.286 70 25 0 17 86 5 74 1.76e-22 97.4
Msa1166040 AT5G19090.3 64.286 70 25 0 17 86 5 74 1.76e-22 97.4
Msa1166040 AT5G19090.5 64.286 70 25 0 17 86 5 74 2.30e-22 97.8
Msa1166040 AT5G19090.4 64.286 70 25 0 17 86 5 74 2.30e-22 97.8
Msa1166040 AT5G19090.1 64.286 70 25 0 17 86 5 74 2.30e-22 97.8
Msa1166040 AT1G23000.1 45.455 99 46 1 16 106 7 105 5.67e-21 92.0
Msa1166040 AT1G23000.2 45.455 99 46 1 16 106 67 165 1.25e-20 91.7
Msa1166040 AT1G56210.1 54.286 70 32 0 21 90 39 108 2.25e-19 87.8
Msa1166040 AT3G05220.1 55.714 70 31 0 17 86 5 74 3.01e-18 85.5
Msa1166040 AT5G37860.1 48.611 72 37 0 22 93 10 81 2.38e-15 75.1
Msa1166040 AT1G71050.1 42.029 69 40 0 22 90 28 96 9.53e-13 65.5
Msa1166040 AT1G06330.1 45.902 61 33 0 26 86 6 66 4.88e-12 63.2
Msa1166040 AT4G38580.1 40.278 72 42 1 20 90 24 95 1.82e-11 61.6
Msa1166040 AT4G38580.2 40.278 72 42 1 20 90 24 95 1.82e-11 61.6
Msa1166040 AT3G56240.3 43.939 66 37 0 22 87 3 68 2.01e-11 60.8
Msa1166040 AT3G56240.1 43.939 66 37 0 22 87 3 68 2.01e-11 60.8
Msa1166040 AT3G56891.1 43.548 62 35 0 26 87 20 81 3.18e-11 61.2
Msa1166040 AT3G56891.2 43.103 58 33 0 30 87 1 58 3.64e-11 60.8

Find 104 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCACAAGGTTACAGTTAC+TGG 0.264090 7_4:-58055933 Msa1166040:CDS
TGAGAGGGTGGAGGAGGTGG+AGG 0.275287 7_4:+58055276 None:intergenic
GCTTCATTAACTCCAAATAA+TGG 0.285010 7_4:-58055573 Msa1166040:CDS
GCTTAGTGGAGTGGAAAATA+AGG 0.302835 7_4:+58056951 None:intergenic
AAGAGTCAAGAAAATTCTTC+AGG 0.319616 7_4:-58056658 Msa1166040:CDS
TGAAACTGAAAATATTAATA+AGG 0.330846 7_4:-58055796 Msa1166040:CDS
ACAGAACAGAAATTGATTCT+TGG 0.330890 7_4:-58055956 Msa1166040:CDS
TAGTACATATGTGGTGGATA+TGG 0.331565 7_4:+58055429 None:intergenic
GTTAATTAGTGCTTAATGTA+AGG 0.337945 7_4:-58055132 Msa1166040:three_prime_UTR
TGTATAGCTTTGTCACATAA+TGG 0.351866 7_4:+58055194 None:intergenic
TGTTGATCAGCAGCTGGTTT+GGG 0.352102 7_4:+58056889 None:intergenic
ATTAGAAACACTCAAGTATC+AGG 0.357551 7_4:-58056854 Msa1166040:intron
CAGCCATTTGAAATGAAGTT+TGG 0.358581 7_4:+58056914 None:intergenic
ATCAAGAAGCTTTCAAGATC+AGG 0.368275 7_4:-58055888 Msa1166040:CDS
TTGTTGATCAGCAGCTGGTT+TGG 0.374527 7_4:+58056888 None:intergenic
GGATGAGAGGGTGGAGGAGG+TGG 0.374539 7_4:+58055273 None:intergenic
ATACTTGAGTGTTTCTAATC+CGG 0.381184 7_4:+58056858 None:intergenic
GCAGAGCATTACAATGCTAT+TGG 0.387344 7_4:+58054752 None:intergenic
AGCACTTCTTCTTATTATGT+TGG 0.397233 7_4:-58055339 Msa1166040:CDS
TCTAAGTGGGGTGTGTTTGT+TGG 0.404477 7_4:+58056989 None:intergenic
CAACCAAACTTCATTTCAAA+TGG 0.404986 7_4:-58056917 Msa1166040:exon
GTGTAGTTTGCTTGCTGGTA+TGG 0.419290 7_4:-58054871 Msa1166040:three_prime_UTR
TGCTTAATGTAAGGGAAAGT+GGG 0.420370 7_4:-58055123 Msa1166040:three_prime_UTR
GCTACTGCTGGAGGAGGGTG+TGG 0.422467 7_4:+58055405 None:intergenic
TAAGAAGAAGTGCTAGAAAC+AGG 0.428358 7_4:+58055348 None:intergenic
ATATGGTGTAGTTTGCTTGC+TGG 0.428624 7_4:-58054876 Msa1166040:three_prime_UTR
CTCACACCCTTTAAAAGCAT+TGG 0.430712 7_4:-58057017 Msa1166040:five_prime_UTR
AAAATTCTTCAGGGAATTGA+AGG 0.432486 7_4:-58056648 Msa1166040:intron
TGATGACCATAGCTGTTGAT+TGG 0.433870 7_4:+58055252 None:intergenic
GTGCTTAATGTAAGGGAAAG+TGG 0.434606 7_4:-58055124 Msa1166040:three_prime_UTR
TTAGCATGCATGGGCATGAC+AGG 0.444727 7_4:+58055312 None:intergenic
GGTGGTGCTACTGCTGGAGG+AGG 0.445055 7_4:+58055399 None:intergenic
CCAGCAGTAGCACCACCCTA+TGG 0.447198 7_4:-58055393 Msa1166040:CDS
ATTGGATGAGAGGGTGGAGG+AGG 0.450211 7_4:+58055270 None:intergenic
GCATGCTGCTGAGGAAGTAC+TGG 0.452781 7_4:+58055459 None:intergenic
GATCACAAAGAAGGTCATAA+TGG 0.457322 7_4:-58055669 Msa1166040:CDS
TTAATTAGTGCTTAATGTAA+GGG 0.467475 7_4:-58055131 Msa1166040:three_prime_UTR
GGAGGCATAATAATTTCCTT+AGG 0.468365 7_4:+58055480 None:intergenic
AGTACATATGTGGTGGATAT+GGG 0.469995 7_4:+58055430 None:intergenic
ACGAAGAAAGCTTGTCTAAG+TGG 0.471666 7_4:+58056975 None:intergenic
TTGGTACGTAGCTAGCTTAG+TGG 0.480383 7_4:+58056937 None:intergenic
TACTTGAGTGTTTCTAATCC+GGG 0.491731 7_4:+58056859 None:intergenic
CATAATAATTTCCTTAGGAA+TGG 0.491755 7_4:+58055485 None:intergenic
CTTAGTGGAGTGGAAAATAA+GGG 0.493762 7_4:+58056952 None:intergenic
GGAACTTGTTGATCAGCAGC+TGG 0.496704 7_4:+58056883 None:intergenic
TACTATACTATACATGTACA+TGG 0.497297 7_4:-58057044 Msa1166040:five_prime_UTR
CATTTGAAATGAAGTTTGGT+TGG 0.501265 7_4:+58056918 None:intergenic
TGTATAGATGATGCAATTGC+AGG 0.502278 7_4:-58055714 Msa1166040:CDS
AGTTTGCTTGCTGGTATGGT+GGG 0.503457 7_4:-58054867 Msa1166040:three_prime_UTR
CCATAGGGTGGTGCTACTGC+TGG 0.507340 7_4:+58055393 None:intergenic
TCTAGAGTAATTAGCATGCA+TGG 0.510452 7_4:+58055302 None:intergenic
AAATATTAATAAGGAGATCA+AGG 0.511134 7_4:-58055787 Msa1166040:CDS
CGAAGAAAGCTTGTCTAAGT+GGG 0.515764 7_4:+58056976 None:intergenic
TAGTTTGCTTGCTGGTATGG+TGG 0.517121 7_4:-58054868 Msa1166040:three_prime_UTR
CAGGGAAATCAGTAGAACTT+TGG 0.517951 7_4:-58055869 Msa1166040:CDS
GAAGATTCAGATCACAAAGA+AGG 0.518960 7_4:-58055678 Msa1166040:CDS
GAGAGGGTGGAGGAGGTGGA+GGG 0.528150 7_4:+58055277 None:intergenic
TAATGCAGATGTTGCAAAGT+CGG 0.537492 7_4:+58054965 None:intergenic
TCAAGAAGCTTTCAAGATCA+GGG 0.538607 7_4:-58055887 Msa1166040:CDS
GATGCAATTGCAGGTGATGA+TGG 0.539060 7_4:-58055705 Msa1166040:CDS
TAGGGTGGTGCTACTGCTGG+AGG 0.544543 7_4:+58055396 None:intergenic
GTGGTGCTACTGCTGGAGGA+GGG 0.547041 7_4:+58055400 None:intergenic
CAGCAGTAGCACCACCCTAT+GGG 0.554674 7_4:-58055392 Msa1166040:CDS
TTGATTGGATGAGAGGGTGG+AGG 0.558090 7_4:+58055267 None:intergenic
GATAATAACAATAAAAGTGA+GGG 0.562957 7_4:-58055645 Msa1166040:CDS
TGTGTTATAGCTTAACCCAT+AGG 0.565506 7_4:+58055377 None:intergenic
GTGTTATAGCTTAACCCATA+GGG 0.565951 7_4:+58055378 None:intergenic
AACATCTGCATTAGAAGTTG+CGG 0.568622 7_4:-58054955 Msa1166040:three_prime_UTR
AGAGTCAAGAAAATTCTTCA+GGG 0.568811 7_4:-58056657 Msa1166040:CDS
AAAAGTGAATGCCATTCCTA+AGG 0.569713 7_4:-58055496 Msa1166040:CDS
TTAACTCCAAATAATGGAGG+AGG 0.576698 7_4:-58055567 Msa1166040:CDS
AATTGATTCTTGGCAGCACA+AGG 0.578132 7_4:-58055946 Msa1166040:CDS
GTTGGTGAAAGTAGTCATGA+AGG 0.580432 7_4:-58055738 Msa1166040:CDS
CTAGAGTAATTAGCATGCAT+GGG 0.582346 7_4:+58055303 None:intergenic
GGGTTATAAGCATGCTGCTG+AGG 0.582921 7_4:+58055450 None:intergenic
GCTGATCAACAAGTTCCTCC+CGG 0.583544 7_4:-58056877 Msa1166040:CDS
GCTTAATGTAAGGGAAAGTG+GGG 0.583691 7_4:-58055122 Msa1166040:three_prime_UTR
TCTCATCCAATCAACAGCTA+TGG 0.586584 7_4:-58055258 Msa1166040:CDS
TAGCATGCATGGGCATGACA+GGG 0.586920 7_4:+58055313 None:intergenic
AATGATCATGAAGAATATGA+AGG 0.589711 7_4:-58055228 Msa1166040:CDS
TGATAATAACAATAAAAGTG+AGG 0.591536 7_4:-58055646 Msa1166040:CDS
GATCATGAAGAATATGAAGG+TGG 0.598780 7_4:-58055225 Msa1166040:CDS
CTGTTGATTGGATGAGAGGG+TGG 0.603779 7_4:+58055264 None:intergenic
AATAACAATAAAAGTGAGGG+TGG 0.610406 7_4:-58055642 Msa1166040:CDS
AAAAGACAATAATTGTGAGA+CGG 0.612655 7_4:-58057092 Msa1166040:five_prime_UTR
GGGTGTGGATAGTACATATG+TGG 0.614125 7_4:+58055420 None:intergenic
TGCTGCTGAGGAAGTACTGG+AGG 0.614651 7_4:+58055462 None:intergenic
AAAGTCTTGATCCACTGCGA+CGG 0.628481 7_4:-58056690 Msa1166040:CDS
AAGAACAGTACTGAACAAGT+TGG 0.630407 7_4:-58055756 Msa1166040:CDS
TGTGGATAGTACATATGTGG+TGG 0.633196 7_4:+58055423 None:intergenic
ACGTAGCTAGCTTAGTGGAG+TGG 0.638739 7_4:+58056942 None:intergenic
CTTTACACATAAGATAGAGA+AGG 0.643549 7_4:+58054703 None:intergenic
ATAGCTGTTGATTGGATGAG+AGG 0.644634 7_4:+58055260 None:intergenic
AGCATGCATGGGCATGACAG+GGG 0.652187 7_4:+58055314 None:intergenic
TAGCTGTTGATTGGATGAGA+GGG 0.658334 7_4:+58055261 None:intergenic
ACTATACTATACATGTACAT+GGG 0.661840 7_4:-58057043 Msa1166040:five_prime_UTR
TCATTAACTCCAAATAATGG+AGG 0.678633 7_4:-58055570 Msa1166040:CDS
TTATAGCTTAACCCATAGGG+TGG 0.684519 7_4:+58055381 None:intergenic
GAAGAAAGCTTGTCTAAGTG+GGG 0.685656 7_4:+58056977 None:intergenic
TTGAGTGTTTCTAATCCGGG+AGG 0.702857 7_4:+58056862 None:intergenic
TGTGACAAAGCTATACAATG+TGG 0.703248 7_4:-58055188 Msa1166040:exon
GGTTACAGTTACTGGCAACG+TGG 0.704095 7_4:-58055925 Msa1166040:CDS
CTTAATGTAAGGGAAAGTGG+GGG 0.705125 7_4:-58055121 Msa1166040:three_prime_UTR
GACAAAGCTATACAATGTGG+AGG 0.713576 7_4:-58055185 Msa1166040:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAATAAATGCTATAT+TGG - chr7_4:58054696-58054715 Msa1166040:three_prime_UTR 10.0%
!! TGTAATAGAAAAAAAAAATA+TGG - chr7_4:58056961-58056980 Msa1166040:five_prime_UTR 10.0%
!!! TATTTTGAGAAAAAGAAAAA+AGG - chr7_4:58054736-58054755 Msa1166040:three_prime_UTR 15.0%
!! TAATAGCTTATTAAACATAT+TGG - chr7_4:58055505-58055524 Msa1166040:CDS 15.0%
!! TATATTTGATTAAAGTCTAA+CGG - chr7_4:58055837-58055856 Msa1166040:CDS 15.0%
!! AAAAAATCTTCAAAATCAAA+AGG - chr7_4:58056035-58056054 Msa1166040:intron 15.0%
!! TGAAACTGAAAATATTAATA+AGG - chr7_4:58056058-58056077 Msa1166040:intron 15.0%
!!! AAAAAACTATACTTTTTAAC+AGG + chr7_4:58057064-58057083 None:intergenic 15.0%
!!! TGTTAAAAAGTATAGTTTTT+TGG - chr7_4:58057063-58057082 Msa1166040:five_prime_UTR 15.0%
!!! GTTAAAAAGTATAGTTTTTT+GGG - chr7_4:58057064-58057083 Msa1166040:five_prime_UTR 15.0%
!!! TATATCATAGTTCTAAATTG+CGG + chr7_4:58055394-58055413 None:intergenic 20.0%
!! CTGATAAAATAAATACCATT+AGG + chr7_4:58055445-58055464 None:intergenic 20.0%
!! AAATATTAATAAGGAGATCA+AGG - chr7_4:58056067-58056086 Msa1166040:intron 20.0%
!! TGATAATAACAATAAAAGTG+AGG - chr7_4:58056208-58056227 Msa1166040:intron 20.0%
!! GATAATAACAATAAAAGTGA+GGG - chr7_4:58056209-58056228 Msa1166040:intron 20.0%
!! AAGAAAAAGAAAAACAAAAG+TGG - chr7_4:58056254-58056273 Msa1166040:intron 20.0%
!! TTAATTAGTGCTTAATGTAA+GGG - chr7_4:58056723-58056742 Msa1166040:intron 20.0%
!! AGATCTAGATTAAAAAAATG+TGG - chr7_4:58056923-58056942 Msa1166040:five_prime_UTR 20.0%
!! GATCTAGATTAAAAAAATGT+GGG - chr7_4:58056924-58056943 Msa1166040:five_prime_UTR 20.0%
!! ATCTAGATTAAAAAAATGTG+GGG - chr7_4:58056925-58056944 Msa1166040:five_prime_UTR 20.0%
!! ATACAAAGAAGAAGAAAAAA+AGG + chr7_4:58057038-58057057 None:intergenic 20.0%
! AAAAGACAATAATTGTGAGA+CGG - chr7_4:58054762-58054781 Msa1166040:three_prime_UTR 25.0%
! TACTATACTATACATGTACA+TGG - chr7_4:58054810-58054829 Msa1166040:three_prime_UTR 25.0%
! ACTATACTATACATGTACAT+GGG - chr7_4:58054811-58054830 Msa1166040:three_prime_UTR 25.0%
!! ATATTTTTCTGAGTACCTAA+TGG - chr7_4:58055427-58055446 Msa1166040:CDS 25.0%
! CATGTAATTAACTAAATTCC+AGG + chr7_4:58055578-58055597 None:intergenic 25.0%
!!! GATTTTTTATCTTTCTTCTC+AGG + chr7_4:58056023-58056042 None:intergenic 25.0%
! CATAATAATTTCCTTAGGAA+TGG + chr7_4:58056372-58056391 None:intergenic 25.0%
! AATGATCATGAAGAATATGA+AGG - chr7_4:58056626-58056645 Msa1166040:intron 25.0%
! GTTAATTAGTGCTTAATGTA+AGG - chr7_4:58056722-58056741 Msa1166040:intron 25.0%
!! AAATCGTCTTTTTACAAACT+AGG + chr7_4:58056801-58056820 None:intergenic 25.0%
!! TAGTTTGTAAAAAGACGATT+TGG - chr7_4:58056800-58056819 Msa1166040:intron 25.0%
!!! TTTTTGGGTTTGCTAAAAAA+AGG - chr7_4:58057079-58057098 Msa1166040:five_prime_UTR 25.0%
! GTTTTGCCAATGCTTTTAAA+GGG + chr7_4:58054846-58054865 None:intergenic 30.0%
! CATTTGAAATGAAGTTTGGT+TGG + chr7_4:58054939-58054958 None:intergenic 30.0%
CAACCAAACTTCATTTCAAA+TGG - chr7_4:58054937-58054956 Msa1166040:three_prime_UTR 30.0%
! ATACTTGAGTGTTTCTAATC+CGG + chr7_4:58054999-58055018 None:intergenic 30.0%
ATTAGAAACACTCAAGTATC+AGG - chr7_4:58055000-58055019 Msa1166040:three_prime_UTR 30.0%
AAGAGTCAAGAAAATTCTTC+AGG - chr7_4:58055196-58055215 Msa1166040:exon 30.0%
AGAGTCAAGAAAATTCTTCA+GGG - chr7_4:58055197-58055216 Msa1166040:exon 30.0%
AAAATTCTTCAGGGAATTGA+AGG - chr7_4:58055206-58055225 Msa1166040:CDS 30.0%
AATGAAGACAAATACAACTG+AGG - chr7_4:58055308-58055327 Msa1166040:CDS 30.0%
CAATTATGGCTGCAATATAA+AGG + chr7_4:58055361-58055380 None:intergenic 30.0%
TTATATTGCAGCCATAATTG+CGG - chr7_4:58055361-58055380 Msa1166040:CDS 30.0%
TTAGAAGACTAACACTTAAG+TGG + chr7_4:58055488-58055507 None:intergenic 30.0%
ATTGACATGATCAATAAACC+TGG - chr7_4:58055557-58055576 Msa1166040:CDS 30.0%
TATATCCGTGACAATCATAA+CGG + chr7_4:58055869-58055888 None:intergenic 30.0%
TATGATTGTCACGGATATAT+AGG - chr7_4:58055870-58055889 Msa1166040:CDS 30.0%
ATGATTGTCACGGATATATA+GGG - chr7_4:58055871-58055890 Msa1166040:CDS 30.0%
! ACAGAACAGAAATTGATTCT+TGG - chr7_4:58055898-58055917 Msa1166040:CDS 30.0%
! TTTTTATCTTTCTTCTCAGG+AGG + chr7_4:58056020-58056039 None:intergenic 30.0%
AATAACAATAAAAGTGAGGG+TGG - chr7_4:58056212-58056231 Msa1166040:intron 30.0%
GCTTCATTAACTCCAAATAA+TGG - chr7_4:58056281-58056300 Msa1166040:intron 30.0%
TCATTAACTCCAAATAATGG+AGG - chr7_4:58056284-58056303 Msa1166040:intron 30.0%
!!! TTCACTTTTTCAAGTACTTG+TGG + chr7_4:58056347-58056366 None:intergenic 30.0%
AGCACTTCTTCTTATTATGT+TGG - chr7_4:58056515-58056534 Msa1166040:intron 30.0%
!! TGTATAGCTTTGTCACATAA+TGG + chr7_4:58056663-58056682 None:intergenic 30.0%
ATTAAAAAAATGTGGGGACA+AGG - chr7_4:58056931-58056950 Msa1166040:five_prime_UTR 30.0%
CTTTACACATAAGATAGAGA+AGG + chr7_4:58057154-58057173 None:intergenic 30.0%
! GGTTTTGCCAATGCTTTTAA+AGG + chr7_4:58054847-58054866 None:intergenic 35.0%
CTTAGTGGAGTGGAAAATAA+GGG + chr7_4:58054905-58054924 None:intergenic 35.0%
! CAGCCATTTGAAATGAAGTT+TGG + chr7_4:58054943-58054962 None:intergenic 35.0%
! TACTTGAGTGTTTCTAATCC+GGG + chr7_4:58054998-58055017 None:intergenic 35.0%
!! TTAGTAGACTTGTTAGAGCA+AGG + chr7_4:58055053-58055072 None:intergenic 35.0%
AGCAAAGAATAGATTCAGGA+GGG + chr7_4:58055081-58055100 None:intergenic 35.0%
GTTGAGCAAAGAATAGATTC+AGG + chr7_4:58055085-58055104 None:intergenic 35.0%
TTGGTTAAAGAGTAGTGTCT+TGG - chr7_4:58055524-58055543 Msa1166040:CDS 35.0%
ATCCACTTCAGACTTTATCA+TGG - chr7_4:58055651-58055670 Msa1166040:CDS 35.0%
ATCCATGATAAAGTCTGAAG+TGG + chr7_4:58055656-58055675 None:intergenic 35.0%
AATATACGTAAGAGTCGTAC+AGG + chr7_4:58055823-58055842 None:intergenic 35.0%
TGATTGTCACGGATATATAG+GGG - chr7_4:58055872-58055891 Msa1166040:CDS 35.0%
ATCAAGAAGCTTTCAAGATC+AGG - chr7_4:58055966-58055985 Msa1166040:intron 35.0%
TCAAGAAGCTTTCAAGATCA+GGG - chr7_4:58055967-58055986 Msa1166040:intron 35.0%
! AAGAACAGTACTGAACAAGT+TGG - chr7_4:58056098-58056117 Msa1166040:intron 35.0%
TGTATAGATGATGCAATTGC+AGG - chr7_4:58056140-58056159 Msa1166040:intron 35.0%
GAAGATTCAGATCACAAAGA+AGG - chr7_4:58056176-58056195 Msa1166040:intron 35.0%
GATCACAAAGAAGGTCATAA+TGG - chr7_4:58056185-58056204 Msa1166040:intron 35.0%
! TTAACTCCAAATAATGGAGG+AGG - chr7_4:58056287-58056306 Msa1166040:intron 35.0%
! TCTTTTCCTCCTCCATTATT+TGG + chr7_4:58056296-58056315 None:intergenic 35.0%
AAAAGTGAATGCCATTCCTA+AGG - chr7_4:58056358-58056377 Msa1166040:intron 35.0%
! GGAGGCATAATAATTTCCTT+AGG + chr7_4:58056377-58056396 None:intergenic 35.0%
!! AGTACATATGTGGTGGATAT+GGG + chr7_4:58056427-58056446 None:intergenic 35.0%
!! TAGTACATATGTGGTGGATA+TGG + chr7_4:58056428-58056447 None:intergenic 35.0%
GTGTTATAGCTTAACCCATA+GGG + chr7_4:58056479-58056498 None:intergenic 35.0%
TGTGTTATAGCTTAACCCAT+AGG + chr7_4:58056480-58056499 None:intergenic 35.0%
!! TAAGAAGAAGTGCTAGAAAC+AGG + chr7_4:58056509-58056528 None:intergenic 35.0%
CTAGAGTAATTAGCATGCAT+GGG + chr7_4:58056554-58056573 None:intergenic 35.0%
! TCTAGAGTAATTAGCATGCA+TGG + chr7_4:58056555-58056574 None:intergenic 35.0%
GATCATGAAGAATATGAAGG+TGG - chr7_4:58056629-58056648 Msa1166040:intron 35.0%
TGTGACAAAGCTATACAATG+TGG - chr7_4:58056666-58056685 Msa1166040:CDS 35.0%
TGCTTAATGTAAGGGAAAGT+GGG - chr7_4:58056731-58056750 Msa1166040:intron 35.0%
TAATGCAGATGTTGCAAAGT+CGG + chr7_4:58056892-58056911 None:intergenic 35.0%
AACATCTGCATTAGAAGTTG+CGG - chr7_4:58056899-58056918 Msa1166040:CDS 35.0%
!! CAATGCTTTTAAAGGGTGTG+AGG + chr7_4:58054839-58054858 None:intergenic 40.0%
! CTCACACCCTTTAAAAGCAT+TGG - chr7_4:58054837-58054856 Msa1166040:three_prime_UTR 40.0%
GAAGAAAGCTTGTCTAAGTG+GGG + chr7_4:58054880-58054899 None:intergenic 40.0%
CGAAGAAAGCTTGTCTAAGT+GGG + chr7_4:58054881-58054900 None:intergenic 40.0%
ACGAAGAAAGCTTGTCTAAG+TGG + chr7_4:58054882-58054901 None:intergenic 40.0%
GCTTAGTGGAGTGGAAAATA+AGG + chr7_4:58054906-58054925 None:intergenic 40.0%
GAGCAAAGAATAGATTCAGG+AGG + chr7_4:58055082-58055101 None:intergenic 40.0%
ATGTGATCATTCTACAGACG+TGG - chr7_4:58055135-58055154 Msa1166040:three_prime_UTR 40.0%
TGTGATCATTCTACAGACGT+GGG - chr7_4:58055136-58055155 Msa1166040:three_prime_UTR 40.0%
TCAAGTTTGCAACATAACCG+CGG + chr7_4:58055277-58055296 None:intergenic 40.0%
GAAGACAAATACAACTGAGG+CGG - chr7_4:58055311-58055330 Msa1166040:CDS 40.0%
GTTAAAGAGTAGTGTCTTGG+CGG - chr7_4:58055527-58055546 Msa1166040:CDS 40.0%
TTAAAGAGTAGTGTCTTGGC+GGG - chr7_4:58055528-58055547 Msa1166040:CDS 40.0%
TCCAACCGTTATGATTGTCA+CGG - chr7_4:58055861-58055880 Msa1166040:CDS 40.0%
! AATTGATTCTTGGCAGCACA+AGG - chr7_4:58055908-58055927 Msa1166040:CDS 40.0%
CAGGGAAATCAGTAGAACTT+TGG - chr7_4:58055985-58056004 Msa1166040:intron 40.0%
! GTTGGTGAAAGTAGTCATGA+AGG - chr7_4:58056116-58056135 Msa1166040:intron 40.0%
TGTGGATAGTACATATGTGG+TGG + chr7_4:58056434-58056453 None:intergenic 40.0%
TTATAGCTTAACCCATAGGG+TGG + chr7_4:58056476-58056495 None:intergenic 40.0%
! TAGCTGTTGATTGGATGAGA+GGG + chr7_4:58056596-58056615 None:intergenic 40.0%
! ATAGCTGTTGATTGGATGAG+AGG + chr7_4:58056597-58056616 None:intergenic 40.0%
TCTCATCCAATCAACAGCTA+TGG - chr7_4:58056596-58056615 Msa1166040:intron 40.0%
TGATGACCATAGCTGTTGAT+TGG + chr7_4:58056605-58056624 None:intergenic 40.0%
GACAAAGCTATACAATGTGG+AGG - chr7_4:58056669-58056688 Msa1166040:CDS 40.0%
GTGCTTAATGTAAGGGAAAG+TGG - chr7_4:58056730-58056749 Msa1166040:intron 40.0%
GCTTAATGTAAGGGAAAGTG+GGG - chr7_4:58056732-58056751 Msa1166040:intron 40.0%
CTTAATGTAAGGGAAAGTGG+GGG - chr7_4:58056733-58056752 Msa1166040:intron 40.0%
!! ATATGGTGTAGTTTGCTTGC+TGG - chr7_4:58056978-58056997 Msa1166040:five_prime_UTR 40.0%
! GCAGAGCATTACAATGCTAT+TGG + chr7_4:58057105-58057124 None:intergenic 40.0%
TCTAAGTGGGGTGTGTTTGT+TGG + chr7_4:58054868-58054887 None:intergenic 45.0%
! TTGGTACGTAGCTAGCTTAG+TGG + chr7_4:58054920-58054939 None:intergenic 45.0%
! TGTTGATCAGCAGCTGGTTT+GGG + chr7_4:58054968-58054987 None:intergenic 45.0%
TTGTTGATCAGCAGCTGGTT+TGG + chr7_4:58054969-58054988 None:intergenic 45.0%
! TTGAGTGTTTCTAATCCGGG+AGG + chr7_4:58054995-58055014 None:intergenic 45.0%
AAAGTCTTGATCCACTGCGA+CGG - chr7_4:58055164-58055183 Msa1166040:three_prime_UTR 45.0%
ATAGACACACACACACACCA+TGG - chr7_4:58055238-58055257 Msa1166040:CDS 45.0%
TCCGTGACAATCATAACGGT+TGG + chr7_4:58055865-58055884 None:intergenic 45.0%
CAGCACAAGGTTACAGTTAC+TGG - chr7_4:58055921-58055940 Msa1166040:CDS 45.0%
! TTCTTCTCAGGAGGCTTTTC+TGG + chr7_4:58056011-58056030 None:intergenic 45.0%
GATGCAATTGCAGGTGATGA+TGG - chr7_4:58056149-58056168 Msa1166040:intron 45.0%
GGGTGTGGATAGTACATATG+TGG + chr7_4:58056437-58056456 None:intergenic 45.0%
!! GTGTAGTTTGCTTGCTGGTA+TGG - chr7_4:58056983-58057002 Msa1166040:five_prime_UTR 45.0%
!! TAGTTTGCTTGCTGGTATGG+TGG - chr7_4:58056986-58057005 Msa1166040:five_prime_UTR 45.0%
! AGTTTGCTTGCTGGTATGGT+GGG - chr7_4:58056987-58057006 Msa1166040:five_prime_UTR 45.0%
! ACGTAGCTAGCTTAGTGGAG+TGG + chr7_4:58054915-58054934 None:intergenic 50.0%
!! GGAACTTGTTGATCAGCAGC+TGG + chr7_4:58054974-58054993 None:intergenic 50.0%
GCTGATCAACAAGTTCCTCC+CGG - chr7_4:58054977-58054996 Msa1166040:three_prime_UTR 50.0%
!! ATGGTTCTGAATTGCGACCG+CGG - chr7_4:58055257-58055276 Msa1166040:CDS 50.0%
! GCGGACTACAACCGCAATTA+TGG + chr7_4:58055375-58055394 None:intergenic 50.0%
GGTTACAGTTACTGGCAACG+TGG - chr7_4:58055929-58055948 Msa1166040:CDS 50.0%
GGGTTATAAGCATGCTGCTG+AGG + chr7_4:58056407-58056426 None:intergenic 50.0%
TAGCATGCATGGGCATGACA+GGG + chr7_4:58056544-58056563 None:intergenic 50.0%
TTAGCATGCATGGGCATGAC+AGG + chr7_4:58056545-58056564 None:intergenic 50.0%
TTGATTGGATGAGAGGGTGG+AGG + chr7_4:58056590-58056609 None:intergenic 50.0%
! CTGTTGATTGGATGAGAGGG+TGG + chr7_4:58056593-58056612 None:intergenic 50.0%
! TTTTTGTGCAGCCGTCGCAG+TGG + chr7_4:58055178-58055197 None:intergenic 55.0%
GCGGTCGCAATTCAGAACCA+TGG + chr7_4:58055258-58055277 None:intergenic 55.0%
! AGTAGTGTCTTGGCGGGTCT+TGG - chr7_4:58055534-58055553 Msa1166040:CDS 55.0%
!! TGCTGCTGAGGAAGTACTGG+AGG + chr7_4:58056395-58056414 None:intergenic 55.0%
! GCATGCTGCTGAGGAAGTAC+TGG + chr7_4:58056398-58056417 None:intergenic 55.0%
CAGCAGTAGCACCACCCTAT+GGG - chr7_4:58056462-58056481 Msa1166040:intron 55.0%
AGCATGCATGGGCATGACAG+GGG + chr7_4:58056543-58056562 None:intergenic 55.0%
ATTGGATGAGAGGGTGGAGG+AGG + chr7_4:58056587-58056606 None:intergenic 55.0%
!! GTGGTGCTACTGCTGGAGGA+GGG + chr7_4:58056457-58056476 None:intergenic 60.0%
!! TAGGGTGGTGCTACTGCTGG+AGG + chr7_4:58056461-58056480 None:intergenic 60.0%
!! CCATAGGGTGGTGCTACTGC+TGG + chr7_4:58056464-58056483 None:intergenic 60.0%
CCAGCAGTAGCACCACCCTA+TGG - chr7_4:58056461-58056480 Msa1166040:intron 60.0%
GCTACTGCTGGAGGAGGGTG+TGG + chr7_4:58056452-58056471 None:intergenic 65.0%
!! GGTGGTGCTACTGCTGGAGG+AGG + chr7_4:58056458-58056477 None:intergenic 65.0%
GAGAGGGTGGAGGAGGTGGA+GGG + chr7_4:58056580-58056599 None:intergenic 65.0%
TGAGAGGGTGGAGGAGGTGG+AGG + chr7_4:58056581-58056600 None:intergenic 65.0%
GGATGAGAGGGTGGAGGAGG+TGG + chr7_4:58056584-58056603 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 58054693 58057183 58054693 ID=Msa1166040;Name=Msa1166040
chr7_4 mRNA 58054693 58057183 58054693 ID=Msa1166040-mRNA-1;Parent=Msa1166040;Name=Msa1166040-mRNA-1;_AED=0.12;_eAED=0.12;_QI=263|1|1|1|1|1|3|513|306
chr7_4 exon 58054693 58055984 58054693 ID=Msa1166040-mRNA-1:exon:17715;Parent=Msa1166040-mRNA-1
chr7_4 exon 58056649 58056724 58056649 ID=Msa1166040-mRNA-1:exon:17714;Parent=Msa1166040-mRNA-1
chr7_4 exon 58056855 58057183 58056855 ID=Msa1166040-mRNA-1:exon:17713;Parent=Msa1166040-mRNA-1
chr7_4 five_prime_UTR 58056921 58057183 58056921 ID=Msa1166040-mRNA-1:five_prime_utr;Parent=Msa1166040-mRNA-1
chr7_4 CDS 58056855 58056920 58056855 ID=Msa1166040-mRNA-1:cds;Parent=Msa1166040-mRNA-1
chr7_4 CDS 58056649 58056724 58056649 ID=Msa1166040-mRNA-1:cds;Parent=Msa1166040-mRNA-1
chr7_4 CDS 58055206 58055984 58055206 ID=Msa1166040-mRNA-1:cds;Parent=Msa1166040-mRNA-1
chr7_4 three_prime_UTR 58054693 58055205 58054693 ID=Msa1166040-mRNA-1:three_prime_utr;Parent=Msa1166040-mRNA-1
Gene Sequence

>Msa1166040

ATGGCTGCACCCAAACCAGCTGCTGATCAACAAGTTCCTCCCGGATTAGAAACACTCAAGTATCAGACGTGGGTTTTAAAAGTCTTGATCCACTGCGACGGCTGCACAAAAAGAGTCAAGAAAATTCTTCAGGGAATTGAAGGGGTTTACAGAACAGAAATTGATTCTTGGCAGCACAAGGTTACAGTTACTGGCAACGTGGATGCTGAGACTCTCATCAAGAAGCTTTCAAGATCAGGGAAATCAGTAGAACTTTGGCCAGAAAAGCCTCCTGAGAAGAAAGATAAAAAATCTTCAAAATCAAAAGGTGAAACTGAAAATATTAATAAGGAGATCAAGGAAGATCACAAGAACAGTACTGAACAAGTTGGTGAAAGTAGTCATGAAGGTTGTATAGATGATGCAATTGCAGGTGATGATGGTGAAGAAGATTCAGATCACAAAGAAGGTCATAATGGTGATAATAACAATAAAAGTGAGGGTGGTGCTAAAAAGAAGAAGAAAAAGAAAAAGAAAAACAAAAGTGGTTCTGCTTCATTAACTCCAAATAATGGAGGAGGAAAAGAAATGATTAGTACAGAAACAATTGATTCACCACAAGTACTTGAAAAAGTGAATGCCATTCCTAAGGAAATTATTATGCCTCCAGTACTTCCTCAGCAGCATGCTTATAACCCATATCCACCACATATGTACTATCCACACCCTCCTCCAGCAGTAGCACCACCCTATGGGTTAAGCTATAACACATCATATCCTGTTTCTAGCACTTCTTCTTATTATGTTGGTGCCCCTGTCATGCCCATGCATGCTAATTACTCTAGATTCCCTCCACCTCCTCCACCCTCTCATCCAATCAACAGCTATGGTCATCATAATGATCATGAAGAATATGAAGGTGGATATTGCTCCATTATGTGA
Protein sequence

>Msa1166040

MAAPKPAADQQVPPGLETLKYQTWVLKVLIHCDGCTKRVKKILQGIEGVYRTEIDSWQHKVTVTGNVDAETLIKKLSRSGKSVELWPEKPPEKKDKKSSKSKGETENINKEIKEDHKNSTEQVGESSHEGCIDDAIAGDDGEEDSDHKEGHNGDNNNKSEGGAKKKKKKKKKNKSGSASLTPNNGGGKEMISTETIDSPQVLEKVNAIPKEIIMPPVLPQQHAYNPYPPHMYYPHPPPAVAPPYGLSYNTSYPVSSTSSYYVGAPVMPMHANYSRFPPPPPPSHPINSYGHHNDHEEYEGGYCSIM*