AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0684070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0684070 MtrunA17_Chr4g0031411 95.252 337 12 2 1 336 1 334 0.0 585
Msa0684070 MtrunA17_Chr7g0237921 44.144 333 129 10 1 312 1 297 1.64e-61 200
Msa0684070 MtrunA17_Chr2g0328021 38.889 360 124 15 1 336 1 288 4.93e-57 186
Msa0684070 MtrunA17_Chr4g0010151 38.857 350 154 12 1 317 1 323 9.92e-56 185
Msa0684070 MtrunA17_Chr7g0223311 59.028 144 52 4 1 139 1 142 1.44e-46 162
Msa0684070 MtrunA17_Chr5g0411951 87.500 56 7 0 70 125 38 93 9.88e-33 124
Msa0684070 MtrunA17_Chr3g0117601 54.867 113 44 3 48 160 37 142 1.42e-32 124
Msa0684070 MtrunA17_Chr4g0047001 66.667 81 23 1 76 156 56 132 4.76e-32 123
Msa0684070 MtrunA17_Chr2g0326001 80.328 61 12 0 69 129 34 94 1.47e-31 120
Msa0684070 MtrunA17_Chr8g0374251 60.674 89 26 1 75 163 68 147 1.60e-31 121
Msa0684070 MtrunA17_Chr4g0030051 80.645 62 11 1 67 127 32 93 3.66e-31 119
Msa0684070 MtrunA17_Chr8g0367161 69.620 79 20 2 74 152 31 105 8.72e-31 119
Msa0684070 MtrunA17_Chr8g0346551 57.732 97 39 1 62 158 11 105 9.51e-31 117
Msa0684070 MtrunA17_Chr8g0374111 54.717 106 32 3 54 159 17 106 6.77e-30 114
Msa0684070 MtrunA17_Chr3g0127201 66.216 74 21 1 56 129 12 81 7.96e-30 115
Msa0684070 MtrunA17_Chr1g0188851 60.000 80 30 1 52 129 18 97 2.80e-29 114
Msa0684070 MtrunA17_Chr3g0126201 63.514 74 27 0 56 129 32 105 7.50e-29 113
Msa0684070 MtrunA17_Chr8g0341671 58.750 80 30 1 59 135 1 80 1.17e-28 110
Msa0684070 MtrunA17_Chr2g0282441 67.606 71 22 1 57 126 6 76 1.80e-28 112
Msa0684070 MtrunA17_Chr4g0061761 82.692 52 9 0 74 125 39 90 2.75e-28 112
Msa0684070 MtrunA17_Chr2g0282511 88.000 50 6 0 74 123 44 93 4.41e-28 111
Msa0684070 MtrunA17_Chr1g0177251 63.636 77 27 1 77 153 14 89 4.88e-28 110
Msa0684070 MtrunA17_Chr2g0308011 84.000 50 8 0 74 123 36 85 6.27e-27 105
Msa0684070 MtrunA17_Chr4g0047761 71.186 59 15 1 75 133 72 128 1.20e-24 102
Msa0684070 MtrunA17_Chr2g0282051 66.129 62 16 1 74 135 45 101 1.63e-24 100
Msa0684070 MtrunA17_Chr8g0355261 66.102 59 20 0 71 129 114 172 2.23e-24 102
Msa0684070 MtrunA17_Chr2g0283811 63.380 71 25 1 72 142 44 113 2.69e-24 97.1
Msa0684070 MtrunA17_Chr6g0460721 63.333 60 22 0 68 127 82 141 5.88e-24 100
Msa0684070 MtrunA17_Chr6g0453321 66.667 60 20 0 68 127 129 188 8.52e-24 101
Msa0684070 MtrunA17_Chr4g0042481 58.571 70 26 1 58 127 108 174 1.44e-23 100
Msa0684070 MtrunA17_Chr7g0217581 49.412 85 43 0 68 152 225 309 1.55e-23 101
Msa0684070 MtrunA17_Chr7g0254161 60.000 70 25 2 60 129 88 154 1.94e-23 100
Msa0684070 MtrunA17_Chr3g0092111 62.687 67 24 1 68 134 129 194 2.46e-23 100
Msa0684070 MtrunA17_Chr6g0460731 61.667 60 23 0 68 127 89 148 3.82e-23 98.6
Msa0684070 MtrunA17_Chr5g0417571 43.820 89 50 0 39 127 102 190 1.11e-21 94.0
Msa0684070 MtrunA17_Chr5g0417601 69.091 55 16 1 77 131 142 195 1.18e-20 91.7
Msa0684070 MtrunA17_Chr5g0417551 48.780 82 41 1 59 140 111 191 4.79e-20 89.7
Msa0684070 MtrunA17_Chr5g0417561 50.725 69 34 0 59 127 103 171 1.02e-19 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0684070 AT3G55370.1 42.164 268 104 8 1 251 1 234 2.26e-51 173
Msa0684070 AT3G55370.2 42.164 268 104 8 1 251 1 234 2.41e-51 174
Msa0684070 AT3G55370.3 39.809 314 118 11 32 324 85 348 1.22e-48 167
Msa0684070 AT1G07640.1 42.308 286 145 10 57 335 3 275 1.77e-48 164
Msa0684070 AT1G07640.2 42.105 285 147 9 57 335 59 331 8.76e-48 164
Msa0684070 AT1G07640.3 42.105 285 147 9 57 335 67 339 9.60e-48 164
Msa0684070 AT2G37590.1 53.846 156 32 2 1 129 1 143 8.91e-47 161
Msa0684070 AT2G28810.1 36.685 368 160 15 1 332 1 331 1.35e-45 159
Msa0684070 AT5G02460.1 88.158 76 9 0 54 129 74 149 4.99e-44 156
Msa0684070 AT2G28810.2 38.776 294 125 11 57 332 58 314 1.68e-42 150
Msa0684070 AT5G60200.1 76.389 72 13 1 70 141 50 117 1.85e-34 127
Msa0684070 AT1G51700.1 58.889 90 29 1 70 159 28 109 1.29e-31 117
Msa0684070 AT5G60850.1 62.791 86 25 2 74 159 50 128 2.74e-31 120
Msa0684070 AT3G45610.1 53.409 88 39 1 70 155 35 122 3.09e-31 118
Msa0684070 AT4G38000.1 77.049 61 14 0 69 129 35 95 2.29e-30 116
Msa0684070 AT5G65590.1 81.034 58 11 0 75 132 41 98 4.58e-30 117
Msa0684070 AT3G21270.1 53.000 100 28 2 75 155 29 128 4.18e-29 111
Msa0684070 AT4G24060.1 50.000 112 56 0 69 180 47 158 1.92e-28 113
Msa0684070 AT1G28310.1 79.661 59 11 1 75 133 27 84 3.83e-28 111
Msa0684070 AT3G52440.1 70.588 68 16 1 77 144 27 90 4.24e-28 110
Msa0684070 AT3G52440.2 70.588 68 16 1 77 144 46 109 4.91e-28 110
Msa0684070 AT2G28510.1 73.846 65 13 1 74 138 47 107 5.54e-28 110
Msa0684070 AT1G28310.2 79.661 59 11 1 75 133 51 108 5.79e-28 111
Msa0684070 AT5G62940.1 77.193 57 13 0 69 125 67 123 7.85e-28 112
Msa0684070 AT3G61850.2 58.889 90 29 2 71 156 58 143 2.49e-27 108
Msa0684070 AT3G61850.3 58.889 90 29 2 71 156 58 143 2.49e-27 108
Msa0684070 AT5G66940.1 65.714 70 21 1 75 144 32 98 2.54e-27 107
Msa0684070 AT3G61850.1 58.889 90 29 2 71 156 70 155 3.13e-27 108
Msa0684070 AT3G61850.5 58.889 90 29 2 71 156 58 143 3.36e-27 109
Msa0684070 AT1G21340.1 71.875 64 12 1 74 137 37 94 3.53e-27 107
Msa0684070 AT3G61850.4 58.889 90 29 2 71 156 70 155 3.78e-27 109
Msa0684070 AT4G21050.1 47.581 124 50 3 68 190 17 126 4.47e-27 106
Msa0684070 AT3G50410.1 70.690 58 17 0 75 132 30 87 1.64e-26 105
Msa0684070 AT2G46590.1 56.250 80 35 0 43 122 36 115 2.86e-26 107
Msa0684070 AT2G46590.2 56.250 80 35 0 43 122 48 127 3.29e-26 107
Msa0684070 AT4G00940.1 63.514 74 23 2 59 129 50 122 1.30e-25 104
Msa0684070 AT4G00940.3 63.514 74 23 2 59 129 50 122 1.30e-25 104
Msa0684070 AT4G00940.2 63.514 74 23 2 59 129 50 122 1.30e-25 104
Msa0684070 AT1G64620.1 66.176 68 23 0 56 123 30 97 4.42e-25 104
Msa0684070 AT1G47655.1 70.000 60 16 1 66 123 18 77 2.02e-24 99.4
Msa0684070 AT2G34140.1 58.442 77 28 2 53 129 39 111 3.81e-24 97.4
Msa0684070 AT1G29160.1 69.492 59 17 1 71 129 58 115 1.22e-23 96.3
Msa0684070 AT4G21040.1 56.250 80 30 1 77 156 27 101 3.80e-23 96.3
Msa0684070 AT5G39660.3 66.667 63 20 1 67 129 130 191 3.80e-23 100
Msa0684070 AT5G39660.1 66.667 63 20 1 67 129 130 191 3.80e-23 100
Msa0684070 AT5G39660.2 66.667 63 20 1 67 129 130 191 3.80e-23 100
Msa0684070 AT1G26790.1 54.412 68 31 0 60 127 96 163 4.32e-23 99.0
Msa0684070 AT5G62430.1 56.000 75 31 2 58 131 36 109 4.51e-23 97.8
Msa0684070 AT3G47500.1 65.000 60 21 0 68 127 103 162 8.86e-23 99.0
Msa0684070 AT1G69570.1 61.667 60 23 0 68 127 125 184 2.18e-22 97.4
Msa0684070 AT4G21080.1 63.492 63 18 1 77 139 27 84 7.79e-22 93.2
Msa0684070 AT4G21030.1 54.098 61 25 1 68 128 17 74 2.03e-15 74.3

Find 112 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAATTTATGTACTATATA+TGG 0.188271 4_4:+47278435 Msa0684070:three_prime_UTR
GCAAGACATGTAGGCGTTAT+TGG 0.207030 4_4:+47277563 Msa0684070:CDS
GTTGTTGTTGATGTTGTTCT+TGG 0.217968 4_4:-47277855 None:intergenic
GATGAGAGAATGAGGGAATA+AGG 0.227115 4_4:+47276879 Msa0684070:five_prime_UTR
TCAAGAGCCACCGGTTAAAA+TGG 0.246428 4_4:+47278128 Msa0684070:CDS
TCTTGAGAGTTTGTTGAATT+AGG 0.247926 4_4:-47277673 None:intergenic
CCATTTGCTTGTTGATGATT+TGG 0.249048 4_4:-47277316 None:intergenic
ACCAACAACAATACTATTCT+TGG 0.250684 4_4:+47278208 Msa0684070:CDS
GTTTCTGCTTCTGCTGTTGC+TGG 0.257390 4_4:+47277940 Msa0684070:CDS
GATCATATGGGGTTTCAAAT+TGG 0.271163 4_4:+47277886 Msa0684070:CDS
TTTATAAAAGAAAACATAAA+AGG 0.276480 4_4:+47277043 Msa0684070:five_prime_UTR
TATTCTTGGAATGATTTATC+AGG 0.288988 4_4:+47278222 Msa0684070:CDS
TACTGGTGGCGGTGGTACTA+TGG 0.292815 4_4:+47277390 Msa0684070:CDS
TTTACCTGGTGGTGCCAATT+AGG 0.304320 4_4:-47277114 None:intergenic
CCTTCTCATCATGAACTAAT+TGG 0.327970 4_4:+47277724 Msa0684070:CDS
TTCATATCTTTGATCCATTC+CGG 0.333436 4_4:-47278276 None:intergenic
GTTATGTAGATCCTCCTAAT+TGG 0.350822 4_4:+47277100 Msa0684070:CDS
AACATTGAAGCTGCAGCATC+TGG 0.352153 4_4:+47278069 Msa0684070:CDS
TTCTCTTCTCTGTATTCCCT+TGG 0.355868 4_4:+47276982 Msa0684070:five_prime_UTR
AATTCCTAACCTACCCTTCA+TGG 0.365060 4_4:+47277765 Msa0684070:CDS
ACAACAACAACAAGATCATA+TGG 0.370266 4_4:+47277873 Msa0684070:CDS
ATGAGAGAATGAGGGAATAA+GGG 0.394979 4_4:+47276880 Msa0684070:five_prime_UTR
GTAATTTATGTACTATATAT+GGG 0.398137 4_4:+47278436 Msa0684070:three_prime_UTR
TCTGCTGTTGCTGGAGGTGT+TGG 0.398368 4_4:+47277949 Msa0684070:CDS
TTTCAAATTGGTATTGGTAA+TGG 0.399190 4_4:+47277898 Msa0684070:CDS
TCCAAGAATAGTATTGTTGT+TGG 0.406762 4_4:-47278209 None:intergenic
ATGGGGTTTCAAATTGGTAT+TGG 0.408013 4_4:+47277892 Msa0684070:CDS
AATACAGAGAAGAGAAAGAA+AGG 0.409902 4_4:-47276975 None:intergenic
CTACGACACCCTCCACCAAC+TGG 0.417773 4_4:-47277613 None:intergenic
CTACTCATCTCTTATAACTT+TGG 0.422294 4_4:+47278253 Msa0684070:exon
GGACACTTTAGCGCTGGTTC+TGG 0.424523 4_4:-47277469 None:intergenic
TGAGAAGGGATTGCACTTGA+TGG 0.431346 4_4:-47277709 None:intergenic
CTGACGTTGTAATATCAATT+CGG 0.439770 4_4:+47278528 Msa0684070:three_prime_UTR
GCTCGATCAGCCATGGAGCC+TGG 0.444337 4_4:-47277424 None:intergenic
CCTCAAGTGGGAGGTAGTAC+TGG 0.448394 4_4:+47277373 Msa0684070:CDS
CCAAATCATCAACAAGCAAA+TGG 0.450966 4_4:+47277316 Msa0684070:CDS
TTGATGGCTCTTTAGGGAGA+TGG 0.453212 4_4:-47277017 None:intergenic
GATATGAAGTTTCGAACGAT+TGG 0.454492 4_4:+47278291 Msa0684070:three_prime_UTR
AAACAAAATCAATTACTAGA+AGG 0.455741 4_4:-47278389 None:intergenic
TCTTATAACTTTGGCCGGAA+TGG 0.459551 4_4:+47278262 Msa0684070:exon
GATTCAAAAGTATTCAAGAA+AGG 0.460724 4_4:-47278003 None:intergenic
TTCTTGATAAATTTAAAGCT+CGG 0.472306 4_4:-47278158 None:intergenic
CTTAGAAATGTTCCAGTTGG+TGG 0.472325 4_4:+47277601 Msa0684070:CDS
CAACAACAACAAGATCATAT+GGG 0.473282 4_4:+47277874 Msa0684070:CDS
AATTTATCAAGAACATCTCT+TGG 0.474556 4_4:+47278168 Msa0684070:CDS
CTCGATCAGACCAGGCTCCA+TGG 0.475078 4_4:+47277414 Msa0684070:CDS
GAGATATAGCTTCAAACTCT+AGG 0.480948 4_4:+47278100 Msa0684070:CDS
CCTCCCACTTGAGGTGGTAA+AGG 0.483237 4_4:-47277364 None:intergenic
TTTAGCGCTGGTTCTGGTGG+TGG 0.483860 4_4:-47277463 None:intergenic
ATACAGAGAAGAGAAAGAAA+GGG 0.484195 4_4:-47276974 None:intergenic
GGTAGGTTAGGAATTGTTTG+TGG 0.484498 4_4:-47277757 None:intergenic
AGCATTAAAGGAAGAGAGGA+GGG 0.487565 4_4:-47276945 None:intergenic
GAGGCCATGAAGGGTAGGTT+AGG 0.490552 4_4:-47277769 None:intergenic
AGAAATGTTCCAGTTGGTGG+AGG 0.497986 4_4:+47277604 Msa0684070:CDS
AAAGGAAGAGAGGAGGGAGG+AGG 0.506532 4_4:-47276939 None:intergenic
TCATCTCTTATAACTTTGGC+CGG 0.512022 4_4:+47278257 Msa0684070:exon
CCAATTAGTTCATGATGAGA+AGG 0.516486 4_4:-47277724 None:intergenic
GCTGCAGCATCTGGATTCGT+TGG 0.518066 4_4:+47278078 Msa0684070:CDS
CACTTTAGCGCTGGTTCTGG+TGG 0.522746 4_4:-47277466 None:intergenic
GAAGTAGCAAAATTTAGTGT+TGG 0.523027 4_4:-47277506 None:intergenic
AGAAGTGACGAGGTTGAGTA+AGG 0.524057 4_4:-47277540 None:intergenic
TCCTCCTAATTGGCACCACC+AGG 0.528426 4_4:+47277110 Msa0684070:CDS
AAAGAGCATTAAAGGAAGAG+AGG 0.530419 4_4:-47276949 None:intergenic
ACTGGTGGCGGTGGTACTAT+GGG 0.530847 4_4:+47277391 Msa0684070:CDS
GGAAACGTTAACATGGGACT+TGG 0.531022 4_4:+47277820 Msa0684070:CDS
GAGAAGGGATTGCACTTGAT+GGG 0.532889 4_4:-47277708 None:intergenic
AGGAGGAAAGCAGCAATAAG+AGG 0.536872 4_4:-47276922 None:intergenic
CAACGAGGACACTTTAGCGC+TGG 0.540252 4_4:-47277475 None:intergenic
ACCTGGTGGTGCCAATTAGG+AGG 0.541280 4_4:-47277111 None:intergenic
TCTGCTTCTGCTGTTGCTGG+AGG 0.548891 4_4:+47277943 Msa0684070:CDS
GAGCATTAAAGGAAGAGAGG+AGG 0.549927 4_4:-47276946 None:intergenic
ATGGCTCTTTAGGGAGATGG+TGG 0.554378 4_4:-47277014 None:intergenic
AGATATAGCTTCAAACTCTA+GGG 0.556521 4_4:+47278101 Msa0684070:CDS
GGAGGTAGTACTGGTGGCGG+TGG 0.560481 4_4:+47277382 Msa0684070:CDS
ATTCTGCATCGAGGCCATGA+AGG 0.561082 4_4:-47277779 None:intergenic
AATCTGAGTCGTTATGTCGT+TGG 0.570962 4_4:+47277799 Msa0684070:CDS
CAATTAGTTCATGATGAGAA+GGG 0.573984 4_4:-47277723 None:intergenic
TGTCGTTGGAAACGTTAACA+TGG 0.575317 4_4:+47277813 Msa0684070:CDS
TCCACCTTTACCACCTCAAG+TGG 0.577272 4_4:+47277360 Msa0684070:CDS
ACTATGGGCTCGATCAGACC+AGG 0.580839 4_4:+47277406 Msa0684070:CDS
TTCTGCATCGAGGCCATGAA+GGG 0.581643 4_4:-47277778 None:intergenic
CCCACTTGAGGTGGTAAAGG+TGG 0.581750 4_4:-47277361 None:intergenic
ATTAAAGGAAGAGAGGAGGG+AGG 0.592350 4_4:-47276942 None:intergenic
GAAATGTTCCAGTTGGTGGA+GGG 0.594932 4_4:+47277605 Msa0684070:CDS
GCACTTAGAAATGTTCCAGT+TGG 0.600640 4_4:+47277598 Msa0684070:CDS
TATATTATTCAACTCAGTCA+TGG 0.602165 4_4:+47278339 Msa0684070:three_prime_UTR
AGAAGGGATTGCACTTGATG+GGG 0.604506 4_4:-47277707 None:intergenic
GTAGGCGTTATTGGACAAGA+GGG 0.605161 4_4:+47277572 Msa0684070:CDS
TAGGGCTAATCAAGAGCCAC+CGG 0.605644 4_4:+47278119 Msa0684070:CDS
GTCGTTGGAAACGTTAACAT+GGG 0.609381 4_4:+47277814 Msa0684070:CDS
GTCACTTCTGCAAGACATGT+AGG 0.611130 4_4:+47277554 Msa0684070:CDS
TGTAGGCGTTATTGGACAAG+AGG 0.616737 4_4:+47277571 Msa0684070:CDS
GCATCGAGGCCATGAAGGGT+AGG 0.623817 4_4:-47277774 None:intergenic
AATTGCTGCAAATTACGCCA+TGG 0.629104 4_4:-47277979 None:intergenic
TAAACGAGCTCGATCAGCCA+TGG 0.633447 4_4:-47277431 None:intergenic
CATGTCTTGCAGAAGTGACG+AGG 0.635723 4_4:-47277550 None:intergenic
GTGGGAGGTAGTACTGGTGG+CGG 0.638124 4_4:+47277379 Msa0684070:CDS
AACAACAACAAGATCATATG+GGG 0.646211 4_4:+47277875 Msa0684070:CDS
CCACCTTTACCACCTCAAGT+GGG 0.662217 4_4:+47277361 Msa0684070:CDS
CAAGTGGGAGGTAGTACTGG+TGG 0.664836 4_4:+47277376 Msa0684070:CDS
GTACTACCTCCCACTTGAGG+TGG 0.666584 4_4:-47277370 None:intergenic
TAGGCGTTATTGGACAAGAG+GGG 0.675252 4_4:+47277573 Msa0684070:CDS
AGGCGTTATTGGACAAGAGG+GGG 0.675862 4_4:+47277574 Msa0684070:CDS
ATGGTGGAGAATCAAACCAA+GGG 0.681127 4_4:-47276998 None:intergenic
CCAGTACTACCTCCCACTTG+AGG 0.681370 4_4:-47277373 None:intergenic
GATGGTGGAGAATCAAACCA+AGG 0.687483 4_4:-47276999 None:intergenic
CCTTTACCACCTCAAGTGGG+AGG 0.700568 4_4:+47277364 Msa0684070:CDS
GAGGTGTTGGAGTTGAACCA+TGG 0.701694 4_4:+47277962 Msa0684070:CDS
TGAGTTGAATAATATATGAG+TGG 0.703462 4_4:-47278332 None:intergenic
TTGAATAGAAGAGAAAACCA+TGG 0.707467 4_4:-47277076 None:intergenic
GTGTTGGTTGAATCGCAACG+AGG 0.733913 4_4:-47277490 None:intergenic
ACGACTCAGATTCTGCATCG+AGG 0.741695 4_4:-47277788 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATAAAAGAAAACATAAA+AGG + chr4_4:47277043-47277062 Msa0684070:five_prime_UTR 10.0%
!!! ATTTTAAAAAATTATTGTGA+TGG - chr4_4:47277253-47277272 None:intergenic 10.0%
!!! ATAAGCTTCTTTTTTTTAAT+GGG - chr4_4:47276804-47276823 None:intergenic 15.0%
!!! TATAAGCTTCTTTTTTTTAA+TGG - chr4_4:47276805-47276824 None:intergenic 15.0%
!! ATTAAAAAAAAGAAGCTTAT+AGG + chr4_4:47276804-47276823 Msa0684070:five_prime_UTR 15.0%
!!! TTTCTTTTATAAAGTTTTGA+TGG - chr4_4:47277036-47277055 None:intergenic 15.0%
!! ATGGAAAGAAATTAAAAAAA+GGG - chr4_4:47277277-47277296 None:intergenic 15.0%
!!! GTTTATGTTTTTTTATTTCA+TGG + chr4_4:47278408-47278427 Msa0684070:three_prime_UTR 15.0%
!! TGTAATTTATGTACTATATA+TGG + chr4_4:47278435-47278454 Msa0684070:three_prime_UTR 15.0%
!! GTAATTTATGTACTATATAT+GGG + chr4_4:47278436-47278455 Msa0684070:three_prime_UTR 15.0%
!!! ATATTTTTTAGGGTTTTTAG+TGG - chr4_4:47276844-47276863 None:intergenic 20.0%
!! ATGAAAATTAAAACTTTACC+TGG - chr4_4:47277131-47277150 None:intergenic 20.0%
!! AATTCCAAATTCAAAAAAGA+AGG + chr4_4:47277153-47277172 Msa0684070:intron 20.0%
!! GATGGAAAGAAATTAAAAAA+AGG - chr4_4:47277278-47277297 None:intergenic 20.0%
!!! AATAATTCATACACTTTTCA+TGG + chr4_4:47278039-47278058 Msa0684070:CDS 20.0%
!!! TTCTTGATAAATTTAAAGCT+CGG - chr4_4:47278161-47278180 None:intergenic 20.0%
!! AAACAAAATCAATTACTAGA+AGG - chr4_4:47278392-47278411 None:intergenic 20.0%
!! TAAAAGGATTTTCAATACCA+TGG + chr4_4:47277059-47277078 Msa0684070:exon 25.0%
! AAAATTAAAACTTTACCTGG+TGG - chr4_4:47277128-47277147 None:intergenic 25.0%
!!! TTCACCTTCTTTTTTGAATT+TGG - chr4_4:47277160-47277179 None:intergenic 25.0%
! ATATTCAAGAAGATGAAGTA+AGG - chr4_4:47277217-47277236 None:intergenic 25.0%
!! TTGAATTAGGAGATTTTGAA+CGG - chr4_4:47277663-47277682 None:intergenic 25.0%
!!! TTTCAAATTGGTATTGGTAA+TGG + chr4_4:47277898-47277917 Msa0684070:CDS 25.0%
!!! GATTCAAAAGTATTCAAGAA+AGG - chr4_4:47278006-47278025 None:intergenic 25.0%
! AATTTATCAAGAACATCTCT+TGG + chr4_4:47278168-47278187 Msa0684070:CDS 25.0%
! TATTCTTGGAATGATTTATC+AGG + chr4_4:47278222-47278241 Msa0684070:CDS 25.0%
!! TGAGTTGAATAATATATGAG+TGG - chr4_4:47278335-47278354 None:intergenic 25.0%
! TATATTATTCAACTCAGTCA+TGG + chr4_4:47278339-47278358 Msa0684070:three_prime_UTR 25.0%
!!! GCTTCTTTTTTTTAATGGGT+TGG - chr4_4:47276800-47276819 None:intergenic 30.0%
!!! TTTTTTAGGGTTTTTAGTGG+AGG - chr4_4:47276841-47276860 None:intergenic 30.0%
AAAAAGAGATGAGAGAATGA+GGG + chr4_4:47276872-47276891 Msa0684070:five_prime_UTR 30.0%
!! AAAGGGAAAAAGAGCATTAA+AGG - chr4_4:47276960-47276979 None:intergenic 30.0%
ATACAGAGAAGAGAAAGAAA+GGG - chr4_4:47276977-47276996 None:intergenic 30.0%
AATACAGAGAAGAGAAAGAA+AGG - chr4_4:47276978-47276997 None:intergenic 30.0%
!! AAAGTTTTGATGGCTCTTTA+GGG - chr4_4:47277026-47277045 None:intergenic 30.0%
!! TAAAGTTTTGATGGCTCTTT+AGG - chr4_4:47277027-47277046 None:intergenic 30.0%
TTGAATAGAAGAGAAAACCA+TGG - chr4_4:47277079-47277098 None:intergenic 30.0%
! ATGGTTTTCTCTTCTATTCA+AGG + chr4_4:47277078-47277097 Msa0684070:CDS 30.0%
GAAGTAGCAAAATTTAGTGT+TGG - chr4_4:47277509-47277528 None:intergenic 30.0%
!! TCTTGAGAGTTTGTTGAATT+AGG - chr4_4:47277676-47277695 None:intergenic 30.0%
CAATTAGTTCATGATGAGAA+GGG - chr4_4:47277726-47277745 None:intergenic 30.0%
ACAACAACAACAAGATCATA+TGG + chr4_4:47277873-47277892 Msa0684070:CDS 30.0%
CAACAACAACAAGATCATAT+GGG + chr4_4:47277874-47277893 Msa0684070:CDS 30.0%
AACAACAACAAGATCATATG+GGG + chr4_4:47277875-47277894 Msa0684070:CDS 30.0%
! ACTTTTGAATCAAACTCTGA+TGG + chr4_4:47278015-47278034 Msa0684070:CDS 30.0%
AGATATAGCTTCAAACTCTA+GGG + chr4_4:47278101-47278120 Msa0684070:CDS 30.0%
ACCAACAACAATACTATTCT+TGG + chr4_4:47278208-47278227 Msa0684070:CDS 30.0%
TCCAAGAATAGTATTGTTGT+TGG - chr4_4:47278212-47278231 None:intergenic 30.0%
CTACTCATCTCTTATAACTT+TGG + chr4_4:47278253-47278272 Msa0684070:exon 30.0%
TTCATATCTTTGATCCATTC+CGG - chr4_4:47278279-47278298 None:intergenic 30.0%
CTGACGTTGTAATATCAATT+CGG + chr4_4:47278528-47278547 Msa0684070:three_prime_UTR 30.0%
CAAAAAGAGATGAGAGAATG+AGG + chr4_4:47276871-47276890 Msa0684070:five_prime_UTR 35.0%
ATGAGAGAATGAGGGAATAA+GGG + chr4_4:47276880-47276899 Msa0684070:five_prime_UTR 35.0%
!! AAAGAGCATTAAAGGAAGAG+AGG - chr4_4:47276952-47276971 None:intergenic 35.0%
GTTATGTAGATCCTCCTAAT+TGG + chr4_4:47277100-47277119 Msa0684070:CDS 35.0%
TTGCTGCAGGTTAAAATAGA+TGG - chr4_4:47277296-47277315 None:intergenic 35.0%
!! CCATTTGCTTGTTGATGATT+TGG - chr4_4:47277319-47277338 None:intergenic 35.0%
CCAAATCATCAACAAGCAAA+TGG + chr4_4:47277316-47277335 Msa0684070:CDS 35.0%
CCAATTAGTTCATGATGAGA+AGG - chr4_4:47277727-47277746 None:intergenic 35.0%
CCTTCTCATCATGAACTAAT+TGG + chr4_4:47277724-47277743 Msa0684070:CDS 35.0%
! GTTGTTGTTGATGTTGTTCT+TGG - chr4_4:47277858-47277877 None:intergenic 35.0%
GATCATATGGGGTTTCAAAT+TGG + chr4_4:47277886-47277905 Msa0684070:CDS 35.0%
ATGGGGTTTCAAATTGGTAT+TGG + chr4_4:47277892-47277911 Msa0684070:CDS 35.0%
GAGATATAGCTTCAAACTCT+AGG + chr4_4:47278100-47278119 Msa0684070:CDS 35.0%
!! TTTTCTTCCATTTTAACCGG+TGG - chr4_4:47278138-47278157 None:intergenic 35.0%
!! CGGTTTTCTTCCATTTTAAC+CGG - chr4_4:47278141-47278160 None:intergenic 35.0%
TCATCTCTTATAACTTTGGC+CGG + chr4_4:47278257-47278276 Msa0684070:exon 35.0%
GATATGAAGTTTCGAACGAT+TGG + chr4_4:47278291-47278310 Msa0684070:three_prime_UTR 35.0%
GATGAGAGAATGAGGGAATA+AGG + chr4_4:47276879-47276898 Msa0684070:five_prime_UTR 40.0%
AGCATTAAAGGAAGAGAGGA+GGG - chr4_4:47276948-47276967 None:intergenic 40.0%
TTCTCTTCTCTGTATTCCCT+TGG + chr4_4:47276982-47277001 Msa0684070:five_prime_UTR 40.0%
! ATGGTGGAGAATCAAACCAA+GGG - chr4_4:47277001-47277020 None:intergenic 40.0%
GCACTTAGAAATGTTCCAGT+TGG + chr4_4:47277598-47277617 Msa0684070:CDS 40.0%
CTTAGAAATGTTCCAGTTGG+TGG + chr4_4:47277601-47277620 Msa0684070:CDS 40.0%
GGTAGGTTAGGAATTGTTTG+TGG - chr4_4:47277760-47277779 None:intergenic 40.0%
AATTCCTAACCTACCCTTCA+TGG + chr4_4:47277765-47277784 Msa0684070:CDS 40.0%
AATCTGAGTCGTTATGTCGT+TGG + chr4_4:47277799-47277818 Msa0684070:CDS 40.0%
TGTCGTTGGAAACGTTAACA+TGG + chr4_4:47277813-47277832 Msa0684070:CDS 40.0%
GTCGTTGGAAACGTTAACAT+GGG + chr4_4:47277814-47277833 Msa0684070:CDS 40.0%
AATTGCTGCAAATTACGCCA+TGG - chr4_4:47277982-47278001 None:intergenic 40.0%
TCTTATAACTTTGGCCGGAA+TGG + chr4_4:47278262-47278281 Msa0684070:exon 40.0%
AGGAGGAAAGCAGCAATAAG+AGG - chr4_4:47276925-47276944 None:intergenic 45.0%
ATTAAAGGAAGAGAGGAGGG+AGG - chr4_4:47276945-47276964 None:intergenic 45.0%
GAGCATTAAAGGAAGAGAGG+AGG - chr4_4:47276949-47276968 None:intergenic 45.0%
!! GATGGTGGAGAATCAAACCA+AGG - chr4_4:47277002-47277021 None:intergenic 45.0%
! TTGATGGCTCTTTAGGGAGA+TGG - chr4_4:47277020-47277039 None:intergenic 45.0%
! TTTACCTGGTGGTGCCAATT+AGG - chr4_4:47277117-47277136 None:intergenic 45.0%
!! GTTGATGATTTGGTTGCTGC+AGG - chr4_4:47277309-47277328 None:intergenic 45.0%
AGAAGTGACGAGGTTGAGTA+AGG - chr4_4:47277543-47277562 None:intergenic 45.0%
GTCACTTCTGCAAGACATGT+AGG + chr4_4:47277554-47277573 Msa0684070:CDS 45.0%
GCAAGACATGTAGGCGTTAT+TGG + chr4_4:47277563-47277582 Msa0684070:CDS 45.0%
TGTAGGCGTTATTGGACAAG+AGG + chr4_4:47277571-47277590 Msa0684070:CDS 45.0%
GTAGGCGTTATTGGACAAGA+GGG + chr4_4:47277572-47277591 Msa0684070:CDS 45.0%
TAGGCGTTATTGGACAAGAG+GGG + chr4_4:47277573-47277592 Msa0684070:CDS 45.0%
!! AGAAATGTTCCAGTTGGTGG+AGG + chr4_4:47277604-47277623 Msa0684070:CDS 45.0%
!! GAAATGTTCCAGTTGGTGGA+GGG + chr4_4:47277605-47277624 Msa0684070:CDS 45.0%
AGAAGGGATTGCACTTGATG+GGG - chr4_4:47277710-47277729 None:intergenic 45.0%
GAGAAGGGATTGCACTTGAT+GGG - chr4_4:47277711-47277730 None:intergenic 45.0%
TGAGAAGGGATTGCACTTGA+TGG - chr4_4:47277712-47277731 None:intergenic 45.0%
GGAAACGTTAACATGGGACT+TGG + chr4_4:47277820-47277839 Msa0684070:CDS 45.0%
AACATTGAAGCTGCAGCATC+TGG + chr4_4:47278069-47278088 Msa0684070:CDS 45.0%
! TCAAGAGCCACCGGTTAAAA+TGG + chr4_4:47278128-47278147 Msa0684070:CDS 45.0%
!!! TTGTTTTATTATATTTTTTA+GGG - chr4_4:47276854-47276873 None:intergenic 5.0%
!!! TTTGTTTTATTATATTTTTT+AGG - chr4_4:47276855-47276874 None:intergenic 5.0%
! ATGGCTCTTTAGGGAGATGG+TGG - chr4_4:47277017-47277036 None:intergenic 50.0%
TCCACCTTTACCACCTCAAG+TGG + chr4_4:47277360-47277379 Msa0684070:CDS 50.0%
CCACCTTTACCACCTCAAGT+GGG + chr4_4:47277361-47277380 Msa0684070:CDS 50.0%
TAAACGAGCTCGATCAGCCA+TGG - chr4_4:47277434-47277453 None:intergenic 50.0%
!! GTGTTGGTTGAATCGCAACG+AGG - chr4_4:47277493-47277512 None:intergenic 50.0%
CATGTCTTGCAGAAGTGACG+AGG - chr4_4:47277553-47277572 None:intergenic 50.0%
AGGCGTTATTGGACAAGAGG+GGG + chr4_4:47277574-47277593 Msa0684070:CDS 50.0%
TTCTGCATCGAGGCCATGAA+GGG - chr4_4:47277781-47277800 None:intergenic 50.0%
ATTCTGCATCGAGGCCATGA+AGG - chr4_4:47277782-47277801 None:intergenic 50.0%
ACGACTCAGATTCTGCATCG+AGG - chr4_4:47277791-47277810 None:intergenic 50.0%
GTTTCTGCTTCTGCTGTTGC+TGG + chr4_4:47277940-47277959 Msa0684070:CDS 50.0%
!! GAGGTGTTGGAGTTGAACCA+TGG + chr4_4:47277962-47277981 Msa0684070:CDS 50.0%
TAGGGCTAATCAAGAGCCAC+CGG + chr4_4:47278119-47278138 Msa0684070:CDS 50.0%
AAAGGAAGAGAGGAGGGAGG+AGG - chr4_4:47276942-47276961 None:intergenic 55.0%
TCCTCCTAATTGGCACCACC+AGG + chr4_4:47277110-47277129 Msa0684070:CDS 55.0%
! ACCTGGTGGTGCCAATTAGG+AGG - chr4_4:47277114-47277133 None:intergenic 55.0%
CCCACTTGAGGTGGTAAAGG+TGG - chr4_4:47277364-47277383 None:intergenic 55.0%
CCTCCCACTTGAGGTGGTAA+AGG - chr4_4:47277367-47277386 None:intergenic 55.0%
CCTTTACCACCTCAAGTGGG+AGG + chr4_4:47277364-47277383 Msa0684070:CDS 55.0%
GTACTACCTCCCACTTGAGG+TGG - chr4_4:47277373-47277392 None:intergenic 55.0%
CCAGTACTACCTCCCACTTG+AGG - chr4_4:47277376-47277395 None:intergenic 55.0%
CCTCAAGTGGGAGGTAGTAC+TGG + chr4_4:47277373-47277392 Msa0684070:CDS 55.0%
! CAAGTGGGAGGTAGTACTGG+TGG + chr4_4:47277376-47277395 Msa0684070:CDS 55.0%
!! TACTGGTGGCGGTGGTACTA+TGG + chr4_4:47277390-47277409 Msa0684070:CDS 55.0%
!! ACTGGTGGCGGTGGTACTAT+GGG + chr4_4:47277391-47277410 Msa0684070:CDS 55.0%
ACTATGGGCTCGATCAGACC+AGG + chr4_4:47277406-47277425 Msa0684070:CDS 55.0%
!! TTTAGCGCTGGTTCTGGTGG+TGG - chr4_4:47277466-47277485 None:intergenic 55.0%
!! CACTTTAGCGCTGGTTCTGG+TGG - chr4_4:47277469-47277488 None:intergenic 55.0%
!! GGACACTTTAGCGCTGGTTC+TGG - chr4_4:47277472-47277491 None:intergenic 55.0%
CAACGAGGACACTTTAGCGC+TGG - chr4_4:47277478-47277497 None:intergenic 55.0%
GAGGCCATGAAGGGTAGGTT+AGG - chr4_4:47277772-47277791 None:intergenic 55.0%
TCTGCTTCTGCTGTTGCTGG+AGG + chr4_4:47277943-47277962 Msa0684070:CDS 55.0%
! TCTGCTGTTGCTGGAGGTGT+TGG + chr4_4:47277949-47277968 Msa0684070:CDS 55.0%
GCTGCAGCATCTGGATTCGT+TGG + chr4_4:47278078-47278097 Msa0684070:CDS 55.0%
!! GTGGGAGGTAGTACTGGTGG+CGG + chr4_4:47277379-47277398 Msa0684070:CDS 60.0%
CTCGATCAGACCAGGCTCCA+TGG + chr4_4:47277414-47277433 Msa0684070:CDS 60.0%
CTACGACACCCTCCACCAAC+TGG - chr4_4:47277616-47277635 None:intergenic 60.0%
GCATCGAGGCCATGAAGGGT+AGG - chr4_4:47277777-47277796 None:intergenic 60.0%
!! GGAGGTAGTACTGGTGGCGG+TGG + chr4_4:47277382-47277401 Msa0684070:CDS 65.0%
GCTCGATCAGCCATGGAGCC+TGG - chr4_4:47277427-47277446 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 47276786 47278563 47276786 ID=Msa0684070;Name=Msa0684070
chr4_4 mRNA 47276786 47278563 47276786 ID=Msa0684070-mRNA-1;Parent=Msa0684070;Name=Msa0684070-mRNA-1;_AED=0.01;_eAED=0.01;_QI=292|1|1|1|1|1|2|294|336
chr4_4 exon 47276786 47277131 47276786 ID=Msa0684070-mRNA-1:exon:11638;Parent=Msa0684070-mRNA-1
chr4_4 exon 47277313 47278563 47277313 ID=Msa0684070-mRNA-1:exon:11639;Parent=Msa0684070-mRNA-1
chr4_4 five_prime_UTR 47276786 47277077 47276786 ID=Msa0684070-mRNA-1:five_prime_utr;Parent=Msa0684070-mRNA-1
chr4_4 CDS 47277078 47277131 47277078 ID=Msa0684070-mRNA-1:cds;Parent=Msa0684070-mRNA-1
chr4_4 CDS 47277313 47278269 47277313 ID=Msa0684070-mRNA-1:cds;Parent=Msa0684070-mRNA-1
chr4_4 three_prime_UTR 47278270 47278563 47278270 ID=Msa0684070-mRNA-1:three_prime_utr;Parent=Msa0684070-mRNA-1
Gene Sequence

>Msa0684070

ATGGTTTTCTCTTCTATTCAAGGTTATGTAGATCCTCCTAATTGGCACCACCAGCAACCAAATCATCAACAAGCAAATGGAAGCAATAACACTCAACTTCTTCCACCTTTACCACCTCAAGTGGGAGGTAGTACTGGTGGCGGTGGTACTATGGGCTCGATCAGACCAGGCTCCATGGCTGATCGAGCTCGTTTAGCAAAACTACCACCACCAGAACCAGCGCTAAAGTGTCCTCGTTGCGATTCAACCAACACTAAATTTTGCTACTTCAACAACTACAGCCTTACTCAACCTCGTCACTTCTGCAAGACATGTAGGCGTTATTGGACAAGAGGGGGTGCACTTAGAAATGTTCCAGTTGGTGGAGGGTGTCGTAGAAACAACAAGAAAAACAAAAGAAGCCGTTCAAAATCTCCTAATTCAACAAACTCTCAAGAAAAACAAACTTCCCCATCAAGTGCAATCCCTTCTCATCATGAACTAATTGGTCATCAGTTACCACAAACAATTCCTAACCTACCCTTCATGGCCTCGATGCAGAATCTGAGTCGTTATGTCGTTGGAAACGTTAACATGGGACTTGGTTTGCGTGAGATCCAAGAACAACATCAACAACAACAACAACAAGATCATATGGGGTTTCAAATTGGTATTGGTAATGGTAACTCAACTTCTGTTTCTGTTTCTGCTTCTGCTGTTGCTGGAGGTGTTGGAGTTGAACCATGGCGTAATTTGCAGCAATTTCCTTTCTTGAATACTTTTGAATCAAACTCTGATGGAAATAATTCATACACTTTTCATGGAGAAAGTAACATTGAAGCTGCAGCATCTGGATTCGTTGGAGATATAGCTTCAAACTCTAGGGCTAATCAAGAGCCACCGGTTAAAATGGAAGAAAACCGAGCTTTAAATTTATCAAGAACATCTCTTGGTGTCTCTGAACATAATAACCAACAACAATACTATTCTTGGAATGATTTATCAGGTTCTTCAACTACTCATCTCTTATAA
Protein sequence

>Msa0684070

MVFSSIQGYVDPPNWHHQQPNHQQANGSNNTQLLPPLPPQVGGSTGGGGTMGSIRPGSMADRARLAKLPPPEPALKCPRCDSTNTKFCYFNNYSLTQPRHFCKTCRRYWTRGGALRNVPVGGGCRRNNKKNKRSRSKSPNSTNSQEKQTSPSSAIPSHHELIGHQLPQTIPNLPFMASMQNLSRYVVGNVNMGLGLREIQEQHQQQQQQDHMGFQIGIGNGNSTSVSVSASAVAGGVGVEPWRNLQQFPFLNTFESNSDGNNSYTFHGESNIEAAASGFVGDIASNSRANQEPPVKMEENRALNLSRTSLGVSEHNNQQQYYSWNDLSGSSTTHLL*