AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0585600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585600 MtrunA17_Chr4g0001551 96.429 56 2 0 60 115 1 56 1.44e-35 116
Msa0585600 MtrunA17_Chr2g0333951 60.345 58 21 1 60 115 1 58 8.17e-17 68.9
Msa0585600 MtrunA17_Chr5g0399131 47.368 57 26 1 63 115 5 61 9.52e-12 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585600 AT4G25225.1 56.364 55 24 0 60 114 1 55 1.13e-16 68.6
Msa0585600 AT4G25225.2 52.381 63 30 0 52 114 27 89 2.14e-16 68.9
Msa0585600 AT2G35736.2 56.364 55 23 1 60 114 1 54 2.44e-14 62.8
Msa0585600 AT2G35736.1 56.364 55 23 1 60 114 1 54 2.44e-14 62.8

Find 36 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACATTTGCCTTGAATAA+TGG 0.223367 4_2:+6614052 None:intergenic
TAATCATCATCTTTCTTGTT+AGG 0.262346 4_2:+6614246 None:intergenic
CATCTTTCTTGTTAGGTTTG+TGG 0.329339 4_2:+6614253 None:intergenic
TCGGCTTCCTGCTTGATGTT+TGG 0.333657 4_2:+6614315 None:intergenic
CGATTTGTCCTCTATTATAT+AGG 0.338096 4_2:-6614156 Msa0585600:three_prime_UTR
AAGCCGACAATTTCTTAGAT+TGG 0.365488 4_2:-6614299 Msa0585600:CDS
CCTGCTTGATGTTTGGAACT+TGG 0.373685 4_2:+6614322 None:intergenic
CTTAGATTGGGCCAAGGAAA+TGG 0.379042 4_2:-6614286 Msa0585600:CDS
CTCCTGATTATACCCATTAC+AGG 0.380933 4_2:+6614399 None:intergenic
AGCCGACAATTTCTTAGATT+GGG 0.389747 4_2:-6614298 Msa0585600:CDS
GAGAGTGCGAAGTTGGTTTA+CGG 0.398722 4_2:-6615682 Msa0585600:CDS
TTGACACTACCAGTGGAATC+AGG 0.403559 4_2:-6614204 Msa0585600:three_prime_UTR
TAACTGACACGAATTAGTTA+AGG 0.430203 4_2:-6614118 Msa0585600:three_prime_UTR
TCGGAGATTATCACCGTTGC+CGG 0.433679 4_2:-6615738 Msa0585600:CDS
TTTATAGCAGGGAATGTGGT+TGG 0.437769 4_2:-6614366 Msa0585600:CDS
TTATAGCAGGGAATGTGGTT+GGG 0.445588 4_2:-6614365 Msa0585600:CDS
CGGCAGAGAGAGTGCGAAGT+TGG 0.449867 4_2:-6615689 Msa0585600:CDS
AAGGACTCAAGCACCTGTAA+TGG 0.454775 4_2:-6614412 Msa0585600:CDS
AGGACTCAAGCACCTGTAAT+GGG 0.461072 4_2:-6614411 Msa0585600:CDS
CACCTGTAATGGGTATAATC+AGG 0.464078 4_2:-6614401 Msa0585600:CDS
TCTCTCTCCGTCTCCGGCAA+CGG 0.468537 4_2:+6615725 None:intergenic
TTCTCAGTTACTGCAAATCA+AGG 0.497235 4_2:-6614431 Msa0585600:CDS
GGCCCAATCTAAGAAATTGT+CGG 0.500410 4_2:+6614296 None:intergenic
ATAAACATGTCCATTATTCA+AGG 0.510042 4_2:-6614062 None:intergenic
CCGTTGTCTCTCTCCGTCTC+CGG 0.534610 4_2:+6615719 None:intergenic
TACTTGATCCTATATAATAG+AGG 0.534716 4_2:+6614148 None:intergenic
AGAGAGACAACGGTGAGTTA+CGG 0.563877 4_2:-6615709 Msa0585600:CDS
CCAAGTTCCAAACATCAAGC+AGG 0.570183 4_2:-6614322 Msa0585600:CDS
ATTATCACCGTTGCCGGAGA+CGG 0.578694 4_2:-6615732 Msa0585600:CDS
TTGTTTGAGGAGGAATGTAT+CGG 0.582328 4_2:-6615757 None:intergenic
CAATTTCTTAGATTGGGCCA+AGG 0.608950 4_2:-6614292 Msa0585600:CDS
AAAACAAAACCTGATTCCAC+TGG 0.620858 4_2:+6614195 None:intergenic
AACTTCTTTGACACTACCAG+TGG 0.621466 4_2:-6614211 Msa0585600:three_prime_UTR
GAATGTGGTTGGGATCTATG+TGG 0.655810 4_2:-6614355 Msa0585600:CDS
CTGTAATGGGTATAATCAGG+AGG 0.664923 4_2:-6614398 Msa0585600:CDS
CCGGAGACGGAGAGAGACAA+CGG 0.683444 4_2:-6615719 Msa0585600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATAAATATGAAAAATGA+GGG + chr4_2:6614327-6614346 None:intergenic 15.0%
!! AAAGATAAATATGAAAAATG+AGG + chr4_2:6614328-6614347 None:intergenic 15.0%
!!! TATTACTTTTTATCATGTAT+AGG - chr4_2:6614427-6614446 Msa0585600:CDS 15.0%
!! TTAAATATTGTGATTATAAG+TGG - chr4_2:6615007-6615026 Msa0585600:intron 15.0%
!! TAAATATTGTGATTATAAGT+GGG - chr4_2:6615008-6615027 Msa0585600:intron 15.0%
!! AAGATAAATATGAAAAATGA+GGG + chr4_2:6614327-6614346 None:intergenic 15.0%
!! AAAGATAAATATGAAAAATG+AGG + chr4_2:6614328-6614347 None:intergenic 15.0%
!!! TATTACTTTTTATCATGTAT+AGG - chr4_2:6614427-6614446 Msa0585600:CDS 15.0%
!! TTAAATATTGTGATTATAAG+TGG - chr4_2:6615007-6615026 Msa0585600:intron 15.0%
!! TAAATATTGTGATTATAAGT+GGG - chr4_2:6615008-6615027 Msa0585600:intron 15.0%
!! AATTTGCATGCATTTATAAA+TGG - chr4_2:6614500-6614519 Msa0585600:intron 20.0%
!! ATTTGCATGCATTTATAAAT+GGG - chr4_2:6614501-6614520 Msa0585600:intron 20.0%
!!! ATTAAGCAATTTGTTTAAGA+AGG + chr4_2:6614616-6614635 None:intergenic 20.0%
!! AAATATTGTGATTATAAGTG+GGG - chr4_2:6615009-6615028 Msa0585600:intron 20.0%
!!! GTTTTATTTCTGAATGTTAA+TGG - chr4_2:6615137-6615156 Msa0585600:intron 20.0%
!! AATTTGCATGCATTTATAAA+TGG - chr4_2:6614500-6614519 Msa0585600:intron 20.0%
!! ATTTGCATGCATTTATAAAT+GGG - chr4_2:6614501-6614520 Msa0585600:intron 20.0%
!!! ATTAAGCAATTTGTTTAAGA+AGG + chr4_2:6614616-6614635 None:intergenic 20.0%
!! AAATATTGTGATTATAAGTG+GGG - chr4_2:6615009-6615028 Msa0585600:intron 20.0%
!!! GTTTTATTTCTGAATGTTAA+TGG - chr4_2:6615137-6615156 Msa0585600:intron 20.0%
!! GGAAGATATTTGAAGTATAT+CGG - chr4_2:6614184-6614203 Msa0585600:three_prime_UTR 25.0%
!! GAAGATATTTGAAGTATATC+GGG - chr4_2:6614185-6614204 Msa0585600:three_prime_UTR 25.0%
!!! ATGAAAAATGAGGGTTTTAA+AGG + chr4_2:6614318-6614337 None:intergenic 25.0%
! TATTCAATCTTCAGCAATAT+TGG + chr4_2:6615266-6615285 None:intergenic 25.0%
! TTAGCAGTCATTTATTTATG+TGG + chr4_2:6615292-6615311 None:intergenic 25.0%
! TAATCATCATCTTTCTTGTT+AGG + chr4_2:6615563-6615582 None:intergenic 25.0%
!!! GTTTTGTTTTGGGATTTTTT+GGG - chr4_2:6615624-6615643 Msa0585600:intron 25.0%
!!! TTTTGTTTTGGGATTTTTTG+GGG - chr4_2:6615625-6615644 Msa0585600:intron 25.0%
! TACTTGATCCTATATAATAG+AGG + chr4_2:6615661-6615680 None:intergenic 25.0%
!! GGAAGATATTTGAAGTATAT+CGG - chr4_2:6614184-6614203 Msa0585600:three_prime_UTR 25.0%
!! GAAGATATTTGAAGTATATC+GGG - chr4_2:6614185-6614204 Msa0585600:three_prime_UTR 25.0%
!!! ATGAAAAATGAGGGTTTTAA+AGG + chr4_2:6614318-6614337 None:intergenic 25.0%
! TATTCAATCTTCAGCAATAT+TGG + chr4_2:6615266-6615285 None:intergenic 25.0%
! TTAGCAGTCATTTATTTATG+TGG + chr4_2:6615292-6615311 None:intergenic 25.0%
! TAATCATCATCTTTCTTGTT+AGG + chr4_2:6615563-6615582 None:intergenic 25.0%
!!! GTTTTGTTTTGGGATTTTTT+GGG - chr4_2:6615624-6615643 Msa0585600:intron 25.0%
!!! TTTTGTTTTGGGATTTTTTG+GGG - chr4_2:6615625-6615644 Msa0585600:intron 25.0%
! TACTTGATCCTATATAATAG+AGG + chr4_2:6615661-6615680 None:intergenic 25.0%
!! TTAGTTTTCACATTTAGCAG+TGG - chr4_2:6614159-6614178 Msa0585600:three_prime_UTR 30.0%
! ATATTTGAAGTATATCGGGT+CGG - chr4_2:6614189-6614208 Msa0585600:three_prime_UTR 30.0%
TAATTCATGTATTATGGGTC+GGG + chr4_2:6614253-6614272 None:intergenic 30.0%
CTAATTCATGTATTATGGGT+CGG + chr4_2:6614254-6614273 None:intergenic 30.0%
CGACCTAATTCATGTATTAT+GGG + chr4_2:6614258-6614277 None:intergenic 30.0%
CCATAATACATGAATTAGGT+CGG - chr4_2:6614256-6614275 Msa0585600:CDS 30.0%
TAGGTAGAAAGAAGTTGTTT+CGG + chr4_2:6614472-6614491 None:intergenic 30.0%
AAACAACTTCTTTCTACCTA+GGG - chr4_2:6614472-6614491 Msa0585600:intron 30.0%
!! TTGTATTAATTTGCGTATCG+TGG + chr4_2:6614683-6614702 None:intergenic 30.0%
TAAGACTTATATGACAGAGT+TGG - chr4_2:6615096-6615115 Msa0585600:intron 30.0%
!!! GTATTTTTTCTCCATTTCCT+TGG + chr4_2:6615534-6615553 None:intergenic 30.0%
!!! GGTTTTGTTTTGGGATTTTT+TGG - chr4_2:6615623-6615642 Msa0585600:intron 30.0%
!!! TTTGTTTTGGGATTTTTTGG+GGG - chr4_2:6615626-6615645 Msa0585600:intron 30.0%
CGATTTGTCCTCTATTATAT+AGG - chr4_2:6615650-6615669 Msa0585600:CDS 30.0%
TAACTGACACGAATTAGTTA+AGG - chr4_2:6615688-6615707 Msa0585600:CDS 30.0%
!! TTAGTTTTCACATTTAGCAG+TGG - chr4_2:6614159-6614178 Msa0585600:three_prime_UTR 30.0%
! ATATTTGAAGTATATCGGGT+CGG - chr4_2:6614189-6614208 Msa0585600:three_prime_UTR 30.0%
TAATTCATGTATTATGGGTC+GGG + chr4_2:6614253-6614272 None:intergenic 30.0%
CTAATTCATGTATTATGGGT+CGG + chr4_2:6614254-6614273 None:intergenic 30.0%
CGACCTAATTCATGTATTAT+GGG + chr4_2:6614258-6614277 None:intergenic 30.0%
CCATAATACATGAATTAGGT+CGG - chr4_2:6614256-6614275 Msa0585600:CDS 30.0%
TAGGTAGAAAGAAGTTGTTT+CGG + chr4_2:6614472-6614491 None:intergenic 30.0%
AAACAACTTCTTTCTACCTA+GGG - chr4_2:6614472-6614491 Msa0585600:intron 30.0%
!! TTGTATTAATTTGCGTATCG+TGG + chr4_2:6614683-6614702 None:intergenic 30.0%
TAAGACTTATATGACAGAGT+TGG - chr4_2:6615096-6615115 Msa0585600:intron 30.0%
!!! GTATTTTTTCTCCATTTCCT+TGG + chr4_2:6615534-6615553 None:intergenic 30.0%
!!! GGTTTTGTTTTGGGATTTTT+TGG - chr4_2:6615623-6615642 Msa0585600:intron 30.0%
!!! TTTGTTTTGGGATTTTTTGG+GGG - chr4_2:6615626-6615645 Msa0585600:intron 30.0%
CGATTTGTCCTCTATTATAT+AGG - chr4_2:6615650-6615669 Msa0585600:CDS 30.0%
TAACTGACACGAATTAGTTA+AGG - chr4_2:6615688-6615707 Msa0585600:CDS 30.0%
! TTTTCACATTTAGCAGTGGA+TGG - chr4_2:6614163-6614182 Msa0585600:three_prime_UTR 35.0%
! TATTTGAAGTATATCGGGTC+GGG - chr4_2:6614190-6614209 Msa0585600:three_prime_UTR 35.0%
CGACCCATAATACATGAATT+AGG - chr4_2:6614252-6614271 Msa0585600:CDS 35.0%
CCGACCTAATTCATGTATTA+TGG + chr4_2:6614259-6614278 None:intergenic 35.0%
ATGAATTAGGTCGGATATGA+TGG - chr4_2:6614265-6614284 Msa0585600:CDS 35.0%
!! TCGGATATGATGGATCTATT+GGG - chr4_2:6614275-6614294 Msa0585600:CDS 35.0%
!!! GGTTTTAAAGGTCAGTTACA+GGG + chr4_2:6614306-6614325 None:intergenic 35.0%
GAAACAACTTCTTTCTACCT+AGG - chr4_2:6614471-6614490 Msa0585600:intron 35.0%
TTATAAATGGGAATCCCCAT+TGG - chr4_2:6614513-6614532 Msa0585600:intron 35.0%
TATAAATGGGAATCCCCATT+GGG - chr4_2:6614514-6614533 Msa0585600:intron 35.0%
TAAATAGGATAGTCCCAATG+GGG + chr4_2:6614530-6614549 None:intergenic 35.0%
CTAAATAGGATAGTCCCAAT+GGG + chr4_2:6614531-6614550 None:intergenic 35.0%
CATTGGGACTATCCTATTTA+GGG - chr4_2:6614530-6614549 Msa0585600:intron 35.0%
ATCCCATAAGCTAATCATAG+CGG - chr4_2:6614634-6614653 Msa0585600:intron 35.0%
!! TTCCGCTATGATTAGCTTAT+GGG + chr4_2:6614639-6614658 None:intergenic 35.0%
!! ATTCCGCTATGATTAGCTTA+TGG + chr4_2:6614640-6614659 None:intergenic 35.0%
TACAACGTGTTGTCAAATAG+CGG - chr4_2:6614698-6614717 Msa0585600:intron 35.0%
ACATTGTAGCATAGCAGAAT+TGG - chr4_2:6614737-6614756 Msa0585600:intron 35.0%
!! GTATTAACTTGCGTATCTGT+AGG + chr4_2:6614811-6614830 None:intergenic 35.0%
TAATACAACGTGCTGTCAAA+TGG - chr4_2:6614825-6614844 Msa0585600:intron 35.0%
AGACTCAGTATTGTCAATTG+CGG + chr4_2:6614916-6614935 None:intergenic 35.0%
AGTTATCACTAGACATCTGT+AGG + chr4_2:6614941-6614960 None:intergenic 35.0%
AACCGCAAAAGCTAACATTT+GGG + chr4_2:6615046-6615065 None:intergenic 35.0%
TTCTCAGTTACTGCAAATCA+AGG - chr4_2:6615375-6615394 Msa0585600:intron 35.0%
! AGGTTCTTCTCTTTTATAGC+AGG - chr4_2:6615428-6615447 Msa0585600:intron 35.0%
! GGTTCTTCTCTTTTATAGCA+GGG - chr4_2:6615429-6615448 Msa0585600:intron 35.0%
AAGCCGACAATTTCTTAGAT+TGG - chr4_2:6615507-6615526 Msa0585600:intron 35.0%
AGCCGACAATTTCTTAGATT+GGG - chr4_2:6615508-6615527 Msa0585600:intron 35.0%
CATCTTTCTTGTTAGGTTTG+TGG + chr4_2:6615556-6615575 None:intergenic 35.0%
AAAACAAAACCTGATTCCAC+TGG + chr4_2:6615614-6615633 None:intergenic 35.0%
!!! AGTGGAATCAGGTTTTGTTT+TGG - chr4_2:6615613-6615632 Msa0585600:intron 35.0%
!!! GTGGAATCAGGTTTTGTTTT+GGG - chr4_2:6615614-6615633 Msa0585600:intron 35.0%
!!! TTGTTTTGGGATTTTTTGGG+GGG - chr4_2:6615627-6615646 Msa0585600:intron 35.0%
! TTTTCACATTTAGCAGTGGA+TGG - chr4_2:6614163-6614182 Msa0585600:three_prime_UTR 35.0%
! TATTTGAAGTATATCGGGTC+GGG - chr4_2:6614190-6614209 Msa0585600:three_prime_UTR 35.0%
CGACCCATAATACATGAATT+AGG - chr4_2:6614252-6614271 Msa0585600:CDS 35.0%
CCGACCTAATTCATGTATTA+TGG + chr4_2:6614259-6614278 None:intergenic 35.0%
ATGAATTAGGTCGGATATGA+TGG - chr4_2:6614265-6614284 Msa0585600:CDS 35.0%
!! TCGGATATGATGGATCTATT+GGG - chr4_2:6614275-6614294 Msa0585600:CDS 35.0%
!!! GGTTTTAAAGGTCAGTTACA+GGG + chr4_2:6614306-6614325 None:intergenic 35.0%
GAAACAACTTCTTTCTACCT+AGG - chr4_2:6614471-6614490 Msa0585600:intron 35.0%
TTATAAATGGGAATCCCCAT+TGG - chr4_2:6614513-6614532 Msa0585600:intron 35.0%
TATAAATGGGAATCCCCATT+GGG - chr4_2:6614514-6614533 Msa0585600:intron 35.0%
TAAATAGGATAGTCCCAATG+GGG + chr4_2:6614530-6614549 None:intergenic 35.0%
CTAAATAGGATAGTCCCAAT+GGG + chr4_2:6614531-6614550 None:intergenic 35.0%
CATTGGGACTATCCTATTTA+GGG - chr4_2:6614530-6614549 Msa0585600:intron 35.0%
ATCCCATAAGCTAATCATAG+CGG - chr4_2:6614634-6614653 Msa0585600:intron 35.0%
!! TTCCGCTATGATTAGCTTAT+GGG + chr4_2:6614639-6614658 None:intergenic 35.0%
!! ATTCCGCTATGATTAGCTTA+TGG + chr4_2:6614640-6614659 None:intergenic 35.0%
TACAACGTGTTGTCAAATAG+CGG - chr4_2:6614698-6614717 Msa0585600:intron 35.0%
ACATTGTAGCATAGCAGAAT+TGG - chr4_2:6614737-6614756 Msa0585600:intron 35.0%
!! GTATTAACTTGCGTATCTGT+AGG + chr4_2:6614811-6614830 None:intergenic 35.0%
TAATACAACGTGCTGTCAAA+TGG - chr4_2:6614825-6614844 Msa0585600:intron 35.0%
AGACTCAGTATTGTCAATTG+CGG + chr4_2:6614916-6614935 None:intergenic 35.0%
AGTTATCACTAGACATCTGT+AGG + chr4_2:6614941-6614960 None:intergenic 35.0%
AACCGCAAAAGCTAACATTT+GGG + chr4_2:6615046-6615065 None:intergenic 35.0%
TTCTCAGTTACTGCAAATCA+AGG - chr4_2:6615375-6615394 Msa0585600:intron 35.0%
! AGGTTCTTCTCTTTTATAGC+AGG - chr4_2:6615428-6615447 Msa0585600:intron 35.0%
! GGTTCTTCTCTTTTATAGCA+GGG - chr4_2:6615429-6615448 Msa0585600:intron 35.0%
AAGCCGACAATTTCTTAGAT+TGG - chr4_2:6615507-6615526 Msa0585600:intron 35.0%
AGCCGACAATTTCTTAGATT+GGG - chr4_2:6615508-6615527 Msa0585600:intron 35.0%
CATCTTTCTTGTTAGGTTTG+TGG + chr4_2:6615556-6615575 None:intergenic 35.0%
AAAACAAAACCTGATTCCAC+TGG + chr4_2:6615614-6615633 None:intergenic 35.0%
!!! AGTGGAATCAGGTTTTGTTT+TGG - chr4_2:6615613-6615632 Msa0585600:intron 35.0%
!!! GTGGAATCAGGTTTTGTTTT+GGG - chr4_2:6615614-6615633 Msa0585600:intron 35.0%
!!! TTGTTTTGGGATTTTTTGGG+GGG - chr4_2:6615627-6615646 Msa0585600:intron 35.0%
! ATTTGAAGTATATCGGGTCG+GGG - chr4_2:6614191-6614210 Msa0585600:three_prime_UTR 40.0%
TGGATTTGGATTCACGAAAC+CGG - chr4_2:6614225-6614244 Msa0585600:three_prime_UTR 40.0%
!! GTCGGATATGATGGATCTAT+TGG - chr4_2:6614274-6614293 Msa0585600:CDS 40.0%
!!! GGGTTTTAAAGGTCAGTTAC+AGG + chr4_2:6614307-6614326 None:intergenic 40.0%
CCTAAATAGGATAGTCCCAA+TGG + chr4_2:6614532-6614551 None:intergenic 40.0%
CCATTGGGACTATCCTATTT+AGG - chr4_2:6614529-6614548 Msa0585600:intron 40.0%
! AGACTCGGTATTGTCAATTG+CGG + chr4_2:6614786-6614805 None:intergenic 40.0%
ATAACTTCACACTCTCGTCT+TGG - chr4_2:6614955-6614974 Msa0585600:intron 40.0%
!!! ACCCCAAATGTTAGCTTTTG+CGG - chr4_2:6615041-6615060 Msa0585600:intron 40.0%
ACCGCAAAAGCTAACATTTG+GGG + chr4_2:6615045-6615064 None:intergenic 40.0%
CAACCGCAAAAGCTAACATT+TGG + chr4_2:6615047-6615066 None:intergenic 40.0%
!! CACCTGTAATGGGTATAATC+AGG - chr4_2:6615405-6615424 Msa0585600:intron 40.0%
CTCCTGATTATACCCATTAC+AGG + chr4_2:6615410-6615429 None:intergenic 40.0%
!! CTGTAATGGGTATAATCAGG+AGG - chr4_2:6615408-6615427 Msa0585600:intron 40.0%
! CTCTTTTATAGCAGGGAATG+TGG - chr4_2:6615436-6615455 Msa0585600:intron 40.0%
TTTATAGCAGGGAATGTGGT+TGG - chr4_2:6615440-6615459 Msa0585600:intron 40.0%
TTATAGCAGGGAATGTGGTT+GGG - chr4_2:6615441-6615460 Msa0585600:intron 40.0%
GGCCCAATCTAAGAAATTGT+CGG + chr4_2:6615513-6615532 None:intergenic 40.0%
CAATTTCTTAGATTGGGCCA+AGG - chr4_2:6615514-6615533 Msa0585600:intron 40.0%
AACTTCTTTGACACTACCAG+TGG - chr4_2:6615595-6615614 Msa0585600:intron 40.0%
! ATTTGAAGTATATCGGGTCG+GGG - chr4_2:6614191-6614210 Msa0585600:three_prime_UTR 40.0%
TGGATTTGGATTCACGAAAC+CGG - chr4_2:6614225-6614244 Msa0585600:three_prime_UTR 40.0%
!! GTCGGATATGATGGATCTAT+TGG - chr4_2:6614274-6614293 Msa0585600:CDS 40.0%
!!! GGGTTTTAAAGGTCAGTTAC+AGG + chr4_2:6614307-6614326 None:intergenic 40.0%
CCTAAATAGGATAGTCCCAA+TGG + chr4_2:6614532-6614551 None:intergenic 40.0%
CCATTGGGACTATCCTATTT+AGG - chr4_2:6614529-6614548 Msa0585600:intron 40.0%
! AGACTCGGTATTGTCAATTG+CGG + chr4_2:6614786-6614805 None:intergenic 40.0%
ATAACTTCACACTCTCGTCT+TGG - chr4_2:6614955-6614974 Msa0585600:intron 40.0%
!!! ACCCCAAATGTTAGCTTTTG+CGG - chr4_2:6615041-6615060 Msa0585600:intron 40.0%
ACCGCAAAAGCTAACATTTG+GGG + chr4_2:6615045-6615064 None:intergenic 40.0%
CAACCGCAAAAGCTAACATT+TGG + chr4_2:6615047-6615066 None:intergenic 40.0%
!! CACCTGTAATGGGTATAATC+AGG - chr4_2:6615405-6615424 Msa0585600:intron 40.0%
CTCCTGATTATACCCATTAC+AGG + chr4_2:6615410-6615429 None:intergenic 40.0%
!! CTGTAATGGGTATAATCAGG+AGG - chr4_2:6615408-6615427 Msa0585600:intron 40.0%
! CTCTTTTATAGCAGGGAATG+TGG - chr4_2:6615436-6615455 Msa0585600:intron 40.0%
TTTATAGCAGGGAATGTGGT+TGG - chr4_2:6615440-6615459 Msa0585600:intron 40.0%
TTATAGCAGGGAATGTGGTT+GGG - chr4_2:6615441-6615460 Msa0585600:intron 40.0%
GGCCCAATCTAAGAAATTGT+CGG + chr4_2:6615513-6615532 None:intergenic 40.0%
CAATTTCTTAGATTGGGCCA+AGG - chr4_2:6615514-6615533 Msa0585600:intron 40.0%
AACTTCTTTGACACTACCAG+TGG - chr4_2:6615595-6615614 Msa0585600:intron 40.0%
AGAGAGACAACGGTGAGTTA+CGG - chr4_2:6614097-6614116 Msa0585600:three_prime_UTR 45.0%
! GAGAGTGCGAAGTTGGTTTA+CGG - chr4_2:6614124-6614143 Msa0585600:three_prime_UTR 45.0%
GGGTCATGGTTTCATGGATT+TGG - chr4_2:6614211-6614230 Msa0585600:three_prime_UTR 45.0%
ATGTATTATGGGTCGGGTAC+CGG + chr4_2:6614247-6614266 None:intergenic 45.0%
GCATGCAAATTGACATCCCT+AGG + chr4_2:6614491-6614510 None:intergenic 45.0%
TATTTAGGGATCCGCAAGCA+GGG - chr4_2:6614544-6614563 Msa0585600:intron 45.0%
! AAGCAGGGAATTTTTCCGCA+TGG - chr4_2:6614559-6614578 Msa0585600:intron 45.0%
! AGCAGGGAATTTTTCCGCAT+GGG - chr4_2:6614560-6614579 Msa0585600:intron 45.0%
GCGTATCTGTAGGATAGACT+CGG + chr4_2:6614801-6614820 None:intergenic 45.0%
TACAACGTGCTGTCAAATGG+CGG - chr4_2:6614828-6614847 Msa0585600:intron 45.0%
AAGGACTCAAGCACCTGTAA+TGG - chr4_2:6615394-6615413 Msa0585600:intron 45.0%
AGGACTCAAGCACCTGTAAT+GGG - chr4_2:6615395-6615414 Msa0585600:intron 45.0%
GAATGTGGTTGGGATCTATG+TGG - chr4_2:6615451-6615470 Msa0585600:intron 45.0%
! CCTGCTTGATGTTTGGAACT+TGG + chr4_2:6615487-6615506 None:intergenic 45.0%
CCAAGTTCCAAACATCAAGC+AGG - chr4_2:6615484-6615503 Msa0585600:intron 45.0%
CTTAGATTGGGCCAAGGAAA+TGG - chr4_2:6615520-6615539 Msa0585600:intron 45.0%
TTGACACTACCAGTGGAATC+AGG - chr4_2:6615602-6615621 Msa0585600:intron 45.0%
AGAGAGACAACGGTGAGTTA+CGG - chr4_2:6614097-6614116 Msa0585600:three_prime_UTR 45.0%
! GAGAGTGCGAAGTTGGTTTA+CGG - chr4_2:6614124-6614143 Msa0585600:three_prime_UTR 45.0%
GGGTCATGGTTTCATGGATT+TGG - chr4_2:6614211-6614230 Msa0585600:three_prime_UTR 45.0%
ATGTATTATGGGTCGGGTAC+CGG + chr4_2:6614247-6614266 None:intergenic 45.0%
GCATGCAAATTGACATCCCT+AGG + chr4_2:6614491-6614510 None:intergenic 45.0%
TATTTAGGGATCCGCAAGCA+GGG - chr4_2:6614544-6614563 Msa0585600:intron 45.0%
! AAGCAGGGAATTTTTCCGCA+TGG - chr4_2:6614559-6614578 Msa0585600:intron 45.0%
! AGCAGGGAATTTTTCCGCAT+GGG - chr4_2:6614560-6614579 Msa0585600:intron 45.0%
GCGTATCTGTAGGATAGACT+CGG + chr4_2:6614801-6614820 None:intergenic 45.0%
TACAACGTGCTGTCAAATGG+CGG - chr4_2:6614828-6614847 Msa0585600:intron 45.0%
AAGGACTCAAGCACCTGTAA+TGG - chr4_2:6615394-6615413 Msa0585600:intron 45.0%
AGGACTCAAGCACCTGTAAT+GGG - chr4_2:6615395-6615414 Msa0585600:intron 45.0%
GAATGTGGTTGGGATCTATG+TGG - chr4_2:6615451-6615470 Msa0585600:intron 45.0%
! CCTGCTTGATGTTTGGAACT+TGG + chr4_2:6615487-6615506 None:intergenic 45.0%
CCAAGTTCCAAACATCAAGC+AGG - chr4_2:6615484-6615503 Msa0585600:intron 45.0%
CTTAGATTGGGCCAAGGAAA+TGG - chr4_2:6615520-6615539 Msa0585600:intron 45.0%
TTGACACTACCAGTGGAATC+AGG - chr4_2:6615602-6615621 Msa0585600:intron 45.0%
TCGGAGATTATCACCGTTGC+CGG - chr4_2:6614068-6614087 Msa0585600:three_prime_UTR 50.0%
ATTATCACCGTTGCCGGAGA+CGG - chr4_2:6614074-6614093 Msa0585600:three_prime_UTR 50.0%
CTGCTTGCGGATCCCTAAAT+AGG + chr4_2:6614545-6614564 None:intergenic 50.0%
CTATTTAGGGATCCGCAAGC+AGG - chr4_2:6614543-6614562 Msa0585600:intron 50.0%
GCGGAAAAATTCCCTGCTTG+CGG + chr4_2:6614558-6614577 None:intergenic 50.0%
! GCAGGGAATTTTTCCGCATG+GGG - chr4_2:6614561-6614580 Msa0585600:intron 50.0%
! AATTTTTCCGCATGGGGACG+AGG - chr4_2:6614567-6614586 Msa0585600:intron 50.0%
ACACTCTCGTCTTGGACATG+AGG - chr4_2:6614963-6614982 Msa0585600:intron 50.0%
TCGGCTTCCTGCTTGATGTT+TGG + chr4_2:6615494-6615513 None:intergenic 50.0%
TCGGAGATTATCACCGTTGC+CGG - chr4_2:6614068-6614087 Msa0585600:three_prime_UTR 50.0%
ATTATCACCGTTGCCGGAGA+CGG - chr4_2:6614074-6614093 Msa0585600:three_prime_UTR 50.0%
CTGCTTGCGGATCCCTAAAT+AGG + chr4_2:6614545-6614564 None:intergenic 50.0%
CTATTTAGGGATCCGCAAGC+AGG - chr4_2:6614543-6614562 Msa0585600:intron 50.0%
GCGGAAAAATTCCCTGCTTG+CGG + chr4_2:6614558-6614577 None:intergenic 50.0%
! GCAGGGAATTTTTCCGCATG+GGG - chr4_2:6614561-6614580 Msa0585600:intron 50.0%
! AATTTTTCCGCATGGGGACG+AGG - chr4_2:6614567-6614586 Msa0585600:intron 50.0%
ACACTCTCGTCTTGGACATG+AGG - chr4_2:6614963-6614982 Msa0585600:intron 50.0%
TCGGCTTCCTGCTTGATGTT+TGG + chr4_2:6615494-6615513 None:intergenic 50.0%
AGTATATCGGGTCGGGGTCA+TGG - chr4_2:6614197-6614216 Msa0585600:three_prime_UTR 55.0%
AGTATATCGGGTCGGGGTCA+TGG - chr4_2:6614197-6614216 Msa0585600:three_prime_UTR 55.0%
TCTCTCTCCGTCTCCGGCAA+CGG + chr4_2:6614084-6614103 None:intergenic 60.0%
CCGTTGTCTCTCTCCGTCTC+CGG + chr4_2:6614090-6614109 None:intergenic 60.0%
CCGGAGACGGAGAGAGACAA+CGG - chr4_2:6614087-6614106 Msa0585600:three_prime_UTR 60.0%
! CGGCAGAGAGAGTGCGAAGT+TGG - chr4_2:6614117-6614136 Msa0585600:three_prime_UTR 60.0%
GGGTCGGGGTCATGGTTTCA+TGG - chr4_2:6614205-6614224 Msa0585600:three_prime_UTR 60.0%
TCCACATCCTCGTCCCCATG+CGG + chr4_2:6614577-6614596 None:intergenic 60.0%
TCTCTCTCCGTCTCCGGCAA+CGG + chr4_2:6614084-6614103 None:intergenic 60.0%
CCGTTGTCTCTCTCCGTCTC+CGG + chr4_2:6614090-6614109 None:intergenic 60.0%
CCGGAGACGGAGAGAGACAA+CGG - chr4_2:6614087-6614106 Msa0585600:three_prime_UTR 60.0%
! CGGCAGAGAGAGTGCGAAGT+TGG - chr4_2:6614117-6614136 Msa0585600:three_prime_UTR 60.0%
GGGTCGGGGTCATGGTTTCA+TGG - chr4_2:6614205-6614224 Msa0585600:three_prime_UTR 60.0%
TCCACATCCTCGTCCCCATG+CGG + chr4_2:6614577-6614596 None:intergenic 60.0%
TCCGCATGGGGACGAGGATG+TGG - chr4_2:6614573-6614592 Msa0585600:intron 65.0%
TCCGCATGGGGACGAGGATG+TGG - chr4_2:6614573-6614592 Msa0585600:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 6614063 6615765 6614063 ID=Msa0585600;Name=Msa0585600
chr4_2 mRNA 6614063 6615765 6614063 ID=Msa0585600-mRNA-1;Parent=Msa0585600;Name=Msa0585600-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0|0.5|0.5|0|0|2|182|115
chr4_2 exon 6614063 6614457 6614063 ID=Msa0585600-mRNA-1:exon:1620;Parent=Msa0585600-mRNA-1
chr4_2 exon 6615631 6615765 6615631 ID=Msa0585600-mRNA-1:exon:1619;Parent=Msa0585600-mRNA-1
chr4_2 CDS 6615631 6615765 6615631 ID=Msa0585600-mRNA-1:cds;Parent=Msa0585600-mRNA-1
chr4_2 CDS 6614245 6614457 6614245 ID=Msa0585600-mRNA-1:cds;Parent=Msa0585600-mRNA-1
chr4_2 three_prime_UTR 6614063 6614244 6614063 ID=Msa0585600-mRNA-1:three_prime_utr;Parent=Msa0585600-mRNA-1
Gene Sequence

>Msa0585600

ATGTATCGGAGATTATCACCGTTGCCGGAGACGGAGAGAGACAACGGTGAGTTACGGCAGAGAGAGTGCGAAGTTGGTTTACGGCTGCAACTAGTGTTAGTTTTCACATTTAGCAGTGGATGGAAGATATTTGAAATATTTCTCAGTTACTGCAAATCAAGGACTCAAGCACCTGTAATGGGTATAATCAGGAGGTTCTTCTCTTTTATAGCAGGGAATGTGGTTGGGATCTATGTGGCTCAGAATTACCAAGTTCCAAACATCAAGCAGGAAGCCGACAATTTCTTAGATTGGGCCAAGGAAATGGAGAAAAAATACCACAAACCTAACAAGAAAGATGATGATTAG
Protein sequence

>Msa0585600

MYRRLSPLPETERDNGELRQRECEVGLRLQLVLVFTFSSGWKIFEIFLSYCKSRTQAPVMGIIRRFFSFIAGNVVGIYVAQNYQVPNIKQEADNFLDWAKEMEKKYHKPNKKDDD*