Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0607210 | PNY16827.1 | 79.221 | 77 | 15 | 1 | 10 | 85 | 355 | 431 | 4.48e-34 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0607210 | sp|Q84P54|GATP1_SOLLC | 82.051 | 39 | 7 | 0 | 17 | 55 | 454 | 492 | 1.23e-17 | 79.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0056470 | Msa0607210 | 0.805665 | 1.196114e-49 | -8.615850e-47 |
| Msa0086130 | Msa0607210 | 0.802378 | 5.772041e-49 | -8.615850e-47 |
| Msa0607210 | Msa1165940 | 0.809232 | 2.094762e-50 | -8.615850e-47 |
| Msa0607210 | Msa1451560 | 0.822584 | 2.190710e-53 | -8.615850e-47 |
| Msa0234050 | Msa0607210 | 0.800529 | 1.380901e-48 | -8.615850e-47 |
| Msa0419310 | Msa0607210 | 0.802025 | 6.821749e-49 | -8.615850e-47 |
| Msa0469990 | Msa0607210 | 0.830237 | 3.288615e-55 | -8.615850e-47 |
| Msa0548240 | Msa0607210 | 0.801813 | 7.542139e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0607210 | MtrunA17_Chr4g0038831 | 94.872 | 39 | 2 | 0 | 17 | 55 | 451 | 489 | 5.23e-20 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0607210 | AT3G22200.2 | 79.487 | 39 | 8 | 0 | 17 | 55 | 450 | 488 | 4.19e-17 | 75.1 |
| Msa0607210 | AT3G22200.1 | 79.487 | 39 | 8 | 0 | 17 | 55 | 441 | 479 | 4.22e-17 | 75.1 |
Find 19 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTAGTTAATCAGCATTTA+TGG | 0.143292 | 4_2:-53976139 | Msa0607210:intron |
| ACAGGAGTAGGTGCCTATTT+TGG | 0.230656 | 4_2:-53976816 | Msa0607210:CDS |
| TCTTCAGGTGATATAATAAA+TGG | 0.277964 | 4_2:+53976729 | None:intergenic |
| GGGATGCTAGTTCGTGTAGC+TGG | 0.294169 | 4_2:-53976774 | Msa0607210:CDS |
| CTCGCACTGTGCTCCAAAAT+AGG | 0.327909 | 4_2:+53976803 | None:intergenic |
| GTTACCTCGTCAACTTCTTC+AGG | 0.342007 | 4_2:+53976714 | None:intergenic |
| CCTAGTTAATCAGCATTTAT+GGG | 0.359037 | 4_2:-53976138 | Msa0607210:intron |
| AACACTATGATGTACATTAC+AGG | 0.373821 | 4_2:-53976834 | Msa0607210:CDS |
| GGATGCTAGTTCGTGTAGCT+GGG | 0.441540 | 4_2:-53976773 | Msa0607210:CDS |
| ATCATAGTGTTAGAACAAAC+TGG | 0.453916 | 4_2:+53976846 | None:intergenic |
| TGGGAAAGCTTTAAGAGAGA+CGG | 0.523625 | 4_2:-53976119 | Msa0607210:CDS |
| GGAGCACAGTGCGAGAAGCG+TGG | 0.580915 | 4_2:-53976795 | Msa0607210:CDS |
| GAGCACAGTGCGAGAAGCGT+GGG | 0.583956 | 4_2:-53976794 | Msa0607210:CDS |
| ATGATGTACATTACAGGAGT+AGG | 0.598372 | 4_2:-53976828 | Msa0607210:CDS |
| AAAATCTCCTCTTCACACAA+AGG | 0.612573 | 4_2:+53976019 | None:intergenic |
| TCAGGTGATATAATAAATGG+TGG | 0.616932 | 4_2:+53976732 | None:intergenic |
| AGTCTCAACGCAAGTAGCTG+AGG | 0.624074 | 4_2:-53976075 | Msa0607210:CDS |
| GATGCTAGTTCGTGTAGCTG+GGG | 0.670876 | 4_2:-53976772 | Msa0607210:CDS |
| ATCACCTGAAGAAGTTGACG+AGG | 0.716638 | 4_2:-53976718 | Msa0607210:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAATTATTGAATATGAG+CGG | - | chr4_2:53976263-53976282 | Msa0607210:intron | 15.0% |
| !! | ATTAAATTATTGAATATGAG+CGG | - | chr4_2:53976263-53976282 | Msa0607210:intron | 15.0% |
| !!! | TAAATACTCTTTTATTTGAG+TGG | - | chr4_2:53976414-53976433 | Msa0607210:intron | 20.0% |
| !! | AATTGTACATACAAAGTAAT+TGG | - | chr4_2:53976449-53976468 | Msa0607210:intron | 20.0% |
| !! | AAGTAATTGGAAGAAAAAAT+TGG | - | chr4_2:53976462-53976481 | Msa0607210:intron | 20.0% |
| !! | ACTAACTAGTCAATTAATTT+GGG | + | chr4_2:53976573-53976592 | None:intergenic | 20.0% |
| !! | TACTAACTAGTCAATTAATT+TGG | + | chr4_2:53976574-53976593 | None:intergenic | 20.0% |
| !!! | TAAATACTCTTTTATTTGAG+TGG | - | chr4_2:53976414-53976433 | Msa0607210:intron | 20.0% |
| !! | AATTGTACATACAAAGTAAT+TGG | - | chr4_2:53976449-53976468 | Msa0607210:intron | 20.0% |
| !! | AAGTAATTGGAAGAAAAAAT+TGG | - | chr4_2:53976462-53976481 | Msa0607210:intron | 20.0% |
| !! | ACTAACTAGTCAATTAATTT+GGG | + | chr4_2:53976573-53976592 | None:intergenic | 20.0% |
| !! | TACTAACTAGTCAATTAATT+TGG | + | chr4_2:53976574-53976593 | None:intergenic | 20.0% |
| ! | TCTTCAGGTGATATAATAAA+TGG | + | chr4_2:53976166-53976185 | None:intergenic | 25.0% |
| ! | CTTTCTTCAATTTAATGAAG+TGG | + | chr4_2:53976228-53976247 | None:intergenic | 25.0% |
| !! | GTTGCAAAATTTTCAAATGA+AGG | - | chr4_2:53976379-53976398 | Msa0607210:intron | 25.0% |
| !! | TAATTGACTAGTTAGTATGA+TGG | - | chr4_2:53976577-53976596 | Msa0607210:intron | 25.0% |
| ! | CTGAATAACAGTATAAATGA+GGG | + | chr4_2:53976659-53976678 | None:intergenic | 25.0% |
| ! | ACTGAATAACAGTATAAATG+AGG | + | chr4_2:53976660-53976679 | None:intergenic | 25.0% |
| !! | GATACTGCAGATTTTAAATT+TGG | - | chr4_2:53976697-53976716 | Msa0607210:intron | 25.0% |
| ! | TCTTCAGGTGATATAATAAA+TGG | + | chr4_2:53976166-53976185 | None:intergenic | 25.0% |
| ! | CTTTCTTCAATTTAATGAAG+TGG | + | chr4_2:53976228-53976247 | None:intergenic | 25.0% |
| !! | GTTGCAAAATTTTCAAATGA+AGG | - | chr4_2:53976379-53976398 | Msa0607210:intron | 25.0% |
| !! | TAATTGACTAGTTAGTATGA+TGG | - | chr4_2:53976577-53976596 | Msa0607210:intron | 25.0% |
| ! | CTGAATAACAGTATAAATGA+GGG | + | chr4_2:53976659-53976678 | None:intergenic | 25.0% |
| ! | ACTGAATAACAGTATAAATG+AGG | + | chr4_2:53976660-53976679 | None:intergenic | 25.0% |
| !! | GATACTGCAGATTTTAAATT+TGG | - | chr4_2:53976697-53976716 | Msa0607210:intron | 25.0% |
| ATCATAGTGTTAGAACAAAC+TGG | + | chr4_2:53976049-53976068 | None:intergenic | 30.0% | |
| AACACTATGATGTACATTAC+AGG | - | chr4_2:53976058-53976077 | Msa0607210:CDS | 30.0% | |
| TCAGGTGATATAATAAATGG+TGG | + | chr4_2:53976163-53976182 | None:intergenic | 30.0% | |
| ! | TGCTGATTAACTAGGAAAAA+AGG | + | chr4_2:53976749-53976768 | None:intergenic | 30.0% |
| TCCTAGTTAATCAGCATTTA+TGG | - | chr4_2:53976753-53976772 | Msa0607210:CDS | 30.0% | |
| CCTAGTTAATCAGCATTTAT+GGG | - | chr4_2:53976754-53976773 | Msa0607210:CDS | 30.0% | |
| ATCATAGTGTTAGAACAAAC+TGG | + | chr4_2:53976049-53976068 | None:intergenic | 30.0% | |
| AACACTATGATGTACATTAC+AGG | - | chr4_2:53976058-53976077 | Msa0607210:CDS | 30.0% | |
| TCAGGTGATATAATAAATGG+TGG | + | chr4_2:53976163-53976182 | None:intergenic | 30.0% | |
| ! | TGCTGATTAACTAGGAAAAA+AGG | + | chr4_2:53976749-53976768 | None:intergenic | 30.0% |
| TCCTAGTTAATCAGCATTTA+TGG | - | chr4_2:53976753-53976772 | Msa0607210:CDS | 30.0% | |
| CCTAGTTAATCAGCATTTAT+GGG | - | chr4_2:53976754-53976773 | Msa0607210:CDS | 30.0% | |
| ATGATGTACATTACAGGAGT+AGG | - | chr4_2:53976064-53976083 | Msa0607210:CDS | 35.0% | |
| TCAATCATCCTAGGTTCTTA+AGG | - | chr4_2:53976309-53976328 | Msa0607210:intron | 35.0% | |
| CAATCATCCTAGGTTCTTAA+GGG | - | chr4_2:53976310-53976329 | Msa0607210:intron | 35.0% | |
| TGTTTATGTTATGCTTGCAG+AGG | + | chr4_2:53976618-53976637 | None:intergenic | 35.0% | |
| ! | CCCATAAATGCTGATTAACT+AGG | + | chr4_2:53976757-53976776 | None:intergenic | 35.0% |
| ATGATGTACATTACAGGAGT+AGG | - | chr4_2:53976064-53976083 | Msa0607210:CDS | 35.0% | |
| TCAATCATCCTAGGTTCTTA+AGG | - | chr4_2:53976309-53976328 | Msa0607210:intron | 35.0% | |
| CAATCATCCTAGGTTCTTAA+GGG | - | chr4_2:53976310-53976329 | Msa0607210:intron | 35.0% | |
| TGTTTATGTTATGCTTGCAG+AGG | + | chr4_2:53976618-53976637 | None:intergenic | 35.0% | |
| ! | CCCATAAATGCTGATTAACT+AGG | + | chr4_2:53976757-53976776 | None:intergenic | 35.0% |
| CTCTTCTGTTCAATCATCCT+AGG | - | chr4_2:53976300-53976319 | Msa0607210:intron | 40.0% | |
| GACTTGTATGTTTGGATGTG+TGG | + | chr4_2:53976488-53976507 | None:intergenic | 40.0% | |
| !! | GGCTGATTGACTTGTATGTT+TGG | + | chr4_2:53976496-53976515 | None:intergenic | 40.0% |
| !! | TGGGAAAGCTTTAAGAGAGA+CGG | - | chr4_2:53976773-53976792 | Msa0607210:CDS | 40.0% |
| CTCTTCTGTTCAATCATCCT+AGG | - | chr4_2:53976300-53976319 | Msa0607210:intron | 40.0% | |
| GACTTGTATGTTTGGATGTG+TGG | + | chr4_2:53976488-53976507 | None:intergenic | 40.0% | |
| !! | GGCTGATTGACTTGTATGTT+TGG | + | chr4_2:53976496-53976515 | None:intergenic | 40.0% |
| !! | TGGGAAAGCTTTAAGAGAGA+CGG | - | chr4_2:53976773-53976792 | Msa0607210:CDS | 40.0% |
| ! | ACAGGAGTAGGTGCCTATTT+TGG | - | chr4_2:53976076-53976095 | Msa0607210:CDS | 45.0% |
| ATCACCTGAAGAAGTTGACG+AGG | - | chr4_2:53976174-53976193 | Msa0607210:intron | 45.0% | |
| GTTACCTCGTCAACTTCTTC+AGG | + | chr4_2:53976181-53976200 | None:intergenic | 45.0% | |
| ATGAGGTCCCTTAAGAACCT+AGG | + | chr4_2:53976320-53976339 | None:intergenic | 45.0% | |
| AGGTTCTTAAGGGACCTCAT+TGG | - | chr4_2:53976320-53976339 | Msa0607210:intron | 45.0% | |
| TTCTTAAGGGACCTCATTGG+TGG | - | chr4_2:53976323-53976342 | Msa0607210:intron | 45.0% | |
| TCTTAAGGGACCTCATTGGT+GGG | - | chr4_2:53976324-53976343 | Msa0607210:intron | 45.0% | |
| GAAACAAGAGTTCCCCTGAT+GGG | + | chr4_2:53976529-53976548 | None:intergenic | 45.0% | |
| TGAAACAAGAGTTCCCCTGA+TGG | + | chr4_2:53976530-53976549 | None:intergenic | 45.0% | |
| ! | ACAGGAGTAGGTGCCTATTT+TGG | - | chr4_2:53976076-53976095 | Msa0607210:CDS | 45.0% |
| ATCACCTGAAGAAGTTGACG+AGG | - | chr4_2:53976174-53976193 | Msa0607210:intron | 45.0% | |
| GTTACCTCGTCAACTTCTTC+AGG | + | chr4_2:53976181-53976200 | None:intergenic | 45.0% | |
| ATGAGGTCCCTTAAGAACCT+AGG | + | chr4_2:53976320-53976339 | None:intergenic | 45.0% | |
| AGGTTCTTAAGGGACCTCAT+TGG | - | chr4_2:53976320-53976339 | Msa0607210:intron | 45.0% | |
| TTCTTAAGGGACCTCATTGG+TGG | - | chr4_2:53976323-53976342 | Msa0607210:intron | 45.0% | |
| TCTTAAGGGACCTCATTGGT+GGG | - | chr4_2:53976324-53976343 | Msa0607210:intron | 45.0% | |
| GAAACAAGAGTTCCCCTGAT+GGG | + | chr4_2:53976529-53976548 | None:intergenic | 45.0% | |
| TGAAACAAGAGTTCCCCTGA+TGG | + | chr4_2:53976530-53976549 | None:intergenic | 45.0% | |
| CTCGCACTGTGCTCCAAAAT+AGG | + | chr4_2:53976092-53976111 | None:intergenic | 50.0% | |
| GGATGCTAGTTCGTGTAGCT+GGG | - | chr4_2:53976119-53976138 | Msa0607210:CDS | 50.0% | |
| GATGCTAGTTCGTGTAGCTG+GGG | - | chr4_2:53976120-53976139 | Msa0607210:CDS | 50.0% | |
| CTTAAGGGACCTCATTGGTG+GGG | - | chr4_2:53976325-53976344 | Msa0607210:intron | 50.0% | |
| TATGCATGTCCCCACCAATG+AGG | + | chr4_2:53976337-53976356 | None:intergenic | 50.0% | |
| AGCCTATGCACATTCCCATC+AGG | - | chr4_2:53976512-53976531 | Msa0607210:intron | 50.0% | |
| GCCTATGCACATTCCCATCA+GGG | - | chr4_2:53976513-53976532 | Msa0607210:intron | 50.0% | |
| CCTATGCACATTCCCATCAG+GGG | - | chr4_2:53976514-53976533 | Msa0607210:intron | 50.0% | |
| AGTCTCAACGCAAGTAGCTG+AGG | - | chr4_2:53976817-53976836 | Msa0607210:CDS | 50.0% | |
| CTCGCACTGTGCTCCAAAAT+AGG | + | chr4_2:53976092-53976111 | None:intergenic | 50.0% | |
| GGATGCTAGTTCGTGTAGCT+GGG | - | chr4_2:53976119-53976138 | Msa0607210:CDS | 50.0% | |
| GATGCTAGTTCGTGTAGCTG+GGG | - | chr4_2:53976120-53976139 | Msa0607210:CDS | 50.0% | |
| CTTAAGGGACCTCATTGGTG+GGG | - | chr4_2:53976325-53976344 | Msa0607210:intron | 50.0% | |
| TATGCATGTCCCCACCAATG+AGG | + | chr4_2:53976337-53976356 | None:intergenic | 50.0% | |
| AGCCTATGCACATTCCCATC+AGG | - | chr4_2:53976512-53976531 | Msa0607210:intron | 50.0% | |
| GCCTATGCACATTCCCATCA+GGG | - | chr4_2:53976513-53976532 | Msa0607210:intron | 50.0% | |
| CCTATGCACATTCCCATCAG+GGG | - | chr4_2:53976514-53976533 | Msa0607210:intron | 50.0% | |
| AGTCTCAACGCAAGTAGCTG+AGG | - | chr4_2:53976817-53976836 | Msa0607210:CDS | 50.0% | |
| GGGATGCTAGTTCGTGTAGC+TGG | - | chr4_2:53976118-53976137 | Msa0607210:CDS | 55.0% | |
| CCCCTGATGGGAATGTGCAT+AGG | + | chr4_2:53976517-53976536 | None:intergenic | 55.0% | |
| GGGATGCTAGTTCGTGTAGC+TGG | - | chr4_2:53976118-53976137 | Msa0607210:CDS | 55.0% | |
| CCCCTGATGGGAATGTGCAT+AGG | + | chr4_2:53976517-53976536 | None:intergenic | 55.0% | |
| ! | GAGCACAGTGCGAGAAGCGT+GGG | - | chr4_2:53976098-53976117 | Msa0607210:CDS | 60.0% |
| ! | GAGCACAGTGCGAGAAGCGT+GGG | - | chr4_2:53976098-53976117 | Msa0607210:CDS | 60.0% |
| GGAGCACAGTGCGAGAAGCG+TGG | - | chr4_2:53976097-53976116 | Msa0607210:CDS | 65.0% | |
| GGAGCACAGTGCGAGAAGCG+TGG | - | chr4_2:53976097-53976116 | Msa0607210:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 53976031 | 53976883 | 53976031 | ID=Msa0607210;Name=Msa0607210 |
| chr4_2 | mRNA | 53976031 | 53976883 | 53976031 | ID=Msa0607210-mRNA-1;Parent=Msa0607210;Name=Msa0607210-mRNA-1;_AED=0.54;_eAED=0.77;_QI=0|0|0|1|1|1|2|0|93 |
| chr4_2 | exon | 53976719 | 53976883 | 53976719 | ID=Msa0607210-mRNA-1:exon:13436;Parent=Msa0607210-mRNA-1 |
| chr4_2 | exon | 53976031 | 53976147 | 53976031 | ID=Msa0607210-mRNA-1:exon:13435;Parent=Msa0607210-mRNA-1 |
| chr4_2 | CDS | 53976719 | 53976883 | 53976719 | ID=Msa0607210-mRNA-1:cds;Parent=Msa0607210-mRNA-1 |
| chr4_2 | CDS | 53976031 | 53976147 | 53976031 | ID=Msa0607210-mRNA-1:cds;Parent=Msa0607210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
