AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0419310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0419310 MtrunA17_Chr3g0116981 98.726 157 2 0 1 157 196 352 4.76e-116 332
Msa0419310 MtrunA17_Chr6g0449241 71.975 157 44 0 1 157 196 352 3.16e-84 251
Msa0419310 MtrunA17_Chr7g0244621 68.367 98 30 1 1 97 215 312 3.11e-42 144
Msa0419310 MtrunA17_Chr5g0403721 39.583 96 57 1 3 98 155 249 1.53e-20 86.3
Msa0419310 MtrunA17_Chr3g0143531 43.617 94 52 1 11 103 193 286 1.26e-19 82.8
Msa0419310 MtrunA17_Chr3g0090261 45.679 81 37 2 15 91 259 336 2.04e-17 77.8
Msa0419310 MtrunA17_Chr3g0117731 41.975 81 46 1 12 91 231 311 4.99e-17 76.6
Msa0419310 MtrunA17_Chr4g0059121 44.706 85 45 2 11 94 232 315 8.58e-17 75.9
Msa0419310 MtrunA17_Chr4g0047191 36.471 85 53 1 11 94 219 303 1.01e-15 72.8
Msa0419310 MtrunA17_Chr4g0047211 36.471 85 53 1 11 94 214 298 1.03e-15 72.8
Msa0419310 MtrunA17_Chr4g0047201 35.294 85 54 1 11 94 375 459 1.19e-15 73.2
Msa0419310 MtrunA17_Chr4g0075541 36.937 111 63 2 11 117 260 367 2.76e-15 71.6
Msa0419310 MtrunA17_Chr2g0282321 35.294 85 54 1 11 94 336 420 3.35e-15 71.6
Msa0419310 MtrunA17_Chr1g0211611 40.000 85 44 2 11 91 469 550 6.15e-15 71.2
Msa0419310 MtrunA17_Chr1g0151541 36.264 91 51 2 16 102 839 926 6.72e-14 68.2
Msa0419310 MtrunA17_Chr5g0406251 37.647 85 46 2 11 91 463 544 9.82e-14 67.8
Msa0419310 MtrunA17_Chr1g0191171 40.000 80 41 2 16 91 865 941 3.15e-13 66.2
Msa0419310 MtrunA17_Chr5g0427651 36.264 91 54 2 16 105 824 911 1.18e-12 64.7
Msa0419310 MtrunA17_Chr6g0482711 36.364 77 48 1 16 91 836 912 8.74e-12 62.0
Msa0419310 MtrunA17_Chr2g0294351 35.000 80 45 2 16 91 642 718 5.11e-11 59.7
Msa0419310 MtrunA17_Chr1g0205071 36.250 80 50 1 13 91 274 353 6.59e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0419310 AT5G50180.1 75.159 157 39 0 1 157 190 346 1.02e-84 252
Msa0419310 AT5G40540.3 68.987 158 42 3 1 152 99 255 4.86e-72 217
Msa0419310 AT5G40540.2 68.987 158 42 3 1 152 120 276 8.09e-72 217
Msa0419310 AT5G40540.1 68.987 158 42 3 1 152 196 352 7.75e-71 217
Msa0419310 AT3G27560.1 66.667 162 42 3 1 152 196 355 1.55e-68 211
Msa0419310 AT5G01850.1 69.565 92 27 1 1 91 188 279 4.78e-39 135
Msa0419310 AT5G01850.2 69.565 92 27 1 1 91 212 303 9.13e-39 135
Msa0419310 AT5G66710.1 51.908 131 57 3 1 131 241 365 1.11e-33 122
Msa0419310 AT5G40540.4 92.453 53 4 0 1 53 196 248 2.10e-29 108
Msa0419310 AT3G50730.1 42.424 99 57 0 1 99 206 304 1.84e-18 80.9
Msa0419310 AT3G50720.1 44.444 81 44 1 7 87 224 303 7.02e-18 79.3
Msa0419310 AT2G24360.1 42.169 83 45 2 11 91 303 384 5.75e-17 77.0
Msa0419310 AT4G31170.7 44.578 83 43 2 11 91 304 385 5.94e-17 77.0
Msa0419310 AT4G31170.6 44.578 83 43 2 11 91 304 385 5.94e-17 77.0
Msa0419310 AT4G31170.5 44.578 83 43 2 11 91 304 385 5.94e-17 77.0
Msa0419310 AT4G31170.1 44.578 83 43 2 11 91 304 385 5.94e-17 77.0
Msa0419310 AT4G31170.2 44.578 83 43 2 11 91 304 385 5.94e-17 77.0
Msa0419310 AT4G31170.3 44.578 83 43 2 11 91 304 385 5.94e-17 77.0
Msa0419310 AT4G31170.8 44.578 83 43 2 11 91 304 385 5.94e-17 77.0
Msa0419310 AT4G35780.1 40.860 93 48 2 11 99 460 549 8.03e-17 77.0
Msa0419310 AT1G62400.1 42.268 97 49 2 11 103 259 352 2.39e-16 75.1
Msa0419310 AT4G38470.2 40.860 93 48 2 11 99 458 547 4.71e-16 74.7
Msa0419310 AT4G38470.1 42.353 85 42 2 11 91 458 539 5.38e-16 74.3
Msa0419310 AT2G17700.1 38.710 93 50 2 11 99 454 543 3.20e-15 72.4
Msa0419310 AT5G58950.1 32.584 89 59 1 11 98 382 470 1.03e-14 70.9
Msa0419310 AT5G11850.1 38.961 77 46 1 16 91 785 861 7.25e-14 68.6
Msa0419310 AT1G08720.1 38.462 91 47 3 16 100 845 932 1.49e-13 67.4
Msa0419310 AT3G06640.2 36.782 87 50 3 16 98 622 707 8.48e-12 62.4
Msa0419310 AT3G06640.3 36.782 87 50 3 16 98 622 707 8.48e-12 62.4
Msa0419310 AT3G06640.1 36.782 87 50 3 16 98 622 707 9.73e-12 62.4
Msa0419310 AT3G06630.2 35.897 78 47 2 16 91 610 686 5.29e-11 60.1

Find 50 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTATAAGCATCGACTTTA+TGG 0.165014 3_2:+67234107 None:intergenic
ATAGCTTTGCAATCGTATTT+TGG 0.171547 3_2:-67234090 Msa0419310:CDS
CCCTGGTACGAGCTCCTTAA+TGG 0.231520 3_2:-67233699 Msa0419310:CDS
AAACGGTGTAAAGATAATTT+AGG 0.320150 3_2:+67233789 None:intergenic
GAGCTCGTACCAGGGGATTC+TGG 0.324358 3_2:+67233707 None:intergenic
AAAGATAATTTAGGAGCATT+TGG 0.328831 3_2:+67233798 None:intergenic
TGCATATGCTGCAGCCTTCA+AGG 0.343559 3_2:-67234017 Msa0419310:intron
GCCATTAAGGAGCTCGTACC+AGG 0.351285 3_2:+67233698 None:intergenic
ATGATCTTAGATTACCTTGA+AGG 0.387995 3_2:+67234003 None:intergenic
AGGATTACAGCCAATTCTTC+GGG 0.389095 3_2:+67233875 None:intergenic
AAGGATTACAGCCAATTCTT+CGG 0.394234 3_2:+67233874 None:intergenic
CTGCAGCATATGCAGCTTGA+AGG 0.405495 3_2:+67234026 None:intergenic
TCAGAAGTGAATATTCTTGA+AGG 0.408905 3_2:+67233746 None:intergenic
AATATTCTTGAAGGAATTGC+AGG 0.414396 3_2:+67233755 None:intergenic
GGATCATCTTCCCGAAGAAT+TGG 0.421850 3_2:-67233885 Msa0419310:CDS
CAGGTCTTTAAAGTACAAAA+TGG 0.434675 3_2:-67233579 Msa0419310:three_prime_UTR
AGTGTTATTAACTCTTGAAC+AGG 0.442073 3_2:-67233598 Msa0419310:exon
TTGGATTATCTGTGTGAAGT+TGG 0.454844 3_2:+67233817 None:intergenic
TTGATTTCGTCCTTTACTCT+AGG 0.464060 3_2:+67233650 None:intergenic
TGGATTATCTGTGTGAAGTT+GGG 0.483999 3_2:+67233818 None:intergenic
AAACAGCAAAGGAAACCATT+AGG 0.491144 3_2:+67233626 None:intergenic
ACATAGAATCTTGTCTAAAG+AGG 0.497706 3_2:-67233539 Msa0419310:three_prime_UTR
CCTCTTGCCAGCATGAAGTA+AGG 0.498570 3_2:+67233855 None:intergenic
CACAACAAAGTTCCTTTCGA+AGG 0.499101 3_2:-67234058 Msa0419310:CDS
AAGGACGAAATCAAGCCTAA+TGG 0.501353 3_2:-67233641 Msa0419310:CDS
CTGTAATCCTTACTTCATGC+TGG 0.522064 3_2:-67233862 Msa0419310:CDS
GTACTGTCACACTGAGGCAA+GGG 0.522922 3_2:-67234147 Msa0419310:CDS
CTTGAAGGAATTGCAGGTGT+AGG 0.530115 3_2:+67233761 None:intergenic
TATCTGTGTGAAGTTGGGTC+GGG 0.549535 3_2:+67233823 None:intergenic
TGCAGGTGTAGGAGGCGAAA+CGG 0.551922 3_2:+67233772 None:intergenic
GAAGGAATTGCAGGTGTAGG+AGG 0.556650 3_2:+67233764 None:intergenic
CATAGAATCTTGTCTAAAGA+GGG 0.557072 3_2:-67233538 Msa0419310:three_prime_UTR
CGGCGATACACCTAGAGTAA+AGG 0.566162 3_2:-67233660 Msa0419310:CDS
TTATCTGTGTGAAGTTGGGT+CGG 0.573170 3_2:+67233822 None:intergenic
AGGTTTGACATGCCTTCGAA+AGG 0.577484 3_2:+67234046 None:intergenic
ATGGCAAAACGTGATGACAC+CGG 0.589230 3_2:-67233680 Msa0419310:CDS
CCATTAAGGAGCTCGTACCA+GGG 0.592520 3_2:+67233699 None:intergenic
AGTACTGTCACACTGAGGCA+AGG 0.598182 3_2:-67234148 Msa0419310:CDS
CCTTACTTCATGCTGGCAAG+AGG 0.616813 3_2:-67233855 Msa0419310:CDS
AACACTGGCTGAAACAGCAA+AGG 0.617534 3_2:+67233615 None:intergenic
CTTTACTCTAGGTGTATCGC+CGG 0.619229 3_2:+67233661 None:intergenic
TACTGTCACACTGAGGCAAG+GGG 0.631225 3_2:-67234146 Msa0419310:CDS
CATTAAGGAGCTCGTACCAG+GGG 0.631355 3_2:+67233700 None:intergenic
TCGGGAAGATGATCCGCACT+CGG 0.635028 3_2:+67233893 None:intergenic
TTAGAATGTAAGGCCGAGTG+CGG 0.636499 3_2:-67233906 Msa0419310:intron
GTGTTGCCACCAGAATCCCC+TGG 0.645611 3_2:-67233716 Msa0419310:CDS
TGTTCAAGAGTTAATAACAC+TGG 0.654358 3_2:+67233600 None:intergenic
CTCGTACCAGGGGATTCTGG+TGG 0.665696 3_2:+67233710 None:intergenic
AGGGGATTCTGGTGGCAACA+CGG 0.676571 3_2:+67233718 None:intergenic
TATACAGTACTGTCACACTG+AGG 0.785024 3_2:-67234153 Msa0419310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTGGTTTTTAGAATGTA+AGG - chr3_2:67233717-67233736 Msa0419310:CDS 25.0%
!!! AAAGATAATTTAGGAGCATT+TGG + chr3_2:67233838-67233857 None:intergenic 25.0%
! AAACGGTGTAAAGATAATTT+AGG + chr3_2:67233847-67233866 None:intergenic 25.0%
! ATTTACCAAGTCATCAAAAA+AGG + chr3_2:67234123-67234142 None:intergenic 25.0%
!! ATAGCTTTGCAATCGTATTT+TGG - chr3_2:67233543-67233562 Msa0419310:three_prime_UTR 30.0%
!! TAGCTTTGCAATCGTATTTT+GGG - chr3_2:67233544-67233563 Msa0419310:three_prime_UTR 30.0%
ATGATCTTAGATTACCTTGA+AGG + chr3_2:67233633-67233652 None:intergenic 30.0%
AATATTCTTGAAGGAATTGC+AGG + chr3_2:67233881-67233900 None:intergenic 30.0%
TCAGAAGTGAATATTCTTGA+AGG + chr3_2:67233890-67233909 None:intergenic 30.0%
TGTTCAAGAGTTAATAACAC+TGG + chr3_2:67234036-67234055 None:intergenic 30.0%
! AGTGTTATTAACTCTTGAAC+AGG - chr3_2:67234035-67234054 Msa0419310:CDS 30.0%
!! CAGGTCTTTAAAGTACAAAA+TGG - chr3_2:67234054-67234073 Msa0419310:CDS 30.0%
ACATAGAATCTTGTCTAAAG+AGG - chr3_2:67234094-67234113 Msa0419310:CDS 30.0%
CATAGAATCTTGTCTAAAGA+GGG - chr3_2:67234095-67234114 Msa0419310:CDS 30.0%
!! AGCTATAAGCATCGACTTTA+TGG + chr3_2:67233529-67233548 None:intergenic 35.0%
AAGGATTACAGCCAATTCTT+CGG + chr3_2:67233762-67233781 None:intergenic 35.0%
TGGATTATCTGTGTGAAGTT+GGG + chr3_2:67233818-67233837 None:intergenic 35.0%
TTGGATTATCTGTGTGAAGT+TGG + chr3_2:67233819-67233838 None:intergenic 35.0%
TTGATTTCGTCCTTTACTCT+AGG + chr3_2:67233986-67234005 None:intergenic 35.0%
AAACAGCAAAGGAAACCATT+AGG + chr3_2:67234010-67234029 None:intergenic 35.0%
TATACAGTACTGTCACACTG+AGG - chr3_2:67233480-67233499 Msa0419310:three_prime_UTR 40.0%
CACAACAAAGTTCCTTTCGA+AGG - chr3_2:67233575-67233594 Msa0419310:three_prime_UTR 40.0%
AGGATTACAGCCAATTCTTC+GGG + chr3_2:67233761-67233780 None:intergenic 40.0%
CTGTAATCCTTACTTCATGC+TGG - chr3_2:67233771-67233790 Msa0419310:CDS 40.0%
TTATCTGTGTGAAGTTGGGT+CGG + chr3_2:67233814-67233833 None:intergenic 40.0%
! GTCATCACGTTTTGCCATTA+AGG + chr3_2:67233951-67233970 None:intergenic 40.0%
AAGGACGAAATCAAGCCTAA+TGG - chr3_2:67233992-67234011 Msa0419310:intron 40.0%
!!! GGGATCCTTTTTTGATGACT+TGG - chr3_2:67234115-67234134 Msa0419310:CDS 40.0%
AGGTTTGACATGCCTTCGAA+AGG + chr3_2:67233590-67233609 None:intergenic 45.0%
! CGCGTGTCAACAGTTTTTGA+CGG + chr3_2:67233696-67233715 None:intergenic 45.0%
! AACTGTTGACACGCGTCTTT+TGG - chr3_2:67233701-67233720 Msa0419310:CDS 45.0%
TTAGAATGTAAGGCCGAGTG+CGG - chr3_2:67233727-67233746 Msa0419310:CDS 45.0%
GGATCATCTTCCCGAAGAAT+TGG - chr3_2:67233748-67233767 Msa0419310:CDS 45.0%
TATCTGTGTGAAGTTGGGTC+GGG + chr3_2:67233813-67233832 None:intergenic 45.0%
CTTGAAGGAATTGCAGGTGT+AGG + chr3_2:67233875-67233894 None:intergenic 45.0%
ATGGCAAAACGTGATGACAC+CGG - chr3_2:67233953-67233972 Msa0419310:intron 45.0%
CTTTACTCTAGGTGTATCGC+CGG + chr3_2:67233975-67233994 None:intergenic 45.0%
! AACACTGGCTGAAACAGCAA+AGG + chr3_2:67234021-67234040 None:intergenic 45.0%
AGTACTGTCACACTGAGGCA+AGG - chr3_2:67233485-67233504 Msa0419310:three_prime_UTR 50.0%
GTACTGTCACACTGAGGCAA+GGG - chr3_2:67233486-67233505 Msa0419310:three_prime_UTR 50.0%
TACTGTCACACTGAGGCAAG+GGG - chr3_2:67233487-67233506 Msa0419310:three_prime_UTR 50.0%
CTGCAGCATATGCAGCTTGA+AGG + chr3_2:67233610-67233629 None:intergenic 50.0%
TGCATATGCTGCAGCCTTCA+AGG - chr3_2:67233616-67233635 Msa0419310:CDS 50.0%
CCTCTTGCCAGCATGAAGTA+AGG + chr3_2:67233781-67233800 None:intergenic 50.0%
CCTTACTTCATGCTGGCAAG+AGG - chr3_2:67233778-67233797 Msa0419310:CDS 50.0%
GAAGGAATTGCAGGTGTAGG+AGG + chr3_2:67233872-67233891 None:intergenic 50.0%
CATTAAGGAGCTCGTACCAG+GGG + chr3_2:67233936-67233955 None:intergenic 50.0%
CCATTAAGGAGCTCGTACCA+GGG + chr3_2:67233937-67233956 None:intergenic 50.0%
CGGCGATACACCTAGAGTAA+AGG - chr3_2:67233973-67233992 Msa0419310:intron 50.0%
TCGGGAAGATGATCCGCACT+CGG + chr3_2:67233743-67233762 None:intergenic 55.0%
TGCAGGTGTAGGAGGCGAAA+CGG + chr3_2:67233864-67233883 None:intergenic 55.0%
AGGGGATTCTGGTGGCAACA+CGG + chr3_2:67233918-67233937 None:intergenic 55.0%
CCCTGGTACGAGCTCCTTAA+TGG - chr3_2:67233934-67233953 Msa0419310:intron 55.0%
GCCATTAAGGAGCTCGTACC+AGG + chr3_2:67233938-67233957 None:intergenic 55.0%
GTGTTGCCACCAGAATCCCC+TGG - chr3_2:67233917-67233936 Msa0419310:intron 60.0%
CTCGTACCAGGGGATTCTGG+TGG + chr3_2:67233926-67233945 None:intergenic 60.0%
GAGCTCGTACCAGGGGATTC+TGG + chr3_2:67233929-67233948 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 67233479 67234176 67233479 ID=Msa0419310;Name=Msa0419310
chr3_2 mRNA 67233479 67234176 67233479 ID=Msa0419310-mRNA-1;Parent=Msa0419310;Name=Msa0419310-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|1|0.5|1|1|1|2|131|157
chr3_2 exon 67233479 67233924 67233479 ID=Msa0419310-mRNA-1:exon:15622;Parent=Msa0419310-mRNA-1
chr3_2 exon 67234018 67234176 67234018 ID=Msa0419310-mRNA-1:exon:15621;Parent=Msa0419310-mRNA-1
chr3_2 CDS 67234018 67234176 67234018 ID=Msa0419310-mRNA-1:cds;Parent=Msa0419310-mRNA-1
chr3_2 CDS 67233610 67233924 67233610 ID=Msa0419310-mRNA-1:cds;Parent=Msa0419310-mRNA-1
chr3_2 three_prime_UTR 67233479 67233609 67233479 ID=Msa0419310-mRNA-1:three_prime_utr;Parent=Msa0419310-mRNA-1
Gene Sequence

>Msa0419310

TTATACAGTACTGTCACACTGAGGCAAGGGGAGAAGAAGCATTATAACCATAAAGTCGATGCTTATAGCTTTGCAATCGTATTTTGGGAGCTCTTGCACAACAAAGTTCCTTTCGAAGGCATGTCAAACCTTCAAGCTGCATATGCTGCAGCCTTCAAGAATGTAAGGCCGAGTGCGGATCATCTTCCCGAAGAATTGGCTGTAATCCTTACTTCATGCTGGCAAGAGGATGCAAATGCCCGACCCAACTTCACACAGATAATCCAAATGCTCCTAAATTATCTTTACACCGTTTCGCCTCCTACACCTGCAATTCCTTCAAGAATATTCACTTCTGAGAACACCGTGTTGCCACCAGAATCCCCTGGTACGAGCTCCTTAATGGCAAAACGTGATGACACCGGCGATACACCTAGAGTAAAGGACGAAATCAAGCCTAATGGTTTCCTTTGCTGTTTCAGCCAGTGTTATTAA

Protein sequence

>Msa0419310

LYSTVTLRQGEKKHYNHKVDAYSFAIVFWELLHNKVPFEGMSNLQAAYAAAFKNVRPSADHLPEELAVILTSCWQEDANARPNFTQIIQMLLNYLYTVSPPTPAIPSRIFTSENTVLPPESPGTSSLMAKRDDTGDTPRVKDEIKPNGFLCCFSQCY*