AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0623280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623280 MtrunA17_Chr1g0212841 93.868 212 13 0 1 212 1 212 1.60e-146 406
Msa0623280 MtrunA17_Chr3g0111011 60.096 208 83 0 3 210 55 262 1.81e-93 274
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0623280 AT1G75270.1 73.709 213 55 1 1 212 1 213 5.83e-115 327
Msa0623280 AT1G19570.1 74.057 212 54 1 1 211 1 212 6.29e-115 327
Msa0623280 AT5G16710.1 60.577 208 82 0 3 210 49 256 3.46e-96 281
Msa0623280 AT1G19550.1 55.172 174 51 3 41 211 3 152 3.48e-60 186

Find 87 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTCAAGTTTGATGGTAAA+TGG 0.194555 4_3:+464760 Msa0623280:CDS
TGAGGAAAGTTTCTTGAAAA+AGG 0.205482 4_3:-467881 None:intergenic
TTGGACTATTCCTGAGAATT+TGG 0.207787 4_3:+465475 Msa0623280:CDS
AAGAGAACCCTTTGGGAAAA+TGG 0.223837 4_3:-464539 None:intergenic
ATTTCAGGGGATCAAATATA+TGG 0.237821 4_3:+465154 Msa0623280:intron
CACAGCTGTTGATTTGAGTT+TGG 0.323954 4_3:+465406 Msa0623280:CDS
CTACTTCATGCATTGACCTT+TGG 0.348598 4_3:-467166 None:intergenic
CCTGATTCTGATGTTATTGT+TGG 0.361346 4_3:+464786 Msa0623280:CDS
TGCTTTGGATGAACATCTTA+AGG 0.363226 4_3:+465251 Msa0623280:CDS
ACCACTAGATGGTACAGTTT+TGG 0.363899 4_3:-465431 None:intergenic
CAAAGTTAAGAGAACCCTTT+GGG 0.367512 4_3:-464546 None:intergenic
CATTCACCCAGGTTCGAATC+TGG 0.374806 4_3:+467737 Msa0623280:three_prime_UTR
TAACAGGGTCCATTTGTTGC+TGG 0.379644 4_3:+465374 Msa0623280:intron
GTTAGCAGAACTCAATGCTT+TGG 0.381868 4_3:+465236 Msa0623280:CDS
GTTCCAGTGGTCAAGTTTGA+TGG 0.383379 4_3:+464753 Msa0623280:CDS
CCAAAGGGTTCTCTTAACTT+TGG 0.391179 4_3:+464547 Msa0623280:CDS
TGGACCTTTGCCAAATTCTC+AGG 0.406140 4_3:-465485 None:intergenic
TTTAGACTACTGAGTTGTTT+CGG 0.408993 4_3:+467535 Msa0623280:three_prime_UTR
TTGACAATGCTGATGTTGTT+AGG 0.412327 4_3:+467708 Msa0623280:three_prime_UTR
AACAGGGTCCATTTGTTGCT+GGG 0.421851 4_3:+465375 Msa0623280:intron
GACATACTCCTCTGCAGCCT+TGG 0.421971 4_3:-467128 None:intergenic
GCAGAGGAGTATGTCATTGC+TGG 0.422618 4_3:+467136 Msa0623280:CDS
GGAGAAACAAGAGATGGTTC+AGG 0.422879 4_3:-464828 None:intergenic
CCAAAGTTAAGAGAACCCTT+TGG 0.428562 4_3:-464547 None:intergenic
TATTATTAAGAAATAAGAAT+AGG 0.428644 4_3:+467237 Msa0623280:three_prime_UTR
TTAATCAAATGGATGTTGTA+TGG 0.432983 4_3:-464584 None:intergenic
AGAGTACCATTGGGGTTTGT+CGG 0.436593 4_3:-464612 None:intergenic
CTCATTCTCATCATCGCAAA+TGG 0.445448 4_3:+464369 Msa0623280:exon
TGTCGGAGATATTAATCAAA+TGG 0.447813 4_3:-464595 None:intergenic
GTCAATTGAGCTAAAGCTTA+CGG 0.450367 4_3:-467690 None:intergenic
ACCTTCTCCCCAGCAACAAA+TGG 0.454458 4_3:-465383 None:intergenic
GTTGGTGCTCCAACTGTTCT+CGG 0.458021 4_3:+464418 Msa0623280:CDS
GTCTAAAGTTTGTCGCGATT+AGG 0.459085 4_3:-467519 None:intergenic
GGTCAAGTTTGATGGTAAAT+GGG 0.460396 4_3:+464761 Msa0623280:CDS
AGCTAAAGCTTACGGACTTA+CGG 0.462612 4_3:-467682 None:intergenic
TGAATCCTTGCTCTTCAGAA+AGG 0.466931 4_3:-465190 None:intergenic
CCAACAATAACATCAGAATC+AGG 0.477453 4_3:-464786 None:intergenic
GGCTCTTGAGGTTGCTGTCA+AGG 0.478726 4_3:+464390 Msa0623280:CDS
ACCACTGGAACCTTCCCTCC+AGG 0.482366 4_3:-464741 None:intergenic
AAGAGCAAGGATTCAAATGA+TGG 0.484327 4_3:+465198 Msa0623280:CDS
TCCATTTGTTGCTGGGGAGA+AGG 0.485126 4_3:+465382 Msa0623280:CDS
GACCAAGTGCTACCACTAGA+TGG 0.490749 4_3:-465442 None:intergenic
CGTGACCTTTCTGAAGAGCA+AGG 0.493675 4_3:+465185 Msa0623280:CDS
TTACCATCAAACTTGACCAC+TGG 0.501479 4_3:-464756 None:intergenic
CACGATTCCAGATTCGAACC+TGG 0.503446 4_3:-467744 None:intergenic
TCTCCTTTGATGCTATTCAA+TGG 0.506421 4_3:+467558 Msa0623280:three_prime_UTR
CAGATTCGAACCTGGGTGAA+TGG 0.506777 4_3:-467736 None:intergenic
GTTGCTGTCAAGGCTGCTGT+TGG 0.509413 4_3:+464400 Msa0623280:CDS
GAATGAGAAGAGAACAAAGA+AGG 0.509847 4_3:-464353 None:intergenic
CTTCTCTATAATAAATAAGT+AGG 0.515000 4_3:+467308 Msa0623280:three_prime_UTR
CACTTGGTCACTTCAAGAGT+TGG 0.517047 4_3:+465456 Msa0623280:CDS
TTTCAGGGGATCAAATATAT+GGG 0.518607 4_3:+465155 Msa0623280:intron
AAACACAAGGGTGTCATGGC+AGG 0.528390 4_3:+467441 Msa0623280:three_prime_UTR
TGGACATTTCAGCCAGCCAA+TGG 0.544744 4_3:-467374 None:intergenic
TACACTGAGGAAAATTGAGC+AGG 0.548119 4_3:-464849 None:intergenic
AGGAGTATGTCATTGCTGGA+TGG 0.548576 4_3:+467140 Msa0623280:CDS
CATTGCTGGATGGGCACCAA+AGG 0.555766 4_3:+467150 Msa0623280:CDS
TGTGGGAACTAGGCAAAGGT+TGG 0.557046 4_3:+467834 Msa0623280:three_prime_UTR
ATCATCGCAAATGGCTCTTG+AGG 0.559050 4_3:+464378 Msa0623280:exon
GGAAGTGAATCCTGGAGGGA+AGG 0.560932 4_3:+464731 Msa0623280:CDS
GGGTTGTAGTTAAATTCATG+TGG 0.566505 4_3:+467655 Msa0623280:three_prime_UTR
GAGAAACAAGAGATGGTTCA+GGG 0.568330 4_3:-464827 None:intergenic
ATTAAGAAATAAGAATAGGA+TGG 0.569904 4_3:+467241 Msa0623280:three_prime_UTR
CCTAAAACACAAGGGTGTCA+TGG 0.572642 4_3:+467437 Msa0623280:three_prime_UTR
GGAGTATGTCATTGCTGGAT+GGG 0.575558 4_3:+467141 Msa0623280:CDS
CGAGAAAACCAAGGCTGCAG+AGG 0.575652 4_3:+467120 Msa0623280:CDS
TGACATGGTTCTAACTTCTG+AGG 0.581715 4_3:-467899 None:intergenic
TTACCATTGAATAGCATCAA+AGG 0.591671 4_3:-467561 None:intergenic
ACAGGGTCCATTTGTTGCTG+GGG 0.596245 4_3:+465376 Msa0623280:intron
GGTTGTAGTTAAATTCATGT+GGG 0.596971 4_3:+467656 Msa0623280:three_prime_UTR
TACCATCTAGTGGTAGCACT+TGG 0.606458 4_3:+465440 Msa0623280:CDS
TCCTGGAGGGAAGGTTCCAG+TGG 0.613379 4_3:+464740 Msa0623280:CDS
ACGATTCCAGATTCGAACCT+GGG 0.620046 4_3:-467743 None:intergenic
ATCATGTGGGAACTAGGCAA+AGG 0.620931 4_3:+467830 Msa0623280:three_prime_UTR
TATTCCTGAGAATTTGGCAA+AGG 0.623001 4_3:+465481 Msa0623280:CDS
GTACAGTCTCCGAGAACAGT+TGG 0.629766 4_3:-464427 None:intergenic
TTTGTACCTTGATGTAGTTG+TGG 0.632536 4_3:-465505 None:intergenic
AAAGGTCCACAACTACATCA+AGG 0.634770 4_3:+465499 Msa0623280:CDS
TGAGCAGGAGAAACAAGAGA+TGG 0.641215 4_3:-464834 None:intergenic
TTAGGTTGCACCATTCACCC+AGG 0.645366 4_3:+467726 Msa0623280:three_prime_UTR
TAAATTCGTCCTAAAACACA+AGG 0.648041 4_3:+467428 Msa0623280:three_prime_UTR
TTGAATTTAGAGTACCATTG+GGG 0.655225 4_3:-464620 None:intergenic
ACCAAAACTGTACCATCTAG+TGG 0.659489 4_3:+465430 Msa0623280:CDS
GCTCGATCACAGTGCATGTG+TGG 0.663154 4_3:-467212 None:intergenic
ATATCTCCGACAAACCCCAA+TGG 0.672245 4_3:+464606 Msa0623280:CDS
TGAATCATTCGAGAAAACCA+AGG 0.685046 4_3:+467111 Msa0623280:CDS
AAATTCGTCCTAAAACACAA+GGG 0.747294 4_3:+467429 Msa0623280:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTTTTAGAGAAAA+GGG - chr4_3:466313-466332 None:intergenic 10.0%
!!! AATCAAATTTTGAAATTTAA+AGG - chr4_3:466728-466747 None:intergenic 10.0%
!! TTGATAAATAATATATTACA+CGG - chr4_3:466861-466880 None:intergenic 10.0%
!! TATTATTAAGAAATAAGAAT+AGG + chr4_3:467237-467256 Msa0623280:three_prime_UTR 10.0%
!!! TATTTTTTTTTATAAACAAG+TGG - chr4_3:467807-467826 None:intergenic 10.0%
!! AAAAAAAAATAAAAATCATG+TGG + chr4_3:467816-467835 Msa0623280:three_prime_UTR 10.0%
!! AAAAAAAATAAAAATCATGT+GGG + chr4_3:467817-467836 Msa0623280:three_prime_UTR 10.0%
!!! TTTTATTTCTTTGAATTTTG+TGG + chr4_3:464675-464694 Msa0623280:intron 15.0%
!!! TTTATTTATTTGTAGGTTTT+TGG + chr4_3:464710-464729 Msa0623280:intron 15.0%
!!! TTTGAAAGTATAAAAATAGA+GGG - chr4_3:464893-464912 None:intergenic 15.0%
!! ATAAATTCACATATATACAT+TGG - chr4_3:465277-465296 None:intergenic 15.0%
!!! TTTTTTTTTTTAGAGAAAAG+GGG - chr4_3:466312-466331 None:intergenic 15.0%
!!! GATTTTTTTTTTTAGAGAAA+AGG - chr4_3:466314-466333 None:intergenic 15.0%
!!! AAATTTTGAAATTTAAAGGA+GGG - chr4_3:466724-466743 None:intergenic 15.0%
!!! TTAATAGGTTATATAACTTT+TGG + chr4_3:466800-466819 Msa0623280:intron 15.0%
!!! AAGATTTTAAGTTCTTATTT+TGG + chr4_3:467622-467641 Msa0623280:three_prime_UTR 15.0%
!!! AGATTTTAAGTTCTTATTTT+GGG + chr4_3:467623-467642 Msa0623280:three_prime_UTR 15.0%
!!! TTTCATTTGTTTTCATTTTC+AGG + chr4_3:464515-464534 Msa0623280:intron 20.0%
!!! GTTTGAAAGTATAAAAATAG+AGG - chr4_3:464894-464913 None:intergenic 20.0%
!!! ATGTTACTTTCTATATTTTG+TGG + chr4_3:464959-464978 Msa0623280:intron 20.0%
!!! TGTTACTTTCTATATTTTGT+GGG + chr4_3:464960-464979 Msa0623280:intron 20.0%
!! ACATTTCCAATATCAAATTA+TGG + chr4_3:465914-465933 Msa0623280:intron 20.0%
!! CATTTCCAATATCAAATTAT+GGG + chr4_3:465915-465934 Msa0623280:intron 20.0%
!! TATGCTAAAAAAATGTTACA+TGG - chr4_3:466208-466227 None:intergenic 20.0%
!!! TTTCATTTTTTTGGATAAAG+TGG - chr4_3:466336-466355 None:intergenic 20.0%
!!! AATTTTGAAATTTAAAGGAG+GGG - chr4_3:466723-466742 None:intergenic 20.0%
!!! CAAATTTTGAAATTTAAAGG+AGG - chr4_3:466725-466744 None:intergenic 20.0%
!! TCATCACATTATTAGTTAAT+AGG + chr4_3:466785-466804 Msa0623280:intron 20.0%
!! TATATGAATTGATTGATTGA+TGG + chr4_3:467054-467073 Msa0623280:intron 20.0%
!! ATTAAGAAATAAGAATAGGA+TGG + chr4_3:467241-467260 Msa0623280:three_prime_UTR 20.0%
!! CTTCTCTATAATAAATAAGT+AGG + chr4_3:467308-467327 Msa0623280:three_prime_UTR 20.0%
!!! TTCACATTTTAAAGCTTATA+TGG - chr4_3:467397-467416 None:intergenic 20.0%
! TTAATCAAATGGATGTTGTA+TGG - chr4_3:464587-464606 None:intergenic 25.0%
! GTTAATTAGCTATCAATTAC+AGG - chr4_3:465332-465351 None:intergenic 25.0%
!!! TACAAAGTTGTTTTCTGTAA+TGG + chr4_3:465522-465541 Msa0623280:intron 25.0%
! ATGGATGTAATCTTATCTTA+TGG + chr4_3:465541-465560 Msa0623280:intron 25.0%
! AAGTACAACAAAAAAACAGT+TGG - chr4_3:465681-465700 None:intergenic 25.0%
!!! GGGTTTTAAATCTTACATAT+TGG + chr4_3:466091-466110 Msa0623280:intron 25.0%
!! ATTGGATAGAGATTTCTTTT+AGG + chr4_3:466109-466128 Msa0623280:intron 25.0%
! CAAGTGATTAAATTTATCTC+TGG + chr4_3:466230-466249 Msa0623280:intron 25.0%
!!! AAGCATGCTTTTCATTTTTT+TGG - chr4_3:466345-466364 None:intergenic 25.0%
! ATTGTAGAAGTTGACAATTA+GGG + chr4_3:466494-466513 Msa0623280:intron 25.0%
! TCATAAATCAAAGAAACAAC+AGG - chr4_3:466588-466607 None:intergenic 25.0%
! AAATACATCAATACTCCTAA+AGG - chr4_3:466914-466933 None:intergenic 25.0%
!! GTTGTAGGTATTGTAAATTA+AGG - chr4_3:466973-466992 None:intergenic 25.0%
!!! TTTTTTTCAGCAAATGCAAA+AGG - chr4_3:467001-467020 None:intergenic 25.0%
!!! ACTGTGTTTTATTAATCCAT+TGG + chr4_3:467358-467377 Msa0623280:three_prime_UTR 25.0%
!! CTGAGATTTATTTCTGTTAC+CGG + chr4_3:464474-464493 Msa0623280:intron 30.0%
AATCGTGAAAAATGTAGAAC+CGG - chr4_3:464496-464515 None:intergenic 30.0%
TGTCGGAGATATTAATCAAA+TGG - chr4_3:464598-464617 None:intergenic 30.0%
! TTGAATTTAGAGTACCATTG+GGG - chr4_3:464623-464642 None:intergenic 30.0%
! GTTGAATTTAGAGTACCATT+GGG - chr4_3:464624-464643 None:intergenic 30.0%
! AGTTGAATTTAGAGTACCAT+TGG - chr4_3:464625-464644 None:intergenic 30.0%
TTGAATACCATCTTGCAAAT+GGG - chr4_3:464929-464948 None:intergenic 30.0%
! TTTCTATATTTTGTGGGATG+AGG + chr4_3:464966-464985 Msa0623280:intron 30.0%
!!! GTATACATGCTTGTTTTGAT+TGG + chr4_3:465028-465047 Msa0623280:intron 30.0%
ATTTCAGGGGATCAAATATA+TGG + chr4_3:465154-465173 Msa0623280:intron 30.0%
TTTCAGGGGATCAAATATAT+GGG + chr4_3:465155-465174 Msa0623280:intron 30.0%
TTATGAAGAAACTTCAAGTG+TGG - chr4_3:465710-465729 None:intergenic 30.0%
!!! TCTTTGTTTTAAAGCAAAGC+TGG + chr4_3:465842-465861 Msa0623280:intron 30.0%
GCAAACCCATAATTTGATAT+TGG - chr4_3:465923-465942 None:intergenic 30.0%
GATATGGATGAAAGATGTAT+GGG - chr4_3:465995-466014 None:intergenic 30.0%
TGATATGGATGAAAGATGTA+TGG - chr4_3:465996-466015 None:intergenic 30.0%
!! GTACTTCAATCGTTTTGATA+TGG - chr4_3:466011-466030 None:intergenic 30.0%
TTAAAGTCTGAACTTAAAGC+TGG + chr4_3:466070-466089 Msa0623280:intron 30.0%
TAAAGTCTGAACTTAAAGCT+GGG + chr4_3:466071-466090 Msa0623280:intron 30.0%
!! AATTATGCGTGGCTATTTTA+GGG + chr4_3:466136-466155 Msa0623280:intron 30.0%
!!! TTTTTTTTAGAGAAAAGGGG+AGG - chr4_3:466309-466328 None:intergenic 30.0%
AATTGTCAACTTCTACAATC+TGG - chr4_3:466493-466512 None:intergenic 30.0%
GATTGTAGAAGTTGACAATT+AGG + chr4_3:466493-466512 Msa0623280:intron 30.0%
TAGTTCACATTAACTAGTCA+TGG + chr4_3:466540-466559 Msa0623280:intron 30.0%
ATCTTCAGACTTAAAGTTGA+TGG - chr4_3:466667-466686 None:intergenic 30.0%
TATATTACACGGATTCAATG+TGG - chr4_3:466850-466869 None:intergenic 30.0%
ATATATCGAGTTAGACCTTT+AGG + chr4_3:466896-466915 Msa0623280:intron 30.0%
TACCTAAGAGATTCATTGTA+TGG - chr4_3:466942-466961 None:intergenic 30.0%
! GGTGTAACTTATCATTTTGA+AGG + chr4_3:467262-467281 Msa0623280:three_prime_UTR 30.0%
!! TTCATGTGTGTTTTGTTGAT+GGG + chr4_3:467334-467353 Msa0623280:three_prime_UTR 30.0%
!! TGTTTTATTAATCCATTGGC+TGG + chr4_3:467362-467381 Msa0623280:three_prime_UTR 30.0%
TAAATTCGTCCTAAAACACA+AGG + chr4_3:467428-467447 Msa0623280:three_prime_UTR 30.0%
AAATTCGTCCTAAAACACAA+GGG + chr4_3:467429-467448 Msa0623280:three_prime_UTR 30.0%
TTTAGACTACTGAGTTGTTT+CGG + chr4_3:467535-467554 Msa0623280:three_prime_UTR 30.0%
TTACCATTGAATAGCATCAA+AGG - chr4_3:467564-467583 None:intergenic 30.0%
GGTTGTAGTTAAATTCATGT+GGG + chr4_3:467656-467675 Msa0623280:three_prime_UTR 30.0%
ATAAAAATCATGTGGGAACT+AGG + chr4_3:467824-467843 Msa0623280:three_prime_UTR 30.0%
GAAAGTTTCTTGAAAAAGGA+GGG - chr4_3:467880-467899 None:intergenic 30.0%
TGAGGAAAGTTTCTTGAAAA+AGG - chr4_3:467884-467903 None:intergenic 30.0%
GAATGAGAAGAGAACAAAGA+AGG - chr4_3:464356-464375 None:intergenic 35.0%
CAAAGTTAAGAGAACCCTTT+GGG - chr4_3:464549-464568 None:intergenic 35.0%
! TGGTCAAGTTTGATGGTAAA+TGG + chr4_3:464760-464779 Msa0623280:CDS 35.0%
! GGTCAAGTTTGATGGTAAAT+GGG + chr4_3:464761-464780 Msa0623280:CDS 35.0%
CCAACAATAACATCAGAATC+AGG - chr4_3:464789-464808 None:intergenic 35.0%
CCTGATTCTGATGTTATTGT+TGG + chr4_3:464786-464805 Msa0623280:CDS 35.0%
TGAATACCATCTTGCAAATG+GGG - chr4_3:464928-464947 None:intergenic 35.0%
CTTGAATACCATCTTGCAAA+TGG - chr4_3:464930-464949 None:intergenic 35.0%
TTCACGATGTTCACAATTTC+AGG + chr4_3:465139-465158 Msa0623280:intron 35.0%
TCACGATGTTCACAATTTCA+GGG + chr4_3:465140-465159 Msa0623280:intron 35.0%
AAGAGCAAGGATTCAAATGA+TGG + chr4_3:465198-465217 Msa0623280:CDS 35.0%
! TGCTTTGGATGAACATCTTA+AGG + chr4_3:465251-465270 Msa0623280:CDS 35.0%
AATTAACCACACTAACATGC+TGG + chr4_3:465345-465364 Msa0623280:intron 35.0%
TTGGACTATTCCTGAGAATT+TGG + chr4_3:465475-465494 Msa0623280:CDS 35.0%
TATTCCTGAGAATTTGGCAA+AGG + chr4_3:465481-465500 Msa0623280:CDS 35.0%
TTTGTACCTTGATGTAGTTG+TGG - chr4_3:465508-465527 None:intergenic 35.0%
CGCAAAAGACATGACTAAAA+GGG - chr4_3:465569-465588 None:intergenic 35.0%
ACGCAAAAGACATGACTAAA+AGG - chr4_3:465570-465589 None:intergenic 35.0%
TTCACAGCTGTTCAATTCAA+TGG - chr4_3:465595-465614 None:intergenic 35.0%
TGTAGTCAACATAAAGTGTG+TGG + chr4_3:465771-465790 Msa0623280:intron 35.0%
! CAATTTGCTTCTGGAAAGAT+TGG - chr4_3:465882-465901 None:intergenic 35.0%
! TGTGAACATCAATTTGCTTC+TGG - chr4_3:465891-465910 None:intergenic 35.0%
!! TGCTGGTGTCATGAAAAAAT+TGG + chr4_3:465949-465968 Msa0623280:intron 35.0%
!! TGGTGTCATGAAAAAATTGG+TGG + chr4_3:465952-465971 Msa0623280:intron 35.0%
!! TTTTAGGTCTCAATTATGCG+TGG + chr4_3:466125-466144 Msa0623280:intron 35.0%
!! CAATTATGCGTGGCTATTTT+AGG + chr4_3:466135-466154 Msa0623280:intron 35.0%
GGTGAGTTAAGAAATAGAGA+AGG - chr4_3:466288-466307 None:intergenic 35.0%
!! CATGCTTTTCTTATTTGCCA+GGG + chr4_3:466358-466377 Msa0623280:intron 35.0%
! GTTCAATGAAGCATCAGTAA+GGG - chr4_3:466436-466455 None:intergenic 35.0%
! AGTTCAATGAAGCATCAGTA+AGG - chr4_3:466437-466456 None:intergenic 35.0%
GTCCATACAATGAATCTCTT+AGG + chr4_3:466937-466956 Msa0623280:intron 35.0%
! ATGCAAAAGGCTTTAGTTGT+AGG - chr4_3:466988-467007 None:intergenic 35.0%
TGAATCATTCGAGAAAACCA+AGG + chr4_3:467111-467130 Msa0623280:CDS 35.0%
!! GTTCATGTGTGTTTTGTTGA+TGG + chr4_3:467333-467352 Msa0623280:three_prime_UTR 35.0%
TCTCCTTTGATGCTATTCAA+TGG + chr4_3:467558-467577 Msa0623280:three_prime_UTR 35.0%
!!! TCTTATTTTGGGACAGCAAT+AGG + chr4_3:467634-467653 Msa0623280:three_prime_UTR 35.0%
!!! CTTATTTTGGGACAGCAATA+GGG + chr4_3:467635-467654 Msa0623280:three_prime_UTR 35.0%
GGGTTGTAGTTAAATTCATG+TGG + chr4_3:467655-467674 Msa0623280:three_prime_UTR 35.0%
GTCAATTGAGCTAAAGCTTA+CGG - chr4_3:467693-467712 None:intergenic 35.0%
TTGACAATGCTGATGTTGTT+AGG + chr4_3:467708-467727 Msa0623280:three_prime_UTR 35.0%
GGAAAGTTTCTTGAAAAAGG+AGG - chr4_3:467881-467900 None:intergenic 35.0%
CTCATTCTCATCATCGCAAA+TGG + chr4_3:464369-464388 Msa0623280:exon 40.0%
!! TTTCAGGTCCATTTTCCCAA+AGG + chr4_3:464531-464550 Msa0623280:intron 40.0%
! TTCAGGTCCATTTTCCCAAA+GGG + chr4_3:464532-464551 Msa0623280:intron 40.0%
! AAGAGAACCCTTTGGGAAAA+TGG - chr4_3:464542-464561 None:intergenic 40.0%
CCAAAGTTAAGAGAACCCTT+TGG - chr4_3:464550-464569 None:intergenic 40.0%
CCAAAGGGTTCTCTTAACTT+TGG + chr4_3:464547-464566 Msa0623280:CDS 40.0%
!! AGGTTTTTGGAAGTGAATCC+TGG + chr4_3:464723-464742 Msa0623280:intron 40.0%
!! TTTTTGGAAGTGAATCCTGG+AGG + chr4_3:464726-464745 Msa0623280:CDS 40.0%
!! TTTTGGAAGTGAATCCTGGA+GGG + chr4_3:464727-464746 Msa0623280:CDS 40.0%
TTACCATCAAACTTGACCAC+TGG - chr4_3:464759-464778 None:intergenic 40.0%
GAGAAACAAGAGATGGTTCA+GGG - chr4_3:464830-464849 None:intergenic 40.0%
TACACTGAGGAAAATTGAGC+AGG - chr4_3:464852-464871 None:intergenic 40.0%
TGATGTGATGTCATACACTG+AGG - chr4_3:464865-464884 None:intergenic 40.0%
ACTATTCCCCATTTGCAAGA+TGG + chr4_3:464919-464938 Msa0623280:intron 40.0%
CACGATGTTCACAATTTCAG+GGG + chr4_3:465141-465160 Msa0623280:intron 40.0%
TGAATCCTTGCTCTTCAGAA+AGG - chr4_3:465193-465212 None:intergenic 40.0%
GTTAGCAGAACTCAATGCTT+TGG + chr4_3:465236-465255 Msa0623280:CDS 40.0%
!! AACATGCTGGAGGTATTAAC+AGG + chr4_3:465358-465377 Msa0623280:intron 40.0%
!! ACATGCTGGAGGTATTAACA+GGG + chr4_3:465359-465378 Msa0623280:intron 40.0%
!! CACAGCTGTTGATTTGAGTT+TGG + chr4_3:465406-465425 Msa0623280:CDS 40.0%
ACCAAAACTGTACCATCTAG+TGG + chr4_3:465430-465449 Msa0623280:CDS 40.0%
! ACCACTAGATGGTACAGTTT+TGG - chr4_3:465434-465453 None:intergenic 40.0%
AAAGGTCCACAACTACATCA+AGG + chr4_3:465499-465518 Msa0623280:CDS 40.0%
!! AACTGCATGATGGCATTGAT+TGG + chr4_3:465615-465634 Msa0623280:intron 40.0%
! TGCTTGGCAATGAGAACTTT+TGG + chr4_3:465638-465657 Msa0623280:intron 40.0%
GTCAACATAAAGTGTGTGGA+AGG + chr4_3:465775-465794 Msa0623280:intron 40.0%
TGGATGAAAGATGTATGGGA+AGG - chr4_3:465991-466010 None:intergenic 40.0%
!! GCATGCTTTTCTTATTTGCC+AGG + chr4_3:466357-466376 Msa0623280:intron 40.0%
GGATAATTAAGAGTGAACCC+TGG - chr4_3:466378-466397 None:intergenic 40.0%
TATCCTGCTTGACATGTACA+TGG + chr4_3:466393-466412 Msa0623280:intron 40.0%
! CTACTTCATGCATTGACCTT+TGG - chr4_3:467169-467188 None:intergenic 40.0%
! GTCTAAAGTTTGTCGCGATT+AGG - chr4_3:467522-467541 None:intergenic 40.0%
AGCTAAAGCTTACGGACTTA+CGG - chr4_3:467685-467704 None:intergenic 40.0%
ATCATCGCAAATGGCTCTTG+AGG + chr4_3:464378-464397 Msa0623280:exon 45.0%
ATATCTCCGACAAACCCCAA+TGG + chr4_3:464606-464625 Msa0623280:CDS 45.0%
! AGAGTACCATTGGGGTTTGT+CGG - chr4_3:464615-464634 None:intergenic 45.0%
GTTCCAGTGGTCAAGTTTGA+TGG + chr4_3:464753-464772 Msa0623280:CDS 45.0%
GGAGAAACAAGAGATGGTTC+AGG - chr4_3:464831-464850 None:intergenic 45.0%
TGAGCAGGAGAAACAAGAGA+TGG - chr4_3:464837-464856 None:intergenic 45.0%
TTTGTGGGATGAGGAATCTG+AGG + chr4_3:464975-464994 Msa0623280:intron 45.0%
TTGTGGGATGAGGAATCTGA+GGG + chr4_3:464976-464995 Msa0623280:intron 45.0%
TAACCACACTAACATGCTGG+AGG + chr4_3:465348-465367 Msa0623280:intron 45.0%
ATACCTCCAGCATGTTAGTG+TGG - chr4_3:465354-465373 None:intergenic 45.0%
! TAACAGGGTCCATTTGTTGC+TGG + chr4_3:465374-465393 Msa0623280:intron 45.0%
! AACAGGGTCCATTTGTTGCT+GGG + chr4_3:465375-465394 Msa0623280:intron 45.0%
TACCATCTAGTGGTAGCACT+TGG + chr4_3:465440-465459 Msa0623280:CDS 45.0%
CACTTGGTCACTTCAAGAGT+TGG + chr4_3:465456-465475 Msa0623280:CDS 45.0%
TGGACCTTTGCCAAATTCTC+AGG - chr4_3:465488-465507 None:intergenic 45.0%
CAGCTGTGAAAACTGCATGA+TGG + chr4_3:465605-465624 Msa0623280:intron 45.0%
!! TGATGGCATTGATTGGTGCT+TGG + chr4_3:465622-465641 Msa0623280:intron 45.0%
!! TATGGGTTTGCTATGCTTGC+TGG + chr4_3:465932-465951 Msa0623280:intron 45.0%
CTTCCATGTACATGTCAAGC+AGG - chr4_3:466399-466418 None:intergenic 45.0%
! AGGAGTATGTCATTGCTGGA+TGG + chr4_3:467140-467159 Msa0623280:CDS 45.0%
GGAGTATGTCATTGCTGGAT+GGG + chr4_3:467141-467160 Msa0623280:CDS 45.0%
! CCATGACACCCTTGTGTTTT+AGG - chr4_3:467440-467459 None:intergenic 45.0%
!! CCTAAAACACAAGGGTGTCA+TGG + chr4_3:467437-467456 Msa0623280:three_prime_UTR 45.0%
ACGATTCCAGATTCGAACCT+GGG - chr4_3:467746-467765 None:intergenic 45.0%
ATCATGTGGGAACTAGGCAA+AGG + chr4_3:467830-467849 Msa0623280:three_prime_UTR 45.0%
!! TTATTTATTTATTTATTTGT+AGG + chr4_3:464703-464722 Msa0623280:intron 5.0%
!! TTAAAAATTTAACAAAAATA+AGG - chr4_3:465309-465328 None:intergenic 5.0%
! GTTGGTGCTCCAACTGTTCT+CGG + chr4_3:464418-464437 Msa0623280:CDS 50.0%
GTACAGTCTCCGAGAACAGT+TGG - chr4_3:464430-464449 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - chr4_3:464452-464471 None:intergenic 50.0%
! CGTGACCTTTCTGAAGAGCA+AGG + chr4_3:465185-465204 Msa0623280:CDS 50.0%
! ACAGGGTCCATTTGTTGCTG+GGG + chr4_3:465376-465395 Msa0623280:intron 50.0%
! TCCATTTGTTGCTGGGGAGA+AGG + chr4_3:465382-465401 Msa0623280:CDS 50.0%
ACCTTCTCCCCAGCAACAAA+TGG - chr4_3:465386-465405 None:intergenic 50.0%
GACCAAGTGCTACCACTAGA+TGG - chr4_3:465445-465464 None:intergenic 50.0%
! GCAGAGGAGTATGTCATTGC+TGG + chr4_3:467136-467155 Msa0623280:CDS 50.0%
TGGACATTTCAGCCAGCCAA+TGG - chr4_3:467377-467396 None:intergenic 50.0%
!! AAACACAAGGGTGTCATGGC+AGG + chr4_3:467441-467460 Msa0623280:three_prime_UTR 50.0%
! TTAGGTTGCACCATTCACCC+AGG + chr4_3:467726-467745 Msa0623280:three_prime_UTR 50.0%
CAGATTCGAACCTGGGTGAA+TGG - chr4_3:467739-467758 None:intergenic 50.0%
CATTCACCCAGGTTCGAATC+TGG + chr4_3:467737-467756 Msa0623280:three_prime_UTR 50.0%
CACGATTCCAGATTCGAACC+TGG - chr4_3:467747-467766 None:intergenic 50.0%
TGTGGGAACTAGGCAAAGGT+TGG + chr4_3:467834-467853 Msa0623280:three_prime_UTR 50.0%
GGCTCTTGAGGTTGCTGTCA+AGG + chr4_3:464390-464409 Msa0623280:CDS 55.0%
GTTGCTGTCAAGGCTGCTGT+TGG + chr4_3:464400-464419 Msa0623280:CDS 55.0%
GGAAGTGAATCCTGGAGGGA+AGG + chr4_3:464731-464750 Msa0623280:CDS 55.0%
GAGGAATCTGAGGGAGACTG+TGG + chr4_3:464985-465004 Msa0623280:intron 55.0%
TGTGTACACCTGCACGCACA+AGG + chr4_3:466641-466660 Msa0623280:intron 55.0%
AGGGGAAGAAGACACTGCAG+TGG - chr4_3:466705-466724 None:intergenic 55.0%
CGAGAAAACCAAGGCTGCAG+AGG + chr4_3:467120-467139 Msa0623280:CDS 55.0%
GACATACTCCTCTGCAGCCT+TGG - chr4_3:467131-467150 None:intergenic 55.0%
CATTGCTGGATGGGCACCAA+AGG + chr4_3:467150-467169 Msa0623280:CDS 55.0%
GCTCGATCACAGTGCATGTG+TGG - chr4_3:467215-467234 None:intergenic 55.0%
TCCTGGAGGGAAGGTTCCAG+TGG + chr4_3:464740-464759 Msa0623280:CDS 60.0%
ACCACTGGAACCTTCCCTCC+AGG - chr4_3:464744-464763 None:intergenic 60.0%
!! GTTGATGGCCTTGTGCGTGC+AGG - chr4_3:466652-466671 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 464332 467911 464332 ID=Msa0623280;Name=Msa0623280
chr4_3 mRNA 464332 467911 464332 ID=Msa0623280-mRNA-1;Parent=Msa0623280;Name=Msa0623280-mRNA-1;_AED=0.08;_eAED=0.08;_QI=56|1|1|1|1|1|6|728|212
chr4_3 exon 464332 464445 464332 ID=Msa0623280-mRNA-1:exon:100;Parent=Msa0623280-mRNA-1
chr4_3 exon 464537 464627 464537 ID=Msa0623280-mRNA-1:exon:101;Parent=Msa0623280-mRNA-1
chr4_3 exon 464725 464868 464725 ID=Msa0623280-mRNA-1:exon:102;Parent=Msa0623280-mRNA-1
chr4_3 exon 465161 465278 465161 ID=Msa0623280-mRNA-1:exon:103;Parent=Msa0623280-mRNA-1
chr4_3 exon 465380 465520 465380 ID=Msa0623280-mRNA-1:exon:104;Parent=Msa0623280-mRNA-1
chr4_3 exon 467097 467911 467097 ID=Msa0623280-mRNA-1:exon:105;Parent=Msa0623280-mRNA-1
chr4_3 five_prime_UTR 464332 464387 464332 ID=Msa0623280-mRNA-1:five_prime_utr;Parent=Msa0623280-mRNA-1
chr4_3 CDS 464388 464445 464388 ID=Msa0623280-mRNA-1:cds;Parent=Msa0623280-mRNA-1
chr4_3 CDS 464537 464627 464537 ID=Msa0623280-mRNA-1:cds;Parent=Msa0623280-mRNA-1
chr4_3 CDS 464725 464868 464725 ID=Msa0623280-mRNA-1:cds;Parent=Msa0623280-mRNA-1
chr4_3 CDS 465161 465278 465161 ID=Msa0623280-mRNA-1:cds;Parent=Msa0623280-mRNA-1
chr4_3 CDS 465380 465520 465380 ID=Msa0623280-mRNA-1:cds;Parent=Msa0623280-mRNA-1
chr4_3 CDS 467097 467183 467097 ID=Msa0623280-mRNA-1:cds;Parent=Msa0623280-mRNA-1
chr4_3 three_prime_UTR 467184 467911 467184 ID=Msa0623280-mRNA-1:three_prime_utr;Parent=Msa0623280-mRNA-1
Gene Sequence

>Msa0623280

ATGGCTCTTGAGGTTGCTGTCAAGGCTGCTGTTGGTGCTCCAACTGTTCTCGGAGACTGTCCATTTTCCCAAAGGGTTCTCTTAACTTTGGAAGAGAAGAAGATTCCATACAACATCCATTTGATTAATATCTCCGACAAACCCCAATGGTTTTTGGAAGTGAATCCTGGAGGGAAGGTTCCAGTGGTCAAGTTTGATGGTAAATGGGTTCCTGATTCTGATGTTATTGTTGGAATTCTTGAAGACAAATACCCTGAACCATCTCTTGTTTCTCCTGCTCAATTTTCCTCAGTGGGATCAAATATATGGGATACTTTCGTGACCTTTCTGAAGAGCAAGGATTCAAATGATGGAACAGAACAAGCTTTGTTAGCAGAACTCAATGCTTTGGATGAACATCTTAAGGCCAATGGTCCATTTGTTGCTGGGGAGAAGGTCACAGCTGTTGATTTGAGTTTGGCACCAAAACTGTACCATCTAGTGGTAGCACTTGGTCACTTCAAGAGTTGGACTATTCCTGAGAATTTGGCAAAGGTCCACAACTACATCAAGTTGCTCTTCTCTCGTGAATCATTCGAGAAAACCAAGGCTGCAGAGGAGTATGTCATTGCTGGATGGGCACCAAAGGTCAATGCATGA
Protein sequence

>Msa0623280

MALEVAVKAAVGAPTVLGDCPFSQRVLLTLEEKKIPYNIHLINISDKPQWFLEVNPGGKVPVVKFDGKWVPDSDVIVGILEDKYPEPSLVSPAQFSSVGSNIWDTFVTFLKSKDSNDGTEQALLAELNALDEHLKANGPFVAGEKVTAVDLSLAPKLYHLVVALGHFKSWTIPENLAKVHNYIKLLFSRESFEKTKAAEEYVIAGWAPKVNA*