Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0634700 | KAF4346687.1 | 55.821 | 670 | 210 | 12 | 2 | 601 | 1 | 654 | 0.0 | 687 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0634700 | sp|F4HT21|IAN9_ARATH | 57.784 | 334 | 129 | 2 | 2 | 332 | 6 | 330 | 7.86e-124 | 372 |
| Msa0634700 | sp|F4HT21|IAN9_ARATH | 40.854 | 328 | 113 | 7 | 335 | 591 | 13 | 330 | 1.20e-53 | 189 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0634700 | A0A7J6DKP8 | 55.821 | 670 | 210 | 12 | 2 | 601 | 1 | 654 | 0.0 | 687 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0538930 | Msa0634700 | 0.830239 | 3.285380e-55 | -8.615850e-47 |
| Msa0607540 | Msa0634700 | 0.800894 | 1.163293e-48 | -8.615850e-47 |
| Msa0634700 | Msa0672570 | 0.830241 | 3.280729e-55 | -8.615850e-47 |
| Msa0634700 | Msa0673460 | 0.809317 | 2.008243e-50 | -8.615850e-47 |
| Msa0634700 | Msa0798860 | 0.823978 | 1.035035e-53 | -8.615850e-47 |
| Msa0634700 | Msa0979100 | 0.813299 | 2.746332e-51 | -8.615850e-47 |
| Msa0634700 | Msa1088950 | 0.800716 | 1.265156e-48 | -8.615850e-47 |
| Msa0634700 | Msa1143270 | 0.807861 | 4.110488e-50 | -8.615850e-47 |
| Msa0634700 | Msa1407060 | 0.808434 | 3.103332e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0634700 | MtrunA17_Chr4g0014101 | 97.647 | 340 | 4 | 1 | 1 | 336 | 1 | 340 | 0.0 | 669 |
| Msa0634700 | MtrunA17_Chr4g0014101 | 65.588 | 340 | 43 | 7 | 335 | 601 | 9 | 347 | 1.67e-122 | 365 |
| Msa0634700 | MtrunA17_Chr4g0014111 | 87.719 | 285 | 27 | 3 | 1 | 277 | 1 | 285 | 7.60e-174 | 495 |
| Msa0634700 | MtrunA17_Chr4g0014111 | 58.845 | 277 | 39 | 3 | 335 | 536 | 9 | 285 | 2.05e-95 | 295 |
| Msa0634700 | MtrunA17_Chr4g0014141 | 70.088 | 341 | 86 | 4 | 2 | 335 | 1 | 332 | 3.74e-168 | 482 |
| Msa0634700 | MtrunA17_Chr4g0014141 | 48.235 | 340 | 94 | 6 | 335 | 601 | 10 | 340 | 7.33e-89 | 278 |
| Msa0634700 | MtrunA17_Chr4g0014161 | 70.606 | 330 | 87 | 2 | 9 | 334 | 10 | 333 | 6.13e-163 | 469 |
| Msa0634700 | MtrunA17_Chr4g0014161 | 49.245 | 331 | 91 | 4 | 335 | 594 | 10 | 334 | 1.89e-89 | 281 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0634700 | AT1G33970.1 | 57.784 | 334 | 129 | 2 | 2 | 332 | 6 | 330 | 7.99e-125 | 372 |
| Msa0634700 | AT1G33970.1 | 40.854 | 328 | 113 | 7 | 335 | 591 | 13 | 330 | 1.22e-54 | 189 |
| Msa0634700 | AT1G33970.2 | 57.784 | 334 | 129 | 2 | 2 | 332 | 6 | 330 | 7.99e-125 | 372 |
| Msa0634700 | AT1G33970.2 | 40.854 | 328 | 113 | 7 | 335 | 591 | 13 | 330 | 1.22e-54 | 189 |
| Msa0634700 | AT1G33970.4 | 57.784 | 334 | 129 | 2 | 2 | 332 | 6 | 330 | 7.99e-125 | 372 |
| Msa0634700 | AT1G33970.4 | 40.854 | 328 | 113 | 7 | 335 | 591 | 13 | 330 | 1.22e-54 | 189 |
| Msa0634700 | AT1G33970.5 | 57.784 | 334 | 129 | 2 | 2 | 332 | 6 | 330 | 7.99e-125 | 372 |
| Msa0634700 | AT1G33970.5 | 40.854 | 328 | 113 | 7 | 335 | 591 | 13 | 330 | 1.22e-54 | 189 |
| Msa0634700 | AT1G33970.3 | 58.065 | 279 | 105 | 2 | 2 | 277 | 6 | 275 | 5.37e-103 | 313 |
| Msa0634700 | AT1G33970.3 | 42.238 | 277 | 113 | 7 | 335 | 588 | 13 | 265 | 4.05e-50 | 175 |
Find 139 sgRNAs with CRISPR-Local
Find 1012 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTAAGGAAGAATCTTG+AGG | 0.621998 | 4_3:+22338777 | Msa0634700:CDS |
| AAATAAGTGTGCAGTAGTCA+CGG | 0.567614 | 4_3:-22338947 | None:intergenic |
| AACATGATAGTATCACAGAA+TGG | 0.613239 | 4_3:+22326243 | Msa0634700:CDS |
| AACCATTACCACCTCGCTTG+CGG | 0.557199 | 4_3:-22338816 | None:intergenic |
| AACGATTCCCACACAAAGAG+AGG | 0.592811 | 4_3:-22326146 | None:intergenic |
| AAGCTATGTTGAAGATGATT+GGG | 0.476526 | 4_3:+22324133 | Msa0634700:CDS |
| AAGGACGAAAAGAAGCGATC+TGG | 0.516709 | 4_3:+22326192 | Msa0634700:CDS |
| AAGTCGAGGGCAAGCTCGTC+CGG | 0.440714 | 4_3:+22324287 | Msa0634700:CDS |
| AAGTTATGTTGAAGATGATT+GGG | 0.420774 | 4_3:+22334687 | Msa0634700:intron |
| AATGGCATGGATACCATCCT+TGG | 0.542900 | 4_3:-22325224 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACGATGATGATCAATGAT+TGG | - | chr4_3:22323328-22323347 | None:intergenic | 30.0% | |
| AATGAAACAGAATCGCTATA+GGG | - | chr4_3:22323400-22323419 | None:intergenic | 30.0% | |
| TAATGAAACAGAATCGCTAT+AGG | - | chr4_3:22323401-22323420 | None:intergenic | 30.0% | |
| TAGTAACTAGCTACAAATCA+TGG | - | chr4_3:22323656-22323675 | None:intergenic | 30.0% | |
| GAATCAAAAGCTATTAAGCA+CGG | - | chr4_3:22323863-22323882 | None:intergenic | 30.0% | |
| GAATCAAAAGCTATTAAGCA+CGG | - | chr4_3:22323863-22323882 | None:intergenic | 30.0% | |
| TATGATGACACTAAACAGTA+CGG | - | chr4_3:22324055-22324074 | None:intergenic | 30.0% | |
| AAGCTATGTTGAAGATGATT+GGG | + | chr4_3:22324133-22324152 | Msa0634700:CDS | 30.0% | |
| TTTCCCAAGAATACTATTAC+CGG | - | chr4_3:22324258-22324277 | None:intergenic | 30.0% | |
| TAATTTGGCATGTATTTACC+TGG | - | chr4_3:22324383-22324402 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 22323227 | 22339072 | 22323227 | ID=Msa0634700;Name=Msa0634700 |
| chr4_3 | mRNA | 22323227 | 22339072 | 22323227 | ID=Msa0634700-mRNA-1;Parent=Msa0634700;Name=Msa0634700-mRNA-1;_AED=0.13;_eAED=0.13;_QI=81|0.6|0.54|1|0.6|0.36|11|220|601 |
| chr4_3 | exon | 22323227 | 22323295 | 22323227 | ID=Msa0634700-mRNA-1:exon:5789;Parent=Msa0634700-mRNA-1 |
| chr4_3 | exon | 22324110 | 22324383 | 22324110 | ID=Msa0634700-mRNA-1:exon:5790;Parent=Msa0634700-mRNA-1 |
| chr4_3 | exon | 22325158 | 22325254 | 22325158 | ID=Msa0634700-mRNA-1:exon:5791;Parent=Msa0634700-mRNA-1 |
| chr4_3 | exon | 22325267 | 22325435 | 22325267 | ID=Msa0634700-mRNA-1:exon:5792;Parent=Msa0634700-mRNA-1 |
| chr4_3 | exon | 22326141 | 22326305 | 22326141 | ID=Msa0634700-mRNA-1:exon:5793;Parent=Msa0634700-mRNA-1 |
| chr4_3 | exon | 22326392 | 22326526 | 22326392 | ID=Msa0634700-mRNA-1:exon:5794;Parent=Msa0634700-mRNA-1 |
| chr4_3 | exon | 22328019 | 22328193 | 22328019 | ID=Msa0634700-mRNA-1:exon:5795;Parent=Msa0634700-mRNA-1 |
| chr4_3 | exon | 22334701 | 22334937 | 22334701 | ID=Msa0634700-mRNA-1:exon:5796;Parent=Msa0634700-mRNA-1 |
| chr4_3 | exon | 22335590 | 22335819 | 22335590 | ID=Msa0634700-mRNA-1:exon:5797;Parent=Msa0634700-mRNA-1 |
| chr4_3 | exon | 22336678 | 22336812 | 22336678 | ID=Msa0634700-mRNA-1:exon:5798;Parent=Msa0634700-mRNA-1 |
| chr4_3 | exon | 22338652 | 22339072 | 22338652 | ID=Msa0634700-mRNA-1:exon:5799;Parent=Msa0634700-mRNA-1 |
| chr4_3 | five_prime_UTR | 22323227 | 22323295 | 22323227 | ID=Msa0634700-mRNA-1:five_prime_utr;Parent=Msa0634700-mRNA-1 |
| chr4_3 | five_prime_UTR | 22324110 | 22324121 | 22324110 | ID=Msa0634700-mRNA-1:five_prime_utr;Parent=Msa0634700-mRNA-1 |
| chr4_3 | CDS | 22324122 | 22324383 | 22324122 | ID=Msa0634700-mRNA-1:cds;Parent=Msa0634700-mRNA-1 |
| chr4_3 | CDS | 22325158 | 22325254 | 22325158 | ID=Msa0634700-mRNA-1:cds;Parent=Msa0634700-mRNA-1 |
| chr4_3 | CDS | 22325267 | 22325435 | 22325267 | ID=Msa0634700-mRNA-1:cds;Parent=Msa0634700-mRNA-1 |
| chr4_3 | CDS | 22326141 | 22326305 | 22326141 | ID=Msa0634700-mRNA-1:cds;Parent=Msa0634700-mRNA-1 |
| chr4_3 | CDS | 22326392 | 22326526 | 22326392 | ID=Msa0634700-mRNA-1:cds;Parent=Msa0634700-mRNA-1 |
| chr4_3 | CDS | 22328019 | 22328193 | 22328019 | ID=Msa0634700-mRNA-1:cds;Parent=Msa0634700-mRNA-1 |
| chr4_3 | CDS | 22334701 | 22334937 | 22334701 | ID=Msa0634700-mRNA-1:cds;Parent=Msa0634700-mRNA-1 |
| chr4_3 | CDS | 22335590 | 22335819 | 22335590 | ID=Msa0634700-mRNA-1:cds;Parent=Msa0634700-mRNA-1 |
| chr4_3 | CDS | 22336678 | 22336812 | 22336678 | ID=Msa0634700-mRNA-1:cds;Parent=Msa0634700-mRNA-1 |
| chr4_3 | CDS | 22338652 | 22338852 | 22338652 | ID=Msa0634700-mRNA-1:cds;Parent=Msa0634700-mRNA-1 |
| chr4_3 | three_prime_UTR | 22338853 | 22339072 | 22338853 | ID=Msa0634700-mRNA-1:three_prime_utr;Parent=Msa0634700-mRNA-1 |
| Gene Sequence |
>Msa0634700 ATGATGGGGGGAAGCTATGTTGAAGATGATTGGGAATTGGCGTCGTCTGGATCAGCTAGCGAGGTTCAGATTTCAGCTAATGAAGCTCGGACAGTGGTTTTAGTTGGACGTACCGGCAATGGGAAGAGCGCGACCGGTAATAGTATTCTTGGGAAAAAGGTTTTTAAGTCGAGGGCAAGCTCGTCCGGTGTCACTTCCTCTTGTGAAATGCAGACTGCTGAGTTGAGTGATGGACAGATTGTCAATGTTATTGACACTCCAGGATTATTTGAAGTTTCTGCTGGATCTGAGTTTATTGGGAAAGAAATAGTCAAGTGCATAGACTTTGCCAAGGATGGTATCCATGCCATTCTTGTGGTATCACGATTTAGCGAGGAAGAAGAAAATGCTCTGCGCAGCTTGCAGACATTATTTGGAAGCAAGATTGTTGACTATATGATTGTGGTCTTCACGGGAGGGGATGAGTTAGAAGACGATGAAGATACACTTGACGATTATTTAGGCCGTGAATGTCCAGAGTCTTTGAAGCAAATCCTCTCTTTGTGTGGGAATCGTTGTGTTCTCTTTGACAACAAGACTAAGGACGAAAAGAAGCGATCTGGACAAGTTCAACAACTTCTCTCGTTTGTAAACATGATAGTATCACAGAATGGCGGGCAACCTTATACAGATGAGTTATTTGCAGAACTAAAGAAAGGAGCAATGAAACTGCATAGAGAACAAAGAAAGGTTGATTCCTTGGAAGGATATTCAGAAGGACAATTATCAGAGCTTAAAAAGCATATGCAGCAGACATATGAGGAGCAGCTAAAACATATTACTGAGATGATTGAGTCAAAGTTGAAGGAGGCAACTACAAGGCTTGAGAAACAATTGGCAGAAGAGCAAGCTGCAAGACTAAGAGCAGAAGACAGTGCAAAGTTGGCTCAAAAGAAATCAGACGATGAAATACGAAAACTAAGGAAGCATCTTGAGAAGGCGCATGAAGAACTCCGCAAGCGAGATGATTGGGAGTTAGCGTCGTCCGGATCAGTTAATGATGTTCATATTTCAGCTAATGAAGCTCGGACAGTGGTTTTGGTTGGCCGTACAGGCAATGGGAAAAGTGCAACCGGTAATAGTATTATTGGGAAACAGGTTTTTAGGTCGAGGGCTAGCTCGTCCGGTGTCACTTCCTCTTGTGAAATGCATGCTGCTGAGTTGAGTGATGGACAGATTGTCAATGTTATTGACACTCCAGTCAAGTGCATTGACTTTGCCAAGGATGGTATCCATGCCATCCTTGTGGTGTTATCTGTCAGATCACGGTTTAGCGAGGAAGAAGAAAATGCTCTGCGCAGCTTGCAGACATTATTTGGAAGCAAGATTGTTGACTATATGATCGTGGTCTTCACGGGAGGGGATGGACTAGAAGATGATCGAGATACACTTGCCGATTATTTAGGCGGTGAATGTCCAGAGTCTTTGAAGAAAGGAGCAATGGAACTGCATAGCGGGCAAAGAAAGATTGATTCCTTGGAAGGACATTCAAAAGAACAAATATCAGAGCTTAAAAAGCATATGCAGCAAACATACGAGGAGCAGCTAAAACATATTACTGAGACGATTGAGTCAAAGTTGAAGGAGTCAACTAAAAGGCTGGAGAAACAACTGAAAGAAGAGCATGCTGCAAGATTAAAAGCAGAAGACAATGCAAAGTTGGCTCAAAAGAAATCAGACAATGAAATACGAAAACTAAGGAAGAATCTTGAGGAGGCGCATGAAGAACTCCGCAAGCGAGGTGGTAATGGTTGTGCCATTCTATGA |
| Protein sequence |
>Msa0634700 MMGGSYVEDDWELASSGSASEVQISANEARTVVLVGRTGNGKSATGNSILGKKVFKSRASSSGVTSSCEMQTAELSDGQIVNVIDTPGLFEVSAGSEFIGKEIVKCIDFAKDGIHAILVVSRFSEEEENALRSLQTLFGSKIVDYMIVVFTGGDELEDDEDTLDDYLGRECPESLKQILSLCGNRCVLFDNKTKDEKKRSGQVQQLLSFVNMIVSQNGGQPYTDELFAELKKGAMKLHREQRKVDSLEGYSEGQLSELKKHMQQTYEEQLKHITEMIESKLKEATTRLEKQLAEEQAARLRAEDSAKLAQKKSDDEIRKLRKHLEKAHEELRKRDDWELASSGSVNDVHISANEARTVVLVGRTGNGKSATGNSIIGKQVFRSRASSSGVTSSCEMHAAELSDGQIVNVIDTPVKCIDFAKDGIHAILVVLSVRSRFSEEEENALRSLQTLFGSKIVDYMIVVFTGGDGLEDDRDTLADYLGGECPESLKKGAMELHSGQRKIDSLEGHSKEQISELKKHMQQTYEEQLKHITETIESKLKESTKRLEKQLKEEHAARLKAEDNAKLAQKKSDNEIRKLRKNLEEAHEELRKRGGNGCAIL* |
