AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1407060


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1407060 MtrunA17_Chr2g0330571 100.000 205 0 0 1 205 1 205 2.21e-150 415
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1407060 AT1G17730.1 87.805 205 23 1 1 205 1 203 1.09e-130 366
Msa1407060 AT1G73030.1 88.293 205 22 1 1 205 1 203 8.84e-119 335
Msa1407060 AT2G06530.2 27.273 165 116 2 19 180 7 170 6.62e-12 62.4
Msa1407060 AT2G06530.1 27.273 165 116 2 19 180 28 191 8.73e-12 62.4

Find 56 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAGGTGGCGGATGATTA+TGG 0.176951 tig0023257:+18838 Msa1407060:CDS
GGAGGTTCAAGCGGAGTTTA+TGG 0.198154 tig0023257:+18753 Msa1407060:CDS
CGAGAAGCAGTTTGTGAATA+TGG 0.254306 tig0023257:+18732 Msa1407060:CDS
GTCGACAATCAGCAAATCTA+TGG 0.272483 tig0023257:+18633 Msa1407060:CDS
TCATCTGCTCTGTGCGTTTA+CGG 0.305607 tig0023257:-18546 None:intergenic
GGAAGTTTCGGTTAATCTTC+CGG 0.355996 tig0023257:+18864 Msa1407060:CDS
TGATTATGGGCTGGAAGTTT+CGG 0.370615 tig0023257:+18852 Msa1407060:CDS
GGCGGCTGCTCATGCTGTTC+CGG 0.374454 tig0023257:+18888 Msa1407060:CDS
TGCTCATGCTGTTCCGGTTA+AGG 0.374890 tig0023257:+18894 Msa1407060:CDS
TCAGCATCCTTCTCCTTAAC+CGG 0.398938 tig0023257:-18907 None:intergenic
GAGCACTCACGGCGTCCAAT+CGG 0.402083 tig0023257:-18588 None:intergenic
CGTCCAATCGGGAGGATAAT+CGG 0.420046 tig0023257:-18576 None:intergenic
CGACATCTTCTGCAAATTCC+CGG 0.428245 tig0023257:-18692 None:intergenic
GGTTAATAGTTTGATGCAAC+AGG 0.440152 tig0023257:+18822 Msa1407060:CDS
AACAGGTGGCGGATGATTAT+GGG 0.452583 tig0023257:+18839 Msa1407060:CDS
TTCCCTTTCGACTCCGGAAG+GGG 0.455520 tig0023257:+18798 Msa1407060:CDS
TTCGGTTAATCTTCCGGTGG+CGG 0.462698 tig0023257:+18870 Msa1407060:CDS
ATCGACTTCCCTTTCGACTC+CGG 0.467684 tig0023257:+18792 Msa1407060:CDS
TTGGAATCGTCGTTGGCGAC+CGG 0.469029 tig0023257:+18673 Msa1407060:CDS
CTCCCCTTCCGGAGTCGAAA+GGG 0.473087 tig0023257:-18800 None:intergenic
AAGCTTCTCTGTGTTTCCCA+TGG 0.476337 tig0023257:-18368 None:intergenic
TAGTTTGATGCAACAGGTGG+CGG 0.477361 tig0023257:+18828 Msa1407060:CDS
AGCACTCACGGCGTCCAATC+GGG 0.483446 tig0023257:-18587 None:intergenic
ACAGCATGAGCAGCCGCCAC+CGG 0.500440 tig0023257:-18883 None:intergenic
TGGAATCGTCGTTGGCGACC+GGG 0.513207 tig0023257:+18674 Msa1407060:CDS
TGCTGTTCCGGTTAAGGAGA+AGG 0.523122 tig0023257:+18900 Msa1407060:CDS
CTTCCCTTTCGACTCCGGAA+GGG 0.524629 tig0023257:+18797 Msa1407060:CDS
AAACTATTAACCTCCCCTTC+CGG 0.527569 tig0023257:-18811 None:intergenic
GGGAAACATCGTGAAATCGT+TGG 0.530824 tig0023257:+18654 Msa1407060:CDS
CCAAGCACGAAAATGCGAAA+AGG 0.531908 tig0023257:+18438 Msa1407060:CDS
GAAGCTTCTGAATCAGATCA+TGG 0.534967 tig0023257:+18384 Msa1407060:CDS
ACTTCCCTTTCGACTCCGGA+AGG 0.549210 tig0023257:+18796 Msa1407060:CDS
GCAGAAGATGTCGGAGACGA+TGG 0.561045 tig0023257:+18702 Msa1407060:CDS
TAAGGAGAAGGATGCTGAGA+AGG 0.573843 tig0023257:+18912 Msa1407060:CDS
TCGACAATCAGCAAATCTAT+GGG 0.575428 tig0023257:+18634 Msa1407060:CDS
CCTCCCCTTCCGGAGTCGAA+AGG 0.580228 tig0023257:-18801 None:intergenic
GAAATCGTTGGAATCGTCGT+TGG 0.580522 tig0023257:+18666 Msa1407060:CDS
GGAGTTTATGGAAAGTGCTA+TGG 0.582336 tig0023257:+18765 Msa1407060:CDS
CCTTTCGACTCCGGAAGGGG+AGG 0.586411 tig0023257:+18801 Msa1407060:CDS
CTTGCTGAGCTTAAAGCTAG+AGG 0.589782 tig0023257:+18961 Msa1407060:CDS
GGTGGCGGATGATTATGGGC+TGG 0.595315 tig0023257:+18843 Msa1407060:CDS
GGATGCTGAGAAGGTTGATG+AGG 0.596267 tig0023257:+18921 Msa1407060:CDS
GTTTATGGAAAGTGCTATGG+CGG 0.596862 tig0023257:+18768 Msa1407060:CDS
TGTGAATATGGAGGTTCAAG+CGG 0.607620 tig0023257:+18744 Msa1407060:CDS
GAGTGCTCGTCTCGATACAC+AGG 0.608048 tig0023257:+18603 Msa1407060:CDS
AGCAATCGAGAAAGGTAACA+TGG 0.611081 tig0023257:+18492 Msa1407060:CDS
TGTATCGAGACGAGCACTCA+CGG 0.615716 tig0023257:-18599 None:intergenic
CGGGAATTTGCAGAAGATGT+CGG 0.620775 tig0023257:+18693 Msa1407060:CDS
CCTCCGATTATCCTCCCGAT+TGG 0.622929 tig0023257:+18573 Msa1407060:CDS
ATCGAGAAAGGTAACATGGA+CGG 0.631489 tig0023257:+18496 Msa1407060:CDS
TTGATGAGGATGATTTGTCA+AGG 0.632184 tig0023257:+18935 Msa1407060:CDS
TTTATGGAAAGTGCTATGGC+GGG 0.633565 tig0023257:+18769 Msa1407060:CDS
TAATAGTTTGATGCAACAGG+TGG 0.645930 tig0023257:+18825 Msa1407060:CDS
GAAGCAGTTTGTGAATATGG+AGG 0.657749 tig0023257:+18735 Msa1407060:CDS
AGTTTCGGTTAATCTTCCGG+TGG 0.683279 tig0023257:+18867 Msa1407060:CDS
CCAATCGGGAGGATAATCGG+AGG 0.739296 tig0023257:-18573 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCACCTTGAGTTTTT+CGG - tig0023257:18473-18492 None:intergenic 25.0%
AGAGAAATCCGAAAAACTCA+AGG + tig0023257:18462-18481 Msa1407060:CDS 35.0%
GTGAAAAAAGCAATCGAGAA+AGG + tig0023257:18484-18503 Msa1407060:CDS 35.0%
TCGACAATCAGCAAATCTAT+GGG + tig0023257:18634-18653 Msa1407060:CDS 35.0%
GGTTAATAGTTTGATGCAAC+AGG + tig0023257:18822-18841 Msa1407060:CDS 35.0%
TAATAGTTTGATGCAACAGG+TGG + tig0023257:18825-18844 Msa1407060:CDS 35.0%
! TTGATGAGGATGATTTGTCA+AGG + tig0023257:18935-18954 Msa1407060:CDS 35.0%
GAAGCTTCTGAATCAGATCA+TGG + tig0023257:18384-18403 Msa1407060:CDS 40.0%
!! TTGGCGTTGAAGAGATTTTG+AGG - tig0023257:18422-18441 None:intergenic 40.0%
AGCAATCGAGAAAGGTAACA+TGG + tig0023257:18492-18511 Msa1407060:CDS 40.0%
ATCGAGAAAGGTAACATGGA+CGG + tig0023257:18496-18515 Msa1407060:CDS 40.0%
GTCGACAATCAGCAAATCTA+TGG + tig0023257:18633-18652 Msa1407060:CDS 40.0%
CGAGAAGCAGTTTGTGAATA+TGG + tig0023257:18732-18751 Msa1407060:CDS 40.0%
GAAGCAGTTTGTGAATATGG+AGG + tig0023257:18735-18754 Msa1407060:CDS 40.0%
TGTGAATATGGAGGTTCAAG+CGG + tig0023257:18744-18763 Msa1407060:CDS 40.0%
!! GGAGTTTATGGAAAGTGCTA+TGG + tig0023257:18765-18784 Msa1407060:CDS 40.0%
!! GTTTATGGAAAGTGCTATGG+CGG + tig0023257:18768-18787 Msa1407060:CDS 40.0%
!! TTTATGGAAAGTGCTATGGC+GGG + tig0023257:18769-18788 Msa1407060:CDS 40.0%
AAACTATTAACCTCCCCTTC+CGG - tig0023257:18814-18833 None:intergenic 40.0%
!! TGATTATGGGCTGGAAGTTT+CGG + tig0023257:18852-18871 Msa1407060:CDS 40.0%
GGAAGTTTCGGTTAATCTTC+CGG + tig0023257:18864-18883 Msa1407060:CDS 40.0%
! CCTTTTCGCATTTTCGTGCT+TGG - tig0023257:18441-18460 None:intergenic 45.0%
CCAAGCACGAAAATGCGAAA+AGG + tig0023257:18438-18457 Msa1407060:CDS 45.0%
TCATCTGCTCTGTGCGTTTA+CGG - tig0023257:18549-18568 None:intergenic 45.0%
GGGAAACATCGTGAAATCGT+TGG + tig0023257:18654-18673 Msa1407060:CDS 45.0%
GAAATCGTTGGAATCGTCGT+TGG + tig0023257:18666-18685 Msa1407060:CDS 45.0%
CGACATCTTCTGCAAATTCC+CGG - tig0023257:18695-18714 None:intergenic 45.0%
CGGGAATTTGCAGAAGATGT+CGG + tig0023257:18693-18712 Msa1407060:CDS 45.0%
TAGTTTGATGCAACAGGTGG+CGG + tig0023257:18828-18847 Msa1407060:CDS 45.0%
!! AACAGGTGGCGGATGATTAT+GGG + tig0023257:18839-18858 Msa1407060:CDS 45.0%
AGTTTCGGTTAATCTTCCGG+TGG + tig0023257:18867-18886 Msa1407060:CDS 45.0%
TCAGCATCCTTCTCCTTAAC+CGG - tig0023257:18910-18929 None:intergenic 45.0%
TAAGGAGAAGGATGCTGAGA+AGG + tig0023257:18912-18931 Msa1407060:CDS 45.0%
CTTGCTGAGCTTAAAGCTAG+AGG + tig0023257:18961-18980 Msa1407060:CDS 45.0%
CGTCCAATCGGGAGGATAAT+CGG - tig0023257:18579-18598 None:intergenic 50.0%
TGTATCGAGACGAGCACTCA+CGG - tig0023257:18602-18621 None:intergenic 50.0%
GGAGGTTCAAGCGGAGTTTA+TGG + tig0023257:18753-18772 Msa1407060:CDS 50.0%
! ATCGACTTCCCTTTCGACTC+CGG + tig0023257:18792-18811 Msa1407060:CDS 50.0%
!! CAACAGGTGGCGGATGATTA+TGG + tig0023257:18838-18857 Msa1407060:CDS 50.0%
TTCGGTTAATCTTCCGGTGG+CGG + tig0023257:18870-18889 Msa1407060:CDS 50.0%
TGCTCATGCTGTTCCGGTTA+AGG + tig0023257:18894-18913 Msa1407060:CDS 50.0%
TGCTGTTCCGGTTAAGGAGA+AGG + tig0023257:18900-18919 Msa1407060:CDS 50.0%
!! GGATGCTGAGAAGGTTGATG+AGG + tig0023257:18921-18940 Msa1407060:CDS 50.0%
CCAATCGGGAGGATAATCGG+AGG - tig0023257:18576-18595 None:intergenic 55.0%
CCTCCGATTATCCTCCCGAT+TGG + tig0023257:18573-18592 Msa1407060:CDS 55.0%
GAGTGCTCGTCTCGATACAC+AGG + tig0023257:18603-18622 Msa1407060:CDS 55.0%
TTGGAATCGTCGTTGGCGAC+CGG + tig0023257:18673-18692 Msa1407060:CDS 55.0%
GCAGAAGATGTCGGAGACGA+TGG + tig0023257:18702-18721 Msa1407060:CDS 55.0%
ACTTCCCTTTCGACTCCGGA+AGG + tig0023257:18796-18815 Msa1407060:CDS 55.0%
CTTCCCTTTCGACTCCGGAA+GGG + tig0023257:18797-18816 Msa1407060:CDS 55.0%
TTCCCTTTCGACTCCGGAAG+GGG + tig0023257:18798-18817 Msa1407060:CDS 55.0%
AGCACTCACGGCGTCCAATC+GGG - tig0023257:18590-18609 None:intergenic 60.0%
GAGCACTCACGGCGTCCAAT+CGG - tig0023257:18591-18610 None:intergenic 60.0%
TGGAATCGTCGTTGGCGACC+GGG + tig0023257:18674-18693 Msa1407060:CDS 60.0%
CTCCCCTTCCGGAGTCGAAA+GGG - tig0023257:18803-18822 None:intergenic 60.0%
!! GGTGGCGGATGATTATGGGC+TGG + tig0023257:18843-18862 Msa1407060:CDS 60.0%
ACTCACGGCGTCCAATCGGG+AGG - tig0023257:18587-18606 None:intergenic 65.0%
CCTCCCCTTCCGGAGTCGAA+AGG - tig0023257:18804-18823 None:intergenic 65.0%
CCTTTCGACTCCGGAAGGGG+AGG + tig0023257:18801-18820 Msa1407060:CDS 65.0%
ACAGCATGAGCAGCCGCCAC+CGG - tig0023257:18886-18905 None:intergenic 65.0%
GGCGGCTGCTCATGCTGTTC+CGG + tig0023257:18888-18907 Msa1407060:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0023257 gene 18370 18987 18370 ID=Msa1407060;Name=Msa1407060
tig0023257 mRNA 18370 18987 18370 ID=Msa1407060-mRNA-1;Parent=Msa1407060;Name=Msa1407060-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|205
tig0023257 exon 18370 18987 18370 ID=Msa1407060-mRNA-1:exon:24864;Parent=Msa1407060-mRNA-1
tig0023257 CDS 18370 18987 18370 ID=Msa1407060-mRNA-1:cds;Parent=Msa1407060-mRNA-1
Gene Sequence

>Msa1407060

ATGGGAAACACAGAGAAGCTTCTGAATCAGATCATGGAATTGAAATTCACCTCAAAATCTCTTCAACGCCAAGCACGAAAATGCGAAAAGGAAGAGAAATCCGAAAAACTCAAGGTGAAAAAAGCAATCGAGAAAGGTAACATGGACGGTGCACGAATCTACGCTGAAAACGCGATCCGTAAACGCACAGAGCAGATGAACTACCTCCGATTATCCTCCCGATTGGACGCCGTGAGTGCTCGTCTCGATACACAGGCAAAGATGTCGACAATCAGCAAATCTATGGGAAACATCGTGAAATCGTTGGAATCGTCGTTGGCGACCGGGAATTTGCAGAAGATGTCGGAGACGATGGATCAATTCGAGAAGCAGTTTGTGAATATGGAGGTTCAAGCGGAGTTTATGGAAAGTGCTATGGCGGGATCGACTTCCCTTTCGACTCCGGAAGGGGAGGTTAATAGTTTGATGCAACAGGTGGCGGATGATTATGGGCTGGAAGTTTCGGTTAATCTTCCGGTGGCGGCTGCTCATGCTGTTCCGGTTAAGGAGAAGGATGCTGAGAAGGTTGATGAGGATGATTTGTCAAGGCGTCTTGCTGAGCTTAAAGCTAGAGGTTAA

Protein sequence

>Msa1407060

MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKSEKLKVKKAIEKGNMDGARIYAENAIRKRTEQMNYLRLSSRLDAVSARLDTQAKMSTISKSMGNIVKSLESSLATGNLQKMSETMDQFEKQFVNMEVQAEFMESAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVNLPVAAAHAVPVKEKDAEKVDEDDLSRRLAELKARG*