AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1088950


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1088950 MtrunA17_Chr2g0330571 100.000 205 0 0 1 205 1 205 2.21e-150 415
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1088950 AT1G17730.1 87.805 205 23 1 1 205 1 203 1.09e-130 366
Msa1088950 AT1G73030.1 88.293 205 22 1 1 205 1 203 8.84e-119 335
Msa1088950 AT2G06530.2 27.273 165 116 2 19 180 7 170 6.62e-12 62.4
Msa1088950 AT2G06530.1 27.273 165 116 2 19 180 28 191 8.73e-12 62.4

Find 56 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAGGTGGCGGATGATTA+TGG 0.176951 7_3:-2315225 Msa1088950:CDS
GGAGGTTCAAGCGGAGTTTA+TGG 0.198154 7_3:-2315310 Msa1088950:CDS
CGAGAAGCAGTTTGTGAATA+TGG 0.254306 7_3:-2315331 Msa1088950:CDS
GTCGACAATCAGCAAATCTA+TGG 0.272483 7_3:-2315430 Msa1088950:CDS
TCATCTGCTCTGTGCGTTTA+CGG 0.305607 7_3:+2315517 None:intergenic
GGAAGTTTCGGTTAATCTTC+CGG 0.355996 7_3:-2315199 Msa1088950:CDS
TGATTATGGGCTGGAAGTTT+CGG 0.370615 7_3:-2315211 Msa1088950:CDS
GGCGGCTGCTCATGCTGTTC+CGG 0.374454 7_3:-2315175 Msa1088950:CDS
TGCTCATGCTGTTCCGGTTA+AGG 0.374890 7_3:-2315169 Msa1088950:CDS
TCAGCATCCTTCTCCTTAAC+CGG 0.398938 7_3:+2315156 None:intergenic
GAGCACTCACGGCGTCCAAT+CGG 0.402083 7_3:+2315475 None:intergenic
CGTCCAATCGGGAGGATAAT+CGG 0.420046 7_3:+2315487 None:intergenic
CGACATCTTCTGCAAATTCC+CGG 0.428245 7_3:+2315371 None:intergenic
GGTTAATAGTTTGATGCAAC+AGG 0.440152 7_3:-2315241 Msa1088950:CDS
AACAGGTGGCGGATGATTAT+GGG 0.452583 7_3:-2315224 Msa1088950:CDS
TTCCCTTTCGACTCCGGAAG+GGG 0.455520 7_3:-2315265 Msa1088950:CDS
TTCGGTTAATCTTCCGGTGG+CGG 0.462698 7_3:-2315193 Msa1088950:CDS
ATCGACTTCCCTTTCGACTC+CGG 0.467684 7_3:-2315271 Msa1088950:CDS
TTGGAATCGTCGTTGGCGAC+CGG 0.469029 7_3:-2315390 Msa1088950:CDS
CTCCCCTTCCGGAGTCGAAA+GGG 0.473087 7_3:+2315263 None:intergenic
AAGCTTCTCTGTGTTTCCCA+TGG 0.476337 7_3:+2315695 None:intergenic
TAGTTTGATGCAACAGGTGG+CGG 0.477361 7_3:-2315235 Msa1088950:CDS
AGCACTCACGGCGTCCAATC+GGG 0.483446 7_3:+2315476 None:intergenic
ACAGCATGAGCAGCCGCCAC+CGG 0.500440 7_3:+2315180 None:intergenic
TGGAATCGTCGTTGGCGACC+GGG 0.513207 7_3:-2315389 Msa1088950:CDS
TGCTGTTCCGGTTAAGGAGA+AGG 0.523122 7_3:-2315163 Msa1088950:CDS
CTTCCCTTTCGACTCCGGAA+GGG 0.524629 7_3:-2315266 Msa1088950:CDS
AAACTATTAACCTCCCCTTC+CGG 0.527569 7_3:+2315252 None:intergenic
GGGAAACATCGTGAAATCGT+TGG 0.530824 7_3:-2315409 Msa1088950:CDS
CCAAGCACGAAAATGCGAAA+AGG 0.531908 7_3:-2315625 Msa1088950:CDS
GAAGCTTCTGAATCAGATCA+TGG 0.534967 7_3:-2315679 Msa1088950:CDS
ACTTCCCTTTCGACTCCGGA+AGG 0.549210 7_3:-2315267 Msa1088950:CDS
GCAGAAGATGTCGGAGACGA+TGG 0.561045 7_3:-2315361 Msa1088950:CDS
TAAGGAGAAGGATGCTGAGA+AGG 0.573843 7_3:-2315151 Msa1088950:CDS
TCGACAATCAGCAAATCTAT+GGG 0.575428 7_3:-2315429 Msa1088950:CDS
CCTCCCCTTCCGGAGTCGAA+AGG 0.580228 7_3:+2315262 None:intergenic
GAAATCGTTGGAATCGTCGT+TGG 0.580522 7_3:-2315397 Msa1088950:CDS
GGAGTTTATGGAAAGTGCTA+TGG 0.582336 7_3:-2315298 Msa1088950:CDS
CCTTTCGACTCCGGAAGGGG+AGG 0.586411 7_3:-2315262 Msa1088950:CDS
CTTGCTGAGCTTAAAGCTAG+AGG 0.589782 7_3:-2315102 Msa1088950:CDS
GGTGGCGGATGATTATGGGC+TGG 0.595315 7_3:-2315220 Msa1088950:CDS
GGATGCTGAGAAGGTTGATG+AGG 0.596267 7_3:-2315142 Msa1088950:CDS
GTTTATGGAAAGTGCTATGG+CGG 0.596862 7_3:-2315295 Msa1088950:CDS
TGTGAATATGGAGGTTCAAG+CGG 0.607620 7_3:-2315319 Msa1088950:CDS
GAGTGCTCGTCTCGATACAC+AGG 0.608048 7_3:-2315460 Msa1088950:CDS
AGCAATCGAGAAAGGTAACA+TGG 0.611081 7_3:-2315571 Msa1088950:CDS
TGTATCGAGACGAGCACTCA+CGG 0.615716 7_3:+2315464 None:intergenic
CGGGAATTTGCAGAAGATGT+CGG 0.620775 7_3:-2315370 Msa1088950:CDS
CCTCCGATTATCCTCCCGAT+TGG 0.622929 7_3:-2315490 Msa1088950:CDS
ATCGAGAAAGGTAACATGGA+CGG 0.631489 7_3:-2315567 Msa1088950:CDS
TTGATGAGGATGATTTGTCA+AGG 0.632184 7_3:-2315128 Msa1088950:CDS
TTTATGGAAAGTGCTATGGC+GGG 0.633565 7_3:-2315294 Msa1088950:CDS
TAATAGTTTGATGCAACAGG+TGG 0.645930 7_3:-2315238 Msa1088950:CDS
GAAGCAGTTTGTGAATATGG+AGG 0.657749 7_3:-2315328 Msa1088950:CDS
AGTTTCGGTTAATCTTCCGG+TGG 0.683279 7_3:-2315196 Msa1088950:CDS
CCAATCGGGAGGATAATCGG+AGG 0.739296 7_3:+2315490 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCACCTTGAGTTTTT+CGG + chr7_3:2315201-2315220 None:intergenic 25.0%
AGAGAAATCCGAAAAACTCA+AGG - chr7_3:2315190-2315209 Msa1088950:CDS 35.0%
GTGAAAAAAGCAATCGAGAA+AGG - chr7_3:2315212-2315231 Msa1088950:CDS 35.0%
TCGACAATCAGCAAATCTAT+GGG - chr7_3:2315362-2315381 Msa1088950:CDS 35.0%
GGTTAATAGTTTGATGCAAC+AGG - chr7_3:2315550-2315569 Msa1088950:CDS 35.0%
TAATAGTTTGATGCAACAGG+TGG - chr7_3:2315553-2315572 Msa1088950:CDS 35.0%
! TTGATGAGGATGATTTGTCA+AGG - chr7_3:2315663-2315682 Msa1088950:CDS 35.0%
GAAGCTTCTGAATCAGATCA+TGG - chr7_3:2315112-2315131 Msa1088950:CDS 40.0%
!! TTGGCGTTGAAGAGATTTTG+AGG + chr7_3:2315150-2315169 None:intergenic 40.0%
AGCAATCGAGAAAGGTAACA+TGG - chr7_3:2315220-2315239 Msa1088950:CDS 40.0%
ATCGAGAAAGGTAACATGGA+CGG - chr7_3:2315224-2315243 Msa1088950:CDS 40.0%
GTCGACAATCAGCAAATCTA+TGG - chr7_3:2315361-2315380 Msa1088950:CDS 40.0%
CGAGAAGCAGTTTGTGAATA+TGG - chr7_3:2315460-2315479 Msa1088950:CDS 40.0%
GAAGCAGTTTGTGAATATGG+AGG - chr7_3:2315463-2315482 Msa1088950:CDS 40.0%
TGTGAATATGGAGGTTCAAG+CGG - chr7_3:2315472-2315491 Msa1088950:CDS 40.0%
!! GGAGTTTATGGAAAGTGCTA+TGG - chr7_3:2315493-2315512 Msa1088950:CDS 40.0%
!! GTTTATGGAAAGTGCTATGG+CGG - chr7_3:2315496-2315515 Msa1088950:CDS 40.0%
!! TTTATGGAAAGTGCTATGGC+GGG - chr7_3:2315497-2315516 Msa1088950:CDS 40.0%
AAACTATTAACCTCCCCTTC+CGG + chr7_3:2315542-2315561 None:intergenic 40.0%
!! TGATTATGGGCTGGAAGTTT+CGG - chr7_3:2315580-2315599 Msa1088950:CDS 40.0%
GGAAGTTTCGGTTAATCTTC+CGG - chr7_3:2315592-2315611 Msa1088950:CDS 40.0%
! CCTTTTCGCATTTTCGTGCT+TGG + chr7_3:2315169-2315188 None:intergenic 45.0%
CCAAGCACGAAAATGCGAAA+AGG - chr7_3:2315166-2315185 Msa1088950:CDS 45.0%
TCATCTGCTCTGTGCGTTTA+CGG + chr7_3:2315277-2315296 None:intergenic 45.0%
GGGAAACATCGTGAAATCGT+TGG - chr7_3:2315382-2315401 Msa1088950:CDS 45.0%
GAAATCGTTGGAATCGTCGT+TGG - chr7_3:2315394-2315413 Msa1088950:CDS 45.0%
CGACATCTTCTGCAAATTCC+CGG + chr7_3:2315423-2315442 None:intergenic 45.0%
CGGGAATTTGCAGAAGATGT+CGG - chr7_3:2315421-2315440 Msa1088950:CDS 45.0%
TAGTTTGATGCAACAGGTGG+CGG - chr7_3:2315556-2315575 Msa1088950:CDS 45.0%
!! AACAGGTGGCGGATGATTAT+GGG - chr7_3:2315567-2315586 Msa1088950:CDS 45.0%
AGTTTCGGTTAATCTTCCGG+TGG - chr7_3:2315595-2315614 Msa1088950:CDS 45.0%
TCAGCATCCTTCTCCTTAAC+CGG + chr7_3:2315638-2315657 None:intergenic 45.0%
TAAGGAGAAGGATGCTGAGA+AGG - chr7_3:2315640-2315659 Msa1088950:CDS 45.0%
CTTGCTGAGCTTAAAGCTAG+AGG - chr7_3:2315689-2315708 Msa1088950:CDS 45.0%
CGTCCAATCGGGAGGATAAT+CGG + chr7_3:2315307-2315326 None:intergenic 50.0%
TGTATCGAGACGAGCACTCA+CGG + chr7_3:2315330-2315349 None:intergenic 50.0%
GGAGGTTCAAGCGGAGTTTA+TGG - chr7_3:2315481-2315500 Msa1088950:CDS 50.0%
! ATCGACTTCCCTTTCGACTC+CGG - chr7_3:2315520-2315539 Msa1088950:CDS 50.0%
!! CAACAGGTGGCGGATGATTA+TGG - chr7_3:2315566-2315585 Msa1088950:CDS 50.0%
TTCGGTTAATCTTCCGGTGG+CGG - chr7_3:2315598-2315617 Msa1088950:CDS 50.0%
TGCTCATGCTGTTCCGGTTA+AGG - chr7_3:2315622-2315641 Msa1088950:CDS 50.0%
TGCTGTTCCGGTTAAGGAGA+AGG - chr7_3:2315628-2315647 Msa1088950:CDS 50.0%
!! GGATGCTGAGAAGGTTGATG+AGG - chr7_3:2315649-2315668 Msa1088950:CDS 50.0%
CCAATCGGGAGGATAATCGG+AGG + chr7_3:2315304-2315323 None:intergenic 55.0%
CCTCCGATTATCCTCCCGAT+TGG - chr7_3:2315301-2315320 Msa1088950:CDS 55.0%
GAGTGCTCGTCTCGATACAC+AGG - chr7_3:2315331-2315350 Msa1088950:CDS 55.0%
TTGGAATCGTCGTTGGCGAC+CGG - chr7_3:2315401-2315420 Msa1088950:CDS 55.0%
GCAGAAGATGTCGGAGACGA+TGG - chr7_3:2315430-2315449 Msa1088950:CDS 55.0%
ACTTCCCTTTCGACTCCGGA+AGG - chr7_3:2315524-2315543 Msa1088950:CDS 55.0%
CTTCCCTTTCGACTCCGGAA+GGG - chr7_3:2315525-2315544 Msa1088950:CDS 55.0%
TTCCCTTTCGACTCCGGAAG+GGG - chr7_3:2315526-2315545 Msa1088950:CDS 55.0%
AGCACTCACGGCGTCCAATC+GGG + chr7_3:2315318-2315337 None:intergenic 60.0%
GAGCACTCACGGCGTCCAAT+CGG + chr7_3:2315319-2315338 None:intergenic 60.0%
TGGAATCGTCGTTGGCGACC+GGG - chr7_3:2315402-2315421 Msa1088950:CDS 60.0%
CTCCCCTTCCGGAGTCGAAA+GGG + chr7_3:2315531-2315550 None:intergenic 60.0%
!! GGTGGCGGATGATTATGGGC+TGG - chr7_3:2315571-2315590 Msa1088950:CDS 60.0%
ACTCACGGCGTCCAATCGGG+AGG + chr7_3:2315315-2315334 None:intergenic 65.0%
CCTCCCCTTCCGGAGTCGAA+AGG + chr7_3:2315532-2315551 None:intergenic 65.0%
CCTTTCGACTCCGGAAGGGG+AGG - chr7_3:2315529-2315548 Msa1088950:CDS 65.0%
ACAGCATGAGCAGCCGCCAC+CGG + chr7_3:2315614-2315633 None:intergenic 65.0%
GGCGGCTGCTCATGCTGTTC+CGG - chr7_3:2315616-2315635 Msa1088950:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 2315098 2315715 2315098 ID=Msa1088950;Name=Msa1088950
chr7_3 mRNA 2315098 2315715 2315098 ID=Msa1088950-mRNA-1;Parent=Msa1088950;Name=Msa1088950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|205
chr7_3 exon 2315098 2315715 2315098 ID=Msa1088950-mRNA-1:exon:943;Parent=Msa1088950-mRNA-1
chr7_3 CDS 2315098 2315715 2315098 ID=Msa1088950-mRNA-1:cds;Parent=Msa1088950-mRNA-1
Gene Sequence

>Msa1088950

ATGGGAAACACAGAGAAGCTTCTGAATCAGATCATGGAATTGAAATTCACCTCAAAATCTCTTCAACGCCAAGCACGAAAATGCGAAAAGGAAGAGAAATCCGAAAAACTCAAGGTGAAAAAAGCAATCGAGAAAGGTAACATGGACGGTGCACGAATCTACGCTGAAAACGCGATCCGTAAACGCACAGAGCAGATGAACTACCTCCGATTATCCTCCCGATTGGACGCCGTGAGTGCTCGTCTCGATACACAGGCAAAGATGTCGACAATCAGCAAATCTATGGGAAACATCGTGAAATCGTTGGAATCGTCGTTGGCGACCGGGAATTTGCAGAAGATGTCGGAGACGATGGATCAATTCGAGAAGCAGTTTGTGAATATGGAGGTTCAAGCGGAGTTTATGGAAAGTGCTATGGCGGGATCGACTTCCCTTTCGACTCCGGAAGGGGAGGTTAATAGTTTGATGCAACAGGTGGCGGATGATTATGGGCTGGAAGTTTCGGTTAATCTTCCGGTGGCGGCTGCTCATGCTGTTCCGGTTAAGGAGAAGGATGCTGAGAAGGTTGATGAGGATGATTTGTCAAGGCGTCTTGCTGAGCTTAAAGCTAGAGGTTAA

Protein sequence

>Msa1088950

MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKSEKLKVKKAIEKGNMDGARIYAENAIRKRTEQMNYLRLSSRLDAVSARLDTQAKMSTISKSMGNIVKSLESSLATGNLQKMSETMDQFEKQFVNMEVQAEFMESAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVNLPVAAAHAVPVKEKDAEKVDEDDLSRRLAELKARG*