AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0295330


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0295330 MtrunA17_Chr2g0317561 99.306 144 1 0 21 164 1 144 2.84e-103 292
Msa0295330 MtrunA17_Chr1g0197981 99.306 144 1 0 21 164 1 144 4.71e-103 291
Msa0295330 MtrunA17_Chr2g0282251 67.606 142 46 0 22 163 3 144 4.87e-67 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0295330 AT1G53035.1 61.806 144 55 0 21 164 7 150 9.19e-63 190
Msa0295330 AT1G53035.2 61.111 144 56 0 21 164 7 150 4.42e-62 189
Msa0295330 AT3G15358.1 60.417 144 57 0 21 164 1 144 3.87e-60 183

Find 35 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTGCTTTGGCTATTTCT+TGG 0.186754 2_3:+47881231 Msa0295330:CDS
GAAGAAAGCAATTGAAATTA+TGG 0.218565 2_3:-47880513 None:intergenic
TTAAGACTTCAAAGTGGTTT+TGG 0.231225 2_3:+47880834 Msa0295330:CDS
GGATTATTGTTGTTCACAAT+TGG 0.247863 2_3:+47881047 Msa0295330:CDS
AAAGTATGGAGATTGGAATT+TGG 0.289357 2_3:+47880596 Msa0295330:exon
CAAACATCAGATTCCTTCTC+TGG 0.317012 2_3:+47881026 Msa0295330:CDS
TCTGAAGAGGTACATGTCTT+AGG 0.336826 2_3:+47880942 Msa0295330:CDS
GGTTGCTTGTGTTAAGGATA+TGG 0.337831 2_3:+47881085 Msa0295330:CDS
TCTTGCTTTGGCTATTTCTT+GGG 0.340551 2_3:+47881232 Msa0295330:CDS
GATTATTGTTGTTCACAATT+GGG 0.349528 2_3:+47881048 Msa0295330:CDS
TTCATATTTCAATGGCAGTT+TGG 0.351079 2_3:+47881144 Msa0295330:CDS
ATTGGAATTTGGATGGTAAC+AGG 0.356802 2_3:+47880607 Msa0295330:CDS
AATTGGGTTTCTTGTGTTCA+TGG 0.359752 2_3:+47881064 Msa0295330:CDS
TGTTGTTGGGGATCTTGCTT+TGG 0.362898 2_3:+47881220 Msa0295330:CDS
ATTGGCCTAGGCATGTTGTT+GGG 0.364725 2_3:+47881207 Msa0295330:CDS
TTTAACATGTGGATCATTGA+TGG 0.365683 2_3:+47880911 Msa0295330:CDS
AGGTAGCAACCACAAAAGTA+TGG 0.392478 2_3:+47880582 Msa0295330:exon
TTTCAAAGTTTCTTTGCTAA+AGG 0.405302 2_3:+47881110 Msa0295330:CDS
TTGAAGAACTTGCTTGTGAT+TGG 0.405372 2_3:+47881189 Msa0295330:CDS
ATTGCTTAGTTCAACAAGAA+AGG 0.440472 2_3:+47880562 Msa0295330:five_prime_UTR
GTTCATGGTTGCTTGTGTTA+AGG 0.446942 2_3:+47881079 Msa0295330:CDS
TGTGTTTCTTCATATTTCAA+TGG 0.458849 2_3:+47881136 Msa0295330:CDS
AACTTGCTTGTGATTGGCCT+AGG 0.494396 2_3:+47881195 Msa0295330:CDS
GATTGGCCTAGGCATGTTGT+TGG 0.498297 2_3:+47881206 Msa0295330:CDS
AACCACAAAAGTATGGAGAT+TGG 0.499882 2_3:+47880589 Msa0295330:exon
TATGGAGATTGGAATTTGGA+TGG 0.527537 2_3:+47880600 Msa0295330:exon
TTGGCCTAGGCATGTTGTTG+GGG 0.550117 2_3:+47881208 Msa0295330:CDS
AAGACAGCAATAAAGCTACA+AGG 0.572726 2_3:+47880975 Msa0295330:CDS
AGATCCCCAACAACATGCCT+AGG 0.575697 2_3:-47881212 None:intergenic
TCAACAATAGCTTTAACATG+TGG 0.588889 2_3:+47880900 Msa0295330:CDS
GAGGTACATGTCTTAGGACA+TGG 0.605633 2_3:+47880948 Msa0295330:CDS
GAACAACAATAATCCAGAGA+AGG 0.611024 2_3:-47881039 None:intergenic
GAAACTTTAAGACTTCAAAG+TGG 0.622567 2_3:+47880828 Msa0295330:intron
TGAATTAATAACTGATCATG+TGG 0.652499 2_3:-47881005 None:intergenic
AGACAGCAATAAAGCTACAA+GGG 0.673413 2_3:+47880976 Msa0295330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAATTTGAAGTGTTATG+AGG + chr2_3:47880799-47880818 Msa0295330:intron 25.0%
! TGAATTAATAACTGATCATG+TGG - chr2_3:47881008-47881027 None:intergenic 25.0%
!! GATTATTGTTGTTCACAATT+GGG + chr2_3:47881048-47881067 Msa0295330:CDS 25.0%
! TTTCAAAGTTTCTTTGCTAA+AGG + chr2_3:47881110-47881129 Msa0295330:CDS 25.0%
! TGTGTTTCTTCATATTTCAA+TGG + chr2_3:47881136-47881155 Msa0295330:CDS 25.0%
!! TTTGGAGATTTTACTTTGTA+GGG + chr2_3:47881162-47881181 Msa0295330:CDS 25.0%
!!! TGTTTTTTCTTGTGTTTATG+TGG + chr2_3:47881255-47881274 Msa0295330:CDS 25.0%
!! ATGAAATTTGAAGTGTTATG+AGG + chr2_3:47880799-47880818 Msa0295330:intron 25.0%
! TGAATTAATAACTGATCATG+TGG - chr2_3:47881008-47881027 None:intergenic 25.0%
!! GATTATTGTTGTTCACAATT+GGG + chr2_3:47881048-47881067 Msa0295330:CDS 25.0%
! TTTCAAAGTTTCTTTGCTAA+AGG + chr2_3:47881110-47881129 Msa0295330:CDS 25.0%
! TGTGTTTCTTCATATTTCAA+TGG + chr2_3:47881136-47881155 Msa0295330:CDS 25.0%
!! TTTGGAGATTTTACTTTGTA+GGG + chr2_3:47881162-47881181 Msa0295330:CDS 25.0%
!!! TGTTTTTTCTTGTGTTTATG+TGG + chr2_3:47881255-47881274 Msa0295330:CDS 25.0%
ATTGCTTAGTTCAACAAGAA+AGG + chr2_3:47880562-47880581 Msa0295330:five_prime_UTR 30.0%
AAAGTATGGAGATTGGAATT+TGG + chr2_3:47880596-47880615 Msa0295330:exon 30.0%
CACAAGTATTTGTAGTTCAA+AGG + chr2_3:47880740-47880759 Msa0295330:intron 30.0%
!! GAAACTTTAAGACTTCAAAG+TGG + chr2_3:47880828-47880847 Msa0295330:intron 30.0%
! TTAAGACTTCAAAGTGGTTT+TGG + chr2_3:47880834-47880853 Msa0295330:CDS 30.0%
!!! TCAAAGTGGTTTTGGAAAAA+TGG + chr2_3:47880842-47880861 Msa0295330:CDS 30.0%
!!! CAAAGTGGTTTTGGAAAAAT+GGG + chr2_3:47880843-47880862 Msa0295330:CDS 30.0%
!! TCAACAATAGCTTTAACATG+TGG + chr2_3:47880900-47880919 Msa0295330:CDS 30.0%
TTTAACATGTGGATCATTGA+TGG + chr2_3:47880911-47880930 Msa0295330:CDS 30.0%
! GGATTATTGTTGTTCACAAT+TGG + chr2_3:47881047-47881066 Msa0295330:CDS 30.0%
TTCATATTTCAATGGCAGTT+TGG + chr2_3:47881144-47881163 Msa0295330:CDS 30.0%
! GTTTGGAGATTTTACTTTGT+AGG + chr2_3:47881161-47881180 Msa0295330:CDS 30.0%
! TTTTTCTTGTGTTTATGTGG+AGG + chr2_3:47881258-47881277 Msa0295330:CDS 30.0%
! TTTTCTTGTGTTTATGTGGA+GGG + chr2_3:47881259-47881278 Msa0295330:CDS 30.0%
ATTGCTTAGTTCAACAAGAA+AGG + chr2_3:47880562-47880581 Msa0295330:five_prime_UTR 30.0%
AAAGTATGGAGATTGGAATT+TGG + chr2_3:47880596-47880615 Msa0295330:exon 30.0%
CACAAGTATTTGTAGTTCAA+AGG + chr2_3:47880740-47880759 Msa0295330:intron 30.0%
!! GAAACTTTAAGACTTCAAAG+TGG + chr2_3:47880828-47880847 Msa0295330:intron 30.0%
! TTAAGACTTCAAAGTGGTTT+TGG + chr2_3:47880834-47880853 Msa0295330:CDS 30.0%
!!! TCAAAGTGGTTTTGGAAAAA+TGG + chr2_3:47880842-47880861 Msa0295330:CDS 30.0%
!!! CAAAGTGGTTTTGGAAAAAT+GGG + chr2_3:47880843-47880862 Msa0295330:CDS 30.0%
!! TCAACAATAGCTTTAACATG+TGG + chr2_3:47880900-47880919 Msa0295330:CDS 30.0%
TTTAACATGTGGATCATTGA+TGG + chr2_3:47880911-47880930 Msa0295330:CDS 30.0%
! GGATTATTGTTGTTCACAAT+TGG + chr2_3:47881047-47881066 Msa0295330:CDS 30.0%
TTCATATTTCAATGGCAGTT+TGG + chr2_3:47881144-47881163 Msa0295330:CDS 30.0%
! GTTTGGAGATTTTACTTTGT+AGG + chr2_3:47881161-47881180 Msa0295330:CDS 30.0%
! TTTTTCTTGTGTTTATGTGG+AGG + chr2_3:47881258-47881277 Msa0295330:CDS 30.0%
! TTTTCTTGTGTTTATGTGGA+GGG + chr2_3:47881259-47881278 Msa0295330:CDS 30.0%
! AACCACAAAAGTATGGAGAT+TGG + chr2_3:47880589-47880608 Msa0295330:exon 35.0%
! TTCCAATCTCCATACTTTTG+TGG - chr2_3:47880594-47880613 None:intergenic 35.0%
TATGGAGATTGGAATTTGGA+TGG + chr2_3:47880600-47880619 Msa0295330:exon 35.0%
ATTGGAATTTGGATGGTAAC+AGG + chr2_3:47880607-47880626 Msa0295330:CDS 35.0%
AATTTGGATGGTAACAGGTA+TGG + chr2_3:47880612-47880631 Msa0295330:intron 35.0%
AAAAGGAGATGAATCAGAAG+AGG - chr2_3:47880709-47880728 None:intergenic 35.0%
CTTGTGAAAGAAGTCTGAAA+AGG - chr2_3:47880726-47880745 None:intergenic 35.0%
!!! GATGGTTTTTTACTCTGAAG+AGG + chr2_3:47880929-47880948 Msa0295330:CDS 35.0%
AAGACAGCAATAAAGCTACA+AGG + chr2_3:47880975-47880994 Msa0295330:CDS 35.0%
AGACAGCAATAAAGCTACAA+GGG + chr2_3:47880976-47880995 Msa0295330:CDS 35.0%
GAACAACAATAATCCAGAGA+AGG - chr2_3:47881042-47881061 None:intergenic 35.0%
AATTGGGTTTCTTGTGTTCA+TGG + chr2_3:47881064-47881083 Msa0295330:CDS 35.0%
! GAGATTTTACTTTGTAGGGA+AGG + chr2_3:47881166-47881185 Msa0295330:CDS 35.0%
! TTGAAGAACTTGCTTGTGAT+TGG + chr2_3:47881189-47881208 Msa0295330:CDS 35.0%
!! ATCTTGCTTTGGCTATTTCT+TGG + chr2_3:47881231-47881250 Msa0295330:CDS 35.0%
!! TCTTGCTTTGGCTATTTCTT+GGG + chr2_3:47881232-47881251 Msa0295330:CDS 35.0%
! AACCACAAAAGTATGGAGAT+TGG + chr2_3:47880589-47880608 Msa0295330:exon 35.0%
! TTCCAATCTCCATACTTTTG+TGG - chr2_3:47880594-47880613 None:intergenic 35.0%
TATGGAGATTGGAATTTGGA+TGG + chr2_3:47880600-47880619 Msa0295330:exon 35.0%
ATTGGAATTTGGATGGTAAC+AGG + chr2_3:47880607-47880626 Msa0295330:CDS 35.0%
AATTTGGATGGTAACAGGTA+TGG + chr2_3:47880612-47880631 Msa0295330:intron 35.0%
AAAAGGAGATGAATCAGAAG+AGG - chr2_3:47880709-47880728 None:intergenic 35.0%
CTTGTGAAAGAAGTCTGAAA+AGG - chr2_3:47880726-47880745 None:intergenic 35.0%
!!! GATGGTTTTTTACTCTGAAG+AGG + chr2_3:47880929-47880948 Msa0295330:CDS 35.0%
AAGACAGCAATAAAGCTACA+AGG + chr2_3:47880975-47880994 Msa0295330:CDS 35.0%
AGACAGCAATAAAGCTACAA+GGG + chr2_3:47880976-47880995 Msa0295330:CDS 35.0%
GAACAACAATAATCCAGAGA+AGG - chr2_3:47881042-47881061 None:intergenic 35.0%
AATTGGGTTTCTTGTGTTCA+TGG + chr2_3:47881064-47881083 Msa0295330:CDS 35.0%
! GAGATTTTACTTTGTAGGGA+AGG + chr2_3:47881166-47881185 Msa0295330:CDS 35.0%
! TTGAAGAACTTGCTTGTGAT+TGG + chr2_3:47881189-47881208 Msa0295330:CDS 35.0%
!! ATCTTGCTTTGGCTATTTCT+TGG + chr2_3:47881231-47881250 Msa0295330:CDS 35.0%
!! TCTTGCTTTGGCTATTTCTT+GGG + chr2_3:47881232-47881251 Msa0295330:CDS 35.0%
AGGTAGCAACCACAAAAGTA+TGG + chr2_3:47880582-47880601 Msa0295330:exon 40.0%
! TCTGAAGAGGTACATGTCTT+AGG + chr2_3:47880942-47880961 Msa0295330:CDS 40.0%
CAAACATCAGATTCCTTCTC+TGG + chr2_3:47881026-47881045 Msa0295330:CDS 40.0%
GTTCATGGTTGCTTGTGTTA+AGG + chr2_3:47881079-47881098 Msa0295330:CDS 40.0%
GGTTGCTTGTGTTAAGGATA+TGG + chr2_3:47881085-47881104 Msa0295330:CDS 40.0%
AGGTAGCAACCACAAAAGTA+TGG + chr2_3:47880582-47880601 Msa0295330:exon 40.0%
! TCTGAAGAGGTACATGTCTT+AGG + chr2_3:47880942-47880961 Msa0295330:CDS 40.0%
CAAACATCAGATTCCTTCTC+TGG + chr2_3:47881026-47881045 Msa0295330:CDS 40.0%
GTTCATGGTTGCTTGTGTTA+AGG + chr2_3:47881079-47881098 Msa0295330:CDS 40.0%
GGTTGCTTGTGTTAAGGATA+TGG + chr2_3:47881085-47881104 Msa0295330:CDS 40.0%
GAGGTACATGTCTTAGGACA+TGG + chr2_3:47880948-47880967 Msa0295330:CDS 45.0%
!! AACTTGCTTGTGATTGGCCT+AGG + chr2_3:47881195-47881214 Msa0295330:CDS 45.0%
! ATTGGCCTAGGCATGTTGTT+GGG + chr2_3:47881207-47881226 Msa0295330:CDS 45.0%
TGTTGTTGGGGATCTTGCTT+TGG + chr2_3:47881220-47881239 Msa0295330:CDS 45.0%
GAGGTACATGTCTTAGGACA+TGG + chr2_3:47880948-47880967 Msa0295330:CDS 45.0%
!! AACTTGCTTGTGATTGGCCT+AGG + chr2_3:47881195-47881214 Msa0295330:CDS 45.0%
! ATTGGCCTAGGCATGTTGTT+GGG + chr2_3:47881207-47881226 Msa0295330:CDS 45.0%
TGTTGTTGGGGATCTTGCTT+TGG + chr2_3:47881220-47881239 Msa0295330:CDS 45.0%
!! GATTGGCCTAGGCATGTTGT+TGG + chr2_3:47881206-47881225 Msa0295330:CDS 50.0%
TTGGCCTAGGCATGTTGTTG+GGG + chr2_3:47881208-47881227 Msa0295330:CDS 50.0%
AGATCCCCAACAACATGCCT+AGG - chr2_3:47881215-47881234 None:intergenic 50.0%
!! GATTGGCCTAGGCATGTTGT+TGG + chr2_3:47881206-47881225 Msa0295330:CDS 50.0%
TTGGCCTAGGCATGTTGTTG+GGG + chr2_3:47881208-47881227 Msa0295330:CDS 50.0%
AGATCCCCAACAACATGCCT+AGG - chr2_3:47881215-47881234 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 47880514 47881295 47880514 ID=Msa0295330;Name=Msa0295330
chr2_3 mRNA 47880514 47881295 47880514 ID=Msa0295330-mRNA-1;Parent=Msa0295330;Name=Msa0295330-mRNA-1;_AED=0.16;_eAED=0.21;_QI=87|0|0.5|1|0|0|2|0|164
chr2_3 exon 47880514 47880628 47880514 ID=Msa0295330-mRNA-1:exon:14928;Parent=Msa0295330-mRNA-1
chr2_3 exon 47880829 47881295 47880829 ID=Msa0295330-mRNA-1:exon:14929;Parent=Msa0295330-mRNA-1
chr2_3 five_prime_UTR 47880514 47880600 47880514 ID=Msa0295330-mRNA-1:five_prime_utr;Parent=Msa0295330-mRNA-1
chr2_3 CDS 47880601 47880628 47880601 ID=Msa0295330-mRNA-1:cds;Parent=Msa0295330-mRNA-1
chr2_3 CDS 47880829 47881295 47880829 ID=Msa0295330-mRNA-1:cds;Parent=Msa0295330-mRNA-1
Gene Sequence

>Msa0295330

ATGGAGATTGGAATTTGGATGGTAACAGAAACTTTAAGACTTCAAAGTGGTTTTGGAAAAATGGGTTCATCTTGTTTTTTCTTAATATGTGTTCTTCATTCAACAATAGCTTTAACATGTGGATCATTGATGGTTTTTTACTCTGAAGAGGTACATGTCTTAGGACATGGAACAAAGACAGCAATAAAGCTACAAGGGTCAACACCACATGATCAGTTATTAATTCAAACATCAGATTCCTTCTCTGGATTATTGTTGTTCACAATTGGGTTTCTTGTGTTCATGGTTGCTTGTGTTAAGGATATGGAGTTTCAAAGTTTCTTTGCTAAAGGATGTGTGTTTCTTCATATTTCAATGGCAGTTTGGAGATTTTACTTTGTAGGGAAGGTTGAAGAACTTGCTTGTGATTGGCCTAGGCATGTTGTTGGGGATCTTGCTTTGGCTATTTCTTGGGTGTTTTTTCTTGTGTTTATGTGGAGGGAGAAATATGATTAG

Protein sequence

>Msa0295330

MEIGIWMVTETLRLQSGFGKMGSSCFFLICVLHSTIALTCGSLMVFYSEEVHVLGHGTKTAIKLQGSTPHDQLLIQTSDSFSGLLLFTIGFLVFMVACVKDMEFQSFFAKGCVFLHISMAVWRFYFVGKVEELACDWPRHVVGDLALAISWVFFLVFMWREKYD*