AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0793380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0793380 MtrunA17_Chr2g0317561 98.611 144 2 0 21 164 1 144 1.26e-102 290
Msa0793380 MtrunA17_Chr1g0197981 98.611 144 2 0 21 164 1 144 1.74e-102 290
Msa0793380 MtrunA17_Chr2g0282251 67.606 142 46 0 22 163 3 144 3.08e-67 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0793380 AT1G53035.1 61.806 144 55 0 21 164 7 150 9.60e-63 190
Msa0793380 AT1G53035.2 61.111 144 56 0 21 164 7 150 4.83e-62 189
Msa0793380 AT3G15358.1 60.417 144 57 0 21 164 1 144 3.67e-60 183

Find 35 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTGCTTTGGCTATTTCT+TGG 0.186754 5_3:+5361882 Msa0793380:CDS
GAAGAAAGCAATTGAAATTA+TGG 0.218565 5_3:-5361164 None:intergenic
TTAAGACTTCAAAGTGGTTT+TGG 0.231225 5_3:+5361485 Msa0793380:CDS
GGATTATTGTTGTTCACAAT+TGG 0.247863 5_3:+5361698 Msa0793380:CDS
AAAGTATGGAGATTGGAATT+TGG 0.289357 5_3:+5361247 Msa0793380:exon
CAAACATCAGATTCCTTCTC+TGG 0.317012 5_3:+5361677 Msa0793380:CDS
TCTGAAGAGGTACATGTCTT+AGG 0.336826 5_3:+5361593 Msa0793380:CDS
GGTTGCTTGTGTTAAGGATA+TGG 0.337831 5_3:+5361736 Msa0793380:CDS
TCTTGCTTTGGCTATTTCTT+GGG 0.340551 5_3:+5361883 Msa0793380:CDS
GATTATTGTTGTTCACAATT+GGG 0.349528 5_3:+5361699 Msa0793380:CDS
TTCATATTTCAATGGCAGTT+TGG 0.351079 5_3:+5361795 Msa0793380:CDS
ATTGGAATTTGGATGGTAAC+AGG 0.356802 5_3:+5361258 Msa0793380:CDS
AATTGGGTTTCTTGTGTTCA+TGG 0.359752 5_3:+5361715 Msa0793380:CDS
TTTAACATGTGGATCATTGA+TGG 0.365683 5_3:+5361562 Msa0793380:CDS
TGTTGTTGGGGATCTTGCTT+TGG 0.372557 5_3:+5361871 Msa0793380:CDS
AGGTAGCAACCACAAAAGTA+TGG 0.392478 5_3:+5361233 Msa0793380:exon
ATTGGCCTAGGAATGTTGTT+GGG 0.401238 5_3:+5361858 Msa0793380:CDS
TTTCAAAGTTTCTTTGCTAA+AGG 0.405302 5_3:+5361761 Msa0793380:CDS
TTGAAGAACTTGCTTGTGAT+TGG 0.405372 5_3:+5361840 Msa0793380:CDS
ATTGCTTAGTTCAACAAGAA+AGG 0.440472 5_3:+5361213 Msa0793380:five_prime_UTR
GTTCATGGTTGCTTGTGTTA+AGG 0.446942 5_3:+5361730 Msa0793380:CDS
TGTGTTTCTTCATATTTCAA+TGG 0.458849 5_3:+5361787 Msa0793380:CDS
AACTTGCTTGTGATTGGCCT+AGG 0.490660 5_3:+5361846 Msa0793380:CDS
AACCACAAAAGTATGGAGAT+TGG 0.499882 5_3:+5361240 Msa0793380:exon
AGATCCCCAACAACATTCCT+AGG 0.500133 5_3:-5361863 None:intergenic
GATTGGCCTAGGAATGTTGT+TGG 0.503249 5_3:+5361857 Msa0793380:CDS
TATGGAGATTGGAATTTGGA+TGG 0.527537 5_3:+5361251 Msa0793380:exon
AAGACAGCAATAAAGCTACA+AGG 0.572726 5_3:+5361626 Msa0793380:CDS
TCAACAATAGCTTTAACATG+TGG 0.588889 5_3:+5361551 Msa0793380:CDS
TTGGCCTAGGAATGTTGTTG+GGG 0.595854 5_3:+5361859 Msa0793380:CDS
GAGGTACATGTCTTAGGACA+TGG 0.605633 5_3:+5361599 Msa0793380:CDS
GAACAACAATAATCCAGAGA+AGG 0.611024 5_3:-5361690 None:intergenic
GAAACTTTAAGACTTCAAAG+TGG 0.622567 5_3:+5361479 Msa0793380:intron
TGAATTAATAACTGATCATG+TGG 0.652499 5_3:-5361656 None:intergenic
AGACAGCAATAAAGCTACAA+GGG 0.673413 5_3:+5361627 Msa0793380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CACAAGTATTTGTAATTCAA+AGG + chr5_3:5361391-5361410 Msa0793380:intron 25.0%
!! ATGAAATTTGAAGTGTTATG+AGG + chr5_3:5361450-5361469 Msa0793380:intron 25.0%
! TGAATTAATAACTGATCATG+TGG - chr5_3:5361659-5361678 None:intergenic 25.0%
!! GATTATTGTTGTTCACAATT+GGG + chr5_3:5361699-5361718 Msa0793380:CDS 25.0%
! TTTCAAAGTTTCTTTGCTAA+AGG + chr5_3:5361761-5361780 Msa0793380:CDS 25.0%
! TGTGTTTCTTCATATTTCAA+TGG + chr5_3:5361787-5361806 Msa0793380:CDS 25.0%
!! TTTGGAGATTTTACTTTGTA+GGG + chr5_3:5361813-5361832 Msa0793380:CDS 25.0%
!!! TGTTTTTTCTTGTGTTTATG+TGG + chr5_3:5361906-5361925 Msa0793380:CDS 25.0%
! CACAAGTATTTGTAATTCAA+AGG + chr5_3:5361391-5361410 Msa0793380:intron 25.0%
!! ATGAAATTTGAAGTGTTATG+AGG + chr5_3:5361450-5361469 Msa0793380:intron 25.0%
! TGAATTAATAACTGATCATG+TGG - chr5_3:5361659-5361678 None:intergenic 25.0%
!! GATTATTGTTGTTCACAATT+GGG + chr5_3:5361699-5361718 Msa0793380:CDS 25.0%
! TTTCAAAGTTTCTTTGCTAA+AGG + chr5_3:5361761-5361780 Msa0793380:CDS 25.0%
! TGTGTTTCTTCATATTTCAA+TGG + chr5_3:5361787-5361806 Msa0793380:CDS 25.0%
!! TTTGGAGATTTTACTTTGTA+GGG + chr5_3:5361813-5361832 Msa0793380:CDS 25.0%
!!! TGTTTTTTCTTGTGTTTATG+TGG + chr5_3:5361906-5361925 Msa0793380:CDS 25.0%
ATTGCTTAGTTCAACAAGAA+AGG + chr5_3:5361213-5361232 Msa0793380:five_prime_UTR 30.0%
AAAGTATGGAGATTGGAATT+TGG + chr5_3:5361247-5361266 Msa0793380:exon 30.0%
!! GAAACTTTAAGACTTCAAAG+TGG + chr5_3:5361479-5361498 Msa0793380:intron 30.0%
! TTAAGACTTCAAAGTGGTTT+TGG + chr5_3:5361485-5361504 Msa0793380:CDS 30.0%
!!! TCAAAGTGGTTTTGGAAAAA+TGG + chr5_3:5361493-5361512 Msa0793380:CDS 30.0%
!!! CAAAGTGGTTTTGGAAAAAT+GGG + chr5_3:5361494-5361513 Msa0793380:CDS 30.0%
!! TCAACAATAGCTTTAACATG+TGG + chr5_3:5361551-5361570 Msa0793380:CDS 30.0%
TTTAACATGTGGATCATTGA+TGG + chr5_3:5361562-5361581 Msa0793380:CDS 30.0%
! GGATTATTGTTGTTCACAAT+TGG + chr5_3:5361698-5361717 Msa0793380:CDS 30.0%
TTCATATTTCAATGGCAGTT+TGG + chr5_3:5361795-5361814 Msa0793380:CDS 30.0%
! GTTTGGAGATTTTACTTTGT+AGG + chr5_3:5361812-5361831 Msa0793380:CDS 30.0%
! TTTTTCTTGTGTTTATGTGG+AGG + chr5_3:5361909-5361928 Msa0793380:CDS 30.0%
! TTTTCTTGTGTTTATGTGGA+GGG + chr5_3:5361910-5361929 Msa0793380:CDS 30.0%
ATTGCTTAGTTCAACAAGAA+AGG + chr5_3:5361213-5361232 Msa0793380:five_prime_UTR 30.0%
AAAGTATGGAGATTGGAATT+TGG + chr5_3:5361247-5361266 Msa0793380:exon 30.0%
!! GAAACTTTAAGACTTCAAAG+TGG + chr5_3:5361479-5361498 Msa0793380:intron 30.0%
! TTAAGACTTCAAAGTGGTTT+TGG + chr5_3:5361485-5361504 Msa0793380:CDS 30.0%
!!! TCAAAGTGGTTTTGGAAAAA+TGG + chr5_3:5361493-5361512 Msa0793380:CDS 30.0%
!!! CAAAGTGGTTTTGGAAAAAT+GGG + chr5_3:5361494-5361513 Msa0793380:CDS 30.0%
!! TCAACAATAGCTTTAACATG+TGG + chr5_3:5361551-5361570 Msa0793380:CDS 30.0%
TTTAACATGTGGATCATTGA+TGG + chr5_3:5361562-5361581 Msa0793380:CDS 30.0%
! GGATTATTGTTGTTCACAAT+TGG + chr5_3:5361698-5361717 Msa0793380:CDS 30.0%
TTCATATTTCAATGGCAGTT+TGG + chr5_3:5361795-5361814 Msa0793380:CDS 30.0%
! GTTTGGAGATTTTACTTTGT+AGG + chr5_3:5361812-5361831 Msa0793380:CDS 30.0%
! TTTTTCTTGTGTTTATGTGG+AGG + chr5_3:5361909-5361928 Msa0793380:CDS 30.0%
! TTTTCTTGTGTTTATGTGGA+GGG + chr5_3:5361910-5361929 Msa0793380:CDS 30.0%
! AACCACAAAAGTATGGAGAT+TGG + chr5_3:5361240-5361259 Msa0793380:exon 35.0%
! TTCCAATCTCCATACTTTTG+TGG - chr5_3:5361245-5361264 None:intergenic 35.0%
TATGGAGATTGGAATTTGGA+TGG + chr5_3:5361251-5361270 Msa0793380:exon 35.0%
ATTGGAATTTGGATGGTAAC+AGG + chr5_3:5361258-5361277 Msa0793380:CDS 35.0%
AATTTGGATGGTAACAGGTA+TGG + chr5_3:5361263-5361282 Msa0793380:intron 35.0%
AAAAGGAGATGAATCAGAAG+AGG - chr5_3:5361360-5361379 None:intergenic 35.0%
CTTGTGAAAGAAGTCTGAAA+AGG - chr5_3:5361377-5361396 None:intergenic 35.0%
!!! GATGGTTTTTTACTCTGAAG+AGG + chr5_3:5361580-5361599 Msa0793380:CDS 35.0%
AAGACAGCAATAAAGCTACA+AGG + chr5_3:5361626-5361645 Msa0793380:CDS 35.0%
AGACAGCAATAAAGCTACAA+GGG + chr5_3:5361627-5361646 Msa0793380:CDS 35.0%
GAACAACAATAATCCAGAGA+AGG - chr5_3:5361693-5361712 None:intergenic 35.0%
AATTGGGTTTCTTGTGTTCA+TGG + chr5_3:5361715-5361734 Msa0793380:CDS 35.0%
! GAGATTTTACTTTGTAGGGA+AGG + chr5_3:5361817-5361836 Msa0793380:CDS 35.0%
! TTGAAGAACTTGCTTGTGAT+TGG + chr5_3:5361840-5361859 Msa0793380:CDS 35.0%
!! ATCTTGCTTTGGCTATTTCT+TGG + chr5_3:5361882-5361901 Msa0793380:CDS 35.0%
!! TCTTGCTTTGGCTATTTCTT+GGG + chr5_3:5361883-5361902 Msa0793380:CDS 35.0%
! AACCACAAAAGTATGGAGAT+TGG + chr5_3:5361240-5361259 Msa0793380:exon 35.0%
! TTCCAATCTCCATACTTTTG+TGG - chr5_3:5361245-5361264 None:intergenic 35.0%
TATGGAGATTGGAATTTGGA+TGG + chr5_3:5361251-5361270 Msa0793380:exon 35.0%
ATTGGAATTTGGATGGTAAC+AGG + chr5_3:5361258-5361277 Msa0793380:CDS 35.0%
AATTTGGATGGTAACAGGTA+TGG + chr5_3:5361263-5361282 Msa0793380:intron 35.0%
AAAAGGAGATGAATCAGAAG+AGG - chr5_3:5361360-5361379 None:intergenic 35.0%
CTTGTGAAAGAAGTCTGAAA+AGG - chr5_3:5361377-5361396 None:intergenic 35.0%
!!! GATGGTTTTTTACTCTGAAG+AGG + chr5_3:5361580-5361599 Msa0793380:CDS 35.0%
AAGACAGCAATAAAGCTACA+AGG + chr5_3:5361626-5361645 Msa0793380:CDS 35.0%
AGACAGCAATAAAGCTACAA+GGG + chr5_3:5361627-5361646 Msa0793380:CDS 35.0%
GAACAACAATAATCCAGAGA+AGG - chr5_3:5361693-5361712 None:intergenic 35.0%
AATTGGGTTTCTTGTGTTCA+TGG + chr5_3:5361715-5361734 Msa0793380:CDS 35.0%
! GAGATTTTACTTTGTAGGGA+AGG + chr5_3:5361817-5361836 Msa0793380:CDS 35.0%
! TTGAAGAACTTGCTTGTGAT+TGG + chr5_3:5361840-5361859 Msa0793380:CDS 35.0%
!! ATCTTGCTTTGGCTATTTCT+TGG + chr5_3:5361882-5361901 Msa0793380:CDS 35.0%
!! TCTTGCTTTGGCTATTTCTT+GGG + chr5_3:5361883-5361902 Msa0793380:CDS 35.0%
AGGTAGCAACCACAAAAGTA+TGG + chr5_3:5361233-5361252 Msa0793380:exon 40.0%
! TCTGAAGAGGTACATGTCTT+AGG + chr5_3:5361593-5361612 Msa0793380:CDS 40.0%
CAAACATCAGATTCCTTCTC+TGG + chr5_3:5361677-5361696 Msa0793380:CDS 40.0%
GTTCATGGTTGCTTGTGTTA+AGG + chr5_3:5361730-5361749 Msa0793380:CDS 40.0%
GGTTGCTTGTGTTAAGGATA+TGG + chr5_3:5361736-5361755 Msa0793380:CDS 40.0%
! ATTGGCCTAGGAATGTTGTT+GGG + chr5_3:5361858-5361877 Msa0793380:CDS 40.0%
AGGTAGCAACCACAAAAGTA+TGG + chr5_3:5361233-5361252 Msa0793380:exon 40.0%
! TCTGAAGAGGTACATGTCTT+AGG + chr5_3:5361593-5361612 Msa0793380:CDS 40.0%
CAAACATCAGATTCCTTCTC+TGG + chr5_3:5361677-5361696 Msa0793380:CDS 40.0%
GTTCATGGTTGCTTGTGTTA+AGG + chr5_3:5361730-5361749 Msa0793380:CDS 40.0%
GGTTGCTTGTGTTAAGGATA+TGG + chr5_3:5361736-5361755 Msa0793380:CDS 40.0%
! ATTGGCCTAGGAATGTTGTT+GGG + chr5_3:5361858-5361877 Msa0793380:CDS 40.0%
GAGGTACATGTCTTAGGACA+TGG + chr5_3:5361599-5361618 Msa0793380:CDS 45.0%
!! AACTTGCTTGTGATTGGCCT+AGG + chr5_3:5361846-5361865 Msa0793380:CDS 45.0%
!! GATTGGCCTAGGAATGTTGT+TGG + chr5_3:5361857-5361876 Msa0793380:CDS 45.0%
TTGGCCTAGGAATGTTGTTG+GGG + chr5_3:5361859-5361878 Msa0793380:CDS 45.0%
AGATCCCCAACAACATTCCT+AGG - chr5_3:5361866-5361885 None:intergenic 45.0%
TGTTGTTGGGGATCTTGCTT+TGG + chr5_3:5361871-5361890 Msa0793380:CDS 45.0%
GAGGTACATGTCTTAGGACA+TGG + chr5_3:5361599-5361618 Msa0793380:CDS 45.0%
!! AACTTGCTTGTGATTGGCCT+AGG + chr5_3:5361846-5361865 Msa0793380:CDS 45.0%
!! GATTGGCCTAGGAATGTTGT+TGG + chr5_3:5361857-5361876 Msa0793380:CDS 45.0%
TTGGCCTAGGAATGTTGTTG+GGG + chr5_3:5361859-5361878 Msa0793380:CDS 45.0%
AGATCCCCAACAACATTCCT+AGG - chr5_3:5361866-5361885 None:intergenic 45.0%
TGTTGTTGGGGATCTTGCTT+TGG + chr5_3:5361871-5361890 Msa0793380:CDS 45.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 5361165 5361946 5361165 ID=Msa0793380;Name=Msa0793380
chr5_3 mRNA 5361165 5361946 5361165 ID=Msa0793380-mRNA-1;Parent=Msa0793380;Name=Msa0793380-mRNA-1;_AED=0.16;_eAED=0.21;_QI=87|0|0.5|1|0|0|2|0|164
chr5_3 exon 5361165 5361279 5361165 ID=Msa0793380-mRNA-1:exon:2049;Parent=Msa0793380-mRNA-1
chr5_3 exon 5361480 5361946 5361480 ID=Msa0793380-mRNA-1:exon:2050;Parent=Msa0793380-mRNA-1
chr5_3 five_prime_UTR 5361165 5361251 5361165 ID=Msa0793380-mRNA-1:five_prime_utr;Parent=Msa0793380-mRNA-1
chr5_3 CDS 5361252 5361279 5361252 ID=Msa0793380-mRNA-1:cds;Parent=Msa0793380-mRNA-1
chr5_3 CDS 5361480 5361946 5361480 ID=Msa0793380-mRNA-1:cds;Parent=Msa0793380-mRNA-1
Gene Sequence

>Msa0793380

ATGGAGATTGGAATTTGGATGGTAACAGAAACTTTAAGACTTCAAAGTGGTTTTGGAAAAATGGGTTCATCTTGTTTTTTCTTAATATGTGTTCTTCATTCAACAATAGCTTTAACATGTGGATCATTGATGGTTTTTTACTCTGAAGAGGTACATGTCTTAGGACATGGAACAAAGACAGCAATAAAGCTACAAGGGTCAACACCACATGATCAGTTATTAATTCAAACATCAGATTCCTTCTCTGGATTATTGTTGTTCACAATTGGGTTTCTTGTGTTCATGGTTGCTTGTGTTAAGGATATGGAGTTTCAAAGTTTCTTTGCTAAAGGATGTGTGTTTCTTCATATTTCAATGGCAGTTTGGAGATTTTACTTTGTAGGGAAGGTTGAAGAACTTGCTTGTGATTGGCCTAGGAATGTTGTTGGGGATCTTGCTTTGGCTATTTCTTGGGTGTTTTTTCTTGTGTTTATGTGGAGGGAGAAATATGATTAG

Protein sequence

>Msa0793380

MEIGIWMVTETLRLQSGFGKMGSSCFFLICVLHSTIALTCGSLMVFYSEEVHVLGHGTKTAIKLQGSTPHDQLLIQTSDSFSGLLLFTIGFLVFMVACVKDMEFQSFFAKGCVFLHISMAVWRFYFVGKVEELACDWPRNVVGDLALAISWVFFLVFMWREKYD*