AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1215330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1215330 MtrunA17_Chr4g0064691 97.041 169 5 0 1 169 1 169 3.01e-109 308
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTTCTTTGCTGGTGGC+TGG 0.201684 8_1:+76565489 None:intergenic
TCTCACTTCCTCTTCTTTGC+TGG 0.250916 8_1:+76565482 None:intergenic
TTCCCTTTGGAGCACCTTCT+TGG 0.276978 8_1:-76565791 Msa1215330:CDS
CCTCTTCTTTGCTGGTGGCT+GGG 0.297534 8_1:+76565490 None:intergenic
TTGTGTCAAGCTTAGTGATT+AGG 0.324884 8_1:-76565423 Msa1215330:three_prime_UTR
CTCTTCTTTGCTGGTGGCTG+GGG 0.325642 8_1:+76565491 None:intergenic
GAATTATATTAGTAGGAAAT+TGG 0.326206 8_1:-76565461 Msa1215330:three_prime_UTR
CAACTTTGGAGCCACTGATT+TGG 0.351405 8_1:+76565914 None:intergenic
AGAGAATTGGAAGGTGAGAT+TGG 0.354776 8_1:+76566952 None:intergenic
CACTCACGGACAATAATAAC+TGG 0.374970 8_1:+76565295 None:intergenic
AATCCTTGTCAGGTTCCCTT+TGG 0.384520 8_1:-76565804 Msa1215330:intron
TGACACAGGTTCACTTACTA+AGG 0.409062 8_1:+76565384 None:intergenic
ACTTGAGTTGAGTTGAGTCC+AGG 0.410529 8_1:-76567067 Msa1215330:five_prime_UTR
GAATCCATTGCAGTCAACTT+TGG 0.411321 8_1:+76565900 None:intergenic
CACTTCCTCTTCTTTGCTGG+TGG 0.417470 8_1:+76565485 None:intergenic
TTCTTCCTCGCCTTCATCAC+CGG 0.419016 8_1:+76565658 None:intergenic
ATTGCTCGTGCTATCAATCT+TGG 0.420153 8_1:-76565241 Msa1215330:three_prime_UTR
GAGGAGTCAACAACAACAAT+AGG 0.424929 8_1:+76567005 None:intergenic
AGAGGACGAAGAGGAGGAAG+AGG 0.432417 8_1:-76565528 Msa1215330:CDS
TTAGTAGGAAATTGGTTATG+TGG 0.455585 8_1:-76565453 Msa1215330:three_prime_UTR
AGGTCGACGACGGAGAGAAT+TGG 0.458122 8_1:+76566939 None:intergenic
TGAGGATGATGATGGAGAAG+AGG 0.459842 8_1:-76565690 Msa1215330:CDS
GAAGTGAGAATTATATTAGT+AGG 0.468566 8_1:-76565468 Msa1215330:exon
GATCCTGAGGCTAACGGTGC+AGG 0.476679 8_1:-76565620 Msa1215330:CDS
GTCAACAACAACAATAGGTT+TGG 0.480887 8_1:+76567010 None:intergenic
GGTGATGAAGGCGAGGAAGA+AGG 0.491443 8_1:-76565656 Msa1215330:CDS
GAGAATTGGAAGGTGAGATT+GGG 0.491676 8_1:+76566953 None:intergenic
GAAGACTACTCCGGTGATGA+AGG 0.492863 8_1:-76565668 Msa1215330:CDS
CTTTGGAGCACCTTCTTGGA+AGG 0.496704 8_1:-76565787 Msa1215330:CDS
CTCTCTCTCACTCTCGGCAA+TGG 0.497209 8_1:-76566985 Msa1215330:exon
GGTGCAGGAGGAAGTGATGA+TGG 0.499986 8_1:-76565605 Msa1215330:CDS
GTGATGAAGGCGAGGAAGAA+GGG 0.503531 8_1:-76565655 Msa1215330:CDS
CTTCCAAGAAGGTGCTCCAA+AGG 0.509009 8_1:+76565788 None:intergenic
CCTCCTGCACCGTTAGCCTC+AGG 0.511442 8_1:+76565617 None:intergenic
CCCAGCCACCAGCAAAGAAG+AGG 0.521663 8_1:-76565490 Msa1215330:CDS
TTCCAAGAAGGTGCTCCAAA+GGG 0.531221 8_1:+76565789 None:intergenic
GAGTGTCGACGGCGACAGCT+TGG 0.539506 8_1:+76566904 None:intergenic
GTGCAGGAGGAAGTGATGAT+GGG 0.542524 8_1:-76565604 Msa1215330:CDS
CTTGAGTTGAGTTGAGTCCA+GGG 0.545804 8_1:-76567066 Msa1215330:five_prime_UTR
GGCTCCAAAGTTGACTGCAA+TGG 0.554467 8_1:-76565904 Msa1215330:CDS
AGTTCCACAAAACCCTAAAA+CGG 0.558071 8_1:-76567096 Msa1215330:five_prime_UTR
TGACTCCTCTCTCTCACTCT+CGG 0.562392 8_1:-76566991 Msa1215330:five_prime_UTR
TTAGCCTCAGGATCATCCTC+GGG 0.566086 8_1:+76565629 None:intergenic
GCGACAGCTTGGAGAAGAGA+GGG 0.568266 8_1:+76566915 None:intergenic
CATTGCCGAGAGTGAGAGAG+AGG 0.573058 8_1:+76566986 None:intergenic
AAGCTGGCTTCCTTCCAAGA+AGG 0.576452 8_1:+76565777 None:intergenic
AAGAGAGGGGAGGTCGACGA+CGG 0.580788 8_1:+76566929 None:intergenic
AGATGAAGAGGACGAAGAGG+AGG 0.581319 8_1:-76565534 Msa1215330:CDS
GGCGACAGCTTGGAGAAGAG+AGG 0.582331 8_1:+76566914 None:intergenic
GATGAGGAAGATGAAGAAGA+TGG 0.583508 8_1:-76565560 Msa1215330:CDS
GTTAGCCTCAGGATCATCCT+CGG 0.587042 8_1:+76565628 None:intergenic
TGATGAAGGCGAGGAAGAAG+GGG 0.595555 8_1:-76565654 Msa1215330:CDS
TGATGACGACGATGGCGATG+AGG 0.600233 8_1:-76565576 Msa1215330:CDS
CCTGAGGCTAACGGTGCAGG+AGG 0.603799 8_1:-76565617 Msa1215330:CDS
CGAAGATGAAGAGGACGAAG+AGG 0.605829 8_1:-76565537 Msa1215330:CDS
AGAAGATGGCGAAGATGAAG+AGG 0.607145 8_1:-76565546 Msa1215330:CDS
TCCACAAAACCCTAAAACGG+TGG 0.610189 8_1:-76567093 Msa1215330:five_prime_UTR
GGCGGAAACAAGAGTGTCGA+CGG 0.610291 8_1:+76566893 None:intergenic
TGATTAGAGCGAGTAAACCC+TGG 0.612107 8_1:+76567049 None:intergenic
GTACAAGATGAGGATGATGA+TGG 0.612789 8_1:-76565698 Msa1215330:CDS
AAGTGATACAGAAGATGATG+AGG 0.617223 8_1:-76565741 Msa1215330:CDS
CGAGGAAGAAGGGGATCCCG+AGG 0.625175 8_1:-76565645 Msa1215330:CDS
GGAGAAGAGGAAGACTACTC+CGG 0.625811 8_1:-76565677 Msa1215330:CDS
GACTAATGCTGCCAAATCAG+TGG 0.634709 8_1:-76565925 Msa1215330:CDS
CTACTCCGGTGATGAAGGCG+AGG 0.636930 8_1:-76565663 Msa1215330:CDS
TGCAGGAGGAAGTGATGATG+GGG 0.639993 8_1:-76565603 Msa1215330:CDS
TACGAGAGCGAGAGATTGAG+CGG 0.643982 8_1:+76566872 None:intergenic
GAGGATGATCCTGAGGCTAA+CGG 0.649850 8_1:-76565626 Msa1215330:CDS
CGACGACGGAGAGAATTGGA+AGG 0.661376 8_1:+76566943 None:intergenic
TCATTGACATAACTTGACAC+AGG 0.664341 8_1:+76565370 None:intergenic
CGACAGCTTGGAGAAGAGAG+GGG 0.672671 8_1:+76566916 None:intergenic
GGATCCCGAGGATGATCCTG+AGG 0.672779 8_1:-76565633 Msa1215330:CDS
CAGCTTGGAGAAGAGAGGGG+AGG 0.680758 8_1:+76566919 None:intergenic
TGATGATGACGTACAAGATG+AGG 0.687848 8_1:-76565708 Msa1215330:CDS
GGGGAAGATGATGACGACGA+TGG 0.692455 8_1:-76565584 Msa1215330:CDS
GAGAGCGAGAGATTGAGCGG+CGG 0.738155 8_1:+76566875 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGCATAGTAAAATTAAAA+GGG + chr8_1:76565504-76565523 None:intergenic 15.0%
!! AAATGCATAGTAAAATTAAA+AGG + chr8_1:76565505-76565524 None:intergenic 15.0%
!!! ATTTAGGATAAATAAACAAT+AGG - chr8_1:76565836-76565855 Msa1215330:intron 15.0%
!! AATGCATAGTAAAATTAAAA+GGG + chr8_1:76565504-76565523 None:intergenic 15.0%
!! AAATGCATAGTAAAATTAAA+AGG + chr8_1:76565505-76565524 None:intergenic 15.0%
!!! ATTTAGGATAAATAAACAAT+AGG - chr8_1:76565836-76565855 Msa1215330:intron 15.0%
!!! ATTAAAAGGGTGTAATTAAA+GGG + chr8_1:76565491-76565510 None:intergenic 20.0%
!!! AATTAAAAGGGTGTAATTAA+AGG + chr8_1:76565492-76565511 None:intergenic 20.0%
!!! AAAAAAAAAGTGCTAACAAA+TGG + chr8_1:76565624-76565643 None:intergenic 20.0%
!!! TAATTAAACTAAAGTATCCT+TGG - chr8_1:76566285-76566304 Msa1215330:intron 20.0%
!! TTATTCTGTTGTCTTTAATT+TGG - chr8_1:76566312-76566331 Msa1215330:intron 20.0%
!! GAATTATATTAGTAGGAAAT+TGG - chr8_1:76566852-76566871 Msa1215330:intron 20.0%
!!! AGTGTCATTTGTTTTTATTT+TGG - chr8_1:76567037-76567056 Msa1215330:five_prime_UTR 20.0%
!!! ATTAAAAGGGTGTAATTAAA+GGG + chr8_1:76565491-76565510 None:intergenic 20.0%
!!! AATTAAAAGGGTGTAATTAA+AGG + chr8_1:76565492-76565511 None:intergenic 20.0%
!!! AAAAAAAAAGTGCTAACAAA+TGG + chr8_1:76565624-76565643 None:intergenic 20.0%
!!! TAATTAAACTAAAGTATCCT+TGG - chr8_1:76566285-76566304 Msa1215330:intron 20.0%
!! TTATTCTGTTGTCTTTAATT+TGG - chr8_1:76566312-76566331 Msa1215330:intron 20.0%
!! GAATTATATTAGTAGGAAAT+TGG - chr8_1:76566852-76566871 Msa1215330:intron 20.0%
!!! AGTGTCATTTGTTTTTATTT+TGG - chr8_1:76567037-76567056 Msa1215330:five_prime_UTR 20.0%
!! TTAAAAGGGTGTAATTAAAG+GGG + chr8_1:76565490-76565509 None:intergenic 25.0%
!!! GTTTCTTTTTATTTCTACCA+CGG - chr8_1:76565785-76565804 Msa1215330:CDS 25.0%
!!! AGGCTAAATATTTTGGATTT+AGG - chr8_1:76565820-76565839 Msa1215330:intron 25.0%
! GAAGTGAGAATTATATTAGT+AGG - chr8_1:76566845-76566864 Msa1215330:intron 25.0%
! ATAAAAACAAATGACACTCA+CGG + chr8_1:76567035-76567054 None:intergenic 25.0%
!! TTAAAAGGGTGTAATTAAAG+GGG + chr8_1:76565490-76565509 None:intergenic 25.0%
!!! GTTTCTTTTTATTTCTACCA+CGG - chr8_1:76565785-76565804 Msa1215330:CDS 25.0%
!!! AGGCTAAATATTTTGGATTT+AGG - chr8_1:76565820-76565839 Msa1215330:intron 25.0%
! GAAGTGAGAATTATATTAGT+AGG - chr8_1:76566845-76566864 Msa1215330:intron 25.0%
! ATAAAAACAAATGACACTCA+CGG + chr8_1:76567035-76567054 None:intergenic 25.0%
TAAGCATGTTGTGGAAATTA+GGG - chr8_1:76565541-76565560 Msa1215330:CDS 30.0%
GAAGAATCATCACAGTTAAT+TGG + chr8_1:76565648-76565667 None:intergenic 30.0%
! CTGGTTTAGGCTAAATATTT+TGG - chr8_1:76565813-76565832 Msa1215330:intron 30.0%
AATATACAAGCATGCACATA+GGG + chr8_1:76565882-76565901 None:intergenic 30.0%
ACAATACATTAGTGCATAGT+TGG - chr8_1:76565967-76565986 Msa1215330:intron 30.0%
TATCCAAGCATATACTAAGA+AGG - chr8_1:76566075-76566094 Msa1215330:intron 30.0%
!! CTTTTCAAGACACAGAAAAA+CGG + chr8_1:76566165-76566184 None:intergenic 30.0%
GACAACAGAATAAAATACCA+AGG + chr8_1:76566305-76566324 None:intergenic 30.0%
!!! CTTTTGTTTTCTTTGTAAGC+TGG + chr8_1:76566555-76566574 None:intergenic 30.0%
TTAGTAGGAAATTGGTTATG+TGG - chr8_1:76566860-76566879 Msa1215330:intron 30.0%
TAAGCATGTTGTGGAAATTA+GGG - chr8_1:76565541-76565560 Msa1215330:CDS 30.0%
GAAGAATCATCACAGTTAAT+TGG + chr8_1:76565648-76565667 None:intergenic 30.0%
! CTGGTTTAGGCTAAATATTT+TGG - chr8_1:76565813-76565832 Msa1215330:intron 30.0%
AATATACAAGCATGCACATA+GGG + chr8_1:76565882-76565901 None:intergenic 30.0%
ACAATACATTAGTGCATAGT+TGG - chr8_1:76565967-76565986 Msa1215330:intron 30.0%
TATCCAAGCATATACTAAGA+AGG - chr8_1:76566075-76566094 Msa1215330:intron 30.0%
!! CTTTTCAAGACACAGAAAAA+CGG + chr8_1:76566165-76566184 None:intergenic 30.0%
GACAACAGAATAAAATACCA+AGG + chr8_1:76566305-76566324 None:intergenic 30.0%
!!! CTTTTGTTTTCTTTGTAAGC+TGG + chr8_1:76566555-76566574 None:intergenic 30.0%
TTAGTAGGAAATTGGTTATG+TGG - chr8_1:76566860-76566879 Msa1215330:intron 30.0%
AGTTCCACAAAACCCTAAAA+CGG - chr8_1:76565217-76565236 Msa1215330:three_prime_UTR 35.0%
GTCAACAACAACAATAGGTT+TGG + chr8_1:76565306-76565325 None:intergenic 35.0%
AACATGCTTACAGAGTTAAC+TGG + chr8_1:76565531-76565550 None:intergenic 35.0%
TTAACTCTGTAAGCATGTTG+TGG - chr8_1:76565532-76565551 Msa1215330:CDS 35.0%
GTAAGCATGTTGTGGAAATT+AGG - chr8_1:76565540-76565559 Msa1215330:CDS 35.0%
TTCAGTGGTATCATCTAACA+AGG + chr8_1:76565596-76565615 None:intergenic 35.0%
TGTTAGATGATACCACTGAA+TGG - chr8_1:76565596-76565615 Msa1215330:CDS 35.0%
TAACAAATGGAACCATTCAG+TGG + chr8_1:76565611-76565630 None:intergenic 35.0%
AGCACAGATACAACAATCAA+GGG + chr8_1:76565731-76565750 None:intergenic 35.0%
!!! CTTTTTATTTCTACCACGGT+TGG - chr8_1:76565789-76565808 Msa1215330:CDS 35.0%
CAATATACAAGCATGCACAT+AGG + chr8_1:76565883-76565902 None:intergenic 35.0%
GCATGCTTGTATATTGTTTG+TGG - chr8_1:76565887-76565906 Msa1215330:intron 35.0%
TTAGTGCATAGTTGGATTTG+CGG - chr8_1:76565975-76565994 Msa1215330:intron 35.0%
CTTCCTTCTTAGTATATGCT+TGG + chr8_1:76566081-76566100 None:intergenic 35.0%
! CTAGCGATCTTTTATAGCAT+AGG + chr8_1:76566132-76566151 None:intergenic 35.0%
TGTGTTCATCTATATGTCAC+AGG - chr8_1:76566232-76566251 Msa1215330:intron 35.0%
CTATATGTCACAGGTTTAGT+TGG - chr8_1:76566241-76566260 Msa1215330:intron 35.0%
!! AACATATGCGACTGTTTTGA+AGG - chr8_1:76566452-76566471 Msa1215330:intron 35.0%
ATCCTTGTTAAATCCTTGTC+AGG - chr8_1:76566499-76566518 Msa1215330:intron 35.0%
AACCTGACAAGGATTTAACA+AGG + chr8_1:76566504-76566523 None:intergenic 35.0%
AAGTGATACAGAAGATGATG+AGG - chr8_1:76566572-76566591 Msa1215330:intron 35.0%
TTGTGTCAAGCTTAGTGATT+AGG - chr8_1:76566890-76566909 Msa1215330:CDS 35.0%
TCATTGACATAACTTGACAC+AGG + chr8_1:76566946-76566965 None:intergenic 35.0%
AGTTCCACAAAACCCTAAAA+CGG - chr8_1:76565217-76565236 Msa1215330:three_prime_UTR 35.0%
GTCAACAACAACAATAGGTT+TGG + chr8_1:76565306-76565325 None:intergenic 35.0%
AACATGCTTACAGAGTTAAC+TGG + chr8_1:76565531-76565550 None:intergenic 35.0%
TTAACTCTGTAAGCATGTTG+TGG - chr8_1:76565532-76565551 Msa1215330:CDS 35.0%
GTAAGCATGTTGTGGAAATT+AGG - chr8_1:76565540-76565559 Msa1215330:CDS 35.0%
TTCAGTGGTATCATCTAACA+AGG + chr8_1:76565596-76565615 None:intergenic 35.0%
TGTTAGATGATACCACTGAA+TGG - chr8_1:76565596-76565615 Msa1215330:CDS 35.0%
TAACAAATGGAACCATTCAG+TGG + chr8_1:76565611-76565630 None:intergenic 35.0%
AGCACAGATACAACAATCAA+GGG + chr8_1:76565731-76565750 None:intergenic 35.0%
!!! CTTTTTATTTCTACCACGGT+TGG - chr8_1:76565789-76565808 Msa1215330:CDS 35.0%
CAATATACAAGCATGCACAT+AGG + chr8_1:76565883-76565902 None:intergenic 35.0%
GCATGCTTGTATATTGTTTG+TGG - chr8_1:76565887-76565906 Msa1215330:intron 35.0%
TTAGTGCATAGTTGGATTTG+CGG - chr8_1:76565975-76565994 Msa1215330:intron 35.0%
CTTCCTTCTTAGTATATGCT+TGG + chr8_1:76566081-76566100 None:intergenic 35.0%
! CTAGCGATCTTTTATAGCAT+AGG + chr8_1:76566132-76566151 None:intergenic 35.0%
TGTGTTCATCTATATGTCAC+AGG - chr8_1:76566232-76566251 Msa1215330:intron 35.0%
CTATATGTCACAGGTTTAGT+TGG - chr8_1:76566241-76566260 Msa1215330:intron 35.0%
!! AACATATGCGACTGTTTTGA+AGG - chr8_1:76566452-76566471 Msa1215330:intron 35.0%
ATCCTTGTTAAATCCTTGTC+AGG - chr8_1:76566499-76566518 Msa1215330:intron 35.0%
AACCTGACAAGGATTTAACA+AGG + chr8_1:76566504-76566523 None:intergenic 35.0%
AAGTGATACAGAAGATGATG+AGG - chr8_1:76566572-76566591 Msa1215330:intron 35.0%
TTGTGTCAAGCTTAGTGATT+AGG - chr8_1:76566890-76566909 Msa1215330:CDS 35.0%
TCATTGACATAACTTGACAC+AGG + chr8_1:76566946-76566965 None:intergenic 35.0%
! TCAAGTACTCCACCGTTTTA+GGG + chr8_1:76565232-76565251 None:intergenic 40.0%
GAGGAGTCAACAACAACAAT+AGG + chr8_1:76565311-76565330 None:intergenic 40.0%
GAGAATTGGAAGGTGAGATT+GGG + chr8_1:76565363-76565382 None:intergenic 40.0%
AGAGAATTGGAAGGTGAGAT+TGG + chr8_1:76565364-76565383 None:intergenic 40.0%
CAGCACAGATACAACAATCA+AGG + chr8_1:76565732-76565751 None:intergenic 40.0%
AGACACAGAAAAACGGACAA+GGG + chr8_1:76566158-76566177 None:intergenic 40.0%
AAGACACAGAAAAACGGACA+AGG + chr8_1:76566159-76566178 None:intergenic 40.0%
!! GTTTGTTTTTCTCCCCAGAT+TGG - chr8_1:76566356-76566375 Msa1215330:intron 40.0%
AGTCAGCAGATATCCAATCT+GGG + chr8_1:76566372-76566391 None:intergenic 40.0%
TAGTCAGCAGATATCCAATC+TGG + chr8_1:76566373-76566392 None:intergenic 40.0%
GAATCCATTGCAGTCAACTT+TGG + chr8_1:76566416-76566435 None:intergenic 40.0%
! TGATGATGACGTACAAGATG+AGG - chr8_1:76566605-76566624 Msa1215330:intron 40.0%
GTACAAGATGAGGATGATGA+TGG - chr8_1:76566615-76566634 Msa1215330:intron 40.0%
GATGAGGAAGATGAAGAAGA+TGG - chr8_1:76566753-76566772 Msa1215330:intron 40.0%
TGACACAGGTTCACTTACTA+AGG + chr8_1:76566932-76566951 None:intergenic 40.0%
CACTCACGGACAATAATAAC+TGG + chr8_1:76567021-76567040 None:intergenic 40.0%
ATTGCTCGTGCTATCAATCT+TGG - chr8_1:76567072-76567091 Msa1215330:five_prime_UTR 40.0%
! TCAAGTACTCCACCGTTTTA+GGG + chr8_1:76565232-76565251 None:intergenic 40.0%
GAGGAGTCAACAACAACAAT+AGG + chr8_1:76565311-76565330 None:intergenic 40.0%
GAGAATTGGAAGGTGAGATT+GGG + chr8_1:76565363-76565382 None:intergenic 40.0%
AGAGAATTGGAAGGTGAGAT+TGG + chr8_1:76565364-76565383 None:intergenic 40.0%
CAGCACAGATACAACAATCA+AGG + chr8_1:76565732-76565751 None:intergenic 40.0%
AGACACAGAAAAACGGACAA+GGG + chr8_1:76566158-76566177 None:intergenic 40.0%
AAGACACAGAAAAACGGACA+AGG + chr8_1:76566159-76566178 None:intergenic 40.0%
!! GTTTGTTTTTCTCCCCAGAT+TGG - chr8_1:76566356-76566375 Msa1215330:intron 40.0%
AGTCAGCAGATATCCAATCT+GGG + chr8_1:76566372-76566391 None:intergenic 40.0%
TAGTCAGCAGATATCCAATC+TGG + chr8_1:76566373-76566392 None:intergenic 40.0%
GAATCCATTGCAGTCAACTT+TGG + chr8_1:76566416-76566435 None:intergenic 40.0%
! TGATGATGACGTACAAGATG+AGG - chr8_1:76566605-76566624 Msa1215330:intron 40.0%
GTACAAGATGAGGATGATGA+TGG - chr8_1:76566615-76566634 Msa1215330:intron 40.0%
GATGAGGAAGATGAAGAAGA+TGG - chr8_1:76566753-76566772 Msa1215330:intron 40.0%
TGACACAGGTTCACTTACTA+AGG + chr8_1:76566932-76566951 None:intergenic 40.0%
CACTCACGGACAATAATAAC+TGG + chr8_1:76567021-76567040 None:intergenic 40.0%
ATTGCTCGTGCTATCAATCT+TGG - chr8_1:76567072-76567091 Msa1215330:five_prime_UTR 40.0%
TCCACAAAACCCTAAAACGG+TGG - chr8_1:76565220-76565239 Msa1215330:three_prime_UTR 45.0%
!!! TCCACCGTTTTAGGGTTTTG+TGG + chr8_1:76565224-76565243 None:intergenic 45.0%
! CTCAAGTACTCCACCGTTTT+AGG + chr8_1:76565233-76565252 None:intergenic 45.0%
!! ACTTGAGTTGAGTTGAGTCC+AGG - chr8_1:76565246-76565265 Msa1215330:three_prime_UTR 45.0%
!! CTTGAGTTGAGTTGAGTCCA+GGG - chr8_1:76565247-76565266 Msa1215330:three_prime_UTR 45.0%
TGATTAGAGCGAGTAAACCC+TGG + chr8_1:76565267-76565286 None:intergenic 45.0%
CTAACAAGGCACAGAGTTCA+AGG + chr8_1:76565582-76565601 None:intergenic 45.0%
! TATTTCTACCACGGTTGGAC+TGG - chr8_1:76565794-76565813 Msa1215330:CDS 45.0%
AGTTGGATTTGCGGTGAGAT+TGG - chr8_1:76565984-76566003 Msa1215330:intron 45.0%
GTCAGCAGATATCCAATCTG+GGG + chr8_1:76566371-76566390 None:intergenic 45.0%
GACTAATGCTGCCAAATCAG+TGG - chr8_1:76566388-76566407 Msa1215330:intron 45.0%
!! CAACTTTGGAGCCACTGATT+TGG + chr8_1:76566402-76566421 None:intergenic 45.0%
AATCCTTGTCAGGTTCCCTT+TGG - chr8_1:76566509-76566528 Msa1215330:intron 45.0%
!! TTCCAAGAAGGTGCTCCAAA+GGG + chr8_1:76566527-76566546 None:intergenic 45.0%
!! TGAGGATGATGATGGAGAAG+AGG - chr8_1:76566623-76566642 Msa1215330:intron 45.0%
AGAAGATGGCGAAGATGAAG+AGG - chr8_1:76566767-76566786 Msa1215330:intron 45.0%
TCTCACTTCCTCTTCTTTGC+TGG + chr8_1:76566834-76566853 None:intergenic 45.0%
TCCACAAAACCCTAAAACGG+TGG - chr8_1:76565220-76565239 Msa1215330:three_prime_UTR 45.0%
!!! TCCACCGTTTTAGGGTTTTG+TGG + chr8_1:76565224-76565243 None:intergenic 45.0%
! CTCAAGTACTCCACCGTTTT+AGG + chr8_1:76565233-76565252 None:intergenic 45.0%
!! ACTTGAGTTGAGTTGAGTCC+AGG - chr8_1:76565246-76565265 Msa1215330:three_prime_UTR 45.0%
!! CTTGAGTTGAGTTGAGTCCA+GGG - chr8_1:76565247-76565266 Msa1215330:three_prime_UTR 45.0%
TGATTAGAGCGAGTAAACCC+TGG + chr8_1:76565267-76565286 None:intergenic 45.0%
CTAACAAGGCACAGAGTTCA+AGG + chr8_1:76565582-76565601 None:intergenic 45.0%
! TATTTCTACCACGGTTGGAC+TGG - chr8_1:76565794-76565813 Msa1215330:CDS 45.0%
AGTTGGATTTGCGGTGAGAT+TGG - chr8_1:76565984-76566003 Msa1215330:intron 45.0%
GTCAGCAGATATCCAATCTG+GGG + chr8_1:76566371-76566390 None:intergenic 45.0%
GACTAATGCTGCCAAATCAG+TGG - chr8_1:76566388-76566407 Msa1215330:intron 45.0%
!! CAACTTTGGAGCCACTGATT+TGG + chr8_1:76566402-76566421 None:intergenic 45.0%
AATCCTTGTCAGGTTCCCTT+TGG - chr8_1:76566509-76566528 Msa1215330:intron 45.0%
!! TTCCAAGAAGGTGCTCCAAA+GGG + chr8_1:76566527-76566546 None:intergenic 45.0%
!! TGAGGATGATGATGGAGAAG+AGG - chr8_1:76566623-76566642 Msa1215330:intron 45.0%
AGAAGATGGCGAAGATGAAG+AGG - chr8_1:76566767-76566786 Msa1215330:intron 45.0%
TCTCACTTCCTCTTCTTTGC+TGG + chr8_1:76566834-76566853 None:intergenic 45.0%
!! AAAAAAAAAAAAAAAAAAAC+AGG + chr8_1:76567098-76567117 None:intergenic 5.0%
!! AAAAAAAAAAAAAAAAAAAC+AGG + chr8_1:76567098-76567117 None:intergenic 5.0%
TGACTCCTCTCTCTCACTCT+CGG - chr8_1:76565322-76565341 Msa1215330:three_prime_UTR 50.0%
TACGAGAGCGAGAGATTGAG+CGG + chr8_1:76565444-76565463 None:intergenic 50.0%
!! TACCACGGTTGGACTGGTTT+AGG - chr8_1:76565800-76565819 Msa1215330:intron 50.0%
GGCTCCAAAGTTGACTGCAA+TGG - chr8_1:76566409-76566428 Msa1215330:intron 50.0%
!! TTCCCTTTGGAGCACCTTCT+TGG - chr8_1:76566522-76566541 Msa1215330:intron 50.0%
!! CTTCCAAGAAGGTGCTCCAA+AGG + chr8_1:76566528-76566547 None:intergenic 50.0%
!! CTTTGGAGCACCTTCTTGGA+AGG - chr8_1:76566526-76566545 Msa1215330:intron 50.0%
! AAGCTGGCTTCCTTCCAAGA+AGG + chr8_1:76566539-76566558 None:intergenic 50.0%
GGAGAAGAGGAAGACTACTC+CGG - chr8_1:76566636-76566655 Msa1215330:intron 50.0%
GAAGACTACTCCGGTGATGA+AGG - chr8_1:76566645-76566664 Msa1215330:intron 50.0%
TTCTTCCTCGCCTTCATCAC+CGG + chr8_1:76566658-76566677 None:intergenic 50.0%
GTGATGAAGGCGAGGAAGAA+GGG - chr8_1:76566658-76566677 Msa1215330:intron 50.0%
TGATGAAGGCGAGGAAGAAG+GGG - chr8_1:76566659-76566678 Msa1215330:intron 50.0%
TTAGCCTCAGGATCATCCTC+GGG + chr8_1:76566687-76566706 None:intergenic 50.0%
! GTTAGCCTCAGGATCATCCT+CGG + chr8_1:76566688-76566707 None:intergenic 50.0%
GAGGATGATCCTGAGGCTAA+CGG - chr8_1:76566687-76566706 Msa1215330:intron 50.0%
GTGCAGGAGGAAGTGATGAT+GGG - chr8_1:76566709-76566728 Msa1215330:intron 50.0%
! TGCAGGAGGAAGTGATGATG+GGG - chr8_1:76566710-76566729 Msa1215330:intron 50.0%
CGAAGATGAAGAGGACGAAG+AGG - chr8_1:76566776-76566795 Msa1215330:intron 50.0%
AGATGAAGAGGACGAAGAGG+AGG - chr8_1:76566779-76566798 Msa1215330:intron 50.0%
CACTTCCTCTTCTTTGCTGG+TGG + chr8_1:76566831-76566850 None:intergenic 50.0%
TGACTCCTCTCTCTCACTCT+CGG - chr8_1:76565322-76565341 Msa1215330:three_prime_UTR 50.0%
TACGAGAGCGAGAGATTGAG+CGG + chr8_1:76565444-76565463 None:intergenic 50.0%
!! TACCACGGTTGGACTGGTTT+AGG - chr8_1:76565800-76565819 Msa1215330:intron 50.0%
GGCTCCAAAGTTGACTGCAA+TGG - chr8_1:76566409-76566428 Msa1215330:intron 50.0%
!! TTCCCTTTGGAGCACCTTCT+TGG - chr8_1:76566522-76566541 Msa1215330:intron 50.0%
!! CTTCCAAGAAGGTGCTCCAA+AGG + chr8_1:76566528-76566547 None:intergenic 50.0%
!! CTTTGGAGCACCTTCTTGGA+AGG - chr8_1:76566526-76566545 Msa1215330:intron 50.0%
! AAGCTGGCTTCCTTCCAAGA+AGG + chr8_1:76566539-76566558 None:intergenic 50.0%
GGAGAAGAGGAAGACTACTC+CGG - chr8_1:76566636-76566655 Msa1215330:intron 50.0%
GAAGACTACTCCGGTGATGA+AGG - chr8_1:76566645-76566664 Msa1215330:intron 50.0%
TTCTTCCTCGCCTTCATCAC+CGG + chr8_1:76566658-76566677 None:intergenic 50.0%
GTGATGAAGGCGAGGAAGAA+GGG - chr8_1:76566658-76566677 Msa1215330:intron 50.0%
TGATGAAGGCGAGGAAGAAG+GGG - chr8_1:76566659-76566678 Msa1215330:intron 50.0%
TTAGCCTCAGGATCATCCTC+GGG + chr8_1:76566687-76566706 None:intergenic 50.0%
! GTTAGCCTCAGGATCATCCT+CGG + chr8_1:76566688-76566707 None:intergenic 50.0%
GAGGATGATCCTGAGGCTAA+CGG - chr8_1:76566687-76566706 Msa1215330:intron 50.0%
GTGCAGGAGGAAGTGATGAT+GGG - chr8_1:76566709-76566728 Msa1215330:intron 50.0%
! TGCAGGAGGAAGTGATGATG+GGG - chr8_1:76566710-76566729 Msa1215330:intron 50.0%
CGAAGATGAAGAGGACGAAG+AGG - chr8_1:76566776-76566795 Msa1215330:intron 50.0%
AGATGAAGAGGACGAAGAGG+AGG - chr8_1:76566779-76566798 Msa1215330:intron 50.0%
CACTTCCTCTTCTTTGCTGG+TGG + chr8_1:76566831-76566850 None:intergenic 50.0%
CATTGCCGAGAGTGAGAGAG+AGG + chr8_1:76565330-76565349 None:intergenic 55.0%
CTCTCTCTCACTCTCGGCAA+TGG - chr8_1:76565328-76565347 Msa1215330:three_prime_UTR 55.0%
CGACGACGGAGAGAATTGGA+AGG + chr8_1:76565373-76565392 None:intergenic 55.0%
AGGTCGACGACGGAGAGAAT+TGG + chr8_1:76565377-76565396 None:intergenic 55.0%
CGACAGCTTGGAGAAGAGAG+GGG + chr8_1:76565400-76565419 None:intergenic 55.0%
GCGACAGCTTGGAGAAGAGA+GGG + chr8_1:76565401-76565420 None:intergenic 55.0%
!! GGCGGAAACAAGAGTGTCGA+CGG + chr8_1:76565423-76565442 None:intergenic 55.0%
AGCCTAAACCAGTCCAACCG+TGG + chr8_1:76565805-76565824 None:intergenic 55.0%
GCTCCAAAGGGAACCTGACA+AGG + chr8_1:76566515-76566534 None:intergenic 55.0%
GGTGATGAAGGCGAGGAAGA+AGG - chr8_1:76566657-76566676 Msa1215330:intron 55.0%
GGTGCAGGAGGAAGTGATGA+TGG - chr8_1:76566708-76566727 Msa1215330:intron 55.0%
! GGGGAAGATGATGACGACGA+TGG - chr8_1:76566729-76566748 Msa1215330:intron 55.0%
! TGATGACGACGATGGCGATG+AGG - chr8_1:76566737-76566756 Msa1215330:intron 55.0%
AGAGGACGAAGAGGAGGAAG+AGG - chr8_1:76566785-76566804 Msa1215330:intron 55.0%
CTCTTCTTTGCTGGTGGCTG+GGG + chr8_1:76566825-76566844 None:intergenic 55.0%
CCTCTTCTTTGCTGGTGGCT+GGG + chr8_1:76566826-76566845 None:intergenic 55.0%
TCCTCTTCTTTGCTGGTGGC+TGG + chr8_1:76566827-76566846 None:intergenic 55.0%
CATTGCCGAGAGTGAGAGAG+AGG + chr8_1:76565330-76565349 None:intergenic 55.0%
CTCTCTCTCACTCTCGGCAA+TGG - chr8_1:76565328-76565347 Msa1215330:three_prime_UTR 55.0%
CGACGACGGAGAGAATTGGA+AGG + chr8_1:76565373-76565392 None:intergenic 55.0%
AGGTCGACGACGGAGAGAAT+TGG + chr8_1:76565377-76565396 None:intergenic 55.0%
CGACAGCTTGGAGAAGAGAG+GGG + chr8_1:76565400-76565419 None:intergenic 55.0%
GCGACAGCTTGGAGAAGAGA+GGG + chr8_1:76565401-76565420 None:intergenic 55.0%
!! GGCGGAAACAAGAGTGTCGA+CGG + chr8_1:76565423-76565442 None:intergenic 55.0%
AGCCTAAACCAGTCCAACCG+TGG + chr8_1:76565805-76565824 None:intergenic 55.0%
GCTCCAAAGGGAACCTGACA+AGG + chr8_1:76566515-76566534 None:intergenic 55.0%
GGTGATGAAGGCGAGGAAGA+AGG - chr8_1:76566657-76566676 Msa1215330:intron 55.0%
GGTGCAGGAGGAAGTGATGA+TGG - chr8_1:76566708-76566727 Msa1215330:intron 55.0%
! GGGGAAGATGATGACGACGA+TGG - chr8_1:76566729-76566748 Msa1215330:intron 55.0%
! TGATGACGACGATGGCGATG+AGG - chr8_1:76566737-76566756 Msa1215330:intron 55.0%
AGAGGACGAAGAGGAGGAAG+AGG - chr8_1:76566785-76566804 Msa1215330:intron 55.0%
CTCTTCTTTGCTGGTGGCTG+GGG + chr8_1:76566825-76566844 None:intergenic 55.0%
CCTCTTCTTTGCTGGTGGCT+GGG + chr8_1:76566826-76566845 None:intergenic 55.0%
TCCTCTTCTTTGCTGGTGGC+TGG + chr8_1:76566827-76566846 None:intergenic 55.0%
AAGAGAGGGGAGGTCGACGA+CGG + chr8_1:76565387-76565406 None:intergenic 60.0%
CAGCTTGGAGAAGAGAGGGG+AGG + chr8_1:76565397-76565416 None:intergenic 60.0%
GGCGACAGCTTGGAGAAGAG+AGG + chr8_1:76565402-76565421 None:intergenic 60.0%
GAGAGCGAGAGATTGAGCGG+CGG + chr8_1:76565441-76565460 None:intergenic 60.0%
CTACTCCGGTGATGAAGGCG+AGG - chr8_1:76566650-76566669 Msa1215330:intron 60.0%
GGATCCCGAGGATGATCCTG+AGG - chr8_1:76566680-76566699 Msa1215330:intron 60.0%
GATCCTGAGGCTAACGGTGC+AGG - chr8_1:76566693-76566712 Msa1215330:intron 60.0%
CCCAGCCACCAGCAAAGAAG+AGG - chr8_1:76566823-76566842 Msa1215330:intron 60.0%
AAGAGAGGGGAGGTCGACGA+CGG + chr8_1:76565387-76565406 None:intergenic 60.0%
CAGCTTGGAGAAGAGAGGGG+AGG + chr8_1:76565397-76565416 None:intergenic 60.0%
GGCGACAGCTTGGAGAAGAG+AGG + chr8_1:76565402-76565421 None:intergenic 60.0%
GAGAGCGAGAGATTGAGCGG+CGG + chr8_1:76565441-76565460 None:intergenic 60.0%
CTACTCCGGTGATGAAGGCG+AGG - chr8_1:76566650-76566669 Msa1215330:intron 60.0%
GGATCCCGAGGATGATCCTG+AGG - chr8_1:76566680-76566699 Msa1215330:intron 60.0%
GATCCTGAGGCTAACGGTGC+AGG - chr8_1:76566693-76566712 Msa1215330:intron 60.0%
CCCAGCCACCAGCAAAGAAG+AGG - chr8_1:76566823-76566842 Msa1215330:intron 60.0%
!! GAGTGTCGACGGCGACAGCT+TGG + chr8_1:76565412-76565431 None:intergenic 65.0%
CGAGGAAGAAGGGGATCCCG+AGG - chr8_1:76566668-76566687 Msa1215330:intron 65.0%
! CCTCCTGCACCGTTAGCCTC+AGG + chr8_1:76566699-76566718 None:intergenic 65.0%
CCTGAGGCTAACGGTGCAGG+AGG - chr8_1:76566696-76566715 Msa1215330:intron 65.0%
!! GAGTGTCGACGGCGACAGCT+TGG + chr8_1:76565412-76565431 None:intergenic 65.0%
CGAGGAAGAAGGGGATCCCG+AGG - chr8_1:76566668-76566687 Msa1215330:intron 65.0%
! CCTCCTGCACCGTTAGCCTC+AGG + chr8_1:76566699-76566718 None:intergenic 65.0%
CCTGAGGCTAACGGTGCAGG+AGG - chr8_1:76566696-76566715 Msa1215330:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 76565214 76567121 76565214 ID=Msa1215330;Name=Msa1215330
chr8_1 mRNA 76565214 76567121 76565214 ID=Msa1215330-mRNA-1;Parent=Msa1215330;Name=Msa1215330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=133|1|1|1|1|1|3|270|169
chr8_1 exon 76565214 76565814 76565214 ID=Msa1215330-mRNA-1:exon:26281;Parent=Msa1215330-mRNA-1
chr8_1 exon 76565897 76565961 76565897 ID=Msa1215330-mRNA-1:exon:26280;Parent=Msa1215330-mRNA-1
chr8_1 exon 76566875 76567121 76566875 ID=Msa1215330-mRNA-1:exon:26279;Parent=Msa1215330-mRNA-1
chr8_1 five_prime_UTR 76566989 76567121 76566989 ID=Msa1215330-mRNA-1:five_prime_utr;Parent=Msa1215330-mRNA-1
chr8_1 CDS 76566875 76566988 76566875 ID=Msa1215330-mRNA-1:cds;Parent=Msa1215330-mRNA-1
chr8_1 CDS 76565897 76565961 76565897 ID=Msa1215330-mRNA-1:cds;Parent=Msa1215330-mRNA-1
chr8_1 CDS 76565484 76565814 76565484 ID=Msa1215330-mRNA-1:cds;Parent=Msa1215330-mRNA-1
chr8_1 three_prime_UTR 76565214 76565483 76565214 ID=Msa1215330-mRNA-1:three_prime_utr;Parent=Msa1215330-mRNA-1
Gene Sequence

>Msa1215330

ATGGCTGTGTCAACCCAATCTCACCTTCCAATTCTCTCCGTCGTCGACCTCCCCTCTCTTCTCCAAGCTGTCGCCGTCGACACTCTTGTTTCCGCCGCTCAATCTCTCGCTCTCATTGGATATCTGCTGACTAATGCTGCCAAATCAGTGGCTCCAAAGTTGACTGCAATGGATTCAATGTTCCCTTTGGAGCACCTTCTTGGAAGGAAGCCAGCTTACAAAGAAAACAAAAGTGATACAGAAGATGATGAGGACGACGACGATGATGATGACGTACAAGATGAGGATGATGATGGAGAAGAGGAAGACTACTCCGGTGATGAAGGCGAGGAAGAAGGGGATCCCGAGGATGATCCTGAGGCTAACGGTGCAGGAGGAAGTGATGATGGGGAAGATGATGACGACGATGGCGATGAGGAAGATGAAGAAGATGGCGAAGATGAAGAGGACGAAGAGGAGGAAGAGGAAGATGATGAGACTCCCCAGCCACCAGCAAAGAAGAGGAAGTGA
Protein sequence

>Msa1215330

MAVSTQSHLPILSVVDLPSLLQAVAVDTLVSAAQSLALIGYLLTNAAKSVAPKLTAMDSMFPLEHLLGRKPAYKENKSDTEDDEDDDDDDDVQDEDDDGEEEDYSGDEGEEEGDPEDDPEANGAGGSDDGEDDDDDGDEEDEEDGEDEEDEEEEEEDDETPQPPAKKRK*