AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1404870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1404870 MtrunA17_Chr4g0064691 97.041 169 5 0 1 169 1 169 3.01e-109 308
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCTTCTTTGCTGGTGGC+TGG 0.201684 tig0023091:+80145 None:intergenic
TCTCACTTCCTCTTCTTTGC+TGG 0.250916 tig0023091:+80138 None:intergenic
TTCCCTTTGGAGCACCTTCT+TGG 0.276978 tig0023091:-80447 Msa1404870:CDS
CCTCTTCTTTGCTGGTGGCT+GGG 0.297534 tig0023091:+80146 None:intergenic
TTGTGTCAAGCTTAGTGATT+AGG 0.324884 tig0023091:-80079 Msa1404870:three_prime_UTR
CTCTTCTTTGCTGGTGGCTG+GGG 0.325642 tig0023091:+80147 None:intergenic
GAATTATATTAGTAGGAAAT+TGG 0.326206 tig0023091:-80117 Msa1404870:three_prime_UTR
CAACTTTGGAGCCACTGATT+TGG 0.351405 tig0023091:+80570 None:intergenic
ATTGCTTGTGCTATCAATCT+TGG 0.352766 tig0023091:-79897 Msa1404870:three_prime_UTR
AGAGAATTGGAAGGTGAGAT+TGG 0.354776 tig0023091:+81608 None:intergenic
CACTCACGGACAATAATAAC+TGG 0.374970 tig0023091:+79951 None:intergenic
AATCCTTGTCAGGTTCCCTT+TGG 0.384520 tig0023091:-80460 Msa1404870:intron
TGACACAGGTTCACTTACTA+AGG 0.409062 tig0023091:+80040 None:intergenic
ACTTGAGTTGAGTTGAGTCC+AGG 0.410529 tig0023091:-81723 Msa1404870:five_prime_UTR
GAATCCATTGCAGTCAACTT+TGG 0.411321 tig0023091:+80556 None:intergenic
CACTTCCTCTTCTTTGCTGG+TGG 0.417470 tig0023091:+80141 None:intergenic
TTCTTCCTCGCCTTCATCAC+CGG 0.419016 tig0023091:+80314 None:intergenic
GAGGAGTCAACAACAACAAT+AGG 0.424929 tig0023091:+81661 None:intergenic
AGAGGACGAAGAGGAGGAAG+AGG 0.432417 tig0023091:-80184 Msa1404870:CDS
TTAGTAGGAAATTGGTTATG+TGG 0.455585 tig0023091:-80109 Msa1404870:three_prime_UTR
AGGTCGACGACGGAGAGAAT+TGG 0.458122 tig0023091:+81595 None:intergenic
TGAGGATGATGATGGAGAAG+AGG 0.459842 tig0023091:-80346 Msa1404870:CDS
GAAGTGAGAATTATATTAGT+AGG 0.468566 tig0023091:-80124 Msa1404870:exon
GATCCTGAGGCTAACGGTGC+AGG 0.476679 tig0023091:-80276 Msa1404870:CDS
GTCAACAACAACAATAGGTT+TGG 0.480887 tig0023091:+81666 None:intergenic
GGTGATGAAGGCGAGGAAGA+AGG 0.491443 tig0023091:-80312 Msa1404870:CDS
GAGAATTGGAAGGTGAGATT+GGG 0.491676 tig0023091:+81609 None:intergenic
GAAGACTACTCCGGTGATGA+AGG 0.492863 tig0023091:-80324 Msa1404870:CDS
CTTTGGAGCACCTTCTTGGA+AGG 0.496704 tig0023091:-80443 Msa1404870:CDS
CTCTCTCTCACTCTCGGCAA+TGG 0.497209 tig0023091:-81641 Msa1404870:exon
GGTGCAGGAGGAAGTGATGA+TGG 0.499986 tig0023091:-80261 Msa1404870:CDS
GTGATGAAGGCGAGGAAGAA+GGG 0.503531 tig0023091:-80311 Msa1404870:CDS
CTTCCAAGAAGGTGCTCCAA+AGG 0.509009 tig0023091:+80444 None:intergenic
CCTCCTGCACCGTTAGCCTC+AGG 0.511442 tig0023091:+80273 None:intergenic
CCCAGCCACCAGCAAAGAAG+AGG 0.521663 tig0023091:-80146 Msa1404870:CDS
TTCCAAGAAGGTGCTCCAAA+GGG 0.531221 tig0023091:+80445 None:intergenic
GAGTGTCGACGGCGACAGCT+TGG 0.539506 tig0023091:+81560 None:intergenic
GTGCAGGAGGAAGTGATGAT+GGG 0.542524 tig0023091:-80260 Msa1404870:CDS
CTTGAGTTGAGTTGAGTCCA+GGG 0.545804 tig0023091:-81722 Msa1404870:five_prime_UTR
GGCTCCAAAGTTGACTGCAA+TGG 0.554467 tig0023091:-80560 Msa1404870:CDS
AGTTCCACAAAACCCTAAAA+CGG 0.558071 tig0023091:-81752 Msa1404870:five_prime_UTR
TGACTCCTCTCTCTCACTCT+CGG 0.562392 tig0023091:-81647 Msa1404870:five_prime_UTR
TTAGCCTCAGGATCATCCTC+GGG 0.566086 tig0023091:+80285 None:intergenic
GCGACAGCTTGGAGAAGAGA+GGG 0.568266 tig0023091:+81571 None:intergenic
CATTGCCGAGAGTGAGAGAG+AGG 0.573058 tig0023091:+81642 None:intergenic
AAGCTGGCTTCCTTCCAAGA+AGG 0.576452 tig0023091:+80433 None:intergenic
AAGAGAGGGGAGGTCGACGA+CGG 0.580788 tig0023091:+81585 None:intergenic
AGATGAAGAGGACGAAGAGG+AGG 0.581319 tig0023091:-80190 Msa1404870:CDS
GGCGACAGCTTGGAGAAGAG+AGG 0.582331 tig0023091:+81570 None:intergenic
GATGAGGAAGATGAAGAAGA+TGG 0.583508 tig0023091:-80216 Msa1404870:CDS
GTTAGCCTCAGGATCATCCT+CGG 0.587042 tig0023091:+80284 None:intergenic
TGATGAAGGCGAGGAAGAAG+GGG 0.595555 tig0023091:-80310 Msa1404870:CDS
CCTGAGGCTAACGGTGCAGG+AGG 0.603799 tig0023091:-80273 Msa1404870:CDS
CGAAGATGAAGAGGACGAAG+AGG 0.605829 tig0023091:-80193 Msa1404870:CDS
AGAAGATGGCGAAGATGAAG+AGG 0.607145 tig0023091:-80202 Msa1404870:CDS
TCCACAAAACCCTAAAACGG+TGG 0.610189 tig0023091:-81749 Msa1404870:five_prime_UTR
GGCGGAAACAAGAGTGTCGA+CGG 0.610291 tig0023091:+81549 None:intergenic
TGATTAGAGCGAGTAAACCC+TGG 0.612107 tig0023091:+81705 None:intergenic
GTACAAGATGAGGATGATGA+TGG 0.612789 tig0023091:-80354 Msa1404870:CDS
AAGTGATACAGAAGATGATG+AGG 0.617223 tig0023091:-80397 Msa1404870:CDS
CGAGGAAGAAGGGGATCCCG+AGG 0.625175 tig0023091:-80301 Msa1404870:CDS
GGAGAAGAGGAAGACTACTC+CGG 0.625811 tig0023091:-80333 Msa1404870:CDS
GACTAATGCTGCCAAATCAG+TGG 0.634709 tig0023091:-80581 Msa1404870:CDS
CTACTCCGGTGATGAAGGCG+AGG 0.636930 tig0023091:-80319 Msa1404870:CDS
TGCAGGAGGAAGTGATGATG+GGG 0.639993 tig0023091:-80259 Msa1404870:CDS
TACGAGAGCGAGAGATTGAG+CGG 0.643982 tig0023091:+81528 None:intergenic
GAGGATGATCCTGAGGCTAA+CGG 0.649850 tig0023091:-80282 Msa1404870:CDS
CGACGACGGAGAGAATTGGA+AGG 0.661376 tig0023091:+81599 None:intergenic
TCATTGACATAACTTGACAC+AGG 0.664341 tig0023091:+80026 None:intergenic
CGACAGCTTGGAGAAGAGAG+GGG 0.672671 tig0023091:+81572 None:intergenic
GGATCCCGAGGATGATCCTG+AGG 0.672779 tig0023091:-80289 Msa1404870:CDS
CAGCTTGGAGAAGAGAGGGG+AGG 0.680758 tig0023091:+81575 None:intergenic
TGATGATGACGTACAAGATG+AGG 0.687848 tig0023091:-80364 Msa1404870:CDS
TGATGATGACGATGGCGATG+AGG 0.692718 tig0023091:-80232 Msa1404870:CDS
GGGGAAGATGATGATGACGA+TGG 0.701589 tig0023091:-80240 Msa1404870:CDS
GAGAGCGAGAGATTGAGCGG+CGG 0.738155 tig0023091:+81531 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGCATAGTAAAATTAAAA+GGG + tig0023091:80153-80172 None:intergenic 15.0%
!! AAATGCATAGTAAAATTAAA+AGG + tig0023091:80154-80173 None:intergenic 15.0%
!!! ATTTAGGATAAATAAACAAT+AGG - tig0023091:80485-80504 Msa1404870:intron 15.0%
!! AATGCATAGTAAAATTAAAA+GGG + tig0023091:80153-80172 None:intergenic 15.0%
!! AAATGCATAGTAAAATTAAA+AGG + tig0023091:80154-80173 None:intergenic 15.0%
!!! ATTTAGGATAAATAAACAAT+AGG - tig0023091:80485-80504 Msa1404870:intron 15.0%
!!! ATTAAAAGGGTGTAATTAAA+GGG + tig0023091:80140-80159 None:intergenic 20.0%
!!! AATTAAAAGGGTGTAATTAA+AGG + tig0023091:80141-80160 None:intergenic 20.0%
!!! AAAAAAAAAGTGCTAACAAA+TGG + tig0023091:80273-80292 None:intergenic 20.0%
!!! TAATTAAACTAAAGTATCCT+TGG - tig0023091:80934-80953 Msa1404870:intron 20.0%
!! TTATTCTGTTGTCTTTAATT+TGG - tig0023091:80961-80980 Msa1404870:intron 20.0%
!! GAATTATATTAGTAGGAAAT+TGG - tig0023091:81501-81520 Msa1404870:intron 20.0%
!!! AGTGTCATTTGTTTTTATTT+TGG - tig0023091:81686-81705 Msa1404870:five_prime_UTR 20.0%
!!! ATTAAAAGGGTGTAATTAAA+GGG + tig0023091:80140-80159 None:intergenic 20.0%
!!! AATTAAAAGGGTGTAATTAA+AGG + tig0023091:80141-80160 None:intergenic 20.0%
!!! AAAAAAAAAGTGCTAACAAA+TGG + tig0023091:80273-80292 None:intergenic 20.0%
!!! TAATTAAACTAAAGTATCCT+TGG - tig0023091:80934-80953 Msa1404870:intron 20.0%
!! TTATTCTGTTGTCTTTAATT+TGG - tig0023091:80961-80980 Msa1404870:intron 20.0%
!! GAATTATATTAGTAGGAAAT+TGG - tig0023091:81501-81520 Msa1404870:intron 20.0%
!!! AGTGTCATTTGTTTTTATTT+TGG - tig0023091:81686-81705 Msa1404870:five_prime_UTR 20.0%
!! TTAAAAGGGTGTAATTAAAG+GGG + tig0023091:80139-80158 None:intergenic 25.0%
!!! GTTTCTTTTTATTTCTACCA+CGG - tig0023091:80434-80453 Msa1404870:CDS 25.0%
!!! AGGCTAAATATTTTGGATTT+AGG - tig0023091:80469-80488 Msa1404870:intron 25.0%
! GAAGTGAGAATTATATTAGT+AGG - tig0023091:81494-81513 Msa1404870:intron 25.0%
! ATAAAAACAAATGACACTCA+CGG + tig0023091:81684-81703 None:intergenic 25.0%
!! TTAAAAGGGTGTAATTAAAG+GGG + tig0023091:80139-80158 None:intergenic 25.0%
!!! GTTTCTTTTTATTTCTACCA+CGG - tig0023091:80434-80453 Msa1404870:CDS 25.0%
!!! AGGCTAAATATTTTGGATTT+AGG - tig0023091:80469-80488 Msa1404870:intron 25.0%
! GAAGTGAGAATTATATTAGT+AGG - tig0023091:81494-81513 Msa1404870:intron 25.0%
! ATAAAAACAAATGACACTCA+CGG + tig0023091:81684-81703 None:intergenic 25.0%
TAAGCATGTTGTGGAAATTA+GGG - tig0023091:80190-80209 Msa1404870:CDS 30.0%
GAAGAATCATCACAGTTAAT+TGG + tig0023091:80297-80316 None:intergenic 30.0%
! CTGGTTTAGGCTAAATATTT+TGG - tig0023091:80462-80481 Msa1404870:intron 30.0%
AATATACAAGCATGCACATA+GGG + tig0023091:80531-80550 None:intergenic 30.0%
ACAATACATTAGTGCATAGT+TGG - tig0023091:80616-80635 Msa1404870:intron 30.0%
TATCCAAGCATATACTAAGA+AGG - tig0023091:80724-80743 Msa1404870:intron 30.0%
!! CTTTTCAAGACACAGAAAAA+CGG + tig0023091:80814-80833 None:intergenic 30.0%
GACAACAGAATAAAATACCA+AGG + tig0023091:80954-80973 None:intergenic 30.0%
!!! CTTTTGTTTTCTTTGTAAGC+TGG + tig0023091:81204-81223 None:intergenic 30.0%
TTAGTAGGAAATTGGTTATG+TGG - tig0023091:81509-81528 Msa1404870:intron 30.0%
TAAGCATGTTGTGGAAATTA+GGG - tig0023091:80190-80209 Msa1404870:CDS 30.0%
GAAGAATCATCACAGTTAAT+TGG + tig0023091:80297-80316 None:intergenic 30.0%
! CTGGTTTAGGCTAAATATTT+TGG - tig0023091:80462-80481 Msa1404870:intron 30.0%
AATATACAAGCATGCACATA+GGG + tig0023091:80531-80550 None:intergenic 30.0%
ACAATACATTAGTGCATAGT+TGG - tig0023091:80616-80635 Msa1404870:intron 30.0%
TATCCAAGCATATACTAAGA+AGG - tig0023091:80724-80743 Msa1404870:intron 30.0%
!! CTTTTCAAGACACAGAAAAA+CGG + tig0023091:80814-80833 None:intergenic 30.0%
GACAACAGAATAAAATACCA+AGG + tig0023091:80954-80973 None:intergenic 30.0%
!!! CTTTTGTTTTCTTTGTAAGC+TGG + tig0023091:81204-81223 None:intergenic 30.0%
TTAGTAGGAAATTGGTTATG+TGG - tig0023091:81509-81528 Msa1404870:intron 30.0%
AGTTCCACAAAACCCTAAAA+CGG - tig0023091:79866-79885 Msa1404870:three_prime_UTR 35.0%
GTCAACAACAACAATAGGTT+TGG + tig0023091:79955-79974 None:intergenic 35.0%
AACATGCTTACAGAGTTAAC+TGG + tig0023091:80180-80199 None:intergenic 35.0%
TTAACTCTGTAAGCATGTTG+TGG - tig0023091:80181-80200 Msa1404870:CDS 35.0%
GTAAGCATGTTGTGGAAATT+AGG - tig0023091:80189-80208 Msa1404870:CDS 35.0%
TTCAGTGGTATCATCTAACA+AGG + tig0023091:80245-80264 None:intergenic 35.0%
TGTTAGATGATACCACTGAA+TGG - tig0023091:80245-80264 Msa1404870:CDS 35.0%
TAACAAATGGAACCATTCAG+TGG + tig0023091:80260-80279 None:intergenic 35.0%
AGCACAGATACAACAATCAA+GGG + tig0023091:80380-80399 None:intergenic 35.0%
!!! CTTTTTATTTCTACCACGGT+TGG - tig0023091:80438-80457 Msa1404870:CDS 35.0%
CAATATACAAGCATGCACAT+AGG + tig0023091:80532-80551 None:intergenic 35.0%
GCATGCTTGTATATTGTTTG+TGG - tig0023091:80536-80555 Msa1404870:intron 35.0%
TTAGTGCATAGTTGGATTTG+CGG - tig0023091:80624-80643 Msa1404870:intron 35.0%
CTTCCTTCTTAGTATATGCT+TGG + tig0023091:80730-80749 None:intergenic 35.0%
! CTAGCGATCTTTTATAGCAT+AGG + tig0023091:80781-80800 None:intergenic 35.0%
TGTGTTCATCTATATGTCAC+AGG - tig0023091:80881-80900 Msa1404870:intron 35.0%
CTATATGTCACAGGTTTAGT+TGG - tig0023091:80890-80909 Msa1404870:intron 35.0%
!! AACATATGCGACTGTTTTGA+AGG - tig0023091:81101-81120 Msa1404870:intron 35.0%
ATCCTTGTTAAATCCTTGTC+AGG - tig0023091:81148-81167 Msa1404870:intron 35.0%
AACCTGACAAGGATTTAACA+AGG + tig0023091:81153-81172 None:intergenic 35.0%
AAGTGATACAGAAGATGATG+AGG - tig0023091:81221-81240 Msa1404870:intron 35.0%
TTGTGTCAAGCTTAGTGATT+AGG - tig0023091:81539-81558 Msa1404870:CDS 35.0%
TCATTGACATAACTTGACAC+AGG + tig0023091:81595-81614 None:intergenic 35.0%
ATTGCTTGTGCTATCAATCT+TGG - tig0023091:81721-81740 Msa1404870:five_prime_UTR 35.0%
AGTTCCACAAAACCCTAAAA+CGG - tig0023091:79866-79885 Msa1404870:three_prime_UTR 35.0%
GTCAACAACAACAATAGGTT+TGG + tig0023091:79955-79974 None:intergenic 35.0%
AACATGCTTACAGAGTTAAC+TGG + tig0023091:80180-80199 None:intergenic 35.0%
TTAACTCTGTAAGCATGTTG+TGG - tig0023091:80181-80200 Msa1404870:CDS 35.0%
GTAAGCATGTTGTGGAAATT+AGG - tig0023091:80189-80208 Msa1404870:CDS 35.0%
TTCAGTGGTATCATCTAACA+AGG + tig0023091:80245-80264 None:intergenic 35.0%
TGTTAGATGATACCACTGAA+TGG - tig0023091:80245-80264 Msa1404870:CDS 35.0%
TAACAAATGGAACCATTCAG+TGG + tig0023091:80260-80279 None:intergenic 35.0%
AGCACAGATACAACAATCAA+GGG + tig0023091:80380-80399 None:intergenic 35.0%
!!! CTTTTTATTTCTACCACGGT+TGG - tig0023091:80438-80457 Msa1404870:CDS 35.0%
CAATATACAAGCATGCACAT+AGG + tig0023091:80532-80551 None:intergenic 35.0%
GCATGCTTGTATATTGTTTG+TGG - tig0023091:80536-80555 Msa1404870:intron 35.0%
TTAGTGCATAGTTGGATTTG+CGG - tig0023091:80624-80643 Msa1404870:intron 35.0%
CTTCCTTCTTAGTATATGCT+TGG + tig0023091:80730-80749 None:intergenic 35.0%
! CTAGCGATCTTTTATAGCAT+AGG + tig0023091:80781-80800 None:intergenic 35.0%
TGTGTTCATCTATATGTCAC+AGG - tig0023091:80881-80900 Msa1404870:intron 35.0%
CTATATGTCACAGGTTTAGT+TGG - tig0023091:80890-80909 Msa1404870:intron 35.0%
!! AACATATGCGACTGTTTTGA+AGG - tig0023091:81101-81120 Msa1404870:intron 35.0%
ATCCTTGTTAAATCCTTGTC+AGG - tig0023091:81148-81167 Msa1404870:intron 35.0%
AACCTGACAAGGATTTAACA+AGG + tig0023091:81153-81172 None:intergenic 35.0%
AAGTGATACAGAAGATGATG+AGG - tig0023091:81221-81240 Msa1404870:intron 35.0%
TTGTGTCAAGCTTAGTGATT+AGG - tig0023091:81539-81558 Msa1404870:CDS 35.0%
TCATTGACATAACTTGACAC+AGG + tig0023091:81595-81614 None:intergenic 35.0%
ATTGCTTGTGCTATCAATCT+TGG - tig0023091:81721-81740 Msa1404870:five_prime_UTR 35.0%
! TCAAGTACTCCACCGTTTTA+GGG + tig0023091:79881-79900 None:intergenic 40.0%
GAGGAGTCAACAACAACAAT+AGG + tig0023091:79960-79979 None:intergenic 40.0%
GAGAATTGGAAGGTGAGATT+GGG + tig0023091:80012-80031 None:intergenic 40.0%
AGAGAATTGGAAGGTGAGAT+TGG + tig0023091:80013-80032 None:intergenic 40.0%
CAGCACAGATACAACAATCA+AGG + tig0023091:80381-80400 None:intergenic 40.0%
AGACACAGAAAAACGGACAA+GGG + tig0023091:80807-80826 None:intergenic 40.0%
AAGACACAGAAAAACGGACA+AGG + tig0023091:80808-80827 None:intergenic 40.0%
!! GTTTGTTTTTCTCCCCAGAT+TGG - tig0023091:81005-81024 Msa1404870:intron 40.0%
AGTCAGCAGATATCCAATCT+GGG + tig0023091:81021-81040 None:intergenic 40.0%
TAGTCAGCAGATATCCAATC+TGG + tig0023091:81022-81041 None:intergenic 40.0%
GAATCCATTGCAGTCAACTT+TGG + tig0023091:81065-81084 None:intergenic 40.0%
! TGATGATGACGTACAAGATG+AGG - tig0023091:81254-81273 Msa1404870:intron 40.0%
GTACAAGATGAGGATGATGA+TGG - tig0023091:81264-81283 Msa1404870:intron 40.0%
GATGAGGAAGATGAAGAAGA+TGG - tig0023091:81402-81421 Msa1404870:intron 40.0%
TGACACAGGTTCACTTACTA+AGG + tig0023091:81581-81600 None:intergenic 40.0%
CACTCACGGACAATAATAAC+TGG + tig0023091:81670-81689 None:intergenic 40.0%
! TCAAGTACTCCACCGTTTTA+GGG + tig0023091:79881-79900 None:intergenic 40.0%
GAGGAGTCAACAACAACAAT+AGG + tig0023091:79960-79979 None:intergenic 40.0%
GAGAATTGGAAGGTGAGATT+GGG + tig0023091:80012-80031 None:intergenic 40.0%
AGAGAATTGGAAGGTGAGAT+TGG + tig0023091:80013-80032 None:intergenic 40.0%
CAGCACAGATACAACAATCA+AGG + tig0023091:80381-80400 None:intergenic 40.0%
AGACACAGAAAAACGGACAA+GGG + tig0023091:80807-80826 None:intergenic 40.0%
AAGACACAGAAAAACGGACA+AGG + tig0023091:80808-80827 None:intergenic 40.0%
!! GTTTGTTTTTCTCCCCAGAT+TGG - tig0023091:81005-81024 Msa1404870:intron 40.0%
AGTCAGCAGATATCCAATCT+GGG + tig0023091:81021-81040 None:intergenic 40.0%
TAGTCAGCAGATATCCAATC+TGG + tig0023091:81022-81041 None:intergenic 40.0%
GAATCCATTGCAGTCAACTT+TGG + tig0023091:81065-81084 None:intergenic 40.0%
! TGATGATGACGTACAAGATG+AGG - tig0023091:81254-81273 Msa1404870:intron 40.0%
GTACAAGATGAGGATGATGA+TGG - tig0023091:81264-81283 Msa1404870:intron 40.0%
GATGAGGAAGATGAAGAAGA+TGG - tig0023091:81402-81421 Msa1404870:intron 40.0%
TGACACAGGTTCACTTACTA+AGG + tig0023091:81581-81600 None:intergenic 40.0%
CACTCACGGACAATAATAAC+TGG + tig0023091:81670-81689 None:intergenic 40.0%
TCCACAAAACCCTAAAACGG+TGG - tig0023091:79869-79888 Msa1404870:three_prime_UTR 45.0%
!!! TCCACCGTTTTAGGGTTTTG+TGG + tig0023091:79873-79892 None:intergenic 45.0%
! CTCAAGTACTCCACCGTTTT+AGG + tig0023091:79882-79901 None:intergenic 45.0%
!! ACTTGAGTTGAGTTGAGTCC+AGG - tig0023091:79895-79914 Msa1404870:three_prime_UTR 45.0%
!! CTTGAGTTGAGTTGAGTCCA+GGG - tig0023091:79896-79915 Msa1404870:three_prime_UTR 45.0%
TGATTAGAGCGAGTAAACCC+TGG + tig0023091:79916-79935 None:intergenic 45.0%
CTAACAAGGCACAGAGTTCA+AGG + tig0023091:80231-80250 None:intergenic 45.0%
! TATTTCTACCACGGTTGGAC+TGG - tig0023091:80443-80462 Msa1404870:CDS 45.0%
AGTTGGATTTGCGGTGAGAT+TGG - tig0023091:80633-80652 Msa1404870:intron 45.0%
GTCAGCAGATATCCAATCTG+GGG + tig0023091:81020-81039 None:intergenic 45.0%
GACTAATGCTGCCAAATCAG+TGG - tig0023091:81037-81056 Msa1404870:intron 45.0%
!! CAACTTTGGAGCCACTGATT+TGG + tig0023091:81051-81070 None:intergenic 45.0%
AATCCTTGTCAGGTTCCCTT+TGG - tig0023091:81158-81177 Msa1404870:intron 45.0%
!! TTCCAAGAAGGTGCTCCAAA+GGG + tig0023091:81176-81195 None:intergenic 45.0%
!! TGAGGATGATGATGGAGAAG+AGG - tig0023091:81272-81291 Msa1404870:intron 45.0%
AGAAGATGGCGAAGATGAAG+AGG - tig0023091:81416-81435 Msa1404870:intron 45.0%
TCTCACTTCCTCTTCTTTGC+TGG + tig0023091:81483-81502 None:intergenic 45.0%
TCCACAAAACCCTAAAACGG+TGG - tig0023091:79869-79888 Msa1404870:three_prime_UTR 45.0%
!!! TCCACCGTTTTAGGGTTTTG+TGG + tig0023091:79873-79892 None:intergenic 45.0%
! CTCAAGTACTCCACCGTTTT+AGG + tig0023091:79882-79901 None:intergenic 45.0%
!! ACTTGAGTTGAGTTGAGTCC+AGG - tig0023091:79895-79914 Msa1404870:three_prime_UTR 45.0%
!! CTTGAGTTGAGTTGAGTCCA+GGG - tig0023091:79896-79915 Msa1404870:three_prime_UTR 45.0%
TGATTAGAGCGAGTAAACCC+TGG + tig0023091:79916-79935 None:intergenic 45.0%
CTAACAAGGCACAGAGTTCA+AGG + tig0023091:80231-80250 None:intergenic 45.0%
! TATTTCTACCACGGTTGGAC+TGG - tig0023091:80443-80462 Msa1404870:CDS 45.0%
AGTTGGATTTGCGGTGAGAT+TGG - tig0023091:80633-80652 Msa1404870:intron 45.0%
GTCAGCAGATATCCAATCTG+GGG + tig0023091:81020-81039 None:intergenic 45.0%
GACTAATGCTGCCAAATCAG+TGG - tig0023091:81037-81056 Msa1404870:intron 45.0%
!! CAACTTTGGAGCCACTGATT+TGG + tig0023091:81051-81070 None:intergenic 45.0%
AATCCTTGTCAGGTTCCCTT+TGG - tig0023091:81158-81177 Msa1404870:intron 45.0%
!! TTCCAAGAAGGTGCTCCAAA+GGG + tig0023091:81176-81195 None:intergenic 45.0%
!! TGAGGATGATGATGGAGAAG+AGG - tig0023091:81272-81291 Msa1404870:intron 45.0%
AGAAGATGGCGAAGATGAAG+AGG - tig0023091:81416-81435 Msa1404870:intron 45.0%
TCTCACTTCCTCTTCTTTGC+TGG + tig0023091:81483-81502 None:intergenic 45.0%
TGACTCCTCTCTCTCACTCT+CGG - tig0023091:79971-79990 Msa1404870:three_prime_UTR 50.0%
TACGAGAGCGAGAGATTGAG+CGG + tig0023091:80093-80112 None:intergenic 50.0%
!! TACCACGGTTGGACTGGTTT+AGG - tig0023091:80449-80468 Msa1404870:CDS 50.0%
GGCTCCAAAGTTGACTGCAA+TGG - tig0023091:81058-81077 Msa1404870:intron 50.0%
!! TTCCCTTTGGAGCACCTTCT+TGG - tig0023091:81171-81190 Msa1404870:intron 50.0%
!! CTTCCAAGAAGGTGCTCCAA+AGG + tig0023091:81177-81196 None:intergenic 50.0%
!! CTTTGGAGCACCTTCTTGGA+AGG - tig0023091:81175-81194 Msa1404870:intron 50.0%
! AAGCTGGCTTCCTTCCAAGA+AGG + tig0023091:81188-81207 None:intergenic 50.0%
GGAGAAGAGGAAGACTACTC+CGG - tig0023091:81285-81304 Msa1404870:intron 50.0%
GAAGACTACTCCGGTGATGA+AGG - tig0023091:81294-81313 Msa1404870:intron 50.0%
TTCTTCCTCGCCTTCATCAC+CGG + tig0023091:81307-81326 None:intergenic 50.0%
GTGATGAAGGCGAGGAAGAA+GGG - tig0023091:81307-81326 Msa1404870:intron 50.0%
TGATGAAGGCGAGGAAGAAG+GGG - tig0023091:81308-81327 Msa1404870:intron 50.0%
TTAGCCTCAGGATCATCCTC+GGG + tig0023091:81336-81355 None:intergenic 50.0%
! GTTAGCCTCAGGATCATCCT+CGG + tig0023091:81337-81356 None:intergenic 50.0%
GAGGATGATCCTGAGGCTAA+CGG - tig0023091:81336-81355 Msa1404870:intron 50.0%
GTGCAGGAGGAAGTGATGAT+GGG - tig0023091:81358-81377 Msa1404870:intron 50.0%
! TGCAGGAGGAAGTGATGATG+GGG - tig0023091:81359-81378 Msa1404870:intron 50.0%
! GGGGAAGATGATGATGACGA+TGG - tig0023091:81378-81397 Msa1404870:intron 50.0%
! TGATGATGACGATGGCGATG+AGG - tig0023091:81386-81405 Msa1404870:intron 50.0%
CGAAGATGAAGAGGACGAAG+AGG - tig0023091:81425-81444 Msa1404870:intron 50.0%
AGATGAAGAGGACGAAGAGG+AGG - tig0023091:81428-81447 Msa1404870:intron 50.0%
CACTTCCTCTTCTTTGCTGG+TGG + tig0023091:81480-81499 None:intergenic 50.0%
TGACTCCTCTCTCTCACTCT+CGG - tig0023091:79971-79990 Msa1404870:three_prime_UTR 50.0%
TACGAGAGCGAGAGATTGAG+CGG + tig0023091:80093-80112 None:intergenic 50.0%
!! TACCACGGTTGGACTGGTTT+AGG - tig0023091:80449-80468 Msa1404870:CDS 50.0%
GGCTCCAAAGTTGACTGCAA+TGG - tig0023091:81058-81077 Msa1404870:intron 50.0%
!! TTCCCTTTGGAGCACCTTCT+TGG - tig0023091:81171-81190 Msa1404870:intron 50.0%
!! CTTCCAAGAAGGTGCTCCAA+AGG + tig0023091:81177-81196 None:intergenic 50.0%
!! CTTTGGAGCACCTTCTTGGA+AGG - tig0023091:81175-81194 Msa1404870:intron 50.0%
! AAGCTGGCTTCCTTCCAAGA+AGG + tig0023091:81188-81207 None:intergenic 50.0%
GGAGAAGAGGAAGACTACTC+CGG - tig0023091:81285-81304 Msa1404870:intron 50.0%
GAAGACTACTCCGGTGATGA+AGG - tig0023091:81294-81313 Msa1404870:intron 50.0%
TTCTTCCTCGCCTTCATCAC+CGG + tig0023091:81307-81326 None:intergenic 50.0%
GTGATGAAGGCGAGGAAGAA+GGG - tig0023091:81307-81326 Msa1404870:intron 50.0%
TGATGAAGGCGAGGAAGAAG+GGG - tig0023091:81308-81327 Msa1404870:intron 50.0%
TTAGCCTCAGGATCATCCTC+GGG + tig0023091:81336-81355 None:intergenic 50.0%
! GTTAGCCTCAGGATCATCCT+CGG + tig0023091:81337-81356 None:intergenic 50.0%
GAGGATGATCCTGAGGCTAA+CGG - tig0023091:81336-81355 Msa1404870:intron 50.0%
GTGCAGGAGGAAGTGATGAT+GGG - tig0023091:81358-81377 Msa1404870:intron 50.0%
! TGCAGGAGGAAGTGATGATG+GGG - tig0023091:81359-81378 Msa1404870:intron 50.0%
! GGGGAAGATGATGATGACGA+TGG - tig0023091:81378-81397 Msa1404870:intron 50.0%
! TGATGATGACGATGGCGATG+AGG - tig0023091:81386-81405 Msa1404870:intron 50.0%
CGAAGATGAAGAGGACGAAG+AGG - tig0023091:81425-81444 Msa1404870:intron 50.0%
AGATGAAGAGGACGAAGAGG+AGG - tig0023091:81428-81447 Msa1404870:intron 50.0%
CACTTCCTCTTCTTTGCTGG+TGG + tig0023091:81480-81499 None:intergenic 50.0%
CATTGCCGAGAGTGAGAGAG+AGG + tig0023091:79979-79998 None:intergenic 55.0%
CTCTCTCTCACTCTCGGCAA+TGG - tig0023091:79977-79996 Msa1404870:three_prime_UTR 55.0%
CGACGACGGAGAGAATTGGA+AGG + tig0023091:80022-80041 None:intergenic 55.0%
AGGTCGACGACGGAGAGAAT+TGG + tig0023091:80026-80045 None:intergenic 55.0%
CGACAGCTTGGAGAAGAGAG+GGG + tig0023091:80049-80068 None:intergenic 55.0%
GCGACAGCTTGGAGAAGAGA+GGG + tig0023091:80050-80069 None:intergenic 55.0%
!! GGCGGAAACAAGAGTGTCGA+CGG + tig0023091:80072-80091 None:intergenic 55.0%
AGCCTAAACCAGTCCAACCG+TGG + tig0023091:80454-80473 None:intergenic 55.0%
GCTCCAAAGGGAACCTGACA+AGG + tig0023091:81164-81183 None:intergenic 55.0%
GGTGATGAAGGCGAGGAAGA+AGG - tig0023091:81306-81325 Msa1404870:intron 55.0%
GGTGCAGGAGGAAGTGATGA+TGG - tig0023091:81357-81376 Msa1404870:intron 55.0%
AGAGGACGAAGAGGAGGAAG+AGG - tig0023091:81434-81453 Msa1404870:intron 55.0%
CTCTTCTTTGCTGGTGGCTG+GGG + tig0023091:81474-81493 None:intergenic 55.0%
CCTCTTCTTTGCTGGTGGCT+GGG + tig0023091:81475-81494 None:intergenic 55.0%
TCCTCTTCTTTGCTGGTGGC+TGG + tig0023091:81476-81495 None:intergenic 55.0%
CATTGCCGAGAGTGAGAGAG+AGG + tig0023091:79979-79998 None:intergenic 55.0%
CTCTCTCTCACTCTCGGCAA+TGG - tig0023091:79977-79996 Msa1404870:three_prime_UTR 55.0%
CGACGACGGAGAGAATTGGA+AGG + tig0023091:80022-80041 None:intergenic 55.0%
AGGTCGACGACGGAGAGAAT+TGG + tig0023091:80026-80045 None:intergenic 55.0%
CGACAGCTTGGAGAAGAGAG+GGG + tig0023091:80049-80068 None:intergenic 55.0%
GCGACAGCTTGGAGAAGAGA+GGG + tig0023091:80050-80069 None:intergenic 55.0%
!! GGCGGAAACAAGAGTGTCGA+CGG + tig0023091:80072-80091 None:intergenic 55.0%
AGCCTAAACCAGTCCAACCG+TGG + tig0023091:80454-80473 None:intergenic 55.0%
GCTCCAAAGGGAACCTGACA+AGG + tig0023091:81164-81183 None:intergenic 55.0%
GGTGATGAAGGCGAGGAAGA+AGG - tig0023091:81306-81325 Msa1404870:intron 55.0%
GGTGCAGGAGGAAGTGATGA+TGG - tig0023091:81357-81376 Msa1404870:intron 55.0%
AGAGGACGAAGAGGAGGAAG+AGG - tig0023091:81434-81453 Msa1404870:intron 55.0%
CTCTTCTTTGCTGGTGGCTG+GGG + tig0023091:81474-81493 None:intergenic 55.0%
CCTCTTCTTTGCTGGTGGCT+GGG + tig0023091:81475-81494 None:intergenic 55.0%
TCCTCTTCTTTGCTGGTGGC+TGG + tig0023091:81476-81495 None:intergenic 55.0%
AAGAGAGGGGAGGTCGACGA+CGG + tig0023091:80036-80055 None:intergenic 60.0%
CAGCTTGGAGAAGAGAGGGG+AGG + tig0023091:80046-80065 None:intergenic 60.0%
GGCGACAGCTTGGAGAAGAG+AGG + tig0023091:80051-80070 None:intergenic 60.0%
GAGAGCGAGAGATTGAGCGG+CGG + tig0023091:80090-80109 None:intergenic 60.0%
CTACTCCGGTGATGAAGGCG+AGG - tig0023091:81299-81318 Msa1404870:intron 60.0%
GGATCCCGAGGATGATCCTG+AGG - tig0023091:81329-81348 Msa1404870:intron 60.0%
GATCCTGAGGCTAACGGTGC+AGG - tig0023091:81342-81361 Msa1404870:intron 60.0%
CCCAGCCACCAGCAAAGAAG+AGG - tig0023091:81472-81491 Msa1404870:intron 60.0%
AAGAGAGGGGAGGTCGACGA+CGG + tig0023091:80036-80055 None:intergenic 60.0%
CAGCTTGGAGAAGAGAGGGG+AGG + tig0023091:80046-80065 None:intergenic 60.0%
GGCGACAGCTTGGAGAAGAG+AGG + tig0023091:80051-80070 None:intergenic 60.0%
GAGAGCGAGAGATTGAGCGG+CGG + tig0023091:80090-80109 None:intergenic 60.0%
CTACTCCGGTGATGAAGGCG+AGG - tig0023091:81299-81318 Msa1404870:intron 60.0%
GGATCCCGAGGATGATCCTG+AGG - tig0023091:81329-81348 Msa1404870:intron 60.0%
GATCCTGAGGCTAACGGTGC+AGG - tig0023091:81342-81361 Msa1404870:intron 60.0%
CCCAGCCACCAGCAAAGAAG+AGG - tig0023091:81472-81491 Msa1404870:intron 60.0%
!! GAGTGTCGACGGCGACAGCT+TGG + tig0023091:80061-80080 None:intergenic 65.0%
CGAGGAAGAAGGGGATCCCG+AGG - tig0023091:81317-81336 Msa1404870:intron 65.0%
! CCTCCTGCACCGTTAGCCTC+AGG + tig0023091:81348-81367 None:intergenic 65.0%
CCTGAGGCTAACGGTGCAGG+AGG - tig0023091:81345-81364 Msa1404870:intron 65.0%
!! GAGTGTCGACGGCGACAGCT+TGG + tig0023091:80061-80080 None:intergenic 65.0%
CGAGGAAGAAGGGGATCCCG+AGG - tig0023091:81317-81336 Msa1404870:intron 65.0%
! CCTCCTGCACCGTTAGCCTC+AGG + tig0023091:81348-81367 None:intergenic 65.0%
CCTGAGGCTAACGGTGCAGG+AGG - tig0023091:81345-81364 Msa1404870:intron 65.0%
Chromosome Type Strat End Strand Name
tig0023091 gene 79863 81777 79863 ID=Msa1404870;Name=Msa1404870
tig0023091 mRNA 79863 81777 79863 ID=Msa1404870-mRNA-1;Parent=Msa1404870;Name=Msa1404870-mRNA-1;_AED=0.00;_eAED=0.00;_QI=133|1|1|1|1|1|3|277|169
tig0023091 exon 79863 80470 79863 ID=Msa1404870-mRNA-1:exon:23811;Parent=Msa1404870-mRNA-1
tig0023091 exon 80553 80617 80553 ID=Msa1404870-mRNA-1:exon:23810;Parent=Msa1404870-mRNA-1
tig0023091 exon 81531 81777 81531 ID=Msa1404870-mRNA-1:exon:23809;Parent=Msa1404870-mRNA-1
tig0023091 five_prime_UTR 81645 81777 81645 ID=Msa1404870-mRNA-1:five_prime_utr;Parent=Msa1404870-mRNA-1
tig0023091 CDS 81531 81644 81531 ID=Msa1404870-mRNA-1:cds;Parent=Msa1404870-mRNA-1
tig0023091 CDS 80553 80617 80553 ID=Msa1404870-mRNA-1:cds;Parent=Msa1404870-mRNA-1
tig0023091 CDS 80140 80470 80140 ID=Msa1404870-mRNA-1:cds;Parent=Msa1404870-mRNA-1
tig0023091 three_prime_UTR 79863 80139 79863 ID=Msa1404870-mRNA-1:three_prime_utr;Parent=Msa1404870-mRNA-1
Gene Sequence

>Msa1404870

ATGGCTGTGTCAACCCAATCTCACCTTCCAATTCTCTCCGTCGTCGACCTCCCCTCTCTTCTCCAAGCTGTCGCCGTCGACACTCTTGTTTCCGCCGCTCAATCTCTCGCTCTCATTGGATATCTGCTGACTAATGCTGCCAAATCAGTGGCTCCAAAGTTGACTGCAATGGATTCAATGTTCCCTTTGGAGCACCTTCTTGGAAGGAAGCCAGCTTACAAAGAAAACAAAAGTGATACAGAAGATGATGAGGACGACGACGATGATGATGACGTACAAGATGAGGATGATGATGGAGAAGAGGAAGACTACTCCGGTGATGAAGGCGAGGAAGAAGGGGATCCCGAGGATGATCCTGAGGCTAACGGTGCAGGAGGAAGTGATGATGGGGAAGATGATGATGACGATGGCGATGAGGAAGATGAAGAAGATGGCGAAGATGAAGAGGACGAAGAGGAGGAAGAGGAAGATGATGAGACTCCCCAGCCACCAGCAAAGAAGAGGAAGTGA
Protein sequence

>Msa1404870

MAVSTQSHLPILSVVDLPSLLQAVAVDTLVSAAQSLALIGYLLTNAAKSVAPKLTAMDSMFPLEHLLGRKPAYKENKSDTEDDEDDDDDDDVQDEDDDGEEEDYSGDEGEEEGDPEDDPEANGAGGSDDGEDDDDDGDEEDEEDGEDEEDEEEEEEDDETPQPPAKKRK*