AlfalfaGEDB | Msa0727030

M. sativa cultivar zhongmu-4 / Msa0727030

KEGG: K02108
GO: GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0016020 GO:0016462 GO:0016469 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031976 GO:0031984 GO:0032991 GO:0033177 GO:0034220 GO:0034357 GO:0042623 GO:0042625 GO:0042626 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045259 GO:0045263 GO:0046933 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0099131 GO:0099132 GO:1902600
PFAM: PF00119.23

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0727030	YP_010173352.1	92.523	107	8	0	6	112	1	107	7.70e-64	204

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0727030	sp\|P06452\|ATPI_PEA	92.523	107	8	0	6	112	1	107	9.83e-67	204

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0727030	A0A898N9N6	92.523	107	8	0	6	112	1	107	3.68e-64	204

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0095140	Msa0727030	0.816965	4.212817e-52	-8.615850e-47
Msa0095270	Msa0727030	0.821848	3.245655e-53	-8.615850e-47
Msa0727030	Msa1157160	0.808366	3.208621e-50	-8.615850e-47
Msa0727030	Msa1355020	0.805691	1.181312e-49	-8.615850e-47
Msa0727030	Msa1394090	0.801625	8.244392e-49	-8.615850e-47
Msa0727030	Msa1394100	0.804761	1.849661e-49	-8.615850e-47
Msa0139740	Msa0727030	0.806642	7.448453e-50	-8.615850e-47
Msa0221860	Msa0727030	0.810831	9.476633e-51	-8.615850e-47
Msa0427050	Msa0727030	0.806911	6.535332e-50	-8.615850e-47
Msa0475830	Msa0727030	0.807156	5.800678e-50	-8.615850e-47
Msa0268340	Msa0727030	0.805329	1.406820e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0727030	MtrunA17_Chr4g0016701	89.720	107	9	1	6	112	1	105	6.05e-63	192
Msa0727030	MtrunA17_CPg0492981	89.720	107	9	1	6	112	1	105	6.05e-63	192
Msa0727030	MtrunA17_Chr4g0015611	89.720	107	9	1	6	112	1	105	6.05e-63	192
Msa0727030	MtrunA17_Chr3g0088491	88.136	59	7	0	54	112	16	74	2.03e-30	108

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0727030	ATCG00150.1	77.064	109	23	1	6	112	1	109	1.37e-54	171

Find 22 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACATTTCTATTGGCAAAT+AGG	0.196864	5_1:+28921749	Msa0727030:CDS
GGTCCGTGGGTTCCGTTTAT+TGG	0.198793	5_1:+28921953	Msa0727030:CDS
AACATTTCTATTGGCAAATA+GGG	0.237546	5_1:+28921750	Msa0727030:CDS
CTCAAGTACATATTACCTCT+TGG	0.383511	5_1:+28921789	Msa0727030:CDS
CTATTTGCATTTCCAATAAA+CGG	0.391860	5_1:-28921965	None:intergenic
CGGATTTCTAACAACTAGAA+TGG	0.400438	5_1:-28921840	None:intergenic
ACTCAGATTGGAGAAGAATA+TGG	0.441550	5_1:+28921932	Msa0727030:CDS
CCGGACGTTGGAATAGTTTG+CGG	0.455395	5_1:-28921860	None:intergenic
AAGTAGGTCAACATTTCTAT+TGG	0.460455	5_1:+28921741	Msa0727030:CDS
TCAAGTACATATTACCTCTT+GGG	0.467262	5_1:+28921790	Msa0727030:CDS
TCACGTCTCGGATGAATTCA+AGG	0.477047	5_1:-28921904	None:intergenic
CGGTCATAGATTTATGGAAT+CGG	0.488490	5_1:+28921498	None:intergenic
GATATATCTGCTGTCGAAGT+AGG	0.493921	5_1:+28921725	Msa0727030:CDS
GACGTGAGCAAAACTCAGAT+TGG	0.510359	5_1:+28921920	Msa0727030:CDS
CCGCAAACTATTCCAACGTC+CGG	0.541897	5_1:+28921860	Msa0727030:CDS
AAGAAATTCTGACCGGACGT+TGG	0.546490	5_1:-28921872	None:intergenic
ACATTTCTATTGGCAAATAG+GGG	0.561596	5_1:+28921751	Msa0727030:CDS
TGGAGAAGAATATGGTCCGT+GGG	0.620080	5_1:+28921940	Msa0727030:CDS
TTGGAGAAGAATATGGTCCG+TGG	0.620994	5_1:+28921939	Msa0727030:CDS
ATATTCAAAGAAATTCTGAC+CGG	0.632509	5_1:-28921879	None:intergenic
TTTCCAATAAACGGAACCCA+CGG	0.651361	5_1:-28921956	None:intergenic
GATAGCAATTACAACCCAAG+AGG	0.659110	5_1:-28921804	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATATATATATAACTTGAAA+GGG	-	chr5_1:28921635-28921654	None:intergenic	10.0%
!!	ATATATATATATAACTTGAA+AGG	-	chr5_1:28921636-28921655	None:intergenic	10.0%
!!!	ATAAATATTTTGTGATTAGT+AGG	+	chr5_1:28921550-28921569	Msa0727030:intron	15.0%
!!!	ATATTTTTTTTTTAGAGGAC+AGG	+	chr5_1:28921652-28921671	Msa0727030:intron	20.0%
!!!	TATTTTTTTTTTAGAGGACA+GGG	+	chr5_1:28921653-28921672	Msa0727030:intron	20.0%
!	GTAGGTATTCAAAATAGAAA+AGG	+	chr5_1:28921568-28921587	Msa0727030:intron	25.0%
!	AAGGAAATGAAAGTCAAATA+AGG	+	chr5_1:28921587-28921606	Msa0727030:intron	25.0%
!	ATGAAAGTCAAATAAGGAAA+TGG	+	chr5_1:28921593-28921612	Msa0727030:intron	25.0%
!	AACATTTCTATTGGCAAATA+GGG	+	chr5_1:28921750-28921769	Msa0727030:CDS	25.0%
!	ATATTCAAAGAAATTCTGAC+CGG	-	chr5_1:28921882-28921901	None:intergenic	25.0%
!	CTATTTGCATTTCCAATAAA+CGG	-	chr5_1:28921968-28921987	None:intergenic	25.0%
	AAGTAGGTCAACATTTCTAT+TGG	+	chr5_1:28921741-28921760	Msa0727030:CDS	30.0%
	CAACATTTCTATTGGCAAAT+AGG	+	chr5_1:28921749-28921768	Msa0727030:CDS	30.0%
	ACATTTCTATTGGCAAATAG+GGG	+	chr5_1:28921751-28921770	Msa0727030:CDS	30.0%
	TCAAGTACATATTACCTCTT+GGG	+	chr5_1:28921790-28921809	Msa0727030:CDS	30.0%
	CTCAAGTACATATTACCTCT+TGG	+	chr5_1:28921789-28921808	Msa0727030:CDS	35.0%
	CGGATTTCTAACAACTAGAA+TGG	-	chr5_1:28921843-28921862	None:intergenic	35.0%
	ACTCAGATTGGAGAAGAATA+TGG	+	chr5_1:28921932-28921951	Msa0727030:CDS	35.0%
	GATATATCTGCTGTCGAAGT+AGG	+	chr5_1:28921725-28921744	Msa0727030:CDS	40.0%
	GATAGCAATTACAACCCAAG+AGG	-	chr5_1:28921807-28921826	None:intergenic	40.0%
	TTTCCAATAAACGGAACCCA+CGG	-	chr5_1:28921959-28921978	None:intergenic	40.0%
	AAGAAATTCTGACCGGACGT+TGG	-	chr5_1:28921875-28921894	None:intergenic	45.0%
	TCACGTCTCGGATGAATTCA+AGG	-	chr5_1:28921907-28921926	None:intergenic	45.0%
!	TCTGAGTTTTGCTCACGTCT+CGG	-	chr5_1:28921919-28921938	None:intergenic	45.0%
	GACGTGAGCAAAACTCAGAT+TGG	+	chr5_1:28921920-28921939	Msa0727030:CDS	45.0%
	TTGGAGAAGAATATGGTCCG+TGG	+	chr5_1:28921939-28921958	Msa0727030:CDS	45.0%
	TGGAGAAGAATATGGTCCGT+GGG	+	chr5_1:28921940-28921959	Msa0727030:CDS	45.0%
!!!	TATATATATTTTTTTTTTAG+AGG	+	chr5_1:28921647-28921666	Msa0727030:intron	5.0%
	CCGGACGTTGGAATAGTTTG+CGG	-	chr5_1:28921863-28921882	None:intergenic	50.0%
	CCGCAAACTATTCCAACGTC+CGG	+	chr5_1:28921860-28921879	Msa0727030:CDS	50.0%
	GGTCCGTGGGTTCCGTTTAT+TGG	+	chr5_1:28921953-28921972	Msa0727030:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	28921511	28922039	28921511	ID=Msa0727030;Name=Msa0727030
chr5_1	mRNA	28921511	28922039	28921511	ID=Msa0727030-mRNA-1;Parent=Msa0727030;Name=Msa0727030-mRNA-1;_AED=0.52;_eAED=0.52;_QI=0\|0\|0\|0.5\|0\|0\|2\|0\|124
chr5_1	exon	28921511	28921519	28921511	ID=Msa0727030-mRNA-1:exon:9967;Parent=Msa0727030-mRNA-1
chr5_1	exon	28921674	28922039	28921674	ID=Msa0727030-mRNA-1:exon:9968;Parent=Msa0727030-mRNA-1
chr5_1	CDS	28921511	28921519	28921511	ID=Msa0727030-mRNA-1:cds;Parent=Msa0727030-mRNA-1
chr5_1	CDS	28921674	28922039	28921674	ID=Msa0727030-mRNA-1:cds;Parent=Msa0727030-mRNA-1

Gene Sequence	>Msa0727030 ATGGAATCGGGCAATATGAATGTTCTATTATGTTACATCAACACATTAAACAGATTCTATGATATATCTGCTGTCGAAGTAGGTCAACATTTCTATTGGCAAATAGGGGATTTTCAAGTGCATGCTCAAGTACATATTACCTCTTGGGTTGTAATTGCTATCTTATTAATTTCAACCATTCTAGTTGTTAGAAATCCGCAAACTATTCCAACGTCCGGTCAGAATTTCTTTGAATATGTCCTTGAATTCATCCGAGACGTGAGCAAAACTCAGATTGGAGAAGAATATGGTCCGTGGGTTCCGTTTATTGGAAATGCAAATAGCTATATCATAATCATCACTCGTGAGTTGTATGATAAAATAGAATTCATATGA
Protein sequence	>Msa0727030 MESGNMNVLLCYINTLNRFYDISAVEVGQHFYWQIGDFQVHAQVHITSWVVIAILLISTILVVRNPQTIPTSGQNFFEYVLEFIRDVSKTQIGEEYGPWVPFIGNANSYIIIITRELYDKIEFI*