AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0221860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221860 MtrunA17_Chr4g0016881 100.000 30 0 0 1 30 1 30 4.06e-15 63.2
Msa0221860 MtrunA17_CPg0492791 100.000 30 0 0 1 30 1 30 4.99e-15 62.8
Msa0221860 MtrunA17_Chr4g0015781 100.000 30 0 0 1 30 1 30 4.99e-15 62.8
Msa0221860 MtrunA17_Chr4g0028951 100.000 30 0 0 1 30 1 30 4.99e-15 62.8
Msa0221860 MtrunA17_Chr8g0355611 100.000 30 0 0 1 30 1 30 4.99e-15 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221860 ATCG00630.1 90.000 30 3 0 1 30 1 30 1.73e-12 57.0

Find 14 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGTGTTAATAAGGAATCT+AGG 0.329597 2_1:+70650685 Msa0221860:CDS
ACCTAATAAAAGATTGGATC+GGG 0.419736 2_1:-70651104 None:intergenic
CACCAGTGGCAAGTACTCTA+TGG 0.426592 2_1:+70650008 Msa0221860:CDS
GAGTGTTAATAAGGAATCTA+GGG 0.452009 2_1:+70650686 Msa0221860:CDS
AGAACAACCTAATAAAAGAT+TGG 0.459837 2_1:-70651110 None:intergenic
CTATGGTTCGCGGTTCTAGC+AGG 0.487956 2_1:+70650025 Msa0221860:CDS
GGCAAGTACTCTATGGTTCG+CGG 0.527936 2_1:+70650015 Msa0221860:CDS
AACCTAATAAAAGATTGGAT+CGG 0.529575 2_1:-70651105 None:intergenic
ATATCTCTCTGTGGCACCAG+TGG 0.585814 2_1:+70649994 Msa0221860:CDS
GAGTCGCTTAGATTTATCCA+TGG 0.585856 2_1:-70651043 None:intergenic
AACCATAGAGTACTTGCCAC+TGG 0.586013 2_1:-70650010 None:intergenic
GTATAGGAATAAACAAACCA+TGG 0.629025 2_1:+70651026 Msa0221860:CDS
AGTGTTAATAAGGAATCTAG+GGG 0.667965 2_1:+70650687 Msa0221860:CDS
CCTAATAAAAGATTGGATCG+GGG 0.685110 2_1:-70651103 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATAAAAATAAAATTAAT+TGG + chr2_1:70650477-70650496 Msa0221860:intron 0.0%
!! TATATAAAAATAAAATTAAT+TGG + chr2_1:70650477-70650496 Msa0221860:intron 0.0%
!! AATAACTAGAATCAAAAAAA+GGG - chr2_1:70650097-70650116 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG + chr2_1:70650221-70650240 Msa0221860:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG - chr2_1:70650315-70650334 None:intergenic 15.0%
!!! AATCCTTTTTTTATAAGAAA+TGG + chr2_1:70650995-70651014 Msa0221860:intron 15.0%
!!! ATCCTTTTTTTATAAGAAAT+GGG + chr2_1:70650996-70651015 Msa0221860:intron 15.0%
!! AATAACTAGAATCAAAAAAA+GGG - chr2_1:70650097-70650116 None:intergenic 15.0%
!! AGAGCAAATTAATTTAATAA+AGG + chr2_1:70650221-70650240 Msa0221860:intron 15.0%
!! TATTATTAAATTCATAGCAA+TGG - chr2_1:70650315-70650334 None:intergenic 15.0%
!!! AATCCTTTTTTTATAAGAAA+TGG + chr2_1:70650995-70651014 Msa0221860:intron 15.0%
!!! ATCCTTTTTTTATAAGAAAT+GGG + chr2_1:70650996-70651015 Msa0221860:intron 15.0%
!! ATAACTAGAATCAAAAAAAG+GGG - chr2_1:70650096-70650115 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG - chr2_1:70650098-70650117 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG + chr2_1:70650096-70650115 Msa0221860:intron 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG - chr2_1:70650151-70650170 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG + chr2_1:70650224-70650243 Msa0221860:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG + chr2_1:70650230-70650249 Msa0221860:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG - chr2_1:70650346-70650365 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG + chr2_1:70650641-70650660 Msa0221860:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr2_1:70650677-70650696 Msa0221860:CDS 20.0%
!! AGATAAAGAAATAGAGAAAA+TGG + chr2_1:70650811-70650830 Msa0221860:intron 20.0%
!! ATCCCATTTCTTATAAAAAA+AGG - chr2_1:70651001-70651020 None:intergenic 20.0%
!!! TTTATAAGAAATGGGATTTT+CGG + chr2_1:70651004-70651023 Msa0221860:intron 20.0%
!! ATAACTAGAATCAAAAAAAG+GGG - chr2_1:70650096-70650115 None:intergenic 20.0%
!! CAATAACTAGAATCAAAAAA+AGG - chr2_1:70650098-70650117 None:intergenic 20.0%
!!! CTTTTTTTGATTCTAGTTAT+TGG + chr2_1:70650096-70650115 Msa0221860:intron 20.0%
!!! AGAGGGATTTAATATTTTAT+TGG - chr2_1:70650151-70650170 None:intergenic 20.0%
!! GCAAATTAATTTAATAAAGG+TGG + chr2_1:70650224-70650243 Msa0221860:intron 20.0%
!!! TAATTTAATAAAGGTGGATT+TGG + chr2_1:70650230-70650249 Msa0221860:intron 20.0%
!! TTCTATTACAATTCCATTTA+TGG - chr2_1:70650346-70650365 None:intergenic 20.0%
!!! TATACGAATAACTGTTATTT+TGG + chr2_1:70650641-70650660 Msa0221860:intron 20.0%
!!! TGATAAAAAGAGTGTTAATA+AGG + chr2_1:70650677-70650696 Msa0221860:CDS 20.0%
!! AGATAAAGAAATAGAGAAAA+TGG + chr2_1:70650811-70650830 Msa0221860:intron 20.0%
!! ATCCCATTTCTTATAAAAAA+AGG - chr2_1:70651001-70651020 None:intergenic 20.0%
!!! TTTATAAGAAATGGGATTTT+CGG + chr2_1:70651004-70651023 Msa0221860:intron 20.0%
! AAAAAAAAAACGAACGAAGA+GGG - chr2_1:70650168-70650187 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG - chr2_1:70650169-70650188 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG + chr2_1:70650350-70650369 Msa0221860:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG + chr2_1:70650351-70650370 Msa0221860:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG + chr2_1:70650595-70650614 Msa0221860:intron 25.0%
! TTATAGAATTTACCTCCTAA+GGG - chr2_1:70650852-70650871 None:intergenic 25.0%
! TTTATAGAATTTACCTCCTA+AGG - chr2_1:70650853-70650872 None:intergenic 25.0%
! AACCTAATAAAAGATTGGAT+CGG - chr2_1:70651108-70651127 None:intergenic 25.0%
! AGAACAACCTAATAAAAGAT+TGG - chr2_1:70651113-70651132 None:intergenic 25.0%
! AAAAAAAAAACGAACGAAGA+GGG - chr2_1:70650168-70650187 None:intergenic 25.0%
! AAAAAAAAAAACGAACGAAG+AGG - chr2_1:70650169-70650188 None:intergenic 25.0%
! TGGAATTGTAATAGAATACT+TGG + chr2_1:70650350-70650369 Msa0221860:intron 25.0%
! GGAATTGTAATAGAATACTT+GGG + chr2_1:70650351-70650370 Msa0221860:intron 25.0%
! AGTGAATCAAAAAAAACCAA+AGG + chr2_1:70650595-70650614 Msa0221860:intron 25.0%
! TTATAGAATTTACCTCCTAA+GGG - chr2_1:70650852-70650871 None:intergenic 25.0%
! TTTATAGAATTTACCTCCTA+AGG - chr2_1:70650853-70650872 None:intergenic 25.0%
! AACCTAATAAAAGATTGGAT+CGG - chr2_1:70651108-70651127 None:intergenic 25.0%
! AGAACAACCTAATAAAAGAT+TGG - chr2_1:70651113-70651132 None:intergenic 25.0%
TCTAAAAACATATCTCTCTG+TGG + chr2_1:70649985-70650004 Msa0221860:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG + chr2_1:70650057-70650076 Msa0221860:intron 30.0%
! TTTGATTCTAGTTATTGGCA+CGG + chr2_1:70650101-70650120 Msa0221860:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG - chr2_1:70650256-70650275 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG + chr2_1:70650330-70650349 Msa0221860:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG + chr2_1:70650354-70650373 Msa0221860:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG + chr2_1:70650598-70650617 Msa0221860:intron 30.0%
TATTCGTATATCTTTACCCT+TGG - chr2_1:70650631-70650650 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr2_1:70650685-70650704 Msa0221860:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr2_1:70650686-70650705 Msa0221860:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr2_1:70650687-70650706 Msa0221860:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr2_1:70650783-70650802 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr2_1:70650784-70650803 None:intergenic 30.0%
GTATAGGAATAAACAAACCA+TGG + chr2_1:70651026-70651045 Msa0221860:CDS 30.0%
! AATCTAAGCAATCTTTTCGT+AGG + chr2_1:70651073-70651092 Msa0221860:CDS 30.0%
ACCTAATAAAAGATTGGATC+GGG - chr2_1:70651107-70651126 None:intergenic 30.0%
TCTAAAAACATATCTCTCTG+TGG + chr2_1:70649985-70650004 Msa0221860:CDS 30.0%
AGAAATCAATCGTTTATTCC+CGG + chr2_1:70650057-70650076 Msa0221860:intron 30.0%
! TTTGATTCTAGTTATTGGCA+CGG + chr2_1:70650101-70650120 Msa0221860:intron 30.0%
AAAAACTCCTAATTTCACTG+AGG - chr2_1:70650256-70650275 None:intergenic 30.0%
TAATAGAGTGAGACCATAAA+TGG + chr2_1:70650330-70650349 Msa0221860:intron 30.0%
ATTGTAATAGAATACTTGGG+CGG + chr2_1:70650354-70650373 Msa0221860:intron 30.0%
GAATCAAAAAAAACCAAAGG+AGG + chr2_1:70650598-70650617 Msa0221860:intron 30.0%
TATTCGTATATCTTTACCCT+TGG - chr2_1:70650631-70650650 None:intergenic 30.0%
! AGAGTGTTAATAAGGAATCT+AGG + chr2_1:70650685-70650704 Msa0221860:CDS 30.0%
GAGTGTTAATAAGGAATCTA+GGG + chr2_1:70650686-70650705 Msa0221860:CDS 30.0%
AGTGTTAATAAGGAATCTAG+GGG + chr2_1:70650687-70650706 Msa0221860:CDS 30.0%
ATCGTATGTTTGCAACAAAA+GGG - chr2_1:70650783-70650802 None:intergenic 30.0%
AATCGTATGTTTGCAACAAA+AGG - chr2_1:70650784-70650803 None:intergenic 30.0%
GTATAGGAATAAACAAACCA+TGG + chr2_1:70651026-70651045 Msa0221860:CDS 30.0%
! AATCTAAGCAATCTTTTCGT+AGG + chr2_1:70651073-70651092 Msa0221860:CDS 30.0%
ACCTAATAAAAGATTGGATC+GGG - chr2_1:70651107-70651126 None:intergenic 30.0%
! TGAAATTAGGAGTTTTTCCC+AGG + chr2_1:70650259-70650278 Msa0221860:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG - chr2_1:70650279-70650298 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG - chr2_1:70650280-70650299 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG + chr2_1:70650355-70650374 Msa0221860:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG - chr2_1:70650556-70650575 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG + chr2_1:70650605-70650624 Msa0221860:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG - chr2_1:70650753-70650772 None:intergenic 35.0%
! AGAAATGGGATTTTCGGTAT+AGG + chr2_1:70651010-70651029 Msa0221860:intron 35.0%
CCTAATAAAAGATTGGATCG+GGG - chr2_1:70651106-70651125 None:intergenic 35.0%
! TGAAATTAGGAGTTTTTCCC+AGG + chr2_1:70650259-70650278 Msa0221860:intron 35.0%
CTTCAATTCCATTGAAACCT+GGG - chr2_1:70650279-70650298 None:intergenic 35.0%
ACTTCAATTCCATTGAAACC+TGG - chr2_1:70650280-70650299 None:intergenic 35.0%
TTGTAATAGAATACTTGGGC+GGG + chr2_1:70650355-70650374 Msa0221860:intron 35.0%
TTCGAAGCGAAGAAGAAAAA+AGG - chr2_1:70650556-70650575 None:intergenic 35.0%
AAAAAACCAAAGGAGGTTCA+TGG + chr2_1:70650605-70650624 Msa0221860:intron 35.0%
TTTCTCAATTCCAATCGACT+AGG - chr2_1:70650753-70650772 None:intergenic 35.0%
! AGAAATGGGATTTTCGGTAT+AGG + chr2_1:70651010-70651029 Msa0221860:intron 35.0%
CCTAATAAAAGATTGGATCG+GGG - chr2_1:70651106-70651125 None:intergenic 35.0%
GATTTGGCCTCAGTGAAATT+AGG + chr2_1:70650246-70650265 Msa0221860:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG + chr2_1:70650268-70650287 Msa0221860:intron 40.0%
ACACAATACACCTAGTCGAT+TGG + chr2_1:70650740-70650759 Msa0221860:intron 40.0%
ATTGTTTGTGTGTCACCCTT+AGG + chr2_1:70650834-70650853 Msa0221860:intron 40.0%
GAGTCGCTTAGATTTATCCA+TGG - chr2_1:70651046-70651065 None:intergenic 40.0%
! CCCCGATCCAATCTTTTATT+AGG + chr2_1:70651103-70651122 Msa0221860:CDS 40.0%
GATTTGGCCTCAGTGAAATT+AGG + chr2_1:70650246-70650265 Msa0221860:intron 40.0%
! GAGTTTTTCCCAGGTTTCAA+TGG + chr2_1:70650268-70650287 Msa0221860:intron 40.0%
ACACAATACACCTAGTCGAT+TGG + chr2_1:70650740-70650759 Msa0221860:intron 40.0%
ATTGTTTGTGTGTCACCCTT+AGG + chr2_1:70650834-70650853 Msa0221860:intron 40.0%
GAGTCGCTTAGATTTATCCA+TGG - chr2_1:70651046-70651065 None:intergenic 40.0%
! CCCCGATCCAATCTTTTATT+AGG + chr2_1:70651103-70651122 Msa0221860:CDS 40.0%
! AACCATAGAGTACTTGCCAC+TGG - chr2_1:70650013-70650032 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG - chr2_1:70650079-70650098 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG + chr2_1:70650106-70650125 Msa0221860:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG + chr2_1:70650107-70650126 Msa0221860:intron 45.0%
! AACCATAGAGTACTTGCCAC+TGG - chr2_1:70650013-70650032 None:intergenic 45.0%
AAGGGGAATGTCAATGCATC+CGG - chr2_1:70650079-70650098 None:intergenic 45.0%
! TTCTAGTTATTGGCACGGCA+AGG + chr2_1:70650106-70650125 Msa0221860:intron 45.0%
TCTAGTTATTGGCACGGCAA+GGG + chr2_1:70650107-70650126 Msa0221860:intron 45.0%
!!! ATTATTATGTTTTTTTTTTT+GGG - chr2_1:70651139-70651158 None:intergenic 5.0%
!!! AATTATTATGTTTTTTTTTT+TGG - chr2_1:70651140-70651159 None:intergenic 5.0%
!!! ATTATTATGTTTTTTTTTTT+GGG - chr2_1:70651139-70651158 None:intergenic 5.0%
!!! AATTATTATGTTTTTTTTTT+TGG - chr2_1:70651140-70651159 None:intergenic 5.0%
ATATCTCTCTGTGGCACCAG+TGG + chr2_1:70649994-70650013 Msa0221860:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG + chr2_1:70650008-70650027 Msa0221860:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG + chr2_1:70650015-70650034 Msa0221860:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG - chr2_1:70650078-70650097 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG + chr2_1:70650108-70650127 Msa0221860:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG + chr2_1:70650612-70650631 Msa0221860:intron 50.0%
GTTTGTGTGTCACCCTTAGG+AGG + chr2_1:70650837-70650856 Msa0221860:intron 50.0%
ATATCTCTCTGTGGCACCAG+TGG + chr2_1:70649994-70650013 Msa0221860:CDS 50.0%
CACCAGTGGCAAGTACTCTA+TGG + chr2_1:70650008-70650027 Msa0221860:CDS 50.0%
GGCAAGTACTCTATGGTTCG+CGG + chr2_1:70650015-70650034 Msa0221860:CDS 50.0%
AGGGGAATGTCAATGCATCC+GGG - chr2_1:70650078-70650097 None:intergenic 50.0%
CTAGTTATTGGCACGGCAAG+GGG + chr2_1:70650108-70650127 Msa0221860:intron 50.0%
CAAAGGAGGTTCATGGCCAA+GGG + chr2_1:70650612-70650631 Msa0221860:intron 50.0%
GTTTGTGTGTCACCCTTAGG+AGG + chr2_1:70650837-70650856 Msa0221860:intron 50.0%
! CTATGGTTCGCGGTTCTAGC+AGG + chr2_1:70650025-70650044 Msa0221860:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG - chr2_1:70650614-70650633 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG + chr2_1:70650611-70650630 Msa0221860:intron 55.0%
! CTATGGTTCGCGGTTCTAGC+AGG + chr2_1:70650025-70650044 Msa0221860:CDS 55.0%
CCTTGGCCATGAACCTCCTT+TGG - chr2_1:70650614-70650633 None:intergenic 55.0%
CCAAAGGAGGTTCATGGCCA+AGG + chr2_1:70650611-70650630 Msa0221860:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 70649977 70651161 70649977 ID=Msa0221860;Name=Msa0221860
chr2_1 mRNA 70649977 70651161 70649977 ID=Msa0221860-mRNA-1;Parent=Msa0221860;Name=Msa0221860-mRNA-1;_AED=0.41;_eAED=0.49;_QI=0|0|0|1|0|0|3|0|91
chr2_1 exon 70649977 70650067 70649977 ID=Msa0221860-mRNA-1:exon:20271;Parent=Msa0221860-mRNA-1
chr2_1 exon 70650674 70650708 70650674 ID=Msa0221860-mRNA-1:exon:20272;Parent=Msa0221860-mRNA-1
chr2_1 exon 70651012 70651161 70651012 ID=Msa0221860-mRNA-1:exon:20273;Parent=Msa0221860-mRNA-1
chr2_1 CDS 70649977 70650067 70649977 ID=Msa0221860-mRNA-1:cds;Parent=Msa0221860-mRNA-1
chr2_1 CDS 70650674 70650708 70650674 ID=Msa0221860-mRNA-1:cds;Parent=Msa0221860-mRNA-1
chr2_1 CDS 70651012 70651161 70651012 ID=Msa0221860-mRNA-1:cds;Parent=Msa0221860-mRNA-1
Gene Sequence

>Msa0221860

ATGCGAGATCTAAAAACATATCTCTCTGTGGCACCAGTGGCAAGTACTCTATGGTTCGCGGTTCTAGCAGGTCTCTTGATAGAAATCAATCTTGTGATAAAAAGAGTGTTAATAAGGAATCTAGGGAAATGGGATTTTCGGTATAGGAATAAACAAACCATGGATAAATCTAAGCGACTCTTTCTTAAATCTAAGCAATCTTTTCGTAGGAGTTTGTCCCCGATCCAATCTTTTATTAGGTTGTTCTTTTCCCAAAAAAAAAAACATAATAATTAA

Protein sequence

>Msa0221860

MRDLKTYLSVAPVASTLWFAVLAGLLIEINLVIKRVLIRNLGKWDFRYRNKQTMDKSKRLFLKSKQSFRRSLSPIQSFIRLFFSQKKKHNN*