Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737120 | KEH26597.1 | 42.537 | 134 | 53 | 1 | 1 | 110 | 151 | 284 | 1.48e-26 | 111 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737120 | A0A072UAC5 | 42.537 | 134 | 53 | 1 | 1 | 110 | 151 | 284 | 7.05e-27 | 111 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0063890 | Msa0737120 | 0.810241 | 1.271376e-50 | -8.615850e-47 |
| Msa0101440 | Msa0737120 | 0.810331 | 1.215368e-50 | -8.615850e-47 |
| Msa0120400 | Msa0737120 | 0.805140 | 1.541532e-49 | -8.615850e-47 |
| Msa0737120 | Msa0967380 | 0.804227 | 2.389726e-49 | -8.615850e-47 |
| Msa0737120 | Msa1000250 | 0.812324 | 4.489723e-51 | -8.615850e-47 |
| Msa0737120 | Msa1121490 | 0.800134 | 1.661722e-48 | -8.615850e-47 |
| Msa0737120 | Msa1242670 | 0.811176 | 7.982011e-51 | -8.615850e-47 |
| Msa0737120 | Msa1419370 | 0.807395 | 5.162744e-50 | -8.615850e-47 |
| Msa0124060 | Msa0737120 | 0.800203 | 1.608966e-48 | -8.615850e-47 |
| Msa0139060 | Msa0737120 | 0.813761 | 2.172846e-51 | -8.615850e-47 |
| Msa0440750 | Msa0737120 | 0.801882 | 7.299698e-49 | -8.615850e-47 |
| Msa0600700 | Msa0737120 | 0.809345 | 1.980690e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0737120 | MtrunA17_Chr8g0366201 | 79.661 | 59 | 11 | 1 | 52 | 110 | 31 | 88 | 4.06e-27 | 97.1 |
| Msa0737120 | MtrunA17_Chr4g0042631 | 84.000 | 50 | 8 | 0 | 3 | 52 | 96 | 145 | 2.00e-24 | 92.0 |
| Msa0737120 | MtrunA17_Chr4g0023021 | 62.903 | 62 | 23 | 0 | 52 | 113 | 33 | 94 | 1.73e-22 | 85.5 |
| Msa0737120 | MtrunA17_Chr8g0365871 | 55.224 | 67 | 25 | 1 | 36 | 102 | 31 | 92 | 1.32e-17 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAGAAGCTATTTGGAAAT+TGG | 0.198762 | 5_1:+54079128 | Msa0737120:intron |
| AGTTACTTTGATAAATAAAT+TGG | 0.221391 | 5_1:+54079373 | Msa0737120:exon |
| TATTATAATCAGAAGCTATT+TGG | 0.227740 | 5_1:+54079121 | Msa0737120:intron |
| TTGGTAAGATGGTATGTAAA+AGG | 0.298662 | 5_1:+54079392 | Msa0737120:three_prime_UTR |
| ACAGCTGCGGACATTTCTTC+TGG | 0.305832 | 5_1:+54079011 | Msa0737120:CDS |
| AGCTATTTGGAAATTGGTTA+TGG | 0.340909 | 5_1:+54079134 | Msa0737120:CDS |
| TTGTTCTTGCCATCAACTTA+CGG | 0.354553 | 5_1:-54079221 | None:intergenic |
| TGTTCTTGCCATCAACTTAC+GGG | 0.358089 | 5_1:-54079220 | None:intergenic |
| GGTGTTGGCATTGGCGAAAA+TGG | 0.395011 | 5_1:+54079311 | Msa0737120:CDS |
| AATAAGGCATCCACTTGAAT+AGG | 0.407111 | 5_1:-54079423 | None:intergenic |
| TGATAAATAAATTGGTAAGA+TGG | 0.410540 | 5_1:+54079381 | Msa0737120:exon |
| GTGCAACGCCCGTAAGTTGA+TGG | 0.418678 | 5_1:+54079212 | Msa0737120:CDS |
| TGGTAAGATGGTATGTAAAA+GGG | 0.453244 | 5_1:+54079393 | Msa0737120:three_prime_UTR |
| AGAAACTCGGTAGCTCGATA+TGG | 0.468637 | 5_1:+54078933 | Msa0737120:CDS |
| TACCGAGTTTCTGTACTCAT+TGG | 0.471215 | 5_1:-54078922 | None:intergenic |
| GCCAATGCCAACACCAACAT+TGG | 0.474402 | 5_1:-54079303 | None:intergenic |
| CACGGGAGCAGCTAACAATA+AGG | 0.482265 | 5_1:+54079260 | Msa0737120:CDS |
| TCCAATGTTGGTGTTGGCAT+TGG | 0.485115 | 5_1:+54079302 | Msa0737120:CDS |
| TGCACCTCCAATGTTGGTGT+TGG | 0.485360 | 5_1:+54079296 | Msa0737120:CDS |
| TTACGGGCGTTGCACAGAGC+TGG | 0.507146 | 5_1:-54079204 | None:intergenic |
| AGAATGTTGTTGCAGAATCA+AGG | 0.516563 | 5_1:+54078897 | None:intergenic |
| AAGATCAAACTTGGTGTCAC+GGG | 0.530779 | 5_1:+54079243 | Msa0737120:CDS |
| GGACATTTCTTCTGGAGTGT+TGG | 0.533907 | 5_1:+54079019 | Msa0737120:CDS |
| TTTCTATCACACAATTGTCA+AGG | 0.551277 | 5_1:+54078983 | Msa0737120:CDS |
| TCTTTGTGCACCTCCAATGT+TGG | 0.556229 | 5_1:+54079290 | Msa0737120:CDS |
| GCAAGAACAAAGATCAAACT+TGG | 0.571276 | 5_1:+54079234 | Msa0737120:CDS |
| AAAGATCAAACTTGGTGTCA+CGG | 0.572250 | 5_1:+54079242 | Msa0737120:CDS |
| AATGCCAACACCAACATTGG+AGG | 0.606038 | 5_1:-54079300 | None:intergenic |
| TGCCAATGAGTACAGAAACT+CGG | 0.640910 | 5_1:+54078920 | Msa0737120:CDS |
| TGTCAAGGAGTTAACAGCTG+CGG | 0.688752 | 5_1:+54078998 | Msa0737120:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTACTTTGATAAATAAAT+TGG | + | chr5_1:54079373-54079392 | Msa0737120:exon | 15.0% |
| !!! | AACATAAAAGAAAAAGTGTA+AGG | - | chr5_1:54079054-54079073 | None:intergenic | 20.0% |
| !! | TATTATAATCAGAAGCTATT+TGG | + | chr5_1:54079121-54079140 | Msa0737120:intron | 20.0% |
| !! | TGATAAATAAATTGGTAAGA+TGG | + | chr5_1:54079381-54079400 | Msa0737120:exon | 20.0% |
| ! | ATTGTGTGATAGAAAAAAGT+GGG | - | chr5_1:54078978-54078997 | None:intergenic | 25.0% |
| ! | AATTGTGTGATAGAAAAAAG+TGG | - | chr5_1:54078979-54078998 | None:intergenic | 25.0% |
| !!! | ACACTTTTTCTTTTATGTTG+TGG | + | chr5_1:54079055-54079074 | Msa0737120:intron | 25.0% |
| TTTCTATCACACAATTGTCA+AGG | + | chr5_1:54078983-54079002 | Msa0737120:CDS | 30.0% | |
| AAAAATGCATCTCAAGTTAG+AGG | - | chr5_1:54079100-54079119 | None:intergenic | 30.0% | |
| ATCAGAAGCTATTTGGAAAT+TGG | + | chr5_1:54079128-54079147 | Msa0737120:intron | 30.0% | |
| AGCTATTTGGAAATTGGTTA+TGG | + | chr5_1:54079134-54079153 | Msa0737120:CDS | 30.0% | |
| TTGGTAAGATGGTATGTAAA+AGG | + | chr5_1:54079392-54079411 | Msa0737120:three_prime_UTR | 30.0% | |
| TGGTAAGATGGTATGTAAAA+GGG | + | chr5_1:54079393-54079412 | Msa0737120:three_prime_UTR | 30.0% | |
| GAAAAAAGTGGGAGGAAAAA+TGG | - | chr5_1:54078967-54078986 | None:intergenic | 35.0% | |
| ! | GATTTGTCTTGCGTATTTTC+TGG | + | chr5_1:54079174-54079193 | Msa0737120:CDS | 35.0% |
| TTGTTCTTGCCATCAACTTA+CGG | - | chr5_1:54079224-54079243 | None:intergenic | 35.0% | |
| GCAAGAACAAAGATCAAACT+TGG | + | chr5_1:54079234-54079253 | Msa0737120:CDS | 35.0% | |
| !! | AAAGATCAAACTTGGTGTCA+CGG | + | chr5_1:54079242-54079261 | Msa0737120:CDS | 35.0% |
| TGCCAATGAGTACAGAAACT+CGG | + | chr5_1:54078920-54078939 | Msa0737120:CDS | 40.0% | |
| TACCGAGTTTCTGTACTCAT+TGG | - | chr5_1:54078925-54078944 | None:intergenic | 40.0% | |
| GTGTGATAGAAAAAAGTGGG+AGG | - | chr5_1:54078975-54078994 | None:intergenic | 40.0% | |
| GTGGTTAGATATACGTGTGT+TGG | + | chr5_1:54079074-54079093 | Msa0737120:intron | 40.0% | |
| TGTTCTTGCCATCAACTTAC+GGG | - | chr5_1:54079223-54079242 | None:intergenic | 40.0% | |
| !! | AAGATCAAACTTGGTGTCAC+GGG | + | chr5_1:54079243-54079262 | Msa0737120:CDS | 40.0% |
| AGAAACTCGGTAGCTCGATA+TGG | + | chr5_1:54078933-54078952 | Msa0737120:CDS | 45.0% | |
| TGTCAAGGAGTTAACAGCTG+CGG | + | chr5_1:54078998-54079017 | Msa0737120:CDS | 45.0% | |
| !! | GGACATTTCTTCTGGAGTGT+TGG | + | chr5_1:54079019-54079038 | Msa0737120:CDS | 45.0% |
| TCTTTGTGCACCTCCAATGT+TGG | + | chr5_1:54079290-54079309 | Msa0737120:CDS | 45.0% | |
| AATGCCAACACCAACATTGG+AGG | - | chr5_1:54079303-54079322 | None:intergenic | 45.0% | |
| !! | TCCAATGTTGGTGTTGGCAT+TGG | + | chr5_1:54079302-54079321 | Msa0737120:CDS | 45.0% |
| ACAGCTGCGGACATTTCTTC+TGG | + | chr5_1:54079011-54079030 | Msa0737120:CDS | 50.0% | |
| CACGGGAGCAGCTAACAATA+AGG | + | chr5_1:54079260-54079279 | Msa0737120:CDS | 50.0% | |
| TGCACCTCCAATGTTGGTGT+TGG | + | chr5_1:54079296-54079315 | Msa0737120:CDS | 50.0% | |
| GCCAATGCCAACACCAACAT+TGG | - | chr5_1:54079306-54079325 | None:intergenic | 50.0% | |
| !! | GGTGTTGGCATTGGCGAAAA+TGG | + | chr5_1:54079311-54079330 | Msa0737120:CDS | 50.0% |
| ! | GTGCAACGCCCGTAAGTTGA+TGG | + | chr5_1:54079212-54079231 | Msa0737120:CDS | 55.0% |
| ! | TTACGGGCGTTGCACAGAGC+TGG | - | chr5_1:54079207-54079226 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 54078900 | 54079431 | 54078900 | ID=Msa0737120;Name=Msa0737120 |
| chr5_1 | mRNA | 54078900 | 54079431 | 54078900 | ID=Msa0737120-mRNA-1;Parent=Msa0737120;Name=Msa0737120-mRNA-1;_AED=0.34;_eAED=0.55;_QI=0|0|0.5|1|1|0.5|2|48|131 |
| chr5_1 | exon | 54078900 | 54079044 | 54078900 | ID=Msa0737120-mRNA-1:exon:15207;Parent=Msa0737120-mRNA-1 |
| chr5_1 | exon | 54079133 | 54079431 | 54079133 | ID=Msa0737120-mRNA-1:exon:15208;Parent=Msa0737120-mRNA-1 |
| chr5_1 | CDS | 54078900 | 54079044 | 54078900 | ID=Msa0737120-mRNA-1:cds;Parent=Msa0737120-mRNA-1 |
| chr5_1 | CDS | 54079133 | 54079383 | 54079133 | ID=Msa0737120-mRNA-1:cds;Parent=Msa0737120-mRNA-1 |
| chr5_1 | three_prime_UTR | 54079384 | 54079431 | 54079384 | ID=Msa0737120-mRNA-1:three_prime_utr;Parent=Msa0737120-mRNA-1 |
| Gene Sequence |
| Protein sequence |
