AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0757070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0757070 MtrunA17_Chr5g0402331 100.000 51 0 0 29 79 139 189 4.59e-31 107
Msa0757070 MtrunA17_Chr5g0402361 100.000 51 0 0 29 79 311 361 9.30e-30 107
Msa0757070 MtrunA17_Chr5g0402361 96.000 50 2 0 29 78 138 187 4.05e-27 100
Msa0757070 MtrunA17_Chr1g0161211 60.000 45 18 0 29 73 137 181 1.44e-13 62.4
Msa0757070 MtrunA17_Chr8g0391691 59.184 49 20 0 29 77 127 175 1.98e-12 59.3
Msa0757070 MtrunA17_Chr4g0044961 52.273 44 21 0 29 72 146 189 8.58e-12 57.8
Msa0757070 MtrunA17_Chr3g0111371 36.250 80 41 1 3 72 56 135 3.80e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0757070 AT1G24620.1 41.892 74 37 2 9 77 104 176 2.28e-12 59.3
Msa0757070 AT1G66400.1 53.333 45 21 0 29 73 108 152 1.26e-11 57.0
Msa0757070 AT5G37770.1 53.191 47 22 0 29 75 112 158 1.73e-11 56.6
Msa0757070 AT5G37770.2 53.191 47 22 0 29 75 112 158 1.73e-11 56.6
Msa0757070 AT2G15680.1 52.273 44 21 0 29 72 139 182 4.62e-11 55.8

Find 12 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTAGTGCTTGTAAGAAAA+TGG 0.341545 5_2:+11788270 Msa0757070:CDS
AATTGTCTCAAATATTTAAG+AGG 0.413219 5_2:+11788230 Msa0757070:CDS
TCTCAAATATTTAAGAGGTT+AGG 0.421604 5_2:+11788235 Msa0757070:CDS
TAGATTTGAATGAGTTTACA+AGG 0.501078 5_2:+11788326 Msa0757070:CDS
TGTTCTGAGCAGATGATAAG+AGG 0.530194 5_2:-11787223 None:intergenic
GTGAAAGGTGTTGATAGTGA+TGG 0.562762 5_2:+11788292 Msa0757070:CDS
TCTTTACTCAATTAGTGCTG+AGG 0.563047 5_2:+11788207 Msa0757070:intron
TTTACAAGGATGATGATGAA+TGG 0.601621 5_2:+11788340 Msa0757070:CDS
GGTGTTGATAGTGATGGAGA+TGG 0.629261 5_2:+11788298 Msa0757070:CDS
ATCATCTGCTCAGAACATTG+TGG 0.648833 5_2:+11787229 Msa0757070:CDS
GCTTGTAAGAAAATGGTGAA+AGG 0.660596 5_2:+11788277 Msa0757070:CDS
TATATTGTTCTAGCAAAACA+AGG 0.674335 5_2:+11787197 Msa0757070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTTTATGAATGATATTTT+TGG - chr5_2:11787539-11787558 None:intergenic 15.0%
!!! AACTTTATGAATGATATTTT+TGG - chr5_2:11787539-11787558 None:intergenic 15.0%
!!! TAGGTAACTTTTTATAACAT+TGG - chr5_2:11787565-11787584 None:intergenic 20.0%
!! AATTATAGATCTCAAAATGT+GGG - chr5_2:11787818-11787837 None:intergenic 20.0%
!! AAATTATAGATCTCAAAATG+TGG - chr5_2:11787819-11787838 None:intergenic 20.0%
!!! TTTTTAGTTTTGAAAACAGT+TGG + chr5_2:11787897-11787916 Msa0757070:intron 20.0%
!!! TTTTAGTTTTGAAAACAGTT+GGG + chr5_2:11787898-11787917 Msa0757070:intron 20.0%
!! AATTGTCTCAAATATTTAAG+AGG + chr5_2:11788230-11788249 Msa0757070:CDS 20.0%
!!! TAGGTAACTTTTTATAACAT+TGG - chr5_2:11787565-11787584 None:intergenic 20.0%
!! AATTATAGATCTCAAAATGT+GGG - chr5_2:11787818-11787837 None:intergenic 20.0%
!! AAATTATAGATCTCAAAATG+TGG - chr5_2:11787819-11787838 None:intergenic 20.0%
!!! TTTTTAGTTTTGAAAACAGT+TGG + chr5_2:11787897-11787916 Msa0757070:intron 20.0%
!!! TTTTAGTTTTGAAAACAGTT+GGG + chr5_2:11787898-11787917 Msa0757070:intron 20.0%
!! AATTGTCTCAAATATTTAAG+AGG + chr5_2:11788230-11788249 Msa0757070:CDS 20.0%
! TATATTGTTCTAGCAAAACA+AGG + chr5_2:11787197-11787216 Msa0757070:CDS 25.0%
! TCTAATGTATGTTCTTTGAT+GGG - chr5_2:11787345-11787364 None:intergenic 25.0%
! ATCTAATGTATGTTCTTTGA+TGG - chr5_2:11787346-11787365 None:intergenic 25.0%
!! ACTATTGGTTATCAAACTTT+AGG - chr5_2:11787584-11787603 None:intergenic 25.0%
!! TTGATAACCAATAGTTATGT+AGG + chr5_2:11787589-11787608 Msa0757070:intron 25.0%
! CAATAAACCTACATAACTAT+TGG - chr5_2:11787599-11787618 None:intergenic 25.0%
! CTGTTATGATAAAAGGAAAA+AGG - chr5_2:11787639-11787658 None:intergenic 25.0%
! AATCTCCTTTGATATACAAT+TGG - chr5_2:11787665-11787684 None:intergenic 25.0%
! TTGTATATCAAAGGAGATTA+TGG + chr5_2:11787666-11787685 Msa0757070:intron 25.0%
!! ATCTATAATTTGCATCTTGT+GGG + chr5_2:11787828-11787847 Msa0757070:intron 25.0%
! GTTATCTGTCAAAAGTAAAA+GGG - chr5_2:11787986-11788005 None:intergenic 25.0%
!!! CTTTTACTTTTGACAGATAA+CGG + chr5_2:11787985-11788004 Msa0757070:intron 25.0%
!!! TTTTACTTTTGACAGATAAC+GGG + chr5_2:11787986-11788005 Msa0757070:intron 25.0%
!!! TTTTGTTTCTTTGAATAGCT+CGG + chr5_2:11788010-11788029 Msa0757070:intron 25.0%
! AGGTATGTTCAAACTTTAAA+TGG - chr5_2:11788080-11788099 None:intergenic 25.0%
!!! ACAATGTTTTGAGTTGTTAT+TGG - chr5_2:11788142-11788161 None:intergenic 25.0%
! TCTCAAATATTTAAGAGGTT+AGG + chr5_2:11788235-11788254 Msa0757070:CDS 25.0%
! TAGATTTGAATGAGTTTACA+AGG + chr5_2:11788326-11788345 Msa0757070:CDS 25.0%
! TATATTGTTCTAGCAAAACA+AGG + chr5_2:11787197-11787216 Msa0757070:CDS 25.0%
! TCTAATGTATGTTCTTTGAT+GGG - chr5_2:11787345-11787364 None:intergenic 25.0%
! ATCTAATGTATGTTCTTTGA+TGG - chr5_2:11787346-11787365 None:intergenic 25.0%
!! ACTATTGGTTATCAAACTTT+AGG - chr5_2:11787584-11787603 None:intergenic 25.0%
!! TTGATAACCAATAGTTATGT+AGG + chr5_2:11787589-11787608 Msa0757070:intron 25.0%
! CAATAAACCTACATAACTAT+TGG - chr5_2:11787599-11787618 None:intergenic 25.0%
! CTGTTATGATAAAAGGAAAA+AGG - chr5_2:11787639-11787658 None:intergenic 25.0%
! AATCTCCTTTGATATACAAT+TGG - chr5_2:11787665-11787684 None:intergenic 25.0%
! TTGTATATCAAAGGAGATTA+TGG + chr5_2:11787666-11787685 Msa0757070:intron 25.0%
!! ATCTATAATTTGCATCTTGT+GGG + chr5_2:11787828-11787847 Msa0757070:intron 25.0%
! GTTATCTGTCAAAAGTAAAA+GGG - chr5_2:11787986-11788005 None:intergenic 25.0%
!!! CTTTTACTTTTGACAGATAA+CGG + chr5_2:11787985-11788004 Msa0757070:intron 25.0%
!!! TTTTACTTTTGACAGATAAC+GGG + chr5_2:11787986-11788005 Msa0757070:intron 25.0%
!!! TTTTGTTTCTTTGAATAGCT+CGG + chr5_2:11788010-11788029 Msa0757070:intron 25.0%
! AGGTATGTTCAAACTTTAAA+TGG - chr5_2:11788080-11788099 None:intergenic 25.0%
!!! ACAATGTTTTGAGTTGTTAT+TGG - chr5_2:11788142-11788161 None:intergenic 25.0%
! TCTCAAATATTTAAGAGGTT+AGG + chr5_2:11788235-11788254 Msa0757070:CDS 25.0%
! TAGATTTGAATGAGTTTACA+AGG + chr5_2:11788326-11788345 Msa0757070:CDS 25.0%
ACATTAGATAGCAGAAAACT+AGG + chr5_2:11787357-11787376 Msa0757070:intron 30.0%
TTGGCATCTGTTATGATAAA+AGG - chr5_2:11787646-11787665 None:intergenic 30.0%
AGATGCCAATTGTATATCAA+AGG + chr5_2:11787657-11787676 Msa0757070:intron 30.0%
AAAATGTGGGAATCTATTTC+AGG - chr5_2:11787805-11787824 None:intergenic 30.0%
! GATCTATAATTTGCATCTTG+TGG + chr5_2:11787827-11787846 Msa0757070:intron 30.0%
ATAGAACAAACTCAATTGCA+AGG - chr5_2:11787963-11787982 None:intergenic 30.0%
CGTTATCTGTCAAAAGTAAA+AGG - chr5_2:11787987-11788006 None:intergenic 30.0%
! TTTACAAGGATGATGATGAA+TGG + chr5_2:11788340-11788359 Msa0757070:CDS 30.0%
ACATTAGATAGCAGAAAACT+AGG + chr5_2:11787357-11787376 Msa0757070:intron 30.0%
TTGGCATCTGTTATGATAAA+AGG - chr5_2:11787646-11787665 None:intergenic 30.0%
AGATGCCAATTGTATATCAA+AGG + chr5_2:11787657-11787676 Msa0757070:intron 30.0%
AAAATGTGGGAATCTATTTC+AGG - chr5_2:11787805-11787824 None:intergenic 30.0%
! GATCTATAATTTGCATCTTG+TGG + chr5_2:11787827-11787846 Msa0757070:intron 30.0%
ATAGAACAAACTCAATTGCA+AGG - chr5_2:11787963-11787982 None:intergenic 30.0%
CGTTATCTGTCAAAAGTAAA+AGG - chr5_2:11787987-11788006 None:intergenic 30.0%
! TTTACAAGGATGATGATGAA+TGG + chr5_2:11788340-11788359 Msa0757070:CDS 30.0%
! GTACTTGTTGCATTGTCTTT+AGG + chr5_2:11787422-11787441 Msa0757070:intron 35.0%
! GCATTGTCTTTAGGAATTTC+AGG + chr5_2:11787431-11787450 Msa0757070:intron 35.0%
TAAGTTCTTGGAATTGCGTA+AGG - chr5_2:11787459-11787478 None:intergenic 35.0%
TTACGCAATTCCAAGAACTT+AGG + chr5_2:11787458-11787477 Msa0757070:intron 35.0%
TCAGAGACTTTATGTAGCTA+AGG + chr5_2:11787485-11787504 Msa0757070:intron 35.0%
CTGTTGTTGAGTATGAAGAT+CGG + chr5_2:11787751-11787770 Msa0757070:intron 35.0%
TGTTGTTGAGTATGAAGATC+GGG + chr5_2:11787752-11787771 Msa0757070:intron 35.0%
TACTAAAGCCTCTATACAGT+TGG + chr5_2:11787921-11787940 Msa0757070:intron 35.0%
ACTAAAGCCTCTATACAGTT+GGG + chr5_2:11787922-11787941 Msa0757070:intron 35.0%
TATTGTACCCAACTGTATAG+AGG - chr5_2:11787932-11787951 None:intergenic 35.0%
TCTATACAGTTGGGTACAAT+AGG + chr5_2:11787931-11787950 Msa0757070:intron 35.0%
AAAGTTTGAACATACCTGTG+AGG + chr5_2:11788083-11788102 Msa0757070:intron 35.0%
AAGTTTGAACATACCTGTGA+GGG + chr5_2:11788084-11788103 Msa0757070:intron 35.0%
!! TTTAGGTTGTGTAAGGTCTA+CGG + chr5_2:11788108-11788127 Msa0757070:intron 35.0%
TCTTTACTCAATTAGTGCTG+AGG + chr5_2:11788207-11788226 Msa0757070:intron 35.0%
! CCATTTTCTTACAAGCACTA+AGG - chr5_2:11788273-11788292 None:intergenic 35.0%
CCTTAGTGCTTGTAAGAAAA+TGG + chr5_2:11788270-11788289 Msa0757070:CDS 35.0%
GCTTGTAAGAAAATGGTGAA+AGG + chr5_2:11788277-11788296 Msa0757070:CDS 35.0%
! GTACTTGTTGCATTGTCTTT+AGG + chr5_2:11787422-11787441 Msa0757070:intron 35.0%
! GCATTGTCTTTAGGAATTTC+AGG + chr5_2:11787431-11787450 Msa0757070:intron 35.0%
TAAGTTCTTGGAATTGCGTA+AGG - chr5_2:11787459-11787478 None:intergenic 35.0%
TTACGCAATTCCAAGAACTT+AGG + chr5_2:11787458-11787477 Msa0757070:intron 35.0%
TCAGAGACTTTATGTAGCTA+AGG + chr5_2:11787485-11787504 Msa0757070:intron 35.0%
CTGTTGTTGAGTATGAAGAT+CGG + chr5_2:11787751-11787770 Msa0757070:intron 35.0%
TGTTGTTGAGTATGAAGATC+GGG + chr5_2:11787752-11787771 Msa0757070:intron 35.0%
TACTAAAGCCTCTATACAGT+TGG + chr5_2:11787921-11787940 Msa0757070:intron 35.0%
ACTAAAGCCTCTATACAGTT+GGG + chr5_2:11787922-11787941 Msa0757070:intron 35.0%
TATTGTACCCAACTGTATAG+AGG - chr5_2:11787932-11787951 None:intergenic 35.0%
TCTATACAGTTGGGTACAAT+AGG + chr5_2:11787931-11787950 Msa0757070:intron 35.0%
AAAGTTTGAACATACCTGTG+AGG + chr5_2:11788083-11788102 Msa0757070:intron 35.0%
AAGTTTGAACATACCTGTGA+GGG + chr5_2:11788084-11788103 Msa0757070:intron 35.0%
!! TTTAGGTTGTGTAAGGTCTA+CGG + chr5_2:11788108-11788127 Msa0757070:intron 35.0%
TCTTTACTCAATTAGTGCTG+AGG + chr5_2:11788207-11788226 Msa0757070:intron 35.0%
! CCATTTTCTTACAAGCACTA+AGG - chr5_2:11788273-11788292 None:intergenic 35.0%
CCTTAGTGCTTGTAAGAAAA+TGG + chr5_2:11788270-11788289 Msa0757070:CDS 35.0%
GCTTGTAAGAAAATGGTGAA+AGG + chr5_2:11788277-11788296 Msa0757070:CDS 35.0%
! TGTTCTGAGCAGATGATAAG+AGG - chr5_2:11787226-11787245 None:intergenic 40.0%
ATCATCTGCTCAGAACATTG+TGG + chr5_2:11787229-11787248 Msa0757070:CDS 40.0%
!! CTCTGAGATTCCTAAGTTCT+TGG - chr5_2:11787471-11787490 None:intergenic 40.0%
GTTGTTGAGTATGAAGATCG+GGG + chr5_2:11787753-11787772 Msa0757070:intron 40.0%
AACATACCTGTGAGGGATTT+AGG + chr5_2:11788091-11788110 Msa0757070:intron 40.0%
!! TGAGGGATTTAGGTTGTGTA+AGG + chr5_2:11788101-11788120 Msa0757070:intron 40.0%
!! GTGAAAGGTGTTGATAGTGA+TGG + chr5_2:11788292-11788311 Msa0757070:CDS 40.0%
! TGTTCTGAGCAGATGATAAG+AGG - chr5_2:11787226-11787245 None:intergenic 40.0%
ATCATCTGCTCAGAACATTG+TGG + chr5_2:11787229-11787248 Msa0757070:CDS 40.0%
!! CTCTGAGATTCCTAAGTTCT+TGG - chr5_2:11787471-11787490 None:intergenic 40.0%
GTTGTTGAGTATGAAGATCG+GGG + chr5_2:11787753-11787772 Msa0757070:intron 40.0%
AACATACCTGTGAGGGATTT+AGG + chr5_2:11788091-11788110 Msa0757070:intron 40.0%
!! TGAGGGATTTAGGTTGTGTA+AGG + chr5_2:11788101-11788120 Msa0757070:intron 40.0%
!! GTGAAAGGTGTTGATAGTGA+TGG + chr5_2:11788292-11788311 Msa0757070:CDS 40.0%
! TTGCCCAGTGGTATTGAGAA+AGG + chr5_2:11787855-11787874 Msa0757070:intron 45.0%
! TGCCCAGTGGTATTGAGAAA+GGG + chr5_2:11787856-11787875 Msa0757070:intron 45.0%
CACCCTTTCTCAATACCACT+GGG - chr5_2:11787861-11787880 None:intergenic 45.0%
TCACCCTTTCTCAATACCAC+TGG - chr5_2:11787862-11787881 None:intergenic 45.0%
ACACAACCTAAATCCCTCAC+AGG - chr5_2:11788100-11788119 None:intergenic 45.0%
!! GGTGTTGATAGTGATGGAGA+TGG + chr5_2:11788298-11788317 Msa0757070:CDS 45.0%
! TTGCCCAGTGGTATTGAGAA+AGG + chr5_2:11787855-11787874 Msa0757070:intron 45.0%
! TGCCCAGTGGTATTGAGAAA+GGG + chr5_2:11787856-11787875 Msa0757070:intron 45.0%
CACCCTTTCTCAATACCACT+GGG - chr5_2:11787861-11787880 None:intergenic 45.0%
TCACCCTTTCTCAATACCAC+TGG - chr5_2:11787862-11787881 None:intergenic 45.0%
ACACAACCTAAATCCCTCAC+AGG - chr5_2:11788100-11788119 None:intergenic 45.0%
!! GGTGTTGATAGTGATGGAGA+TGG + chr5_2:11788298-11788317 Msa0757070:CDS 45.0%
! CTTGTGGGTCTTTTGCCCAG+TGG + chr5_2:11787843-11787862 Msa0757070:intron 55.0%
! CTTGTGGGTCTTTTGCCCAG+TGG + chr5_2:11787843-11787862 Msa0757070:intron 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 11787191 11788378 11787191 ID=Msa0757070;Name=Msa0757070
chr5_2 mRNA 11787191 11788378 11787191 ID=Msa0757070-mRNA-1;Parent=Msa0757070;Name=Msa0757070-mRNA-1;_AED=0.57;_eAED=0.57;_QI=0|0|0|0.5|1|1|2|0|79
chr5_2 exon 11787191 11787273 11787191 ID=Msa0757070-mRNA-1:exon:5296;Parent=Msa0757070-mRNA-1
chr5_2 exon 11788222 11788378 11788222 ID=Msa0757070-mRNA-1:exon:5297;Parent=Msa0757070-mRNA-1
chr5_2 CDS 11787191 11787273 11787191 ID=Msa0757070-mRNA-1:cds;Parent=Msa0757070-mRNA-1
chr5_2 CDS 11788222 11788378 11788222 ID=Msa0757070-mRNA-1:cds;Parent=Msa0757070-mRNA-1
Gene Sequence

>Msa0757070

ATGAAATATATTGTTCTAGCAAAACAAGGTGACCTCTTATCATCTGCTCAGAACATTGTGGAAGTCTACATGAGTGTTGAAGATGCTGAGGAATTGTCTCAAATATTTAAGAGGTTAGGAGAGAGTTGTAGCCTTAGTGCTTGTAAGAAAATGGTGAAAGGTGTTGATAGTGATGGAGATGGTCTTATAGATTTGAATGAGTTTACAAGGATGATGATGAATGGCAAGAAATGTGCCTAA

Protein sequence

>Msa0757070

MKYIVLAKQGDLLSSAQNIVEVYMSVEDAEELSQIFKRLGESCSLSACKKMVKGVDSDGDGLIDLNEFTRMMMNGKKCA*