AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0757050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0757050 MtrunA17_Chr5g0402361 94.624 186 10 0 1 186 1 186 4.02e-123 352
Msa0757050 MtrunA17_Chr5g0402361 90.857 175 15 1 15 188 187 361 1.47e-108 315
Msa0757050 MtrunA17_Chr5g0402331 91.005 189 16 1 1 188 1 189 6.68e-121 339
Msa0757050 MtrunA17_Chr4g0044961 46.316 190 92 3 1 181 1 189 3.97e-48 155
Msa0757050 MtrunA17_Chr5g0402351 88.235 85 10 0 32 116 7 91 3.40e-46 147
Msa0757050 MtrunA17_Chr1g0161211 39.888 178 96 5 8 182 12 181 3.04e-34 120
Msa0757050 MtrunA17_Chr3g0111371 41.176 136 77 1 46 181 3 135 2.47e-32 113
Msa0757050 MtrunA17_Chr8g0391691 44.681 141 75 2 48 186 36 175 6.90e-31 111
Msa0757050 MtrunA17_Chr5g0434951 41.481 135 79 0 47 181 3 137 7.28e-30 107
Msa0757050 MtrunA17_Chr8g0392951 38.849 139 85 0 47 185 23 161 6.96e-29 105
Msa0757050 MtrunA17_Chr1g0169581 36.957 138 84 1 47 181 3 140 1.68e-26 98.6
Msa0757050 MtrunA17_Chr7g0245911 37.989 179 91 7 20 185 23 194 2.50e-26 99.8
Msa0757050 MtrunA17_Chr6g0482731 36.765 136 85 1 47 181 25 160 3.17e-26 98.6
Msa0757050 MtrunA17_Chr8g0351681 39.189 148 81 3 48 186 49 196 6.79e-26 98.6
Msa0757050 MtrunA17_Chr4g0033601 36.879 141 86 2 47 184 4 144 1.34e-23 91.3
Msa0757050 MtrunA17_Chr1g0169621 34.756 164 77 3 47 181 3 165 5.91e-23 90.1
Msa0757050 MtrunA17_Chr7g0256171 39.583 144 84 2 48 188 5 148 8.78e-23 89.4
Msa0757050 MtrunA17_Chr7g0225921 35.252 139 89 1 45 182 3 141 3.16e-22 87.4
Msa0757050 MtrunA17_Chr1g0188171 36.957 138 80 2 50 183 14 148 4.02e-22 87.4
Msa0757050 MtrunA17_Chr2g0329371 37.762 143 84 2 47 184 63 205 1.07e-21 87.8
Msa0757050 MtrunA17_Chr6g0459231 34.351 131 86 0 52 182 12 142 1.86e-21 85.5
Msa0757050 MtrunA17_Chr3g0116141 34.091 132 87 0 50 181 7 138 1.87e-21 85.5
Msa0757050 MtrunA17_Chr3g0116151 34.091 132 87 0 50 181 8 139 1.92e-21 85.5
Msa0757050 MtrunA17_Chr7g0256701 35.714 140 84 3 48 181 73 212 2.52e-21 87.0
Msa0757050 MtrunA17_Chr3g0093271 32.258 186 113 4 1 182 22 198 3.74e-21 86.3
Msa0757050 MtrunA17_Chr3g0121191 36.429 140 78 2 48 186 93 222 5.03e-21 86.7
Msa0757050 MtrunA17_Chr1g0185001 32.877 146 95 1 40 182 1 146 1.54e-20 83.2
Msa0757050 MtrunA17_Chr1g0166281 35.971 139 82 2 48 182 12 147 2.20e-20 82.8
Msa0757050 MtrunA17_Chr7g0254731 35.252 139 83 2 48 182 12 147 1.70e-19 80.5
Msa0757050 MtrunA17_Chr4g0057661 32.836 134 90 0 48 181 6 139 5.61e-19 79.0
Msa0757050 MtrunA17_Chr5g0440931 35.252 139 83 2 48 182 12 147 6.76e-19 79.0
Msa0757050 MtrunA17_Chr6g0460211 35.252 139 83 2 48 182 12 147 7.28e-19 79.0
Msa0757050 MtrunA17_Chr8g0373691 30.597 134 92 1 49 181 54 187 9.74e-19 79.7
Msa0757050 MtrunA17_Chr8g0373691 42.188 64 37 0 118 181 51 114 2.44e-11 59.7
Msa0757050 MtrunA17_Chr4g0057641 32.836 134 90 0 48 181 6 139 1.27e-18 78.2
Msa0757050 MtrunA17_Chr4g0064231 35.443 158 83 3 46 184 62 219 4.73e-18 78.6
Msa0757050 MtrunA17_Chr4g0057651 33.088 136 90 1 48 183 6 140 9.48e-18 75.9
Msa0757050 MtrunA17_Chr7g0274101 36.364 132 83 1 53 183 17 148 4.69e-17 74.3
Msa0757050 MtrunA17_Chr7g0245231 34.507 142 91 2 43 183 25 165 8.45e-17 73.9
Msa0757050 MtrunA17_Chr6g0450921 32.903 155 101 3 30 183 12 164 1.40e-16 73.6
Msa0757050 MtrunA17_Chr4g0062221 27.536 138 97 1 47 181 10 147 4.54e-16 72.0
Msa0757050 MtrunA17_Chr8g0366401 28.261 138 95 1 48 181 17 154 4.89e-16 72.0
Msa0757050 MtrunA17_Chr8g0368681 28.859 149 98 4 40 181 1 148 1.31e-15 70.9
Msa0757050 MtrunA17_Chr3g0125311 30.719 153 83 5 48 182 13 160 5.51e-15 69.3
Msa0757050 MtrunA17_Chr7g0267751 32.847 137 88 3 49 184 441 574 5.56e-15 72.4
Msa0757050 MtrunA17_Chr1g0170281 34.307 137 86 3 49 184 424 557 9.23e-15 71.6
Msa0757050 MtrunA17_Chr1g0176751 33.333 138 86 4 49 184 430 563 1.18e-14 71.2
Msa0757050 MtrunA17_Chr5g0441691 31.618 136 92 1 49 184 374 508 1.51e-14 70.9
Msa0757050 MtrunA17_Chr5g0396261 35.714 98 58 2 87 180 16 112 1.79e-14 66.6
Msa0757050 MtrunA17_Chr1g0157131 31.159 138 90 2 49 184 380 514 2.02e-14 70.5
Msa0757050 MtrunA17_Chr5g0448721 31.618 136 91 1 49 184 402 535 5.35e-14 69.3
Msa0757050 MtrunA17_Chr0c02g0489761 31.429 140 95 1 49 188 384 522 6.83e-14 68.9
Msa0757050 MtrunA17_Chr4g0068501 29.861 144 96 3 43 181 11 154 7.04e-14 66.2
Msa0757050 MtrunA17_Chr1g0160151 31.293 147 84 4 47 182 21 161 7.53e-14 66.2
Msa0757050 MtrunA17_Chr5g0444171 30.882 136 93 1 49 184 398 532 7.76e-14 68.9
Msa0757050 MtrunA17_Chr1g0200601 32.353 136 90 1 49 184 336 469 2.06e-13 67.8
Msa0757050 MtrunA17_Chr4g0074811 29.710 138 92 2 49 184 397 531 2.42e-13 67.4
Msa0757050 MtrunA17_Chr5g0397101 30.435 138 91 2 49 184 372 506 2.60e-13 67.4
Msa0757050 MtrunA17_Chr1g0173541 33.333 132 88 0 53 184 362 493 3.11e-13 67.0
Msa0757050 MtrunA17_Chr8g0385341 33.088 136 89 1 49 184 336 469 4.57e-13 66.6
Msa0757050 MtrunA17_Chr8g0387671 30.435 138 91 2 49 184 372 506 8.08e-13 65.9
Msa0757050 MtrunA17_Chr7g0257751 31.818 132 90 0 53 184 368 499 8.32e-13 65.9
Msa0757050 MtrunA17_Chr7g0242561 28.986 138 98 0 47 184 360 497 1.03e-12 65.5
Msa0757050 MtrunA17_Chr5g0405421 31.387 137 79 4 53 181 411 540 3.66e-12 63.9
Msa0757050 MtrunA17_Chr7g0245371 28.108 185 91 7 18 181 7 170 8.40e-12 60.8
Msa0757050 MtrunA17_Chr4g0059951 28.485 165 115 3 22 184 351 514 1.28e-11 62.4
Msa0757050 MtrunA17_Chr3g0098951 29.167 144 96 2 49 186 389 532 1.69e-11 62.0
Msa0757050 MtrunA17_Chr4g0074791 33.088 136 80 2 49 184 401 525 1.82e-11 62.0
Msa0757050 MtrunA17_Chr3g0131181 31.618 136 91 1 49 184 335 468 2.38e-11 61.6
Msa0757050 MtrunA17_Chr4g0042491 43.038 79 42 1 103 181 100 175 6.91e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0757050 AT2G15680.1 50.360 139 68 1 43 181 45 182 9.63e-45 147
Msa0757050 AT1G24620.1 45.390 141 74 2 48 186 37 176 1.32e-33 118
Msa0757050 AT5G37770.1 41.958 143 78 1 47 184 16 158 1.82e-33 117
Msa0757050 AT5G37770.2 41.958 143 78 1 47 184 16 158 1.82e-33 117
Msa0757050 AT1G66400.1 40.288 139 80 1 47 182 14 152 4.22e-31 111
Msa0757050 AT5G17470.1 43.885 139 74 3 52 186 6 144 2.69e-30 108
Msa0757050 AT1G18210.2 36.709 158 95 2 24 181 1 153 4.48e-30 108
Msa0757050 AT1G18210.1 36.709 158 95 2 24 181 1 153 4.48e-30 108
Msa0757050 AT3G03400.1 43.200 125 66 2 52 172 9 132 2.20e-28 103
Msa0757050 AT5G42380.1 32.597 181 116 2 3 181 8 184 2.77e-28 104
Msa0757050 AT2G36180.1 42.958 142 76 3 49 186 1 141 1.35e-27 101
Msa0757050 AT1G73630.1 37.241 145 87 2 38 181 9 150 1.41e-26 99.8
Msa0757050 AT2G43290.1 37.241 145 83 2 48 184 65 209 3.59e-24 94.7
Msa0757050 AT3G59440.1 36.691 139 86 1 48 184 51 189 3.73e-24 94.4
Msa0757050 AT3G50770.1 32.759 174 102 3 14 181 39 203 8.23e-24 93.6
Msa0757050 AT1G76650.1 31.638 177 111 1 5 181 10 176 2.19e-23 91.7
Msa0757050 AT1G76650.3 31.638 177 111 1 5 181 10 176 2.19e-23 91.7
Msa0757050 AT4G14640.1 37.143 140 81 2 48 183 13 149 1.04e-22 89.4
Msa0757050 AT3G50360.1 36.957 138 86 1 45 181 24 161 1.23e-21 87.0
Msa0757050 AT1G05990.1 38.298 141 83 2 48 184 5 145 1.57e-21 86.3
Msa0757050 AT3G22930.1 37.324 142 81 3 48 184 35 173 3.46e-21 85.9
Msa0757050 AT3G07490.1 36.429 140 86 2 48 184 5 144 1.38e-20 84.0
Msa0757050 AT4G14640.2 36.508 126 73 2 62 183 9 131 1.50e-20 83.2
Msa0757050 AT1G76640.1 34.641 153 96 2 33 181 6 158 3.40e-20 83.2
Msa0757050 AT4G03290.1 35.417 144 87 2 48 185 5 148 1.28e-19 81.3
Msa0757050 AT2G27030.3 34.965 143 86 2 48 186 12 151 5.09e-19 80.5
Msa0757050 AT5G21274.1 35.971 139 82 2 48 182 12 147 5.09e-19 79.7
Msa0757050 AT1G66410.1 35.252 139 83 2 48 182 12 147 5.54e-19 79.7
Msa0757050 AT5G37780.1 35.252 139 83 2 48 182 12 147 5.54e-19 79.7
Msa0757050 AT3G56800.1 35.971 139 82 2 48 182 12 147 6.57e-19 79.3
Msa0757050 AT2G41110.1 35.971 139 82 2 48 182 12 147 6.57e-19 79.3
Msa0757050 AT2G27030.1 35.971 139 82 2 48 182 12 147 6.57e-19 79.3
Msa0757050 AT3G43810.2 34.694 147 87 3 48 188 12 155 8.29e-19 80.9
Msa0757050 AT3G43810.1 35.252 139 83 2 48 182 12 147 1.00e-18 79.0
Msa0757050 AT4G12860.1 38.028 142 85 2 46 184 3 144 1.10e-18 79.0
Msa0757050 AT2G41410.1 28.889 180 105 6 10 182 45 208 1.61e-18 80.1
Msa0757050 AT3G10190.1 31.818 154 97 3 36 181 52 205 1.13e-17 77.8
Msa0757050 AT1G76650.2 29.379 177 105 2 5 181 10 166 2.07e-17 75.9
Msa0757050 AT3G03410.1 35.338 133 78 1 50 182 4 128 2.88e-17 74.7
Msa0757050 AT1G32250.1 28.767 146 89 3 47 181 15 156 5.07e-17 75.1
Msa0757050 AT5G04870.1 35.507 138 83 4 49 184 456 589 2.75e-16 76.6
Msa0757050 AT1G18530.1 31.884 138 89 3 48 181 7 143 3.36e-16 72.4
Msa0757050 AT4G04695.1 34.559 136 88 1 49 184 337 471 4.18e-16 75.9
Msa0757050 AT2G41110.2 33.113 151 82 3 48 182 12 159 5.58e-16 72.0
Msa0757050 AT4G37010.2 31.429 140 91 2 45 181 28 165 6.26e-16 72.0
Msa0757050 AT4G37010.1 31.429 140 91 2 45 181 24 161 6.33e-16 72.0
Msa0757050 AT5G37780.4 34.400 125 75 2 62 182 13 134 7.17e-16 71.2
Msa0757050 AT5G44460.1 34.320 169 91 6 26 181 13 174 8.32e-16 72.0
Msa0757050 AT1G66410.2 34.400 125 75 2 62 182 36 157 9.53e-16 71.2
Msa0757050 AT5G37780.3 34.400 125 75 2 62 182 52 173 1.11e-15 71.6
Msa0757050 AT2G17290.1 34.559 136 87 1 49 184 391 524 1.17e-15 74.7
Msa0757050 AT2G17290.2 34.559 136 87 1 49 184 391 524 1.17e-15 74.7
Msa0757050 AT5G23580.1 33.835 133 86 1 49 181 328 458 1.36e-15 74.3
Msa0757050 AT5G37780.2 34.400 125 75 2 62 182 41 162 1.51e-15 70.9
Msa0757050 AT5G19360.1 33.088 136 90 1 49 184 374 508 2.47e-15 73.6
Msa0757050 AT5G12180.1 33.088 136 90 1 49 184 379 513 2.55e-15 73.6
Msa0757050 AT2G41100.7 32.692 156 86 4 48 185 66 220 2.58e-15 72.4
Msa0757050 AT2G41100.6 32.692 156 86 4 48 185 66 220 2.58e-15 72.4
Msa0757050 AT2G41100.3 32.692 156 86 4 48 185 66 220 2.58e-15 72.4
Msa0757050 AT1G66410.4 41.053 95 49 2 92 182 20 111 2.83e-15 68.9
Msa0757050 AT1G66410.3 41.053 95 49 2 92 182 20 111 2.83e-15 68.9
Msa0757050 AT2G41100.4 32.692 156 86 4 48 185 101 255 3.61e-15 72.4
Msa0757050 AT2G41100.4 31.126 151 87 3 48 181 12 162 8.51e-13 65.9
Msa0757050 AT2G41100.1 32.692 156 86 4 48 185 101 255 3.61e-15 72.4
Msa0757050 AT2G41100.1 31.126 151 87 3 48 181 12 162 8.51e-13 65.9
Msa0757050 AT3G10660.2 34.307 137 86 3 49 184 492 625 4.31e-15 73.2
Msa0757050 AT3G10660.1 34.307 137 86 3 49 184 492 625 4.31e-15 73.2
Msa0757050 AT3G43810.4 41.053 95 49 2 92 182 20 111 4.56e-15 68.6
Msa0757050 AT3G43810.3 41.053 95 49 2 92 182 20 111 4.56e-15 68.6
Msa0757050 AT4G04720.2 33.088 136 90 1 49 184 385 519 8.15e-15 72.0
Msa0757050 AT4G04720.1 33.088 136 90 1 49 184 385 519 8.15e-15 72.0
Msa0757050 AT4G38230.1 34.559 136 87 1 49 184 186 319 1.07e-14 71.2
Msa0757050 AT3G03000.1 30.714 140 93 3 48 184 20 158 1.23e-14 68.6
Msa0757050 AT4G38230.4 34.559 136 87 1 49 184 330 463 1.43e-14 71.2
Msa0757050 AT4G38230.3 34.559 136 87 1 49 184 330 463 1.43e-14 71.2
Msa0757050 AT4G38230.2 34.559 136 87 1 49 184 360 493 1.49e-14 71.2
Msa0757050 AT4G09570.1 34.559 136 87 2 49 184 331 464 1.89e-14 71.2
Msa0757050 AT5G12480.2 33.577 137 90 1 49 184 271 407 2.24e-14 70.9
Msa0757050 AT3G25600.1 28.986 138 93 3 48 181 12 148 2.33e-14 67.8
Msa0757050 AT5G12480.1 33.577 137 90 1 49 184 365 501 3.17e-14 70.5
Msa0757050 AT4G04700.1 33.835 133 87 1 49 181 337 468 3.19e-14 70.5
Msa0757050 AT1G35670.1 33.824 136 88 2 49 184 332 465 3.93e-14 70.1
Msa0757050 AT2G41090.1 31.852 135 90 1 48 182 12 144 5.71e-14 67.4
Msa0757050 AT3G51850.1 28.659 164 116 1 22 184 332 495 5.72e-14 69.7
Msa0757050 AT1G21550.1 31.293 147 90 4 47 183 9 154 6.37e-14 66.6
Msa0757050 AT4G04710.2 33.088 136 90 1 49 184 338 472 6.53e-14 69.3
Msa0757050 AT4G04710.1 33.088 136 90 1 49 184 351 485 6.86e-14 69.3
Msa0757050 AT4G04710.3 33.088 136 90 1 49 184 324 458 7.97e-14 69.3
Msa0757050 AT2G38910.1 34.559 136 85 3 49 183 440 572 8.06e-14 69.3
Msa0757050 AT4G35310.2 32.353 136 90 1 49 184 403 536 9.36e-14 68.9
Msa0757050 AT4G35310.1 32.353 136 90 1 49 184 403 536 9.36e-14 68.9
Msa0757050 AT2G17890.1 30.625 160 94 4 29 181 400 549 1.04e-13 68.9
Msa0757050 AT4G23650.1 31.884 138 89 2 49 184 384 518 1.15e-13 68.9
Msa0757050 AT4G04740.4 30.435 138 91 2 49 184 374 508 1.99e-13 68.2
Msa0757050 AT4G04740.6 30.435 138 91 2 49 184 374 508 1.99e-13 68.2
Msa0757050 AT4G04740.5 30.435 138 91 2 49 184 374 508 1.99e-13 68.2
Msa0757050 AT4G04740.3 30.435 138 91 2 49 184 374 508 1.99e-13 68.2
Msa0757050 AT4G04740.1 30.435 138 91 2 49 184 374 508 1.99e-13 68.2
Msa0757050 AT4G04740.7 30.435 138 91 2 49 184 374 508 1.99e-13 68.2
Msa0757050 AT4G36070.1 33.566 143 80 5 47 181 377 512 2.18e-13 68.2
Msa0757050 AT4G36070.2 33.566 143 80 5 47 181 377 512 2.95e-13 67.8
Msa0757050 AT1G50700.1 31.618 136 92 1 49 184 379 513 6.25e-13 66.6
Msa0757050 AT3G57530.1 33.083 133 86 2 53 184 373 503 8.73e-13 66.2
Msa0757050 AT4G20780.1 29.379 177 97 4 26 181 14 183 1.21e-12 63.9
Msa0757050 AT4G04710.4 32.609 138 90 2 49 184 351 487 1.56e-12 65.5
Msa0757050 AT5G66210.1 32.353 136 79 4 53 181 374 503 1.70e-12 65.5
Msa0757050 AT5G66210.2 32.353 136 79 4 53 181 374 503 1.70e-12 65.5
Msa0757050 AT4G21940.1 32.353 136 91 1 49 184 407 541 1.72e-12 65.5
Msa0757050 AT4G21940.2 31.884 138 89 2 49 184 407 541 1.81e-12 65.5
Msa0757050 AT1G18890.1 31.138 167 108 6 22 184 341 504 2.65e-12 64.7
Msa0757050 AT3G20410.2 30.882 136 93 1 49 184 397 531 1.35e-11 62.8
Msa0757050 AT3G20410.1 30.882 136 93 1 49 184 397 531 1.35e-11 62.8
Msa0757050 AT5G19450.2 32.847 137 91 1 49 184 363 499 1.52e-11 62.8
Msa0757050 AT5G19450.1 32.847 137 91 1 49 184 363 499 1.52e-11 62.8
Msa0757050 AT1G61950.1 30.986 142 90 3 49 188 405 540 1.72e-11 62.4
Msa0757050 AT3G01830.1 29.286 140 94 3 45 181 8 145 4.18e-11 58.9
Msa0757050 AT1G76040.2 29.412 136 95 1 49 184 417 551 4.73e-11 61.2
Msa0757050 AT2G41100.2 34.821 112 54 4 92 185 20 130 5.09e-11 59.7
Msa0757050 AT2G41860.2 31.818 132 90 0 53 184 364 495 8.63e-11 60.5
Msa0757050 AT1G76040.1 29.412 136 95 1 49 184 179 313 8.95e-11 60.1
Msa0757050 AT1G76040.4 29.412 136 95 1 49 184 179 313 8.95e-11 60.1
Msa0757050 AT2G41860.1 31.818 132 90 0 53 184 364 495 9.31e-11 60.5

Find 32 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCATTTCATCTTTCTT+TGG 0.177414 5_2:+11737145 None:intergenic
TTTCAAGTATTTGATATAAA+TGG 0.216373 5_2:-11736902 Msa0757050:CDS
CATAGACTTTAAAGAGTTCA+TGG 0.277650 5_2:-11736978 Msa0757050:CDS
AAAAGCTACCATAAAACTTT+AGG 0.335712 5_2:+11737257 None:intergenic
CCTTAGTGCTTGTAAGAAAA+TGG 0.341545 5_2:-11736816 Msa0757050:CDS
CAAAGAAAGATGAAATGAAA+TGG 0.359646 5_2:-11737144 Msa0757050:CDS
AGCTGCAAAGGCTTTGGATA+AGG 0.388502 5_2:-11737062 Msa0757050:CDS
TAAAGCAGCTGCAAAGGCTT+TGG 0.392833 5_2:-11737068 Msa0757050:CDS
AATTGTCTCAAATATTTAAG+AGG 0.413219 5_2:-11736856 Msa0757050:CDS
TCTCAAATATTTAAGAGGTT+AGG 0.421604 5_2:-11736851 Msa0757050:CDS
GCTGCAAAGGCTTTGGATAA+GGG 0.422855 5_2:-11737061 Msa0757050:CDS
TCATTTCATCTTTCTTTGGT+TGG 0.424902 5_2:+11737149 None:intergenic
CAAAGATGGCAAGATTAGTC+TGG 0.440035 5_2:-11737098 Msa0757050:CDS
AAAGAAAGATGAAATGAAAT+GGG 0.477872 5_2:-11737143 Msa0757050:CDS
TAGATTTGAATGAGTTTACA+AGG 0.501078 5_2:-11736760 Msa0757050:CDS
AAAATGGTGAAAGGTGTTGA+TGG 0.531030 5_2:-11736800 Msa0757050:CDS
ATGGAAATGTTCAATGGAGA+AGG 0.540301 5_2:-11736959 Msa0757050:CDS
CTGCAAAGGCTTTGGATAAG+GGG 0.540527 5_2:-11737060 Msa0757050:CDS
AGGCAATAAGATAAAAGAAG+AGG 0.542613 5_2:-11736939 Msa0757050:CDS
GTATTTGATATAAATGGTGA+TGG 0.547891 5_2:-11736896 Msa0757050:CDS
GAGTTCATGGAAATGTTCAA+TGG 0.548455 5_2:-11736965 Msa0757050:CDS
AGAGTATAAAGCAGCTGCAA+AGG 0.556821 5_2:-11737074 Msa0757050:CDS
GGTGTTGATGGTGATGGAGA+TGG 0.571699 5_2:-11736788 Msa0757050:CDS
AGTGAAAGCATTTAAAGCAA+TGG 0.579623 5_2:-11737020 Msa0757050:CDS
GTGAAAGGTGTTGATGGTGA+TGG 0.580268 5_2:-11736794 Msa0757050:CDS
AAATTCGACACAAACAAAGA+TGG 0.592536 5_2:-11737112 Msa0757050:CDS
TTTACAAGGATGATGATGAA+TGG 0.601621 5_2:-11736746 Msa0757050:CDS
TATTTGATATAAATGGTGAT+GGG 0.605312 5_2:-11736895 Msa0757050:CDS
TGCAAAGGCTTTGGATAAGG+GGG 0.606980 5_2:-11737059 Msa0757050:CDS
GCAATGGACTCTGACAAAGA+TGG 0.615789 5_2:-11737004 Msa0757050:CDS
GCTTGTAAGAAAATGGTGAA+AGG 0.660596 5_2:-11736809 Msa0757050:CDS
TGATGGGAAAATTAGTGCTG+AGG 0.690315 5_2:-11736879 Msa0757050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAAGTATTTGATATAAA+TGG - chr5_2:11737102-11737121 Msa0757050:CDS 15.0%
!! AAAGAAAGATGAAATGAAAT+GGG - chr5_2:11736861-11736880 Msa0757050:CDS 20.0%
!! TATTTGATATAAATGGTGAT+GGG - chr5_2:11737109-11737128 Msa0757050:CDS 20.0%
!! AATTGTCTCAAATATTTAAG+AGG - chr5_2:11737148-11737167 Msa0757050:CDS 20.0%
!! TTGAGTTTCCTAAAGTTTTA+TGG - chr5_2:11736739-11736758 Msa0757050:CDS 25.0%
! AAAAGCTACCATAAAACTTT+AGG + chr5_2:11736750-11736769 None:intergenic 25.0%
!!! TTTTGAGCTATATTTTTTCG+TGG + chr5_2:11736790-11736809 None:intergenic 25.0%
! CATTTCATTTCATCTTTCTT+TGG + chr5_2:11736862-11736881 None:intergenic 25.0%
! CAAAGAAAGATGAAATGAAA+TGG - chr5_2:11736860-11736879 Msa0757050:CDS 25.0%
! GTATTTGATATAAATGGTGA+TGG - chr5_2:11737108-11737127 Msa0757050:CDS 25.0%
! TCTCAAATATTTAAGAGGTT+AGG - chr5_2:11737153-11737172 Msa0757050:CDS 25.0%
! TAGATTTGAATGAGTTTACA+AGG - chr5_2:11737244-11737263 Msa0757050:CDS 25.0%
TCATTTCATCTTTCTTTGGT+TGG + chr5_2:11736858-11736877 None:intergenic 30.0%
AAATTCGACACAAACAAAGA+TGG - chr5_2:11736892-11736911 Msa0757050:CDS 30.0%
!! AGTGAAAGCATTTAAAGCAA+TGG - chr5_2:11736984-11737003 Msa0757050:CDS 30.0%
! CATAGACTTTAAAGAGTTCA+TGG - chr5_2:11737026-11737045 Msa0757050:CDS 30.0%
AGGCAATAAGATAAAAGAAG+AGG - chr5_2:11737065-11737084 Msa0757050:CDS 30.0%
! TTTACAAGGATGATGATGAA+TGG - chr5_2:11737258-11737277 Msa0757050:CDS 30.0%
!!! ATATTTTTTCGTGGAGAGGT+TGG + chr5_2:11736781-11736800 None:intergenic 35.0%
!!! AGCTATATTTTTTCGTGGAG+AGG + chr5_2:11736785-11736804 None:intergenic 35.0%
GAGTTCATGGAAATGTTCAA+TGG - chr5_2:11737039-11737058 Msa0757050:CDS 35.0%
ATGGAAATGTTCAATGGAGA+AGG - chr5_2:11737045-11737064 Msa0757050:CDS 35.0%
! CCATTTTCTTACAAGCACTA+AGG + chr5_2:11737191-11737210 None:intergenic 35.0%
CCTTAGTGCTTGTAAGAAAA+TGG - chr5_2:11737188-11737207 Msa0757050:CDS 35.0%
GCTTGTAAGAAAATGGTGAA+AGG - chr5_2:11737195-11737214 Msa0757050:CDS 35.0%
!! AAAATGGTGAAAGGTGTTGA+TGG - chr5_2:11737204-11737223 Msa0757050:CDS 35.0%
! CAAAGATGGCAAGATTAGTC+TGG - chr5_2:11736906-11736925 Msa0757050:CDS 40.0%
AGAGTATAAAGCAGCTGCAA+AGG - chr5_2:11736930-11736949 Msa0757050:CDS 40.0%
TGATGGGAAAATTAGTGCTG+AGG - chr5_2:11737125-11737144 Msa0757050:CDS 40.0%
TAAAGCAGCTGCAAAGGCTT+TGG - chr5_2:11736936-11736955 Msa0757050:CDS 45.0%
!! AGCTGCAAAGGCTTTGGATA+AGG - chr5_2:11736942-11736961 Msa0757050:CDS 45.0%
!! GCTGCAAAGGCTTTGGATAA+GGG - chr5_2:11736943-11736962 Msa0757050:CDS 45.0%
!! CTGCAAAGGCTTTGGATAAG+GGG - chr5_2:11736944-11736963 Msa0757050:CDS 45.0%
!! TGCAAAGGCTTTGGATAAGG+GGG - chr5_2:11736945-11736964 Msa0757050:CDS 45.0%
! GCAATGGACTCTGACAAAGA+TGG - chr5_2:11737000-11737019 Msa0757050:CDS 45.0%
!! GTGAAAGGTGTTGATGGTGA+TGG - chr5_2:11737210-11737229 Msa0757050:CDS 45.0%
!! TTTTCGTGGAGAGGTTGGCT+TGG + chr5_2:11736776-11736795 None:intergenic 50.0%
!! AAGGCTTTGGATAAGGGGGT+TGG - chr5_2:11736949-11736968 Msa0757050:CDS 50.0%
!! AGGCTTTGGATAAGGGGGTT+GGG - chr5_2:11736950-11736969 Msa0757050:CDS 50.0%
!! GGTGTTGATGGTGATGGAGA+TGG - chr5_2:11737216-11737235 Msa0757050:CDS 50.0%
!! GGCTTTGGATAAGGGGGTTG+GGG - chr5_2:11736951-11736970 Msa0757050:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 11736730 11737296 11736730 ID=Msa0757050;Name=Msa0757050
chr5_2 mRNA 11736730 11737296 11736730 ID=Msa0757050-mRNA-1;Parent=Msa0757050;Name=Msa0757050-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|188
chr5_2 exon 11736730 11737296 11736730 ID=Msa0757050-mRNA-1:exon:5289;Parent=Msa0757050-mRNA-1
chr5_2 CDS 11736730 11737296 11736730 ID=Msa0757050-mRNA-1:cds;Parent=Msa0757050-mRNA-1
Gene Sequence

>Msa0757050

ATGTCAAAATTGAGTTTCCTAAAGTTTTATGGTAGCTTTTCAGCCAAGCCAACCTCTCCACGAAAAAATATAGCTCAAAAGAACAGCTTCAAGTCGAGACAAACATCGATTACTGAAATGAGTTTCCAACCAAAGAAAGATGAAATGAAATGGGTGTTTGAGAAATTCGACACAAACAAAGATGGCAAGATTAGTCTGGAAGAGTATAAAGCAGCTGCAAAGGCTTTGGATAAGGGGGTTGGGGATACTGATGCAGTGAAAGCATTTAAAGCAATGGACTCTGACAAAGATGGCTTCATAGACTTTAAAGAGTTCATGGAAATGTTCAATGGAGAAGGCAATAAGATAAAAGAAGAGGAGATTAAGAGTGCTTTTCAAGTATTTGATATAAATGGTGATGGGAAAATTAGTGCTGAGGAATTGTCTCAAATATTTAAGAGGTTAGGAGAGAGTTGTAGCCTTAGTGCTTGTAAGAAAATGGTGAAAGGTGTTGATGGTGATGGAGATGGTCTTATAGATTTGAATGAGTTTACAAGGATGATGATGAATGGCAAGAAATGTGCCTAA
Protein sequence

>Msa0757050

MSKLSFLKFYGSFSAKPTSPRKNIAQKNSFKSRQTSITEMSFQPKKDEMKWVFEKFDTNKDGKISLEEYKAAAKALDKGVGDTDAVKAFKAMDSDKDGFIDFKEFMEMFNGEGNKIKEEEIKSAFQVFDINGDGKISAEELSQIFKRLGESCSLSACKKMVKGVDGDGDGLIDLNEFTRMMMNGKKCA*