AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0757080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0757080 MtrunA17_Chr5g0402331 77.419 124 4 2 1 100 66 189 3.75e-56 172
Msa0757080 MtrunA17_Chr5g0402361 77.419 124 4 2 1 100 238 361 2.39e-54 172
Msa0757080 MtrunA17_Chr5g0402361 75.410 122 7 1 1 99 66 187 6.57e-54 171
Msa0757080 MtrunA17_Chr5g0402351 93.750 48 3 0 1 48 41 88 3.98e-26 93.2
Msa0757080 MtrunA17_Chr4g0044961 38.596 114 48 2 2 93 76 189 6.92e-19 77.0
Msa0757080 MtrunA17_Chr3g0111371 34.234 111 53 1 3 93 25 135 2.85e-15 66.2
Msa0757080 MtrunA17_Chr1g0161211 37.895 95 35 1 24 94 87 181 6.77e-15 66.6
Msa0757080 MtrunA17_Chr8g0391691 38.384 99 37 1 24 98 77 175 9.89e-13 60.8
Msa0757080 MtrunA17_Chr5g0434951 35.789 95 38 1 22 93 43 137 3.86e-12 58.2
Msa0757080 MtrunA17_Chr8g0392951 30.000 120 61 2 1 97 42 161 6.73e-12 58.2
Msa0757080 MtrunA17_Chr3g0116151 32.456 114 54 2 3 93 26 139 8.58e-12 57.4
Msa0757080 MtrunA17_Chr3g0116141 32.456 114 54 2 3 93 25 138 9.56e-12 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0757080 AT2G15680.1 40.708 113 45 2 3 93 70 182 1.64e-21 84.0
Msa0757080 AT5G37770.1 33.871 124 54 1 1 96 35 158 3.68e-16 69.7
Msa0757080 AT5G37770.2 33.871 124 54 1 1 96 35 158 3.68e-16 69.7
Msa0757080 AT1G24620.1 33.333 120 56 1 3 98 57 176 2.41e-15 68.2
Msa0757080 AT1G66400.1 32.500 120 55 1 1 94 33 152 2.33e-14 64.7
Msa0757080 AT1G18210.2 32.381 105 51 1 9 93 49 153 9.13e-14 63.5
Msa0757080 AT1G18210.1 32.381 105 51 1 9 93 49 153 9.13e-14 63.5
Msa0757080 AT5G42380.1 30.769 117 56 1 2 93 68 184 2.90e-13 62.4
Msa0757080 AT3G10190.1 36.170 94 46 2 1 93 125 205 6.92e-12 59.3
Msa0757080 AT4G14640.2 27.731 119 62 1 1 95 13 131 2.38e-11 56.6
Msa0757080 AT4G14640.1 27.731 119 62 1 1 95 31 149 9.12e-11 55.5

Find 27 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAGAGTCCATGGCTTTAA+AGG 0.172462 5_2:-11793889 None:intergenic
CATAGACTTTAAAGAGTTCA+TGG 0.277650 5_2:+11793923 Msa0757080:CDS
CCTTAGTGCTTGTAAGAAAA+TGG 0.341545 5_2:+11794086 Msa0757080:CDS
GCTGCAAAAGCTTTGGATAA+GGG 0.402200 5_2:+11793840 Msa0757080:CDS
TAAAGCAGCTGCAAAAGCTT+TGG 0.411228 5_2:+11793833 Msa0757080:CDS
AATTGTCTCAAATATTTAAG+AGG 0.413219 5_2:+11794046 Msa0757080:CDS
TCTCAAATATTTAAGAGGTT+AGG 0.421604 5_2:+11794051 Msa0757080:CDS
AAGCTTTGGATAAGGGGATT+GGG 0.425029 5_2:+11793847 Msa0757080:CDS
AGCTGCAAAAGCTTTGGATA+AGG 0.434736 5_2:+11793839 Msa0757080:CDS
AAAGCTTTGGATAAGGGGAT+TGG 0.463563 5_2:+11793846 Msa0757080:CDS
GCCATCTTTGTCAGAGTCCA+TGG 0.488944 5_2:-11793898 None:intergenic
CTGCAAAAGCTTTGGATAAG+GGG 0.499395 5_2:+11793841 Msa0757080:CDS
TAGATTTGAATGAGTTTACA+AGG 0.501078 5_2:+11794142 Msa0757080:CDS
ATGGAAATGTTCAATGGAGA+AGG 0.503364 5_2:+11793942 Msa0757080:CDS
AAAATGGTGAAAGGTGTTGA+TGG 0.531030 5_2:+11794102 Msa0757080:CDS
AGCTTTGGATAAGGGGATTG+GGG 0.547088 5_2:+11793848 Msa0757080:CDS
GAGTTCATGGAAATGTTCAA+TGG 0.548455 5_2:+11793936 Msa0757080:CDS
AATGGCAAGAAATGTGCCTA+AGG 0.556472 5_2:+11794174 Msa0757080:CDS
GGTGTTGATGGTGATGGAGA+TGG 0.571699 5_2:+11794114 Msa0757080:CDS
GTGAAAGGTGTTGATGGTGA+TGG 0.580268 5_2:+11794108 Msa0757080:CDS
TTTACAAGGATGATGATGAA+TGG 0.601621 5_2:+11794156 Msa0757080:CDS
TGGAAATGTTCAATGGAGAA+GGG 0.618853 5_2:+11793943 Msa0757080:CDS
AGTGAAAGCCTTTAAAGCCA+TGG 0.622176 5_2:+11793881 Msa0757080:CDS
GCCATGGACTCTGACAAAGA+TGG 0.626952 5_2:+11793897 Msa0757080:CDS
GCTTGTAAGAAAATGGTGAA+AGG 0.660596 5_2:+11794093 Msa0757080:CDS
AAATGTTCAATGGAGAAGGG+TGG 0.668240 5_2:+11793946 Msa0757080:CDS
TGATGGGAAAATCAGTGCTG+AGG 0.731787 5_2:+11794023 Msa0757080:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAAGTATTTGATATAAA+TGG + chr5_2:11794000-11794019 Msa0757080:intron 15.0%
!! TATTTGATATAAATGGTGAT+GGG + chr5_2:11794007-11794026 Msa0757080:intron 20.0%
!! AATTGTCTCAAATATTTAAG+AGG + chr5_2:11794046-11794065 Msa0757080:CDS 20.0%
! GTATTTGATATAAATGGTGA+TGG + chr5_2:11794006-11794025 Msa0757080:intron 25.0%
! TCTCAAATATTTAAGAGGTT+AGG + chr5_2:11794051-11794070 Msa0757080:CDS 25.0%
! TAGATTTGAATGAGTTTACA+AGG + chr5_2:11794142-11794161 Msa0757080:CDS 25.0%
! CATAGACTTTAAAGAGTTCA+TGG + chr5_2:11793923-11793942 Msa0757080:CDS 30.0%
! TTTACAAGGATGATGATGAA+TGG + chr5_2:11794156-11794175 Msa0757080:CDS 30.0%
GAGTTCATGGAAATGTTCAA+TGG + chr5_2:11793936-11793955 Msa0757080:CDS 35.0%
ATGGAAATGTTCAATGGAGA+AGG + chr5_2:11793942-11793961 Msa0757080:CDS 35.0%
TGGAAATGTTCAATGGAGAA+GGG + chr5_2:11793943-11793962 Msa0757080:CDS 35.0%
! CCATTTTCTTACAAGCACTA+AGG - chr5_2:11794089-11794108 None:intergenic 35.0%
CCTTAGTGCTTGTAAGAAAA+TGG + chr5_2:11794086-11794105 Msa0757080:CDS 35.0%
GCTTGTAAGAAAATGGTGAA+AGG + chr5_2:11794093-11794112 Msa0757080:CDS 35.0%
!! AAAATGGTGAAAGGTGTTGA+TGG + chr5_2:11794102-11794121 Msa0757080:CDS 35.0%
TAAAGCAGCTGCAAAAGCTT+TGG + chr5_2:11793833-11793852 Msa0757080:CDS 40.0%
!! AGCTGCAAAAGCTTTGGATA+AGG + chr5_2:11793839-11793858 Msa0757080:CDS 40.0%
!! GCTGCAAAAGCTTTGGATAA+GGG + chr5_2:11793840-11793859 Msa0757080:CDS 40.0%
!! CTGCAAAAGCTTTGGATAAG+GGG + chr5_2:11793841-11793860 Msa0757080:CDS 40.0%
!! AAAGCTTTGGATAAGGGGAT+TGG + chr5_2:11793846-11793865 Msa0757080:CDS 40.0%
!! AAGCTTTGGATAAGGGGATT+GGG + chr5_2:11793847-11793866 Msa0757080:CDS 40.0%
!! AGTGAAAGCCTTTAAAGCCA+TGG + chr5_2:11793881-11793900 Msa0757080:CDS 40.0%
AAATGTTCAATGGAGAAGGG+TGG + chr5_2:11793946-11793965 Msa0757080:CDS 40.0%
GGGTGGTAAGATAAAAGAAG+AGG + chr5_2:11793963-11793982 Msa0757080:intron 40.0%
!! AGCTTTGGATAAGGGGATTG+GGG + chr5_2:11793848-11793867 Msa0757080:CDS 45.0%
!! GTCAGAGTCCATGGCTTTAA+AGG - chr5_2:11793892-11793911 None:intergenic 45.0%
TGATGGGAAAATCAGTGCTG+AGG + chr5_2:11794023-11794042 Msa0757080:intron 45.0%
!! GTGAAAGGTGTTGATGGTGA+TGG + chr5_2:11794108-11794127 Msa0757080:CDS 45.0%
GCCATGGACTCTGACAAAGA+TGG + chr5_2:11793897-11793916 Msa0757080:CDS 50.0%
GCCATCTTTGTCAGAGTCCA+TGG - chr5_2:11793901-11793920 None:intergenic 50.0%
!! GGTGTTGATGGTGATGGAGA+TGG + chr5_2:11794114-11794133 Msa0757080:CDS 50.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 11793822 11794194 11793822 ID=Msa0757080;Name=Msa0757080
chr5_2 mRNA 11793822 11794194 11793822 ID=Msa0757080-mRNA-1;Parent=Msa0757080;Name=Msa0757080-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0|1|1|1|2|0|100
chr5_2 exon 11793822 11793967 11793822 ID=Msa0757080-mRNA-1:exon:5294;Parent=Msa0757080-mRNA-1
chr5_2 exon 11794038 11794194 11794038 ID=Msa0757080-mRNA-1:exon:5295;Parent=Msa0757080-mRNA-1
chr5_2 CDS 11793822 11793967 11793822 ID=Msa0757080-mRNA-1:cds;Parent=Msa0757080-mRNA-1
chr5_2 CDS 11794038 11794194 11794038 ID=Msa0757080-mRNA-1:cds;Parent=Msa0757080-mRNA-1
Gene Sequence

>Msa0757080

CTGGAAGAGTATAAAGCAGCTGCAAAAGCTTTGGATAAGGGGATTGGGGATACTGATGCAGTGAAAGCCTTTAAAGCCATGGACTCTGACAAAGATGGCTTCATAGACTTTAAAGAGTTCATGGAAATGTTCAATGGAGAAGGGTGTGCTGAGGAATTGTCTCAAATATTTAAGAGGTTAGGAGAGAGTTGTAGCCTTAGTGCTTGTAAGAAAATGGTGAAAGGTGTTGATGGTGATGGAGATGGTCTTATAGATTTGAATGAGTTTACAAGGATGATGATGAATGGCAAGAAATGTGCCTAA

Protein sequence

>Msa0757080

LEEYKAAAKALDKGIGDTDAVKAFKAMDSDKDGFIDFKEFMEMFNGEGCAEELSQIFKRLGESCSLSACKKMVKGVDGDGDGLIDLNEFTRMMMNGKKCA*