AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0714960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0714960 MtrunA17_Chr5g0402361 93.548 186 12 0 1 186 1 186 7.53e-122 348
Msa0714960 MtrunA17_Chr5g0402361 89.714 175 17 1 15 188 187 361 2.80e-107 311
Msa0714960 MtrunA17_Chr5g0402331 89.947 189 18 1 1 188 1 189 1.13e-119 336
Msa0714960 MtrunA17_Chr4g0044961 47.368 190 90 4 1 181 1 189 5.50e-48 155
Msa0714960 MtrunA17_Chr5g0402351 88.235 85 10 0 32 116 7 91 4.01e-46 147
Msa0714960 MtrunA17_Chr1g0161211 39.888 178 96 5 8 182 12 181 8.74e-34 119
Msa0714960 MtrunA17_Chr3g0111371 41.176 136 77 1 46 181 3 135 2.94e-32 113
Msa0714960 MtrunA17_Chr5g0434951 42.222 135 78 0 47 181 3 137 1.21e-30 108
Msa0714960 MtrunA17_Chr8g0391691 44.681 141 75 2 48 186 36 175 1.84e-30 110
Msa0714960 MtrunA17_Chr8g0392951 38.849 139 85 0 47 185 23 161 8.27e-29 105
Msa0714960 MtrunA17_Chr1g0169581 36.957 138 84 1 47 181 3 140 1.83e-26 98.6
Msa0714960 MtrunA17_Chr6g0482731 36.765 136 85 1 47 181 25 160 3.35e-26 98.6
Msa0714960 MtrunA17_Chr7g0245911 38.554 166 85 5 32 185 34 194 4.08e-26 99.0
Msa0714960 MtrunA17_Chr8g0351681 39.189 148 81 3 48 186 49 196 6.87e-26 98.6
Msa0714960 MtrunA17_Chr4g0033601 36.879 141 86 2 47 184 4 144 2.01e-23 90.9
Msa0714960 MtrunA17_Chr7g0256171 39.583 144 84 2 48 188 5 148 9.99e-23 89.0
Msa0714960 MtrunA17_Chr7g0225921 35.252 139 89 1 45 182 3 141 1.43e-22 88.2
Msa0714960 MtrunA17_Chr1g0169621 34.146 164 78 3 47 181 3 165 2.29e-22 88.6
Msa0714960 MtrunA17_Chr1g0188171 36.957 138 80 2 50 183 14 148 5.09e-22 87.0
Msa0714960 MtrunA17_Chr3g0116151 34.091 132 87 0 50 181 8 139 8.43e-22 86.3
Msa0714960 MtrunA17_Chr3g0116141 34.091 132 87 0 50 181 7 138 8.76e-22 86.3
Msa0714960 MtrunA17_Chr6g0459231 34.351 131 86 0 52 182 12 142 1.12e-21 85.9
Msa0714960 MtrunA17_Chr2g0329371 37.762 143 84 2 47 184 63 205 1.24e-21 87.8
Msa0714960 MtrunA17_Chr3g0121191 36.429 140 78 2 48 186 93 222 2.13e-21 87.4
Msa0714960 MtrunA17_Chr7g0256701 35.714 140 84 3 48 181 73 212 2.34e-21 87.0
Msa0714960 MtrunA17_Chr3g0093271 32.258 186 113 4 1 182 22 198 4.92e-21 85.9
Msa0714960 MtrunA17_Chr1g0185001 32.877 146 95 1 40 182 1 146 1.48e-20 83.2
Msa0714960 MtrunA17_Chr1g0166281 35.971 139 82 2 48 182 12 147 3.30e-20 82.4
Msa0714960 MtrunA17_Chr7g0254731 35.252 139 83 2 48 182 12 147 1.83e-19 80.5
Msa0714960 MtrunA17_Chr4g0057661 32.836 134 90 0 48 181 6 139 4.68e-19 79.3
Msa0714960 MtrunA17_Chr5g0440931 35.252 139 83 2 48 182 12 147 7.76e-19 79.0
Msa0714960 MtrunA17_Chr6g0460211 35.252 139 83 2 48 182 12 147 8.81e-19 78.6
Msa0714960 MtrunA17_Chr4g0057641 32.836 134 90 0 48 181 6 139 1.03e-18 78.2
Msa0714960 MtrunA17_Chr8g0373691 30.597 134 92 1 49 181 54 187 1.23e-18 79.3
Msa0714960 MtrunA17_Chr8g0373691 42.188 64 37 0 118 181 51 114 2.96e-11 59.7
Msa0714960 MtrunA17_Chr4g0064231 35.443 158 83 3 46 184 62 219 7.02e-18 78.2
Msa0714960 MtrunA17_Chr4g0057651 33.088 136 90 1 48 183 6 140 9.38e-18 75.9
Msa0714960 MtrunA17_Chr7g0274101 36.364 132 83 1 53 183 17 148 4.22e-17 74.3
Msa0714960 MtrunA17_Chr7g0245231 34.507 142 91 2 43 183 25 165 1.03e-16 73.9
Msa0714960 MtrunA17_Chr6g0450921 32.903 155 101 3 30 183 12 164 1.71e-16 73.2
Msa0714960 MtrunA17_Chr4g0062221 27.536 138 97 1 47 181 10 147 4.40e-16 72.0
Msa0714960 MtrunA17_Chr8g0366401 28.261 138 95 1 48 181 17 154 4.49e-16 72.0
Msa0714960 MtrunA17_Chr8g0368681 28.859 149 98 4 40 181 1 148 1.02e-15 70.9
Msa0714960 MtrunA17_Chr3g0125311 30.719 153 83 5 48 182 13 160 5.74e-15 68.9
Msa0714960 MtrunA17_Chr7g0267751 32.847 137 88 3 49 184 441 574 9.67e-15 71.6
Msa0714960 MtrunA17_Chr5g0441691 31.618 136 92 1 49 184 374 508 9.94e-15 71.6
Msa0714960 MtrunA17_Chr1g0176751 33.333 138 86 4 49 184 430 563 1.29e-14 71.2
Msa0714960 MtrunA17_Chr1g0157131 31.159 138 90 2 49 184 380 514 1.39e-14 71.2
Msa0714960 MtrunA17_Chr1g0170281 34.783 138 84 4 49 184 424 557 1.52e-14 70.9
Msa0714960 MtrunA17_Chr5g0448721 31.206 141 85 2 49 184 402 535 4.59e-14 69.7
Msa0714960 MtrunA17_Chr0c02g0489761 31.618 136 92 1 49 184 384 518 5.03e-14 69.3
Msa0714960 MtrunA17_Chr1g0160151 31.293 147 84 4 47 182 21 161 6.58e-14 66.2
Msa0714960 MtrunA17_Chr5g0444171 30.882 136 93 1 49 184 398 532 7.40e-14 68.9
Msa0714960 MtrunA17_Chr5g0396261 34.694 98 59 2 87 180 16 112 7.42e-14 65.1
Msa0714960 MtrunA17_Chr4g0068501 29.861 144 96 3 43 181 11 154 7.98e-14 65.9
Msa0714960 MtrunA17_Chr8g0387671 31.159 138 90 2 49 184 372 506 2.05e-13 67.8
Msa0714960 MtrunA17_Chr4g0074811 29.710 138 92 2 49 184 397 531 2.32e-13 67.4
Msa0714960 MtrunA17_Chr1g0200601 33.333 138 86 2 49 184 336 469 2.75e-13 67.4
Msa0714960 MtrunA17_Chr5g0397101 30.435 138 91 2 49 184 372 506 2.89e-13 67.4
Msa0714960 MtrunA17_Chr1g0173541 33.333 132 88 0 53 184 362 493 3.94e-13 67.0
Msa0714960 MtrunA17_Chr8g0385341 32.847 137 88 2 49 184 336 469 4.84e-13 66.6
Msa0714960 MtrunA17_Chr7g0242561 28.986 138 98 0 47 184 360 497 8.15e-13 65.9
Msa0714960 MtrunA17_Chr7g0257751 31.818 132 90 0 53 184 368 499 1.04e-12 65.5
Msa0714960 MtrunA17_Chr5g0405421 32.847 137 77 5 53 181 411 540 2.29e-12 64.7
Msa0714960 MtrunA17_Chr4g0059951 29.091 165 114 3 22 184 351 514 2.54e-12 64.7
Msa0714960 MtrunA17_Chr7g0245371 28.108 185 91 7 18 181 7 170 3.81e-12 62.0
Msa0714960 MtrunA17_Chr3g0131181 31.387 137 90 2 49 184 335 468 2.96e-11 61.2
Msa0714960 MtrunA17_Chr4g0074791 33.088 136 80 2 49 184 401 525 3.78e-11 61.2
Msa0714960 MtrunA17_Chr3g0098951 28.472 144 97 2 49 186 389 532 4.86e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0714960 AT2G15680.1 50.360 139 68 1 43 181 45 182 7.58e-45 147
Msa0714960 AT5G37770.1 41.958 143 78 1 47 184 16 158 2.05e-33 117
Msa0714960 AT5G37770.2 41.958 143 78 1 47 184 16 158 2.05e-33 117
Msa0714960 AT1G24620.1 45.390 141 74 2 48 186 37 176 3.96e-33 117
Msa0714960 AT1G66400.1 40.288 139 80 1 47 182 14 152 5.71e-31 110
Msa0714960 AT1G18210.2 36.709 158 95 2 24 181 1 153 4.78e-30 108
Msa0714960 AT1G18210.1 36.709 158 95 2 24 181 1 153 4.78e-30 108
Msa0714960 AT5G17470.1 43.885 139 74 3 52 186 6 144 7.05e-30 107
Msa0714960 AT5G42380.1 32.597 181 116 2 3 181 8 184 8.51e-29 105
Msa0714960 AT3G03400.1 43.200 125 66 2 52 172 9 132 2.02e-28 103
Msa0714960 AT2G36180.1 42.958 142 76 3 49 186 1 141 1.52e-27 101
Msa0714960 AT1G73630.1 37.241 145 87 2 38 181 9 150 1.54e-26 99.8
Msa0714960 AT2G43290.1 37.241 145 83 2 48 184 65 209 3.00e-24 95.1
Msa0714960 AT3G59440.1 36.691 139 86 1 48 184 51 189 5.30e-24 94.0
Msa0714960 AT1G76650.1 31.638 177 111 1 5 181 10 176 8.47e-24 92.8
Msa0714960 AT1G76650.3 31.638 177 111 1 5 181 10 176 8.47e-24 92.8
Msa0714960 AT3G50770.1 32.184 174 103 3 14 181 39 203 4.75e-23 91.7
Msa0714960 AT4G14640.1 37.143 140 81 2 48 183 13 149 1.25e-22 89.4
Msa0714960 AT1G05990.1 38.298 141 83 2 48 184 5 145 1.05e-21 86.7
Msa0714960 AT3G50360.1 36.957 138 86 1 45 181 24 161 4.42e-21 85.5
Msa0714960 AT3G22930.1 37.324 142 81 3 48 184 35 173 4.70e-21 85.5
Msa0714960 AT1G76640.1 34.641 153 96 2 33 181 6 158 1.89e-20 83.6
Msa0714960 AT3G07490.1 36.111 144 89 2 48 188 5 148 1.92e-20 83.6
Msa0714960 AT4G14640.2 36.508 126 73 2 62 183 9 131 2.19e-20 82.8
Msa0714960 AT4G03290.1 35.417 144 87 2 48 185 5 148 1.47e-19 81.3
Msa0714960 AT5G21274.1 35.971 139 82 2 48 182 12 147 5.84e-19 79.7
Msa0714960 AT1G66410.1 35.252 139 83 2 48 182 12 147 6.43e-19 79.3
Msa0714960 AT5G37780.1 35.252 139 83 2 48 182 12 147 6.43e-19 79.3
Msa0714960 AT2G27030.3 34.965 143 86 2 48 186 12 151 6.91e-19 80.1
Msa0714960 AT3G56800.1 35.971 139 82 2 48 182 12 147 7.79e-19 79.3
Msa0714960 AT2G41110.1 35.971 139 82 2 48 182 12 147 7.79e-19 79.3
Msa0714960 AT2G27030.1 35.971 139 82 2 48 182 12 147 7.79e-19 79.3
Msa0714960 AT2G41410.1 28.889 180 105 6 10 182 45 208 8.61e-19 80.9
Msa0714960 AT3G43810.2 34.694 147 87 3 48 188 12 155 1.02e-18 80.5
Msa0714960 AT3G43810.1 35.252 139 83 2 48 182 12 147 1.22e-18 78.6
Msa0714960 AT4G12860.1 38.028 142 85 2 46 184 3 144 1.32e-18 78.6
Msa0714960 AT3G10190.1 31.818 154 97 3 36 181 52 205 4.81e-18 78.6
Msa0714960 AT1G76650.2 29.379 177 105 2 5 181 10 166 7.93e-18 77.0
Msa0714960 AT3G03410.1 35.338 133 78 1 50 182 4 128 3.07e-17 74.7
Msa0714960 AT4G37010.2 32.143 140 90 2 45 181 28 165 1.11e-16 74.3
Msa0714960 AT1G32250.1 28.767 146 89 3 47 181 15 156 1.13e-16 73.9
Msa0714960 AT4G37010.1 32.143 140 90 2 45 181 24 161 1.28e-16 73.9
Msa0714960 AT1G18530.1 31.884 138 89 3 48 181 7 143 2.90e-16 72.8
Msa0714960 AT5G04870.1 35.507 138 83 4 49 184 456 589 3.53e-16 76.3
Msa0714960 AT2G41110.2 33.113 151 82 3 48 182 12 159 6.33e-16 72.0
Msa0714960 AT5G44460.1 34.320 169 91 6 26 181 13 174 7.34e-16 72.4
Msa0714960 AT4G04695.1 34.058 138 86 2 49 184 337 471 9.02e-16 74.7
Msa0714960 AT5G37780.4 34.400 125 75 2 62 182 13 134 9.54e-16 70.9
Msa0714960 AT1G66410.2 34.400 125 75 2 62 182 36 157 1.12e-15 71.2
Msa0714960 AT2G17290.1 34.559 136 87 1 49 184 391 524 1.32e-15 74.3
Msa0714960 AT2G17290.2 34.559 136 87 1 49 184 391 524 1.32e-15 74.3
Msa0714960 AT5G37780.3 34.400 125 75 2 62 182 52 173 1.44e-15 71.2
Msa0714960 AT5G19360.1 33.088 136 90 1 49 184 374 508 1.58e-15 74.3
Msa0714960 AT5G12180.1 33.088 136 90 1 49 184 379 513 1.74e-15 73.9
Msa0714960 AT5G37780.2 34.400 125 75 2 62 182 41 162 2.05e-15 70.5
Msa0714960 AT5G23580.1 33.835 133 86 1 49 181 328 458 2.76e-15 73.6
Msa0714960 AT1G66410.4 41.053 95 49 2 92 182 20 111 3.81e-15 68.6
Msa0714960 AT1G66410.3 41.053 95 49 2 92 182 20 111 3.81e-15 68.6
Msa0714960 AT3G43810.4 41.053 95 49 2 92 182 20 111 5.94e-15 68.2
Msa0714960 AT3G43810.3 41.053 95 49 2 92 182 20 111 5.94e-15 68.2
Msa0714960 AT2G41100.7 32.692 156 86 4 48 185 66 220 7.40e-15 71.2
Msa0714960 AT2G41100.6 32.692 156 86 4 48 185 66 220 7.40e-15 71.2
Msa0714960 AT2G41100.3 32.692 156 86 4 48 185 66 220 7.40e-15 71.2
Msa0714960 AT4G04720.2 32.609 138 88 2 49 184 385 519 8.88e-15 72.0
Msa0714960 AT4G04720.1 32.609 138 88 2 49 184 385 519 8.88e-15 72.0
Msa0714960 AT2G41100.4 32.692 156 86 4 48 185 101 255 9.62e-15 71.2
Msa0714960 AT2G41100.4 31.126 151 87 3 48 181 12 162 2.13e-12 64.7
Msa0714960 AT2G41100.1 32.692 156 86 4 48 185 101 255 9.62e-15 71.2
Msa0714960 AT2G41100.1 31.126 151 87 3 48 181 12 162 2.13e-12 64.7
Msa0714960 AT3G10660.2 34.307 137 86 3 49 184 492 625 1.01e-14 72.0
Msa0714960 AT3G10660.1 34.307 137 86 3 49 184 492 625 1.01e-14 72.0
Msa0714960 AT4G38230.1 34.559 136 87 1 49 184 186 319 1.02e-14 71.2
Msa0714960 AT3G03000.1 30.714 140 93 3 48 184 20 158 1.19e-14 68.6
Msa0714960 AT4G04710.2 33.824 136 89 1 49 184 338 472 1.33e-14 71.6
Msa0714960 AT4G04710.1 33.824 136 89 1 49 184 351 485 1.34e-14 71.6
Msa0714960 AT4G38230.2 34.559 136 87 1 49 184 360 493 1.38e-14 71.6
Msa0714960 AT4G04710.3 33.824 136 89 1 49 184 324 458 1.38e-14 71.2
Msa0714960 AT4G38230.4 34.559 136 87 1 49 184 330 463 1.55e-14 71.2
Msa0714960 AT4G38230.3 34.559 136 87 1 49 184 330 463 1.55e-14 71.2
Msa0714960 AT3G25600.1 28.986 138 93 3 48 181 12 148 2.36e-14 67.8
Msa0714960 AT5G12480.2 33.577 137 90 1 49 184 271 407 3.10e-14 70.5
Msa0714960 AT4G09570.1 34.559 136 87 2 49 184 331 464 3.20e-14 70.5
Msa0714960 AT4G04700.1 33.835 133 87 1 49 181 337 468 3.86e-14 70.1
Msa0714960 AT5G12480.1 33.577 137 90 1 49 184 365 501 3.88e-14 70.1
Msa0714960 AT3G51850.1 28.659 164 116 1 22 184 332 495 4.25e-14 70.1
Msa0714960 AT1G35670.1 33.824 136 88 2 49 184 332 465 5.87e-14 69.7
Msa0714960 AT2G41090.1 31.852 135 90 1 48 182 12 144 6.27e-14 67.4
Msa0714960 AT1G21550.1 31.293 147 90 4 47 183 9 154 9.38e-14 65.9
Msa0714960 AT2G17890.1 30.625 160 94 4 29 181 400 549 9.52e-14 68.9
Msa0714960 AT4G35310.2 32.353 136 90 1 49 184 403 536 1.15e-13 68.9
Msa0714960 AT4G35310.1 32.353 136 90 1 49 184 403 536 1.15e-13 68.9
Msa0714960 AT2G38910.1 34.559 136 85 3 49 183 440 572 1.26e-13 68.6
Msa0714960 AT4G23650.1 31.884 138 89 2 49 184 384 518 1.34e-13 68.6
Msa0714960 AT4G04740.4 30.435 138 91 2 49 184 374 508 1.91e-13 68.2
Msa0714960 AT4G04740.6 30.435 138 91 2 49 184 374 508 1.91e-13 68.2
Msa0714960 AT4G04740.5 30.435 138 91 2 49 184 374 508 1.91e-13 68.2
Msa0714960 AT4G04740.3 30.435 138 91 2 49 184 374 508 1.91e-13 68.2
Msa0714960 AT4G04740.1 30.435 138 91 2 49 184 374 508 1.91e-13 68.2
Msa0714960 AT4G04740.7 30.435 138 91 2 49 184 374 508 1.91e-13 68.2
Msa0714960 AT4G04710.4 33.333 138 89 2 49 184 351 487 2.59e-13 67.8
Msa0714960 AT4G36070.1 33.566 143 80 5 47 181 377 512 2.59e-13 67.8
Msa0714960 AT4G36070.2 33.566 143 80 5 47 181 377 512 2.81e-13 67.8
Msa0714960 AT3G57530.1 33.083 133 86 2 53 184 373 503 1.03e-12 66.2
Msa0714960 AT1G50700.1 31.159 138 90 2 49 184 379 513 1.06e-12 65.9
Msa0714960 AT5G66210.1 33.824 136 77 5 53 181 374 503 1.07e-12 65.9
Msa0714960 AT5G66210.2 33.824 136 77 5 53 181 374 503 1.07e-12 65.9
Msa0714960 AT4G20780.1 29.379 177 97 4 26 181 14 183 1.27e-12 63.9
Msa0714960 AT4G21940.2 31.884 138 89 2 49 184 407 541 1.64e-12 65.5
Msa0714960 AT4G21940.1 31.884 138 89 2 49 184 407 541 1.77e-12 65.5
Msa0714960 AT1G18890.1 30.539 167 109 6 22 184 341 504 8.96e-12 63.2
Msa0714960 AT1G61950.1 30.986 142 90 3 49 188 405 540 1.78e-11 62.4
Msa0714960 AT3G20410.2 30.435 138 91 2 49 184 397 531 2.03e-11 62.4
Msa0714960 AT3G20410.1 30.435 138 91 2 49 184 397 531 2.03e-11 62.4
Msa0714960 AT5G19450.2 32.847 137 91 1 49 184 363 499 2.20e-11 62.0
Msa0714960 AT5G19450.1 32.847 137 91 1 49 184 363 499 2.20e-11 62.0
Msa0714960 AT3G01830.1 29.286 140 94 3 45 181 8 145 3.97e-11 58.9
Msa0714960 AT1G76040.2 28.986 138 93 2 49 184 417 551 4.46e-11 61.2

Find 34 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCATTTCATCTTTCTT+TGG 0.177414 5_1:-7800341 None:intergenic
TTTCAAGTATTTGATATAAA+TGG 0.216373 5_1:+7800584 Msa0714960:CDS
CATAGACTTTAAAGAGTTCA+TGG 0.277650 5_1:+7800508 Msa0714960:CDS
CCTTAATGCTTGTAAGAAAA+TGG 0.334724 5_1:+7800670 Msa0714960:CDS
AAAAGCTACCATAAAACTTT+AGG 0.335712 5_1:-7800229 None:intergenic
CAAAGAAAGATGAAATGAAA+TGG 0.359646 5_1:+7800342 Msa0714960:CDS
AGCTGCAAAGGCTTTGGATA+AGG 0.388502 5_1:+7800424 Msa0714960:CDS
TAAAGCAGCTGCAAAGGCTT+TGG 0.392833 5_1:+7800418 Msa0714960:CDS
AATTGTCTCAAATATTTAAG+AGG 0.413219 5_1:+7800630 Msa0714960:CDS
TCTCAAATATTTAAGAGGTT+AGG 0.421604 5_1:+7800635 Msa0714960:CDS
GCTGCAAAGGCTTTGGATAA+GGG 0.422855 5_1:+7800425 Msa0714960:CDS
TCATTTCATCTTTCTTTGGT+TGG 0.424902 5_1:-7800337 None:intergenic
CTTGTAAGAAAATGGTGAAA+GGG 0.436913 5_1:+7800678 Msa0714960:CDS
CAAAGATGGCAAGATTAGTC+TGG 0.440035 5_1:+7800388 Msa0714960:CDS
GTGAAAGGGGTTGACGGTGA+TGG 0.473054 5_1:+7800692 Msa0714960:CDS
AAAGAAAGATGAAATGAAAT+GGG 0.477872 5_1:+7800343 Msa0714960:CDS
TAGATTTGAATGAGTTTACA+AGG 0.501078 5_1:+7800726 Msa0714960:CDS
AAAATGGTGAAAGGGGTTGA+CGG 0.504340 5_1:+7800686 Msa0714960:CDS
ATGGAAATGTTCAATGGAGA+AGG 0.540301 5_1:+7800527 Msa0714960:CDS
CTGCAAAGGCTTTGGATAAG+GGG 0.540527 5_1:+7800426 Msa0714960:CDS
AGGCAATAAGATAAAAGAAG+AGG 0.542613 5_1:+7800547 Msa0714960:CDS
GTATTTGATATAAATGGTGA+TGG 0.547891 5_1:+7800590 Msa0714960:CDS
GAGTTCATGGAAATGTTCAA+TGG 0.548455 5_1:+7800521 Msa0714960:CDS
GCTTGTAAGAAAATGGTGAA+AGG 0.549619 5_1:+7800677 Msa0714960:CDS
AGAGTATAAAGCAGCTGCAA+AGG 0.556821 5_1:+7800412 Msa0714960:CDS
GGGGTTGACGGTGATGGAGA+TGG 0.573082 5_1:+7800698 Msa0714960:CDS
AGTGAAAGCATTTAAAGCAA+TGG 0.579623 5_1:+7800466 Msa0714960:CDS
AAATTCGACACAAACAAAGA+TGG 0.592536 5_1:+7800374 Msa0714960:CDS
TTTACAAGGATGATGATGAA+TGG 0.601621 5_1:+7800740 Msa0714960:CDS
TATTTGATATAAATGGTGAT+GGG 0.605312 5_1:+7800591 Msa0714960:CDS
TGCAAAGGCTTTGGATAAGG+GGG 0.606980 5_1:+7800427 Msa0714960:CDS
GCAATGGACTCTGACAAAGA+TGG 0.615789 5_1:+7800482 Msa0714960:CDS
TTGTAAGAAAATGGTGAAAG+GGG 0.649196 5_1:+7800679 Msa0714960:CDS
TGATGGGAAAATTAGTGCTG+AGG 0.690315 5_1:+7800607 Msa0714960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAAGTATTTGATATAAA+TGG + chr5_1:7800584-7800603 Msa0714960:CDS 15.0%
!!! TTTTGAGCTATATTTTTTTG+TGG - chr5_1:7800272-7800291 None:intergenic 20.0%
!! AAAGAAAGATGAAATGAAAT+GGG + chr5_1:7800343-7800362 Msa0714960:CDS 20.0%
!! TATTTGATATAAATGGTGAT+GGG + chr5_1:7800591-7800610 Msa0714960:CDS 20.0%
!! AATTGTCTCAAATATTTAAG+AGG + chr5_1:7800630-7800649 Msa0714960:CDS 20.0%
!! TTGAGTTTCCTAAAGTTTTA+TGG + chr5_1:7800221-7800240 Msa0714960:CDS 25.0%
! AAAAGCTACCATAAAACTTT+AGG - chr5_1:7800232-7800251 None:intergenic 25.0%
! CATTTCATTTCATCTTTCTT+TGG - chr5_1:7800344-7800363 None:intergenic 25.0%
! CAAAGAAAGATGAAATGAAA+TGG + chr5_1:7800342-7800361 Msa0714960:CDS 25.0%
! GTATTTGATATAAATGGTGA+TGG + chr5_1:7800590-7800609 Msa0714960:CDS 25.0%
! TCTCAAATATTTAAGAGGTT+AGG + chr5_1:7800635-7800654 Msa0714960:CDS 25.0%
! TAGATTTGAATGAGTTTACA+AGG + chr5_1:7800726-7800745 Msa0714960:CDS 25.0%
!!! ATATTTTTTTGTGGAGAGGT+TGG - chr5_1:7800263-7800282 None:intergenic 30.0%
!!! AGCTATATTTTTTTGTGGAG+AGG - chr5_1:7800267-7800286 None:intergenic 30.0%
TCATTTCATCTTTCTTTGGT+TGG - chr5_1:7800340-7800359 None:intergenic 30.0%
AAATTCGACACAAACAAAGA+TGG + chr5_1:7800374-7800393 Msa0714960:CDS 30.0%
!! AGTGAAAGCATTTAAAGCAA+TGG + chr5_1:7800466-7800485 Msa0714960:CDS 30.0%
! CATAGACTTTAAAGAGTTCA+TGG + chr5_1:7800508-7800527 Msa0714960:CDS 30.0%
AGGCAATAAGATAAAAGAAG+AGG + chr5_1:7800547-7800566 Msa0714960:CDS 30.0%
! CCATTTTCTTACAAGCATTA+AGG - chr5_1:7800673-7800692 None:intergenic 30.0%
CCTTAATGCTTGTAAGAAAA+TGG + chr5_1:7800670-7800689 Msa0714960:CDS 30.0%
CTTGTAAGAAAATGGTGAAA+GGG + chr5_1:7800678-7800697 Msa0714960:CDS 30.0%
TTGTAAGAAAATGGTGAAAG+GGG + chr5_1:7800679-7800698 Msa0714960:CDS 30.0%
! TTTACAAGGATGATGATGAA+TGG + chr5_1:7800740-7800759 Msa0714960:CDS 30.0%
GAGTTCATGGAAATGTTCAA+TGG + chr5_1:7800521-7800540 Msa0714960:CDS 35.0%
ATGGAAATGTTCAATGGAGA+AGG + chr5_1:7800527-7800546 Msa0714960:CDS 35.0%
GCTTGTAAGAAAATGGTGAA+AGG + chr5_1:7800677-7800696 Msa0714960:CDS 35.0%
! CAAAGATGGCAAGATTAGTC+TGG + chr5_1:7800388-7800407 Msa0714960:CDS 40.0%
AGAGTATAAAGCAGCTGCAA+AGG + chr5_1:7800412-7800431 Msa0714960:CDS 40.0%
TGATGGGAAAATTAGTGCTG+AGG + chr5_1:7800607-7800626 Msa0714960:CDS 40.0%
AAAATGGTGAAAGGGGTTGA+CGG + chr5_1:7800686-7800705 Msa0714960:CDS 40.0%
!! TTTTTGTGGAGAGGTTGGCT+TGG - chr5_1:7800258-7800277 None:intergenic 45.0%
TAAAGCAGCTGCAAAGGCTT+TGG + chr5_1:7800418-7800437 Msa0714960:CDS 45.0%
!! AGCTGCAAAGGCTTTGGATA+AGG + chr5_1:7800424-7800443 Msa0714960:CDS 45.0%
!! GCTGCAAAGGCTTTGGATAA+GGG + chr5_1:7800425-7800444 Msa0714960:CDS 45.0%
!! CTGCAAAGGCTTTGGATAAG+GGG + chr5_1:7800426-7800445 Msa0714960:CDS 45.0%
!! TGCAAAGGCTTTGGATAAGG+GGG + chr5_1:7800427-7800446 Msa0714960:CDS 45.0%
! GCAATGGACTCTGACAAAGA+TGG + chr5_1:7800482-7800501 Msa0714960:CDS 45.0%
!! AAGGCTTTGGATAAGGGGGT+TGG + chr5_1:7800431-7800450 Msa0714960:CDS 50.0%
!! AGGCTTTGGATAAGGGGGTT+GGG + chr5_1:7800432-7800451 Msa0714960:CDS 50.0%
!! GGCTTTGGATAAGGGGGTTG+GGG + chr5_1:7800433-7800452 Msa0714960:CDS 55.0%
GTGAAAGGGGTTGACGGTGA+TGG + chr5_1:7800692-7800711 Msa0714960:CDS 55.0%
!! GGGGTTGACGGTGATGGAGA+TGG + chr5_1:7800698-7800717 Msa0714960:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 7800212 7800778 7800212 ID=Msa0714960;Name=Msa0714960
chr5_1 mRNA 7800212 7800778 7800212 ID=Msa0714960-mRNA-1;Parent=Msa0714960;Name=Msa0714960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|188
chr5_1 exon 7800212 7800778 7800212 ID=Msa0714960-mRNA-1:exon:3266;Parent=Msa0714960-mRNA-1
chr5_1 CDS 7800212 7800778 7800212 ID=Msa0714960-mRNA-1:cds;Parent=Msa0714960-mRNA-1
Gene Sequence

>Msa0714960

ATGTCAAAATTGAGTTTCCTAAAGTTTTATGGTAGCTTTTCAGCCAAGCCAACCTCTCCACAAAAAAATATAGCTCAAAAGAACAGCTTCAAGTCGAGACAAACATCGATTACTGAAATGAGTTTCCAACCAAAGAAAGATGAAATGAAATGGGTGTTTGAGAAATTCGACACAAACAAAGATGGCAAGATTAGTCTGGAAGAGTATAAAGCAGCTGCAAAGGCTTTGGATAAGGGGGTTGGGGATACTGATGCAGTGAAAGCATTTAAAGCAATGGACTCTGACAAAGATGGCTTCATAGACTTTAAAGAGTTCATGGAAATGTTCAATGGAGAAGGCAATAAGATAAAAGAAGAGGAGATTAAGAGTGCTTTTCAAGTATTTGATATAAATGGTGATGGGAAAATTAGTGCTGAGGAATTGTCTCAAATATTTAAGAGGTTAGGAGAGAGTTGTAGCCTTAATGCTTGTAAGAAAATGGTGAAAGGGGTTGACGGTGATGGAGATGGTCTTATAGATTTGAATGAGTTTACAAGGATGATGATGAATGGCAAGAAATGTGCCTAA
Protein sequence

>Msa0714960

MSKLSFLKFYGSFSAKPTSPQKNIAQKNSFKSRQTSITEMSFQPKKDEMKWVFEKFDTNKDGKISLEEYKAAAKALDKGVGDTDAVKAFKAMDSDKDGFIDFKEFMEMFNGEGNKIKEEEIKSAFQVFDINGDGKISAEELSQIFKRLGESCSLNACKKMVKGVDGDGDGLIDLNEFTRMMMNGKKCA*