AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0798030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0798030 MtrunA17_Chr5g0402361 95.161 186 9 0 1 186 1 186 4.62e-124 354
Msa0798030 MtrunA17_Chr5g0402361 90.857 175 15 1 15 188 187 361 1.44e-108 315
Msa0798030 MtrunA17_Chr5g0402331 91.534 189 15 1 1 188 1 189 7.61e-122 342
Msa0798030 MtrunA17_Chr4g0044961 46.316 190 92 3 1 181 1 189 1.54e-48 156
Msa0798030 MtrunA17_Chr5g0402351 88.235 85 10 0 32 116 7 91 2.92e-46 147
Msa0798030 MtrunA17_Chr1g0161211 39.888 178 96 5 8 182 12 181 2.94e-34 120
Msa0798030 MtrunA17_Chr3g0111371 41.176 136 77 1 46 181 3 135 2.81e-32 113
Msa0798030 MtrunA17_Chr8g0391691 44.681 141 75 2 48 186 36 175 6.53e-31 111
Msa0798030 MtrunA17_Chr5g0434951 41.481 135 79 0 47 181 3 137 7.69e-30 107
Msa0798030 MtrunA17_Chr8g0392951 38.849 139 85 0 47 185 23 161 7.42e-29 105
Msa0798030 MtrunA17_Chr1g0169581 36.957 138 84 1 47 181 3 140 1.72e-26 98.6
Msa0798030 MtrunA17_Chr6g0482731 36.765 136 85 1 47 181 25 160 3.04e-26 99.0
Msa0798030 MtrunA17_Chr7g0245911 37.989 179 91 7 20 185 23 194 3.16e-26 99.4
Msa0798030 MtrunA17_Chr8g0351681 41.216 148 78 4 48 186 49 196 7.24e-26 98.6
Msa0798030 MtrunA17_Chr4g0033601 36.879 141 86 2 47 184 4 144 1.46e-23 91.3
Msa0798030 MtrunA17_Chr1g0169621 34.756 164 77 3 47 181 3 165 6.24e-23 90.1
Msa0798030 MtrunA17_Chr7g0256171 39.583 144 84 2 48 188 5 148 8.88e-23 89.4
Msa0798030 MtrunA17_Chr7g0225921 35.252 139 89 1 45 182 3 141 3.27e-22 87.4
Msa0798030 MtrunA17_Chr1g0188171 36.957 138 80 2 50 183 14 148 4.20e-22 87.4
Msa0798030 MtrunA17_Chr2g0329371 37.762 143 84 2 47 184 63 205 9.87e-22 88.2
Msa0798030 MtrunA17_Chr6g0459231 34.351 131 86 0 52 182 12 142 2.04e-21 85.5
Msa0798030 MtrunA17_Chr3g0116151 34.091 132 87 0 50 181 8 139 2.16e-21 85.1
Msa0798030 MtrunA17_Chr3g0116141 34.091 132 87 0 50 181 7 138 2.35e-21 85.1
Msa0798030 MtrunA17_Chr7g0256701 35.714 140 84 3 48 181 73 212 2.65e-21 87.0
Msa0798030 MtrunA17_Chr3g0121191 36.429 140 78 2 48 186 93 222 4.48e-21 86.7
Msa0798030 MtrunA17_Chr1g0185001 32.877 146 95 1 40 182 1 146 1.41e-20 83.6
Msa0798030 MtrunA17_Chr3g0093271 31.720 186 114 4 1 182 22 198 2.37e-20 84.0
Msa0798030 MtrunA17_Chr1g0166281 35.971 139 82 2 48 182 12 147 2.42e-20 82.8
Msa0798030 MtrunA17_Chr7g0254731 35.252 139 83 2 48 182 12 147 1.59e-19 80.5
Msa0798030 MtrunA17_Chr4g0057661 32.836 134 90 0 48 181 6 139 5.26e-19 79.0
Msa0798030 MtrunA17_Chr5g0440931 35.252 139 83 2 48 182 12 147 7.36e-19 79.0
Msa0798030 MtrunA17_Chr6g0460211 35.252 139 83 2 48 182 12 147 8.81e-19 78.6
Msa0798030 MtrunA17_Chr8g0373691 30.597 134 92 1 49 181 54 187 9.94e-19 79.7
Msa0798030 MtrunA17_Chr8g0373691 42.188 64 37 0 118 181 51 114 2.37e-11 60.1
Msa0798030 MtrunA17_Chr4g0057641 32.836 134 90 0 48 181 6 139 1.22e-18 78.2
Msa0798030 MtrunA17_Chr4g0064231 35.443 158 83 3 46 184 62 219 5.24e-18 78.6
Msa0798030 MtrunA17_Chr4g0057651 33.088 136 90 1 48 183 6 140 9.48e-18 75.9
Msa0798030 MtrunA17_Chr7g0274101 36.364 132 83 1 53 183 17 148 4.45e-17 74.3
Msa0798030 MtrunA17_Chr7g0245231 34.507 142 91 2 43 183 25 165 7.94e-17 74.3
Msa0798030 MtrunA17_Chr6g0450921 32.903 155 101 3 30 183 12 164 1.49e-16 73.6
Msa0798030 MtrunA17_Chr4g0062221 27.536 138 97 1 47 181 10 147 4.44e-16 72.0
Msa0798030 MtrunA17_Chr8g0366401 28.261 138 95 1 48 181 17 154 5.84e-16 71.6
Msa0798030 MtrunA17_Chr8g0368681 28.859 149 98 4 40 181 1 148 1.30e-15 70.9
Msa0798030 MtrunA17_Chr3g0125311 30.719 153 83 5 48 182 13 160 5.01e-15 69.3
Msa0798030 MtrunA17_Chr7g0267751 32.847 137 88 3 49 184 441 574 5.35e-15 72.4
Msa0798030 MtrunA17_Chr1g0170281 34.307 137 86 3 49 184 424 557 7.77e-15 72.0
Msa0798030 MtrunA17_Chr1g0176751 33.333 138 86 4 49 184 430 563 1.07e-14 71.6
Msa0798030 MtrunA17_Chr5g0441691 31.618 136 92 1 49 184 374 508 1.31e-14 71.2
Msa0798030 MtrunA17_Chr5g0396261 35.714 98 58 2 87 180 16 112 1.73e-14 66.6
Msa0798030 MtrunA17_Chr1g0157131 31.159 138 90 2 49 184 380 514 2.26e-14 70.5
Msa0798030 MtrunA17_Chr5g0448721 31.618 136 91 1 49 184 402 535 4.38e-14 69.7
Msa0798030 MtrunA17_Chr0c02g0489761 31.429 140 95 1 49 188 384 522 5.64e-14 69.3
Msa0798030 MtrunA17_Chr1g0160151 31.293 147 84 4 47 182 21 161 5.75e-14 66.6
Msa0798030 MtrunA17_Chr4g0068501 29.861 144 96 3 43 181 11 154 5.90e-14 66.2
Msa0798030 MtrunA17_Chr5g0444171 30.882 136 93 1 49 184 398 532 6.85e-14 68.9
Msa0798030 MtrunA17_Chr1g0200601 32.353 136 90 1 49 184 336 469 2.00e-13 67.8
Msa0798030 MtrunA17_Chr5g0397101 30.435 138 91 2 49 184 372 506 2.23e-13 67.4
Msa0798030 MtrunA17_Chr4g0074811 29.710 138 92 2 49 184 397 531 2.44e-13 67.4
Msa0798030 MtrunA17_Chr1g0173541 33.333 132 88 0 53 184 362 493 3.11e-13 67.0
Msa0798030 MtrunA17_Chr8g0385341 33.088 136 89 1 49 184 336 469 4.03e-13 66.6
Msa0798030 MtrunA17_Chr8g0387671 30.435 138 91 2 49 184 372 506 6.81e-13 66.2
Msa0798030 MtrunA17_Chr7g0257751 31.818 132 90 0 53 184 368 499 8.72e-13 65.9
Msa0798030 MtrunA17_Chr7g0242561 28.986 138 98 0 47 184 360 497 1.02e-12 65.5
Msa0798030 MtrunA17_Chr5g0405421 28.571 161 96 5 29 181 391 540 3.20e-12 64.3
Msa0798030 MtrunA17_Chr7g0245371 28.108 185 91 7 18 181 7 170 8.40e-12 60.8
Msa0798030 MtrunA17_Chr4g0059951 28.485 165 115 3 22 184 351 514 1.32e-11 62.4
Msa0798030 MtrunA17_Chr3g0098951 29.167 144 96 2 49 186 389 532 1.69e-11 62.0
Msa0798030 MtrunA17_Chr4g0074791 33.088 136 80 2 49 184 401 525 1.89e-11 62.0
Msa0798030 MtrunA17_Chr3g0131181 31.618 136 91 1 49 184 335 468 2.19e-11 61.6
Msa0798030 MtrunA17_Chr4g0042491 43.038 79 42 1 103 181 100 175 8.65e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0798030 AT2G15680.1 50.360 139 68 1 43 181 45 182 8.64e-45 147
Msa0798030 AT1G24620.1 45.390 141 74 2 48 186 37 176 1.20e-33 118
Msa0798030 AT5G37770.1 41.958 143 78 1 47 184 16 158 1.97e-33 117
Msa0798030 AT5G37770.2 41.958 143 78 1 47 184 16 158 1.97e-33 117
Msa0798030 AT1G66400.1 40.288 139 80 1 47 182 14 152 4.45e-31 110
Msa0798030 AT5G17470.1 43.885 139 74 3 52 186 6 144 2.32e-30 108
Msa0798030 AT1G18210.2 36.709 158 95 2 24 181 1 153 4.20e-30 108
Msa0798030 AT1G18210.1 36.709 158 95 2 24 181 1 153 4.20e-30 108
Msa0798030 AT3G03400.1 43.200 125 66 2 52 172 9 132 2.51e-28 103
Msa0798030 AT2G36180.1 42.958 142 76 3 49 186 1 141 1.29e-27 101
Msa0798030 AT5G42380.1 32.044 181 117 2 3 181 8 184 1.45e-27 102
Msa0798030 AT1G73630.1 37.241 145 87 2 38 181 9 150 1.37e-26 99.8
Msa0798030 AT3G59440.1 36.691 139 86 1 48 184 51 189 3.93e-24 94.4
Msa0798030 AT2G43290.1 37.241 145 83 2 48 184 65 209 4.53e-24 94.7
Msa0798030 AT3G50770.1 32.759 174 102 3 14 181 39 203 8.41e-24 93.6
Msa0798030 AT1G76650.1 32.184 174 111 2 14 181 4 176 2.12e-23 92.0
Msa0798030 AT1G76650.3 32.184 174 111 2 14 181 4 176 2.12e-23 92.0
Msa0798030 AT4G14640.1 37.143 140 81 2 48 183 13 149 1.14e-22 89.4
Msa0798030 AT1G05990.1 38.298 141 83 2 48 184 5 145 1.41e-21 86.3
Msa0798030 AT3G50360.1 36.957 138 86 1 45 181 24 161 1.43e-21 87.0
Msa0798030 AT3G22930.1 37.324 142 81 3 48 184 35 173 3.46e-21 85.9
Msa0798030 AT3G07490.1 36.111 144 89 2 48 188 5 148 1.38e-20 84.0
Msa0798030 AT4G14640.2 36.508 126 73 2 62 183 9 131 1.96e-20 82.8
Msa0798030 AT1G76640.1 34.641 153 96 2 33 181 6 158 3.22e-20 83.2
Msa0798030 AT4G03290.1 35.417 144 87 2 48 185 5 148 1.32e-19 81.3
Msa0798030 AT2G27030.3 34.965 143 86 2 48 186 12 151 5.54e-19 80.5
Msa0798030 AT5G21274.1 35.971 139 82 2 48 182 12 147 5.54e-19 79.7
Msa0798030 AT1G66410.1 35.252 139 83 2 48 182 12 147 5.78e-19 79.7
Msa0798030 AT5G37780.1 35.252 139 83 2 48 182 12 147 5.78e-19 79.7
Msa0798030 AT3G56800.1 35.971 139 82 2 48 182 12 147 7.15e-19 79.3
Msa0798030 AT2G41110.1 35.971 139 82 2 48 182 12 147 7.15e-19 79.3
Msa0798030 AT2G27030.1 35.971 139 82 2 48 182 12 147 7.15e-19 79.3
Msa0798030 AT3G43810.2 34.694 147 87 3 48 188 12 155 7.71e-19 80.9
Msa0798030 AT4G12860.1 38.028 142 85 2 46 184 3 144 9.50e-19 79.0
Msa0798030 AT3G43810.1 35.252 139 83 2 48 182 12 147 1.22e-18 78.6
Msa0798030 AT2G41410.1 30.573 157 98 5 36 182 53 208 2.96e-18 79.3
Msa0798030 AT3G10190.1 31.818 154 97 3 36 181 52 205 1.21e-17 77.4
Msa0798030 AT1G76650.2 29.885 174 105 3 14 181 4 166 1.79e-17 76.3
Msa0798030 AT3G03410.1 35.338 133 78 1 50 182 4 128 2.79e-17 74.7
Msa0798030 AT1G32250.1 28.169 142 95 1 47 181 15 156 5.29e-17 74.7
Msa0798030 AT5G04870.1 35.507 138 83 4 49 184 456 589 2.50e-16 76.6
Msa0798030 AT1G18530.1 31.884 138 89 3 48 181 7 143 3.50e-16 72.4
Msa0798030 AT2G41110.2 33.113 151 82 3 48 182 12 159 4.62e-16 72.4
Msa0798030 AT4G04695.1 34.559 136 88 1 49 184 337 471 5.91e-16 75.5
Msa0798030 AT4G37010.2 31.429 140 91 2 45 181 28 165 6.81e-16 72.0
Msa0798030 AT5G37780.4 34.400 125 75 2 62 182 13 134 6.95e-16 71.2
Msa0798030 AT4G37010.1 31.429 140 91 2 45 181 24 161 7.18e-16 72.0
Msa0798030 AT5G44460.1 34.320 169 91 6 26 181 13 174 8.41e-16 72.0
Msa0798030 AT1G66410.2 34.400 125 75 2 62 182 36 157 1.04e-15 71.2
Msa0798030 AT2G17290.1 34.559 136 87 1 49 184 391 524 1.06e-15 74.7
Msa0798030 AT2G17290.2 34.559 136 87 1 49 184 391 524 1.06e-15 74.7
Msa0798030 AT5G23580.1 33.835 133 86 1 49 181 328 458 1.10e-15 74.7
Msa0798030 AT5G37780.3 34.400 125 75 2 62 182 52 173 1.29e-15 71.6
Msa0798030 AT5G37780.2 34.400 125 75 2 62 182 41 162 1.65e-15 70.9
Msa0798030 AT2G41100.7 32.692 156 86 4 48 185 66 220 2.02e-15 72.8
Msa0798030 AT2G41100.6 32.692 156 86 4 48 185 66 220 2.02e-15 72.8
Msa0798030 AT2G41100.3 32.692 156 86 4 48 185 66 220 2.02e-15 72.8
Msa0798030 AT5G19360.1 33.088 136 90 1 49 184 374 508 2.50e-15 73.6
Msa0798030 AT5G12180.1 33.088 136 90 1 49 184 379 513 2.51e-15 73.6
Msa0798030 AT1G66410.4 41.053 95 49 2 92 182 20 111 2.86e-15 68.9
Msa0798030 AT1G66410.3 41.053 95 49 2 92 182 20 111 2.86e-15 68.9
Msa0798030 AT2G41100.4 32.692 156 86 4 48 185 101 255 3.08e-15 72.8
Msa0798030 AT2G41100.4 31.126 151 87 3 48 181 12 162 7.57e-13 65.9
Msa0798030 AT2G41100.1 32.692 156 86 4 48 185 101 255 3.08e-15 72.8
Msa0798030 AT2G41100.1 31.126 151 87 3 48 181 12 162 7.57e-13 65.9
Msa0798030 AT3G10660.2 34.307 137 86 3 49 184 492 625 3.70e-15 73.2
Msa0798030 AT3G10660.1 34.307 137 86 3 49 184 492 625 3.70e-15 73.2
Msa0798030 AT3G43810.4 41.053 95 49 2 92 182 20 111 4.66e-15 68.6
Msa0798030 AT3G43810.3 41.053 95 49 2 92 182 20 111 4.66e-15 68.6
Msa0798030 AT4G04720.2 33.088 136 90 1 49 184 385 519 8.47e-15 72.0
Msa0798030 AT4G04720.1 33.088 136 90 1 49 184 385 519 8.47e-15 72.0
Msa0798030 AT4G38230.1 34.559 136 87 1 49 184 186 319 9.97e-15 71.2
Msa0798030 AT3G03000.1 30.714 140 93 3 48 184 20 158 1.35e-14 68.6
Msa0798030 AT4G38230.4 34.559 136 87 1 49 184 330 463 1.39e-14 71.6
Msa0798030 AT4G38230.3 34.559 136 87 1 49 184 330 463 1.39e-14 71.6
Msa0798030 AT4G38230.2 34.559 136 87 1 49 184 360 493 1.44e-14 71.2
Msa0798030 AT4G09570.1 34.559 136 87 2 49 184 331 464 1.70e-14 71.2
Msa0798030 AT5G12480.2 33.577 137 90 1 49 184 271 407 2.13e-14 70.9
Msa0798030 AT3G25600.1 28.986 138 93 3 48 181 12 148 2.81e-14 67.4
Msa0798030 AT5G12480.1 33.577 137 90 1 49 184 365 501 2.97e-14 70.5
Msa0798030 AT1G35670.1 33.824 136 88 2 49 184 332 465 3.78e-14 70.1
Msa0798030 AT4G04700.1 33.835 133 87 1 49 181 337 468 4.09e-14 70.1
Msa0798030 AT3G51850.1 28.659 164 116 1 22 184 332 495 4.86e-14 70.1
Msa0798030 AT2G41090.1 31.852 135 90 1 48 182 12 144 6.01e-14 67.4
Msa0798030 AT1G21550.1 31.293 147 90 4 47 183 9 154 6.44e-14 66.6
Msa0798030 AT2G38910.1 34.559 136 85 3 49 183 440 572 7.76e-14 69.3
Msa0798030 AT4G04710.2 33.088 136 90 1 49 184 338 472 7.76e-14 69.3
Msa0798030 AT4G04710.3 33.088 136 90 1 49 184 324 458 9.03e-14 68.9
Msa0798030 AT4G04710.1 33.088 136 90 1 49 184 351 485 9.40e-14 68.9
Msa0798030 AT4G23650.1 31.884 138 89 2 49 184 384 518 1.09e-13 68.9
Msa0798030 AT4G35310.2 32.353 136 90 1 49 184 403 536 1.09e-13 68.9
Msa0798030 AT4G35310.1 32.353 136 90 1 49 184 403 536 1.09e-13 68.9
Msa0798030 AT2G17890.1 30.625 160 94 4 29 181 400 549 1.24e-13 68.9
Msa0798030 AT4G04740.4 30.147 136 94 1 49 184 374 508 2.05e-13 68.2
Msa0798030 AT4G04740.6 30.147 136 94 1 49 184 374 508 2.05e-13 68.2
Msa0798030 AT4G04740.5 30.147 136 94 1 49 184 374 508 2.05e-13 68.2
Msa0798030 AT4G04740.3 30.147 136 94 1 49 184 374 508 2.05e-13 68.2
Msa0798030 AT4G04740.1 30.147 136 94 1 49 184 374 508 2.05e-13 68.2
Msa0798030 AT4G04740.7 30.147 136 94 1 49 184 374 508 2.05e-13 68.2
Msa0798030 AT4G36070.1 33.566 143 80 5 47 181 377 512 2.55e-13 67.8
Msa0798030 AT4G36070.2 33.566 143 80 5 47 181 377 512 2.95e-13 67.8
Msa0798030 AT1G50700.1 31.618 136 92 1 49 184 379 513 5.91e-13 66.6
Msa0798030 AT3G57530.1 33.083 133 86 2 53 184 373 503 8.57e-13 66.2
Msa0798030 AT4G20780.1 29.379 177 97 4 26 181 14 183 1.02e-12 63.9
Msa0798030 AT4G21940.1 32.353 136 91 1 49 184 407 541 1.38e-12 65.9
Msa0798030 AT4G21940.2 32.353 136 91 1 49 184 407 541 1.40e-12 65.9
Msa0798030 AT4G04710.4 32.609 138 90 2 49 184 351 487 1.70e-12 65.5
Msa0798030 AT5G66210.1 32.353 136 79 4 53 181 374 503 2.06e-12 65.1
Msa0798030 AT5G66210.2 32.353 136 79 4 53 181 374 503 2.06e-12 65.1
Msa0798030 AT1G18890.1 31.138 167 108 6 22 184 341 504 2.38e-12 65.1
Msa0798030 AT3G20410.2 30.882 136 93 1 49 184 397 531 1.36e-11 62.8
Msa0798030 AT3G20410.1 30.882 136 93 1 49 184 397 531 1.36e-11 62.8
Msa0798030 AT5G19450.2 32.847 137 91 1 49 184 363 499 1.70e-11 62.4
Msa0798030 AT5G19450.1 32.847 137 91 1 49 184 363 499 1.70e-11 62.4
Msa0798030 AT1G61950.1 30.986 142 90 3 49 188 405 540 1.94e-11 62.4
Msa0798030 AT3G01830.1 29.286 140 94 3 45 181 8 145 4.09e-11 58.9
Msa0798030 AT2G41100.2 34.821 112 54 4 92 185 20 130 4.84e-11 59.7
Msa0798030 AT1G76040.2 29.412 136 95 1 49 184 417 551 5.50e-11 61.2
Msa0798030 AT2G41860.2 31.818 132 90 0 53 184 364 495 8.47e-11 60.5
Msa0798030 AT2G41860.1 31.818 132 90 0 53 184 364 495 8.63e-11 60.5

Find 33 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCATTTCATCTTTCTT+TGG 0.177414 5_3:+10538193 None:intergenic
TTTCAAGTATTTGATATAAA+TGG 0.216373 5_3:-10537950 Msa0798030:CDS
CATAGACTTTAAAGAGTTCA+TGG 0.277650 5_3:-10538026 Msa0798030:CDS
CCTTAGTGCTTGTAAGAAAA+TGG 0.341545 5_3:-10537864 Msa0798030:CDS
AAAAGCTACCATGAAACTTT+AGG 0.342938 5_3:+10538305 None:intergenic
TTGAGTTTCCTAAAGTTTCA+TGG 0.347349 5_3:-10538313 Msa0798030:CDS
CAAAGAAAGATGAAATGAAA+TGG 0.359646 5_3:-10538192 Msa0798030:CDS
AGCTGCAAAGGCTTTGGATA+AGG 0.388502 5_3:-10538110 Msa0798030:CDS
TAAAGCAGCTGCAAAGGCTT+TGG 0.392833 5_3:-10538116 Msa0798030:CDS
AATTGTCTCAAATATTTAAG+AGG 0.413219 5_3:-10537904 Msa0798030:CDS
TCTCAAATATTTAAGAGGTT+AGG 0.421604 5_3:-10537899 Msa0798030:CDS
GCTGCAAAGGCTTTGGATAA+GGG 0.422855 5_3:-10538109 Msa0798030:CDS
TCATTTCATCTTTCTTTGGT+TGG 0.424902 5_3:+10538197 None:intergenic
CAAAGATGGCAAGATTAGTC+TGG 0.440035 5_3:-10538146 Msa0798030:CDS
AAAGAAAGATGAAATGAAAT+GGG 0.477872 5_3:-10538191 Msa0798030:CDS
TAGATTTGAATGAGTTTACA+AGG 0.501078 5_3:-10537808 Msa0798030:CDS
AAAATGGTGAAAGGTGTTGA+TGG 0.531030 5_3:-10537848 Msa0798030:CDS
ATGGAAATGTTCAATGGAGA+AGG 0.540301 5_3:-10538007 Msa0798030:CDS
CTGCAAAGGCTTTGGATAAG+GGG 0.540527 5_3:-10538108 Msa0798030:CDS
AGGCAATAAGATAAAAGAAG+AGG 0.542613 5_3:-10537987 Msa0798030:CDS
GTATTTGATATAAATGGTGA+TGG 0.547891 5_3:-10537944 Msa0798030:CDS
GAGTTCATGGAAATGTTCAA+TGG 0.548455 5_3:-10538013 Msa0798030:CDS
AGAGTATAAAGCAGCTGCAA+AGG 0.556821 5_3:-10538122 Msa0798030:CDS
GGTGTTGATGGTGATGGAGA+TGG 0.571699 5_3:-10537836 Msa0798030:CDS
AGTGAAAGCATTTAAAGCAA+TGG 0.579623 5_3:-10538068 Msa0798030:CDS
GTGAAAGGTGTTGATGGTGA+TGG 0.580268 5_3:-10537842 Msa0798030:CDS
AAATTCGACACAAACAAAGA+TGG 0.592536 5_3:-10538160 Msa0798030:CDS
TTTACAAGGATGATGATGAA+TGG 0.601621 5_3:-10537794 Msa0798030:CDS
TATTTGATATAAATGGTGAT+GGG 0.605312 5_3:-10537943 Msa0798030:CDS
TGCAAAGGCTTTGGATAAGG+GGG 0.606980 5_3:-10538107 Msa0798030:CDS
GCAATGGACTCTGACAAAGA+TGG 0.615789 5_3:-10538052 Msa0798030:CDS
GCTTGTAAGAAAATGGTGAA+AGG 0.660596 5_3:-10537857 Msa0798030:CDS
TGATGGGAAAATTAGTGCTG+AGG 0.690315 5_3:-10537927 Msa0798030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAAGTATTTGATATAAA+TGG - chr5_3:10538150-10538169 Msa0798030:CDS 15.0%
!! AAAGAAAGATGAAATGAAAT+GGG - chr5_3:10537909-10537928 Msa0798030:CDS 20.0%
!! TATTTGATATAAATGGTGAT+GGG - chr5_3:10538157-10538176 Msa0798030:CDS 20.0%
!! AATTGTCTCAAATATTTAAG+AGG - chr5_3:10538196-10538215 Msa0798030:CDS 20.0%
!!! TTTTGAGCTATATTTTTTCG+TGG + chr5_3:10537838-10537857 None:intergenic 25.0%
! CATTTCATTTCATCTTTCTT+TGG + chr5_3:10537910-10537929 None:intergenic 25.0%
! CAAAGAAAGATGAAATGAAA+TGG - chr5_3:10537908-10537927 Msa0798030:CDS 25.0%
! GTATTTGATATAAATGGTGA+TGG - chr5_3:10538156-10538175 Msa0798030:CDS 25.0%
! TCTCAAATATTTAAGAGGTT+AGG - chr5_3:10538201-10538220 Msa0798030:CDS 25.0%
! TAGATTTGAATGAGTTTACA+AGG - chr5_3:10538292-10538311 Msa0798030:CDS 25.0%
TTGAGTTTCCTAAAGTTTCA+TGG - chr5_3:10537787-10537806 Msa0798030:CDS 30.0%
AAAAGCTACCATGAAACTTT+AGG + chr5_3:10537798-10537817 None:intergenic 30.0%
!!! ATATTTTTTCGTGGAGATGT+TGG + chr5_3:10537829-10537848 None:intergenic 30.0%
TCATTTCATCTTTCTTTGGT+TGG + chr5_3:10537906-10537925 None:intergenic 30.0%
AAATTCGACACAAACAAAGA+TGG - chr5_3:10537940-10537959 Msa0798030:CDS 30.0%
!! AGTGAAAGCATTTAAAGCAA+TGG - chr5_3:10538032-10538051 Msa0798030:CDS 30.0%
! CATAGACTTTAAAGAGTTCA+TGG - chr5_3:10538074-10538093 Msa0798030:CDS 30.0%
AGGCAATAAGATAAAAGAAG+AGG - chr5_3:10538113-10538132 Msa0798030:CDS 30.0%
! TTTACAAGGATGATGATGAA+TGG - chr5_3:10538306-10538325 Msa0798030:CDS 30.0%
GAGTTCATGGAAATGTTCAA+TGG - chr5_3:10538087-10538106 Msa0798030:CDS 35.0%
ATGGAAATGTTCAATGGAGA+AGG - chr5_3:10538093-10538112 Msa0798030:CDS 35.0%
! CCATTTTCTTACAAGCACTA+AGG + chr5_3:10538239-10538258 None:intergenic 35.0%
CCTTAGTGCTTGTAAGAAAA+TGG - chr5_3:10538236-10538255 Msa0798030:CDS 35.0%
GCTTGTAAGAAAATGGTGAA+AGG - chr5_3:10538243-10538262 Msa0798030:CDS 35.0%
!! AAAATGGTGAAAGGTGTTGA+TGG - chr5_3:10538252-10538271 Msa0798030:CDS 35.0%
! CAAAGATGGCAAGATTAGTC+TGG - chr5_3:10537954-10537973 Msa0798030:CDS 40.0%
AGAGTATAAAGCAGCTGCAA+AGG - chr5_3:10537978-10537997 Msa0798030:CDS 40.0%
TGATGGGAAAATTAGTGCTG+AGG - chr5_3:10538173-10538192 Msa0798030:CDS 40.0%
!! TTTTCGTGGAGATGTTGGCT+TGG + chr5_3:10537824-10537843 None:intergenic 45.0%
TAAAGCAGCTGCAAAGGCTT+TGG - chr5_3:10537984-10538003 Msa0798030:CDS 45.0%
!! AGCTGCAAAGGCTTTGGATA+AGG - chr5_3:10537990-10538009 Msa0798030:CDS 45.0%
!! GCTGCAAAGGCTTTGGATAA+GGG - chr5_3:10537991-10538010 Msa0798030:CDS 45.0%
!! CTGCAAAGGCTTTGGATAAG+GGG - chr5_3:10537992-10538011 Msa0798030:CDS 45.0%
!! TGCAAAGGCTTTGGATAAGG+GGG - chr5_3:10537993-10538012 Msa0798030:CDS 45.0%
! GCAATGGACTCTGACAAAGA+TGG - chr5_3:10538048-10538067 Msa0798030:CDS 45.0%
!! GTGAAAGGTGTTGATGGTGA+TGG - chr5_3:10538258-10538277 Msa0798030:CDS 45.0%
!! AAGGCTTTGGATAAGGGGGT+TGG - chr5_3:10537997-10538016 Msa0798030:CDS 50.0%
!! AGGCTTTGGATAAGGGGGTT+GGG - chr5_3:10537998-10538017 Msa0798030:CDS 50.0%
!! GGTGTTGATGGTGATGGAGA+TGG - chr5_3:10538264-10538283 Msa0798030:CDS 50.0%
!! GGCTTTGGATAAGGGGGTTG+GGG - chr5_3:10537999-10538018 Msa0798030:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 10537778 10538344 10537778 ID=Msa0798030;Name=Msa0798030
chr5_3 mRNA 10537778 10538344 10537778 ID=Msa0798030-mRNA-1;Parent=Msa0798030;Name=Msa0798030-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|188
chr5_3 exon 10537778 10538344 10537778 ID=Msa0798030-mRNA-1:exon:4406;Parent=Msa0798030-mRNA-1
chr5_3 CDS 10537778 10538344 10537778 ID=Msa0798030-mRNA-1:cds;Parent=Msa0798030-mRNA-1
Gene Sequence

>Msa0798030

ATGTCAAAATTGAGTTTCCTAAAGTTTCATGGTAGCTTTTCAGCCAAGCCAACATCTCCACGAAAAAATATAGCTCAAAAGAACAGCTTCAAGTCGAGACAAACATCGATTACTGAAATGAGTTTCCAACCAAAGAAAGATGAAATGAAATGGGTGTTTGAGAAATTCGACACAAACAAAGATGGCAAGATTAGTCTGGAAGAGTATAAAGCAGCTGCAAAGGCTTTGGATAAGGGGGTTGGGGATACTGATGCAGTGAAAGCATTTAAAGCAATGGACTCTGACAAAGATGGCTTCATAGACTTTAAAGAGTTCATGGAAATGTTCAATGGAGAAGGCAATAAGATAAAAGAAGAGGAGATTAAGAGTGCTTTTCAAGTATTTGATATAAATGGTGATGGGAAAATTAGTGCTGAGGAATTGTCTCAAATATTTAAGAGGTTAGGAGAGAGTTGTAGCCTTAGTGCTTGTAAGAAAATGGTGAAAGGTGTTGATGGTGATGGAGATGGTCTTATAGATTTGAATGAGTTTACAAGGATGATGATGAATGGCAAGAAATGTGCCTAA
Protein sequence

>Msa0798030

MSKLSFLKFHGSFSAKPTSPRKNIAQKNSFKSRQTSITEMSFQPKKDEMKWVFEKFDTNKDGKISLEEYKAAAKALDKGVGDTDAVKAFKAMDSDKDGFIDFKEFMEMFNGEGNKIKEEEIKSAFQVFDINGDGKISAEELSQIFKRLGESCSLSACKKMVKGVDGDGDGLIDLNEFTRMMMNGKKCA*