AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0715110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0715110 MtrunA17_Chr5g0402361 93.714 175 11 0 22 196 12 186 2.56e-113 327
Msa0715110 MtrunA17_Chr5g0402361 86.022 186 25 1 14 198 176 361 3.86e-108 314
Msa0715110 MtrunA17_Chr5g0402331 87.766 188 21 2 12 198 3 189 7.44e-113 319
Msa0715110 MtrunA17_Chr4g0044961 50.993 151 73 1 41 191 40 189 2.05e-47 154
Msa0715110 MtrunA17_Chr5g0402351 84.706 85 13 0 42 126 7 91 2.69e-45 145
Msa0715110 MtrunA17_Chr1g0161211 40.719 167 93 5 28 192 19 181 8.33e-32 114
Msa0715110 MtrunA17_Chr3g0111371 40.441 136 78 1 56 191 3 135 4.06e-31 110
Msa0715110 MtrunA17_Chr8g0391691 43.972 141 76 2 58 196 36 175 2.78e-30 110
Msa0715110 MtrunA17_Chr5g0434951 40.741 135 80 0 57 191 3 137 4.38e-29 105
Msa0715110 MtrunA17_Chr8g0392951 38.129 139 86 0 57 195 23 161 4.63e-29 106
Msa0715110 MtrunA17_Chr6g0482731 36.029 136 86 1 57 191 25 160 3.08e-26 99.0
Msa0715110 MtrunA17_Chr1g0169581 36.232 138 85 1 57 191 3 140 7.73e-26 97.1
Msa0715110 MtrunA17_Chr7g0245911 36.264 182 96 6 27 195 20 194 5.02e-25 96.7
Msa0715110 MtrunA17_Chr8g0351681 38.514 148 82 3 58 196 49 196 1.97e-24 95.1
Msa0715110 MtrunA17_Chr4g0033601 36.879 141 86 2 57 194 4 144 2.36e-23 90.9
Msa0715110 MtrunA17_Chr2g0329371 33.668 199 115 6 1 194 19 205 3.40e-23 92.0
Msa0715110 MtrunA17_Chr7g0256171 39.583 144 84 2 58 198 5 148 1.08e-22 89.4
Msa0715110 MtrunA17_Chr1g0169621 32.515 163 82 1 57 191 3 165 6.15e-22 87.8
Msa0715110 MtrunA17_Chr7g0225921 34.848 132 85 1 62 192 10 141 2.80e-21 85.5
Msa0715110 MtrunA17_Chr1g0188171 36.496 137 80 2 60 192 14 147 4.59e-21 85.1
Msa0715110 MtrunA17_Chr3g0121191 37.324 142 74 3 58 196 93 222 1.07e-20 85.9
Msa0715110 MtrunA17_Chr3g0093271 31.694 183 111 5 15 192 25 198 1.97e-20 84.7
Msa0715110 MtrunA17_Chr6g0459231 32.824 131 88 0 62 192 12 142 2.09e-20 83.2
Msa0715110 MtrunA17_Chr3g0116141 33.333 132 88 0 60 191 7 138 3.30e-20 82.4
Msa0715110 MtrunA17_Chr3g0116151 33.333 132 88 0 60 191 8 139 3.68e-20 82.4
Msa0715110 MtrunA17_Chr7g0256701 35.000 140 85 3 58 191 73 212 4.32e-20 84.0
Msa0715110 MtrunA17_Chr1g0185001 32.192 146 96 1 50 192 1 146 6.05e-20 82.0
Msa0715110 MtrunA17_Chr1g0166281 35.252 139 83 2 58 192 12 147 2.37e-19 80.5
Msa0715110 MtrunA17_Chr7g0254731 34.532 139 84 2 58 192 12 147 1.49e-18 78.6
Msa0715110 MtrunA17_Chr8g0373691 30.065 153 100 3 46 191 35 187 2.73e-18 78.6
Msa0715110 MtrunA17_Chr5g0440931 34.532 139 84 2 58 192 12 147 7.53e-18 76.6
Msa0715110 MtrunA17_Chr4g0057661 31.618 136 92 1 58 193 6 140 7.84e-18 76.3
Msa0715110 MtrunA17_Chr6g0460211 34.532 139 84 2 58 192 12 147 8.02e-18 76.6
Msa0715110 MtrunA17_Chr4g0057641 31.343 134 92 0 58 191 6 139 2.03e-17 75.1
Msa0715110 MtrunA17_Chr4g0064231 34.320 169 92 3 45 194 51 219 7.14e-17 75.9
Msa0715110 MtrunA17_Chr7g0274101 36.364 132 83 1 63 193 17 148 1.30e-16 73.2
Msa0715110 MtrunA17_Chr4g0057651 31.618 136 92 1 58 193 6 140 1.54e-16 72.8
Msa0715110 MtrunA17_Chr8g0366401 29.412 136 92 1 58 189 17 152 2.61e-16 72.8
Msa0715110 MtrunA17_Chr7g0245231 32.258 155 102 3 40 193 13 165 2.62e-16 73.2
Msa0715110 MtrunA17_Chr6g0450921 32.258 155 102 3 40 193 12 164 3.55e-16 72.8
Msa0715110 MtrunA17_Chr7g0267751 30.882 136 92 1 59 194 441 574 6.39e-15 72.4
Msa0715110 MtrunA17_Chr5g0396261 35.714 98 58 2 97 190 16 112 1.57e-14 67.0
Msa0715110 MtrunA17_Chr4g0062221 26.619 139 97 2 57 191 10 147 1.65e-14 68.2
Msa0715110 MtrunA17_Chr1g0176751 31.387 137 90 2 59 194 430 563 1.88e-14 70.9
Msa0715110 MtrunA17_Chr1g0170281 31.618 136 91 1 59 194 424 557 2.75e-14 70.5
Msa0715110 MtrunA17_Chr1g0157131 30.435 138 91 2 59 194 380 514 3.44e-14 70.1
Msa0715110 MtrunA17_Chr8g0368681 28.188 149 99 4 50 191 1 148 3.51e-14 67.0
Msa0715110 MtrunA17_Chr3g0125311 30.065 153 84 5 58 192 13 160 5.94e-14 66.6
Msa0715110 MtrunA17_Chr5g0441691 30.714 140 96 1 59 198 374 512 6.64e-14 69.3
Msa0715110 MtrunA17_Chr5g0448721 30.882 136 92 1 59 194 402 535 7.67e-14 69.3
Msa0715110 MtrunA17_Chr4g0068501 28.477 151 103 3 46 191 4 154 9.20e-14 66.2
Msa0715110 MtrunA17_Chr5g0444171 30.147 136 94 1 59 194 398 532 1.70e-13 68.2
Msa0715110 MtrunA17_Chr0c02g0489761 30.714 140 96 1 59 198 384 522 2.02e-13 68.2
Msa0715110 MtrunA17_Chr1g0160151 30.769 143 90 4 57 192 21 161 2.10e-13 65.1
Msa0715110 MtrunA17_Chr1g0200601 31.618 136 91 1 59 194 336 469 2.62e-13 67.8
Msa0715110 MtrunA17_Chr1g0173541 33.333 132 88 0 63 194 362 493 4.22e-13 67.0
Msa0715110 MtrunA17_Chr5g0397101 30.435 138 91 2 59 194 372 506 4.27e-13 67.0
Msa0715110 MtrunA17_Chr8g0385341 32.353 136 90 1 59 194 336 469 5.47e-13 66.6
Msa0715110 MtrunA17_Chr4g0074811 29.412 136 95 1 59 194 397 531 7.16e-13 66.2
Msa0715110 MtrunA17_Chr8g0387671 29.710 138 92 2 59 194 372 506 1.90e-12 65.1
Msa0715110 MtrunA17_Chr7g0257751 30.303 132 92 0 63 194 368 499 2.32e-12 65.1
Msa0715110 MtrunA17_Chr7g0242561 28.261 138 99 0 57 194 360 497 2.72e-12 64.7
Msa0715110 MtrunA17_Chr5g0405421 29.787 141 86 3 58 191 406 540 1.78e-11 62.4
Msa0715110 MtrunA17_Chr3g0098951 28.472 144 97 2 59 196 389 532 2.35e-11 62.0
Msa0715110 MtrunA17_Chr3g0131181 30.882 136 92 1 59 194 335 468 2.69e-11 61.6
Msa0715110 MtrunA17_Chr4g0074791 32.353 136 81 2 59 194 401 525 5.50e-11 60.8
Msa0715110 MtrunA17_Chr4g0059951 27.711 166 115 4 32 194 351 514 7.76e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0715110 AT2G15680.1 49.640 139 69 1 53 191 45 182 4.15e-44 145
Msa0715110 AT5G37770.1 41.259 143 79 1 57 194 16 158 5.69e-33 116
Msa0715110 AT5G37770.2 41.259 143 79 1 57 194 16 158 5.69e-33 116
Msa0715110 AT1G24620.1 43.972 141 76 2 58 196 37 176 1.21e-32 116
Msa0715110 AT1G18210.2 37.342 158 94 2 34 191 1 153 8.49e-31 111
Msa0715110 AT1G18210.1 37.342 158 94 2 34 191 1 153 8.49e-31 111
Msa0715110 AT1G66400.1 39.716 141 78 2 57 192 14 152 2.55e-30 109
Msa0715110 AT5G17470.1 43.165 139 75 3 62 196 6 144 4.28e-29 105
Msa0715110 AT3G03400.1 44.000 125 65 2 62 182 9 132 2.88e-28 103
Msa0715110 AT2G36180.1 42.958 142 76 3 59 196 1 141 2.30e-27 101
Msa0715110 AT1G73630.1 37.671 146 87 2 47 191 8 150 3.23e-27 101
Msa0715110 AT5G42380.1 37.956 137 83 1 57 191 48 184 3.75e-27 102
Msa0715110 AT3G50770.1 33.908 174 100 3 24 191 39 203 4.24e-25 97.4
Msa0715110 AT1G76650.1 31.250 176 115 2 16 191 7 176 5.05e-24 94.0
Msa0715110 AT1G76650.3 31.250 176 115 2 16 191 7 176 5.05e-24 94.0
Msa0715110 AT2G43290.1 37.241 145 83 2 58 194 65 209 9.51e-24 94.0
Msa0715110 AT3G59440.1 37.324 142 81 3 58 194 51 189 1.35e-23 93.2
Msa0715110 AT4G14640.1 35.714 140 83 2 58 193 13 149 2.29e-21 86.3
Msa0715110 AT1G05990.1 38.298 141 83 2 58 194 5 145 2.59e-21 85.9
Msa0715110 AT3G50360.1 31.928 166 107 2 27 191 1 161 3.78e-21 86.3
Msa0715110 AT1G76640.1 34.641 153 96 2 43 191 6 158 8.88e-21 84.7
Msa0715110 AT3G07490.1 36.111 144 89 2 58 198 5 148 2.13e-20 83.6
Msa0715110 AT4G14640.2 35.714 126 74 2 72 193 9 131 6.24e-20 82.0
Msa0715110 AT3G22930.1 35.252 139 88 2 58 194 35 173 2.13e-19 81.6
Msa0715110 AT4G03290.1 35.417 144 87 2 58 195 5 148 2.16e-19 81.3
Msa0715110 AT2G41410.1 30.573 157 98 5 46 192 53 208 3.29e-18 79.3
Msa0715110 AT4G12860.1 36.301 146 90 2 56 198 3 148 3.60e-18 77.8
Msa0715110 AT1G76650.2 28.977 176 109 3 16 191 7 166 3.62e-18 78.2
Msa0715110 AT2G27030.3 34.266 143 87 2 58 196 12 151 4.80e-18 78.2
Msa0715110 AT3G43810.2 34.014 147 88 3 58 198 12 155 5.18e-18 79.0
Msa0715110 AT5G21274.1 35.252 139 83 2 58 192 12 147 5.57e-18 77.4
Msa0715110 AT1G66410.1 34.532 139 84 2 58 192 12 147 6.39e-18 77.0
Msa0715110 AT5G37780.1 34.532 139 84 2 58 192 12 147 6.39e-18 77.0
Msa0715110 AT3G56800.1 35.252 139 83 2 58 192 12 147 7.03e-18 77.0
Msa0715110 AT2G41110.1 35.252 139 83 2 58 192 12 147 7.03e-18 77.0
Msa0715110 AT2G27030.1 35.252 139 83 2 58 192 12 147 7.03e-18 77.0
Msa0715110 AT3G43810.1 34.532 139 84 2 58 192 12 147 1.11e-17 76.6
Msa0715110 AT1G32250.1 28.767 146 89 3 57 191 15 156 4.72e-17 75.5
Msa0715110 AT3G03410.1 34.586 133 79 1 60 192 4 128 4.73e-17 74.3
Msa0715110 AT3G10190.1 32.353 136 90 2 58 191 70 205 6.05e-17 75.9
Msa0715110 AT5G04870.1 32.847 137 88 2 59 194 456 589 8.00e-16 75.5
Msa0715110 AT4G04695.1 33.824 136 89 1 59 194 337 471 8.73e-16 75.1
Msa0715110 AT5G44460.1 35.758 165 90 7 42 191 11 174 1.35e-15 71.6
Msa0715110 AT5G37780.4 34.400 125 75 2 72 192 13 134 1.55e-15 70.5
Msa0715110 AT2G17290.1 33.824 136 88 1 59 194 391 524 1.76e-15 74.3
Msa0715110 AT2G17290.2 33.824 136 88 1 59 194 391 524 1.76e-15 74.3
Msa0715110 AT1G66410.2 34.400 125 75 2 72 192 36 157 2.09e-15 70.9
Msa0715110 AT5G23580.1 33.083 133 87 1 59 191 328 458 2.14e-15 73.9
Msa0715110 AT2G41110.2 35.200 125 74 2 72 192 38 159 2.16e-15 70.9
Msa0715110 AT5G37780.3 34.400 125 75 2 72 192 52 173 3.19e-15 70.5
Msa0715110 AT5G37780.2 34.400 125 75 2 72 192 41 162 4.13e-15 70.1
Msa0715110 AT4G37010.2 29.710 138 96 1 55 191 28 165 4.29e-15 70.1
Msa0715110 AT1G66410.4 41.053 95 49 2 102 192 20 111 4.37e-15 68.6
Msa0715110 AT1G66410.3 41.053 95 49 2 102 192 20 111 4.37e-15 68.6
Msa0715110 AT4G37010.1 29.710 138 96 1 55 191 24 161 4.98e-15 70.1
Msa0715110 AT3G43810.4 41.053 95 49 2 102 192 20 111 8.59e-15 67.8
Msa0715110 AT3G43810.3 41.053 95 49 2 102 192 20 111 8.59e-15 67.8
Msa0715110 AT3G10660.2 31.618 136 91 1 59 194 492 625 8.90e-15 72.4
Msa0715110 AT3G10660.1 31.618 136 91 1 59 194 492 625 8.90e-15 72.4
Msa0715110 AT5G19360.1 32.353 136 91 1 59 194 374 508 9.94e-15 72.4
Msa0715110 AT2G41100.7 32.051 156 87 4 58 195 66 220 1.00e-14 71.2
Msa0715110 AT2G41100.6 32.051 156 87 4 58 195 66 220 1.00e-14 71.2
Msa0715110 AT2G41100.3 32.051 156 87 4 58 195 66 220 1.00e-14 71.2
Msa0715110 AT5G12180.1 32.353 136 91 1 59 194 379 513 1.10e-14 72.0
Msa0715110 AT4G38230.1 33.824 136 88 1 59 194 186 319 1.44e-14 71.2
Msa0715110 AT2G41100.4 32.051 156 87 4 58 195 101 255 1.56e-14 70.9
Msa0715110 AT2G41100.4 30.464 151 88 3 58 191 12 162 2.68e-12 64.7
Msa0715110 AT2G41100.1 32.051 156 87 4 58 195 101 255 1.56e-14 70.9
Msa0715110 AT2G41100.1 30.464 151 88 3 58 191 12 162 2.68e-12 64.7
Msa0715110 AT4G04720.2 32.353 136 91 1 59 194 385 519 1.59e-14 71.6
Msa0715110 AT4G04720.1 32.353 136 91 1 59 194 385 519 1.59e-14 71.6
Msa0715110 AT4G38230.2 33.824 136 88 1 59 194 360 493 1.78e-14 71.6
Msa0715110 AT4G38230.4 33.824 136 88 1 59 194 330 463 2.01e-14 71.2
Msa0715110 AT4G38230.3 33.824 136 88 1 59 194 330 463 2.01e-14 71.2
Msa0715110 AT1G18530.1 30.435 138 91 3 58 191 7 143 2.22e-14 68.2
Msa0715110 AT4G09570.1 33.824 136 88 2 59 194 331 464 2.44e-14 71.2
Msa0715110 AT5G12480.2 33.577 137 90 1 59 194 271 407 3.03e-14 70.9
Msa0715110 AT5G12480.1 33.577 137 90 1 59 194 365 501 3.70e-14 70.5
Msa0715110 AT1G35670.1 33.088 136 89 2 59 194 332 465 4.31e-14 70.5
Msa0715110 AT4G04700.1 33.083 133 88 1 59 191 337 468 5.69e-14 70.1
Msa0715110 AT3G03000.1 30.657 137 91 3 58 191 20 155 5.88e-14 67.0
Msa0715110 AT3G51850.1 29.710 138 97 0 57 194 358 495 6.52e-14 69.7
Msa0715110 AT2G38910.1 31.852 135 90 1 59 193 440 572 8.94e-14 69.3
Msa0715110 AT4G04710.3 32.353 136 91 1 59 194 324 458 1.30e-13 68.9
Msa0715110 AT4G04710.2 32.353 136 91 1 59 194 338 472 1.32e-13 68.9
Msa0715110 AT4G04710.1 32.353 136 91 1 59 194 351 485 1.40e-13 68.9
Msa0715110 AT4G35310.2 31.618 136 91 1 59 194 403 536 1.53e-13 68.9
Msa0715110 AT4G35310.1 31.618 136 91 1 59 194 403 536 1.53e-13 68.9
Msa0715110 AT4G23650.1 31.884 138 89 2 59 194 384 518 2.27e-13 68.2
Msa0715110 AT2G41090.1 31.111 135 91 1 58 192 12 144 2.63e-13 65.9
Msa0715110 AT1G21550.1 30.612 147 91 4 57 193 9 154 2.67e-13 65.1
Msa0715110 AT2G17890.1 32.353 136 79 3 63 191 420 549 3.64e-13 67.8
Msa0715110 AT4G04740.4 29.412 136 95 1 59 194 374 508 4.00e-13 67.4
Msa0715110 AT4G04740.6 29.412 136 95 1 59 194 374 508 4.00e-13 67.4
Msa0715110 AT4G04740.5 29.412 136 95 1 59 194 374 508 4.00e-13 67.4
Msa0715110 AT4G04740.3 29.412 136 95 1 59 194 374 508 4.00e-13 67.4
Msa0715110 AT4G04740.1 29.412 136 95 1 59 194 374 508 4.00e-13 67.4
Msa0715110 AT4G04740.7 29.412 136 95 1 59 194 374 508 4.00e-13 67.4
Msa0715110 AT3G25600.1 28.261 138 94 3 58 191 12 148 8.39e-13 63.9
Msa0715110 AT1G50700.1 30.882 136 93 1 59 194 379 513 8.56e-13 66.6
Msa0715110 AT4G36070.1 31.690 142 84 4 57 191 377 512 8.88e-13 66.6
Msa0715110 AT4G36070.2 31.690 142 84 4 57 191 377 512 1.06e-12 66.2
Msa0715110 AT3G57530.1 33.083 133 86 2 63 194 373 503 1.11e-12 66.2
Msa0715110 AT4G21940.2 31.618 136 92 1 59 194 407 541 2.74e-12 65.1
Msa0715110 AT4G21940.1 31.618 136 92 1 59 194 407 541 2.97e-12 65.1
Msa0715110 AT4G04710.4 31.884 138 91 2 59 194 351 487 4.17e-12 64.7
Msa0715110 AT1G18890.1 30.539 167 109 6 32 194 341 504 4.58e-12 64.3
Msa0715110 AT4G20780.1 28.814 177 98 4 36 191 14 183 1.55e-11 61.2
Msa0715110 AT5G19450.2 32.847 137 91 1 59 194 363 499 2.06e-11 62.4
Msa0715110 AT5G19450.1 32.847 137 91 1 59 194 363 499 2.06e-11 62.4
Msa0715110 AT1G61950.1 30.282 142 91 3 59 198 405 540 2.10e-11 62.4
Msa0715110 AT3G20410.2 30.147 136 94 1 59 194 397 531 2.34e-11 62.4
Msa0715110 AT3G20410.1 30.147 136 94 1 59 194 397 531 2.34e-11 62.4
Msa0715110 AT5G66210.1 30.216 139 78 4 63 191 374 503 2.93e-11 62.0
Msa0715110 AT5G66210.2 30.216 139 78 4 63 191 374 503 2.93e-11 62.0
Msa0715110 AT2G41100.2 34.821 112 54 4 102 195 20 130 5.42e-11 59.7
Msa0715110 AT1G76040.2 28.676 136 96 1 59 194 417 551 8.40e-11 60.8

Find 36 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCATTTCATCTTTCTT+TGG 0.177414 5_1:-8001737 None:intergenic
GTCAGTGTCCATGGCTTTAA+AGG 0.198746 5_1:-8001870 None:intergenic
TTTCAAGTATTTGATATAAA+TGG 0.216373 5_1:+8001980 Msa0715110:CDS
CATAGACTTTAAAGAGTTCA+TGG 0.277650 5_1:+8001904 Msa0715110:CDS
TTGAGTTTCCTAAAGTTTCA+TGG 0.320314 5_1:+8001615 Msa0715110:CDS
AAGCTATACCATGAAACTTT+AGG 0.341048 5_1:-8001623 None:intergenic
CCTTAGTGCTTGTAAGAAAA+TGG 0.341545 5_1:+8002066 Msa0715110:CDS
CAAAGAAAGATGAAATGAAA+TGG 0.359646 5_1:+8001738 Msa0715110:CDS
GCTGCAAAAGCTTTGGATAA+GGG 0.402200 5_1:+8001821 Msa0715110:CDS
TAAAGCAGCTGCAAAAGCTT+TGG 0.411228 5_1:+8001814 Msa0715110:CDS
AATTGTCTCAAATATTTAAG+AGG 0.413219 5_1:+8002026 Msa0715110:CDS
TCTCAAATATTTAAGAGGTT+AGG 0.421604 5_1:+8002031 Msa0715110:CDS
TCATTTCATCTTTCTTTGGT+TGG 0.424902 5_1:-8001733 None:intergenic
GCCATCTTTGTCAGTGTCCA+TGG 0.434255 5_1:-8001879 None:intergenic
AGCTGCAAAAGCTTTGGATA+AGG 0.434736 5_1:+8001820 Msa0715110:CDS
CAAAGATGGCAAGATTAGTC+TGG 0.440035 5_1:+8001784 Msa0715110:CDS
AAAGAAAGATGAAATGAAAT+GGG 0.477872 5_1:+8001739 Msa0715110:CDS
TAGATTTGAATGAGTTTACA+AGG 0.501078 5_1:+8002122 Msa0715110:CDS
AAGCTTTGGATAAGGGGATA+GGG 0.522510 5_1:+8001828 Msa0715110:CDS
CTGCAAAAGCTTTGGATAAG+GGG 0.525087 5_1:+8001822 Msa0715110:CDS
AAAATGGTGAAAGGTGTTGA+TGG 0.531030 5_1:+8002082 Msa0715110:CDS
AAAGCTTTGGATAAGGGGAT+AGG 0.538133 5_1:+8001827 Msa0715110:CDS
GTATTTGATATAAATGGTGA+TGG 0.547891 5_1:+8001986 Msa0715110:CDS
GAGTTCATGGAAATGTTCAA+TGG 0.548455 5_1:+8001917 Msa0715110:CDS
ATGGAAATGTTCAATGGAGA+AGG 0.556462 5_1:+8001923 Msa0715110:CDS
AGGTAGTAAGATAAAAGAAG+AGG 0.565885 5_1:+8001943 Msa0715110:CDS
GGTGTTGATGGTGATGGAGA+TGG 0.571699 5_1:+8002094 Msa0715110:CDS
GTGAAAGGTGTTGATGGTGA+TGG 0.580268 5_1:+8002088 Msa0715110:CDS
AGCTTTGGATAAGGGGATAG+GGG 0.581892 5_1:+8001829 Msa0715110:CDS
AAATTCGAGACAAACAAAGA+TGG 0.598029 5_1:+8001770 Msa0715110:CDS
TTTACAAGGATGATGATGAA+TGG 0.601621 5_1:+8002136 Msa0715110:CDS
TATTTGATATAAATGGTGAT+GGG 0.605312 5_1:+8001987 Msa0715110:CDS
GCCATGGACACTGACAAAGA+TGG 0.618305 5_1:+8001878 Msa0715110:CDS
AGTGAAAGCCTTTAAAGCCA+TGG 0.624659 5_1:+8001862 Msa0715110:CDS
GCTTGTAAGAAAATGGTGAA+AGG 0.660596 5_1:+8002073 Msa0715110:CDS
TGATGGGAAAATCAGTGCTG+AGG 0.731787 5_1:+8002003 Msa0715110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAAGTATTTGATATAAA+TGG + chr5_1:8001980-8001999 Msa0715110:CDS 15.0%
!! AAAGAAAGATGAAATGAAAT+GGG + chr5_1:8001739-8001758 Msa0715110:CDS 20.0%
!! TATTTGATATAAATGGTGAT+GGG + chr5_1:8001987-8002006 Msa0715110:CDS 20.0%
!! AATTGTCTCAAATATTTAAG+AGG + chr5_1:8002026-8002045 Msa0715110:CDS 20.0%
!!! TTTTGAGCTATATTTTTTCG+TGG - chr5_1:8001668-8001687 None:intergenic 25.0%
! CATTTCATTTCATCTTTCTT+TGG - chr5_1:8001740-8001759 None:intergenic 25.0%
! CAAAGAAAGATGAAATGAAA+TGG + chr5_1:8001738-8001757 Msa0715110:CDS 25.0%
! GTATTTGATATAAATGGTGA+TGG + chr5_1:8001986-8002005 Msa0715110:CDS 25.0%
! TCTCAAATATTTAAGAGGTT+AGG + chr5_1:8002031-8002050 Msa0715110:CDS 25.0%
! TAGATTTGAATGAGTTTACA+AGG + chr5_1:8002122-8002141 Msa0715110:CDS 25.0%
!! TAGGAAACTCAATTTTGACA+TGG - chr5_1:8001607-8001626 None:intergenic 30.0%
TTGAGTTTCCTAAAGTTTCA+TGG + chr5_1:8001615-8001634 Msa0715110:CDS 30.0%
AAGCTATACCATGAAACTTT+AGG - chr5_1:8001626-8001645 None:intergenic 30.0%
TCATTTCATCTTTCTTTGGT+TGG - chr5_1:8001736-8001755 None:intergenic 30.0%
AAATTCGAGACAAACAAAGA+TGG + chr5_1:8001770-8001789 Msa0715110:CDS 30.0%
! CATAGACTTTAAAGAGTTCA+TGG + chr5_1:8001904-8001923 Msa0715110:CDS 30.0%
AGGTAGTAAGATAAAAGAAG+AGG + chr5_1:8001943-8001962 Msa0715110:CDS 30.0%
! TTTACAAGGATGATGATGAA+TGG + chr5_1:8002136-8002155 Msa0715110:CDS 30.0%
!!! AGCTATATTTTTTCGTGGAG+AGG - chr5_1:8001663-8001682 None:intergenic 35.0%
GAGTTCATGGAAATGTTCAA+TGG + chr5_1:8001917-8001936 Msa0715110:CDS 35.0%
ATGGAAATGTTCAATGGAGA+AGG + chr5_1:8001923-8001942 Msa0715110:CDS 35.0%
! CCATTTTCTTACAAGCACTA+AGG - chr5_1:8002069-8002088 None:intergenic 35.0%
CCTTAGTGCTTGTAAGAAAA+TGG + chr5_1:8002066-8002085 Msa0715110:CDS 35.0%
GCTTGTAAGAAAATGGTGAA+AGG + chr5_1:8002073-8002092 Msa0715110:CDS 35.0%
!! AAAATGGTGAAAGGTGTTGA+TGG + chr5_1:8002082-8002101 Msa0715110:CDS 35.0%
! CAAAGATGGCAAGATTAGTC+TGG + chr5_1:8001784-8001803 Msa0715110:CDS 40.0%
TAAAGCAGCTGCAAAAGCTT+TGG + chr5_1:8001814-8001833 Msa0715110:CDS 40.0%
!! AGCTGCAAAAGCTTTGGATA+AGG + chr5_1:8001820-8001839 Msa0715110:CDS 40.0%
!! GCTGCAAAAGCTTTGGATAA+GGG + chr5_1:8001821-8001840 Msa0715110:CDS 40.0%
!! CTGCAAAAGCTTTGGATAAG+GGG + chr5_1:8001822-8001841 Msa0715110:CDS 40.0%
!! AAAGCTTTGGATAAGGGGAT+AGG + chr5_1:8001827-8001846 Msa0715110:CDS 40.0%
!! AAGCTTTGGATAAGGGGATA+GGG + chr5_1:8001828-8001847 Msa0715110:CDS 40.0%
!! AGTGAAAGCCTTTAAAGCCA+TGG + chr5_1:8001862-8001881 Msa0715110:CDS 40.0%
!! TTTTCGTGGAGAGGTTAGCT+TGG - chr5_1:8001654-8001673 None:intergenic 45.0%
!! AGCTTTGGATAAGGGGATAG+GGG + chr5_1:8001829-8001848 Msa0715110:CDS 45.0%
!! GTCAGTGTCCATGGCTTTAA+AGG - chr5_1:8001873-8001892 None:intergenic 45.0%
TGATGGGAAAATCAGTGCTG+AGG + chr5_1:8002003-8002022 Msa0715110:CDS 45.0%
!! GTGAAAGGTGTTGATGGTGA+TGG + chr5_1:8002088-8002107 Msa0715110:CDS 45.0%
GCCATGGACACTGACAAAGA+TGG + chr5_1:8001878-8001897 Msa0715110:CDS 50.0%
GCCATCTTTGTCAGTGTCCA+TGG - chr5_1:8001882-8001901 None:intergenic 50.0%
!! GGTGTTGATGGTGATGGAGA+TGG + chr5_1:8002094-8002113 Msa0715110:CDS 50.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 8001578 8002174 8001578 ID=Msa0715110;Name=Msa0715110
chr5_1 mRNA 8001578 8002174 8001578 ID=Msa0715110-mRNA-1;Parent=Msa0715110;Name=Msa0715110-mRNA-1;_AED=0.03;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|198
chr5_1 exon 8001578 8002174 8001578 ID=Msa0715110-mRNA-1:exon:3320;Parent=Msa0715110-mRNA-1
chr5_1 CDS 8001578 8002174 8001578 ID=Msa0715110-mRNA-1:cds;Parent=Msa0715110-mRNA-1
Gene Sequence

>Msa0715110

ATGGTATCAAAAGTTCTAAAAAAATACCATGTCAAAATTGAGTTTCCTAAAGTTTCATGGTATAGCTTTTCAGCCAAGCTAACCTCTCCACGAAAAAATATAGCTCAAAAGAACAGCTTCAAGTCGAGACAAACATCGAGTACTGAAATGAGTTTCCAACCAAAGAAAGATGAAATGAAATGGGTGTTTGAGAAATTCGAGACAAACAAAGATGGCAAGATTAGTCTGGAAGAGTATAAAGCAGCTGCAAAAGCTTTGGATAAGGGGATAGGGGATACTGATGCAGTGAAAGCCTTTAAAGCCATGGACACTGACAAAGATGGCTTCATAGACTTTAAAGAGTTCATGGAAATGTTCAATGGAGAAGGTAGTAAGATAAAAGAAGAGGAGATTAAGAGTGCTTTTCAAGTATTTGATATAAATGGTGATGGGAAAATCAGTGCTGAGGAATTGTCTCAAATATTTAAGAGGTTAGGAGAGAGTTGTAGCCTTAGTGCTTGTAAGAAAATGGTGAAAGGTGTTGATGGTGATGGAGATGGTCTTATAGATTTGAATGAGTTTACAAGGATGATGATGAATGGCAAGAAATGTGCCTAA
Protein sequence

>Msa0715110

MVSKVLKKYHVKIEFPKVSWYSFSAKLTSPRKNIAQKNSFKSRQTSSTEMSFQPKKDEMKWVFEKFETNKDGKISLEEYKAAAKALDKGIGDTDAVKAFKAMDTDKDGFIDFKEFMEMFNGEGSKIKEEEIKSAFQVFDINGDGKISAEELSQIFKRLGESCSLSACKKMVKGVDGDGDGLIDLNEFTRMMMNGKKCA*