Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0759480 | AES95383.1 | 97.333 | 150 | 3 | 1 | 1 | 150 | 1 | 149 | 2.52e-99 | 292 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0759480 | G7K2M0 | 97.333 | 150 | 3 | 1 | 1 | 150 | 1 | 149 | 1.21e-99 | 292 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0733010 | Msa0759480 | 0.814829 | 1.261920e-51 | -8.615850e-47 |
| Msa0743930 | Msa0759480 | 0.807694 | 4.460780e-50 | -8.615850e-47 |
| Msa0759480 | Msa0759490 | 0.988738 | 3.278825e-175 | -8.615850e-47 |
| Msa0759480 | Msa0846860 | 0.806648 | 7.428326e-50 | -8.615850e-47 |
| Msa0759480 | Msa0855870 | 0.807964 | 3.907364e-50 | -8.615850e-47 |
| Msa0759480 | Msa0899970 | 0.801141 | 1.035803e-48 | -8.615850e-47 |
| Msa0759480 | Msa0965080 | 0.811605 | 6.439426e-51 | -8.615850e-47 |
| Msa0759480 | Msa0965290 | 0.819840 | 9.402158e-53 | -8.615850e-47 |
| Msa0759480 | Msa0999680 | 0.817473 | 3.238820e-52 | -8.615850e-47 |
| Msa0759480 | Msa1423780 | 0.809107 | 2.227646e-50 | -8.615850e-47 |
| Msa0230320 | Msa0759480 | 0.805019 | 1.633641e-49 | -8.615850e-47 |
| Msa0432160 | Msa0759480 | 0.814125 | 1.806945e-51 | -8.615850e-47 |
| Msa0476680 | Msa0759480 | 0.813921 | 2.003255e-51 | -8.615850e-47 |
| Msa0240900 | Msa0759480 | 0.802571 | 5.266963e-49 | -8.615850e-47 |
| Msa0308630 | Msa0759480 | 0.800684 | 1.284258e-48 | -8.615850e-47 |
| Msa0317970 | Msa0759480 | 0.809040 | 2.302518e-50 | -8.615850e-47 |
| Msa0521010 | Msa0759480 | 0.815821 | 7.594116e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0759480 | MtrunA17_Chr5g0407551 | 97.333 | 150 | 3 | 1 | 1 | 150 | 1 | 149 | 2.32e-103 | 292 |
| Msa0759480 | MtrunA17_Chr8g0369991 | 61.871 | 139 | 37 | 3 | 1 | 138 | 1 | 124 | 2.21e-48 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAACATCTATTTCTTCTA+TGG | 0.231886 | 5_2:-15222233 | Msa0759480:CDS |
| TCATGAATATGTACTACAAT+TGG | 0.324853 | 5_2:+15222196 | None:intergenic |
| TAACTCTGTTGTAACTCATT+TGG | 0.334667 | 5_2:+15222605 | None:intergenic |
| ATGGAAAATGCAGAGGATTT+AGG | 0.352171 | 5_2:-15222540 | Msa0759480:CDS |
| AGAAGCTATTGCAGATTGTT+TGG | 0.378114 | 5_2:-15222266 | Msa0759480:CDS |
| AATAGCAATAGTAGTAGAAA+TGG | 0.412385 | 5_2:-15222370 | Msa0759480:CDS |
| CATGAATATGTACTACAATT+GGG | 0.470108 | 5_2:+15222197 | None:intergenic |
| ACCAGCAGCAGTAGTGGTAA+AGG | 0.475843 | 5_2:-15222577 | Msa0759480:CDS |
| TCCTTTACCACTACTGCTGC+TGG | 0.481982 | 5_2:+15222576 | None:intergenic |
| AATGAAGGATGAAAGAATCA+AGG | 0.492289 | 5_2:-15222344 | Msa0759480:CDS |
| GAAAGAATCAAGGGTCAAGC+AGG | 0.515043 | 5_2:-15222334 | Msa0759480:CDS |
| AGCAGCACCAGCAGCAGTAG+TGG | 0.516312 | 5_2:-15222583 | Msa0759480:CDS |
| TAGTAGAAATGGTTTAATGA+AGG | 0.548221 | 5_2:-15222359 | Msa0759480:CDS |
| TCAACTTATCAAAGATTCTG+AGG | 0.566527 | 5_2:+15222464 | None:intergenic |
| ATGAAGGATGAAAGAATCAA+GGG | 0.587644 | 5_2:-15222343 | Msa0759480:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAGCAATAGTAGTAGAAA+TGG | - | chr5_2:15222420-15222439 | Msa0759480:CDS | 25.0% |
| ! | TAGTAGAAATGGTTTAATGA+AGG | - | chr5_2:15222431-15222450 | Msa0759480:CDS | 25.0% |
| !! | GATTGTAGATTTTCATCTTA+TGG | - | chr5_2:15222480-15222499 | Msa0759480:CDS | 25.0% |
| !! | AAGAACATCTATTTCTTCTA+TGG | - | chr5_2:15222557-15222576 | Msa0759480:CDS | 25.0% |
| ! | CATGAATATGTACTACAATT+GGG | + | chr5_2:15222596-15222615 | None:intergenic | 25.0% |
| ! | TCATGAATATGTACTACAAT+TGG | + | chr5_2:15222597-15222616 | None:intergenic | 25.0% |
| TAACTCTGTTGTAACTCATT+TGG | + | chr5_2:15222188-15222207 | None:intergenic | 30.0% | |
| ! | AAAGGATCATCATTTTACCA+TGG | - | chr5_2:15222231-15222250 | Msa0759480:CDS | 30.0% |
| !!! | TCACAAGACTTTACTTTTTG+CGG | - | chr5_2:15222280-15222299 | Msa0759480:CDS | 30.0% |
| TCAACTTATCAAAGATTCTG+AGG | + | chr5_2:15222329-15222348 | None:intergenic | 30.0% | |
| ! | AAAGTTTTCTGCAGAAAAAG+TGG | - | chr5_2:15222384-15222403 | Msa0759480:CDS | 30.0% |
| AATGAAGGATGAAAGAATCA+AGG | - | chr5_2:15222446-15222465 | Msa0759480:CDS | 30.0% | |
| ATGAAGGATGAAAGAATCAA+GGG | - | chr5_2:15222447-15222466 | Msa0759480:CDS | 30.0% | |
| ! | GTAGATTTTCATCTTATGGA+AGG | - | chr5_2:15222484-15222503 | Msa0759480:CDS | 30.0% |
| ! | TTTTACCATGGAAAATGCAG+AGG | - | chr5_2:15222243-15222262 | Msa0759480:CDS | 35.0% |
| ! | TAAATCCTCTGCATTTTCCA+TGG | + | chr5_2:15222251-15222270 | None:intergenic | 35.0% |
| ATGGAAAATGCAGAGGATTT+AGG | - | chr5_2:15222250-15222269 | Msa0759480:CDS | 35.0% | |
| ! | GCAAAAAGTAAAGTCTTGTG+AGG | + | chr5_2:15222281-15222300 | None:intergenic | 35.0% |
| AGAAGCTATTGCAGATTGTT+TGG | - | chr5_2:15222524-15222543 | Msa0759480:CDS | 35.0% | |
| GAAAGAATCAAGGGTCAAGC+AGG | - | chr5_2:15222456-15222475 | Msa0759480:CDS | 45.0% | |
| ACCAGCAGCAGTAGTGGTAA+AGG | - | chr5_2:15222213-15222232 | Msa0759480:CDS | 50.0% | |
| TCCTTTACCACTACTGCTGC+TGG | + | chr5_2:15222217-15222236 | None:intergenic | 50.0% | |
| AGCAGCACCAGCAGCAGTAG+TGG | - | chr5_2:15222207-15222226 | Msa0759480:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 15222180 | 15222632 | 15222180 | ID=Msa0759480;Name=Msa0759480 |
| chr5_2 | mRNA | 15222180 | 15222632 | 15222180 | ID=Msa0759480-mRNA-1;Parent=Msa0759480;Name=Msa0759480-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|150 |
| chr5_2 | exon | 15222180 | 15222632 | 15222180 | ID=Msa0759480-mRNA-1:exon:6913;Parent=Msa0759480-mRNA-1 |
| chr5_2 | CDS | 15222180 | 15222632 | 15222180 | ID=Msa0759480-mRNA-1:cds;Parent=Msa0759480-mRNA-1 |
| Gene Sequence |
| Protein sequence |
