Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0759490 | AES95383.1 | 97.333 | 150 | 3 | 1 | 1 | 150 | 1 | 149 | 2.52e-99 | 292 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0759490 | G7K2M0 | 97.333 | 150 | 3 | 1 | 1 | 150 | 1 | 149 | 1.21e-99 | 292 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0733010 | Msa0759490 | 0.803912 | 2.779889e-49 | -8.615850e-47 |
| Msa0743930 | Msa0759490 | 0.809962 | 1.459564e-50 | -8.615850e-47 |
| Msa0759480 | Msa0759490 | 0.988738 | 3.278825e-175 | -8.615850e-47 |
| Msa0759490 | Msa0816130 | 0.801069 | 1.071187e-48 | -8.615850e-47 |
| Msa0759490 | Msa0846860 | 0.810257 | 1.260944e-50 | -8.615850e-47 |
| Msa0759490 | Msa0855870 | 0.807725 | 4.393930e-50 | -8.615850e-47 |
| Msa0759490 | Msa0899970 | 0.802965 | 4.368350e-49 | -8.615850e-47 |
| Msa0759490 | Msa0921420 | 0.801782 | 7.653995e-49 | -8.615850e-47 |
| Msa0759490 | Msa0965080 | 0.812851 | 3.442479e-51 | -8.615850e-47 |
| Msa0759490 | Msa0965290 | 0.820098 | 8.208185e-53 | -8.615850e-47 |
| Msa0759490 | Msa0999680 | 0.817567 | 3.083376e-52 | -8.615850e-47 |
| Msa0759490 | Msa1012800 | 0.804772 | 1.839904e-49 | -8.615850e-47 |
| Msa0759490 | Msa1259860 | 0.811040 | 8.540597e-51 | -8.615850e-47 |
| Msa0759490 | Msa1369920 | -0.800175 | 1.630330e-48 | -8.615850e-47 |
| Msa0759490 | Msa1423780 | 0.812961 | 3.256997e-51 | -8.615850e-47 |
| Msa0202800 | Msa0759490 | 0.800333 | 1.514153e-48 | -8.615850e-47 |
| Msa0230320 | Msa0759490 | 0.808048 | 3.749853e-50 | -8.615850e-47 |
| Msa0382370 | Msa0759490 | 0.806152 | 9.448250e-50 | -8.615850e-47 |
| Msa0432160 | Msa0759490 | 0.818993 | 1.466695e-52 | -8.615850e-47 |
| Msa0476680 | Msa0759490 | 0.817129 | 3.870103e-52 | -8.615850e-47 |
| Msa0240900 | Msa0759490 | 0.801050 | 1.080994e-48 | -8.615850e-47 |
| Msa0308630 | Msa0759490 | 0.804689 | 1.915248e-49 | -8.615850e-47 |
| Msa0317970 | Msa0759490 | 0.808556 | 2.922854e-50 | -8.615850e-47 |
| Msa0521010 | Msa0759490 | 0.819704 | 1.009876e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0759490 | MtrunA17_Chr5g0407551 | 97.333 | 150 | 3 | 1 | 1 | 150 | 1 | 149 | 2.32e-103 | 292 |
| Msa0759490 | MtrunA17_Chr8g0369991 | 61.871 | 139 | 37 | 3 | 1 | 138 | 1 | 124 | 2.21e-48 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAACATCTATTTCTTCTA+TGG | 0.231886 | 5_2:-15224733 | Msa0759490:CDS |
| TCATGAATATGTACTACAAT+TGG | 0.324853 | 5_2:+15224696 | None:intergenic |
| TAACTCTGTTGTAACTCATT+TGG | 0.334667 | 5_2:+15225105 | None:intergenic |
| ATGGAAAATGCAGAGGATTT+AGG | 0.352171 | 5_2:-15225040 | Msa0759490:CDS |
| AGAAGCTATTGCAGATTGTT+TGG | 0.378114 | 5_2:-15224766 | Msa0759490:CDS |
| AATAGCAATAGTAGTAGAAA+TGG | 0.412385 | 5_2:-15224870 | Msa0759490:CDS |
| CATGAATATGTACTACAATT+GGG | 0.470108 | 5_2:+15224697 | None:intergenic |
| ACCAGCAGCAGTAGTGGTAA+AGG | 0.475843 | 5_2:-15225077 | Msa0759490:CDS |
| TCCTTTACCACTACTGCTGC+TGG | 0.481982 | 5_2:+15225076 | None:intergenic |
| AATGAAGGATGAAAGAATCA+AGG | 0.492289 | 5_2:-15224844 | Msa0759490:CDS |
| GAAAGAATCAAGGGTCAAGC+AGG | 0.515043 | 5_2:-15224834 | Msa0759490:CDS |
| AGCAGCACCAGCAGCAGTAG+TGG | 0.516312 | 5_2:-15225083 | Msa0759490:CDS |
| TAGTAGAAATGGTTTAATGA+AGG | 0.548221 | 5_2:-15224859 | Msa0759490:CDS |
| TCAACTTATCAAAGATTCTG+AGG | 0.566527 | 5_2:+15224964 | None:intergenic |
| ATGAAGGATGAAAGAATCAA+GGG | 0.587644 | 5_2:-15224843 | Msa0759490:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAGCAATAGTAGTAGAAA+TGG | - | chr5_2:15224920-15224939 | Msa0759490:CDS | 25.0% |
| ! | TAGTAGAAATGGTTTAATGA+AGG | - | chr5_2:15224931-15224950 | Msa0759490:CDS | 25.0% |
| !! | GATTGTAGATTTTCATCTTA+TGG | - | chr5_2:15224980-15224999 | Msa0759490:CDS | 25.0% |
| !! | AAGAACATCTATTTCTTCTA+TGG | - | chr5_2:15225057-15225076 | Msa0759490:CDS | 25.0% |
| ! | CATGAATATGTACTACAATT+GGG | + | chr5_2:15225096-15225115 | None:intergenic | 25.0% |
| ! | TCATGAATATGTACTACAAT+TGG | + | chr5_2:15225097-15225116 | None:intergenic | 25.0% |
| TAACTCTGTTGTAACTCATT+TGG | + | chr5_2:15224688-15224707 | None:intergenic | 30.0% | |
| ! | AAAGGATCATCATTTTACCA+TGG | - | chr5_2:15224731-15224750 | Msa0759490:CDS | 30.0% |
| !!! | TCACAAGACTTTACTTTTTG+CGG | - | chr5_2:15224780-15224799 | Msa0759490:CDS | 30.0% |
| TCAACTTATCAAAGATTCTG+AGG | + | chr5_2:15224829-15224848 | None:intergenic | 30.0% | |
| ! | AAAGTTTTCTGCAGAAAAAG+TGG | - | chr5_2:15224884-15224903 | Msa0759490:CDS | 30.0% |
| AATGAAGGATGAAAGAATCA+AGG | - | chr5_2:15224946-15224965 | Msa0759490:CDS | 30.0% | |
| ATGAAGGATGAAAGAATCAA+GGG | - | chr5_2:15224947-15224966 | Msa0759490:CDS | 30.0% | |
| ! | GTAGATTTTCATCTTATGGA+AGG | - | chr5_2:15224984-15225003 | Msa0759490:CDS | 30.0% |
| ! | TTTTACCATGGAAAATGCAG+AGG | - | chr5_2:15224743-15224762 | Msa0759490:CDS | 35.0% |
| ! | TAAATCCTCTGCATTTTCCA+TGG | + | chr5_2:15224751-15224770 | None:intergenic | 35.0% |
| ATGGAAAATGCAGAGGATTT+AGG | - | chr5_2:15224750-15224769 | Msa0759490:CDS | 35.0% | |
| ! | GCAAAAAGTAAAGTCTTGTG+AGG | + | chr5_2:15224781-15224800 | None:intergenic | 35.0% |
| AGAAGCTATTGCAGATTGTT+TGG | - | chr5_2:15225024-15225043 | Msa0759490:CDS | 35.0% | |
| GAAAGAATCAAGGGTCAAGC+AGG | - | chr5_2:15224956-15224975 | Msa0759490:CDS | 45.0% | |
| ACCAGCAGCAGTAGTGGTAA+AGG | - | chr5_2:15224713-15224732 | Msa0759490:CDS | 50.0% | |
| TCCTTTACCACTACTGCTGC+TGG | + | chr5_2:15224717-15224736 | None:intergenic | 50.0% | |
| AGCAGCACCAGCAGCAGTAG+TGG | - | chr5_2:15224707-15224726 | Msa0759490:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 15224680 | 15225132 | 15224680 | ID=Msa0759490;Name=Msa0759490 |
| chr5_2 | mRNA | 15224680 | 15225132 | 15224680 | ID=Msa0759490-mRNA-1;Parent=Msa0759490;Name=Msa0759490-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|150 |
| chr5_2 | exon | 15224680 | 15225132 | 15224680 | ID=Msa0759490-mRNA-1:exon:6914;Parent=Msa0759490-mRNA-1 |
| chr5_2 | CDS | 15224680 | 15225132 | 15224680 | ID=Msa0759490-mRNA-1:cds;Parent=Msa0759490-mRNA-1 |
| Gene Sequence |
| Protein sequence |
