AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0765090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0765090 MtrunA17_Chr5g0415771 98.425 127 2 0 1 127 1 127 3.08e-90 257
Msa0765090 MtrunA17_Chr5g0415761 72.441 127 34 1 1 126 1 127 8.89e-55 167
Msa0765090 MtrunA17_Chr5g0415741 66.667 126 41 1 1 126 1 125 4.97e-53 163
Msa0765090 MtrunA17_Chr7g0216481 60.577 104 41 0 23 126 30 133 6.74e-45 142
Msa0765090 MtrunA17_Chr3g0142051 49.550 111 56 0 15 125 2 112 3.66e-38 125
Msa0765090 MtrunA17_Chr1g0155721 43.750 112 63 0 15 126 2 113 1.04e-34 116
Msa0765090 MtrunA17_Chr5g0404831 44.144 111 62 0 15 125 4 114 5.92e-34 114
Msa0765090 MtrunA17_Chr8g0393201 43.137 102 58 0 21 122 229 330 3.91e-27 102
Msa0765090 MtrunA17_Chr4g0042051 43.396 106 58 2 19 123 10 114 2.07e-25 93.2
Msa0765090 MtrunA17_Chr2g0280411 40.952 105 62 0 19 123 9 113 3.81e-25 92.4
Msa0765090 MtrunA17_Chr7g0245141 39.560 91 55 0 30 120 35 125 6.65e-23 87.0
Msa0765090 MtrunA17_Chr8g0334481 38.710 93 57 0 30 122 37 129 6.65e-23 87.0
Msa0765090 MtrunA17_Chr2g0319291 34.286 105 69 0 19 123 10 114 9.60e-23 86.3
Msa0765090 MtrunA17_Chr1g0201781 39.535 86 49 2 40 124 77 160 3.27e-20 80.9
Msa0765090 MtrunA17_Chr4g0052481 37.805 82 49 1 45 124 85 166 3.42e-18 75.9
Msa0765090 MtrunA17_Chr7g0249461 41.379 87 50 1 41 126 98 184 5.54e-16 70.5
Msa0765090 MtrunA17_Chr3g0125821 37.349 83 50 2 44 124 89 171 6.31e-15 67.4
Msa0765090 MtrunA17_Chr2g0317731 38.554 83 49 2 44 124 89 171 5.05e-14 65.1
Msa0765090 MtrunA17_Chr8g0360031 36.145 83 51 2 44 124 92 174 2.04e-13 63.5
Msa0765090 MtrunA17_Chr1g0211871 34.615 78 47 2 40 116 85 159 4.77e-13 62.4
Msa0765090 MtrunA17_Chr4g0047431 32.500 80 54 0 45 124 25 104 8.24e-12 60.8
Msa0765090 MtrunA17_Chr4g0044731 31.579 76 51 1 44 118 84 159 5.87e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0765090 AT5G39950.1 52.252 111 53 0 16 126 22 132 2.05e-44 141
Msa0765090 AT1G59730.1 43.077 130 70 2 1 127 1 129 1.26e-37 124
Msa0765090 AT3G51030.1 43.243 111 63 0 15 125 2 112 2.11e-33 113
Msa0765090 AT5G42980.1 48.113 106 55 0 21 126 7 112 3.52e-33 112
Msa0765090 AT1G69880.1 48.077 104 54 0 21 124 39 142 5.25e-32 110
Msa0765090 AT1G19730.1 45.536 112 60 1 15 125 2 113 4.31e-31 107
Msa0765090 AT1G45145.1 41.905 105 61 0 21 125 7 111 2.51e-30 105
Msa0765090 AT3G17880.2 39.474 114 65 1 13 122 253 366 3.68e-25 98.2
Msa0765090 AT3G17880.1 39.474 114 65 1 13 122 260 373 4.17e-25 98.2
Msa0765090 AT3G56420.1 39.130 92 56 0 29 120 50 141 7.25e-24 90.1
Msa0765090 AT3G56420.2 39.130 92 56 0 29 120 50 141 7.25e-24 90.1
Msa0765090 AT3G56420.3 39.130 92 56 0 29 120 50 141 7.25e-24 90.1
Msa0765090 AT1G11530.1 38.835 103 63 0 21 123 4 106 3.85e-23 87.4
Msa0765090 AT3G08710.2 40.659 91 54 0 30 120 34 124 8.82e-23 87.0
Msa0765090 AT3G08710.3 40.659 91 54 0 30 120 34 124 8.82e-23 87.0
Msa0765090 AT3G08710.1 40.659 91 54 0 30 120 34 124 8.82e-23 87.0
Msa0765090 AT1G43560.1 42.529 87 48 2 40 124 75 161 7.69e-20 80.1
Msa0765090 AT2G40790.2 39.130 92 56 0 29 120 50 141 9.96e-20 79.7
Msa0765090 AT2G40790.1 39.130 92 56 0 29 120 50 141 9.96e-20 79.7
Msa0765090 AT1G76760.1 39.535 86 50 2 40 123 80 165 2.83e-19 79.0
Msa0765090 AT5G16400.1 42.529 87 49 1 41 126 97 183 1.71e-17 74.7
Msa0765090 AT3G02730.1 43.023 86 48 1 42 126 88 173 9.11e-17 72.4
Msa0765090 AT2G35010.2 39.024 82 48 1 45 124 110 191 2.43e-16 71.6
Msa0765090 AT2G35010.1 39.024 82 48 1 45 124 110 191 2.43e-16 71.6
Msa0765090 AT3G15360.1 33.663 101 62 3 26 124 92 189 8.59e-16 70.5
Msa0765090 AT1G31020.1 39.024 82 48 1 45 124 75 156 8.78e-15 67.0
Msa0765090 AT1G03680.1 38.272 81 48 2 46 124 97 177 1.36e-14 67.0
Msa0765090 AT4G03520.1 33.735 83 53 2 44 124 101 183 1.15e-13 64.7
Msa0765090 AT1G52990.1 34.066 91 58 2 35 124 222 311 2.24e-13 65.5
Msa0765090 AT1G50320.1 39.189 74 41 2 44 116 90 160 7.84e-13 62.4
Msa0765090 AT4G04950.1 29.787 94 66 0 31 124 11 104 8.75e-12 61.2
Msa0765090 AT4G32580.1 30.864 81 56 0 44 124 24 104 8.86e-12 59.3
Msa0765090 AT1G07700.1 36.364 99 55 2 34 124 100 198 1.78e-11 59.3
Msa0765090 AT1G07700.4 36.364 99 55 2 34 124 100 198 1.78e-11 59.3
Msa0765090 AT1G07700.2 36.364 99 55 2 34 124 100 198 1.78e-11 59.3
Msa0765090 AT1G07700.3 36.364 99 55 2 34 124 113 211 3.96e-11 58.5

Find 43 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCTAATTCTTTGATAAT+TGG 0.122091 5_2:+25571097 None:intergenic
CTCAACAACAGACTCTAAAT+TGG 0.230357 5_2:+25571805 None:intergenic
TTGCTTAAGAATCAACATTA+TGG 0.250959 5_2:-25571840 Msa0765090:exon
GGAGGTGGCAAATGCATTTC+AGG 0.257433 5_2:-25570930 Msa0765090:CDS
GATTGACGTGGATGAGTTAA+TGG 0.268688 5_2:-25571047 Msa0765090:intron
CCTTCCACTCAACTGCTAAA+TGG 0.287365 5_2:-25571754 Msa0765090:CDS
CAGCTAATTCTTTGATAATT+GGG 0.312442 5_2:+25571098 None:intergenic
TGTTGTGTAGGACCTTATAT+AGG 0.360903 5_2:+25571916 None:intergenic
TGCTTAAGAATCAACATTAT+GGG 0.362358 5_2:-25571839 Msa0765090:exon
GAAAGGAAAAGTAGTTGAAA+AGG 0.382961 5_2:-25570876 Msa0765090:CDS
TACCAGCTTGTTTGTTACTT+TGG 0.383274 5_2:+25571712 None:intergenic
AAAGGTAGTGGGAGCCAAAA+AGG 0.392388 5_2:-25570858 Msa0765090:CDS
TATCCATGCATGTGTACTAT+AGG 0.411145 5_2:-25570578 Msa0765090:three_prime_UTR
AGACTCTAAATTGGAAAAGT+TGG 0.412160 5_2:+25571814 None:intergenic
ACTTAATTAGTACTTGGTAT+TGG 0.421113 5_2:+25570713 None:intergenic
AAAGATGGTCATTGATTTCA+CGG 0.422756 5_2:-25571152 Msa0765090:intron
ATACGTAGTCACCCTATATA+AGG 0.437300 5_2:-25571928 None:intergenic
AAAAGTAGTTGAAAAGGTAG+TGG 0.456680 5_2:-25570870 Msa0765090:CDS
TTTCTCTATACCGTCGTACT+TGG 0.474961 5_2:+25570742 None:intergenic
TAAATGGAAGGCTCACTTTG+AGG 0.488391 5_2:-25571738 Msa0765090:CDS
TCATTGATTTCACGGCTACT+TGG 0.488401 5_2:-25571144 Msa0765090:CDS
CTTTGTTAATATATTGCAAG+TGG 0.492939 5_2:+25570785 None:intergenic
CCATTTAGCAGTTGAGTGGA+AGG 0.494217 5_2:+25571754 None:intergenic
TTCTTCAATAGTATAAATGT+TGG 0.495545 5_2:+25570896 None:intergenic
ACACCAACTTAATTAGTACT+TGG 0.511925 5_2:+25570707 None:intergenic
GATTTCACGGCTACTTGGTG+TGG 0.515795 5_2:-25571139 Msa0765090:CDS
GTTGTGTAGGACCTTATATA+GGG 0.529533 5_2:+25571917 None:intergenic
ACATTTATACTATTGAAGAA+AGG 0.532669 5_2:-25570893 Msa0765090:CDS
GTGTGGACCTTGCAAATACA+TGG 0.544731 5_2:-25571122 Msa0765090:CDS
ATACCAAGTACTAATTAAGT+TGG 0.555978 5_2:-25570710 Msa0765090:three_prime_UTR
AAAGTAGTTGAAAAGGTAGT+GGG 0.557252 5_2:-25570869 Msa0765090:CDS
TTCCAAAGTAACAAACAAGC+TGG 0.568571 5_2:-25571714 Msa0765090:intron
AGAAGCTGATTGAGAAACGT+AGG 0.580578 5_2:-25570826 Msa0765090:CDS
CCTTCCATTTAGCAGTTGAG+TGG 0.582577 5_2:+25571750 None:intergenic
GTTATGTGTAAATGTTGTGT+AGG 0.583191 5_2:+25571904 None:intergenic
ATTGGGTCCATGTATTTGCA+AGG 0.586486 5_2:+25571115 None:intergenic
CCACTCAACTGCTAAATGGA+AGG 0.588835 5_2:-25571750 Msa0765090:CDS
GAAGCTGATTGAGAAACGTA+GGG 0.611562 5_2:-25570825 Msa0765090:CDS
GCTCCTATAGTACACATGCA+TGG 0.621114 5_2:+25570575 None:intergenic
TAGCAGTTGAGTGGAAGGTG+AGG 0.659322 5_2:+25571759 None:intergenic
TGAGTTCATTAAGATTGACG+TGG 0.684888 5_2:-25571059 Msa0765090:CDS
TGGAAGGTGAGGATGAGCGA+TGG 0.701264 5_2:+25571770 None:intergenic
TCAAGAGATACCAAGTACGA+CGG 0.717440 5_2:-25570752 Msa0765090:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTAATTATTTTTAAAGA+TGG - chr5_2:25571256-25571275 Msa0765090:intron 10.0%
!!! CATTGATAAATTATTATTTT+AGG + chr5_2:25571873-25571892 None:intergenic 10.0%
!!! TTCTTAATTATTTTTAAAGA+TGG - chr5_2:25571256-25571275 Msa0765090:intron 10.0%
!!! CATTGATAAATTATTATTTT+AGG + chr5_2:25571873-25571892 None:intergenic 10.0%
!!! TGTTAAATAATTTTTCTTAG+AGG - chr5_2:25570858-25570877 Msa0765090:CDS 15.0%
!!! GTTAAATAATTTTTCTTAGA+GGG - chr5_2:25570859-25570878 Msa0765090:CDS 15.0%
!!! GTTTTCATTTTTTTTTTTGT+TGG + chr5_2:25570941-25570960 None:intergenic 15.0%
!! TAATCAAATATATGTCATAT+AGG + chr5_2:25571119-25571138 None:intergenic 15.0%
!!! GCTAAAATTTTCTTATTATT+TGG - chr5_2:25571196-25571215 Msa0765090:intron 15.0%
!! TATAGATATAAGATATCAAA+AGG + chr5_2:25571433-25571452 None:intergenic 15.0%
!!! TGTTAAATAATTTTTCTTAG+AGG - chr5_2:25570858-25570877 Msa0765090:CDS 15.0%
!!! GTTAAATAATTTTTCTTAGA+GGG - chr5_2:25570859-25570878 Msa0765090:CDS 15.0%
!!! GTTTTCATTTTTTTTTTTGT+TGG + chr5_2:25570941-25570960 None:intergenic 15.0%
!! TAATCAAATATATGTCATAT+AGG + chr5_2:25571119-25571138 None:intergenic 15.0%
!!! GCTAAAATTTTCTTATTATT+TGG - chr5_2:25571196-25571215 Msa0765090:intron 15.0%
!! TATAGATATAAGATATCAAA+AGG + chr5_2:25571433-25571452 None:intergenic 15.0%
!!! TTTTTAGATACTTTGAAGTT+TGG + chr5_2:25570898-25570917 None:intergenic 20.0%
!!! TTGTTGGTAAATTAATCATA+TGG + chr5_2:25570925-25570944 None:intergenic 20.0%
!!! TAATAAGAAAATTTTAGCCA+AGG + chr5_2:25571194-25571213 None:intergenic 20.0%
!!! TTAAAATCGTTTGTTTTATG+AGG - chr5_2:25571220-25571239 Msa0765090:intron 20.0%
!! GATATCAAAAGGTAATTAAA+AGG + chr5_2:25571422-25571441 None:intergenic 20.0%
!! TTCTTCAATAGTATAAATGT+TGG + chr5_2:25571530-25571549 None:intergenic 20.0%
!! ACATTTATACTATTGAAGAA+AGG - chr5_2:25571530-25571549 Msa0765090:intron 20.0%
!!! TTTTTAGATACTTTGAAGTT+TGG + chr5_2:25570898-25570917 None:intergenic 20.0%
!!! TTGTTGGTAAATTAATCATA+TGG + chr5_2:25570925-25570944 None:intergenic 20.0%
!!! TAATAAGAAAATTTTAGCCA+AGG + chr5_2:25571194-25571213 None:intergenic 20.0%
!!! TTAAAATCGTTTGTTTTATG+AGG - chr5_2:25571220-25571239 Msa0765090:intron 20.0%
!! GATATCAAAAGGTAATTAAA+AGG + chr5_2:25571422-25571441 None:intergenic 20.0%
!! TTCTTCAATAGTATAAATGT+TGG + chr5_2:25571530-25571549 None:intergenic 20.0%
!! ACATTTATACTATTGAAGAA+AGG - chr5_2:25571530-25571549 Msa0765090:intron 20.0%
! TTGCTTAAGAATCAACATTA+TGG - chr5_2:25570583-25570602 Msa0765090:three_prime_UTR 25.0%
! TGCTTAAGAATCAACATTAT+GGG - chr5_2:25570584-25570603 Msa0765090:three_prime_UTR 25.0%
!! AAATTTTGTGACGAGAAAAA+CGG + chr5_2:25570969-25570988 None:intergenic 25.0%
!!! TTTTTCATCGGTTAAAATCT+TGG + chr5_2:25571006-25571025 None:intergenic 25.0%
! AAAACCAAGGTTTCAAAAAA+CGG - chr5_2:25571023-25571042 Msa0765090:intron 25.0%
! ATGACAACAATAACAATCAT+GGG + chr5_2:25571049-25571068 None:intergenic 25.0%
!! CAGCTAATTCTTTGATAATT+GGG + chr5_2:25571328-25571347 None:intergenic 25.0%
! CTTTGTTAATATATTGCAAG+TGG + chr5_2:25571641-25571660 None:intergenic 25.0%
! ACTTAATTAGTACTTGGTAT+TGG + chr5_2:25571713-25571732 None:intergenic 25.0%
! ATACCAAGTACTAATTAAGT+TGG - chr5_2:25571713-25571732 Msa0765090:intron 25.0%
! TTGCTTAAGAATCAACATTA+TGG - chr5_2:25570583-25570602 Msa0765090:three_prime_UTR 25.0%
! TGCTTAAGAATCAACATTAT+GGG - chr5_2:25570584-25570603 Msa0765090:three_prime_UTR 25.0%
!! AAATTTTGTGACGAGAAAAA+CGG + chr5_2:25570969-25570988 None:intergenic 25.0%
!!! TTTTTCATCGGTTAAAATCT+TGG + chr5_2:25571006-25571025 None:intergenic 25.0%
! AAAACCAAGGTTTCAAAAAA+CGG - chr5_2:25571023-25571042 Msa0765090:intron 25.0%
! ATGACAACAATAACAATCAT+GGG + chr5_2:25571049-25571068 None:intergenic 25.0%
!! CAGCTAATTCTTTGATAATT+GGG + chr5_2:25571328-25571347 None:intergenic 25.0%
! CTTTGTTAATATATTGCAAG+TGG + chr5_2:25571641-25571660 None:intergenic 25.0%
! ACTTAATTAGTACTTGGTAT+TGG + chr5_2:25571713-25571732 None:intergenic 25.0%
! ATACCAAGTACTAATTAAGT+TGG - chr5_2:25571713-25571732 Msa0765090:intron 25.0%
GTTATGTGTAAATGTTGTGT+AGG + chr5_2:25570522-25570541 None:intergenic 30.0%
AGACTCTAAATTGGAAAAGT+TGG + chr5_2:25570612-25570631 None:intergenic 30.0%
TAACATTGAATGATTCGATG+AGG + chr5_2:25570801-25570820 None:intergenic 30.0%
TTTAACCGATGAAAAAACCA+AGG - chr5_2:25571010-25571029 Msa0765090:intron 30.0%
!!! TGAAACCTTGGTTTTTTCAT+CGG + chr5_2:25571018-25571037 None:intergenic 30.0%
CATGACAACAATAACAATCA+TGG + chr5_2:25571050-25571069 None:intergenic 30.0%
! CTTAACATTTTCTTTGCACT+CGG - chr5_2:25571081-25571100 Msa0765090:CDS 30.0%
!!! TTAGCCAAGGTTTTAAATCA+CGG + chr5_2:25571181-25571200 None:intergenic 30.0%
!!! AAATCGTTTGTTTTATGAGG+TGG - chr5_2:25571223-25571242 Msa0765090:intron 30.0%
!! AAAGATGGTCATTGATTTCA+CGG - chr5_2:25571271-25571290 Msa0765090:intron 30.0%
! GCAGCTAATTCTTTGATAAT+TGG + chr5_2:25571329-25571348 None:intergenic 30.0%
GATAATGCAAAATGTACCAA+TGG - chr5_2:25571465-25571484 Msa0765090:intron 30.0%
GAAAGGAAAAGTAGTTGAAA+AGG - chr5_2:25571547-25571566 Msa0765090:intron 30.0%
AAAAGTAGTTGAAAAGGTAG+TGG - chr5_2:25571553-25571572 Msa0765090:intron 30.0%
AAAGTAGTTGAAAAGGTAGT+GGG - chr5_2:25571554-25571573 Msa0765090:intron 30.0%
ACACCAACTTAATTAGTACT+TGG + chr5_2:25571719-25571738 None:intergenic 30.0%
GTTATGTGTAAATGTTGTGT+AGG + chr5_2:25570522-25570541 None:intergenic 30.0%
AGACTCTAAATTGGAAAAGT+TGG + chr5_2:25570612-25570631 None:intergenic 30.0%
TAACATTGAATGATTCGATG+AGG + chr5_2:25570801-25570820 None:intergenic 30.0%
TTTAACCGATGAAAAAACCA+AGG - chr5_2:25571010-25571029 Msa0765090:intron 30.0%
!!! TGAAACCTTGGTTTTTTCAT+CGG + chr5_2:25571018-25571037 None:intergenic 30.0%
CATGACAACAATAACAATCA+TGG + chr5_2:25571050-25571069 None:intergenic 30.0%
! CTTAACATTTTCTTTGCACT+CGG - chr5_2:25571081-25571100 Msa0765090:CDS 30.0%
!!! TTAGCCAAGGTTTTAAATCA+CGG + chr5_2:25571181-25571200 None:intergenic 30.0%
!!! AAATCGTTTGTTTTATGAGG+TGG - chr5_2:25571223-25571242 Msa0765090:intron 30.0%
!! AAAGATGGTCATTGATTTCA+CGG - chr5_2:25571271-25571290 Msa0765090:intron 30.0%
! GCAGCTAATTCTTTGATAAT+TGG + chr5_2:25571329-25571348 None:intergenic 30.0%
GATAATGCAAAATGTACCAA+TGG - chr5_2:25571465-25571484 Msa0765090:intron 30.0%
GAAAGGAAAAGTAGTTGAAA+AGG - chr5_2:25571547-25571566 Msa0765090:intron 30.0%
AAAAGTAGTTGAAAAGGTAG+TGG - chr5_2:25571553-25571572 Msa0765090:intron 30.0%
AAAGTAGTTGAAAAGGTAGT+GGG - chr5_2:25571554-25571573 Msa0765090:intron 30.0%
ACACCAACTTAATTAGTACT+TGG + chr5_2:25571719-25571738 None:intergenic 30.0%
GTTGTGTAGGACCTTATATA+GGG + chr5_2:25570509-25570528 None:intergenic 35.0%
TGTTGTGTAGGACCTTATAT+AGG + chr5_2:25570510-25570529 None:intergenic 35.0%
CTCAACAACAGACTCTAAAT+TGG + chr5_2:25570621-25570640 None:intergenic 35.0%
TTCCAAAGTAACAAACAAGC+TGG - chr5_2:25570709-25570728 Msa0765090:three_prime_UTR 35.0%
! TACCAGCTTGTTTGTTACTT+TGG + chr5_2:25570714-25570733 None:intergenic 35.0%
TGAGTTCATTAAGATTGACG+TGG - chr5_2:25571364-25571383 Msa0765090:intron 35.0%
CAAAATGTACCAATGGAAAC+AGG - chr5_2:25571472-25571491 Msa0765090:intron 35.0%
! CTTCTGTAATTGCTCCTTTT+TGG + chr5_2:25571582-25571601 None:intergenic 35.0%
!!! ATTTTTTGAAGGAGCATGCA+TGG + chr5_2:25571810-25571829 None:intergenic 35.0%
TATCCATGCATGTGTACTAT+AGG - chr5_2:25571845-25571864 Msa0765090:five_prime_UTR 35.0%
GTTGTGTAGGACCTTATATA+GGG + chr5_2:25570509-25570528 None:intergenic 35.0%
TGTTGTGTAGGACCTTATAT+AGG + chr5_2:25570510-25570529 None:intergenic 35.0%
CTCAACAACAGACTCTAAAT+TGG + chr5_2:25570621-25570640 None:intergenic 35.0%
TTCCAAAGTAACAAACAAGC+TGG - chr5_2:25570709-25570728 Msa0765090:three_prime_UTR 35.0%
! TACCAGCTTGTTTGTTACTT+TGG + chr5_2:25570714-25570733 None:intergenic 35.0%
TGAGTTCATTAAGATTGACG+TGG - chr5_2:25571364-25571383 Msa0765090:intron 35.0%
CAAAATGTACCAATGGAAAC+AGG - chr5_2:25571472-25571491 Msa0765090:intron 35.0%
! CTTCTGTAATTGCTCCTTTT+TGG + chr5_2:25571582-25571601 None:intergenic 35.0%
!!! ATTTTTTGAAGGAGCATGCA+TGG + chr5_2:25571810-25571829 None:intergenic 35.0%
TATCCATGCATGTGTACTAT+AGG - chr5_2:25571845-25571864 Msa0765090:five_prime_UTR 35.0%
TAAATGGAAGGCTCACTTTG+AGG - chr5_2:25570685-25570704 Msa0765090:three_prime_UTR 40.0%
!!! AAGGTTTTAAATCACGGTCG+TGG + chr5_2:25571175-25571194 None:intergenic 40.0%
! ACGACCGTGATTTAAAACCT+TGG - chr5_2:25571174-25571193 Msa0765090:intron 40.0%
!! TCATTGATTTCACGGCTACT+TGG - chr5_2:25571279-25571298 Msa0765090:intron 40.0%
ATTGGGTCCATGTATTTGCA+AGG + chr5_2:25571311-25571330 None:intergenic 40.0%
GATTGACGTGGATGAGTTAA+TGG - chr5_2:25571376-25571395 Msa0765090:intron 40.0%
AATGTACCAATGGAAACAGG+AGG - chr5_2:25571475-25571494 Msa0765090:intron 40.0%
AGAAGCTGATTGAGAAACGT+AGG - chr5_2:25571597-25571616 Msa0765090:intron 40.0%
GAAGCTGATTGAGAAACGTA+GGG - chr5_2:25571598-25571617 Msa0765090:intron 40.0%
TCAAGAGATACCAAGTACGA+CGG - chr5_2:25571671-25571690 Msa0765090:intron 40.0%
TTTCTCTATACCGTCGTACT+TGG + chr5_2:25571684-25571703 None:intergenic 40.0%
TAAATGGAAGGCTCACTTTG+AGG - chr5_2:25570685-25570704 Msa0765090:three_prime_UTR 40.0%
!!! AAGGTTTTAAATCACGGTCG+TGG + chr5_2:25571175-25571194 None:intergenic 40.0%
! ACGACCGTGATTTAAAACCT+TGG - chr5_2:25571174-25571193 Msa0765090:intron 40.0%
!! TCATTGATTTCACGGCTACT+TGG - chr5_2:25571279-25571298 Msa0765090:intron 40.0%
ATTGGGTCCATGTATTTGCA+AGG + chr5_2:25571311-25571330 None:intergenic 40.0%
GATTGACGTGGATGAGTTAA+TGG - chr5_2:25571376-25571395 Msa0765090:intron 40.0%
AATGTACCAATGGAAACAGG+AGG - chr5_2:25571475-25571494 Msa0765090:intron 40.0%
AGAAGCTGATTGAGAAACGT+AGG - chr5_2:25571597-25571616 Msa0765090:intron 40.0%
GAAGCTGATTGAGAAACGTA+GGG - chr5_2:25571598-25571617 Msa0765090:intron 40.0%
TCAAGAGATACCAAGTACGA+CGG - chr5_2:25571671-25571690 Msa0765090:intron 40.0%
TTTCTCTATACCGTCGTACT+TGG + chr5_2:25571684-25571703 None:intergenic 40.0%
CCATTTAGCAGTTGAGTGGA+AGG + chr5_2:25570672-25570691 None:intergenic 45.0%
CCTTCCACTCAACTGCTAAA+TGG - chr5_2:25570669-25570688 Msa0765090:three_prime_UTR 45.0%
CCTTCCATTTAGCAGTTGAG+TGG + chr5_2:25570676-25570695 None:intergenic 45.0%
CCACTCAACTGCTAAATGGA+AGG - chr5_2:25570673-25570692 Msa0765090:three_prime_UTR 45.0%
ACAACGATGCTCACGTAGTT+TGG - chr5_2:25570762-25570781 Msa0765090:three_prime_UTR 45.0%
!!! TGGGCCGTTTTTTGAAACCT+TGG + chr5_2:25571030-25571049 None:intergenic 45.0%
GTGTGGACCTTGCAAATACA+TGG - chr5_2:25571301-25571320 Msa0765090:intron 45.0%
!! AAAGGTAGTGGGAGCCAAAA+AGG - chr5_2:25571565-25571584 Msa0765090:intron 45.0%
GCTCCTATAGTACACATGCA+TGG + chr5_2:25571851-25571870 None:intergenic 45.0%
CCATTTAGCAGTTGAGTGGA+AGG + chr5_2:25570672-25570691 None:intergenic 45.0%
CCTTCCACTCAACTGCTAAA+TGG - chr5_2:25570669-25570688 Msa0765090:three_prime_UTR 45.0%
CCTTCCATTTAGCAGTTGAG+TGG + chr5_2:25570676-25570695 None:intergenic 45.0%
CCACTCAACTGCTAAATGGA+AGG - chr5_2:25570673-25570692 Msa0765090:three_prime_UTR 45.0%
ACAACGATGCTCACGTAGTT+TGG - chr5_2:25570762-25570781 Msa0765090:three_prime_UTR 45.0%
!!! TGGGCCGTTTTTTGAAACCT+TGG + chr5_2:25571030-25571049 None:intergenic 45.0%
GTGTGGACCTTGCAAATACA+TGG - chr5_2:25571301-25571320 Msa0765090:intron 45.0%
!! AAAGGTAGTGGGAGCCAAAA+AGG - chr5_2:25571565-25571584 Msa0765090:intron 45.0%
GCTCCTATAGTACACATGCA+TGG + chr5_2:25571851-25571870 None:intergenic 45.0%
!!! TATTTATTATTATTTTTTGA+AGG + chr5_2:25571821-25571840 None:intergenic 5.0%
!!! TATTTATTATTATTTTTTGA+AGG + chr5_2:25571821-25571840 None:intergenic 5.0%
TAGCAGTTGAGTGGAAGGTG+AGG + chr5_2:25570667-25570686 None:intergenic 50.0%
! GATTTCACGGCTACTTGGTG+TGG - chr5_2:25571284-25571303 Msa0765090:intron 50.0%
GTACCAATGGAAACAGGAGG+TGG - chr5_2:25571478-25571497 Msa0765090:intron 50.0%
TTGCCACCTCCTGTTTCCAT+TGG + chr5_2:25571484-25571503 None:intergenic 50.0%
GGAGGTGGCAAATGCATTTC+AGG - chr5_2:25571493-25571512 Msa0765090:intron 50.0%
TAGCAGTTGAGTGGAAGGTG+AGG + chr5_2:25570667-25570686 None:intergenic 50.0%
! GATTTCACGGCTACTTGGTG+TGG - chr5_2:25571284-25571303 Msa0765090:intron 50.0%
GTACCAATGGAAACAGGAGG+TGG - chr5_2:25571478-25571497 Msa0765090:intron 50.0%
TTGCCACCTCCTGTTTCCAT+TGG + chr5_2:25571484-25571503 None:intergenic 50.0%
GGAGGTGGCAAATGCATTTC+AGG - chr5_2:25571493-25571512 Msa0765090:intron 50.0%
TGGAAGGTGAGGATGAGCGA+TGG + chr5_2:25570656-25570675 None:intergenic 55.0%
TGGAAGGTGAGGATGAGCGA+TGG + chr5_2:25570656-25570675 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 25570505 25571940 25570505 ID=Msa0765090;Name=Msa0765090
chr5_2 mRNA 25570505 25571940 25570505 ID=Msa0765090-mRNA-1;Parent=Msa0765090;Name=Msa0765090-mRNA-1;_AED=0.05;_eAED=0.05;_QI=97|1|1|1|1|1|3|315|127
chr5_2 exon 25570505 25570951 25570505 ID=Msa0765090-mRNA-1:exon:10278;Parent=Msa0765090-mRNA-1
chr5_2 exon 25571048 25571170 25571048 ID=Msa0765090-mRNA-1:exon:10277;Parent=Msa0765090-mRNA-1
chr5_2 exon 25571715 25571940 25571715 ID=Msa0765090-mRNA-1:exon:10276;Parent=Msa0765090-mRNA-1
chr5_2 five_prime_UTR 25571844 25571940 25571844 ID=Msa0765090-mRNA-1:five_prime_utr;Parent=Msa0765090-mRNA-1
chr5_2 CDS 25571715 25571843 25571715 ID=Msa0765090-mRNA-1:cds;Parent=Msa0765090-mRNA-1
chr5_2 CDS 25571048 25571170 25571048 ID=Msa0765090-mRNA-1:cds;Parent=Msa0765090-mRNA-1
chr5_2 CDS 25570820 25570951 25570820 ID=Msa0765090-mRNA-1:cds;Parent=Msa0765090-mRNA-1
chr5_2 three_prime_UTR 25570505 25570819 25570505 ID=Msa0765090-mRNA-1:three_prime_utr;Parent=Msa0765090-mRNA-1
Gene Sequence

>Msa0765090

ATGGGAGCCAACTTTTCCAATTTAGAGTCTGTTGTTGAGAAATCATCACAGCCATCGCTCATCCTCACCTTCCACTCAACTGCTAAATGGAAGGCTCACTTTGAGGCTTCCAAAGTAACAAACAAGCTGATGGTCATTGATTTCACGGCTACTTGGTGTGGACCTTGCAAATACATGGACCCAATTATCAAAGAATTAGCTGCAAAATACAAAGATGTTGAGTTCATTAAGATTGACGTGGATGAGTTAATGGAGGTGGCAAATGCATTTCAGGTGCAAGCAATGCCAACATTTATACTATTGAAGAAAGGAAAAGTAGTTGAAAAGGTAGTGGGAGCCAAAAAGGAGCAATTACAGAAGCTGATTGAGAAACGTAGGGTTTGA
Protein sequence

>Msa0765090

MGANFSNLESVVEKSSQPSLILTFHSTAKWKAHFEASKVTNKLMVIDFTATWCGPCKYMDPIIKELAAKYKDVEFIKIDVDELMEVANAFQVQAMPTFILLKKGKVVEKVVGAKKEQLQKLIEKRRV*