Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791930 | KEH36435.1 | 98.571 | 70 | 1 | 0 | 1 | 70 | 37 | 106 | 1.87e-41 | 140 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791930 | sp|Q9M0N8|MZT1B_ARATH | 73.913 | 69 | 17 | 1 | 1 | 69 | 1 | 68 | 1.55e-30 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791930 | A0A072VE60 | 98.571 | 70 | 1 | 0 | 1 | 70 | 37 | 106 | 8.95e-42 | 140 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0791930 | -0.811702 | 6.132963e-51 | -8.615850e-47 |
| Msa0024210 | Msa0791930 | 0.811410 | 7.099005e-51 | -8.615850e-47 |
| Msa0120010 | Msa0791930 | 0.800100 | 1.688354e-48 | -8.615850e-47 |
| Msa0120020 | Msa0791930 | 0.810719 | 1.002391e-50 | -8.615850e-47 |
| Msa0120040 | Msa0791930 | 0.800724 | 1.260322e-48 | -8.615850e-47 |
| Msa0791930 | Msa0880140 | -0.805652 | 1.203755e-49 | -8.615850e-47 |
| Msa0791930 | Msa0947660 | -0.811930 | 5.473224e-51 | -8.615850e-47 |
| Msa0791930 | Msa1193290 | -0.805123 | 1.553572e-49 | -8.615850e-47 |
| Msa0791930 | Msa1360060 | -0.801018 | 1.097228e-48 | -8.615850e-47 |
| Msa0791930 | Msa1372760 | -0.810974 | 8.824779e-51 | -8.615850e-47 |
| Msa0791930 | Msa1374550 | -0.803288 | 3.743822e-49 | -8.615850e-47 |
| Msa0791930 | Msa1386450 | 0.966791 | 2.146822e-126 | -8.615850e-47 |
| Msa0140780 | Msa0791930 | -0.803048 | 4.197384e-49 | -8.615850e-47 |
| Msa0157290 | Msa0791930 | -0.811689 | 6.173717e-51 | -8.615850e-47 |
| Msa0243100 | Msa0791930 | -0.811658 | 6.270528e-51 | -8.615850e-47 |
| Msa0287650 | Msa0791930 | -0.805484 | 1.305621e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791930 | MtrunA17_Chr2g0278751 | 98.571 | 70 | 1 | 0 | 1 | 70 | 1 | 70 | 4.95e-45 | 138 |
| Msa0791930 | MtrunA17_Chr4g0069421 | 97.143 | 70 | 2 | 0 | 1 | 70 | 1 | 70 | 1.38e-43 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791930 | AT4G09550.1 | 73.913 | 69 | 17 | 1 | 1 | 69 | 1 | 68 | 1.57e-31 | 104 |
| Msa0791930 | AT4G09550.2 | 73.913 | 69 | 17 | 1 | 1 | 69 | 1 | 68 | 1.57e-31 | 104 |
| Msa0791930 | AT1G73790.1 | 72.414 | 58 | 16 | 0 | 1 | 58 | 1 | 58 | 3.78e-27 | 93.6 |
Find 23 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTGCACTTCTTGCAGCTTC+AGG | 0.145529 | 5_3:+3017970 | None:intergenic |
| ACAAAATACATTCATGATTT+AGG | 0.172021 | 5_3:+3017769 | None:intergenic |
| CTCATTGCCCTCTGTGATCT+TGG | 0.190398 | 5_3:-3017874 | Msa0791930:CDS |
| TCATTGCCCTCTGTGATCTT+GGG | 0.281322 | 5_3:-3017873 | Msa0791930:CDS |
| ACAATAGCAGCAAGTGCTTC+AGG | 0.324395 | 5_3:+3017844 | None:intergenic |
| TAAACCCGTGTCGAGTATAT+TGG | 0.366888 | 5_3:+3017918 | None:intergenic |
| AAGAAGTGCACGAGAATCTT+TGG | 0.388040 | 5_3:-3017959 | Msa0791930:CDS |
| GTGCACTTCTTGCAGCTTCA+GGG | 0.392834 | 5_3:+3017971 | None:intergenic |
| CATTGCCCTCTGTGATCTTG+GGG | 0.494922 | 5_3:-3017872 | Msa0791930:CDS |
| TAGGATGCAATGATGACATT+GGG | 0.500215 | 5_3:+3017797 | None:intergenic |
| TATTGTCAAGGAACTTAGAA+AGG | 0.527792 | 5_3:-3017827 | Msa0791930:CDS |
| ATTCATGATTTAGGAGGCGT+AGG | 0.534552 | 5_3:+3017778 | None:intergenic |
| AAATACATTCATGATTTAGG+AGG | 0.583464 | 5_3:+3017772 | None:intergenic |
| TATTGGACATGTGAAATGCC+AGG | 0.592806 | 5_3:+3017935 | None:intergenic |
| GTAGGATGCAATGATGACAT+TGG | 0.595456 | 5_3:+3017796 | None:intergenic |
| TGCACGAGAATCTTTGGACC+TGG | 0.595713 | 5_3:-3017953 | Msa0791930:CDS |
| CATGTCCAATATACTCGACA+CGG | 0.596616 | 5_3:-3017923 | Msa0791930:CDS |
| AGCACTTGCTGCTATTGTCA+AGG | 0.625051 | 5_3:-3017839 | Msa0791930:CDS |
| TCAAGGAACTTAGAAAGGAG+AGG | 0.641589 | 5_3:-3017822 | Msa0791930:CDS |
| ATTGACCCCAAGATCACAGA+GGG | 0.646842 | 5_3:+3017867 | None:intergenic |
| TGAGAATAGAAAGTGCATGA+CGG | 0.680398 | 5_3:+3017893 | None:intergenic |
| ATGTCCAATATACTCGACAC+GGG | 0.686297 | 5_3:-3017922 | Msa0791930:CDS |
| GATTGACCCCAAGATCACAG+AGG | 0.775929 | 5_3:+3017866 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTGTCAAGGAACTTAGAA+AGG | - | chr5_3:3017929-3017948 | Msa0791930:CDS | 30.0% | |
| ! | TGAGAATAGAAAGTGCATGA+CGG | + | chr5_3:3017866-3017885 | None:intergenic | 35.0% |
| TAGGATGCAATGATGACATT+GGG | + | chr5_3:3017962-3017981 | None:intergenic | 35.0% | |
| ! | AAGAAGTGCACGAGAATCTT+TGG | - | chr5_3:3017797-3017816 | Msa0791930:CDS | 40.0% |
| TATTGGACATGTGAAATGCC+AGG | + | chr5_3:3017824-3017843 | None:intergenic | 40.0% | |
| CATGTCCAATATACTCGACA+CGG | - | chr5_3:3017833-3017852 | Msa0791930:CDS | 40.0% | |
| ATGTCCAATATACTCGACAC+GGG | - | chr5_3:3017834-3017853 | Msa0791930:CDS | 40.0% | |
| TAAACCCGTGTCGAGTATAT+TGG | + | chr5_3:3017841-3017860 | None:intergenic | 40.0% | |
| TCAAGGAACTTAGAAAGGAG+AGG | - | chr5_3:3017934-3017953 | Msa0791930:CDS | 40.0% | |
| GTAGGATGCAATGATGACAT+TGG | + | chr5_3:3017963-3017982 | None:intergenic | 40.0% | |
| TCATTGCCCTCTGTGATCTT+GGG | - | chr5_3:3017883-3017902 | Msa0791930:CDS | 45.0% | |
| ATTGACCCCAAGATCACAGA+GGG | + | chr5_3:3017892-3017911 | None:intergenic | 45.0% | |
| ACAATAGCAGCAAGTGCTTC+AGG | + | chr5_3:3017915-3017934 | None:intergenic | 45.0% | |
| ! | AGCACTTGCTGCTATTGTCA+AGG | - | chr5_3:3017917-3017936 | Msa0791930:CDS | 45.0% |
| GTGCACTTCTTGCAGCTTCA+GGG | + | chr5_3:3017788-3017807 | None:intergenic | 50.0% | |
| TGCACGAGAATCTTTGGACC+TGG | - | chr5_3:3017803-3017822 | Msa0791930:CDS | 50.0% | |
| CTCATTGCCCTCTGTGATCT+TGG | - | chr5_3:3017882-3017901 | Msa0791930:CDS | 50.0% | |
| CATTGCCCTCTGTGATCTTG+GGG | - | chr5_3:3017884-3017903 | Msa0791930:CDS | 50.0% | |
| GATTGACCCCAAGATCACAG+AGG | + | chr5_3:3017893-3017912 | None:intergenic | 50.0% | |
| CGTGCACTTCTTGCAGCTTC+AGG | + | chr5_3:3017789-3017808 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 3017780 | 3017998 | 3017780 | ID=Msa0791930;Name=Msa0791930 |
| chr5_3 | mRNA | 3017780 | 3017998 | 3017780 | ID=Msa0791930-mRNA-1;Parent=Msa0791930;Name=Msa0791930-mRNA-1;_AED=0.40;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|72 |
| chr5_3 | exon | 3017780 | 3017998 | 3017780 | ID=Msa0791930-mRNA-1:exon:1328;Parent=Msa0791930-mRNA-1 |
| chr5_3 | CDS | 3017780 | 3017998 | 3017780 | ID=Msa0791930-mRNA-1:cds;Parent=Msa0791930-mRNA-1 |
| Gene Sequence |
| Protein sequence |
