Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386450 | KEH36435.1 | 98.571 | 70 | 1 | 0 | 1 | 70 | 37 | 106 | 1.87e-41 | 140 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386450 | sp|Q9M0N8|MZT1B_ARATH | 73.913 | 69 | 17 | 1 | 1 | 69 | 1 | 68 | 1.55e-30 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386450 | A0A072VE60 | 98.571 | 70 | 1 | 0 | 1 | 70 | 37 | 106 | 8.95e-42 | 140 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa1386450 | -0.837655 | 4.573623e-57 | -8.615850e-47 |
| Msa0024210 | Msa1386450 | 0.826339 | 2.864908e-54 | -8.615850e-47 |
| Msa0028640 | Msa1386450 | -0.807199 | 5.680890e-50 | -8.615850e-47 |
| Msa0110970 | Msa1386450 | -0.817373 | 3.410504e-52 | -8.615850e-47 |
| Msa0120010 | Msa1386450 | 0.826606 | 2.474418e-54 | -8.615850e-47 |
| Msa0120020 | Msa1386450 | 0.830171 | 3.412598e-55 | -8.615850e-47 |
| Msa0120040 | Msa1386450 | 0.815520 | 8.865139e-52 | -8.615850e-47 |
| Msa1346370 | Msa1386450 | -0.800130 | 1.664901e-48 | -8.615850e-47 |
| Msa1360060 | Msa1386450 | -0.813667 | 2.279132e-51 | -8.615850e-47 |
| Msa1372760 | Msa1386450 | -0.829389 | 5.290827e-55 | -8.615850e-47 |
| Msa1374550 | Msa1386450 | -0.816859 | 4.449349e-52 | -8.615850e-47 |
| Msa1386450 | Msa1409870 | -0.820316 | 7.315504e-53 | -8.615850e-47 |
| Msa1386450 | Msa1411020 | -0.805411 | 1.352141e-49 | -8.615850e-47 |
| Msa1386450 | Msa1424290 | -0.807308 | 5.385482e-50 | -8.615850e-47 |
| Msa1386450 | Msa1434350 | -0.800103 | 1.686364e-48 | -8.615850e-47 |
| Msa1386450 | Msa1439320 | -0.810924 | 9.049271e-51 | -8.615850e-47 |
| Msa1386450 | Msa1461300 | -0.802841 | 4.632921e-49 | -8.615850e-47 |
| Msa1386450 | Msa1466540 | -0.815954 | 7.093429e-52 | -8.615850e-47 |
| Msa0616160 | Msa1386450 | -0.800102 | 1.687358e-48 | -8.615850e-47 |
| Msa0648880 | Msa1386450 | -0.801945 | 7.084950e-49 | -8.615850e-47 |
| Msa0657970 | Msa1386450 | -0.819811 | 9.548568e-53 | -8.615850e-47 |
| Msa0663380 | Msa1386450 | -0.804909 | 1.722333e-49 | -8.615850e-47 |
| Msa0663390 | Msa1386450 | -0.801829 | 7.484053e-49 | -8.615850e-47 |
| Msa0673460 | Msa1386450 | -0.811747 | 5.998767e-51 | -8.615850e-47 |
| Msa0688480 | Msa1386450 | -0.800153 | 1.647050e-48 | -8.615850e-47 |
| Msa0735170 | Msa1386450 | -0.800671 | 1.291919e-48 | -8.615850e-47 |
| Msa0743720 | Msa1386450 | -0.813930 | 1.994191e-51 | -8.615850e-47 |
| Msa0757500 | Msa1386450 | 0.805692 | 1.180594e-49 | -8.615850e-47 |
| Msa0791930 | Msa1386450 | 0.966791 | 2.146822e-126 | -8.615850e-47 |
| Msa0822870 | Msa1386450 | -0.817293 | 3.554464e-52 | -8.615850e-47 |
| Msa0832340 | Msa1386450 | -0.806914 | 6.526735e-50 | -8.615850e-47 |
| Msa0140780 | Msa1386450 | -0.815555 | 8.705193e-52 | -8.615850e-47 |
| Msa0157290 | Msa1386450 | -0.837487 | 5.050403e-57 | -8.615850e-47 |
| Msa0169860 | Msa1386450 | -0.804848 | 1.773831e-49 | -8.615850e-47 |
| Msa0200260 | Msa1386450 | -0.808985 | 2.366332e-50 | -8.615850e-47 |
| Msa0212990 | Msa1386450 | -0.806806 | 6.879841e-50 | -8.615850e-47 |
| Msa0215320 | Msa1386450 | 0.812438 | 4.239740e-51 | -8.615850e-47 |
| Msa1244250 | Msa1386450 | -0.802294 | 6.005510e-49 | -8.615850e-47 |
| Msa1253020 | Msa1386450 | -0.800473 | 1.417412e-48 | -8.615850e-47 |
| Msa1284680 | Msa1386450 | -0.805014 | 1.637981e-49 | -8.615850e-47 |
| Msa1308380 | Msa1386450 | -0.818508 | 1.889848e-52 | -8.615850e-47 |
| Msa1320410 | Msa1386450 | -0.802708 | 4.935886e-49 | -8.615850e-47 |
| Msa1327050 | Msa1386450 | -0.806715 | 7.190329e-50 | -8.615850e-47 |
| Msa1327680 | Msa1386450 | -0.808784 | 2.612520e-50 | -8.615850e-47 |
| Msa0393080 | Msa1386450 | -0.817840 | 2.676711e-52 | -8.615850e-47 |
| Msa0410650 | Msa1386450 | -0.813517 | 2.458917e-51 | -8.615850e-47 |
| Msa0417880 | Msa1386450 | -0.818866 | 1.567237e-52 | -8.615850e-47 |
| Msa0440750 | Msa1386450 | -0.801123 | 1.044536e-48 | -8.615850e-47 |
| Msa1045200 | Msa1386450 | -0.806585 | 7.660459e-50 | -8.615850e-47 |
| Msa1075220 | Msa1386450 | 0.815910 | 7.255622e-52 | -8.615850e-47 |
| Msa1082500 | Msa1386450 | -0.818839 | 1.589666e-52 | -8.615850e-47 |
| Msa1085200 | Msa1386450 | -0.811235 | 7.748068e-51 | -8.615850e-47 |
| Msa0243100 | Msa1386450 | -0.824005 | 1.020342e-53 | -8.615850e-47 |
| Msa0244860 | Msa1386450 | -0.805982 | 1.025985e-49 | -8.615850e-47 |
| Msa0283450 | Msa1386450 | -0.823292 | 1.498105e-53 | -8.615850e-47 |
| Msa0283520 | Msa1386450 | -0.811443 | 6.983825e-51 | -8.615850e-47 |
| Msa0287410 | Msa1386450 | -0.803642 | 3.162297e-49 | -8.615850e-47 |
| Msa0287650 | Msa1386450 | -0.824198 | 9.190726e-54 | -8.615850e-47 |
| Msa0291540 | Msa1386450 | -0.817035 | 4.062021e-52 | -8.615850e-47 |
| Msa0294560 | Msa1386450 | 0.802127 | 6.502087e-49 | -8.615850e-47 |
| Msa0306210 | Msa1386450 | 0.805041 | 1.616428e-49 | -8.615850e-47 |
| Msa0332000 | Msa1386450 | -0.820761 | 5.781295e-53 | -8.615850e-47 |
| Msa0340470 | Msa1386450 | -0.800074 | 1.709429e-48 | -8.615850e-47 |
| Msa0357340 | Msa1386450 | -0.821535 | 3.834790e-53 | -8.615850e-47 |
| Msa1116130 | Msa1386450 | 0.818283 | 2.125133e-52 | -8.615850e-47 |
| Msa1137570 | Msa1386450 | -0.806195 | 9.256694e-50 | -8.615850e-47 |
| Msa1162550 | Msa1386450 | 0.812478 | 4.154630e-51 | -8.615850e-47 |
| Msa1186950 | Msa1386450 | 0.808568 | 2.905981e-50 | -8.615850e-47 |
| Msa1193290 | Msa1386450 | -0.825927 | 3.589099e-54 | -8.615850e-47 |
| Msa1199830 | Msa1386450 | 0.802236 | 6.173170e-49 | -8.615850e-47 |
| Msa0484780 | Msa1386450 | -0.805818 | 1.110714e-49 | -8.615850e-47 |
| Msa0488560 | Msa1386450 | -0.812301 | 4.541252e-51 | -8.615850e-47 |
| Msa0507680 | Msa1386450 | -0.815172 | 1.059204e-51 | -8.615850e-47 |
| Msa0532350 | Msa1386450 | -0.801047 | 1.082501e-48 | -8.615850e-47 |
| Msa0844570 | Msa1386450 | -0.801801 | 7.587210e-49 | -8.615850e-47 |
| Msa0880140 | Msa1386450 | -0.831823 | 1.341333e-55 | -8.615850e-47 |
| Msa0925290 | Msa1386450 | -0.811264 | 7.636758e-51 | -8.615850e-47 |
| Msa0938220 | Msa1386450 | -0.802749 | 4.839952e-49 | -8.615850e-47 |
| Msa0947660 | Msa1386450 | -0.823161 | 1.607539e-53 | -8.615850e-47 |
| Msa0969520 | Msa1386450 | -0.816326 | 5.858894e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386450 | MtrunA17_Chr2g0278751 | 98.571 | 70 | 1 | 0 | 1 | 70 | 1 | 70 | 4.95e-45 | 138 |
| Msa1386450 | MtrunA17_Chr4g0069421 | 97.143 | 70 | 2 | 0 | 1 | 70 | 1 | 70 | 1.38e-43 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386450 | AT4G09550.1 | 73.913 | 69 | 17 | 1 | 1 | 69 | 1 | 68 | 1.57e-31 | 104 |
| Msa1386450 | AT4G09550.2 | 73.913 | 69 | 17 | 1 | 1 | 69 | 1 | 68 | 1.57e-31 | 104 |
| Msa1386450 | AT1G73790.1 | 72.414 | 58 | 16 | 0 | 1 | 58 | 1 | 58 | 3.78e-27 | 93.6 |
Find 23 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTGCACTTCTTGCAGCTTC+AGG | 0.145529 | tig0020816:+74415 | None:intergenic |
| ACAAAATACATTCATGATTT+AGG | 0.172021 | tig0020816:+74214 | None:intergenic |
| CTCATTGCCCTCTGTGATCT+TGG | 0.190398 | tig0020816:-74319 | Msa1386450:CDS |
| TCATTGCCCTCTGTGATCTT+GGG | 0.281322 | tig0020816:-74318 | Msa1386450:CDS |
| ACAATAGCAGCAAGTGCTTC+AGG | 0.324395 | tig0020816:+74289 | None:intergenic |
| TAAACCCGTGTCGAGTATAT+TGG | 0.366888 | tig0020816:+74363 | None:intergenic |
| AAGAAGTGCACGAGAATCTT+TGG | 0.388040 | tig0020816:-74404 | Msa1386450:CDS |
| GTGCACTTCTTGCAGCTTCA+GGG | 0.392834 | tig0020816:+74416 | None:intergenic |
| CATTGCCCTCTGTGATCTTG+GGG | 0.494922 | tig0020816:-74317 | Msa1386450:CDS |
| TAGGATGCAATGATGACATT+GGG | 0.500215 | tig0020816:+74242 | None:intergenic |
| TATTGTCAAGGAACTTAGAA+AGG | 0.527792 | tig0020816:-74272 | Msa1386450:CDS |
| ATTCATGATTTAGGAGGCGT+AGG | 0.534552 | tig0020816:+74223 | None:intergenic |
| AAATACATTCATGATTTAGG+AGG | 0.583464 | tig0020816:+74217 | None:intergenic |
| TATTGGACATGTGAAATGCC+AGG | 0.592806 | tig0020816:+74380 | None:intergenic |
| GTAGGATGCAATGATGACAT+TGG | 0.595456 | tig0020816:+74241 | None:intergenic |
| TGCACGAGAATCTTTGGACC+TGG | 0.595713 | tig0020816:-74398 | Msa1386450:CDS |
| CATGTCCAATATACTCGACA+CGG | 0.596616 | tig0020816:-74368 | Msa1386450:CDS |
| AGCACTTGCTGCTATTGTCA+AGG | 0.625051 | tig0020816:-74284 | Msa1386450:CDS |
| TCAAGGAACTTAGAAAGGAG+AGG | 0.641589 | tig0020816:-74267 | Msa1386450:CDS |
| ATTGACCCCAAGATCACAGA+GGG | 0.646842 | tig0020816:+74312 | None:intergenic |
| TGAGAATAGAAAGTGCATGA+CGG | 0.680398 | tig0020816:+74338 | None:intergenic |
| ATGTCCAATATACTCGACAC+GGG | 0.686297 | tig0020816:-74367 | Msa1386450:CDS |
| GATTGACCCCAAGATCACAG+AGG | 0.775929 | tig0020816:+74311 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTGTCAAGGAACTTAGAA+AGG | - | tig0020816:74374-74393 | Msa1386450:CDS | 30.0% | |
| ! | TGAGAATAGAAAGTGCATGA+CGG | + | tig0020816:74311-74330 | None:intergenic | 35.0% |
| TAGGATGCAATGATGACATT+GGG | + | tig0020816:74407-74426 | None:intergenic | 35.0% | |
| ! | AAGAAGTGCACGAGAATCTT+TGG | - | tig0020816:74242-74261 | Msa1386450:CDS | 40.0% |
| TATTGGACATGTGAAATGCC+AGG | + | tig0020816:74269-74288 | None:intergenic | 40.0% | |
| CATGTCCAATATACTCGACA+CGG | - | tig0020816:74278-74297 | Msa1386450:CDS | 40.0% | |
| ATGTCCAATATACTCGACAC+GGG | - | tig0020816:74279-74298 | Msa1386450:CDS | 40.0% | |
| TAAACCCGTGTCGAGTATAT+TGG | + | tig0020816:74286-74305 | None:intergenic | 40.0% | |
| TCAAGGAACTTAGAAAGGAG+AGG | - | tig0020816:74379-74398 | Msa1386450:CDS | 40.0% | |
| GTAGGATGCAATGATGACAT+TGG | + | tig0020816:74408-74427 | None:intergenic | 40.0% | |
| TCATTGCCCTCTGTGATCTT+GGG | - | tig0020816:74328-74347 | Msa1386450:CDS | 45.0% | |
| ATTGACCCCAAGATCACAGA+GGG | + | tig0020816:74337-74356 | None:intergenic | 45.0% | |
| ACAATAGCAGCAAGTGCTTC+AGG | + | tig0020816:74360-74379 | None:intergenic | 45.0% | |
| ! | AGCACTTGCTGCTATTGTCA+AGG | - | tig0020816:74362-74381 | Msa1386450:CDS | 45.0% |
| GTGCACTTCTTGCAGCTTCA+GGG | + | tig0020816:74233-74252 | None:intergenic | 50.0% | |
| TGCACGAGAATCTTTGGACC+TGG | - | tig0020816:74248-74267 | Msa1386450:CDS | 50.0% | |
| CTCATTGCCCTCTGTGATCT+TGG | - | tig0020816:74327-74346 | Msa1386450:CDS | 50.0% | |
| CATTGCCCTCTGTGATCTTG+GGG | - | tig0020816:74329-74348 | Msa1386450:CDS | 50.0% | |
| GATTGACCCCAAGATCACAG+AGG | + | tig0020816:74338-74357 | None:intergenic | 50.0% | |
| CGTGCACTTCTTGCAGCTTC+AGG | + | tig0020816:74234-74253 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0020816 | gene | 74225 | 74443 | 74225 | ID=Msa1386450;Name=Msa1386450 |
| tig0020816 | mRNA | 74225 | 74443 | 74225 | ID=Msa1386450-mRNA-1;Parent=Msa1386450;Name=Msa1386450-mRNA-1;_AED=0.40;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|72 |
| tig0020816 | exon | 74225 | 74443 | 74225 | ID=Msa1386450-mRNA-1:exon:10215;Parent=Msa1386450-mRNA-1 |
| tig0020816 | CDS | 74225 | 74443 | 74225 | ID=Msa1386450-mRNA-1:cds;Parent=Msa1386450-mRNA-1 |
| Gene Sequence |
| Protein sequence |
