AlfalfaGEDB | Msa0808180

M. sativa cultivar zhongmu-4 / Msa0808180

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0808180	RHN55861.1	71.351	185	29	1	1	161	86	270	1.06e-86	265

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0808180	A0A396HTK7	71.351	185	29	1	1	161	86	270	5.06e-87	265

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0754180	Msa0808180	0.828449	8.934046e-55	-8.615850e-47
Msa0784580	Msa0808180	0.811613	6.414129e-51	-8.615850e-47
Msa0808180	Msa0818010	0.870502	1.390520e-66	-8.615850e-47
Msa0808180	Msa0818030	0.800074	1.709198e-48	-8.615850e-47
Msa0808180	Msa1006250	0.820264	7.517607e-53	-8.615850e-47
Msa0808180	Msa1006400	0.806788	6.939722e-50	-8.615850e-47
Msa0808180	Msa1048490	0.846470	2.129103e-59	-8.615850e-47
Msa0808180	Msa1048610	0.821173	4.648921e-53	-8.615850e-47
Msa0808180	Msa1287560	0.814184	1.752867e-51	-8.615850e-47
Msa0808180	Msa1444370	0.838359	3.013320e-57	-8.615850e-47
Msa0142230	Msa0808180	0.802348	5.855215e-49	-8.615850e-47
Msa0235190	Msa0808180	0.802670	5.024438e-49	-8.615850e-47
Msa0458700	Msa0808180	0.804382	2.219054e-49	-8.615850e-47
Msa0273570	Msa0808180	0.835308	1.810314e-56	-8.615850e-47
Msa0331430	Msa0808180	0.828376	9.304048e-55	-8.615850e-47
Msa0533430	Msa0808180	0.819535	1.103585e-52	-8.615850e-47
Msa0580610	Msa0808180	0.836184	1.085631e-56	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0808180	MtrunA17_Chr5g0423041	71.351	185	29	1	1	161	86	270	9.75e-91	265
Msa0808180	MtrunA17_Chr5g0423061	46.111	180	59	9	1	158	83	246	3.60e-31	117

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 22 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTCTAATTCAGTAGAATTT+AGG	0.234340	5_3:+32006701	None:intergenic
AAGTTTGGCGTATTCTTTAT+TGG	0.235481	5_3:+32006679	None:intergenic
AACAATTCAGTTATCATCTA+TGG	0.307651	5_3:-32006724	Msa0808180:CDS
TGTGGAGGATGTTATCTTGC+AGG	0.333432	5_3:-32006427	Msa0808180:CDS
TTGAAAGTTTCAAACGGAAC+TGG	0.359531	5_3:+32006499	None:intergenic
CATTTGAAGAATGCCGTAAT+AGG	0.364617	5_3:-32006209	Msa0808180:CDS
TATATTTGAACTTGTTATCA+TGG	0.366422	5_3:+32006605	None:intergenic
GAGAACATGGGAGGAGAATC+AGG	0.385777	5_3:+32006544	None:intergenic
TTAAATTTCACCTGTGGCTC+AGG	0.398811	5_3:-32006376	Msa0808180:intron
AACTTCTATGTATGCAATCA+TGG	0.414576	5_3:-32006640	Msa0808180:CDS
ATAGTGTTAATGCTGAAGTT+TGG	0.453646	5_3:+32006664	None:intergenic
TTACAATACAGTACCTATTA+CGG	0.454583	5_3:+32006196	None:intergenic
CGTTCATTTCTCCTAGGAAT+CGG	0.463672	5_3:+32006233	None:intergenic
AAATGACGTTCATTTCTCCT+AGG	0.467381	5_3:+32006227	None:intergenic
TGCTAAAACCTCCGATTCCT+AGG	0.472473	5_3:-32006244	Msa0808180:CDS
AGGTGAAATTTAAAGTTTCG+TGG	0.494248	5_3:+32006386	None:intergenic
GGAACTGGGAAACAAGCTAA+TGG	0.513120	5_3:+32006514	None:intergenic
TGAAAGTTTCAAACGGAACT+GGG	0.527552	5_3:+32006500	None:intergenic
ACACAGTTGAAAGTTTCAAA+CGG	0.536755	5_3:+32006493	None:intergenic
AATGTGTTTACCTGAGCCAC+AGG	0.575075	5_3:+32006366	None:intergenic
GAAACTTTAAATTTCACCTG+TGG	0.617193	5_3:-32006382	Msa0808180:CDS
TCATTTCTCCTAGGAATCGG+AGG	0.618904	5_3:+32006236	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATATTTGAACTTGTTATCA+TGG	+	chr5_3:32006321-32006340	None:intergenic	20.0%
!!!	AAAATTGATTTAGAGTTTTG+TGG	-	chr5_3:32006478-32006497	Msa0808180:CDS	20.0%
!	AACAATTCAGTTATCATCTA+TGG	-	chr5_3:32006199-32006218	Msa0808180:CDS	25.0%
!	AATGGAAAAATAGAGAACAT+GGG	+	chr5_3:32006394-32006413	None:intergenic	25.0%
!	TAATGGAAAAATAGAGAACA+TGG	+	chr5_3:32006395-32006414	None:intergenic	25.0%
!	TTACAATACAGTACCTATTA+CGG	+	chr5_3:32006730-32006749	None:intergenic	25.0%
	GCTCTAATTCAGTAGAATTT+AGG	+	chr5_3:32006225-32006244	None:intergenic	30.0%
	AAGTTTGGCGTATTCTTTAT+TGG	+	chr5_3:32006247-32006266	None:intergenic	30.0%
	ATAGTGTTAATGCTGAAGTT+TGG	+	chr5_3:32006262-32006281	None:intergenic	30.0%
	AACTTCTATGTATGCAATCA+TGG	-	chr5_3:32006283-32006302	Msa0808180:CDS	30.0%
	ACACAGTTGAAAGTTTCAAA+CGG	+	chr5_3:32006433-32006452	None:intergenic	30.0%
!!	ATTGATTTAGAGTTTTGTGG+AGG	-	chr5_3:32006481-32006500	Msa0808180:CDS	30.0%
!	AGGTGAAATTTAAAGTTTCG+TGG	+	chr5_3:32006540-32006559	None:intergenic	30.0%
	GAAACTTTAAATTTCACCTG+TGG	-	chr5_3:32006541-32006560	Msa0808180:CDS	30.0%
!!	ATTTGTTGTACGAGATTGTA+TGG	-	chr5_3:32006601-32006620	Msa0808180:CDS	30.0%
	TGAAAGTTTCAAACGGAACT+GGG	+	chr5_3:32006426-32006445	None:intergenic	35.0%
	TTGAAAGTTTCAAACGGAAC+TGG	+	chr5_3:32006427-32006446	None:intergenic	35.0%
	TACAACAAATGAATGAGCGT+CGG	+	chr5_3:32006591-32006610	None:intergenic	35.0%
	AAATGACGTTCATTTCTCCT+AGG	+	chr5_3:32006699-32006718	None:intergenic	35.0%
	CATTTGAAGAATGCCGTAAT+AGG	-	chr5_3:32006714-32006733	Msa0808180:CDS	35.0%
	GGAAAAATAGAGAACATGGG+AGG	+	chr5_3:32006391-32006410	None:intergenic	40.0%
	TTAAATTTCACCTGTGGCTC+AGG	-	chr5_3:32006547-32006566	Msa0808180:CDS	40.0%
	CGTTCATTTCTCCTAGGAAT+CGG	+	chr5_3:32006693-32006712	None:intergenic	40.0%
	GGAACTGGGAAACAAGCTAA+TGG	+	chr5_3:32006412-32006431	None:intergenic	45.0%
	TGTGGAGGATGTTATCTTGC+AGG	-	chr5_3:32006496-32006515	Msa0808180:CDS	45.0%
	AATGTGTTTACCTGAGCCAC+AGG	+	chr5_3:32006560-32006579	None:intergenic	45.0%
	TGCTAAAACCTCCGATTCCT+AGG	-	chr5_3:32006679-32006698	Msa0808180:CDS	45.0%
	TCATTTCTCCTAGGAATCGG+AGG	+	chr5_3:32006690-32006709	None:intergenic	45.0%
	GAGAACATGGGAGGAGAATC+AGG	+	chr5_3:32006382-32006401	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	32006196	32006749	32006196	ID=Msa0808180;Name=Msa0808180
chr5_3	mRNA	32006196	32006749	32006196	ID=Msa0808180-mRNA-1;Parent=Msa0808180;Name=Msa0808180-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0\|0\|1\|1\|1\|2\|0\|161
chr5_3	exon	32006377	32006749	32006377	ID=Msa0808180-mRNA-1:exon:10567;Parent=Msa0808180-mRNA-1
chr5_3	exon	32006196	32006308	32006196	ID=Msa0808180-mRNA-1:exon:10566;Parent=Msa0808180-mRNA-1
chr5_3	CDS	32006377	32006749	32006377	ID=Msa0808180-mRNA-1:cds;Parent=Msa0808180-mRNA-1
chr5_3	CDS	32006196	32006308	32006196	ID=Msa0808180-mRNA-1:cds;Parent=Msa0808180-mRNA-1

Gene Sequence

>Msa0808180

ATGAACAATTCAGTTATCATCTATGGCCTAAATTCTACTGAATTAGAGCCAATAAAGAATACGCCAAACTTCAGCATTAACACTATCAACTTCTATGTATGCAATCATGGCTATGACAATATCCATGATAACAAGTTCAAATATAGAAGCTGTTCTGATTATGATATCTACTTTACTTTTGCACCTGATTCTCCTCCCATGTTCTCTATTTTTCCATTAGCTTGTTTCCCAGTTCCGTTTGAAACTTTCAACTGTGTAGAAATAATTTCATTACTCGAAATTAAAATTGATTTAGAGTTTTGTGGAGGATGTTATCTTGCAGGAAAGTCATGTCTACTAAACCACGAAACTTTAAATTTCACCTGTGGCTCAGACAATGTACACTCATCGTTCTCTTACAACTTCTGCTATGTAGTGCTAAAACCTCCGATTCCTAGGAGAAATGAACGTCATTTGAAGAATGCCGTAATAGGTACTGTATTGTAA

Protein sequence

>Msa0808180

MNNSVIIYGLNSTELEPIKNTPNFSINTINFYVCNHGYDNIHDNKFKYRSCSDYDIYFTFAPDSPPMFSIFPLACFPVPFETFNCVEIISLLEIKIDLEFCGGCYLAGKSCLLNHETLNFTCGSDNVHSSFSYNFCYVVLKPPIPRRNERHLKNAVIGTVL*