Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754180 | sp|A9QY40|SBT1_LOTJA | 60.563 | 71 | 28 | 0 | 2 | 72 | 679 | 749 | 1.89e-21 | 89.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754180 | G7KEU3 | 91.781 | 73 | 6 | 0 | 1 | 73 | 686 | 758 | 1.01e-35 | 137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0021460 | Msa0754180 | 0.805254 | 1.458761e-49 | -8.615850e-47 |
| Msa0055690 | Msa0754180 | 0.816297 | 5.947514e-52 | -8.615850e-47 |
| Msa0109990 | Msa0754180 | 0.803209 | 3.887806e-49 | -8.615850e-47 |
| Msa0629850 | Msa0754180 | 0.818082 | 2.359419e-52 | -8.615850e-47 |
| Msa0629930 | Msa0754180 | 0.812529 | 4.049947e-51 | -8.615850e-47 |
| Msa0650280 | Msa0754180 | 0.815296 | 9.939638e-52 | -8.615850e-47 |
| Msa0754180 | Msa0773610 | 0.803632 | 3.177485e-49 | -8.615850e-47 |
| Msa0754180 | Msa0784580 | 0.861949 | 7.161163e-64 | -8.615850e-47 |
| Msa0754180 | Msa0808180 | 0.828449 | 8.934046e-55 | -8.615850e-47 |
| Msa0754180 | Msa0815320 | 0.812403 | 4.315862e-51 | -8.615850e-47 |
| Msa0754180 | Msa0818010 | 0.862238 | 5.836308e-64 | -8.615850e-47 |
| Msa0754180 | Msa0818030 | 0.823975 | 1.037031e-53 | -8.615850e-47 |
| Msa0754180 | Msa0858360 | 0.825053 | 5.781813e-54 | -8.615850e-47 |
| Msa0754180 | Msa0883370 | 0.805569 | 1.252848e-49 | -8.615850e-47 |
| Msa0754180 | Msa0912370 | 0.818159 | 2.266992e-52 | -8.615850e-47 |
| Msa0754180 | Msa0912500 | 0.807139 | 5.849603e-50 | -8.615850e-47 |
| Msa0754180 | Msa1006250 | 0.836818 | 7.488607e-57 | -8.615850e-47 |
| Msa0754180 | Msa1006400 | 0.824219 | 9.086231e-54 | -8.615850e-47 |
| Msa0754180 | Msa1006440 | 0.803483 | 3.412142e-49 | -8.615850e-47 |
| Msa0754180 | Msa1006680 | 0.805165 | 1.522686e-49 | -8.615850e-47 |
| Msa0754180 | Msa1009270 | 0.811500 | 6.786184e-51 | -8.615850e-47 |
| Msa0754180 | Msa1027240 | 0.834585 | 2.752368e-56 | -8.615850e-47 |
| Msa0754180 | Msa1048510 | 0.814232 | 1.710626e-51 | -8.615850e-47 |
| Msa0754180 | Msa1048590 | 0.843453 | 1.388685e-58 | -8.615850e-47 |
| Msa0754180 | Msa1048610 | 0.851665 | 7.653162e-61 | -8.615850e-47 |
| Msa0754180 | Msa1050480 | 0.830012 | 3.731773e-55 | -8.615850e-47 |
| Msa0754180 | Msa1050490 | 0.814611 | 1.410508e-51 | -8.615850e-47 |
| Msa0754180 | Msa1050500 | 0.830073 | 3.606819e-55 | -8.615850e-47 |
| Msa0754180 | Msa1100180 | 0.833158 | 6.261409e-56 | -8.615850e-47 |
| Msa0754180 | Msa1135660 | 0.801000 | 1.106883e-48 | -8.615850e-47 |
| Msa0754180 | Msa1198090 | 0.817073 | 3.984636e-52 | -8.615850e-47 |
| Msa0754180 | Msa1272480 | 0.800678 | 1.287631e-48 | -8.615850e-47 |
| Msa0754180 | Msa1279850 | 0.807394 | 5.164830e-50 | -8.615850e-47 |
| Msa0754180 | Msa1285840 | 0.828795 | 7.370030e-55 | -8.615850e-47 |
| Msa0754180 | Msa1285850 | 0.804021 | 2.638138e-49 | -8.615850e-47 |
| Msa0754180 | Msa1287560 | 0.825584 | 4.329563e-54 | -8.615850e-47 |
| Msa0754180 | Msa1288000 | 0.806149 | 9.463582e-50 | -8.615850e-47 |
| Msa0754180 | Msa1359820 | 0.822049 | 2.916760e-53 | -8.615850e-47 |
| Msa0754180 | Msa1384320 | 0.835972 | 1.228919e-56 | -8.615850e-47 |
| Msa0754180 | Msa1406600 | 0.800124 | 1.669826e-48 | -8.615850e-47 |
| Msa0754180 | Msa1423990 | 0.842957 | 1.881976e-58 | -8.615850e-47 |
| Msa0754180 | Msa1444370 | 0.858083 | 1.050888e-62 | -8.615850e-47 |
| Msa0142230 | Msa0754180 | 0.830526 | 2.794874e-55 | -8.615850e-47 |
| Msa0156170 | Msa0754180 | 0.816902 | 4.352484e-52 | -8.615850e-47 |
| Msa0156190 | Msa0754180 | 0.801232 | 9.921888e-49 | -8.615850e-47 |
| Msa0203070 | Msa0754180 | 0.810498 | 1.118671e-50 | -8.615850e-47 |
| Msa0211680 | Msa0754180 | 0.828743 | 7.587458e-55 | -8.615850e-47 |
| Msa0212210 | Msa0754180 | 0.807765 | 4.308542e-50 | -8.615850e-47 |
| Msa0235190 | Msa0754180 | 0.820973 | 5.168020e-53 | -8.615850e-47 |
| Msa0389310 | Msa0754180 | 0.830249 | 3.265810e-55 | -8.615850e-47 |
| Msa0458700 | Msa0754180 | 0.850701 | 1.431929e-60 | -8.615850e-47 |
| Msa0273560 | Msa0754180 | 0.839997 | 1.133270e-57 | -8.615850e-47 |
| Msa0273570 | Msa0754180 | 0.834008 | 3.841309e-56 | -8.615850e-47 |
| Msa0284810 | Msa0754180 | 0.821946 | 3.081016e-53 | -8.615850e-47 |
| Msa0313780 | Msa0754180 | 0.805990 | 1.022394e-49 | -8.615850e-47 |
| Msa0313790 | Msa0754180 | 0.815993 | 6.952669e-52 | -8.615850e-47 |
| Msa0313920 | Msa0754180 | 0.804420 | 2.178516e-49 | -8.615850e-47 |
| Msa0323550 | Msa0754180 | 0.819116 | 1.374890e-52 | -8.615850e-47 |
| Msa0331430 | Msa0754180 | 0.832495 | 9.149467e-56 | -8.615850e-47 |
| Msa0352010 | Msa0754180 | 0.802875 | 4.558081e-49 | -8.615850e-47 |
| Msa0499710 | Msa0754180 | 0.802080 | 6.647607e-49 | -8.615850e-47 |
| Msa0533430 | Msa0754180 | 0.860773 | 1.635176e-63 | -8.615850e-47 |
| Msa0555730 | Msa0754180 | 0.823332 | 1.466465e-53 | -8.615850e-47 |
| Msa0580610 | Msa0754180 | 0.859848 | 3.113577e-63 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754180 | MtrunA17_Chr5g0398331 | 91.781 | 73 | 6 | 0 | 1 | 73 | 681 | 753 | 2.04e-39 | 137 |
| Msa0754180 | MtrunA17_Chr5g0398311 | 69.444 | 72 | 22 | 0 | 2 | 73 | 678 | 749 | 1.16e-27 | 103 |
| Msa0754180 | MtrunA17_Chr5g0398241 | 55.556 | 72 | 32 | 0 | 2 | 73 | 685 | 756 | 3.50e-22 | 88.2 |
| Msa0754180 | MtrunA17_Chr5g0398401 | 56.338 | 71 | 31 | 0 | 2 | 72 | 685 | 755 | 7.70e-22 | 87.0 |
| Msa0754180 | MtrunA17_Chr5g0398351 | 53.521 | 71 | 33 | 0 | 2 | 72 | 1436 | 1506 | 2.25e-20 | 82.8 |
| Msa0754180 | MtrunA17_Chr5g0398351 | 52.113 | 71 | 34 | 0 | 2 | 72 | 665 | 735 | 2.84e-20 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTCAGTGAATGTTATT+TGG | 0.150529 | 5_2:+7730575 | None:intergenic |
| GGGTCTATCAAGTGGGTTTC+TGG | 0.162766 | 5_2:-7730478 | Msa0754180:CDS |
| CTTCTCAGTGAATGTTATTT+GGG | 0.286362 | 5_2:+7730576 | None:intergenic |
| CACACAAAACTGACAGATAT+AGG | 0.294764 | 5_2:+7730436 | None:intergenic |
| ACACAAAACTGACAGATATA+GGG | 0.386335 | 5_2:+7730437 | None:intergenic |
| TGTCGAAATTGATGTTCCTT+TGG | 0.438005 | 5_2:-7730623 | Msa0754180:CDS |
| TCAGTGAATGTTATTTGGGC+AGG | 0.458141 | 5_2:+7730580 | None:intergenic |
| AGGTGATAACGAAATTGCTC+AGG | 0.485219 | 5_2:-7730500 | Msa0754180:CDS |
| CCCGAAAACATAGAGAATAG+AGG | 0.554483 | 5_2:-7730520 | Msa0754180:CDS |
| TGCTCAGGGGTCTATCAAGT+GGG | 0.583423 | 5_2:-7730485 | Msa0754180:CDS |
| TTGCTCAGGGGTCTATCAAG+TGG | 0.591916 | 5_2:-7730486 | Msa0754180:CDS |
| GGTGATAACGAAATTGCTCA+GGG | 0.601427 | 5_2:-7730499 | Msa0754180:CDS |
| ATACTGATATCAACAGCCAA+AGG | 0.644765 | 5_2:+7730607 | None:intergenic |
| GTGATAACGAAATTGCTCAG+GGG | 0.778556 | 5_2:-7730498 | Msa0754180:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTCTCAGTGAATGTTATTT+GGG | + | chr5_2:7730496-7730515 | None:intergenic | 30.0% |
| TCTTCTCAGTGAATGTTATT+TGG | + | chr5_2:7730497-7730516 | None:intergenic | 30.0% | |
| ! | ACCTCTATTCTCTATGTTTT+CGG | + | chr5_2:7730553-7730572 | None:intergenic | 30.0% |
| ACACAAAACTGACAGATATA+GGG | + | chr5_2:7730635-7730654 | None:intergenic | 30.0% | |
| TGTCGAAATTGATGTTCCTT+TGG | - | chr5_2:7730446-7730465 | Msa0754180:CDS | 35.0% | |
| ATACTGATATCAACAGCCAA+AGG | + | chr5_2:7730465-7730484 | None:intergenic | 35.0% | |
| ! | CCTCTATTCTCTATGTTTTC+GGG | + | chr5_2:7730552-7730571 | None:intergenic | 35.0% |
| CACACAAAACTGACAGATAT+AGG | + | chr5_2:7730636-7730655 | None:intergenic | 35.0% | |
| ! | TCAGTGAATGTTATTTGGGC+AGG | + | chr5_2:7730492-7730511 | None:intergenic | 40.0% |
| GAAGCAAAAAGCTGCATACT+CGG | - | chr5_2:7730515-7730534 | Msa0754180:CDS | 40.0% | |
| CCCGAAAACATAGAGAATAG+AGG | - | chr5_2:7730549-7730568 | Msa0754180:CDS | 40.0% | |
| ! | AGGTGATAACGAAATTGCTC+AGG | - | chr5_2:7730569-7730588 | Msa0754180:CDS | 40.0% |
| ! | GGTGATAACGAAATTGCTCA+GGG | - | chr5_2:7730570-7730589 | Msa0754180:CDS | 40.0% |
| ! | GTGATAACGAAATTGCTCAG+GGG | - | chr5_2:7730571-7730590 | Msa0754180:CDS | 40.0% |
| TTGCTCAGGGGTCTATCAAG+TGG | - | chr5_2:7730583-7730602 | Msa0754180:CDS | 50.0% | |
| TGCTCAGGGGTCTATCAAGT+GGG | - | chr5_2:7730584-7730603 | Msa0754180:CDS | 50.0% | |
| ! | GGGTCTATCAAGTGGGTTTC+TGG | - | chr5_2:7730591-7730610 | Msa0754180:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 7730435 | 7730656 | 7730435 | ID=Msa0754180;Name=Msa0754180 |
| chr5_2 | mRNA | 7730435 | 7730656 | 7730435 | ID=Msa0754180-mRNA-1;Parent=Msa0754180;Name=Msa0754180-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|73 |
| chr5_2 | exon | 7730435 | 7730656 | 7730435 | ID=Msa0754180-mRNA-1:exon:3537;Parent=Msa0754180-mRNA-1 |
| chr5_2 | CDS | 7730435 | 7730656 | 7730435 | ID=Msa0754180-mRNA-1:cds;Parent=Msa0754180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
