AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0910660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0910660 MtrunA17_Chr1g0201251 69.231 52 15 1 1 52 86 136 1.28e-17 75.1
Msa0910660 MtrunA17_Chr6g0466891 63.462 52 18 1 1 52 86 136 2.02e-16 72.0
Msa0910660 MtrunA17_Chr3g0090241 72.340 47 12 1 1 47 86 131 1.06e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0910660 AT1G07140.1 63.462 52 18 1 1 52 89 139 1.75e-14 67.4
Msa0910660 AT2G30060.1 65.957 47 15 1 1 47 91 136 3.56e-13 63.5
Msa0910660 AT5G58590.1 59.615 52 20 1 1 52 88 138 1.82e-12 61.6

Find 38 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATATGTTGGTGTGCTTTC+TGG 0.146976 6_2:-15474983 Msa0910660:CDS
ACAACCGAGCCGTACTAAAT+TGG 0.252478 6_2:+15475893 None:intergenic
GCTGCTTCAACTGTGTTTGC+AGG 0.266451 6_2:-15475007 Msa0910660:CDS
CCGAGAGAGTAGGGTGTATT+TGG 0.267050 6_2:-15475273 Msa0910660:intron
AGCGCAGATCTTGAGGGTTT+TGG 0.290367 6_2:+15475767 None:intergenic
CAGTTCTGTTCGAGGAAGTT+GGG 0.316931 6_2:-15475098 Msa0910660:CDS
ATAAGGTTACTTGAAAAGTT+AGG 0.336456 6_2:-15475806 Msa0910660:five_prime_UTR
GATTCGGTTGCTAGCAGTTT+GGG 0.375277 6_2:-15475029 Msa0910660:CDS
TGATTCGGTTGCTAGCAGTT+TGG 0.405660 6_2:-15475030 Msa0910660:CDS
CGATGGAGTTCTTAAGCATA+AGG 0.412330 6_2:-15475823 Msa0910660:five_prime_UTR
GCAGTTCTGTTCGAGGAAGT+TGG 0.425432 6_2:-15475099 Msa0910660:CDS
GAATTGAGAAATCGTGTGTT+TGG 0.437554 6_2:-15475601 Msa0910660:CDS
CTATCTTGTTAAAGTGGTGT+TGG 0.446382 6_2:-15475299 Msa0910660:CDS
GAAAAGTTAGGCTTCTTATG+AGG 0.446917 6_2:-15475794 Msa0910660:exon
TAACTTTATGAGTTCTTGAC+TGG 0.460076 6_2:-15475848 Msa0910660:five_prime_UTR
AATCGTGTGTTTGGCATGCT+AGG 0.466273 6_2:-15475592 Msa0910660:CDS
CTGCTTCAACTGTGTTTGCA+GGG 0.486018 6_2:-15475006 Msa0910660:CDS
CGAGGAAGTTGGGGTCTAGT+TGG 0.486820 6_2:-15475088 Msa0910660:CDS
ACGATTAGCGCAGATCTTGA+GGG 0.489998 6_2:+15475761 None:intergenic
TTCTCTTGCATACTCATCAT+AGG 0.492591 6_2:+15475629 None:intergenic
GACGATTAGCGCAGATCTTG+AGG 0.502484 6_2:+15475760 None:intergenic
TCTGCCATGAGATGGTGATT+CGG 0.523598 6_2:-15475045 Msa0910660:CDS
CATGCTAGGGATTCTGCTGA+TGG 0.533927 6_2:-15475578 Msa0910660:intron
TGATAACTATCTTGTTAAAG+TGG 0.534578 6_2:-15475305 Msa0910660:CDS
ATCGTGTGTTTGGCATGCTA+GGG 0.537370 6_2:-15475591 Msa0910660:CDS
TGTTGGAATCCGAGAGAGTA+GGG 0.545544 6_2:-15475282 Msa0910660:CDS
CCAAATACACCCTACTCTCT+CGG 0.552355 6_2:+15475273 None:intergenic
ATGAGTATGCAAGAGAATGT+TGG 0.553283 6_2:-15475623 Msa0910660:CDS
TGAGTTCTTGACTGGTACGA+TGG 0.554894 6_2:-15475840 Msa0910660:five_prime_UTR
TGCTTCAACTGTGTTTGCAG+GGG 0.555834 6_2:-15475005 Msa0910660:CDS
AGTTCTGTTCGAGGAAGTTG+GGG 0.577830 6_2:-15475097 Msa0910660:CDS
GTGTTGGAATCCGAGAGAGT+AGG 0.584549 6_2:-15475283 Msa0910660:CDS
AGCAAAGATATTAGCTGCGG+AGG 0.590198 6_2:-15475918 None:intergenic
GCGGAGGATCCAATTTAGTA+CGG 0.590877 6_2:-15475902 Msa0910660:five_prime_UTR
GGATCCAATTTAGTACGGCT+CGG 0.591535 6_2:-15475897 Msa0910660:five_prime_UTR
GTGTTTGCAGGGGTATATGT+TGG 0.608820 6_2:-15474995 Msa0910660:CDS
AGCAGCAAAGATATTAGCTG+CGG 0.638123 6_2:-15475921 None:intergenic
GCAACCGAATCACCATCTCA+TGG 0.649274 6_2:+15475041 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTAGATGATGTTTTATTT+TGG - chr6_2:15475442-15475461 Msa0910660:intron 20.0%
!!! GTTAGATGATGTTTTATTTT+GGG - chr6_2:15475443-15475462 Msa0910660:intron 20.0%
!!! TTAGATGATGTTTTATTTTG+GGG - chr6_2:15475444-15475463 Msa0910660:intron 20.0%
!!! TGTTAGATGATGTTTTATTT+TGG - chr6_2:15475442-15475461 Msa0910660:intron 20.0%
!!! GTTAGATGATGTTTTATTTT+GGG - chr6_2:15475443-15475462 Msa0910660:intron 20.0%
!!! TTAGATGATGTTTTATTTTG+GGG - chr6_2:15475444-15475463 Msa0910660:intron 20.0%
! ATAAGGTTACTTGAAAAGTT+AGG - chr6_2:15475067-15475086 Msa0910660:CDS 25.0%
!!! TTTTGTGATTTTGATTGTTG+TGG - chr6_2:15475198-15475217 Msa0910660:intron 25.0%
!!! CAGATTTTTTGTAGTTAGAT+TGG - chr6_2:15475396-15475415 Msa0910660:intron 25.0%
!! ATTGGTTTAGATGAATATTG+TGG - chr6_2:15475414-15475433 Msa0910660:intron 25.0%
! TTGGTTTAGATGAATATTGT+GGG - chr6_2:15475415-15475434 Msa0910660:intron 25.0%
!!! TAGATGATGTTTTATTTTGG+GGG - chr6_2:15475445-15475464 Msa0910660:intron 25.0%
! TGATAACTATCTTGTTAAAG+TGG - chr6_2:15475568-15475587 Msa0910660:intron 25.0%
! AAATGAGTAAAATTACAGAC+CGG + chr6_2:15475661-15475680 None:intergenic 25.0%
! GTTTACTTATCCAAAAAGAT+AGG - chr6_2:15475693-15475712 Msa0910660:intron 25.0%
!! AGGAAAAATTCCTATCTTTT+TGG + chr6_2:15475706-15475725 None:intergenic 25.0%
! ATAAGGTTACTTGAAAAGTT+AGG - chr6_2:15475067-15475086 Msa0910660:CDS 25.0%
!!! TTTTGTGATTTTGATTGTTG+TGG - chr6_2:15475198-15475217 Msa0910660:intron 25.0%
!!! CAGATTTTTTGTAGTTAGAT+TGG - chr6_2:15475396-15475415 Msa0910660:intron 25.0%
!! ATTGGTTTAGATGAATATTG+TGG - chr6_2:15475414-15475433 Msa0910660:intron 25.0%
! TTGGTTTAGATGAATATTGT+GGG - chr6_2:15475415-15475434 Msa0910660:intron 25.0%
!!! TAGATGATGTTTTATTTTGG+GGG - chr6_2:15475445-15475464 Msa0910660:intron 25.0%
! TGATAACTATCTTGTTAAAG+TGG - chr6_2:15475568-15475587 Msa0910660:intron 25.0%
! AAATGAGTAAAATTACAGAC+CGG + chr6_2:15475661-15475680 None:intergenic 25.0%
! GTTTACTTATCCAAAAAGAT+AGG - chr6_2:15475693-15475712 Msa0910660:intron 25.0%
!! AGGAAAAATTCCTATCTTTT+TGG + chr6_2:15475706-15475725 None:intergenic 25.0%
TAACTTTATGAGTTCTTGAC+TGG - chr6_2:15475025-15475044 Msa0910660:CDS 30.0%
! AAGATGCACTTTTCTGTTTT+CGG - chr6_2:15475327-15475346 Msa0910660:intron 30.0%
! AAGATAGGAATTTTTCCTTG+TGG - chr6_2:15475708-15475727 Msa0910660:intron 30.0%
AAAGGAAAATATGTACCACA+AGG + chr6_2:15475726-15475745 None:intergenic 30.0%
! TTGTGGTACATATTTTCCTT+TGG - chr6_2:15475725-15475744 Msa0910660:intron 30.0%
TAACTTTATGAGTTCTTGAC+TGG - chr6_2:15475025-15475044 Msa0910660:CDS 30.0%
! AAGATGCACTTTTCTGTTTT+CGG - chr6_2:15475327-15475346 Msa0910660:intron 30.0%
! AAGATAGGAATTTTTCCTTG+TGG - chr6_2:15475708-15475727 Msa0910660:intron 30.0%
AAAGGAAAATATGTACCACA+AGG + chr6_2:15475726-15475745 None:intergenic 30.0%
! TTGTGGTACATATTTTCCTT+TGG - chr6_2:15475725-15475744 Msa0910660:intron 30.0%
GAAAAGTTAGGCTTCTTATG+AGG - chr6_2:15475079-15475098 Msa0910660:CDS 35.0%
TTCTCTTGCATACTCATCAT+AGG + chr6_2:15475247-15475266 None:intergenic 35.0%
ATGAGTATGCAAGAGAATGT+TGG - chr6_2:15475250-15475269 Msa0910660:intron 35.0%
GAATTGAGAAATCGTGTGTT+TGG - chr6_2:15475272-15475291 Msa0910660:intron 35.0%
ACTCAACAACTATTGACATG+TGG - chr6_2:15475513-15475532 Msa0910660:intron 35.0%
CTATCTTGTTAAAGTGGTGT+TGG - chr6_2:15475574-15475593 Msa0910660:intron 35.0%
! TTTCCTTTGGTAATTGTAGC+AGG - chr6_2:15475738-15475757 Msa0910660:intron 35.0%
! TTCCTTTGGTAATTGTAGCA+GGG - chr6_2:15475739-15475758 Msa0910660:intron 35.0%
GAAAAGTTAGGCTTCTTATG+AGG - chr6_2:15475079-15475098 Msa0910660:CDS 35.0%
TTCTCTTGCATACTCATCAT+AGG + chr6_2:15475247-15475266 None:intergenic 35.0%
ATGAGTATGCAAGAGAATGT+TGG - chr6_2:15475250-15475269 Msa0910660:intron 35.0%
GAATTGAGAAATCGTGTGTT+TGG - chr6_2:15475272-15475291 Msa0910660:intron 35.0%
ACTCAACAACTATTGACATG+TGG - chr6_2:15475513-15475532 Msa0910660:intron 35.0%
CTATCTTGTTAAAGTGGTGT+TGG - chr6_2:15475574-15475593 Msa0910660:intron 35.0%
! TTTCCTTTGGTAATTGTAGC+AGG - chr6_2:15475738-15475757 Msa0910660:intron 35.0%
! TTCCTTTGGTAATTGTAGCA+GGG - chr6_2:15475739-15475758 Msa0910660:intron 35.0%
CGATGGAGTTCTTAAGCATA+AGG - chr6_2:15475050-15475069 Msa0910660:CDS 40.0%
AAATTACAGACCGGACAAGA+TGG + chr6_2:15475652-15475671 None:intergenic 40.0%
!!! CAGTTTTTTCTGCCATGAGA+TGG - chr6_2:15475820-15475839 Msa0910660:five_prime_UTR 40.0%
GTATATGTTGGTGTGCTTTC+TGG - chr6_2:15475890-15475909 Msa0910660:five_prime_UTR 40.0%
CGATGGAGTTCTTAAGCATA+AGG - chr6_2:15475050-15475069 Msa0910660:CDS 40.0%
AAATTACAGACCGGACAAGA+TGG + chr6_2:15475652-15475671 None:intergenic 40.0%
!!! CAGTTTTTTCTGCCATGAGA+TGG - chr6_2:15475820-15475839 Msa0910660:five_prime_UTR 40.0%
GTATATGTTGGTGTGCTTTC+TGG - chr6_2:15475890-15475909 Msa0910660:five_prime_UTR 40.0%
GCGGAGGATCCAATTTAGTA+CGG - chr6_2:15474971-15474990 Msa0910660:CDS 45.0%
GGATCCAATTTAGTACGGCT+CGG - chr6_2:15474976-15474995 Msa0910660:CDS 45.0%
ACAACCGAGCCGTACTAAAT+TGG + chr6_2:15474983-15475002 None:intergenic 45.0%
TGAGTTCTTGACTGGTACGA+TGG - chr6_2:15475033-15475052 Msa0910660:CDS 45.0%
ACGATTAGCGCAGATCTTGA+GGG + chr6_2:15475115-15475134 None:intergenic 45.0%
AATCGTGTGTTTGGCATGCT+AGG - chr6_2:15475281-15475300 Msa0910660:CDS 45.0%
ATCGTGTGTTTGGCATGCTA+GGG - chr6_2:15475282-15475301 Msa0910660:CDS 45.0%
TGTTGGAATCCGAGAGAGTA+GGG - chr6_2:15475591-15475610 Msa0910660:CDS 45.0%
CCAAATACACCCTACTCTCT+CGG + chr6_2:15475603-15475622 None:intergenic 45.0%
CACCCTGCTACAATTACCAA+AGG + chr6_2:15475744-15475763 None:intergenic 45.0%
! GTCTTTTGCAGTTCTGTTCG+AGG - chr6_2:15475767-15475786 Msa0910660:CDS 45.0%
CAGTTCTGTTCGAGGAAGTT+GGG - chr6_2:15475775-15475794 Msa0910660:CDS 45.0%
AGTTCTGTTCGAGGAAGTTG+GGG - chr6_2:15475776-15475795 Msa0910660:CDS 45.0%
TCTGCCATGAGATGGTGATT+CGG - chr6_2:15475828-15475847 Msa0910660:five_prime_UTR 45.0%
!! TGATTCGGTTGCTAGCAGTT+TGG - chr6_2:15475843-15475862 Msa0910660:five_prime_UTR 45.0%
! GATTCGGTTGCTAGCAGTTT+GGG - chr6_2:15475844-15475863 Msa0910660:five_prime_UTR 45.0%
CTGCTTCAACTGTGTTTGCA+GGG - chr6_2:15475867-15475886 Msa0910660:five_prime_UTR 45.0%
TGCTTCAACTGTGTTTGCAG+GGG - chr6_2:15475868-15475887 Msa0910660:five_prime_UTR 45.0%
! GTGTTTGCAGGGGTATATGT+TGG - chr6_2:15475878-15475897 Msa0910660:five_prime_UTR 45.0%
GCGGAGGATCCAATTTAGTA+CGG - chr6_2:15474971-15474990 Msa0910660:CDS 45.0%
GGATCCAATTTAGTACGGCT+CGG - chr6_2:15474976-15474995 Msa0910660:CDS 45.0%
ACAACCGAGCCGTACTAAAT+TGG + chr6_2:15474983-15475002 None:intergenic 45.0%
TGAGTTCTTGACTGGTACGA+TGG - chr6_2:15475033-15475052 Msa0910660:CDS 45.0%
ACGATTAGCGCAGATCTTGA+GGG + chr6_2:15475115-15475134 None:intergenic 45.0%
AATCGTGTGTTTGGCATGCT+AGG - chr6_2:15475281-15475300 Msa0910660:CDS 45.0%
ATCGTGTGTTTGGCATGCTA+GGG - chr6_2:15475282-15475301 Msa0910660:CDS 45.0%
TGTTGGAATCCGAGAGAGTA+GGG - chr6_2:15475591-15475610 Msa0910660:CDS 45.0%
CCAAATACACCCTACTCTCT+CGG + chr6_2:15475603-15475622 None:intergenic 45.0%
CACCCTGCTACAATTACCAA+AGG + chr6_2:15475744-15475763 None:intergenic 45.0%
! GTCTTTTGCAGTTCTGTTCG+AGG - chr6_2:15475767-15475786 Msa0910660:CDS 45.0%
CAGTTCTGTTCGAGGAAGTT+GGG - chr6_2:15475775-15475794 Msa0910660:CDS 45.0%
AGTTCTGTTCGAGGAAGTTG+GGG - chr6_2:15475776-15475795 Msa0910660:CDS 45.0%
TCTGCCATGAGATGGTGATT+CGG - chr6_2:15475828-15475847 Msa0910660:five_prime_UTR 45.0%
!! TGATTCGGTTGCTAGCAGTT+TGG - chr6_2:15475843-15475862 Msa0910660:five_prime_UTR 45.0%
! GATTCGGTTGCTAGCAGTTT+GGG - chr6_2:15475844-15475863 Msa0910660:five_prime_UTR 45.0%
CTGCTTCAACTGTGTTTGCA+GGG - chr6_2:15475867-15475886 Msa0910660:five_prime_UTR 45.0%
TGCTTCAACTGTGTTTGCAG+GGG - chr6_2:15475868-15475887 Msa0910660:five_prime_UTR 45.0%
! GTGTTTGCAGGGGTATATGT+TGG - chr6_2:15475878-15475897 Msa0910660:five_prime_UTR 45.0%
! AGCGCAGATCTTGAGGGTTT+TGG + chr6_2:15475109-15475128 None:intergenic 50.0%
GACGATTAGCGCAGATCTTG+AGG + chr6_2:15475116-15475135 None:intergenic 50.0%
CATGCTAGGGATTCTGCTGA+TGG - chr6_2:15475295-15475314 Msa0910660:CDS 50.0%
GTGTTGGAATCCGAGAGAGT+AGG - chr6_2:15475590-15475609 Msa0910660:CDS 50.0%
CCGAGAGAGTAGGGTGTATT+TGG - chr6_2:15475600-15475619 Msa0910660:CDS 50.0%
GTTGCACATGCCATCTTGTC+CGG - chr6_2:15475639-15475658 Msa0910660:intron 50.0%
GCAGTTCTGTTCGAGGAAGT+TGG - chr6_2:15475774-15475793 Msa0910660:CDS 50.0%
GCAACCGAATCACCATCTCA+TGG + chr6_2:15475835-15475854 None:intergenic 50.0%
GCTGCTTCAACTGTGTTTGC+AGG - chr6_2:15475866-15475885 Msa0910660:five_prime_UTR 50.0%
! AGCGCAGATCTTGAGGGTTT+TGG + chr6_2:15475109-15475128 None:intergenic 50.0%
GACGATTAGCGCAGATCTTG+AGG + chr6_2:15475116-15475135 None:intergenic 50.0%
CATGCTAGGGATTCTGCTGA+TGG - chr6_2:15475295-15475314 Msa0910660:CDS 50.0%
GTGTTGGAATCCGAGAGAGT+AGG - chr6_2:15475590-15475609 Msa0910660:CDS 50.0%
CCGAGAGAGTAGGGTGTATT+TGG - chr6_2:15475600-15475619 Msa0910660:CDS 50.0%
GTTGCACATGCCATCTTGTC+CGG - chr6_2:15475639-15475658 Msa0910660:intron 50.0%
GCAGTTCTGTTCGAGGAAGT+TGG - chr6_2:15475774-15475793 Msa0910660:CDS 50.0%
GCAACCGAATCACCATCTCA+TGG + chr6_2:15475835-15475854 None:intergenic 50.0%
GCTGCTTCAACTGTGTTTGC+AGG - chr6_2:15475866-15475885 Msa0910660:five_prime_UTR 50.0%
CGAGGAAGTTGGGGTCTAGT+TGG - chr6_2:15475785-15475804 Msa0910660:exon 55.0%
CGAGGAAGTTGGGGTCTAGT+TGG - chr6_2:15475785-15475804 Msa0910660:exon 55.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 15474961 15475934 15474961 ID=Msa0910660;Name=Msa0910660
chr6_2 mRNA 15474961 15475934 15474961 ID=Msa0910660-mRNA-1;Parent=Msa0910660;Name=Msa0910660-mRNA-1;_AED=0.39;_eAED=0.39;_QI=135|0.33|0.25|0.75|1|0.75|4|0|122
chr6_2 exon 15474961 15475135 15474961 ID=Msa0910660-mRNA-1:exon:3431;Parent=Msa0910660-mRNA-1
chr6_2 exon 15475274 15475343 15475274 ID=Msa0910660-mRNA-1:exon:3430;Parent=Msa0910660-mRNA-1
chr6_2 exon 15475579 15475656 15475579 ID=Msa0910660-mRNA-1:exon:3429;Parent=Msa0910660-mRNA-1
chr6_2 exon 15475757 15475934 15475757 ID=Msa0910660-mRNA-1:exon:3428;Parent=Msa0910660-mRNA-1
chr6_2 five_prime_UTR 15475800 15475934 15475800 ID=Msa0910660-mRNA-1:five_prime_utr;Parent=Msa0910660-mRNA-1
chr6_2 CDS 15475757 15475799 15475757 ID=Msa0910660-mRNA-1:cds;Parent=Msa0910660-mRNA-1
chr6_2 CDS 15475579 15475656 15475579 ID=Msa0910660-mRNA-1:cds;Parent=Msa0910660-mRNA-1
chr6_2 CDS 15475274 15475343 15475274 ID=Msa0910660-mRNA-1:cds;Parent=Msa0910660-mRNA-1
chr6_2 CDS 15474961 15475135 15474961 ID=Msa0910660-mRNA-1:cds;Parent=Msa0910660-mRNA-1
Gene Sequence

>Msa0910660

ATGAGGCAATCCAAAACCCTCAAGATCTGCGCTAATCGTCTTATTATACCTATGATGAGTATGCAAGAGAATGTTGGAATTGAGAAATCGTGTGTTTGGCATGCTAGGGATTCTGCTGATGTGAATGAATTAATTGATGATAACTATCTTGTTAAAGTGGTGTTGGAATCCGAGAGAGTAGGGTGTATTTGGGTGTTTGTCTTTTGCAGTTCTGTTCGAGGAAGTTGGGGTCTAGTTGGCTATAGATCAATCAGTTTTTTCTGCCATGAGATGGTGATTCGGTTGCTAGCAGTTTGGGCTGCTTCAACTGTGTTTGCAGGGGTATATGTTGGTGTGCTTTCTGGTTTGATTTCTGCTCCAGTCCAT

Protein sequence

>Msa0910660

MRQSKTLKICANRLIIPMMSMQENVGIEKSCVWHARDSADVNELIDDNYLVKVVLESERVGCIWVFVFCSSVRGSWGLVGYRSISFFCHEMVIRLLAVWAASTVFAGVYVGVLSGLISAPVH