AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0940320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0940320 MtrunA17_Chr3g0144421 97.419 310 5 3 1 310 1 307 0.0 615
Msa0940320 MtrunA17_Chr3g0144451 54.911 224 100 1 83 305 52 275 2.32e-91 273
Msa0940320 MtrunA17_Chr4g0034821 54.464 224 101 1 83 305 68 291 5.56e-90 270
Msa0940320 MtrunA17_Chr3g0138231 51.852 243 111 3 69 305 24 266 6.78e-88 264
Msa0940320 MtrunA17_Chr4g0034451 45.852 229 118 4 80 305 301 526 5.31e-66 216
Msa0940320 MtrunA17_Chr4g0034731 43.636 220 118 4 90 306 217 433 4.89e-63 205
Msa0940320 MtrunA17_Chr3g0144411 42.466 219 122 2 89 306 324 539 6.59e-63 207
Msa0940320 MtrunA17_Chr3g0144391 41.096 219 125 2 89 306 291 506 6.73e-63 207
Msa0940320 MtrunA17_Chr4g0034721 44.091 220 117 4 90 306 361 577 2.39e-58 196
Msa0940320 MtrunA17_Chr1g0147271 43.243 222 120 4 85 304 166 383 2.77e-58 192
Msa0940320 MtrunA17_Chr4g0034691 43.636 220 118 4 90 306 493 709 3.48e-57 196
Msa0940320 MtrunA17_Chr1g0147321 41.704 223 121 5 87 307 24 239 8.96e-55 179
Msa0940320 MtrunA17_Chr3g0139431 42.478 226 121 6 84 305 101 321 4.46e-54 179
Msa0940320 MtrunA17_Chr1g0147301 41.629 221 122 5 87 304 67 283 7.45e-54 177
Msa0940320 MtrunA17_Chr1g0147291 41.818 220 119 5 87 304 53 265 1.48e-53 176
Msa0940320 MtrunA17_Chr1g0147331 42.601 223 118 7 85 304 108 323 6.57e-53 176
Msa0940320 MtrunA17_Chr1g0147311 42.105 228 123 6 82 304 131 354 2.06e-52 176
Msa0940320 MtrunA17_Chr1g0146631 42.152 223 119 7 85 304 108 323 2.78e-52 174
Msa0940320 MtrunA17_Chr1g0147261 42.202 218 119 5 87 303 86 297 5.23e-52 173
Msa0940320 MtrunA17_Chr1g0147251 40.826 218 119 5 87 303 32 240 1.52e-49 165
Msa0940320 MtrunA17_Chr1g0147241 39.474 228 128 5 77 303 92 310 2.37e-49 166
Msa0940320 MtrunA17_Chr6g0485051 40.000 195 113 4 115 305 11 205 3.89e-48 160
Msa0940320 MtrunA17_Chr5g0413591 31.984 247 157 4 59 304 391 627 1.83e-30 120
Msa0940320 MtrunA17_Chr8g0365361 31.797 217 142 4 90 304 181 393 9.67e-30 116
Msa0940320 MtrunA17_Chr8g0365381 32.407 216 140 4 90 303 429 640 9.93e-29 115
Msa0940320 MtrunA17_Chr8g0365401 33.641 217 138 5 90 304 412 624 7.98e-28 113
Msa0940320 MtrunA17_Chr3g0117791 32.258 217 141 4 90 304 413 625 6.64e-27 110
Msa0940320 MtrunA17_Chr5g0412991 31.193 218 144 5 88 303 195 408 3.24e-26 107
Msa0940320 MtrunA17_Chr5g0412991 30.939 181 120 4 109 287 7 184 8.80e-20 89.0
Msa0940320 MtrunA17_Chr5g0412981 31.193 218 144 5 88 303 195 408 3.24e-26 107
Msa0940320 MtrunA17_Chr5g0412981 30.939 181 120 4 109 287 7 184 1.23e-19 88.6
Msa0940320 MtrunA17_Chr5g0412951 30.457 197 132 4 109 303 14 207 4.34e-24 97.4
Msa0940320 MtrunA17_Chr4g0034051 47.500 80 40 2 229 306 1 80 7.57e-22 87.8
Msa0940320 MtrunA17_Chr4g0076231 33.333 156 100 3 150 303 3 156 3.26e-21 88.2
Msa0940320 MtrunA17_Chr8g0355861 37.143 140 79 5 166 305 130 260 4.70e-21 90.9
Msa0940320 MtrunA17_Chr8g0355331 32.948 173 100 6 133 305 265 421 7.65e-21 92.4
Msa0940320 MtrunA17_Chr8g0355311 35.714 140 81 5 166 305 60 190 2.39e-20 87.0
Msa0940320 MtrunA17_Chr8g0355841 39.286 140 76 5 166 305 379 509 1.52e-19 89.0
Msa0940320 MtrunA17_Chr8g0355321 35.000 140 82 5 166 305 172 302 4.84e-19 85.5
Msa0940320 MtrunA17_Chr2g0318901 33.333 141 85 5 165 305 128 259 1.68e-18 83.6
Msa0940320 MtrunA17_Chr8g0355871 34.416 154 91 6 154 306 79 223 3.49e-18 82.0
Msa0940320 MtrunA17_Chr8g0355831 38.571 140 77 5 166 305 221 351 3.57e-18 84.0
Msa0940320 MtrunA17_Chr1g0168781 32.653 147 91 5 159 305 121 259 4.86e-18 82.4
Msa0940320 MtrunA17_Chr2g0318871 28.440 218 123 9 90 305 73 259 3.67e-16 77.0
Msa0940320 MtrunA17_Chr8g0371951 38.824 85 49 2 212 294 4 87 2.64e-14 67.4
Msa0940320 MtrunA17_Chr5g0413021 29.032 124 84 3 181 303 7 127 5.21e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0940320 AT1G49320.1 48.899 227 110 3 84 304 52 278 8.41e-73 226
Msa0940320 AT5G25610.1 42.857 224 120 5 84 303 170 389 8.18e-56 186
Msa0940320 AT1G70370.2 33.333 222 132 6 90 304 411 623 3.92e-26 108
Msa0940320 AT1G70370.1 33.333 222 132 6 90 304 411 623 3.92e-26 108
Msa0940320 AT1G23760.1 32.883 222 133 7 90 304 407 619 9.00e-25 104
Msa0940320 AT1G60390.1 30.804 224 141 5 86 303 405 620 8.09e-24 102

Find 71 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCACATGGATACTTCTAAA+TGG 0.212561 6_3:-12582134 Msa0940320:CDS
ACATTGTTATCTGTACTATT+TGG 0.233418 6_3:-12583252 Msa0940320:five_prime_UTR
TTTCTTCCCTCCTGATAATC+TGG 0.262557 6_3:-12582049 Msa0940320:CDS
AAACTAGGGAGTTGATTAAT+TGG 0.264198 6_3:+12583148 None:intergenic
CCCTCCTGATAATCTGGTTT+GGG 0.285119 6_3:-12582043 Msa0940320:CDS
GACTTTGCAAATTACATGTT+TGG 0.295983 6_3:-12582393 Msa0940320:CDS
TCCCTCCTGATAATCTGGTT+TGG 0.305714 6_3:-12582044 Msa0940320:CDS
CCGTAAGGGAAAGGAATATA+AGG 0.308964 6_3:+12583110 None:intergenic
ATTAATTGGATGTAGGTCTA+AGG 0.311404 6_3:+12583162 None:intergenic
ATTTCAGTTCCAAATGCAAT+AGG 0.315492 6_3:-12582279 Msa0940320:CDS
TAGAAAGATAACTATAATTA+AGG 0.316435 6_3:-12582853 Msa0940320:CDS
AGCAGTTGATGAATCTTTCT+TGG 0.317340 6_3:+12582611 None:intergenic
GTTCCAAAGGATTTAAAAGT+TGG 0.342461 6_3:-12582657 Msa0940320:CDS
TTATAGTTGACATAGTATTA+TGG 0.347756 6_3:+12582003 None:intergenic
GGCACCCAAACCAGATTATC+AGG 0.351108 6_3:+12582039 None:intergenic
AAATTGTGTAATTCTGTAAA+GGG 0.354659 6_3:+12582316 None:intergenic
AAGTGTGTATTTCATGGCTT+TGG 0.373004 6_3:+12582485 None:intergenic
TTGTTCAAGTGTGTATTTCA+TGG 0.375445 6_3:+12582479 None:intergenic
TACTACAATTTCATACAAAC+TGG 0.390862 6_3:+12582197 None:intergenic
TTGTATGAAATTGTAGTAGA+AGG 0.391678 6_3:-12582192 Msa0940320:CDS
ATTGTAGTAGAAGGTGAAAA+TGG 0.391681 6_3:-12582183 Msa0940320:CDS
CCTTATATTCCTTTCCCTTA+CGG 0.393008 6_3:-12583110 Msa0940320:five_prime_UTR
TGCTATTCACATGGGAAACT+TGG 0.395953 6_3:+12583188 None:intergenic
TGACATACTGGAGAGTTTCC+AGG 0.407905 6_3:+12582072 None:intergenic
AAAAGACTAGTAGGGTATAT+AGG 0.408467 6_3:+12583000 None:intergenic
GAAATTGTGTAATTCTGTAA+AGG 0.412679 6_3:+12582315 None:intergenic
AAAGTCAAACAAAGACTCTA+AGG 0.413155 6_3:+12582410 None:intergenic
TGACAATAGAAAACTGCAAA+AGG 0.418592 6_3:+12582237 None:intergenic
ACAAACTGGTATCACCCTTC+TGG 0.421146 6_3:+12582211 None:intergenic
GTGGAGTGTGTAGGAAAACT+AGG 0.427800 6_3:+12583133 None:intergenic
GGGTGACACCCTATTGCATT+TGG 0.430364 6_3:+12582270 None:intergenic
AGTTGGTGTATTTGATTTGA+AGG 0.431479 6_3:+12583297 None:intergenic
TTGTTATCTGTACTATTTGG+TGG 0.433114 6_3:-12583249 Msa0940320:five_prime_UTR
AGATGGAGAAGAAATCTAGA+AGG 0.448342 6_3:+12582526 None:intergenic
TCACATGGATACTTCTAAAT+GGG 0.449148 6_3:-12582133 Msa0940320:CDS
TTTCAGTTCCAAATGCAATA+GGG 0.450082 6_3:-12582278 Msa0940320:CDS
TATTTCATGGCTTTGGCTTG+TGG 0.464005 6_3:+12582492 None:intergenic
GTAATTCTGTAAAGGGATAG+AGG 0.467198 6_3:+12582323 None:intergenic
GAGAAGAAATCTAGAAGGAA+AGG 0.485932 6_3:+12582531 None:intergenic
GGAGTTGATTAATTGGATGT+AGG 0.488259 6_3:+12583155 None:intergenic
CGTGTGCTTAATGTAAAACC+TGG 0.488283 6_3:-12582090 Msa0940320:CDS
GGAGGGAAGAAATGACATAC+TGG 0.503691 6_3:+12582060 None:intergenic
TTAATTGGATGTAGGTCTAA+GGG 0.509791 6_3:+12583163 None:intergenic
CTGCAAAAGGGTAAGGCATA+GGG 0.513892 6_3:+12582250 None:intergenic
TGGAGTGTGTAGGAAAACTA+GGG 0.514055 6_3:+12583134 None:intergenic
TGTAAGCTGTTGCAAATCAT+AGG 0.524928 6_3:+12582824 None:intergenic
TCTAGTAAATAATAGTATCA+TGG 0.525126 6_3:-12582790 Msa0940320:CDS
TAGAAAACTGCAAAAGGGTA+AGG 0.531191 6_3:+12582243 None:intergenic
TTGTAGGTTCCGTAAGGGAA+AGG 0.532944 6_3:+12583101 None:intergenic
GACAATAGAAAACTGCAAAA+GGG 0.537400 6_3:+12582238 None:intergenic
TATTATGGTGTTGCAGTCAA+AGG 0.553743 6_3:+12582018 None:intergenic
CTAAGGGGTTGCTATTCACA+TGG 0.558604 6_3:+12583179 None:intergenic
AATATAAGGGTGGAGTGTGT+AGG 0.560507 6_3:+12583124 None:intergenic
ACTGCAAAAGGGTAAGGCAT+AGG 0.564319 6_3:+12582249 None:intergenic
AGCTGCAGCAATTTGTCACA+TGG 0.567177 6_3:-12582148 Msa0940320:CDS
TTTCTATTGTCACAGCCAGA+AGG 0.572783 6_3:-12582226 Msa0940320:CDS
CGTAAGGGAAAGGAATATAA+GGG 0.583432 6_3:+12583111 None:intergenic
CAACAGCTTACAACAGAAGA+GGG 0.584548 6_3:-12582813 Msa0940320:CDS
ATATTAGTAGTAGTGCAGTC+AGG 0.585822 6_3:+12583225 None:intergenic
GCAACAGCTTACAACAGAAG+AGG 0.592350 6_3:-12582814 Msa0940320:CDS
TTCTATTGTCACAGCCAGAA+GGG 0.596892 6_3:-12582225 Msa0940320:CDS
TTTGTTTGTAGGTTCCGTAA+GGG 0.602691 6_3:+12583096 None:intergenic
TTTATAATATGGGAGGAGGG+AGG 0.603327 6_3:+12581976 None:intergenic
GCACTACTACTAATATAATG+AGG 0.607836 6_3:-12583218 Msa0940320:five_prime_UTR
TAATTGGATGTAGGTCTAAG+GGG 0.616446 6_3:+12583164 None:intergenic
ATAGTACAGATAACAATGTG+TGG 0.618170 6_3:+12583256 None:intergenic
GTAGTAGAAGGTGAAAATGG+AGG 0.623165 6_3:-12582180 Msa0940320:CDS
CCCAAACCAGATTATCAGGA+GGG 0.634612 6_3:+12582043 None:intergenic
ACCCAAACCAGATTATCAGG+AGG 0.664591 6_3:+12582042 None:intergenic
TAAGGGGTTGCTATTCACAT+GGG 0.675323 6_3:+12583180 None:intergenic
AAGGGAAAGGAATATAAGGG+TGG 0.701614 6_3:+12583114 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAATATAAACATTAGT+TGG + chr6_3:12582013-12582032 None:intergenic 10.0%
!!! ATTTTAATATAAACATTAGT+TGG + chr6_3:12582013-12582032 None:intergenic 10.0%
!!! TAATTTTTCTCTTTTATGAT+TGG - chr6_3:12582386-12582405 Msa0940320:CDS 15.0%
!! TAGAAAGATAACTATAATTA+AGG - chr6_3:12582437-12582456 Msa0940320:CDS 15.0%
!!! TAATTTTTCTCTTTTATGAT+TGG - chr6_3:12582386-12582405 Msa0940320:CDS 15.0%
!! TAGAAAGATAACTATAATTA+AGG - chr6_3:12582437-12582456 Msa0940320:CDS 15.0%
!! TCTAGTAAATAATAGTATCA+TGG - chr6_3:12582500-12582519 Msa0940320:CDS 20.0%
!! TCTTTCTTGGAAAAATAAAT+TGG + chr6_3:12582669-12582688 None:intergenic 20.0%
!!! AAAACTTTGAATTTTGAGTT+GGG + chr6_3:12582924-12582943 None:intergenic 20.0%
!! AAATTGTGTAATTCTGTAAA+GGG + chr6_3:12582977-12582996 None:intergenic 20.0%
!! TTATAGTTGACATAGTATTA+TGG + chr6_3:12583290-12583309 None:intergenic 20.0%
!!! TAGTTTTTTTATAATATGGG+AGG + chr6_3:12583324-12583343 None:intergenic 20.0%
!! TCTAGTAAATAATAGTATCA+TGG - chr6_3:12582500-12582519 Msa0940320:CDS 20.0%
!! TCTTTCTTGGAAAAATAAAT+TGG + chr6_3:12582669-12582688 None:intergenic 20.0%
!!! AAAACTTTGAATTTTGAGTT+GGG + chr6_3:12582924-12582943 None:intergenic 20.0%
!! AAATTGTGTAATTCTGTAAA+GGG + chr6_3:12582977-12582996 None:intergenic 20.0%
!! TTATAGTTGACATAGTATTA+TGG + chr6_3:12583290-12583309 None:intergenic 20.0%
!!! TAGTTTTTTTATAATATGGG+AGG + chr6_3:12583324-12583343 None:intergenic 20.0%
! ACATTGTTATCTGTACTATT+TGG - chr6_3:12582038-12582057 Msa0940320:CDS 25.0%
!!! ATTTTTGGTGTTTTGTTTGT+AGG + chr6_3:12582208-12582227 None:intergenic 25.0%
! CAAACAAAACACCAAAAATA+AGG - chr6_3:12582209-12582228 Msa0940320:CDS 25.0%
! ACAGAAAAAGATGATTTCAA+AGG - chr6_3:12582566-12582585 Msa0940320:CDS 25.0%
!! TTTCCAACTTTTAAATCCTT+TGG + chr6_3:12582639-12582658 None:intergenic 25.0%
!!! ATTTTGTTAGCTTCTTCTTT+TGG + chr6_3:12582717-12582736 None:intergenic 25.0%
!!! CAAAACTTTGAATTTTGAGT+TGG + chr6_3:12582925-12582944 None:intergenic 25.0%
! GAAATTGTGTAATTCTGTAA+AGG + chr6_3:12582978-12582997 None:intergenic 25.0%
! TACTACAATTTCATACAAAC+TGG + chr6_3:12583096-12583115 None:intergenic 25.0%
! TTGTATGAAATTGTAGTAGA+AGG - chr6_3:12583098-12583117 Msa0940320:five_prime_UTR 25.0%
!!! TTTTTTATAATATGGGAGGA+GGG + chr6_3:12583320-12583339 None:intergenic 25.0%
!!! TTTTTTTATAATATGGGAGG+AGG + chr6_3:12583321-12583340 None:intergenic 25.0%
! ACATTGTTATCTGTACTATT+TGG - chr6_3:12582038-12582057 Msa0940320:CDS 25.0%
!!! ATTTTTGGTGTTTTGTTTGT+AGG + chr6_3:12582208-12582227 None:intergenic 25.0%
! CAAACAAAACACCAAAAATA+AGG - chr6_3:12582209-12582228 Msa0940320:CDS 25.0%
! ACAGAAAAAGATGATTTCAA+AGG - chr6_3:12582566-12582585 Msa0940320:CDS 25.0%
!! TTTCCAACTTTTAAATCCTT+TGG + chr6_3:12582639-12582658 None:intergenic 25.0%
!!! ATTTTGTTAGCTTCTTCTTT+TGG + chr6_3:12582717-12582736 None:intergenic 25.0%
!!! CAAAACTTTGAATTTTGAGT+TGG + chr6_3:12582925-12582944 None:intergenic 25.0%
! GAAATTGTGTAATTCTGTAA+AGG + chr6_3:12582978-12582997 None:intergenic 25.0%
! TACTACAATTTCATACAAAC+TGG + chr6_3:12583096-12583115 None:intergenic 25.0%
! TTGTATGAAATTGTAGTAGA+AGG - chr6_3:12583098-12583117 Msa0940320:five_prime_UTR 25.0%
!!! TTTTTTATAATATGGGAGGA+GGG + chr6_3:12583320-12583339 None:intergenic 25.0%
!!! TTTTTTTATAATATGGGAGG+AGG + chr6_3:12583321-12583340 None:intergenic 25.0%
!! AGTTGGTGTATTTGATTTGA+AGG + chr6_3:12581996-12582015 None:intergenic 30.0%
ATAGTACAGATAACAATGTG+TGG + chr6_3:12582037-12582056 None:intergenic 30.0%
TTGTTATCTGTACTATTTGG+TGG - chr6_3:12582041-12582060 Msa0940320:CDS 30.0%
GCACTACTACTAATATAATG+AGG - chr6_3:12582072-12582091 Msa0940320:CDS 30.0%
TTAATTGGATGTAGGTCTAA+GGG + chr6_3:12582130-12582149 None:intergenic 30.0%
ATTAATTGGATGTAGGTCTA+AGG + chr6_3:12582131-12582150 None:intergenic 30.0%
! AAACTAGGGAGTTGATTAAT+TGG + chr6_3:12582145-12582164 None:intergenic 30.0%
!!! GAAACTTAGGACCTTATTTT+TGG + chr6_3:12582223-12582242 None:intergenic 30.0%
!!! CTCTGTTTCTTTTGAAACTT+AGG + chr6_3:12582236-12582255 None:intergenic 30.0%
! TGAGACTTTTAATCTCTTCT+TGG - chr6_3:12582259-12582278 Msa0940320:CDS 30.0%
AAAAGACTAGTAGGGTATAT+AGG + chr6_3:12582293-12582312 None:intergenic 30.0%
!!! ATATACCCTACTAGTCTTTT+TGG - chr6_3:12582293-12582312 Msa0940320:CDS 30.0%
TCTTACCAAAAAGACTAGTA+GGG + chr6_3:12582301-12582320 None:intergenic 30.0%
ATCTTACCAAAAAGACTAGT+AGG + chr6_3:12582302-12582321 None:intergenic 30.0%
GTTCCAAAGGATTTAAAAGT+TGG - chr6_3:12582633-12582652 Msa0940320:CDS 30.0%
! TCTTCTTTTGGTAGGAATTT+TGG + chr6_3:12582705-12582724 None:intergenic 30.0%
!!! AGAAGGAAAGGTAGTTTTTT+TGG + chr6_3:12582750-12582769 None:intergenic 30.0%
TTGTTCAAGTGTGTATTTCA+TGG + chr6_3:12582814-12582833 None:intergenic 30.0%
! ACAATGCAATTTTGAACCTA+TGG - chr6_3:12582830-12582849 Msa0940320:CDS 30.0%
AAAGTCAAACAAAGACTCTA+AGG + chr6_3:12582883-12582902 None:intergenic 30.0%
GACTTTGCAAATTACATGTT+TGG - chr6_3:12582897-12582916 Msa0940320:intron 30.0%
ATTTCAGTTCCAAATGCAAT+AGG - chr6_3:12583011-12583030 Msa0940320:CDS 30.0%
TTTCAGTTCCAAATGCAATA+GGG - chr6_3:12583012-12583031 Msa0940320:CDS 30.0%
GACAATAGAAAACTGCAAAA+GGG + chr6_3:12583055-12583074 None:intergenic 30.0%
TGACAATAGAAAACTGCAAA+AGG + chr6_3:12583056-12583075 None:intergenic 30.0%
ATTGTAGTAGAAGGTGAAAA+TGG - chr6_3:12583107-12583126 Msa0940320:five_prime_UTR 30.0%
TCACATGGATACTTCTAAAT+GGG - chr6_3:12583157-12583176 Msa0940320:five_prime_UTR 30.0%
! TTTTACATTAAGCACACGAA+AGG + chr6_3:12583198-12583217 None:intergenic 30.0%
!! AGTTGGTGTATTTGATTTGA+AGG + chr6_3:12581996-12582015 None:intergenic 30.0%
ATAGTACAGATAACAATGTG+TGG + chr6_3:12582037-12582056 None:intergenic 30.0%
TTGTTATCTGTACTATTTGG+TGG - chr6_3:12582041-12582060 Msa0940320:CDS 30.0%
GCACTACTACTAATATAATG+AGG - chr6_3:12582072-12582091 Msa0940320:CDS 30.0%
TTAATTGGATGTAGGTCTAA+GGG + chr6_3:12582130-12582149 None:intergenic 30.0%
ATTAATTGGATGTAGGTCTA+AGG + chr6_3:12582131-12582150 None:intergenic 30.0%
! AAACTAGGGAGTTGATTAAT+TGG + chr6_3:12582145-12582164 None:intergenic 30.0%
!!! GAAACTTAGGACCTTATTTT+TGG + chr6_3:12582223-12582242 None:intergenic 30.0%
!!! CTCTGTTTCTTTTGAAACTT+AGG + chr6_3:12582236-12582255 None:intergenic 30.0%
! TGAGACTTTTAATCTCTTCT+TGG - chr6_3:12582259-12582278 Msa0940320:CDS 30.0%
AAAAGACTAGTAGGGTATAT+AGG + chr6_3:12582293-12582312 None:intergenic 30.0%
!!! ATATACCCTACTAGTCTTTT+TGG - chr6_3:12582293-12582312 Msa0940320:CDS 30.0%
TCTTACCAAAAAGACTAGTA+GGG + chr6_3:12582301-12582320 None:intergenic 30.0%
ATCTTACCAAAAAGACTAGT+AGG + chr6_3:12582302-12582321 None:intergenic 30.0%
GTTCCAAAGGATTTAAAAGT+TGG - chr6_3:12582633-12582652 Msa0940320:CDS 30.0%
! TCTTCTTTTGGTAGGAATTT+TGG + chr6_3:12582705-12582724 None:intergenic 30.0%
!!! AGAAGGAAAGGTAGTTTTTT+TGG + chr6_3:12582750-12582769 None:intergenic 30.0%
TTGTTCAAGTGTGTATTTCA+TGG + chr6_3:12582814-12582833 None:intergenic 30.0%
! ACAATGCAATTTTGAACCTA+TGG - chr6_3:12582830-12582849 Msa0940320:CDS 30.0%
AAAGTCAAACAAAGACTCTA+AGG + chr6_3:12582883-12582902 None:intergenic 30.0%
GACTTTGCAAATTACATGTT+TGG - chr6_3:12582897-12582916 Msa0940320:intron 30.0%
ATTTCAGTTCCAAATGCAAT+AGG - chr6_3:12583011-12583030 Msa0940320:CDS 30.0%
TTTCAGTTCCAAATGCAATA+GGG - chr6_3:12583012-12583031 Msa0940320:CDS 30.0%
GACAATAGAAAACTGCAAAA+GGG + chr6_3:12583055-12583074 None:intergenic 30.0%
TGACAATAGAAAACTGCAAA+AGG + chr6_3:12583056-12583075 None:intergenic 30.0%
ATTGTAGTAGAAGGTGAAAA+TGG - chr6_3:12583107-12583126 Msa0940320:five_prime_UTR 30.0%
TCACATGGATACTTCTAAAT+GGG - chr6_3:12583157-12583176 Msa0940320:five_prime_UTR 30.0%
! TTTTACATTAAGCACACGAA+AGG + chr6_3:12583198-12583217 None:intergenic 30.0%
ATATTAGTAGTAGTGCAGTC+AGG + chr6_3:12582068-12582087 None:intergenic 35.0%
TAATTGGATGTAGGTCTAAG+GGG + chr6_3:12582129-12582148 None:intergenic 35.0%
! GGAGTTGATTAATTGGATGT+AGG + chr6_3:12582138-12582157 None:intergenic 35.0%
CGTAAGGGAAAGGAATATAA+GGG + chr6_3:12582182-12582201 None:intergenic 35.0%
CCTTATATTCCTTTCCCTTA+CGG - chr6_3:12582180-12582199 Msa0940320:CDS 35.0%
TTTGTTTGTAGGTTCCGTAA+GGG + chr6_3:12582197-12582216 None:intergenic 35.0%
!! TTTTGTTTGTAGGTTCCGTA+AGG + chr6_3:12582198-12582217 None:intergenic 35.0%
TGTAAGCTGTTGCAAATCAT+AGG + chr6_3:12582469-12582488 None:intergenic 35.0%
CGAAAAACACGTTCAATTCA+GGG + chr6_3:12582614-12582633 None:intergenic 35.0%
ACGAAAAACACGTTCAATTC+AGG + chr6_3:12582615-12582634 None:intergenic 35.0%
!! AGCAGTTGATGAATCTTTCT+TGG + chr6_3:12582682-12582701 None:intergenic 35.0%
!! TGTTAGCTTCTTCTTTTGGT+AGG + chr6_3:12582713-12582732 None:intergenic 35.0%
!!! GGTAGTTTTTTTGGTGTGAA+AGG + chr6_3:12582741-12582760 None:intergenic 35.0%
GAGAAGAAATCTAGAAGGAA+AGG + chr6_3:12582762-12582781 None:intergenic 35.0%
AGATGGAGAAGAAATCTAGA+AGG + chr6_3:12582767-12582786 None:intergenic 35.0%
!!! TTGTGGAGAGTTTTTTGAGA+TGG + chr6_3:12582784-12582803 None:intergenic 35.0%
AAGTGTGTATTTCATGGCTT+TGG + chr6_3:12582808-12582827 None:intergenic 35.0%
! TGCAATTTTGAACCTATGGA+AGG - chr6_3:12582834-12582853 Msa0940320:CDS 35.0%
! AATTTTGTCTCTCCTTCCAT+AGG + chr6_3:12582849-12582868 None:intergenic 35.0%
! GATAGAGGATTTTGTGACAT+GGG + chr6_3:12582955-12582974 None:intergenic 35.0%
GTAATTCTGTAAAGGGATAG+AGG + chr6_3:12582970-12582989 None:intergenic 35.0%
TAGAAAACTGCAAAAGGGTA+AGG + chr6_3:12583050-12583069 None:intergenic 35.0%
GTCACATGGATACTTCTAAA+TGG - chr6_3:12583156-12583175 Msa0940320:five_prime_UTR 35.0%
!! TATTATGGTGTTGCAGTCAA+AGG + chr6_3:12583275-12583294 None:intergenic 35.0%
ATATTAGTAGTAGTGCAGTC+AGG + chr6_3:12582068-12582087 None:intergenic 35.0%
TAATTGGATGTAGGTCTAAG+GGG + chr6_3:12582129-12582148 None:intergenic 35.0%
! GGAGTTGATTAATTGGATGT+AGG + chr6_3:12582138-12582157 None:intergenic 35.0%
CGTAAGGGAAAGGAATATAA+GGG + chr6_3:12582182-12582201 None:intergenic 35.0%
CCTTATATTCCTTTCCCTTA+CGG - chr6_3:12582180-12582199 Msa0940320:CDS 35.0%
TTTGTTTGTAGGTTCCGTAA+GGG + chr6_3:12582197-12582216 None:intergenic 35.0%
!! TTTTGTTTGTAGGTTCCGTA+AGG + chr6_3:12582198-12582217 None:intergenic 35.0%
TGTAAGCTGTTGCAAATCAT+AGG + chr6_3:12582469-12582488 None:intergenic 35.0%
CGAAAAACACGTTCAATTCA+GGG + chr6_3:12582614-12582633 None:intergenic 35.0%
ACGAAAAACACGTTCAATTC+AGG + chr6_3:12582615-12582634 None:intergenic 35.0%
!! AGCAGTTGATGAATCTTTCT+TGG + chr6_3:12582682-12582701 None:intergenic 35.0%
!! TGTTAGCTTCTTCTTTTGGT+AGG + chr6_3:12582713-12582732 None:intergenic 35.0%
!!! GGTAGTTTTTTTGGTGTGAA+AGG + chr6_3:12582741-12582760 None:intergenic 35.0%
GAGAAGAAATCTAGAAGGAA+AGG + chr6_3:12582762-12582781 None:intergenic 35.0%
AGATGGAGAAGAAATCTAGA+AGG + chr6_3:12582767-12582786 None:intergenic 35.0%
!!! TTGTGGAGAGTTTTTTGAGA+TGG + chr6_3:12582784-12582803 None:intergenic 35.0%
AAGTGTGTATTTCATGGCTT+TGG + chr6_3:12582808-12582827 None:intergenic 35.0%
! TGCAATTTTGAACCTATGGA+AGG - chr6_3:12582834-12582853 Msa0940320:CDS 35.0%
! AATTTTGTCTCTCCTTCCAT+AGG + chr6_3:12582849-12582868 None:intergenic 35.0%
! GATAGAGGATTTTGTGACAT+GGG + chr6_3:12582955-12582974 None:intergenic 35.0%
GTAATTCTGTAAAGGGATAG+AGG + chr6_3:12582970-12582989 None:intergenic 35.0%
TAGAAAACTGCAAAAGGGTA+AGG + chr6_3:12583050-12583069 None:intergenic 35.0%
GTCACATGGATACTTCTAAA+TGG - chr6_3:12583156-12583175 Msa0940320:five_prime_UTR 35.0%
!! TATTATGGTGTTGCAGTCAA+AGG + chr6_3:12583275-12583294 None:intergenic 35.0%
TGCTATTCACATGGGAAACT+TGG + chr6_3:12582105-12582124 None:intergenic 40.0%
! TAAGGGGTTGCTATTCACAT+GGG + chr6_3:12582113-12582132 None:intergenic 40.0%
TGGAGTGTGTAGGAAAACTA+GGG + chr6_3:12582159-12582178 None:intergenic 40.0%
AATATAAGGGTGGAGTGTGT+AGG + chr6_3:12582169-12582188 None:intergenic 40.0%
AAGGGAAAGGAATATAAGGG+TGG + chr6_3:12582179-12582198 None:intergenic 40.0%
CCGTAAGGGAAAGGAATATA+AGG + chr6_3:12582183-12582202 None:intergenic 40.0%
CAACAGCTTACAACAGAAGA+GGG - chr6_3:12582477-12582496 Msa0940320:CDS 40.0%
! GAACGTGTTTTTCGTTCCAA+AGG - chr6_3:12582620-12582639 Msa0940320:CDS 40.0%
!! TATTTCATGGCTTTGGCTTG+TGG + chr6_3:12582801-12582820 None:intergenic 40.0%
! GGATAGAGGATTTTGTGACA+TGG + chr6_3:12582956-12582975 None:intergenic 40.0%
TTTCTATTGTCACAGCCAGA+AGG - chr6_3:12583064-12583083 Msa0940320:five_prime_UTR 40.0%
TTCTATTGTCACAGCCAGAA+GGG - chr6_3:12583065-12583084 Msa0940320:five_prime_UTR 40.0%
GTAGTAGAAGGTGAAAATGG+AGG - chr6_3:12583110-12583129 Msa0940320:five_prime_UTR 40.0%
CGTGTGCTTAATGTAAAACC+TGG - chr6_3:12583200-12583219 Msa0940320:five_prime_UTR 40.0%
TTTCTTCCCTCCTGATAATC+TGG - chr6_3:12583241-12583260 Msa0940320:five_prime_UTR 40.0%
TTTATAATATGGGAGGAGGG+AGG + chr6_3:12583317-12583336 None:intergenic 40.0%
TGCTATTCACATGGGAAACT+TGG + chr6_3:12582105-12582124 None:intergenic 40.0%
! TAAGGGGTTGCTATTCACAT+GGG + chr6_3:12582113-12582132 None:intergenic 40.0%
TGGAGTGTGTAGGAAAACTA+GGG + chr6_3:12582159-12582178 None:intergenic 40.0%
AATATAAGGGTGGAGTGTGT+AGG + chr6_3:12582169-12582188 None:intergenic 40.0%
AAGGGAAAGGAATATAAGGG+TGG + chr6_3:12582179-12582198 None:intergenic 40.0%
CCGTAAGGGAAAGGAATATA+AGG + chr6_3:12582183-12582202 None:intergenic 40.0%
CAACAGCTTACAACAGAAGA+GGG - chr6_3:12582477-12582496 Msa0940320:CDS 40.0%
! GAACGTGTTTTTCGTTCCAA+AGG - chr6_3:12582620-12582639 Msa0940320:CDS 40.0%
!! TATTTCATGGCTTTGGCTTG+TGG + chr6_3:12582801-12582820 None:intergenic 40.0%
! GGATAGAGGATTTTGTGACA+TGG + chr6_3:12582956-12582975 None:intergenic 40.0%
TTTCTATTGTCACAGCCAGA+AGG - chr6_3:12583064-12583083 Msa0940320:five_prime_UTR 40.0%
TTCTATTGTCACAGCCAGAA+GGG - chr6_3:12583065-12583084 Msa0940320:five_prime_UTR 40.0%
GTAGTAGAAGGTGAAAATGG+AGG - chr6_3:12583110-12583129 Msa0940320:five_prime_UTR 40.0%
CGTGTGCTTAATGTAAAACC+TGG - chr6_3:12583200-12583219 Msa0940320:five_prime_UTR 40.0%
TTTCTTCCCTCCTGATAATC+TGG - chr6_3:12583241-12583260 Msa0940320:five_prime_UTR 40.0%
TTTATAATATGGGAGGAGGG+AGG + chr6_3:12583317-12583336 None:intergenic 40.0%
! CTAAGGGGTTGCTATTCACA+TGG + chr6_3:12582114-12582133 None:intergenic 45.0%
GTGGAGTGTGTAGGAAAACT+AGG + chr6_3:12582160-12582179 None:intergenic 45.0%
TTGTAGGTTCCGTAAGGGAA+AGG + chr6_3:12582192-12582211 None:intergenic 45.0%
GCAACAGCTTACAACAGAAG+AGG - chr6_3:12582476-12582495 Msa0940320:CDS 45.0%
!! GGATTTTGTGACATGGGTTG+TGG + chr6_3:12582949-12582968 None:intergenic 45.0%
CTGCAAAAGGGTAAGGCATA+GGG + chr6_3:12583043-12583062 None:intergenic 45.0%
ACTGCAAAAGGGTAAGGCAT+AGG + chr6_3:12583044-12583063 None:intergenic 45.0%
ACAAACTGGTATCACCCTTC+TGG + chr6_3:12583082-12583101 None:intergenic 45.0%
AGCTGCAGCAATTTGTCACA+TGG - chr6_3:12583142-12583161 Msa0940320:five_prime_UTR 45.0%
TGACATACTGGAGAGTTTCC+AGG + chr6_3:12583221-12583240 None:intergenic 45.0%
GGAGGGAAGAAATGACATAC+TGG + chr6_3:12583233-12583252 None:intergenic 45.0%
TCCCTCCTGATAATCTGGTT+TGG - chr6_3:12583246-12583265 Msa0940320:five_prime_UTR 45.0%
CCCAAACCAGATTATCAGGA+GGG + chr6_3:12583250-12583269 None:intergenic 45.0%
! CCCTCCTGATAATCTGGTTT+GGG - chr6_3:12583247-12583266 Msa0940320:five_prime_UTR 45.0%
ACCCAAACCAGATTATCAGG+AGG + chr6_3:12583251-12583270 None:intergenic 45.0%
! CTAAGGGGTTGCTATTCACA+TGG + chr6_3:12582114-12582133 None:intergenic 45.0%
GTGGAGTGTGTAGGAAAACT+AGG + chr6_3:12582160-12582179 None:intergenic 45.0%
TTGTAGGTTCCGTAAGGGAA+AGG + chr6_3:12582192-12582211 None:intergenic 45.0%
GCAACAGCTTACAACAGAAG+AGG - chr6_3:12582476-12582495 Msa0940320:CDS 45.0%
!! GGATTTTGTGACATGGGTTG+TGG + chr6_3:12582949-12582968 None:intergenic 45.0%
CTGCAAAAGGGTAAGGCATA+GGG + chr6_3:12583043-12583062 None:intergenic 45.0%
ACTGCAAAAGGGTAAGGCAT+AGG + chr6_3:12583044-12583063 None:intergenic 45.0%
ACAAACTGGTATCACCCTTC+TGG + chr6_3:12583082-12583101 None:intergenic 45.0%
AGCTGCAGCAATTTGTCACA+TGG - chr6_3:12583142-12583161 Msa0940320:five_prime_UTR 45.0%
TGACATACTGGAGAGTTTCC+AGG + chr6_3:12583221-12583240 None:intergenic 45.0%
GGAGGGAAGAAATGACATAC+TGG + chr6_3:12583233-12583252 None:intergenic 45.0%
TCCCTCCTGATAATCTGGTT+TGG - chr6_3:12583246-12583265 Msa0940320:five_prime_UTR 45.0%
CCCAAACCAGATTATCAGGA+GGG + chr6_3:12583250-12583269 None:intergenic 45.0%
! CCCTCCTGATAATCTGGTTT+GGG - chr6_3:12583247-12583266 Msa0940320:five_prime_UTR 45.0%
ACCCAAACCAGATTATCAGG+AGG + chr6_3:12583251-12583270 None:intergenic 45.0%
!!! TTTTAGTTTTTTTATAATAT+GGG + chr6_3:12583327-12583346 None:intergenic 5.0%
!!! TTTTTAGTTTTTTTATAATA+TGG + chr6_3:12583328-12583347 None:intergenic 5.0%
!!! TTTTAGTTTTTTTATAATAT+GGG + chr6_3:12583327-12583346 None:intergenic 5.0%
!!! TTTTTAGTTTTTTTATAATA+TGG + chr6_3:12583328-12583347 None:intergenic 5.0%
GGGTGACACCCTATTGCATT+TGG + chr6_3:12583023-12583042 None:intergenic 50.0%
GGCACCCAAACCAGATTATC+AGG + chr6_3:12583254-12583273 None:intergenic 50.0%
GGGTGACACCCTATTGCATT+TGG + chr6_3:12583023-12583042 None:intergenic 50.0%
GGCACCCAAACCAGATTATC+AGG + chr6_3:12583254-12583273 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 12581956 12583356 12581956 ID=Msa0940320;Name=Msa0940320
chr6_3 mRNA 12581956 12583356 12581956 ID=Msa0940320-mRNA-1;Parent=Msa0940320;Name=Msa0940320-mRNA-1;_AED=0.04;_eAED=0.04;_QI=302|1|1|1|1|1|2|64|310
chr6_3 exon 12581956 12582895 12581956 ID=Msa0940320-mRNA-1:exon:4135;Parent=Msa0940320-mRNA-1
chr6_3 exon 12582998 12583356 12582998 ID=Msa0940320-mRNA-1:exon:4134;Parent=Msa0940320-mRNA-1
chr6_3 five_prime_UTR 12583055 12583356 12583055 ID=Msa0940320-mRNA-1:five_prime_utr;Parent=Msa0940320-mRNA-1
chr6_3 CDS 12582998 12583054 12582998 ID=Msa0940320-mRNA-1:cds;Parent=Msa0940320-mRNA-1
chr6_3 CDS 12582020 12582895 12582020 ID=Msa0940320-mRNA-1:cds;Parent=Msa0940320-mRNA-1
chr6_3 three_prime_UTR 12581956 12582019 12581956 ID=Msa0940320-mRNA-1:three_prime_utr;Parent=Msa0940320-mRNA-1
Gene Sequence

>Msa0940320

ATGAGACTTTTAATCTCTTCTTGGAGCTTCATCCTATATACCCTACTAGTCTTTTTGATTGTACAAGAGAGTTGTGCTAGAAAGATAACTATAATTAAGGATGCAGCCTATGATTTGCAACAGCTTACAACAGAAGAGGGTCTAGTAAATAATAGTATCATGGAAGTTGAAAACTCTGATCATGTAGAAATGAATAAAAAAGATGAACAGAAAAAGATGATTTCAAAGGATGTGCATCACAATCAAATAGACCCTGAATTGAACGTGTTTTTCGTTCCAAAGGATTTAAAAGTTGGAAAAATAATGCCAATTTATTTTTCCAAGAAAGATTCATCAACTGCTCCAAAATTCCTACCAAAAGAAGAAGCTAACAAAATTCCTTTCACACCAAAAAAACTACCTTTCCTTCTAGATTTCTTCTCCATCTCAAAAAACTCTCCACAAGCCAAAGCCATGAAATACACACTTGAACAATGCAATTTTGAACCTATGGAAGGAGAGACAAAATTCTGTGCTACATCCTTAGAGTCTTTGTTTGACTTTGCAAATTACATGTTTGGACCCAACTCAAAATTCAAAGTTTTGACCACAACCCATGTCACAAAATCCTCTATCCCTTTACAGAATTACACAATTTCAAAAGTAAATGAAATTTCAGTTCCAAATGCAATAGGGTGTCACCCTATGCCTTACCCTTTTGCAGTTTTCTATTGTCACAGCCAGAAGGGTGATACCAGTTTGTATGAAATTGTAGTAGAAGGTGAAAATGGAGGAATTGTTCAAGCTGCAGCAATTTGTCACATGGATACTTCTAAATGGGATGCAGATCATGTTGCCTTTCGTGTGCTTAATGTAAAACCTGGAAACTCTCCAGTATGTCATTTCTTCCCTCCTGATAATCTGGTTTGGGTGCCTTTGACTGCAACACCATAA
Protein sequence

>Msa0940320

MRLLISSWSFILYTLLVFLIVQESCARKITIIKDAAYDLQQLTTEEGLVNNSIMEVENSDHVEMNKKDEQKKMISKDVHHNQIDPELNVFFVPKDLKVGKIMPIYFSKKDSSTAPKFLPKEEANKIPFTPKKLPFLLDFFSISKNSPQAKAMKYTLEQCNFEPMEGETKFCATSLESLFDFANYMFGPNSKFKVLTTTHVTKSSIPLQNYTISKVNEISVPNAIGCHPMPYPFAVFYCHSQKGDTSLYEIVVEGENGGIVQAAAICHMDTSKWDADHVAFRVLNVKPGNSPVCHFFPPDNLVWVPLTATP*