AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0940130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0940130 MtrunA17_Chr3g0144421 94.953 317 6 3 1 317 1 307 0.0 610
Msa0940130 MtrunA17_Chr3g0144451 54.911 224 100 1 90 312 52 275 4.12e-91 273
Msa0940130 MtrunA17_Chr3g0138231 51.837 245 115 2 69 312 24 266 2.80e-90 270
Msa0940130 MtrunA17_Chr4g0034821 54.464 224 101 1 90 312 68 291 1.26e-89 270
Msa0940130 MtrunA17_Chr4g0034451 41.418 268 144 6 55 312 262 526 7.90e-66 215
Msa0940130 MtrunA17_Chr4g0034731 43.636 220 118 4 97 313 217 433 2.36e-63 206
Msa0940130 MtrunA17_Chr3g0144411 42.466 219 122 2 96 313 324 539 3.30e-63 209
Msa0940130 MtrunA17_Chr3g0144391 41.096 219 125 2 96 313 291 506 4.76e-63 207
Msa0940130 MtrunA17_Chr4g0034721 44.091 220 117 4 97 313 361 577 1.15e-58 197
Msa0940130 MtrunA17_Chr1g0147271 43.243 222 120 4 92 311 166 383 4.58e-58 191
Msa0940130 MtrunA17_Chr4g0034691 43.182 220 119 4 97 313 493 709 2.25e-57 196
Msa0940130 MtrunA17_Chr1g0147321 41.704 223 121 5 94 314 24 239 1.59e-54 178
Msa0940130 MtrunA17_Chr3g0139431 42.478 226 121 6 91 312 101 321 7.19e-54 179
Msa0940130 MtrunA17_Chr1g0147301 41.629 221 122 5 94 311 67 283 9.91e-54 177
Msa0940130 MtrunA17_Chr1g0147291 41.818 220 119 5 94 311 53 265 2.46e-53 176
Msa0940130 MtrunA17_Chr1g0147331 42.601 223 118 7 92 311 108 323 9.70e-53 176
Msa0940130 MtrunA17_Chr1g0147261 40.851 235 127 6 77 310 74 297 1.67e-52 174
Msa0940130 MtrunA17_Chr1g0146631 42.152 223 119 7 92 311 108 323 2.98e-52 174
Msa0940130 MtrunA17_Chr1g0147311 42.105 228 123 6 89 311 131 354 3.80e-52 175
Msa0940130 MtrunA17_Chr1g0147251 40.826 218 119 5 94 310 32 240 2.62e-49 164
Msa0940130 MtrunA17_Chr1g0147241 40.367 218 123 4 94 310 99 310 6.32e-49 166
Msa0940130 MtrunA17_Chr6g0485051 40.000 195 113 4 122 312 11 205 4.35e-48 160
Msa0940130 MtrunA17_Chr8g0365361 30.645 248 165 5 67 311 150 393 1.77e-30 119
Msa0940130 MtrunA17_Chr5g0413591 33.333 216 140 3 97 311 415 627 4.40e-30 120
Msa0940130 MtrunA17_Chr8g0365381 31.276 243 159 5 70 310 404 640 3.06e-29 117
Msa0940130 MtrunA17_Chr8g0365401 33.641 217 138 5 97 311 412 624 5.73e-28 114
Msa0940130 MtrunA17_Chr3g0117791 32.258 217 141 4 97 311 413 625 3.94e-27 111
Msa0940130 MtrunA17_Chr5g0412981 31.193 218 144 5 95 310 195 408 4.09e-26 107
Msa0940130 MtrunA17_Chr5g0412981 30.939 181 120 4 116 294 7 184 1.20e-19 89.0
Msa0940130 MtrunA17_Chr5g0412991 31.193 218 144 5 95 310 195 408 4.47e-26 107
Msa0940130 MtrunA17_Chr5g0412991 30.939 181 120 4 116 294 7 184 9.59e-20 89.0
Msa0940130 MtrunA17_Chr5g0412951 30.457 197 132 4 116 310 14 207 5.75e-24 97.4
Msa0940130 MtrunA17_Chr4g0034051 47.500 80 40 2 236 313 1 80 9.54e-22 87.8
Msa0940130 MtrunA17_Chr4g0076231 33.333 156 100 3 157 310 3 156 3.49e-21 88.6
Msa0940130 MtrunA17_Chr8g0355861 30.973 226 131 8 88 312 59 260 5.17e-21 90.9
Msa0940130 MtrunA17_Chr8g0355331 32.948 173 100 6 140 312 265 421 1.05e-20 92.0
Msa0940130 MtrunA17_Chr8g0355311 35.714 140 81 5 173 312 60 190 3.12e-20 87.0
Msa0940130 MtrunA17_Chr8g0355841 39.286 140 76 5 173 312 379 509 1.62e-19 89.0
Msa0940130 MtrunA17_Chr2g0318901 28.440 218 123 9 97 312 73 259 4.03e-19 85.5
Msa0940130 MtrunA17_Chr8g0355321 35.000 140 82 5 173 312 172 302 6.42e-19 85.5
Msa0940130 MtrunA17_Chr8g0355831 37.857 140 78 5 173 312 221 351 3.50e-18 84.0
Msa0940130 MtrunA17_Chr8g0355871 34.416 154 91 6 161 313 79 223 4.42e-18 82.0
Msa0940130 MtrunA17_Chr1g0168781 32.653 147 91 5 166 312 121 259 1.19e-17 81.3
Msa0940130 MtrunA17_Chr2g0318871 28.899 218 122 10 97 312 73 259 5.63e-16 76.6
Msa0940130 MtrunA17_Chr8g0371951 38.824 85 49 2 219 301 4 87 2.80e-14 67.4
Msa0940130 MtrunA17_Chr5g0413021 29.032 124 84 3 188 310 7 127 4.41e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0940130 AT1G49320.1 47.012 251 123 5 71 311 28 278 3.33e-73 227
Msa0940130 AT5G25610.1 41.350 237 131 5 78 310 157 389 6.69e-56 186
Msa0940130 AT1G70370.2 33.333 222 132 6 97 311 411 623 4.06e-26 108
Msa0940130 AT1G70370.1 33.333 222 132 6 97 311 411 623 4.06e-26 108
Msa0940130 AT1G23760.1 32.883 222 133 7 97 311 407 619 7.47e-25 105
Msa0940130 AT1G60390.1 30.804 224 141 5 93 310 405 620 4.87e-24 102

Find 74 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCACATGGATACTTCTAAA+TGG 0.212561 6_3:-12403034 Msa0940130:CDS
ACATTGTTATCTGTACTATT+TGG 0.233418 6_3:-12404180 Msa0940130:five_prime_UTR
TTTCTTCCCTCCTGATAATC+TGG 0.262557 6_3:-12402949 Msa0940130:CDS
AAACTAGGGAGTTGATTAAT+TGG 0.264198 6_3:+12404070 None:intergenic
CCCTCCTGATAATCTGGTTT+GGG 0.285119 6_3:-12402943 Msa0940130:CDS
GACTTTGCAAATTACATGTT+TGG 0.295983 6_3:-12403293 Msa0940130:CDS
TCCCTCCTGATAATCTGGTT+TGG 0.305714 6_3:-12402944 Msa0940130:CDS
CCGTAAGGGAAAGGAATATA+AGG 0.308964 6_3:+12404030 None:intergenic
ATTAATTGGATGTAGGTCTA+AGG 0.311404 6_3:+12404084 None:intergenic
ATTTCAGTTCCAAATGCAAT+AGG 0.315492 6_3:-12403179 Msa0940130:CDS
TAGAAAGATAACTATAATTA+AGG 0.316435 6_3:-12403774 Msa0940130:CDS
AGCAGTTGATGAATCTTTCT+TGG 0.317340 6_3:+12403511 None:intergenic
GTTCCAAAGGATTTAAAAGT+TGG 0.342461 6_3:-12403557 Msa0940130:CDS
GGCACCCAAACCAGATTATC+AGG 0.351108 6_3:+12402939 None:intergenic
AAGTGTGTATTTCATGGCTT+TGG 0.373004 6_3:+12403385 None:intergenic
TTGTTCAAGTGTGTATTTCA+TGG 0.375445 6_3:+12403379 None:intergenic
TACTACAATTTCATACAAAC+TGG 0.390862 6_3:+12403097 None:intergenic
TTGTATGAAATTGTAGTAGA+AGG 0.391678 6_3:-12403092 Msa0940130:CDS
ATTGTAGTAGAAGGTGAAAA+TGG 0.391681 6_3:-12403083 Msa0940130:CDS
CCTTATATTCCTTTCCCTTA+CGG 0.393008 6_3:-12404030 Msa0940130:five_prime_UTR
TGCTATTCACATGGGAAACT+TGG 0.395953 6_3:+12404110 None:intergenic
AAATTGTGTAATTCTGTAAA+GGG 0.399598 6_3:+12403216 None:intergenic
TGACATACTGGAGAGTTTCC+AGG 0.407905 6_3:+12402972 None:intergenic
AAAAGACTAGTAGGGTATAT+AGG 0.408467 6_3:+12403920 None:intergenic
TCTTTCTAGCACAACTCTCT+TGG 0.411924 6_3:+12403789 None:intergenic
AAAGTCAAACAAAGACTCTA+AGG 0.413155 6_3:+12403310 None:intergenic
TGACAATAGAAAACTGCAAA+AGG 0.418592 6_3:+12403137 None:intergenic
ACAAACTGGTATCACCCTTC+TGG 0.421146 6_3:+12403111 None:intergenic
GGAGAGTGTGTAGGAAAACT+AGG 0.427800 6_3:+12404055 None:intergenic
GAAATTGTGTAATTCTGTAA+AGG 0.428701 6_3:+12403215 None:intergenic
GGGTGACACCCTATTGCATT+TGG 0.430364 6_3:+12403170 None:intergenic
TTGTTATCTGTACTATTTGG+TGG 0.433114 6_3:-12404177 Msa0940130:five_prime_UTR
AGATGGAGAAGAAATCTAGA+AGG 0.448342 6_3:+12403426 None:intergenic
TCACATGGATACTTCTAAAT+GGG 0.449148 6_3:-12403033 Msa0940130:CDS
TTTCAGTTCCAAATGCAATA+GGG 0.450082 6_3:-12403178 Msa0940130:CDS
TATTTCATGGCTTTGGCTTG+TGG 0.464005 6_3:+12403392 None:intergenic
GAGAAGAAATCTAGAAGGAA+AGG 0.485932 6_3:+12403431 None:intergenic
GGAGTTGATTAATTGGATGT+AGG 0.488259 6_3:+12404077 None:intergenic
CGTGTGCTTAATGTAAAACC+TGG 0.488283 6_3:-12402990 Msa0940130:CDS
GGAGGGAAGAAATGACATAC+TGG 0.503691 6_3:+12402960 None:intergenic
TTAATTGGATGTAGGTCTAA+GGG 0.509791 6_3:+12404085 None:intergenic
CTGCAAAAGGGTAAGGCATA+GGG 0.513892 6_3:+12403150 None:intergenic
TGTGCATGATGATTCTCATA+AGG 0.523130 6_3:-12403621 Msa0940130:CDS
TGTAAGCTGTTGCAAATCAT+AGG 0.524928 6_3:+12403745 None:intergenic
TCTAGTAAATAATAGTATCA+TGG 0.525126 6_3:-12403711 Msa0940130:CDS
TGTTATGGTGTTGCAGTCAA+AGG 0.525931 6_3:+12402918 None:intergenic
TAGAAAACTGCAAAAGGGTA+AGG 0.531191 6_3:+12403143 None:intergenic
TTGTAGGTTCCGTAAGGGAA+AGG 0.532944 6_3:+12404021 None:intergenic
GACAATAGAAAACTGCAAAA+GGG 0.537400 6_3:+12403138 None:intergenic
GAGAGTGTGTAGGAAAACTA+GGG 0.550373 6_3:+12404056 None:intergenic
CTAAGGGGTTGCTATTCACA+TGG 0.558604 6_3:+12404101 None:intergenic
GTAGTAGTAGTAGTGCAGTC+AGG 0.560516 6_3:+12404153 None:intergenic
ACTGCAAAAGGGTAAGGCAT+AGG 0.564319 6_3:+12403149 None:intergenic
AGCTGCAGCAATTTGTCACA+TGG 0.567177 6_3:-12403048 Msa0940130:CDS
TTGTGTCAATTAAATTGAGA+CGG 0.570897 6_3:-12404223 Msa0940130:five_prime_UTR
TTTCTATTGTCACAGCCAGA+AGG 0.572783 6_3:-12403126 Msa0940130:CDS
GTAATTCTGTAAAGGGAGAG+AGG 0.580516 6_3:+12403223 None:intergenic
CGTAAGGGAAAGGAATATAA+GGG 0.583432 6_3:+12404031 None:intergenic
CAACAGCTTACAACAGAAGA+GGG 0.584548 6_3:-12403734 Msa0940130:CDS
GCAACAGCTTACAACAGAAG+AGG 0.592350 6_3:-12403735 Msa0940130:CDS
TTCTATTGTCACAGCCAGAA+GGG 0.596892 6_3:-12403125 Msa0940130:CDS
TGCAACACCATAACAAGTTG+TGG 0.599848 6_3:-12402910 Msa0940130:exon
TTTGTTTGTAGGTTCCGTAA+GGG 0.602691 6_3:+12404016 None:intergenic
CTACTACTACTAATATAATG+AGG 0.603146 6_3:-12404140 Msa0940130:five_prime_UTR
TTTATAATATGGGAGGAGGG+AGG 0.603327 6_3:+12402821 None:intergenic
TATAAGGGTGGAGAGTGTGT+AGG 0.610548 6_3:+12404046 None:intergenic
TAATTGGATGTAGGTCTAAG+GGG 0.616446 6_3:+12404086 None:intergenic
ATAGTACAGATAACAATGTG+TGG 0.618170 6_3:+12404184 None:intergenic
GTAGTAGAAGGTGAAAATGG+AGG 0.623165 6_3:-12403080 Msa0940130:CDS
CCCAAACCAGATTATCAGGA+GGG 0.634612 6_3:+12402943 None:intergenic
TGTCAATTAAATTGAGACGG+AGG 0.663260 6_3:-12404220 Msa0940130:five_prime_UTR
ACCCAAACCAGATTATCAGG+AGG 0.664591 6_3:+12402942 None:intergenic
TAAGGGGTTGCTATTCACAT+GGG 0.675323 6_3:+12404102 None:intergenic
AAGGGAAAGGAATATAAGGG+TGG 0.701614 6_3:+12404034 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTCTCTTTTATGAT+TGG - chr6_3:12403204-12403223 Msa0940130:CDS 15.0%
!! TAGAAAGATAACTATAATTA+AGG - chr6_3:12403255-12403274 Msa0940130:CDS 15.0%
!!! TAATTTTTCTCTTTTATGAT+TGG - chr6_3:12403204-12403223 Msa0940130:CDS 15.0%
!! TAGAAAGATAACTATAATTA+AGG - chr6_3:12403255-12403274 Msa0940130:CDS 15.0%
!! TCTAGTAAATAATAGTATCA+TGG - chr6_3:12403318-12403337 Msa0940130:CDS 20.0%
!! TCTTTCTTGGAAAAATAAAT+TGG + chr6_3:12403508-12403527 None:intergenic 20.0%
!!! AAAACTTTGAATTTTGAGTT+GGG + chr6_3:12403763-12403782 None:intergenic 20.0%
!! AAATTGTGTAATTCTGTAAA+GGG + chr6_3:12403816-12403835 None:intergenic 20.0%
!!! TAGTTTTTTTATAATATGGG+AGG + chr6_3:12404218-12404237 None:intergenic 20.0%
!! TCTAGTAAATAATAGTATCA+TGG - chr6_3:12403318-12403337 Msa0940130:CDS 20.0%
!! TCTTTCTTGGAAAAATAAAT+TGG + chr6_3:12403508-12403527 None:intergenic 20.0%
!!! AAAACTTTGAATTTTGAGTT+GGG + chr6_3:12403763-12403782 None:intergenic 20.0%
!! AAATTGTGTAATTCTGTAAA+GGG + chr6_3:12403816-12403835 None:intergenic 20.0%
!!! TAGTTTTTTTATAATATGGG+AGG + chr6_3:12404218-12404237 None:intergenic 20.0%
! TTGTGTCAATTAAATTGAGA+CGG - chr6_3:12402806-12402825 Msa0940130:three_prime_UTR 25.0%
! ACATTGTTATCTGTACTATT+TGG - chr6_3:12402849-12402868 Msa0940130:three_prime_UTR 25.0%
! CTACTACTACTAATATAATG+AGG - chr6_3:12402889-12402908 Msa0940130:three_prime_UTR 25.0%
!!! ATTTTTGGTGTTTTGTTTGT+AGG + chr6_3:12403027-12403046 None:intergenic 25.0%
! CAAACAAAACACCAAAAATA+AGG - chr6_3:12403028-12403047 Msa0940130:CDS 25.0%
! ACAGAAAAAGATCATTTCAA+AGG - chr6_3:12403384-12403403 Msa0940130:CDS 25.0%
!! TTTCCAACTTTTAAATCCTT+TGG + chr6_3:12403478-12403497 None:intergenic 25.0%
!!! ATTTTGTTAGCTTCTTCTTT+TGG + chr6_3:12403556-12403575 None:intergenic 25.0%
!!! CAAAACTTTGAATTTTGAGT+TGG + chr6_3:12403764-12403783 None:intergenic 25.0%
! GAAATTGTGTAATTCTGTAA+AGG + chr6_3:12403817-12403836 None:intergenic 25.0%
! TACTACAATTTCATACAAAC+TGG + chr6_3:12403935-12403954 None:intergenic 25.0%
! TTGTATGAAATTGTAGTAGA+AGG - chr6_3:12403937-12403956 Msa0940130:CDS 25.0%
!!! TTTTTTATAATATGGGAGGA+GGG + chr6_3:12404214-12404233 None:intergenic 25.0%
!!! TTTTTTTATAATATGGGAGG+AGG + chr6_3:12404215-12404234 None:intergenic 25.0%
! TTGTGTCAATTAAATTGAGA+CGG - chr6_3:12402806-12402825 Msa0940130:three_prime_UTR 25.0%
! ACATTGTTATCTGTACTATT+TGG - chr6_3:12402849-12402868 Msa0940130:three_prime_UTR 25.0%
! CTACTACTACTAATATAATG+AGG - chr6_3:12402889-12402908 Msa0940130:three_prime_UTR 25.0%
!!! ATTTTTGGTGTTTTGTTTGT+AGG + chr6_3:12403027-12403046 None:intergenic 25.0%
! CAAACAAAACACCAAAAATA+AGG - chr6_3:12403028-12403047 Msa0940130:CDS 25.0%
! ACAGAAAAAGATCATTTCAA+AGG - chr6_3:12403384-12403403 Msa0940130:CDS 25.0%
!! TTTCCAACTTTTAAATCCTT+TGG + chr6_3:12403478-12403497 None:intergenic 25.0%
!!! ATTTTGTTAGCTTCTTCTTT+TGG + chr6_3:12403556-12403575 None:intergenic 25.0%
!!! CAAAACTTTGAATTTTGAGT+TGG + chr6_3:12403764-12403783 None:intergenic 25.0%
! GAAATTGTGTAATTCTGTAA+AGG + chr6_3:12403817-12403836 None:intergenic 25.0%
! TACTACAATTTCATACAAAC+TGG + chr6_3:12403935-12403954 None:intergenic 25.0%
! TTGTATGAAATTGTAGTAGA+AGG - chr6_3:12403937-12403956 Msa0940130:CDS 25.0%
!!! TTTTTTATAATATGGGAGGA+GGG + chr6_3:12404214-12404233 None:intergenic 25.0%
!!! TTTTTTTATAATATGGGAGG+AGG + chr6_3:12404215-12404234 None:intergenic 25.0%
ATAGTACAGATAACAATGTG+TGG + chr6_3:12402848-12402867 None:intergenic 30.0%
TTGTTATCTGTACTATTTGG+TGG - chr6_3:12402852-12402871 Msa0940130:three_prime_UTR 30.0%
TTAATTGGATGTAGGTCTAA+GGG + chr6_3:12402947-12402966 None:intergenic 30.0%
ATTAATTGGATGTAGGTCTA+AGG + chr6_3:12402948-12402967 None:intergenic 30.0%
! AAACTAGGGAGTTGATTAAT+TGG + chr6_3:12402962-12402981 None:intergenic 30.0%
!!! GAAACTTAGGACCTTATTTT+TGG + chr6_3:12403042-12403061 None:intergenic 30.0%
!!! CTCTGTTTCTTTTGAAACTT+AGG + chr6_3:12403055-12403074 None:intergenic 30.0%
! TGAGACTTTTAATCTCTTCT+TGG - chr6_3:12403078-12403097 Msa0940130:CDS 30.0%
AAAAGACTAGTAGGGTATAT+AGG + chr6_3:12403112-12403131 None:intergenic 30.0%
!!! ATATACCCTACTAGTCTTTT+TGG - chr6_3:12403112-12403131 Msa0940130:CDS 30.0%
TCTTACCAAAAAGACTAGTA+GGG + chr6_3:12403120-12403139 None:intergenic 30.0%
ATCTTACCAAAAAGACTAGT+AGG + chr6_3:12403121-12403140 None:intergenic 30.0%
GTTCCAAAGGATTTAAAAGT+TGG - chr6_3:12403472-12403491 Msa0940130:CDS 30.0%
! TCTTCTTTTGGTAGGAATTT+TGG + chr6_3:12403544-12403563 None:intergenic 30.0%
!!! GGTAGTTTTTTTTGTGTGAA+AGG + chr6_3:12403580-12403599 None:intergenic 30.0%
TTGTTCAAGTGTGTATTTCA+TGG + chr6_3:12403653-12403672 None:intergenic 30.0%
! ACAATGCAATTTTGAACCTA+TGG - chr6_3:12403669-12403688 Msa0940130:CDS 30.0%
AAAGTCAAACAAAGACTCTA+AGG + chr6_3:12403722-12403741 None:intergenic 30.0%
GACTTTGCAAATTACATGTT+TGG - chr6_3:12403736-12403755 Msa0940130:CDS 30.0%
ATTTCAGTTCCAAATGCAAT+AGG - chr6_3:12403850-12403869 Msa0940130:intron 30.0%
TTTCAGTTCCAAATGCAATA+GGG - chr6_3:12403851-12403870 Msa0940130:intron 30.0%
GACAATAGAAAACTGCAAAA+GGG + chr6_3:12403894-12403913 None:intergenic 30.0%
TGACAATAGAAAACTGCAAA+AGG + chr6_3:12403895-12403914 None:intergenic 30.0%
ATTGTAGTAGAAGGTGAAAA+TGG - chr6_3:12403946-12403965 Msa0940130:CDS 30.0%
TCACATGGATACTTCTAAAT+GGG - chr6_3:12403996-12404015 Msa0940130:five_prime_UTR 30.0%
! TTTTACATTAAGCACACGAA+AGG + chr6_3:12404037-12404056 None:intergenic 30.0%
!! AAAAAGACCACAACTTGTTA+TGG + chr6_3:12404129-12404148 None:intergenic 30.0%
ATAGTACAGATAACAATGTG+TGG + chr6_3:12402848-12402867 None:intergenic 30.0%
TTGTTATCTGTACTATTTGG+TGG - chr6_3:12402852-12402871 Msa0940130:three_prime_UTR 30.0%
TTAATTGGATGTAGGTCTAA+GGG + chr6_3:12402947-12402966 None:intergenic 30.0%
ATTAATTGGATGTAGGTCTA+AGG + chr6_3:12402948-12402967 None:intergenic 30.0%
! AAACTAGGGAGTTGATTAAT+TGG + chr6_3:12402962-12402981 None:intergenic 30.0%
!!! GAAACTTAGGACCTTATTTT+TGG + chr6_3:12403042-12403061 None:intergenic 30.0%
!!! CTCTGTTTCTTTTGAAACTT+AGG + chr6_3:12403055-12403074 None:intergenic 30.0%
! TGAGACTTTTAATCTCTTCT+TGG - chr6_3:12403078-12403097 Msa0940130:CDS 30.0%
AAAAGACTAGTAGGGTATAT+AGG + chr6_3:12403112-12403131 None:intergenic 30.0%
!!! ATATACCCTACTAGTCTTTT+TGG - chr6_3:12403112-12403131 Msa0940130:CDS 30.0%
TCTTACCAAAAAGACTAGTA+GGG + chr6_3:12403120-12403139 None:intergenic 30.0%
ATCTTACCAAAAAGACTAGT+AGG + chr6_3:12403121-12403140 None:intergenic 30.0%
GTTCCAAAGGATTTAAAAGT+TGG - chr6_3:12403472-12403491 Msa0940130:CDS 30.0%
! TCTTCTTTTGGTAGGAATTT+TGG + chr6_3:12403544-12403563 None:intergenic 30.0%
!!! GGTAGTTTTTTTTGTGTGAA+AGG + chr6_3:12403580-12403599 None:intergenic 30.0%
TTGTTCAAGTGTGTATTTCA+TGG + chr6_3:12403653-12403672 None:intergenic 30.0%
! ACAATGCAATTTTGAACCTA+TGG - chr6_3:12403669-12403688 Msa0940130:CDS 30.0%
AAAGTCAAACAAAGACTCTA+AGG + chr6_3:12403722-12403741 None:intergenic 30.0%
GACTTTGCAAATTACATGTT+TGG - chr6_3:12403736-12403755 Msa0940130:CDS 30.0%
ATTTCAGTTCCAAATGCAAT+AGG - chr6_3:12403850-12403869 Msa0940130:intron 30.0%
TTTCAGTTCCAAATGCAATA+GGG - chr6_3:12403851-12403870 Msa0940130:intron 30.0%
GACAATAGAAAACTGCAAAA+GGG + chr6_3:12403894-12403913 None:intergenic 30.0%
TGACAATAGAAAACTGCAAA+AGG + chr6_3:12403895-12403914 None:intergenic 30.0%
ATTGTAGTAGAAGGTGAAAA+TGG - chr6_3:12403946-12403965 Msa0940130:CDS 30.0%
TCACATGGATACTTCTAAAT+GGG - chr6_3:12403996-12404015 Msa0940130:five_prime_UTR 30.0%
! TTTTACATTAAGCACACGAA+AGG + chr6_3:12404037-12404056 None:intergenic 30.0%
!! AAAAAGACCACAACTTGTTA+TGG + chr6_3:12404129-12404148 None:intergenic 30.0%
TGTCAATTAAATTGAGACGG+AGG - chr6_3:12402809-12402828 Msa0940130:three_prime_UTR 35.0%
TAATTGGATGTAGGTCTAAG+GGG + chr6_3:12402946-12402965 None:intergenic 35.0%
! GGAGTTGATTAATTGGATGT+AGG + chr6_3:12402955-12402974 None:intergenic 35.0%
CGTAAGGGAAAGGAATATAA+GGG + chr6_3:12403001-12403020 None:intergenic 35.0%
CCTTATATTCCTTTCCCTTA+CGG - chr6_3:12402999-12403018 Msa0940130:CDS 35.0%
TTTGTTTGTAGGTTCCGTAA+GGG + chr6_3:12403016-12403035 None:intergenic 35.0%
!! TTTTGTTTGTAGGTTCCGTA+AGG + chr6_3:12403017-12403036 None:intergenic 35.0%
TGTAAGCTGTTGCAAATCAT+AGG + chr6_3:12403287-12403306 None:intergenic 35.0%
TGTGCATGATGATTCTCATA+AGG - chr6_3:12403408-12403427 Msa0940130:CDS 35.0%
CGAAAAACACGTTCAATTCA+GGG + chr6_3:12403453-12403472 None:intergenic 35.0%
ACGAAAAACACGTTCAATTC+AGG + chr6_3:12403454-12403473 None:intergenic 35.0%
!! AGCAGTTGATGAATCTTTCT+TGG + chr6_3:12403521-12403540 None:intergenic 35.0%
!! TGTTAGCTTCTTCTTTTGGT+AGG + chr6_3:12403552-12403571 None:intergenic 35.0%
GAGAAGAAATCTAGAAGGAA+AGG + chr6_3:12403601-12403620 None:intergenic 35.0%
AGATGGAGAAGAAATCTAGA+AGG + chr6_3:12403606-12403625 None:intergenic 35.0%
!!! TTGTGGAGAGTTTTTTGAGA+TGG + chr6_3:12403623-12403642 None:intergenic 35.0%
AAGTGTGTATTTCATGGCTT+TGG + chr6_3:12403647-12403666 None:intergenic 35.0%
! TGCAATTTTGAACCTATGGA+AGG - chr6_3:12403673-12403692 Msa0940130:CDS 35.0%
! AATTTTGTCTCTCCTTCCAT+AGG + chr6_3:12403688-12403707 None:intergenic 35.0%
TAGAAAACTGCAAAAGGGTA+AGG + chr6_3:12403889-12403908 None:intergenic 35.0%
GTCACATGGATACTTCTAAA+TGG - chr6_3:12403995-12404014 Msa0940130:five_prime_UTR 35.0%
TGTCAATTAAATTGAGACGG+AGG - chr6_3:12402809-12402828 Msa0940130:three_prime_UTR 35.0%
TAATTGGATGTAGGTCTAAG+GGG + chr6_3:12402946-12402965 None:intergenic 35.0%
! GGAGTTGATTAATTGGATGT+AGG + chr6_3:12402955-12402974 None:intergenic 35.0%
CGTAAGGGAAAGGAATATAA+GGG + chr6_3:12403001-12403020 None:intergenic 35.0%
CCTTATATTCCTTTCCCTTA+CGG - chr6_3:12402999-12403018 Msa0940130:CDS 35.0%
TTTGTTTGTAGGTTCCGTAA+GGG + chr6_3:12403016-12403035 None:intergenic 35.0%
!! TTTTGTTTGTAGGTTCCGTA+AGG + chr6_3:12403017-12403036 None:intergenic 35.0%
TGTAAGCTGTTGCAAATCAT+AGG + chr6_3:12403287-12403306 None:intergenic 35.0%
TGTGCATGATGATTCTCATA+AGG - chr6_3:12403408-12403427 Msa0940130:CDS 35.0%
CGAAAAACACGTTCAATTCA+GGG + chr6_3:12403453-12403472 None:intergenic 35.0%
ACGAAAAACACGTTCAATTC+AGG + chr6_3:12403454-12403473 None:intergenic 35.0%
!! AGCAGTTGATGAATCTTTCT+TGG + chr6_3:12403521-12403540 None:intergenic 35.0%
!! TGTTAGCTTCTTCTTTTGGT+AGG + chr6_3:12403552-12403571 None:intergenic 35.0%
GAGAAGAAATCTAGAAGGAA+AGG + chr6_3:12403601-12403620 None:intergenic 35.0%
AGATGGAGAAGAAATCTAGA+AGG + chr6_3:12403606-12403625 None:intergenic 35.0%
!!! TTGTGGAGAGTTTTTTGAGA+TGG + chr6_3:12403623-12403642 None:intergenic 35.0%
AAGTGTGTATTTCATGGCTT+TGG + chr6_3:12403647-12403666 None:intergenic 35.0%
! TGCAATTTTGAACCTATGGA+AGG - chr6_3:12403673-12403692 Msa0940130:CDS 35.0%
! AATTTTGTCTCTCCTTCCAT+AGG + chr6_3:12403688-12403707 None:intergenic 35.0%
TAGAAAACTGCAAAAGGGTA+AGG + chr6_3:12403889-12403908 None:intergenic 35.0%
GTCACATGGATACTTCTAAA+TGG - chr6_3:12403995-12404014 Msa0940130:five_prime_UTR 35.0%
TGCTATTCACATGGGAAACT+TGG + chr6_3:12402922-12402941 None:intergenic 40.0%
! TAAGGGGTTGCTATTCACAT+GGG + chr6_3:12402930-12402949 None:intergenic 40.0%
! GAGAGTGTGTAGGAAAACTA+GGG + chr6_3:12402976-12402995 None:intergenic 40.0%
AAGGGAAAGGAATATAAGGG+TGG + chr6_3:12402998-12403017 None:intergenic 40.0%
CCGTAAGGGAAAGGAATATA+AGG + chr6_3:12403002-12403021 None:intergenic 40.0%
! TCTTTCTAGCACAACTCTCT+TGG + chr6_3:12403243-12403262 None:intergenic 40.0%
CAACAGCTTACAACAGAAGA+GGG - chr6_3:12403295-12403314 Msa0940130:CDS 40.0%
! GAACGTGTTTTTCGTTCCAA+AGG - chr6_3:12403459-12403478 Msa0940130:CDS 40.0%
!! TATTTCATGGCTTTGGCTTG+TGG + chr6_3:12403640-12403659 None:intergenic 40.0%
! GAGAGAGGATTTTGTGACAT+GGG + chr6_3:12403794-12403813 None:intergenic 40.0%
GTAATTCTGTAAAGGGAGAG+AGG + chr6_3:12403809-12403828 None:intergenic 40.0%
TTTCTATTGTCACAGCCAGA+AGG - chr6_3:12403903-12403922 Msa0940130:intron 40.0%
TTCTATTGTCACAGCCAGAA+GGG - chr6_3:12403904-12403923 Msa0940130:intron 40.0%
GTAGTAGAAGGTGAAAATGG+AGG - chr6_3:12403949-12403968 Msa0940130:CDS 40.0%
CGTGTGCTTAATGTAAAACC+TGG - chr6_3:12404039-12404058 Msa0940130:five_prime_UTR 40.0%
TTTCTTCCCTCCTGATAATC+TGG - chr6_3:12404080-12404099 Msa0940130:five_prime_UTR 40.0%
!! TGTTATGGTGTTGCAGTCAA+AGG + chr6_3:12404114-12404133 None:intergenic 40.0%
TGCAACACCATAACAAGTTG+TGG - chr6_3:12404119-12404138 Msa0940130:five_prime_UTR 40.0%
TTTATAATATGGGAGGAGGG+AGG + chr6_3:12404211-12404230 None:intergenic 40.0%
TGCTATTCACATGGGAAACT+TGG + chr6_3:12402922-12402941 None:intergenic 40.0%
! TAAGGGGTTGCTATTCACAT+GGG + chr6_3:12402930-12402949 None:intergenic 40.0%
! GAGAGTGTGTAGGAAAACTA+GGG + chr6_3:12402976-12402995 None:intergenic 40.0%
AAGGGAAAGGAATATAAGGG+TGG + chr6_3:12402998-12403017 None:intergenic 40.0%
CCGTAAGGGAAAGGAATATA+AGG + chr6_3:12403002-12403021 None:intergenic 40.0%
! TCTTTCTAGCACAACTCTCT+TGG + chr6_3:12403243-12403262 None:intergenic 40.0%
CAACAGCTTACAACAGAAGA+GGG - chr6_3:12403295-12403314 Msa0940130:CDS 40.0%
! GAACGTGTTTTTCGTTCCAA+AGG - chr6_3:12403459-12403478 Msa0940130:CDS 40.0%
!! TATTTCATGGCTTTGGCTTG+TGG + chr6_3:12403640-12403659 None:intergenic 40.0%
! GAGAGAGGATTTTGTGACAT+GGG + chr6_3:12403794-12403813 None:intergenic 40.0%
GTAATTCTGTAAAGGGAGAG+AGG + chr6_3:12403809-12403828 None:intergenic 40.0%
TTTCTATTGTCACAGCCAGA+AGG - chr6_3:12403903-12403922 Msa0940130:intron 40.0%
TTCTATTGTCACAGCCAGAA+GGG - chr6_3:12403904-12403923 Msa0940130:intron 40.0%
GTAGTAGAAGGTGAAAATGG+AGG - chr6_3:12403949-12403968 Msa0940130:CDS 40.0%
CGTGTGCTTAATGTAAAACC+TGG - chr6_3:12404039-12404058 Msa0940130:five_prime_UTR 40.0%
TTTCTTCCCTCCTGATAATC+TGG - chr6_3:12404080-12404099 Msa0940130:five_prime_UTR 40.0%
!! TGTTATGGTGTTGCAGTCAA+AGG + chr6_3:12404114-12404133 None:intergenic 40.0%
TGCAACACCATAACAAGTTG+TGG - chr6_3:12404119-12404138 Msa0940130:five_prime_UTR 40.0%
TTTATAATATGGGAGGAGGG+AGG + chr6_3:12404211-12404230 None:intergenic 40.0%
GTAGTAGTAGTAGTGCAGTC+AGG + chr6_3:12402879-12402898 None:intergenic 45.0%
! CTAAGGGGTTGCTATTCACA+TGG + chr6_3:12402931-12402950 None:intergenic 45.0%
! GGAGAGTGTGTAGGAAAACT+AGG + chr6_3:12402977-12402996 None:intergenic 45.0%
! TATAAGGGTGGAGAGTGTGT+AGG + chr6_3:12402986-12403005 None:intergenic 45.0%
TTGTAGGTTCCGTAAGGGAA+AGG + chr6_3:12403011-12403030 None:intergenic 45.0%
GCAACAGCTTACAACAGAAG+AGG - chr6_3:12403294-12403313 Msa0940130:CDS 45.0%
!! GGATTTTGTGACATGGGTTG+TGG + chr6_3:12403788-12403807 None:intergenic 45.0%
! GGAGAGAGGATTTTGTGACA+TGG + chr6_3:12403795-12403814 None:intergenic 45.0%
CTGCAAAAGGGTAAGGCATA+GGG + chr6_3:12403882-12403901 None:intergenic 45.0%
ACTGCAAAAGGGTAAGGCAT+AGG + chr6_3:12403883-12403902 None:intergenic 45.0%
ACAAACTGGTATCACCCTTC+TGG + chr6_3:12403921-12403940 None:intergenic 45.0%
AGCTGCAGCAATTTGTCACA+TGG - chr6_3:12403981-12404000 Msa0940130:five_prime_UTR 45.0%
TGACATACTGGAGAGTTTCC+AGG + chr6_3:12404060-12404079 None:intergenic 45.0%
GGAGGGAAGAAATGACATAC+TGG + chr6_3:12404072-12404091 None:intergenic 45.0%
TCCCTCCTGATAATCTGGTT+TGG - chr6_3:12404085-12404104 Msa0940130:five_prime_UTR 45.0%
CCCAAACCAGATTATCAGGA+GGG + chr6_3:12404089-12404108 None:intergenic 45.0%
! CCCTCCTGATAATCTGGTTT+GGG - chr6_3:12404086-12404105 Msa0940130:five_prime_UTR 45.0%
ACCCAAACCAGATTATCAGG+AGG + chr6_3:12404090-12404109 None:intergenic 45.0%
GTAGTAGTAGTAGTGCAGTC+AGG + chr6_3:12402879-12402898 None:intergenic 45.0%
! CTAAGGGGTTGCTATTCACA+TGG + chr6_3:12402931-12402950 None:intergenic 45.0%
! GGAGAGTGTGTAGGAAAACT+AGG + chr6_3:12402977-12402996 None:intergenic 45.0%
! TATAAGGGTGGAGAGTGTGT+AGG + chr6_3:12402986-12403005 None:intergenic 45.0%
TTGTAGGTTCCGTAAGGGAA+AGG + chr6_3:12403011-12403030 None:intergenic 45.0%
GCAACAGCTTACAACAGAAG+AGG - chr6_3:12403294-12403313 Msa0940130:CDS 45.0%
!! GGATTTTGTGACATGGGTTG+TGG + chr6_3:12403788-12403807 None:intergenic 45.0%
! GGAGAGAGGATTTTGTGACA+TGG + chr6_3:12403795-12403814 None:intergenic 45.0%
CTGCAAAAGGGTAAGGCATA+GGG + chr6_3:12403882-12403901 None:intergenic 45.0%
ACTGCAAAAGGGTAAGGCAT+AGG + chr6_3:12403883-12403902 None:intergenic 45.0%
ACAAACTGGTATCACCCTTC+TGG + chr6_3:12403921-12403940 None:intergenic 45.0%
AGCTGCAGCAATTTGTCACA+TGG - chr6_3:12403981-12404000 Msa0940130:five_prime_UTR 45.0%
TGACATACTGGAGAGTTTCC+AGG + chr6_3:12404060-12404079 None:intergenic 45.0%
GGAGGGAAGAAATGACATAC+TGG + chr6_3:12404072-12404091 None:intergenic 45.0%
TCCCTCCTGATAATCTGGTT+TGG - chr6_3:12404085-12404104 Msa0940130:five_prime_UTR 45.0%
CCCAAACCAGATTATCAGGA+GGG + chr6_3:12404089-12404108 None:intergenic 45.0%
! CCCTCCTGATAATCTGGTTT+GGG - chr6_3:12404086-12404105 Msa0940130:five_prime_UTR 45.0%
ACCCAAACCAGATTATCAGG+AGG + chr6_3:12404090-12404109 None:intergenic 45.0%
!! GTATTATATATATATATATT+TGG - chr6_3:12404161-12404180 Msa0940130:five_prime_UTR 5.0%
!!! TTTTAGTTTTTTTATAATAT+GGG + chr6_3:12404221-12404240 None:intergenic 5.0%
!!! TTTTTAGTTTTTTTATAATA+TGG + chr6_3:12404222-12404241 None:intergenic 5.0%
!! GTATTATATATATATATATT+TGG - chr6_3:12404161-12404180 Msa0940130:five_prime_UTR 5.0%
!!! TTTTAGTTTTTTTATAATAT+GGG + chr6_3:12404221-12404240 None:intergenic 5.0%
!!! TTTTTAGTTTTTTTATAATA+TGG + chr6_3:12404222-12404241 None:intergenic 5.0%
GGGTGACACCCTATTGCATT+TGG + chr6_3:12403862-12403881 None:intergenic 50.0%
GGCACCCAAACCAGATTATC+AGG + chr6_3:12404093-12404112 None:intergenic 50.0%
GGGTGACACCCTATTGCATT+TGG + chr6_3:12403862-12403881 None:intergenic 50.0%
GGCACCCAAACCAGATTATC+AGG + chr6_3:12404093-12404112 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 12402797 12404254 12402797 ID=Msa0940130;Name=Msa0940130
chr6_3 mRNA 12402797 12404254 12402797 ID=Msa0940130-mRNA-1;Parent=Msa0940130;Name=Msa0940130-mRNA-1;_AED=0.03;_eAED=0.03;_QI=280|1|1|1|1|1|2|123|317
chr6_3 exon 12402797 12403816 12402797 ID=Msa0940130-mRNA-1:exon:4094;Parent=Msa0940130-mRNA-1
chr6_3 exon 12403918 12404254 12403918 ID=Msa0940130-mRNA-1:exon:4093;Parent=Msa0940130-mRNA-1
chr6_3 five_prime_UTR 12403975 12404254 12403975 ID=Msa0940130-mRNA-1:five_prime_utr;Parent=Msa0940130-mRNA-1
chr6_3 CDS 12403918 12403974 12403918 ID=Msa0940130-mRNA-1:cds;Parent=Msa0940130-mRNA-1
chr6_3 CDS 12402920 12403816 12402920 ID=Msa0940130-mRNA-1:cds;Parent=Msa0940130-mRNA-1
chr6_3 three_prime_UTR 12402797 12402919 12402797 ID=Msa0940130-mRNA-1:three_prime_utr;Parent=Msa0940130-mRNA-1
Gene Sequence

>Msa0940130

ATGAGACTTTTAATCTCTTCTTGGAGCTTCATCCTATATACCCTACTAGTCTTTTTGATTGTCCAAGAGAGTTGTGCTAGAAAGATAACTATAATTAAGGATGCAGCCTATGATTTGCAACAGCTTACAACAGAAGAGGGTCTAGTAAATAATAGTATCATGGAAGTTGAAAACTCTGATCATGTAGAAATGAATAAAAAAGATGAACAGAAAAAGATCATTTCAAAGGATGTGCATGATGATTCTCATAAGGATCATCACAATCAAATAGACCCTGAATTGAACGTGTTTTTCGTTCCAAAGGATTTAAAAGTTGGAAAAATAATGCCAATTTATTTTTCCAAGAAAGATTCATCAACTGCTCCAAAATTCCTACCAAAAGAAGAAGCTAACAAAATTCCTTTCACACAAAAAAAACTACCTTTCCTTCTAGATTTCTTCTCCATCTCAAAAAACTCTCCACAAGCCAAAGCCATGAAATACACACTTGAACAATGCAATTTTGAACCTATGGAAGGAGAGACAAAATTCTGTGCTACATCCTTAGAGTCTTTGTTTGACTTTGCAAATTACATGTTTGGACCCAACTCAAAATTCAAAGTTTTGACCACAACCCATGTCACAAAATCCTCTCTCCCTTTACAGAATTACACAATTTCAAAAGTAAATGAAATTTCAGTTCCAAATGCAATAGGGTGTCACCCTATGCCTTACCCTTTTGCAGTTTTCTATTGTCACAGCCAGAAGGGTGATACCAGTTTGTATGAAATTGTAGTAGAAGGTGAAAATGGAGGAATTGTTCAAGCTGCAGCAATTTGTCACATGGATACTTCTAAATGGGATGCAGATCATGTTGCCTTTCGTGTGCTTAATGTAAAACCTGGAAACTCTCCAGTATGTCATTTCTTCCCTCCTGATAATCTGGTTTGGGTGCCTTTGACTGCAACACCATAA
Protein sequence

>Msa0940130

MRLLISSWSFILYTLLVFLIVQESCARKITIIKDAAYDLQQLTTEEGLVNNSIMEVENSDHVEMNKKDEQKKIISKDVHDDSHKDHHNQIDPELNVFFVPKDLKVGKIMPIYFSKKDSSTAPKFLPKEEANKIPFTQKKLPFLLDFFSISKNSPQAKAMKYTLEQCNFEPMEGETKFCATSLESLFDFANYMFGPNSKFKVLTTTHVTKSSLPLQNYTISKVNEISVPNAIGCHPMPYPFAVFYCHSQKGDTSLYEIVVEGENGGIVQAAAICHMDTSKWDADHVAFRVLNVKPGNSPVCHFFPPDNLVWVPLTATP*