AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0978500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0978500 MtrunA17_Chr6g0457391 92.754 69 5 0 45 113 1 69 6.17e-43 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0978500 AT5G16060.1 73.913 69 18 0 45 113 1 69 7.09e-33 110

Find 18 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATCCTCAAAGGTTGAAA+TGG 0.320325 6_4:+14538664 Msa0978500:CDS
GGAGAACACTTTCTGTTGTT+TGG 0.342847 6_4:+14539636 Msa0978500:CDS
ACCACCCATTTCAACCTTTG+AGG 0.417630 6_4:-14538669 None:intergenic
CTGTTCTTCTGAATCCTCAA+AGG 0.445378 6_4:+14538655 Msa0978500:CDS
GAATCCTCAAAGGTTGAAAT+GGG 0.491464 6_4:+14538665 Msa0978500:CDS
ACTTTCAGCTCCTGCGACTG+AGG 0.522971 6_4:+14538627 Msa0978500:CDS
TTTCTGTTGTTTGGCAATGT+CGG 0.528813 6_4:+14539645 Msa0978500:CDS
AAATATGCTGAGTGTGCTAT+GGG 0.534698 6_4:+14539614 Msa0978500:CDS
GCAATGTCGGAAAGTAGCTA+AGG 0.538984 6_4:+14539658 Msa0978500:CDS
GAAATATGCTGAGTGTGCTA+TGG 0.542058 6_4:+14539613 Msa0978500:CDS
AGCTTTACGGAGTAAAATGA+AGG 0.543630 6_4:+14539556 Msa0978500:intron
CAATGTCGGAAAGTAGCTAA+GGG 0.551390 6_4:+14539659 Msa0978500:CDS
AACAGAACAACCTCAGTCGC+AGG 0.566314 6_4:-14538637 None:intergenic
ACGGAGTAAAATGAAGGTGA+AGG 0.589183 6_4:+14539562 Msa0978500:CDS
TCCTCAAAGGTTGAAATGGG+TGG 0.593357 6_4:+14538668 Msa0978500:CDS
GTGAAGAAGAAAGTCGAAGA+AGG 0.598683 6_4:+14538719 Msa0978500:CDS
AATGTCGGAAAGTAGCTAAG+GGG 0.717917 6_4:+14539660 Msa0978500:CDS
AATATGCTGAGTGTGCTATG+GGG 0.810113 6_4:+14539615 Msa0978500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTTTTTTTTTTTTTTTG+AGG - chr6_4:14538570-14538589 None:intergenic 10.0%
!!! TTTGTTTTTTTTTTTTTTTG+AGG - chr6_4:14538570-14538589 None:intergenic 10.0%
!!! TCAATTTTTATGAACAAATA+TGG + chr6_4:14538798-14538817 Msa0978500:intron 15.0%
!!! CAATTTTTATGAACAAATAT+GGG + chr6_4:14538799-14538818 Msa0978500:intron 15.0%
!! TATATACATTAATCCATAAA+TGG + chr6_4:14538845-14538864 Msa0978500:intron 15.0%
!! ATATACATTAATCCATAAAT+GGG + chr6_4:14538846-14538865 Msa0978500:intron 15.0%
!!! GTCATTTTTTAAATTATTAC+CGG + chr6_4:14539306-14539325 Msa0978500:intron 15.0%
!!! TCAATTTTTATGAACAAATA+TGG + chr6_4:14538798-14538817 Msa0978500:intron 15.0%
!!! CAATTTTTATGAACAAATAT+GGG + chr6_4:14538799-14538818 Msa0978500:intron 15.0%
!! TATATACATTAATCCATAAA+TGG + chr6_4:14538845-14538864 Msa0978500:intron 15.0%
!! ATATACATTAATCCATAAAT+GGG + chr6_4:14538846-14538865 Msa0978500:intron 15.0%
!!! GTCATTTTTTAAATTATTAC+CGG + chr6_4:14539306-14539325 Msa0978500:intron 15.0%
!! CTTTATGATGAAGAAAAAAT+GGG - chr6_4:14538776-14538795 None:intergenic 20.0%
!! ACTTTATGATGAAGAAAAAA+TGG - chr6_4:14538777-14538796 None:intergenic 20.0%
!! AAATATGGGTTTATTTCAAA+TGG + chr6_4:14538813-14538832 Msa0978500:intron 20.0%
!! AATATGGGTTTATTTCAAAT+GGG + chr6_4:14538814-14538833 Msa0978500:intron 20.0%
!!! TATCATCATTTTCATTCAAT+TGG + chr6_4:14538917-14538936 Msa0978500:intron 20.0%
!!! ATCATCATTTTCATTCAATT+GGG + chr6_4:14538918-14538937 Msa0978500:intron 20.0%
!! CATATTATTGAAATGGATAA+CGG - chr6_4:14539212-14539231 None:intergenic 20.0%
!!! TAAGAAGCATATTATTGAAA+TGG - chr6_4:14539219-14539238 None:intergenic 20.0%
!! CTTTATGATGAAGAAAAAAT+GGG - chr6_4:14538776-14538795 None:intergenic 20.0%
!! ACTTTATGATGAAGAAAAAA+TGG - chr6_4:14538777-14538796 None:intergenic 20.0%
!! AAATATGGGTTTATTTCAAA+TGG + chr6_4:14538813-14538832 Msa0978500:intron 20.0%
!! AATATGGGTTTATTTCAAAT+GGG + chr6_4:14538814-14538833 Msa0978500:intron 20.0%
!!! TATCATCATTTTCATTCAAT+TGG + chr6_4:14538917-14538936 Msa0978500:intron 20.0%
!!! ATCATCATTTTCATTCAATT+GGG + chr6_4:14538918-14538937 Msa0978500:intron 20.0%
!! CATATTATTGAAATGGATAA+CGG - chr6_4:14539212-14539231 None:intergenic 20.0%
!!! TAAGAAGCATATTATTGAAA+TGG - chr6_4:14539219-14539238 None:intergenic 20.0%
!! AAAAGAAGTTTTCCATTCTT+CGG + chr6_4:14538589-14538608 Msa0978500:CDS 25.0%
! GTTAGGGTTTAGTATTATTA+TGG - chr6_4:14538747-14538766 None:intergenic 25.0%
!!! ATGAAGAAAAAATGGGTTTT+GGG - chr6_4:14538769-14538788 None:intergenic 25.0%
!! CATAATTTGAAACCCATTTA+TGG - chr6_4:14538861-14538880 None:intergenic 25.0%
! GGTTTCAAATTATGATCAAA+TGG + chr6_4:14538867-14538886 Msa0978500:intron 25.0%
!! TTTTCATTCAATTGGGTTTT+AGG + chr6_4:14538925-14538944 Msa0978500:intron 25.0%
! TTATACATAAGTGCTTATCA+TGG - chr6_4:14539000-14539019 None:intergenic 25.0%
!!! TTCATTTTTCGTTGTGTTTT+TGG + chr6_4:14539438-14539457 Msa0978500:intron 25.0%
! ATGATCAAAACATCGTTTAA+TGG + chr6_4:14539499-14539518 Msa0978500:intron 25.0%
!! AAAAGAAGTTTTCCATTCTT+CGG + chr6_4:14538589-14538608 Msa0978500:CDS 25.0%
! GTTAGGGTTTAGTATTATTA+TGG - chr6_4:14538747-14538766 None:intergenic 25.0%
!!! ATGAAGAAAAAATGGGTTTT+GGG - chr6_4:14538769-14538788 None:intergenic 25.0%
!! CATAATTTGAAACCCATTTA+TGG - chr6_4:14538861-14538880 None:intergenic 25.0%
! GGTTTCAAATTATGATCAAA+TGG + chr6_4:14538867-14538886 Msa0978500:intron 25.0%
!! TTTTCATTCAATTGGGTTTT+AGG + chr6_4:14538925-14538944 Msa0978500:intron 25.0%
! TTATACATAAGTGCTTATCA+TGG - chr6_4:14539000-14539019 None:intergenic 25.0%
!!! TTCATTTTTCGTTGTGTTTT+TGG + chr6_4:14539438-14539457 Msa0978500:intron 25.0%
! ATGATCAAAACATCGTTTAA+TGG + chr6_4:14539499-14539518 Msa0978500:intron 25.0%
!!! AAAAAATGGGTTTTGGGTTA+GGG - chr6_4:14538763-14538782 None:intergenic 30.0%
!! GATGAAGAAAAAATGGGTTT+TGG - chr6_4:14538770-14538789 None:intergenic 30.0%
!! GTTTGATAGTGAAATTGATG+TGG + chr6_4:14538889-14538908 Msa0978500:intron 30.0%
CTATAAGCTACAGAAAAAGA+TGG - chr6_4:14538965-14538984 None:intergenic 30.0%
ATAATCACATGTGTTAGAGA+CGG - chr6_4:14539276-14539295 None:intergenic 30.0%
!!! AGATGGGTTTTATTCTAATG+AGG + chr6_4:14539467-14539486 Msa0978500:intron 30.0%
ACATCGTTTAATGGTGATAT+TGG + chr6_4:14539508-14539527 Msa0978500:intron 30.0%
TAATGGTGATATTGGAATGA+TGG + chr6_4:14539516-14539535 Msa0978500:intron 30.0%
AATGGTGATATTGGAATGAT+GGG + chr6_4:14539517-14539536 Msa0978500:intron 30.0%
! TGATCAATTTTGCAGCTTTA+CGG + chr6_4:14539543-14539562 Msa0978500:intron 30.0%
!!! AAAAAATGGGTTTTGGGTTA+GGG - chr6_4:14538763-14538782 None:intergenic 30.0%
!! GATGAAGAAAAAATGGGTTT+TGG - chr6_4:14538770-14538789 None:intergenic 30.0%
!! GTTTGATAGTGAAATTGATG+TGG + chr6_4:14538889-14538908 Msa0978500:intron 30.0%
CTATAAGCTACAGAAAAAGA+TGG - chr6_4:14538965-14538984 None:intergenic 30.0%
ATAATCACATGTGTTAGAGA+CGG - chr6_4:14539276-14539295 None:intergenic 30.0%
!!! AGATGGGTTTTATTCTAATG+AGG + chr6_4:14539467-14539486 Msa0978500:intron 30.0%
ACATCGTTTAATGGTGATAT+TGG + chr6_4:14539508-14539527 Msa0978500:intron 30.0%
TAATGGTGATATTGGAATGA+TGG + chr6_4:14539516-14539535 Msa0978500:intron 30.0%
AATGGTGATATTGGAATGAT+GGG + chr6_4:14539517-14539536 Msa0978500:intron 30.0%
! TGATCAATTTTGCAGCTTTA+CGG + chr6_4:14539543-14539562 Msa0978500:intron 30.0%
! GTTTTCCATTCTTCGGTTTT+TGG + chr6_4:14538596-14538615 Msa0978500:CDS 35.0%
TGAATCCTCAAAGGTTGAAA+TGG + chr6_4:14538664-14538683 Msa0978500:CDS 35.0%
GAATCCTCAAAGGTTGAAAT+GGG + chr6_4:14538665-14538684 Msa0978500:CDS 35.0%
!!! GAAAAAATGGGTTTTGGGTT+AGG - chr6_4:14538764-14538783 None:intergenic 35.0%
! TGATAGTGAAATTGATGTGG+TGG + chr6_4:14538892-14538911 Msa0978500:intron 35.0%
GATAGTGAAATTGATGTGGT+GGG + chr6_4:14538893-14538912 Msa0978500:intron 35.0%
AAAAAGATGGTTGTCCAAAC+GGG - chr6_4:14538952-14538971 None:intergenic 35.0%
GAAAAAGATGGTTGTCCAAA+CGG - chr6_4:14538953-14538972 None:intergenic 35.0%
!! GCTGTTTTCATAAGCTATCT+TGG + chr6_4:14539073-14539092 Msa0978500:intron 35.0%
ATCTGAGCTTATCTACTGTT+TGG - chr6_4:14539172-14539191 None:intergenic 35.0%
! GAAATGGATAACGGTATGTT+TGG - chr6_4:14539203-14539222 None:intergenic 35.0%
!!! TGTGTTTTTGGAAGAAGAGA+TGG + chr6_4:14539450-14539469 Msa0978500:intron 35.0%
!!! GTGTTTTTGGAAGAAGAGAT+GGG + chr6_4:14539451-14539470 Msa0978500:intron 35.0%
AGCTTTACGGAGTAAAATGA+AGG + chr6_4:14539556-14539575 Msa0978500:intron 35.0%
AAATATGCTGAGTGTGCTAT+GGG + chr6_4:14539614-14539633 Msa0978500:CDS 35.0%
! TTTCTGTTGTTTGGCAATGT+CGG + chr6_4:14539645-14539664 Msa0978500:CDS 35.0%
! GTTTTCCATTCTTCGGTTTT+TGG + chr6_4:14538596-14538615 Msa0978500:CDS 35.0%
TGAATCCTCAAAGGTTGAAA+TGG + chr6_4:14538664-14538683 Msa0978500:CDS 35.0%
GAATCCTCAAAGGTTGAAAT+GGG + chr6_4:14538665-14538684 Msa0978500:CDS 35.0%
!!! GAAAAAATGGGTTTTGGGTT+AGG - chr6_4:14538764-14538783 None:intergenic 35.0%
! TGATAGTGAAATTGATGTGG+TGG + chr6_4:14538892-14538911 Msa0978500:intron 35.0%
GATAGTGAAATTGATGTGGT+GGG + chr6_4:14538893-14538912 Msa0978500:intron 35.0%
AAAAAGATGGTTGTCCAAAC+GGG - chr6_4:14538952-14538971 None:intergenic 35.0%
GAAAAAGATGGTTGTCCAAA+CGG - chr6_4:14538953-14538972 None:intergenic 35.0%
!! GCTGTTTTCATAAGCTATCT+TGG + chr6_4:14539073-14539092 Msa0978500:intron 35.0%
ATCTGAGCTTATCTACTGTT+TGG - chr6_4:14539172-14539191 None:intergenic 35.0%
! GAAATGGATAACGGTATGTT+TGG - chr6_4:14539203-14539222 None:intergenic 35.0%
!!! TGTGTTTTTGGAAGAAGAGA+TGG + chr6_4:14539450-14539469 Msa0978500:intron 35.0%
!!! GTGTTTTTGGAAGAAGAGAT+GGG + chr6_4:14539451-14539470 Msa0978500:intron 35.0%
AGCTTTACGGAGTAAAATGA+AGG + chr6_4:14539556-14539575 Msa0978500:intron 35.0%
AAATATGCTGAGTGTGCTAT+GGG + chr6_4:14539614-14539633 Msa0978500:CDS 35.0%
! TTTCTGTTGTTTGGCAATGT+CGG + chr6_4:14539645-14539664 Msa0978500:CDS 35.0%
AACAGCCAAAAACCGAAGAA+TGG - chr6_4:14538604-14538623 None:intergenic 40.0%
CTGTTCTTCTGAATCCTCAA+AGG + chr6_4:14538655-14538674 Msa0978500:CDS 40.0%
GTGAAGAAGAAAGTCGAAGA+AGG + chr6_4:14538719-14538738 Msa0978500:CDS 40.0%
AAAAGATGGTTGTCCAAACG+GGG - chr6_4:14538951-14538970 None:intergenic 40.0%
! ACGCCTTCTATCAAAAGTGT+CGG - chr6_4:14539245-14539264 None:intergenic 40.0%
TCATAGATCAATGTCGTGTC+TGG + chr6_4:14539371-14539390 Msa0978500:intron 40.0%
ACGGAGTAAAATGAAGGTGA+AGG + chr6_4:14539562-14539581 Msa0978500:CDS 40.0%
GAAATATGCTGAGTGTGCTA+TGG + chr6_4:14539613-14539632 Msa0978500:CDS 40.0%
AATATGCTGAGTGTGCTATG+GGG + chr6_4:14539615-14539634 Msa0978500:CDS 40.0%
GGAGAACACTTTCTGTTGTT+TGG + chr6_4:14539636-14539655 Msa0978500:CDS 40.0%
CAATGTCGGAAAGTAGCTAA+GGG + chr6_4:14539659-14539678 Msa0978500:CDS 40.0%
AATGTCGGAAAGTAGCTAAG+GGG + chr6_4:14539660-14539679 Msa0978500:CDS 40.0%
AACAGCCAAAAACCGAAGAA+TGG - chr6_4:14538604-14538623 None:intergenic 40.0%
CTGTTCTTCTGAATCCTCAA+AGG + chr6_4:14538655-14538674 Msa0978500:CDS 40.0%
GTGAAGAAGAAAGTCGAAGA+AGG + chr6_4:14538719-14538738 Msa0978500:CDS 40.0%
AAAAGATGGTTGTCCAAACG+GGG - chr6_4:14538951-14538970 None:intergenic 40.0%
! ACGCCTTCTATCAAAAGTGT+CGG - chr6_4:14539245-14539264 None:intergenic 40.0%
TCATAGATCAATGTCGTGTC+TGG + chr6_4:14539371-14539390 Msa0978500:intron 40.0%
ACGGAGTAAAATGAAGGTGA+AGG + chr6_4:14539562-14539581 Msa0978500:CDS 40.0%
GAAATATGCTGAGTGTGCTA+TGG + chr6_4:14539613-14539632 Msa0978500:CDS 40.0%
AATATGCTGAGTGTGCTATG+GGG + chr6_4:14539615-14539634 Msa0978500:CDS 40.0%
GGAGAACACTTTCTGTTGTT+TGG + chr6_4:14539636-14539655 Msa0978500:CDS 40.0%
CAATGTCGGAAAGTAGCTAA+GGG + chr6_4:14539659-14539678 Msa0978500:CDS 40.0%
AATGTCGGAAAGTAGCTAAG+GGG + chr6_4:14539660-14539679 Msa0978500:CDS 40.0%
TCCTCAAAGGTTGAAATGGG+TGG + chr6_4:14538668-14538687 Msa0978500:CDS 45.0%
ACCACCCATTTCAACCTTTG+AGG - chr6_4:14538672-14538691 None:intergenic 45.0%
!! GCACCGACACTTTTGATAGA+AGG + chr6_4:14539239-14539258 Msa0978500:intron 45.0%
CGACATTGACACAGAAACAC+CGG - chr6_4:14539328-14539347 None:intergenic 45.0%
TTCTGTGTCAATGTCGTGTC+TGG + chr6_4:14539332-14539351 Msa0978500:intron 45.0%
GCAATGTCGGAAAGTAGCTA+AGG + chr6_4:14539658-14539677 Msa0978500:CDS 45.0%
TCCTCAAAGGTTGAAATGGG+TGG + chr6_4:14538668-14538687 Msa0978500:CDS 45.0%
ACCACCCATTTCAACCTTTG+AGG - chr6_4:14538672-14538691 None:intergenic 45.0%
!! GCACCGACACTTTTGATAGA+AGG + chr6_4:14539239-14539258 Msa0978500:intron 45.0%
CGACATTGACACAGAAACAC+CGG - chr6_4:14539328-14539347 None:intergenic 45.0%
TTCTGTGTCAATGTCGTGTC+TGG + chr6_4:14539332-14539351 Msa0978500:intron 45.0%
GCAATGTCGGAAAGTAGCTA+AGG + chr6_4:14539658-14539677 Msa0978500:CDS 45.0%
AACAGAACAACCTCAGTCGC+AGG - chr6_4:14538640-14538659 None:intergenic 50.0%
!!! ATTGGGTTTTAGGCCCCGTT+TGG + chr6_4:14538935-14538954 Msa0978500:intron 50.0%
GTCGTGTCTGGTGTTTGTGT+CGG + chr6_4:14539344-14539363 Msa0978500:intron 50.0%
GTCGTGTCTGGTGTTTGTGT+CGG + chr6_4:14539344-14539363 Msa0978500:intron 50.0%
AACAGAACAACCTCAGTCGC+AGG - chr6_4:14538640-14538659 None:intergenic 50.0%
!!! ATTGGGTTTTAGGCCCCGTT+TGG + chr6_4:14538935-14538954 Msa0978500:intron 50.0%
GTCGTGTCTGGTGTTTGTGT+CGG + chr6_4:14539344-14539363 Msa0978500:intron 50.0%
GTCGTGTCTGGTGTTTGTGT+CGG + chr6_4:14539344-14539363 Msa0978500:intron 50.0%
ACTTTCAGCTCCTGCGACTG+AGG + chr6_4:14538627-14538646 Msa0978500:CDS 55.0%
ACTTTCAGCTCCTGCGACTG+AGG + chr6_4:14538627-14538646 Msa0978500:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 14538551 14539709 14538551 ID=Msa0978500;Name=Msa0978500
chr6_4 mRNA 14538551 14539709 14538551 ID=Msa0978500-mRNA-1;Parent=Msa0978500;Name=Msa0978500-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|1|0.5|1|0|0.5|2|0|113
chr6_4 exon 14538551 14538740 14538551 ID=Msa0978500-mRNA-1:exon:4760;Parent=Msa0978500-mRNA-1
chr6_4 exon 14539558 14539709 14539558 ID=Msa0978500-mRNA-1:exon:4761;Parent=Msa0978500-mRNA-1
chr6_4 CDS 14538551 14538740 14538551 ID=Msa0978500-mRNA-1:cds;Parent=Msa0978500-mRNA-1
chr6_4 CDS 14539558 14539709 14539558 ID=Msa0978500-mRNA-1:cds;Parent=Msa0978500-mRNA-1
Gene Sequence

>Msa0978500

GAAGCTTCTCATTTTACCTCAAAAAAAAAAAAAAACAAAAAAGAAGTTTTCCATTCTTCGGTTTTTGGCTGTTTTTACTTTCAGCTCCTGCGACTGAGGTTGTTCTGTTCTTCTGAATCCTCAAAGGTTGAAATGGGTGGTTACATTCAAGAAGCACGCGAGAATCACGTGAAGAAGAAAGTCGAAGAAGCTTTACGGAGTAAAATGAAGGTGAAGGCACTTAAAGAGTGTGATTTTTACACACAGAAATATGCTGAGTGTGCTATGGGGAGAACACTTTCTGTTGTTTGGCAATGTCGGAAAGTAGCTAAGGGGTTGAATAATTGTCTTCATCAATTGTAA

Protein sequence

>Msa0978500

EASHFTSKKKKNKKEVFHSSVFGCFYFQLLRLRLFCSSESSKVEMGGYIQEARENHVKKKVEEALRSKMKVKALKECDFYTQKYAECAMGRTLSVVWQCRKVAKGLNNCLHQL*