AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0164980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGAAACGTGAAGGCTTT+TGG 0.238701 1_4:+44922433 Msa0164980:CDS
TGGAAAATCCATCCAAGTTT+TGG 0.260624 1_4:+44922391 Msa0164980:CDS
CTAAGTTTGCCTAGAGCTAT+TGG 0.284593 1_4:-44922913 None:intergenic
TTGAGAATACGGTTAAATTC+TGG 0.315463 1_4:+44922371 Msa0164980:CDS
GGTCGAAAACATGGTTGAAA+TGG 0.329615 1_4:-44921439 None:intergenic
GCTGAAGCGGTTTCCATCAT+TGG 0.338403 1_4:+44922340 Msa0164980:CDS
CGTCTGGCCTACTGCCTAAA+TGG 0.359726 1_4:+44922463 Msa0164980:CDS
GAAATCTCCAAAATGACAAT+AGG 0.421681 1_4:+44922870 Msa0164980:three_prime_UTR
AAATATCCGTCACCAAAACT+TGG 0.426178 1_4:-44922403 None:intergenic
GGAAGAGCAATAAGAAATGA+AGG 0.443857 1_4:+44922651 Msa0164980:CDS
CTCAACTTTACATCCAATGA+TGG 0.454499 1_4:-44922353 None:intergenic
GAAAGCAGATAAATCAAAGA+AGG 0.460322 1_4:+44922601 Msa0164980:CDS
TGGATGTAAAGTTGAGAATA+CGG 0.468666 1_4:+44922360 Msa0164980:CDS
AAAATGACAATAGGTTGAAA+AGG 0.473813 1_4:+44922879 Msa0164980:three_prime_UTR
GTCGAAAACATGGTTGAAAT+GGG 0.474047 1_4:-44921438 None:intergenic
ATGATGGAAACCGCTTCAGC+TGG 0.521428 1_4:-44922337 None:intergenic
TTCTTCTTCTCCTTCGTCTG+AGG 0.523655 1_4:-44921399 None:intergenic
TTAATTTATGATCGAAGAAG+TGG 0.529345 1_4:+44922676 Msa0164980:three_prime_UTR
TTATTCACCATTTAGGCAGT+AGG 0.530913 1_4:-44922470 None:intergenic
ATCCGTCACCAAAACTTGGA+TGG 0.541453 1_4:-44922399 None:intergenic
GAAAATAGTTAGAAACGTGA+AGG 0.545491 1_4:+44922426 Msa0164980:CDS
CATGTGTGTCCAATAGCTCT+AGG 0.550624 1_4:+44922904 Msa0164980:three_prime_UTR
ATTTATGATCGAAGAAGTGG+AGG 0.572124 1_4:+44922679 Msa0164980:three_prime_UTR
ATGGTATGCACCTCAGACGA+AGG 0.594976 1_4:+44921389 Msa0164980:CDS
AAAGCAGATAAATCAAAGAA+GGG 0.598620 1_4:+44922602 Msa0164980:CDS
AAAATGCAAGGAAAGGCAGA+GGG 0.643041 1_4:+44922630 Msa0164980:CDS
GCAAACTTAGAAATTACAAG+AGG 0.646201 1_4:+44922926 Msa0164980:three_prime_UTR
TTTATGATCGAAGAAGTGGA+GGG 0.648481 1_4:+44922680 Msa0164980:three_prime_UTR
GCAGATAAATCAAAGAAGGG+AGG 0.664030 1_4:+44922605 Msa0164980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTTTAATAATAACAT+AGG + chr1_4:44921553-44921572 Msa0164980:intron 10.0%
!!! TTCTTTTTTAATAATAACAT+AGG + chr1_4:44921553-44921572 Msa0164980:intron 10.0%
!! ATATTTAATCAAGGAAATTA+CGG - chr1_4:44921463-44921482 None:intergenic 15.0%
!! TAATTTCCTTGATTAAATAT+AGG + chr1_4:44921463-44921482 Msa0164980:intron 15.0%
!!! ATTCTTTTGTAATATGTTAT+TGG - chr1_4:44921857-44921876 None:intergenic 15.0%
!! ATATTTAATCAAGGAAATTA+CGG - chr1_4:44921463-44921482 None:intergenic 15.0%
!! TAATTTCCTTGATTAAATAT+AGG + chr1_4:44921463-44921482 Msa0164980:intron 15.0%
!!! ATTCTTTTGTAATATGTTAT+TGG - chr1_4:44921857-44921876 None:intergenic 15.0%
!! TTGTTACCTATATTTAATCA+AGG - chr1_4:44921472-44921491 None:intergenic 20.0%
!!! ACAACTTTTTGAAACTTAAA+GGG - chr1_4:44921596-44921615 None:intergenic 20.0%
!!! TTTTCAAAATGTTACGATTA+TGG - chr1_4:44921683-44921702 None:intergenic 20.0%
!!! GTAACATTTTGAAAAGATAA+GGG + chr1_4:44921688-44921707 Msa0164980:intron 20.0%
!!! TAACATTTTGAAAAGATAAG+GGG + chr1_4:44921689-44921708 Msa0164980:intron 20.0%
!! TTCATATTTCACTTATGTTA+GGG + chr1_4:44921719-44921738 Msa0164980:intron 20.0%
!!! TTCTGTCATTTTTTTTTCAT+AGG + chr1_4:44921779-44921798 Msa0164980:intron 20.0%
!!! TCTGTCATTTTTTTTTCATA+GGG + chr1_4:44921780-44921799 Msa0164980:intron 20.0%
!! CAAATAGGTAAAAGAAAAAA+GGG - chr1_4:44921911-44921930 None:intergenic 20.0%
!! ACAAATAGGTAAAAGAAAAA+AGG - chr1_4:44921912-44921931 None:intergenic 20.0%
!! ATGATTATTTACCTAAACTA+AGG - chr1_4:44921989-44922008 None:intergenic 20.0%
!! TGTTACTCTAGATATAAATT+AGG + chr1_4:44922120-44922139 Msa0164980:intron 20.0%
!! GTTACTCTAGATATAAATTA+GGG + chr1_4:44922121-44922140 Msa0164980:intron 20.0%
!! TCAAAAACTTAACTATCAAA+CGG - chr1_4:44922295-44922314 None:intergenic 20.0%
!!! ATAACTTTTATTCACCATTT+AGG - chr1_4:44922480-44922499 None:intergenic 20.0%
!! TTGTTACCTATATTTAATCA+AGG - chr1_4:44921472-44921491 None:intergenic 20.0%
!!! ACAACTTTTTGAAACTTAAA+GGG - chr1_4:44921596-44921615 None:intergenic 20.0%
!!! TTTTCAAAATGTTACGATTA+TGG - chr1_4:44921683-44921702 None:intergenic 20.0%
!!! GTAACATTTTGAAAAGATAA+GGG + chr1_4:44921688-44921707 Msa0164980:intron 20.0%
!!! TAACATTTTGAAAAGATAAG+GGG + chr1_4:44921689-44921708 Msa0164980:intron 20.0%
!! TTCATATTTCACTTATGTTA+GGG + chr1_4:44921719-44921738 Msa0164980:intron 20.0%
!!! TTCTGTCATTTTTTTTTCAT+AGG + chr1_4:44921779-44921798 Msa0164980:intron 20.0%
!!! TCTGTCATTTTTTTTTCATA+GGG + chr1_4:44921780-44921799 Msa0164980:intron 20.0%
!! CAAATAGGTAAAAGAAAAAA+GGG - chr1_4:44921911-44921930 None:intergenic 20.0%
!! ACAAATAGGTAAAAGAAAAA+AGG - chr1_4:44921912-44921931 None:intergenic 20.0%
!! ATGATTATTTACCTAAACTA+AGG - chr1_4:44921989-44922008 None:intergenic 20.0%
!! TGTTACTCTAGATATAAATT+AGG + chr1_4:44922120-44922139 Msa0164980:intron 20.0%
!! GTTACTCTAGATATAAATTA+GGG + chr1_4:44922121-44922140 Msa0164980:intron 20.0%
!! TCAAAAACTTAACTATCAAA+CGG - chr1_4:44922295-44922314 None:intergenic 20.0%
!!! ATAACTTTTATTCACCATTT+AGG - chr1_4:44922480-44922499 None:intergenic 20.0%
!!! AACTTTTTGAAACTTAAAGG+GGG - chr1_4:44921594-44921613 None:intergenic 25.0%
!!! CAACTTTTTGAAACTTAAAG+GGG - chr1_4:44921595-44921614 None:intergenic 25.0%
!!! CACAACTTTTTGAAACTTAA+AGG - chr1_4:44921597-44921616 None:intergenic 25.0%
!! GTTTCAAAAAGTTGTGATTT+TGG + chr1_4:44921601-44921620 Msa0164980:intron 25.0%
!!! CGTAACATTTTGAAAAGATA+AGG + chr1_4:44921687-44921706 Msa0164980:intron 25.0%
! GTTCATATTTCACTTATGTT+AGG + chr1_4:44921718-44921737 Msa0164980:intron 25.0%
! TCATATTTCACTTATGTTAG+GGG + chr1_4:44921720-44921739 Msa0164980:intron 25.0%
!!! CTGTCATTTTTTTTTCATAG+GGG + chr1_4:44921781-44921800 Msa0164980:intron 25.0%
!!! TGTCATTTTTTTTTCATAGG+GGG + chr1_4:44921782-44921801 Msa0164980:intron 25.0%
! TAAATCAACCAACATGTATT+AGG - chr1_4:44922544-44922563 None:intergenic 25.0%
! AAAGCAGATAAATCAAAGAA+GGG + chr1_4:44922602-44922621 Msa0164980:CDS 25.0%
! TTAATTTATGATCGAAGAAG+TGG + chr1_4:44922676-44922695 Msa0164980:three_prime_UTR 25.0%
! GTAAAAAGTAAATAATGCAC+AGG + chr1_4:44922791-44922810 Msa0164980:three_prime_UTR 25.0%
!!! TTTTCAACCTATTGTCATTT+TGG - chr1_4:44922880-44922899 None:intergenic 25.0%
!! AAAATGACAATAGGTTGAAA+AGG + chr1_4:44922879-44922898 Msa0164980:three_prime_UTR 25.0%
!!! AACTTTTTGAAACTTAAAGG+GGG - chr1_4:44921594-44921613 None:intergenic 25.0%
!!! CAACTTTTTGAAACTTAAAG+GGG - chr1_4:44921595-44921614 None:intergenic 25.0%
!!! CACAACTTTTTGAAACTTAA+AGG - chr1_4:44921597-44921616 None:intergenic 25.0%
!! GTTTCAAAAAGTTGTGATTT+TGG + chr1_4:44921601-44921620 Msa0164980:intron 25.0%
!!! CGTAACATTTTGAAAAGATA+AGG + chr1_4:44921687-44921706 Msa0164980:intron 25.0%
! GTTCATATTTCACTTATGTT+AGG + chr1_4:44921718-44921737 Msa0164980:intron 25.0%
! TCATATTTCACTTATGTTAG+GGG + chr1_4:44921720-44921739 Msa0164980:intron 25.0%
!!! CTGTCATTTTTTTTTCATAG+GGG + chr1_4:44921781-44921800 Msa0164980:intron 25.0%
!!! TGTCATTTTTTTTTCATAGG+GGG + chr1_4:44921782-44921801 Msa0164980:intron 25.0%
! TAAATCAACCAACATGTATT+AGG - chr1_4:44922544-44922563 None:intergenic 25.0%
! AAAGCAGATAAATCAAAGAA+GGG + chr1_4:44922602-44922621 Msa0164980:CDS 25.0%
! TTAATTTATGATCGAAGAAG+TGG + chr1_4:44922676-44922695 Msa0164980:three_prime_UTR 25.0%
! GTAAAAAGTAAATAATGCAC+AGG + chr1_4:44922791-44922810 Msa0164980:three_prime_UTR 25.0%
!!! TTTTCAACCTATTGTCATTT+TGG - chr1_4:44922880-44922899 None:intergenic 25.0%
!! AAAATGACAATAGGTTGAAA+AGG + chr1_4:44922879-44922898 Msa0164980:three_prime_UTR 25.0%
ATCAAACAAATCAAGAAGCT+TGG - chr1_4:44921510-44921529 None:intergenic 30.0%
CATATTTCACTTATGTTAGG+GGG + chr1_4:44921721-44921740 Msa0164980:intron 30.0%
!!! TCGCAACTTTTTGAAACTTA+AGG + chr1_4:44921811-44921830 Msa0164980:intron 30.0%
!!! CGCAACTTTTTGAAACTTAA+GGG + chr1_4:44921812-44921831 Msa0164980:intron 30.0%
!!! GCAACTTTTTGAAACTTAAG+GGG + chr1_4:44921813-44921832 Msa0164980:intron 30.0%
!!! CAACTTTTTGAAACTTAAGG+GGG + chr1_4:44921814-44921833 Msa0164980:intron 30.0%
TAGGTAAAAGAAAAAAGGGA+AGG - chr1_4:44921907-44921926 None:intergenic 30.0%
TTACCTATTTGTAATCACAG+TGG + chr1_4:44921920-44921939 Msa0164980:intron 30.0%
TTACCACTGTGATTACAAAT+AGG - chr1_4:44921926-44921945 None:intergenic 30.0%
! TAACTTTTACAAAATCACCC+TGG + chr1_4:44921958-44921977 Msa0164980:intron 30.0%
TGGATGTAAAGTTGAGAATA+CGG + chr1_4:44922360-44922379 Msa0164980:CDS 30.0%
TTGAGAATACGGTTAAATTC+TGG + chr1_4:44922371-44922390 Msa0164980:CDS 30.0%
GAAAATAGTTAGAAACGTGA+AGG + chr1_4:44922426-44922445 Msa0164980:CDS 30.0%
TGTTTCAACCTAATACATGT+TGG + chr1_4:44922533-44922552 Msa0164980:intron 30.0%
! ATACATGTTGGTTGATTTAC+AGG + chr1_4:44922545-44922564 Msa0164980:intron 30.0%
! TACATGTTGGTTGATTTACA+GGG + chr1_4:44922546-44922565 Msa0164980:intron 30.0%
! AACACGTATTGTATTTTGCA+AGG + chr1_4:44922571-44922590 Msa0164980:CDS 30.0%
! ACACGTATTGTATTTTGCAA+GGG + chr1_4:44922572-44922591 Msa0164980:CDS 30.0%
GAAAGCAGATAAATCAAAGA+AGG + chr1_4:44922601-44922620 Msa0164980:CDS 30.0%
!!! ACCTCTTTTTTATATGGACA+CGG - chr1_4:44922743-44922762 None:intergenic 30.0%
!!! GTGTAGACCTCTTTTTTATA+TGG - chr1_4:44922749-44922768 None:intergenic 30.0%
GAAATCTCCAAAATGACAAT+AGG + chr1_4:44922870-44922889 Msa0164980:three_prime_UTR 30.0%
GCAAACTTAGAAATTACAAG+AGG + chr1_4:44922926-44922945 Msa0164980:three_prime_UTR 30.0%
ATCAAACAAATCAAGAAGCT+TGG - chr1_4:44921510-44921529 None:intergenic 30.0%
CATATTTCACTTATGTTAGG+GGG + chr1_4:44921721-44921740 Msa0164980:intron 30.0%
!!! TCGCAACTTTTTGAAACTTA+AGG + chr1_4:44921811-44921830 Msa0164980:intron 30.0%
!!! CGCAACTTTTTGAAACTTAA+GGG + chr1_4:44921812-44921831 Msa0164980:intron 30.0%
!!! GCAACTTTTTGAAACTTAAG+GGG + chr1_4:44921813-44921832 Msa0164980:intron 30.0%
!!! CAACTTTTTGAAACTTAAGG+GGG + chr1_4:44921814-44921833 Msa0164980:intron 30.0%
TAGGTAAAAGAAAAAAGGGA+AGG - chr1_4:44921907-44921926 None:intergenic 30.0%
TTACCTATTTGTAATCACAG+TGG + chr1_4:44921920-44921939 Msa0164980:intron 30.0%
TTACCACTGTGATTACAAAT+AGG - chr1_4:44921926-44921945 None:intergenic 30.0%
! TAACTTTTACAAAATCACCC+TGG + chr1_4:44921958-44921977 Msa0164980:intron 30.0%
TGGATGTAAAGTTGAGAATA+CGG + chr1_4:44922360-44922379 Msa0164980:CDS 30.0%
TTGAGAATACGGTTAAATTC+TGG + chr1_4:44922371-44922390 Msa0164980:CDS 30.0%
GAAAATAGTTAGAAACGTGA+AGG + chr1_4:44922426-44922445 Msa0164980:CDS 30.0%
TGTTTCAACCTAATACATGT+TGG + chr1_4:44922533-44922552 Msa0164980:intron 30.0%
! ATACATGTTGGTTGATTTAC+AGG + chr1_4:44922545-44922564 Msa0164980:intron 30.0%
! TACATGTTGGTTGATTTACA+GGG + chr1_4:44922546-44922565 Msa0164980:intron 30.0%
! AACACGTATTGTATTTTGCA+AGG + chr1_4:44922571-44922590 Msa0164980:CDS 30.0%
! ACACGTATTGTATTTTGCAA+GGG + chr1_4:44922572-44922591 Msa0164980:CDS 30.0%
GAAAGCAGATAAATCAAAGA+AGG + chr1_4:44922601-44922620 Msa0164980:CDS 30.0%
!!! ACCTCTTTTTTATATGGACA+CGG - chr1_4:44922743-44922762 None:intergenic 30.0%
!!! GTGTAGACCTCTTTTTTATA+TGG - chr1_4:44922749-44922768 None:intergenic 30.0%
GAAATCTCCAAAATGACAAT+AGG + chr1_4:44922870-44922889 Msa0164980:three_prime_UTR 30.0%
GCAAACTTAGAAATTACAAG+AGG + chr1_4:44922926-44922945 Msa0164980:three_prime_UTR 30.0%
AAGGAGAAGAAGAAGAAGAT+GGG + chr1_4:44921408-44921427 Msa0164980:CDS 35.0%
GTCGAAAACATGGTTGAAAT+GGG - chr1_4:44921441-44921460 None:intergenic 35.0%
!!! TCAGTAATTTTGGGACGAAA+GGG - chr1_4:44921632-44921651 None:intergenic 35.0%
AGAGCATATTTCCCTAAACA+TGG + chr1_4:44921749-44921768 Msa0164980:intron 35.0%
GAGCATATTTCCCTAAACAT+GGG + chr1_4:44921750-44921769 Msa0164980:intron 35.0%
AGCATATTTCCCTAAACATG+GGG + chr1_4:44921751-44921770 Msa0164980:intron 35.0%
GCGTTGTGGAGAAATAAAAT+TGG + chr1_4:44922047-44922066 Msa0164980:intron 35.0%
GACTATAACTATGCAACTTG+TGG - chr1_4:44922088-44922107 None:intergenic 35.0%
! ATTTTTATAGCCAGCTGAAG+CGG + chr1_4:44922327-44922346 Msa0164980:intron 35.0%
CTCAACTTTACATCCAATGA+TGG - chr1_4:44922356-44922375 None:intergenic 35.0%
! TGGAAAATCCATCCAAGTTT+TGG + chr1_4:44922391-44922410 Msa0164980:CDS 35.0%
AAATATCCGTCACCAAAACT+TGG - chr1_4:44922406-44922425 None:intergenic 35.0%
! TTAGAAACGTGAAGGCTTTT+GGG + chr1_4:44922434-44922453 Msa0164980:CDS 35.0%
TTATTCACCATTTAGGCAGT+AGG - chr1_4:44922473-44922492 None:intergenic 35.0%
GGAAGAGCAATAAGAAATGA+AGG + chr1_4:44922651-44922670 Msa0164980:CDS 35.0%
ATTTATGATCGAAGAAGTGG+AGG + chr1_4:44922679-44922698 Msa0164980:three_prime_UTR 35.0%
TTTATGATCGAAGAAGTGGA+GGG + chr1_4:44922680-44922699 Msa0164980:three_prime_UTR 35.0%
!!! CCTCTTTTTTATATGGACAC+GGG - chr1_4:44922742-44922761 None:intergenic 35.0%
AAGGAGAAGAAGAAGAAGAT+GGG + chr1_4:44921408-44921427 Msa0164980:CDS 35.0%
GTCGAAAACATGGTTGAAAT+GGG - chr1_4:44921441-44921460 None:intergenic 35.0%
!!! TCAGTAATTTTGGGACGAAA+GGG - chr1_4:44921632-44921651 None:intergenic 35.0%
AGAGCATATTTCCCTAAACA+TGG + chr1_4:44921749-44921768 Msa0164980:intron 35.0%
GAGCATATTTCCCTAAACAT+GGG + chr1_4:44921750-44921769 Msa0164980:intron 35.0%
AGCATATTTCCCTAAACATG+GGG + chr1_4:44921751-44921770 Msa0164980:intron 35.0%
GCGTTGTGGAGAAATAAAAT+TGG + chr1_4:44922047-44922066 Msa0164980:intron 35.0%
GACTATAACTATGCAACTTG+TGG - chr1_4:44922088-44922107 None:intergenic 35.0%
! ATTTTTATAGCCAGCTGAAG+CGG + chr1_4:44922327-44922346 Msa0164980:intron 35.0%
CTCAACTTTACATCCAATGA+TGG - chr1_4:44922356-44922375 None:intergenic 35.0%
! TGGAAAATCCATCCAAGTTT+TGG + chr1_4:44922391-44922410 Msa0164980:CDS 35.0%
AAATATCCGTCACCAAAACT+TGG - chr1_4:44922406-44922425 None:intergenic 35.0%
! TTAGAAACGTGAAGGCTTTT+GGG + chr1_4:44922434-44922453 Msa0164980:CDS 35.0%
TTATTCACCATTTAGGCAGT+AGG - chr1_4:44922473-44922492 None:intergenic 35.0%
GGAAGAGCAATAAGAAATGA+AGG + chr1_4:44922651-44922670 Msa0164980:CDS 35.0%
ATTTATGATCGAAGAAGTGG+AGG + chr1_4:44922679-44922698 Msa0164980:three_prime_UTR 35.0%
TTTATGATCGAAGAAGTGGA+GGG + chr1_4:44922680-44922699 Msa0164980:three_prime_UTR 35.0%
!!! CCTCTTTTTTATATGGACAC+GGG - chr1_4:44922742-44922761 None:intergenic 35.0%
GAAGGAGAAGAAGAAGAAGA+TGG + chr1_4:44921407-44921426 Msa0164980:CDS 40.0%
GGTCGAAAACATGGTTGAAA+TGG - chr1_4:44921442-44921461 None:intergenic 40.0%
GGAAATTACGGTCGAAAACA+TGG - chr1_4:44921451-44921470 None:intergenic 40.0%
!!! TAATTTTGGGACGAAAGGGA+CGG - chr1_4:44921628-44921647 None:intergenic 40.0%
!!! CTCAGTAATTTTGGGACGAA+AGG - chr1_4:44921633-44921652 None:intergenic 40.0%
! GGCGTCTTCTCAGTAATTTT+GGG - chr1_4:44921641-44921660 None:intergenic 40.0%
! TGGCGTCTTCTCAGTAATTT+TGG - chr1_4:44921642-44921661 None:intergenic 40.0%
!!! ACGATTATGGCCCCTTTTTT+GGG - chr1_4:44921670-44921689 None:intergenic 40.0%
!!! TACGATTATGGCCCCTTTTT+TGG - chr1_4:44921671-44921690 None:intergenic 40.0%
GCATATTTCCCTAAACATGG+GGG + chr1_4:44921752-44921771 Msa0164980:intron 40.0%
! AGGGCTCTATGAATCACTTT+TGG + chr1_4:44922140-44922159 Msa0164980:intron 40.0%
!! ATCCATCCAAGTTTTGGTGA+CGG + chr1_4:44922397-44922416 Msa0164980:CDS 40.0%
! GTTAGAAACGTGAAGGCTTT+TGG + chr1_4:44922433-44922452 Msa0164980:CDS 40.0%
GCAGATAAATCAAAGAAGGG+AGG + chr1_4:44922605-44922624 Msa0164980:CDS 40.0%
AGAAGGGAGGAAAAAATGCA+AGG + chr1_4:44922618-44922637 Msa0164980:CDS 40.0%
GGAGGAAAAAATGCAAGGAA+AGG + chr1_4:44922623-44922642 Msa0164980:CDS 40.0%
AAAAATGCAAGGAAAGGCAG+AGG + chr1_4:44922629-44922648 Msa0164980:CDS 40.0%
AAAATGCAAGGAAAGGCAGA+GGG + chr1_4:44922630-44922649 Msa0164980:CDS 40.0%
CCCGTGTCCATATAAAAAAG+AGG + chr1_4:44922739-44922758 Msa0164980:three_prime_UTR 40.0%
CTAAGTTTGCCTAGAGCTAT+TGG - chr1_4:44922916-44922935 None:intergenic 40.0%
GAAGGAGAAGAAGAAGAAGA+TGG + chr1_4:44921407-44921426 Msa0164980:CDS 40.0%
GGTCGAAAACATGGTTGAAA+TGG - chr1_4:44921442-44921461 None:intergenic 40.0%
GGAAATTACGGTCGAAAACA+TGG - chr1_4:44921451-44921470 None:intergenic 40.0%
!!! TAATTTTGGGACGAAAGGGA+CGG - chr1_4:44921628-44921647 None:intergenic 40.0%
!!! CTCAGTAATTTTGGGACGAA+AGG - chr1_4:44921633-44921652 None:intergenic 40.0%
! GGCGTCTTCTCAGTAATTTT+GGG - chr1_4:44921641-44921660 None:intergenic 40.0%
! TGGCGTCTTCTCAGTAATTT+TGG - chr1_4:44921642-44921661 None:intergenic 40.0%
!!! ACGATTATGGCCCCTTTTTT+GGG - chr1_4:44921670-44921689 None:intergenic 40.0%
!!! TACGATTATGGCCCCTTTTT+TGG - chr1_4:44921671-44921690 None:intergenic 40.0%
GCATATTTCCCTAAACATGG+GGG + chr1_4:44921752-44921771 Msa0164980:intron 40.0%
! AGGGCTCTATGAATCACTTT+TGG + chr1_4:44922140-44922159 Msa0164980:intron 40.0%
!! ATCCATCCAAGTTTTGGTGA+CGG + chr1_4:44922397-44922416 Msa0164980:CDS 40.0%
! GTTAGAAACGTGAAGGCTTT+TGG + chr1_4:44922433-44922452 Msa0164980:CDS 40.0%
GCAGATAAATCAAAGAAGGG+AGG + chr1_4:44922605-44922624 Msa0164980:CDS 40.0%
AGAAGGGAGGAAAAAATGCA+AGG + chr1_4:44922618-44922637 Msa0164980:CDS 40.0%
GGAGGAAAAAATGCAAGGAA+AGG + chr1_4:44922623-44922642 Msa0164980:CDS 40.0%
AAAAATGCAAGGAAAGGCAG+AGG + chr1_4:44922629-44922648 Msa0164980:CDS 40.0%
AAAATGCAAGGAAAGGCAGA+GGG + chr1_4:44922630-44922649 Msa0164980:CDS 40.0%
CCCGTGTCCATATAAAAAAG+AGG + chr1_4:44922739-44922758 Msa0164980:three_prime_UTR 40.0%
CTAAGTTTGCCTAGAGCTAT+TGG - chr1_4:44922916-44922935 None:intergenic 40.0%
TTCTTCTTCTCCTTCGTCTG+AGG - chr1_4:44921402-44921421 None:intergenic 45.0%
!!! AATTTTGGGACGAAAGGGAC+GGG - chr1_4:44921627-44921646 None:intergenic 45.0%
ACGCCACATGTCCCAAAAAA+GGG + chr1_4:44921656-44921675 Msa0164980:intron 45.0%
AGAAGTGACCCCCATGTTTA+GGG - chr1_4:44921763-44921782 None:intergenic 45.0%
CCTAAACTAAGGATGAGCCA+GGG - chr1_4:44921978-44921997 None:intergenic 45.0%
ACCTAAACTAAGGATGAGCC+AGG - chr1_4:44921979-44921998 None:intergenic 45.0%
AACTGCAACTGTTTGCGTTG+TGG + chr1_4:44922033-44922052 Msa0164980:intron 45.0%
ATCCGTCACCAAAACTTGGA+TGG - chr1_4:44922402-44922421 None:intergenic 45.0%
!!! AACGTGAAGGCTTTTGGGAT+TGG + chr1_4:44922439-44922458 Msa0164980:CDS 45.0%
!!! ACGTGAAGGCTTTTGGGATT+GGG + chr1_4:44922440-44922459 Msa0164980:CDS 45.0%
CATGTGTGTCCAATAGCTCT+AGG + chr1_4:44922904-44922923 Msa0164980:three_prime_UTR 45.0%
TTCTTCTTCTCCTTCGTCTG+AGG - chr1_4:44921402-44921421 None:intergenic 45.0%
!!! AATTTTGGGACGAAAGGGAC+GGG - chr1_4:44921627-44921646 None:intergenic 45.0%
ACGCCACATGTCCCAAAAAA+GGG + chr1_4:44921656-44921675 Msa0164980:intron 45.0%
AGAAGTGACCCCCATGTTTA+GGG - chr1_4:44921763-44921782 None:intergenic 45.0%
CCTAAACTAAGGATGAGCCA+GGG - chr1_4:44921978-44921997 None:intergenic 45.0%
ACCTAAACTAAGGATGAGCC+AGG - chr1_4:44921979-44921998 None:intergenic 45.0%
AACTGCAACTGTTTGCGTTG+TGG + chr1_4:44922033-44922052 Msa0164980:intron 45.0%
ATCCGTCACCAAAACTTGGA+TGG - chr1_4:44922402-44922421 None:intergenic 45.0%
!!! AACGTGAAGGCTTTTGGGAT+TGG + chr1_4:44922439-44922458 Msa0164980:CDS 45.0%
!!! ACGTGAAGGCTTTTGGGATT+GGG + chr1_4:44922440-44922459 Msa0164980:CDS 45.0%
CATGTGTGTCCAATAGCTCT+AGG + chr1_4:44922904-44922923 Msa0164980:three_prime_UTR 45.0%
ATGGTATGCACCTCAGACGA+AGG + chr1_4:44921389-44921408 Msa0164980:CDS 50.0%
GACGCCACATGTCCCAAAAA+AGG + chr1_4:44921655-44921674 Msa0164980:intron 50.0%
CGCCACATGTCCCAAAAAAG+GGG + chr1_4:44921657-44921676 Msa0164980:intron 50.0%
CAGAAGTGACCCCCATGTTT+AGG - chr1_4:44921764-44921783 None:intergenic 50.0%
CCCTGGCTCATCCTTAGTTT+AGG + chr1_4:44921975-44921994 Msa0164980:intron 50.0%
! ATGATGGAAACCGCTTCAGC+TGG - chr1_4:44922340-44922359 None:intergenic 50.0%
GCTGAAGCGGTTTCCATCAT+TGG + chr1_4:44922340-44922359 Msa0164980:CDS 50.0%
ATGGTATGCACCTCAGACGA+AGG + chr1_4:44921389-44921408 Msa0164980:CDS 50.0%
GACGCCACATGTCCCAAAAA+AGG + chr1_4:44921655-44921674 Msa0164980:intron 50.0%
CGCCACATGTCCCAAAAAAG+GGG + chr1_4:44921657-44921676 Msa0164980:intron 50.0%
CAGAAGTGACCCCCATGTTT+AGG - chr1_4:44921764-44921783 None:intergenic 50.0%
CCCTGGCTCATCCTTAGTTT+AGG + chr1_4:44921975-44921994 Msa0164980:intron 50.0%
! ATGATGGAAACCGCTTCAGC+TGG - chr1_4:44922340-44922359 None:intergenic 50.0%
GCTGAAGCGGTTTCCATCAT+TGG + chr1_4:44922340-44922359 Msa0164980:CDS 50.0%
!!! GGCCCCTTTTTTGGGACATG+TGG - chr1_4:44921662-44921681 None:intergenic 55.0%
CGTCTGGCCTACTGCCTAAA+TGG + chr1_4:44922463-44922482 Msa0164980:CDS 55.0%
!!! GGCCCCTTTTTTGGGACATG+TGG - chr1_4:44921662-44921681 None:intergenic 55.0%
CGTCTGGCCTACTGCCTAAA+TGG + chr1_4:44922463-44922482 Msa0164980:CDS 55.0%
!!! GGCTTTTGGGATTGGGCGTC+TGG + chr1_4:44922447-44922466 Msa0164980:CDS 60.0%
!!! GGCTTTTGGGATTGGGCGTC+TGG + chr1_4:44922447-44922466 Msa0164980:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 44921389 44922971 44921389 ID=Msa0164980;Name=Msa0164980
chr1_4 mRNA 44921389 44922971 44921389 ID=Msa0164980-mRNA-1;Parent=Msa0164980;Name=Msa0164980-mRNA-1;_AED=0.08;_eAED=0.17;_QI=0|0.5|0.33|1|1|1|3|301|104
chr1_4 exon 44921389 44921451 44921389 ID=Msa0164980-mRNA-1:exon:12910;Parent=Msa0164980-mRNA-1
chr1_4 exon 44922337 44922484 44922337 ID=Msa0164980-mRNA-1:exon:12911;Parent=Msa0164980-mRNA-1
chr1_4 exon 44922567 44922971 44922567 ID=Msa0164980-mRNA-1:exon:12912;Parent=Msa0164980-mRNA-1
chr1_4 CDS 44921389 44921451 44921389 ID=Msa0164980-mRNA-1:cds;Parent=Msa0164980-mRNA-1
chr1_4 CDS 44922337 44922484 44922337 ID=Msa0164980-mRNA-1:cds;Parent=Msa0164980-mRNA-1
chr1_4 CDS 44922567 44922670 44922567 ID=Msa0164980-mRNA-1:cds;Parent=Msa0164980-mRNA-1
chr1_4 three_prime_UTR 44922671 44922971 44922671 ID=Msa0164980-mRNA-1:three_prime_utr;Parent=Msa0164980-mRNA-1
Gene Sequence

>Msa0164980

ATGGTATGCACCTCAGACGAAGGAGAAGAAGAAGAAGATGGGATGCACTCCCATTTCAACCATCCAGCTGAAGCGGTTTCCATCATTGGATGTAAAGTTGAGAATACGGTTAAATTCTGGAAAATCCATCCAAGTTTTGGTGACGGATATTTGAAAATAGTTAGAAACGTGAAGGCTTTTGGGATTGGGCGTCTGGCCTACTGCCTAAATGGGAAAACACGTATTGTATTTTGCAAGGGAATGAAGAAAGCAGATAAATCAAAGAAGGGAGGAAAAAATGCAAGGAAAGGCAGAGGGAAGAGCAATAAGAAATGA
Protein sequence

>Msa0164980

MVCTSDEGEEEEDGMHSHFNHPAEAVSIIGCKVENTVKFWKIHPSFGDGYLKIVRNVKAFGIGRLAYCLNGKTRIVFCKGMKKADKSKKGGKNARKGRGKSNKK*