AlfalfaGEDB | Msa1006390

M. sativa cultivar zhongmu-4 / Msa1006390

KEGG: K04733
GO: -
PFAM: PF12799.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1006390	AES77384.1	70.439	751	172	6	1	701	41	791	0.0	1008

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1006390	sp\|Q8VZG8\|MIK2_ARATH	40.520	654	338	17	47	669	412	1045	4.90e-145	452
Msa1006390	sp\|Q8VZG8\|MIK2_ARATH	36.940	268	146	4	56	301	160	426	1.13e-38	157
Msa1006390	sp\|Q8VZG8\|MIK2_ARATH	34.164	281	156	5	43	301	129	402	5.24e-31	134
Msa1006390	sp\|Q8VZG8\|MIK2_ARATH	30.982	326	180	9	2	302	50	355	5.94e-27	121

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1006390	G7KZX2	70.439	751	172	6	1	701	41	791	0.0	1008

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0153200	Msa1006390	0.814746	1.316603e-51	-8.615850e-47
Msa0533430	Msa1006390	0.838071	3.575646e-57	-8.615850e-47
Msa0580610	Msa1006390	0.800679	1.287168e-48	-8.615850e-47
Msa0629880	Msa1006390	0.813711	2.229374e-51	-8.615850e-47
Msa0818010	Msa1006390	0.837671	4.529899e-57	-8.615850e-47
Msa0818030	Msa1006390	0.801019	1.096737e-48	-8.615850e-47
Msa0858360	Msa1006390	0.816305	5.921581e-52	-8.615850e-47
Msa0884920	Msa1006390	0.836565	8.686492e-57	-8.615850e-47
Msa0897950	Msa1006390	0.800972	1.121457e-48	-8.615850e-47
Msa0902020	Msa1006390	0.830249	3.266276e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1006390	MtrunA17_Chr7g0217061	70.439	751	172	6	1	701	41	791	1008
Msa1006390	MtrunA17_Chr7g0215011	66.129	744	206	5	1	698	42	785	934
Msa1006390	MtrunA17_Chr7g0218991	65.229	719	227	5	1	700	49	763	921
Msa1006390	MtrunA17_Chr7g0215641	63.770	748	223	6	1	700	27	774	918
Msa1006390	MtrunA17_Chr7g0215671	65.972	720	220	7	1	700	42	756	914
Msa1006390	MtrunA17_Chr7g0215651	64.355	721	229	7	1	700	27	740	900
Msa1006390	MtrunA17_Chr7g0215061	59.747	790	228	8	1	700	39	828	893
Msa1006390	MtrunA17_Chr7g0215631	65.475	727	220	7	1	700	27	749	889
Msa1006390	MtrunA17_Chr7g0215681	63.564	752	215	10	1	700	43	787	875
Msa1006390	MtrunA17_Chr7g0217081	55.972	854	210	8	1	700	26	867	859

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1006390	AT4G08850.1	40.520	654	338	17	47	669	412	1045	4.98e-146	452
Msa1006390	AT4G08850.1	36.940	268	146	4	56	301	160	426	1.15e-39	157
Msa1006390	AT4G08850.1	34.164	281	156	5	43	301	129	402	5.33e-32	134
Msa1006390	AT4G08850.1	30.982	326	180	9	2	302	50	355	6.04e-28	121
Msa1006390	AT1G35710.1	40.599	601	300	13	87	669	559	1120	1.21e-134	424
Msa1006390	AT1G35710.1	36.610	295	140	5	55	303	143	436	1.30e-38	154
Msa1006390	AT1G35710.1	34.819	359	150	9	2	303	33	364	3.45e-36	147
Msa1006390	AT1G35710.1	34.836	244	118	5	64	301	298	506	5.41e-27	118
Msa1006390	AT1G35710.1	29.487	312	172	7	34	298	313	623	2.05e-19	94.0
Msa1006390	AT1G35710.1	37.956	137	84	1	63	199	607	742	7.10e-18	89.0

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Find 128 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCTTAATGTTGTTAA+AGG	0.227426	7_1:+5245473	Msa1006390:CDS
AAACACTTCGGAATGAAGAT+TGG	0.478038	7_1:+5246550	Msa1006390:three_prime_UTR
AAAGGCTTAATGTTGTTAAA+GGG	0.327077	7_1:+5245474	Msa1006390:CDS
AAAGTTGTATAATATTTCCA+AGG	0.578595	7_1:-5244743	None:intergenic
AACAAGGCTGGAAGCATAAA+AGG	0.301293	7_1:+5243911	Msa1006390:CDS
AACCTGTGAAATAAACACTT+CGG	0.627917	7_1:+5246538	Msa1006390:exon
AAGAGATTATTCAATGAAAT+AGG	0.350041	7_1:-5244588	None:intergenic
AAGCCTAGTGTTTCTGACTT+TGG	0.420170	7_1:+5245593	Msa1006390:CDS
AAGGCTTAATGTTGTTAAAG+GGG	0.534362	7_1:+5245475	Msa1006390:CDS
AAGGGATTTGACCACTAATG+AGG	0.532239	7_1:-5244716	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTATATTGAATAGTAGATGG+TGG	+	chr7_1:5243828-5243847	Msa1006390:CDS	30.0%
ACATCTCTCATCTTTGTAAT+TGG	+	chr7_1:5243873-5243892	Msa1006390:CDS	30.0%
CATCTCTCATCTTTGTAATT+GGG	+	chr7_1:5243874-5243893	Msa1006390:CDS	30.0%
ATCTCTAACAACTACATTCA+AGG	+	chr7_1:5244235-5244254	Msa1006390:CDS	30.0%
ATCTCTAACAACTACATTCA+AGG	+	chr7_1:5244235-5244254	Msa1006390:CDS	30.0%
TAATTGTGCAAGGTTTGTAA+GGG	-	chr7_1:5244491-5244510	None:intergenic	30.0%
TTAATTGTGCAAGGTTTGTA+AGG	-	chr7_1:5244492-5244511	None:intergenic	30.0%
ATTTCCCACAACTTACTTAT+TGG	+	chr7_1:5244630-5244649	Msa1006390:CDS	30.0%
GTTACCAATAAGTAAGTTGT+GGG	-	chr7_1:5244637-5244656	None:intergenic	30.0%
GAAAATGGAAACAGATTTGA+AGG	-	chr7_1:5244663-5244682	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	5243815	5246656	5243815	ID=Msa1006390;Name=Msa1006390
chr7_1	mRNA	5243815	5246656	5243815	ID=Msa1006390-mRNA-1;Parent=Msa1006390;Name=Msa1006390-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0\|0.5\|0.33\|1\|1\|1\|3\|110\|701
chr7_1	exon	5243815	5244422	5243815	ID=Msa1006390-mRNA-1:exon:1701;Parent=Msa1006390-mRNA-1
chr7_1	exon	5244578	5245689	5244578	ID=Msa1006390-mRNA-1:exon:1702;Parent=Msa1006390-mRNA-1
chr7_1	exon	5246161	5246656	5246161	ID=Msa1006390-mRNA-1:exon:1703;Parent=Msa1006390-mRNA-1
chr7_1	CDS	5243815	5244422	5243815	ID=Msa1006390-mRNA-1:cds;Parent=Msa1006390-mRNA-1
chr7_1	CDS	5244578	5245689	5244578	ID=Msa1006390-mRNA-1:cds;Parent=Msa1006390-mRNA-1
chr7_1	CDS	5246161	5246546	5246161	ID=Msa1006390-mRNA-1:cds;Parent=Msa1006390-mRNA-1
chr7_1	three_prime_UTR	5246547	5246656	5246547	ID=Msa1006390-mRNA-1:three_prime_utr;Parent=Msa1006390-mRNA-1

Gene Sequence

>Msa1006390

ATGGAAGCAAATGCTATATTGAATAGTAGATGGTGGAACACGTCTGATGAATCTTTCAACATCTCTCATCTTTGTAATTGGGATGAGATACATTGCAACAAGGCTGGAAGCATAAAAGGAATCTACAAATATTCTGCAACAATGTCTGAGATTCACTTTGCAACGCCTAACTTGTCTGTTTTCCACAATTTGGAAAGCCTTTTTGTCAATGGAATTGGACTACAAGGGACTATCCCAGAAGAAATTGGTCTTCTCACCAAGCTCACTGACATTGGTCTATCTGATAATTCAATAGAAGGTAAGATACCTCCTTCGATTGGAAACCTTACTCAATTAAAGAAACTATACATCTTTGAAAATAGCATAAAAGGTGAGATACCTCCTTCACTGGGAAATCTCAAACAATTAGAGGATCTAGACATCTCTAACAACTACATTCAAGGTTTCATTCCTTTTGAGTTAGGGTTCTTGAAAAGTCTCACAAAATTAAATCTATCTAACAATAGAATCAAAGGTGAGATACCTTCTTCACTAGGAAATCTCAAACAATTAAAATACCTAGACATCTCTAACAACTACATTCAAGGTTCCATTCCACTTGAGTTAGGTGGGAACATTCCTATTTCATTGAATAATCTCTTTCATTTAGCCATGCTTGACATTTCCCACAACTTACTTATTGGTAACCTTCCTTCAAATCTGTTTCCATTTTCAAGTAAGGAAATACAAATTGACCTCAGTCACAACCTCATTAGTGGTCAAATCCCTTCTGACCTTGGAAATATTATACAACTTTTTTTAAACAATAATAACCTCATTGGAATGGTACCCAAATCTCTTTGTAATATTCATTATTTAGACATCTCATATAATTATTTTAAGGGTCCTATTCCAAAATGTATTGGTCATGATTCAATAATAGGGAACAAGGATGTATGTACCGACATGTCATATAACCAAATCAACTTCCAATCCAACCCTTGCAAGAAAAGCAATAAAGTGCACATGGTTGTCATTGTGCTTCCTATCTTTATTATTCTAATCCTAGCGTTATCACTTCTCATGTGCTTCAAACTTCGTCATTACATTGTTAAGAACAAGCATGCAAATAGAACAATAACAAAGGATGGGGATTTGTTTTGTATATGGAATTATGATGGCCAAATAGCGTATGATGAGATCATTAAAGCAACAGAAGACTTTGACATGAGATATTGCATTGGGACAGGAGCATATGGAAGTGTTTACAAGGCACAAATACCAAGTGGCAAAATTGTTGCCTTAAAAAAACTTCACAGCTATGAAGCAGAGGTGCCAGCTTTTGATGAGAGTTTTAAAAATGAAGTAAGAATATTATCAGAAATAAGGCACAGAAATATTGTGAAGCTTTATGGATTTTGCCTCCACAAAAGAATCATGTTTTTGATATACGAGTACATGGAGAAAGGTAGCTTGTTCTCTGTCTTGTATGATGAAGTTGAAGCAGTTGAATTCAATTGGAGAAAAAGGCTTAATGTTGTTAAAGGGGTAGCATTTGGTCTGTCATATCTTCATCATGATTGCACCCCTCCAATAGTGCATAGAGATGTATCTGCTAGCAATATCTTGCTAAACTCTGAATGGAAGCCTAGTGTTTCTGACTTTGGAACAGCTCGTATACTTCTGTATGATTCGTCCAATCGAACAATAGTTGCTGGAACTGTTGGATATGTAGCTCCTGAACTTGCCTATACCATGGTTATGAGTGAAAAGTGTGATGTATATAGCTTTGGAGTTGTGGCACTGGAAACATTAATGGGAAGACACCCTGGTGATATATTGTCATCACTTCAATTAGCATCAACTCAACGCATGACATTATGTGAAGTATTAGACCAACGTCTCCCGCTTCCAAATAATATGATGGTTTTACTTGACTTAATTCGTGTTGCCGTAGTTGCATTTACTTGCTTAAATCTCAATCCTTCCTCTCGACCAACAATGAAATGTGTGTCACAAAGTTTTTCCACTGATCTTAAACCATTAAGCATTCCTTTGAGTGAAATTTCTGTGCAACAACTCATGAGTCAAGAACTAAAAGCTATATTTCATATCGGGAACCTGTGA

Protein sequence

>Msa1006390

MEANAILNSRWWNTSDESFNISHLCNWDEIHCNKAGSIKGIYKYSATMSEIHFATPNLSVFHNLESLFVNGIGLQGTIPEEIGLLTKLTDIGLSDNSIEGKIPPSIGNLTQLKKLYIFENSIKGEIPPSLGNLKQLEDLDISNNYIQGFIPFELGFLKSLTKLNLSNNRIKGEIPSSLGNLKQLKYLDISNNYIQGSIPLELGGNIPISLNNLFHLAMLDISHNLLIGNLPSNLFPFSSKEIQIDLSHNLISGQIPSDLGNIIQLFLNNNNLIGMVPKSLCNIHYLDISYNYFKGPIPKCIGHDSIIGNKDVCTDMSYNQINFQSNPCKKSNKVHMVVIVLPIFIILILALSLLMCFKLRHYIVKNKHANRTITKDGDLFCIWNYDGQIAYDEIIKATEDFDMRYCIGTGAYGSVYKAQIPSGKIVALKKLHSYEAEVPAFDESFKNEVRILSEIRHRNIVKLYGFCLHKRIMFLIYEYMEKGSLFSVLYDEVEAVEFNWRKRLNVVKGVAFGLSYLHHDCTPPIVHRDVSASNILLNSEWKPSVSDFGTARILLYDSSNRTIVAGTVGYVAPELAYTMVMSEKCDVYSFGVVALETLMGRHPGDILSSLQLASTQRMTLCEVLDQRLPLPNNMMVLLDLIRVAVVAFTCLNLNPSSRPTMKCVSQSFSTDLKPLSIPLSEISVQQLMSQELKAIFHIGNL*