AlfalfaGEDB | Msa0963310

M. sativa cultivar zhongmu-4 / Msa0963310

KEGG: K19307
GO: GO:0000154 GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008649 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016436 GO:0016740 GO:0016741 GO:0022613 GO:0031167 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0070042 GO:0070475 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:0140102 GO:1901360
PFAM: PF10354.12

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0963310	sp\|P0C8L4\|Y4648_ARATH	49.660	147	71	2	10	153	62	208	1.75e-41	140

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0963310	G7KMJ9	69.325	163	48	1	1	161	49	211	1.23e-72	227

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0068630	Msa0963310	0.827702	1.352285e-54	-8.615850e-47
Msa0629850	Msa0963310	0.819513	1.116713e-52	-8.615850e-47
Msa0629880	Msa0963310	0.808266	3.369254e-50	-8.615850e-47
Msa0639460	Msa0963310	0.816502	5.350847e-52	-8.615850e-47
Msa0682360	Msa0963310	0.830830	2.354560e-55	-8.615850e-47
Msa0745110	Msa0963310	0.806815	6.848473e-50	-8.615850e-47
Msa0815320	Msa0963310	0.840751	7.199084e-58	-8.615850e-47
Msa0818010	Msa0963310	0.820741	5.843054e-53	-8.615850e-47
Msa0818030	Msa0963310	0.809582	1.762187e-50	-8.615850e-47
Msa0153200	Msa0963310	0.824760	6.777370e-54	-8.615850e-47
Msa0211680	Msa0963310	0.818684	1.723459e-52	-8.615850e-47
Msa0458700	Msa0963310	0.817103	3.922948e-52	-8.615850e-47
Msa0273570	Msa0963310	0.804731	1.876170e-49	-8.615850e-47
Msa0352000	Msa0963310	0.804377	2.224503e-49	-8.615850e-47
Msa0353690	Msa0963310	0.804021	2.637606e-49	-8.615850e-47
Msa0533290	Msa0963310	0.839999	1.131841e-57	-8.615850e-47
Msa0533430	Msa0963310	0.850143	2.054125e-60	-8.615850e-47
Msa0853550	Msa0963310	0.818193	2.227497e-52	-8.615850e-47
Msa0858360	Msa0963310	0.848534	5.765279e-60	-8.615850e-47
Msa0884920	Msa0963310	0.802992	4.312279e-49	-8.615850e-47
Msa0898360	Msa0963310	0.809742	1.627807e-50	-8.615850e-47
Msa0898390	Msa0963310	0.821220	4.533252e-53	-8.615850e-47
Msa0902020	Msa0963310	0.893489	5.994928e-75	-8.615850e-47
Msa0912370	Msa0963310	0.863400	2.556894e-64	-8.615850e-47
Msa0912500	Msa0963310	0.822626	2.142541e-53	-8.615850e-47
Msa0924370	Msa0963310	0.890115	1.318500e-73	-8.615850e-47
Msa0963310	Msa0964180	0.812612	3.883321e-51	-8.615850e-47
Msa0963310	Msa1006250	0.850707	1.426390e-60	-8.615850e-47
Msa0963310	Msa1006390	0.811848	5.701800e-51	-8.615850e-47
Msa0963310	Msa1027240	0.805892	1.072039e-49	-8.615850e-47
Msa0963310	Msa1131820	0.822766	1.987661e-53	-8.615850e-47
Msa0963310	Msa1187060	0.835853	1.317795e-56	-8.615850e-47
Msa0963310	Msa1232060	0.831976	1.230186e-55	-8.615850e-47
Msa0963310	Msa1288000	0.838613	2.591251e-57	-8.615850e-47
Msa0963310	Msa1349090	0.804550	2.047154e-49	-8.615850e-47
Msa0963310	Msa1351080	0.842144	3.092875e-58	-8.615850e-47
Msa0963310	Msa1359820	0.804798	1.817448e-49	-8.615850e-47
Msa0963310	Msa1409770	0.802795	4.735884e-49	-8.615850e-47
Msa0963310	Msa1423990	0.816440	5.525519e-52	-8.615850e-47
Msa0963310	Msa1444370	0.814135	1.797522e-51	-8.615850e-47
Msa0963310	Msa1463140	0.824548	7.602403e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0963310	MtrunA17_Chr6g0483591	69.325	163	48	1	1	161	49	211	2.37e-76	227
Msa0963310	MtrunA17_Chr6g0483571	62.745	153	54	2	2	151	50	202	1.46e-60	186
Msa0963310	MtrunA17_Chr6g0483551	63.636	154	53	2	2	152	50	203	9.96e-59	182
Msa0963310	MtrunA17_Chr5g0444781	49.068	161	80	1	2	160	54	214	3.66e-52	168
Msa0963310	MtrunA17_Chr3g0103011	48.684	152	76	1	2	151	18	169	4.33e-42	144
Msa0963310	MtrunA17_Chr2g0290801	43.709	151	82	2	4	151	89	239	9.18e-38	129
Msa0963310	MtrunA17_Chr5g0444791	43.624	149	77	2	7	153	19	162	1.69e-34	121

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0963310	AT4G26485.1	49.660	147	71	2	10	153	62	208	1.78e-42	140
Msa0963310	AT5G56060.1	50.365	137	64	3	7	141	15	149	1.62e-40	135
Msa0963310	AT5G56075.1	46.154	156	64	3	5	156	116	255	3.85e-38	131
Msa0963310	AT1G55790.1	43.976	166	86	2	2	160	65	230	3.64e-37	132
Msa0963310	AT5G25030.1	41.290	155	74	4	2	153	53	193	1.33e-30	110
Msa0963310	AT2G11045.1	38.411	151	56	5	5	153	21	136	1.53e-20	82.8
Msa0963310	AT1G55800.1	43.434	99	51	1	67	160	10	108	6.93e-19	80.9

Find 35 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGATCTTCCACTCAGTAAA+TGG	0.272354	6_3:-87799958	None:intergenic
TGTTATCATAGTTTCCCTAT+TGG	0.304013	6_3:+87800081	Msa0963310:CDS
TGTGCATAACTTACAGAATC+TGG	0.305326	6_3:-87799705	None:intergenic
AAAGTACTACTCTTTCCAAT+AGG	0.320713	6_3:-87800096	None:intergenic
GAGTTTCTAGTGCGGAAGTA+TGG	0.345670	6_3:+87799520	None:intergenic
TTGATTGAACTTGAGGGTCT+TGG	0.397209	6_3:+87799553	Msa0963310:CDS
TGTAGCCGGTGGCGGTTGTA+AGG	0.400513	6_3:+87800159	Msa0963310:CDS
ATTTATTATTCTTGAGATGC+TGG	0.407798	6_3:-87799621	None:intergenic
TATAATAACAAGAGAGGATC+TGG	0.413996	6_3:+87800051	Msa0963310:CDS
ATTGGAGTTTATTGAAGAAG+TGG	0.422371	6_3:+87800002	Msa0963310:CDS
AAGTACTACTCTTTCCAATA+GGG	0.433046	6_3:-87800095	None:intergenic
ATTGAAGAAGTGGAGTTTCA+AGG	0.461497	6_3:+87800012	Msa0963310:CDS
AATCTGGCAATGGTGAAATT+CGG	0.473775	6_3:-87799689	None:intergenic
CCTCTCTTGTTATTATAACC+TGG	0.475242	6_3:-87800045	None:intergenic
TGAGTGGAAGATCAAGGATC+TGG	0.510873	6_3:+87799966	Msa0963310:CDS
TAGCAACTTGATTGAACTTG+AGG	0.511290	6_3:+87799546	Msa0963310:CDS
GATTAGTGAGTGGATTTCTA+CGG	0.516725	6_3:+87799875	Msa0963310:CDS
TCTGGCTAAAGATGAGGGAT+TGG	0.524400	6_3:+87799984	Msa0963310:CDS
TTATTGATTGTAGCCGGTGG+CGG	0.526880	6_3:+87800151	Msa0963310:CDS
CCGGGCCTTACAACCGCCAC+CGG	0.536377	6_3:-87800164	None:intergenic
TAAAATTGCAGGCTTCATAG+AGG	0.536898	6_3:+87799853	Msa0963310:intron
AGCAACTTGATTGAACTTGA+GGG	0.556641	6_3:+87799547	Msa0963310:CDS
ATTTACTGAGTGGAAGATCA+AGG	0.558209	6_3:+87799960	Msa0963310:CDS
AACATTCACTTCGTGCAAGA+TGG	0.561233	6_3:-87799581	None:intergenic
TAGTACTTTCAAATTTGCCA+AGG	0.578937	6_3:+87800110	Msa0963310:CDS
ATGAGGTTATTGATTGTAGC+CGG	0.578986	6_3:+87800145	Msa0963310:CDS
CAAGGATCTGGCTAAAGATG+AGG	0.592138	6_3:+87799978	Msa0963310:CDS
CCAGGTTATAATAACAAGAG+AGG	0.602479	6_3:+87800045	Msa0963310:CDS
CGAAGTGAATGTTCACAACA+TGG	0.605758	6_3:+87799591	Msa0963310:CDS
AAGGATCTGGCTAAAGATGA+GGG	0.612540	6_3:+87799979	Msa0963310:CDS
CTTCATAGAGGATTAGTGAG+TGG	0.613571	6_3:+87799865	Msa0963310:CDS
GTAAAATTGAAACCAGCATG+TGG	0.613932	6_3:-87799667	None:intergenic
AGGTTATTGATTGTAGCCGG+TGG	0.629115	6_3:+87800148	Msa0963310:CDS
TAACTTACAGAATCTGGCAA+TGG	0.641546	6_3:-87799699	None:intergenic
CAACACATCCATTTACTGAG+TGG	0.685186	6_3:+87799950	Msa0963310:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTAAAAATAAAATTGC+AGG	+	chr6_3:87799842-87799861	Msa0963310:intron	10.0%
!	ATTTATTATTCTTGAGATGC+TGG	-	chr6_3:87799624-87799643	None:intergenic	25.0%
!!	ATCTTTAATTTTCCACATGC+TGG	+	chr6_3:87799655-87799674	Msa0963310:CDS	30.0%
!	GCTAAAAACATGCTTTCTTT+TGG	+	chr6_3:87799901-87799920	Msa0963310:CDS	30.0%
!	AAAAACATGCTTTCTTTTGG+AGG	+	chr6_3:87799904-87799923	Msa0963310:CDS	30.0%
	ATTGGAGTTTATTGAAGAAG+TGG	+	chr6_3:87800002-87800021	Msa0963310:CDS	30.0%
!	TTTCAAGGATCATTTTATCC+AGG	+	chr6_3:87800027-87800046	Msa0963310:CDS	30.0%
	TATAATAACAAGAGAGGATC+TGG	+	chr6_3:87800051-87800070	Msa0963310:CDS	30.0%
	TGTTATCATAGTTTCCCTAT+TGG	+	chr6_3:87800081-87800100	Msa0963310:CDS	30.0%
	AAGTACTACTCTTTCCAATA+GGG	-	chr6_3:87800098-87800117	None:intergenic	30.0%
!	AAAGTACTACTCTTTCCAAT+AGG	-	chr6_3:87800099-87800118	None:intergenic	30.0%
	TAGTACTTTCAAATTTGCCA+AGG	+	chr6_3:87800110-87800129	Msa0963310:CDS	30.0%
!!!	ATTTGCCAAGGAGAATTTTT+TGG	+	chr6_3:87800122-87800141	Msa0963310:CDS	30.0%
	TAGCAACTTGATTGAACTTG+AGG	+	chr6_3:87799546-87799565	Msa0963310:CDS	35.0%
	AGCAACTTGATTGAACTTGA+GGG	+	chr6_3:87799547-87799566	Msa0963310:CDS	35.0%
	GTAAAATTGAAACCAGCATG+TGG	-	chr6_3:87799670-87799689	None:intergenic	35.0%
	AATCTGGCAATGGTGAAATT+CGG	-	chr6_3:87799692-87799711	None:intergenic	35.0%
	TAACTTACAGAATCTGGCAA+TGG	-	chr6_3:87799702-87799721	None:intergenic	35.0%
	TGTGCATAACTTACAGAATC+TGG	-	chr6_3:87799708-87799727	None:intergenic	35.0%
	TAAAATTGCAGGCTTCATAG+AGG	+	chr6_3:87799853-87799872	Msa0963310:intron	35.0%
!	GATTAGTGAGTGGATTTCTA+CGG	+	chr6_3:87799875-87799894	Msa0963310:CDS	35.0%
	TTGATCTTCCACTCAGTAAA+TGG	-	chr6_3:87799961-87799980	None:intergenic	35.0%
	ATTTACTGAGTGGAAGATCA+AGG	+	chr6_3:87799960-87799979	Msa0963310:CDS	35.0%
	ATTGAAGAAGTGGAGTTTCA+AGG	+	chr6_3:87800012-87800031	Msa0963310:CDS	35.0%
!!	CCTCTCTTGTTATTATAACC+TGG	-	chr6_3:87800048-87800067	None:intergenic	35.0%
	CCAGGTTATAATAACAAGAG+AGG	+	chr6_3:87800045-87800064	Msa0963310:CDS	35.0%
	CTCATCCAAAAAATTCTCCT+TGG	-	chr6_3:87800130-87800149	None:intergenic	35.0%
!!!	CAAGGAGAATTTTTTGGATG+AGG	+	chr6_3:87800128-87800147	Msa0963310:CDS	35.0%
!	ATGAGGTTATTGATTGTAGC+CGG	+	chr6_3:87800145-87800164	Msa0963310:CDS	35.0%
	TTGATTGAACTTGAGGGTCT+TGG	+	chr6_3:87799553-87799572	Msa0963310:CDS	40.0%
	AACATTCACTTCGTGCAAGA+TGG	-	chr6_3:87799584-87799603	None:intergenic	40.0%
	CGAAGTGAATGTTCACAACA+TGG	+	chr6_3:87799591-87799610	Msa0963310:CDS	40.0%
!	CTTCATAGAGGATTAGTGAG+TGG	+	chr6_3:87799865-87799884	Msa0963310:CDS	40.0%
	CAACACATCCATTTACTGAG+TGG	+	chr6_3:87799950-87799969	Msa0963310:CDS	40.0%
	AAGGATCTGGCTAAAGATGA+GGG	+	chr6_3:87799979-87799998	Msa0963310:CDS	40.0%
	CTGATACCGACATTGAGACA+CGG	+	chr6_3:87799807-87799826	Msa0963310:intron	45.0%
	GTAACACCGTGTCTCAATGT+CGG	-	chr6_3:87799816-87799835	None:intergenic	45.0%
	TGAGTGGAAGATCAAGGATC+TGG	+	chr6_3:87799966-87799985	Msa0963310:CDS	45.0%
	CAAGGATCTGGCTAAAGATG+AGG	+	chr6_3:87799978-87799997	Msa0963310:CDS	45.0%
	TCTGGCTAAAGATGAGGGAT+TGG	+	chr6_3:87799984-87800003	Msa0963310:CDS	45.0%
!	AGGTTATTGATTGTAGCCGG+TGG	+	chr6_3:87800148-87800167	Msa0963310:CDS	45.0%
!	TTATTGATTGTAGCCGGTGG+CGG	+	chr6_3:87800151-87800170	Msa0963310:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	87799523	87800179	87799523	ID=Msa0963310;Name=Msa0963310
chr6_3	mRNA	87799523	87800179	87799523	ID=Msa0963310-mRNA-1;Parent=Msa0963310;Name=Msa0963310-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0\|0\|0\|1\|1\|1\|2\|0\|168
chr6_3	exon	87799523	87799713	87799523	ID=Msa0963310-mRNA-1:exon:15936;Parent=Msa0963310-mRNA-1
chr6_3	exon	87799864	87800179	87799864	ID=Msa0963310-mRNA-1:exon:15937;Parent=Msa0963310-mRNA-1
chr6_3	CDS	87799523	87799713	87799523	ID=Msa0963310-mRNA-1:cds;Parent=Msa0963310-mRNA-1
chr6_3	CDS	87799864	87800179	87799864	ID=Msa0963310-mRNA-1:cds;Parent=Msa0963310-mRNA-1

Gene Sequence

>Msa0963310

TTTCTAGTGCGGAAGTATGGATCTAGCAACTTGATTGAACTTGAGGGTCTTGGCTGTACCATCTTGCACGAAGTGAATGTTCACAACATGGCTCAAGACCAGCATCTCAAGAATAATAAATTTGATCGAATAATCTTTAATTTTCCACATGCTGGTTTCAATTTTACCGAATTTCACCATTGCCAGATTCTGCTTCATAGAGGATTAGTGAGTGGATTTCTACGGAATGCTAAAAACATGCTTTCTTTTGGAGGAGAAATTCATATTTCTCACAAGACAACACATCCATTTACTGAGTGGAAGATCAAGGATCTGGCTAAAGATGAGGGATTGGAGTTTATTGAAGAAGTGGAGTTTCAAGGATCATTTTATCCAGGTTATAATAACAAGAGAGGATCTGGTTCTAAATGTTATCATAGTTTCCCTATTGGAAAGAGTAGTACTTTCAAATTTGCCAAGGAGAATTTTTTGGATGAGGTTATTGATTGTAGCCGGTGGCGGTTGTAA

Protein sequence

>Msa0963310

FLVRKYGSSNLIELEGLGCTILHEVNVHNMAQDQHLKNNKFDRIIFNFPHAGFNFTEFHHCQILLHRGLVSGFLRNAKNMLSFGGEIHISHKTTHPFTEWKIKDLAKDEGLEFIEEVEFQGSFYPGYNNKRGSGSKCYHSFPIGKSSTFKFAKENFLDEVIDCSRWRL*