AlfalfaGEDB | Msa0858360

M. sativa cultivar zhongmu-4 / Msa0858360

KEGG: K04730
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0858360	RHN57117.1	89.623	106	11	0	46	151	5	110	6.39e-63	202

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0858360	sp\|C0LGP4\|Y3475_ARATH	42.105	114	61	1	45	158	900	1008	2.20e-20	92.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0858360	A0A396I2P3	89.623	106	11	0	46	151	5	110	3.05e-63	202

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0021460	Msa0858360	0.803277	3.764241e-49	-8.615850e-47
Msa0023610	Msa0858360	0.820366	7.123637e-53	-8.615850e-47
Msa0033340	Msa0858360	0.806493	8.007206e-50	-8.615850e-47
Msa0068630	Msa0858360	0.802845	4.624426e-49	-8.615850e-47
Msa0071340	Msa0858360	0.825990	3.466784e-54	-8.615850e-47
Msa0109970	Msa0858360	0.804823	1.794913e-49	-8.615850e-47
Msa0109990	Msa0858360	0.816056	6.730541e-52	-8.615850e-47
Msa0618430	Msa0858360	0.841481	4.630398e-58	-8.615850e-47
Msa0629850	Msa0858360	0.815297	9.935080e-52	-8.615850e-47
Msa0629930	Msa0858360	0.817411	3.343699e-52	-8.615850e-47
Msa0629940	Msa0858360	0.831477	1.632698e-55	-8.615850e-47
Msa0668150	Msa0858360	0.824091	9.739375e-54	-8.615850e-47
Msa0675510	Msa0858360	0.809382	1.945127e-50	-8.615850e-47
Msa0682360	Msa0858360	0.801192	1.011139e-48	-8.615850e-47
Msa0702870	Msa0858360	0.816963	4.217535e-52	-8.615850e-47
Msa0754180	Msa0858360	0.825053	5.781813e-54	-8.615850e-47
Msa0774590	Msa0858360	0.805615	1.225287e-49	-8.615850e-47
Msa0784520	Msa0858360	0.874202	8.121107e-68	-8.615850e-47
Msa0784580	Msa0858360	0.829653	4.563048e-55	-8.615850e-47
Msa0815320	Msa0858360	0.852946	3.305635e-61	-8.615850e-47
Msa0818010	Msa0858360	0.855895	4.640887e-62	-8.615850e-47
Msa0818030	Msa0858360	0.902218	1.213798e-78	-8.615850e-47
Msa0137330	Msa0858360	0.826763	2.269864e-54	-8.615850e-47
Msa0142230	Msa0858360	0.823556	1.299723e-53	-8.615850e-47
Msa0153200	Msa0858360	0.833097	6.484084e-56	-8.615850e-47
Msa0201390	Msa0858360	0.801500	8.744464e-49	-8.615850e-47
Msa0203070	Msa0858360	0.810402	1.173564e-50	-8.615850e-47
Msa0211680	Msa0858360	0.876704	1.130127e-68	-8.615850e-47
Msa0235190	Msa0858360	0.841621	4.252669e-58	-8.615850e-47
Msa0365810	Msa0858360	0.815195	1.046976e-51	-8.615850e-47
Msa0391050	Msa0858360	0.809875	1.524323e-50	-8.615850e-47
Msa0412800	Msa0858360	0.816071	6.678640e-52	-8.615850e-47
Msa0414590	Msa0858360	0.843551	1.306777e-58	-8.615850e-47
Msa0452410	Msa0858360	0.812668	3.775991e-51	-8.615850e-47
Msa0458700	Msa0858360	0.857812	1.264409e-62	-8.615850e-47
Msa0273560	Msa0858360	0.800258	1.568416e-48	-8.615850e-47
Msa0273570	Msa0858360	0.835815	1.346841e-56	-8.615850e-47
Msa0273580	Msa0858360	0.804578	2.020138e-49	-8.615850e-47
Msa0280450	Msa0858360	0.802322	5.928173e-49	-8.615850e-47
Msa0284810	Msa0858360	0.812494	4.121102e-51	-8.615850e-47
Msa0291600	Msa0858360	0.800849	1.188362e-48	-8.615850e-47
Msa0313780	Msa0858360	0.803912	2.779183e-49	-8.615850e-47
Msa0313920	Msa0858360	0.807223	5.615161e-50	-8.615850e-47
Msa0323550	Msa0858360	0.810574	1.077011e-50	-8.615850e-47
Msa0352000	Msa0858360	0.801166	1.023598e-48	-8.615850e-47
Msa0352010	Msa0858360	0.803194	3.915980e-49	-8.615850e-47
Msa0352280	Msa0858360	0.831544	1.571534e-55	-8.615850e-47
Msa0353690	Msa0858360	0.804649	1.952283e-49	-8.615850e-47
Msa0362860	Msa0858360	0.817470	3.243320e-52	-8.615850e-47
Msa0506000	Msa0858360	0.819207	1.310867e-52	-8.615850e-47
Msa0533290	Msa0858360	0.853752	1.941154e-61	-8.615850e-47
Msa0533430	Msa0858360	0.868357	6.935871e-66	-8.615850e-47
Msa0580610	Msa0858360	0.856032	4.230480e-62	-8.615850e-47
Msa0584130	Msa0858360	0.811440	6.994694e-51	-8.615850e-47
Msa0858360	Msa0865390	0.806645	7.438052e-50	-8.615850e-47
Msa0858360	Msa0883370	0.830620	2.650190e-55	-8.615850e-47
Msa0858360	Msa0884920	0.823799	1.139972e-53	-8.615850e-47
Msa0858360	Msa0884980	0.811464	6.908827e-51	-8.615850e-47
Msa0858360	Msa0902020	0.848631	5.419060e-60	-8.615850e-47
Msa0858360	Msa0912370	0.832036	1.188774e-55	-8.615850e-47
Msa0858360	Msa0912500	0.860896	1.500445e-63	-8.615850e-47
Msa0858360	Msa0924370	0.867769	1.071610e-65	-8.615850e-47
Msa0858360	Msa0963310	0.848534	5.765279e-60	-8.615850e-47
Msa0858360	Msa0965750	0.804558	2.039450e-49	-8.615850e-47
Msa0858360	Msa1006250	0.878461	2.757614e-69	-8.615850e-47
Msa0858360	Msa1006390	0.816305	5.921581e-52	-8.615850e-47
Msa0858360	Msa1006400	0.818457	1.940574e-52	-8.615850e-47
Msa0858360	Msa1020720	0.807622	4.620713e-50	-8.615850e-47
Msa0858360	Msa1027240	0.840418	8.798599e-58	-8.615850e-47
Msa0858360	Msa1048490	0.822989	1.763483e-53	-8.615850e-47
Msa0858360	Msa1048590	0.816714	4.797310e-52	-8.615850e-47
Msa0858360	Msa1048610	0.838985	2.077186e-57	-8.615850e-47
Msa0858360	Msa1050480	0.829789	4.228125e-55	-8.615850e-47
Msa0858360	Msa1072490	0.803865	2.842000e-49	-8.615850e-47
Msa0858360	Msa1100180	0.824188	9.240774e-54	-8.615850e-47
Msa0858360	Msa1107800	0.813070	3.083433e-51	-8.615850e-47
Msa0858360	Msa1131820	0.837482	5.063390e-57	-8.615850e-47
Msa0858360	Msa1187060	0.804237	2.379148e-49	-8.615850e-47
Msa0858360	Msa1198090	0.805453	1.325025e-49	-8.615850e-47
Msa0858360	Msa1232160	0.816975	4.190590e-52	-8.615850e-47
Msa0858360	Msa1287560	0.828000	1.146135e-54	-8.615850e-47
Msa0858360	Msa1287700	0.817107	3.913753e-52	-8.615850e-47
Msa0858360	Msa1288000	0.872436	3.185424e-67	-8.615850e-47
Msa0858360	Msa1324780	0.813737	2.199958e-51	-8.615850e-47
Msa0858360	Msa1351080	0.825247	5.202359e-54	-8.615850e-47
Msa0858360	Msa1359820	0.853178	2.836965e-61	-8.615850e-47
Msa0858360	Msa1406600	0.820924	5.302635e-53	-8.615850e-47
Msa0858360	Msa1423990	0.866390	2.954049e-65	-8.615850e-47
Msa0858360	Msa1444370	0.860541	1.922063e-63	-8.615850e-47
Msa0858360	Msa1463140	0.820287	7.429733e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0858360	MtrunA17_Chr5g0436931	89.623	106	11	0	46	151	5	110	5.88e-67	202
Msa0858360	MtrunA17_Chr5g0436861	88.785	107	12	0	46	152	902	1008	1.26e-62	210
Msa0858360	MtrunA17_Chr5g0437331	85.849	106	15	0	46	151	899	1004	6.86e-57	194
Msa0858360	MtrunA17_Chr7g0248261	78.505	107	23	0	46	152	853	959	2.08e-53	184
Msa0858360	MtrunA17_Chr5g0436911	74.766	107	27	0	46	152	184	290	1.21e-52	171
Msa0858360	MtrunA17_Chr5g0436851	74.528	106	27	0	47	152	50	155	1.71e-51	164
Msa0858360	MtrunA17_Chr1g0159871	75.701	107	26	0	46	152	829	935	2.42e-49	172
Msa0858360	MtrunA17_Chr5g0436871	71.963	107	30	0	46	152	104	210	1.25e-48	159
Msa0858360	MtrunA17_Chr5g0436841	76.415	106	20	1	46	151	948	1048	1.19e-47	168
Msa0858360	MtrunA17_Chr1g0159881	77.000	100	23	0	46	145	370	469	8.21e-47	161
Msa0858360	MtrunA17_Chr2g0314001	70.755	106	31	0	47	152	903	1008	8.36e-46	162
Msa0858360	MtrunA17_Chr2g0314021	71.698	106	30	0	47	152	903	1008	9.85e-46	162
Msa0858360	MtrunA17_Chr3g0078621	73.404	94	25	0	59	152	1	94	1.22e-44	144
Msa0858360	MtrunA17_Chr5g0436941	64.151	106	37	1	46	151	896	1000	6.51e-39	143
Msa0858360	MtrunA17_Chr2g0314031	63.208	106	30	2	47	152	899	995	1.32e-34	130
Msa0858360	MtrunA17_Chr8g0366461	42.991	107	58	1	47	153	906	1009	1.04e-23	99.4
Msa0858360	MtrunA17_Chr8g0369631	41.228	114	61	2	45	152	7	120	2.26e-21	85.9
Msa0858360	MtrunA17_Chr5g0408401	39.640	111	63	1	46	152	674	784	3.24e-21	92.0
Msa0858360	MtrunA17_Chr3g0113221	41.818	110	62	1	45	152	899	1008	1.09e-20	90.5
Msa0858360	MtrunA17_Chr8g0367351	44.348	115	58	2	45	153	905	1019	1.92e-20	89.7
Msa0858360	MtrunA17_Chr8g0352041	62.295	61	23	0	48	108	48	108	8.28e-20	81.6
Msa0858360	MtrunA17_Chr5g0408501	43.519	108	56	2	47	149	9	116	8.48e-20	82.0
Msa0858360	MtrunA17_Chr5g0408811	44.860	107	53	3	47	149	883	987	9.10e-20	87.8
Msa0858360	MtrunA17_Chr5g0408741	42.453	106	56	2	47	149	816	919	1.61e-19	87.0
Msa0858360	MtrunA17_Chr5g0408581	42.593	108	57	2	47	149	912	1019	2.24e-19	86.7
Msa0858360	MtrunA17_Chr1g0196011	39.286	112	62	1	46	151	737	848	4.82e-19	85.5
Msa0858360	MtrunA17_Chr2g0300201	43.119	109	55	2	47	149	912	1019	5.30e-19	85.5
Msa0858360	MtrunA17_Chr6g0464211	42.202	109	56	2	47	149	911	1018	7.06e-19	85.1
Msa0858360	MtrunA17_Chr5g0408621	40.909	110	56	2	47	149	946	1053	1.38e-18	84.3
Msa0858360	MtrunA17_Chr6g0464141	40.909	110	57	2	47	149	912	1020	1.69e-18	84.0
Msa0858360	MtrunA17_Chr5g0419221	35.185	108	65	1	47	149	911	1018	7.03e-18	82.4
Msa0858360	MtrunA17_Chr1g0196031	38.393	112	63	1	46	151	945	1056	1.54e-17	81.3
Msa0858360	MtrunA17_Chr6g0472051	40.909	88	52	0	47	134	116	203	2.21e-17	77.4
Msa0858360	MtrunA17_Chr6g0464161	38.889	108	61	1	47	149	641	748	3.26e-17	80.1
Msa0858360	MtrunA17_Chr6g0464171	38.889	108	61	2	47	149	912	1019	9.51e-17	79.0
Msa0858360	MtrunA17_Chr5g0408431	42.202	109	57	3	47	150	910	1017	1.42e-16	78.6
Msa0858360	MtrunA17_Chr5g0408611	38.889	108	61	1	47	149	899	1006	2.64e-16	77.8
Msa0858360	MtrunA17_Chr5g0408821	38.182	110	63	2	46	150	304	413	3.05e-16	77.0
Msa0858360	MtrunA17_Chr5g0408801	39.815	108	60	2	47	149	912	1019	3.17e-16	77.4
Msa0858360	MtrunA17_Chr5g0408571	38.053	113	59	2	47	151	390	499	3.19e-16	77.0
Msa0858360	MtrunA17_Chr5g0408421	37.963	108	62	2	47	149	893	1000	4.19e-16	77.0
Msa0858360	MtrunA17_Chr5g0403401	37.383	107	62	1	47	148	900	1006	6.25e-16	76.6
Msa0858360	MtrunA17_Chr8g0362391	38.462	104	59	2	45	148	225	323	7.32e-16	75.5
Msa0858360	MtrunA17_Chr8g0360851	34.615	104	63	1	45	148	907	1005	1.11e-15	75.9
Msa0858360	MtrunA17_Chr8g0363191	38.318	107	55	3	45	148	600	698	2.92e-15	74.3
Msa0858360	MtrunA17_Chr8g0363111	35.514	107	58	2	45	148	883	981	3.71e-15	74.3
Msa0858360	MtrunA17_Chr7g0241921	33.333	120	65	2	47	151	883	1002	3.93e-15	74.3
Msa0858360	MtrunA17_Chr5g0408511	38.384	99	60	1	46	144	907	1004	4.25e-15	73.9
Msa0858360	MtrunA17_Chr8g0362911	34.615	104	63	2	45	148	622	720	4.73e-15	73.9
Msa0858360	MtrunA17_Chr8g0363641	35.577	104	62	2	45	148	150	248	7.05e-15	71.6
Msa0858360	MtrunA17_Chr8g0363371	35.514	107	58	2	45	148	727	825	8.86e-15	73.2
Msa0858360	MtrunA17_Chr8g0361231	36.792	106	58	2	45	148	975	1073	1.31e-14	72.8
Msa0858360	MtrunA17_Chr8g0362871	35.577	104	62	2	45	148	673	771	1.48e-14	72.4
Msa0858360	MtrunA17_Chr8g0363061	38.318	107	55	3	45	148	879	977	1.74e-14	72.4
Msa0858360	MtrunA17_Chr8g0363401	35.577	104	62	2	45	148	688	786	2.29e-14	72.0
Msa0858360	MtrunA17_Chr5g0407241	35.849	106	57	2	47	144	917	1019	2.42e-14	72.0
Msa0858360	MtrunA17_Chr8g0363601	36.538	104	61	2	45	148	673	771	2.51e-14	71.6
Msa0858360	MtrunA17_Chr8g0363691	36.538	104	61	2	45	148	1147	1245	2.74e-14	71.6
Msa0858360	MtrunA17_Chr8g0362431	34.615	104	63	2	45	148	681	779	2.92e-14	71.6
Msa0858360	MtrunA17_Chr8g0362941	34.951	103	62	2	45	147	639	736	3.02e-14	71.6
Msa0858360	MtrunA17_Chr8g0362961	36.449	107	57	3	45	148	1123	1221	3.36e-14	71.6
Msa0858360	MtrunA17_Chr2g0284121	34.821	112	62	2	45	153	1112	1215	3.83e-14	71.2
Msa0858360	MtrunA17_Chr8g0363661	36.538	104	61	2	45	148	736	834	5.58e-14	70.9
Msa0858360	MtrunA17_Chr1g0176301	35.849	106	59	2	45	148	638	736	5.73e-14	70.9
Msa0858360	MtrunA17_Chr8g0363261	35.514	107	58	2	45	148	283	381	6.46e-14	70.1
Msa0858360	MtrunA17_Chr1g0196051	57.143	49	21	0	48	96	17	65	7.39e-14	64.7
Msa0858360	MtrunA17_Chr6g0464201	43.662	71	40	0	47	117	51	121	7.70e-14	66.2
Msa0858360	MtrunA17_Chr8g0363281	34.951	103	62	2	45	147	656	753	7.87e-14	70.5
Msa0858360	MtrunA17_Chr8g0363121	33.654	104	64	2	45	148	683	781	1.07e-13	70.1
Msa0858360	MtrunA17_Chr4g0013491	34.615	104	62	1	45	148	636	733	1.16e-13	69.7
Msa0858360	MtrunA17_Chr8g0362931	34.615	104	63	1	45	148	1068	1166	1.25e-13	69.7
Msa0858360	MtrunA17_Chr8g0363221	32.710	107	61	2	45	148	753	851	2.13e-13	68.9
Msa0858360	MtrunA17_Chr8g0363501	35.514	107	58	2	45	148	419	517	4.18e-13	68.2
Msa0858360	MtrunA17_Chr1g0196001	50.847	59	26	1	46	104	905	960	6.74e-13	67.8
Msa0858360	MtrunA17_Chr8g0362841	33.645	107	60	2	45	148	725	823	7.82e-13	67.4
Msa0858360	MtrunA17_Chr8g0362981	33.981	103	63	2	45	147	639	736	1.08e-12	67.0
Msa0858360	MtrunA17_Chr8g0363241	33.654	104	64	2	45	148	641	739	1.14e-12	67.0
Msa0858360	MtrunA17_Chr3g0095201	30.769	104	67	2	45	148	887	985	1.18e-12	67.0
Msa0858360	MtrunA17_Chr3g0127701	29.032	124	67	1	47	149	871	994	7.77e-12	64.3
Msa0858360	MtrunA17_Chr2g0284191	33.645	107	59	2	45	151	742	836	2.12e-11	63.2
Msa0858360	MtrunA17_Chr7g0262141	34.579	107	58	2	46	150	896	992	2.92e-11	62.8
Msa0858360	MtrunA17_Chr2g0284151	34.579	107	58	2	45	151	696	790	4.15e-11	62.4
Msa0858360	MtrunA17_Chr3g0127671	26.984	126	71	1	47	151	916	1041	7.96e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0858360	AT3G47580.1	40.187	107	59	1	45	151	901	1002	1.61e-22	96.3
Msa0858360	AT3G47570.1	42.105	114	61	1	45	158	900	1008	2.24e-21	92.8
Msa0858360	AT3G47110.1	39.450	109	58	3	45	151	917	1019	1.13e-18	85.1
Msa0858360	AT3G47090.1	37.383	107	62	1	45	151	899	1000	3.50e-18	83.6
Msa0858360	AT5G20480.1	35.514	107	53	2	45	151	910	1000	3.76e-16	77.8
Msa0858360	AT5G20480.2	35.514	107	53	2	45	151	910	1000	3.76e-16	77.8
Msa0858360	AT5G39390.1	36.449	107	52	2	45	151	407	497	2.13e-15	75.1
Msa0858360	AT2G24130.1	31.481	108	74	0	47	154	856	963	9.60e-13	67.4
Msa0858360	AT2G24130.2	31.481	108	74	0	47	154	871	978	9.63e-13	67.4

Find 46 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCATAGAAACAACAAGTTT+TGG	0.277671	5_4:+55726807	Msa0858360:CDS
CTTCTGTTAGAGATGCTTAC+TGG	0.311174	5_4:+55724141	Msa0858360:CDS
AGAAGGAATTCTTGATATAG+TGG	0.358148	5_4:+55724233	Msa0858360:CDS
TTCTCAATTCTCTGACCTGC+TGG	0.391989	5_4:+55726736	Msa0858360:CDS
GAGTCACCATGTGGAGACTA+TGG	0.403867	5_4:+55726783	Msa0858360:CDS
TTTCTGCCAACTTGTGAGTA+AGG	0.406828	5_4:-55726875	None:intergenic
CTAGTGATGTTTGCTAAAAT+AGG	0.420102	5_4:+55724324	Msa0858360:CDS
TGGAAGGAGCTCCTTGCCAC+TGG	0.438789	5_4:+55723961	Msa0858360:CDS
GTATTCCCCGGGTACGTCCT+TGG	0.446434	5_4:+55723941	Msa0858360:CDS
TAACCACAAACCAAGTTGCT+TGG	0.449754	5_4:-55723987	None:intergenic
AGGACGTACCCGGGGAATAC+CGG	0.450246	5_4:-55723938	None:intergenic
TGATGAAGAAGAGGAAATGA+AGG	0.454044	5_4:+55727896	Msa0858360:CDS
CCACTGGAGCCCAAGCAACT+TGG	0.454751	5_4:+55723977	Msa0858360:CDS
AGCCCAAGCAACTTGGTTTG+TGG	0.455575	5_4:+55723984	Msa0858360:CDS
ACTATATCAAGAATTCCTTC+TGG	0.459349	5_4:-55724231	None:intergenic
GAATACCGGGTTCGATTCCT+AGG	0.462841	5_4:-55723924	None:intergenic
AACCACAAACCAAGTTGCTT+GGG	0.464654	5_4:-55723986	None:intergenic
GAATCGAACCCGGTATTCCC+CGG	0.497345	5_4:+55723929	Msa0858360:CDS
GCTCCTTCCAAGGACGTACC+CGG	0.512343	5_4:-55723948	None:intergenic
TTCCTAGGAGGAACAACGCT+TGG	0.517554	5_4:-55723909	None:intergenic
TTTGTGAGCATTCTCTGAGT+AGG	0.520486	5_4:-55724369	None:intergenic
CTCCTTCCAAGGACGTACCC+GGG	0.522932	5_4:-55723947	None:intergenic
CTCACAAGTTGGCAGAAACA+AGG	0.523777	5_4:+55726880	Msa0858360:CDS
TCTCTGAGTAGGAAATTCTT+CGG	0.530661	5_4:-55724358	None:intergenic
GGACGTACCCGGGGAATACC+GGG	0.531957	5_4:-55723937	None:intergenic
CAGGGAGATATCTATAGCTA+TGG	0.537934	5_4:+55724114	Msa0858360:intron
CCAAGTTGCTTGGGCTCCAG+TGG	0.540069	5_4:-55723977	None:intergenic
TCCCCGGGTACGTCCTTGGA+AGG	0.561053	5_4:+55723945	Msa0858360:CDS
ATGCGTAGAGGCATGCACAC+TGG	0.569466	5_4:+55723886	Msa0858360:CDS
GGCCAAGCGTTGTTCCTCCT+AGG	0.576716	5_4:+55723907	Msa0858360:CDS
TCGACTCCTTACTCACAAGT+TGG	0.583826	5_4:+55726869	Msa0858360:CDS
TTGCTTGGGCTCCAGTGGCA+AGG	0.590024	5_4:-55723972	None:intergenic
TGTTGAAGATGATGAAGAAG+AGG	0.598263	5_4:+55727887	Msa0858360:CDS
AATCGAACCCGGTATTCCCC+GGG	0.599401	5_4:+55723930	Msa0858360:CDS
TGTAAGATGAAAATTCCAGA+AGG	0.604286	5_4:+55724216	Msa0858360:CDS
GTGGCAAGGAGCTCCTTCCA+AGG	0.605596	5_4:-55723958	None:intergenic
CTAATCCGGCTCATGCGTAG+AGG	0.628361	5_4:+55723874	None:intergenic
GCAGCCTATGTACTCTTGTG+CGG	0.629094	5_4:+55726705	Msa0858360:CDS
TAATCCGCACAAGAGTACAT+AGG	0.629845	5_4:-55726709	None:intergenic
TCCTTCCAAGGACGTACCCG+GGG	0.630546	5_4:-55723946	None:intergenic
TTCCTCCTAGGAATCGAACC+CGG	0.641197	5_4:+55723919	Msa0858360:CDS
TTGTGAGTAAGGAGTCGACT+CGG	0.656594	5_4:-55726864	None:intergenic
TACCGGGTTCGATTCCTAGG+AGG	0.669923	5_4:-55723921	None:intergenic
TGAAGATCAAACACGTGTTG+TGG	0.672054	5_4:+55724281	Msa0858360:CDS
AAGTATTTGGAGTCACCATG+TGG	0.691429	5_4:+55726774	Msa0858360:CDS
ATGCTTCCATAGTCTCCACA+TGG	0.694977	5_4:-55726789	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTGTTATTGAATATAAAAA+AGG	+	chr5_4:55724710-55724729	Msa0858360:intron	10.0%
!!!	AGAAATTTTTTAAAAATTTG+GGG	+	chr5_4:55725005-55725024	Msa0858360:intron	10.0%
!!!	TAATTTAAAGATTTTCAAAA+TGG	+	chr5_4:55726351-55726370	Msa0858360:intron	10.0%
!!!	TATTTTTTGATAAAATTGAA+GGG	-	chr5_4:55726554-55726573	None:intergenic	10.0%
!!!	ATATTTTTTGATAAAATTGA+AGG	-	chr5_4:55726555-55726574	None:intergenic	10.0%
!!	GTTACATTATATTATACATA+TGG	+	chr5_4:55724565-55724584	Msa0858360:intron	15.0%
!!	GTTCTTACATAAAAAATATT+TGG	-	chr5_4:55725043-55725062	None:intergenic	15.0%
!!	CAGATTAAATATTAAATATG+TGG	+	chr5_4:55725512-55725531	Msa0858360:intron	15.0%
!!	GCTGAATATAATTATTATTA+TGG	+	chr5_4:55725630-55725649	Msa0858360:intron	15.0%
!!!	AATTTTTTGGAAATTTATTG+GGG	-	chr5_4:55726522-55726541	None:intergenic	15.0%
!!!	CAATTTTTTGGAAATTTATT+GGG	-	chr5_4:55726523-55726542	None:intergenic	15.0%
!!!	ACAATTTTTTGGAAATTTAT+TGG	-	chr5_4:55726524-55726543	None:intergenic	15.0%
!!	TCAAATTAAAAAAAATTCGA+GGG	-	chr5_4:55726586-55726605	None:intergenic	15.0%
!!	TTCAAATTAAAAAAAATTCG+AGG	-	chr5_4:55726587-55726606	None:intergenic	15.0%
!!	AAATTTGAAAATTGAAATTG+TGG	-	chr5_4:55726616-55726635	None:intergenic	15.0%
!!!	ACAATTTCAATTTTCAAATT+TGG	+	chr5_4:55726615-55726634	Msa0858360:intron	15.0%
!!!	TATTATTTTCTTATTTTGTG+TGG	+	chr5_4:55727306-55727325	Msa0858360:intron	15.0%
!!	AAAATAAAAATAGAAACTAC+AGG	+	chr5_4:55727549-55727568	Msa0858360:intron	15.0%
!!!	TACTTTTAAGTAAGTTAAAT+GGG	+	chr5_4:55727749-55727768	Msa0858360:intron	15.0%
!!	CTACTTTGATAAGAAAATAA+TGG	+	chr5_4:55724450-55724469	Msa0858360:intron	20.0%
!!	TTATACATATGGTATTTGTT+TGG	+	chr5_4:55724576-55724595	Msa0858360:intron	20.0%
!!	ATTTAATGTCAGACATATTT+AGG	-	chr5_4:55725065-55725084	None:intergenic	20.0%
!!!	TGAAAGACTTAACTTTAAAA+AGG	-	chr5_4:55725179-55725198	None:intergenic	20.0%
!!	ATAGAATAACTATATGCAAT+TGG	+	chr5_4:55725379-55725398	Msa0858360:intron	20.0%
!!	TTACTTCTTTAAATGTTTGT+AGG	-	chr5_4:55726011-55726030	None:intergenic	20.0%
!!	AAATCAAATAAAAAGGAGTA+TGG	-	chr5_4:55726113-55726132	None:intergenic	20.0%
!!	GGAAATAATTGAAAAGAATA+TGG	-	chr5_4:55726197-55726216	None:intergenic	20.0%
!!	AAACTAAATTAGAGTGTATT+TGG	+	chr5_4:55726271-55726290	Msa0858360:intron	20.0%
!!!	TTTGAAGTGAATTTATTGAT+TGG	+	chr5_4:55726324-55726343	Msa0858360:intron	20.0%
!!!	TTTAAAGATTTTCAAAATGG+TGG	+	chr5_4:55726354-55726373	Msa0858360:intron	20.0%
!!!	AGTTTTCAAAATTTGCATTT+TGG	+	chr5_4:55726466-55726485	Msa0858360:intron	20.0%
!!	CAAATTAAAAAAAATTCGAG+GGG	-	chr5_4:55726585-55726604	None:intergenic	20.0%
!!!	TTTCATATAACACTTGTTTT+TGG	-	chr5_4:55726642-55726661	None:intergenic	20.0%
!!!	TTTTGAAATGGACTTTAAAT+AGG	+	chr5_4:55727181-55727200	Msa0858360:intron	20.0%
!!	TTATTGTCATAAATACGTAA+TGG	-	chr5_4:55727237-55727256	None:intergenic	20.0%
!!	AATAAGTGCATTAACAATTT+CGG	+	chr5_4:55727374-55727393	Msa0858360:intron	20.0%
!!!	CTACTTTTAAGTAAGTTAAA+TGG	+	chr5_4:55727748-55727767	Msa0858360:intron	20.0%
!!!	ACTTTTAAGTAAGTTAAATG+GGG	+	chr5_4:55727750-55727769	Msa0858360:intron	20.0%
!!!	ATGAATTTCTGGATTTTTTT+GGG	+	chr5_4:55727839-55727858	Msa0858360:intron	20.0%
!	CTTAAAAACATATCTGTACA+TGG	-	chr5_4:55724740-55724759	None:intergenic	25.0%
!	TTCATTTAAATGTTCCTTGA+GGG	-	chr5_4:55724781-55724800	None:intergenic	25.0%
!	GATCAAAATCACATATACTT+TGG	-	chr5_4:55724926-55724945	None:intergenic	25.0%
!	TGATCATTTAATTGAGAAAG+AGG	+	chr5_4:55724941-55724960	Msa0858360:intron	25.0%
!	AAAGTTAAGTCTTTCATTCA+TGG	+	chr5_4:55725183-55725202	Msa0858360:intron	25.0%
!	TTTATTTATACCGTGACTAA+AGG	+	chr5_4:55725484-55725503	Msa0858360:intron	25.0%
!	TTATTTATACCGTGACTAAA+GGG	+	chr5_4:55725485-55725504	Msa0858360:intron	25.0%
!	AAATAATTGTATTCAGCAGA+TGG	-	chr5_4:55725593-55725612	None:intergenic	25.0%
!	TAACAAGTAAGAAACATTGT+TGG	+	chr5_4:55725933-55725952	Msa0858360:intron	25.0%
!	ATAAAACCTAACCTAAAGTT+TGG	+	chr5_4:55725967-55725986	Msa0858360:intron	25.0%
!	TTCTTTCTTTACCAAACTTT+AGG	-	chr5_4:55725981-55726000	None:intergenic	25.0%
!	TAAAGTTTGGTAAAGAAAGA+AGG	+	chr5_4:55725980-55725999	Msa0858360:intron	25.0%
!	TCCTCACGTAAAATAATTAT+TGG	+	chr5_4:55726078-55726097	Msa0858360:intron	25.0%
!!!	ACCAATAATTATTTTACGTG+AGG	-	chr5_4:55726082-55726101	None:intergenic	25.0%
!!	TTTTCAATTATTTCCGTGAA+AGG	+	chr5_4:55726202-55726221	Msa0858360:intron	25.0%
!	GTAATTGTTTGCATAAAGTA+TGG	+	chr5_4:55726230-55726249	Msa0858360:intron	25.0%
!	TAAATTAGAGTGTATTTGGA+CGG	+	chr5_4:55726275-55726294	Msa0858360:intron	25.0%
!!!	TGAAAATTTTGGAAAAGTTG+AGG	-	chr5_4:55726493-55726512	None:intergenic	25.0%
!!!	AGGGTCAATTTACAATTTTT+TGG	-	chr5_4:55726535-55726554	None:intergenic	25.0%
!	GATGAATATGAATTTCGAAA+TGG	+	chr5_4:55727115-55727134	Msa0858360:intron	25.0%
!!!	TGATAGTGGTACATAATAAT+TGG	-	chr5_4:55727409-55727428	None:intergenic	25.0%
!	ATCAGCATATATTCTTGATA+AGG	-	chr5_4:55727698-55727717	None:intergenic	25.0%
!!	AATGTTTGTTGATGAATTTC+TGG	+	chr5_4:55727828-55727847	Msa0858360:intron	25.0%
!!!	GATGAATTTCTGGATTTTTT+TGG	+	chr5_4:55727838-55727857	Msa0858360:intron	25.0%
!!	CTTGTTGTTTGAATAGAGTA+TGG	+	chr5_4:55724069-55724088	Msa0858360:intron	30.0%
!!	TCACAAAAAGTGTTATCTGT+TGG	-	chr5_4:55724174-55724193	None:intergenic	30.0%
	TGTAAGATGAAAATTCCAGA+AGG	+	chr5_4:55724216-55724235	Msa0858360:CDS	30.0%
	ACTATATCAAGAATTCCTTC+TGG	-	chr5_4:55724234-55724253	None:intergenic	30.0%
	AGAAGGAATTCTTGATATAG+TGG	+	chr5_4:55724233-55724252	Msa0858360:CDS	30.0%
!	CTAGTGATGTTTGCTAAAAT+AGG	+	chr5_4:55724324-55724343	Msa0858360:CDS	30.0%
	CAAAACTAAAAACACACGTA+AGG	-	chr5_4:55724508-55724527	None:intergenic	30.0%
	AAATGCCGTTAATTATGCAA+GGG	+	chr5_4:55724599-55724618	Msa0858360:intron	30.0%
	ACAATCCCTTGCATAATTAA+CGG	-	chr5_4:55724607-55724626	None:intergenic	30.0%
	TAATTATGCAAGGGATTGTA+TGG	+	chr5_4:55724608-55724627	Msa0858360:intron	30.0%
	GTTCATTTAAATGTTCCTTG+AGG	-	chr5_4:55724782-55724801	None:intergenic	30.0%
	CTGTTAGCACAAAGAATATT+CGG	+	chr5_4:55725243-55725262	Msa0858360:intron	30.0%
!!!	TTTTCTGGAATAAAGGATTG+TGG	-	chr5_4:55725737-55725756	None:intergenic	30.0%
	ATCCTTTATTCCAGAAAATC+CGG	+	chr5_4:55725739-55725758	Msa0858360:intron	30.0%
!	CATACATGTTAAATAGTGGA+AGG	-	chr5_4:55725774-55725793	None:intergenic	30.0%
	GAAGCATACATGTTAAATAG+TGG	-	chr5_4:55725778-55725797	None:intergenic	30.0%
!	AATAATGCTATTTAGTGCGA+AGG	-	chr5_4:55725822-55725841	None:intergenic	30.0%
!!	GTTTCTTACTTGTTATCTAG+AGG	-	chr5_4:55725928-55725947	None:intergenic	30.0%
!!!	TTAGGTTAGGTTTTATTCGA+AGG	-	chr5_4:55725963-55725982	None:intergenic	30.0%
!	TCTTTACCAAACTTTAGGTT+AGG	-	chr5_4:55725976-55725995	None:intergenic	30.0%
	ACAATTACTTGTTCCTTTCA+CGG	-	chr5_4:55726218-55726237	None:intergenic	30.0%
	AAATTAGAGTGTATTTGGAC+GGG	+	chr5_4:55726276-55726295	Msa0858360:intron	30.0%
!	TGGGGTCAATTTGAAAATTT+TGG	-	chr5_4:55726504-55726523	None:intergenic	30.0%
!!!	TCTTTGAAATGCAATTTTGC+AGG	+	chr5_4:55726678-55726697	Msa0858360:intron	30.0%
!!	TGACTCCAAATACTTGTTTT+TGG	-	chr5_4:55726769-55726788	None:intergenic	30.0%
!	AGCATAGAAACAACAAGTTT+TGG	+	chr5_4:55726807-55726826	Msa0858360:CDS	30.0%
	TTGTTGAGGGAATTAGAAAA+AGG	-	chr5_4:55726951-55726970	None:intergenic	30.0%
!!	TCTAATACTGACTTTGTTGA+GGG	-	chr5_4:55726964-55726983	None:intergenic	30.0%
	TTGTTCTCGCAAATACATAA+TGG	+	chr5_4:55727012-55727031	Msa0858360:intron	30.0%
!	TTCAATGTATACCATCGTTT+TGG	-	chr5_4:55727144-55727163	None:intergenic	30.0%
	AACGATGGTATACATTGAAA+TGG	+	chr5_4:55727145-55727164	Msa0858360:intron	30.0%
!	TGAAATGGACTTTAAATAGG+AGG	+	chr5_4:55727184-55727203	Msa0858360:intron	30.0%
	TTCTAGATCAACTTCCATTA+AGG	-	chr5_4:55727337-55727356	None:intergenic	30.0%
	ACTTGACTTCAAAGAGTAAA+CGG	-	chr5_4:55727444-55727463	None:intergenic	30.0%
	GTTTGAATAGAGTATGGATC+AGG	+	chr5_4:55724075-55724094	Msa0858360:intron	35.0%
	TCTCTGAGTAGGAAATTCTT+CGG	-	chr5_4:55724361-55724380	None:intergenic	35.0%
	TAAACAGAAATTACCGTGCT+AGG	+	chr5_4:55724419-55724438	Msa0858360:intron	35.0%
	AGTAGTAATAGTTCCTAGCA+CGG	-	chr5_4:55724435-55724454	None:intergenic	35.0%
	ACAAGATTTGTCCATCATCA+AGG	-	chr5_4:55724482-55724501	None:intergenic	35.0%
	TCAATTCCTATCTGTGTAGT+TGG	+	chr5_4:55724543-55724562	Msa0858360:intron	35.0%
	ATGTAACCAACTACACAGAT+AGG	-	chr5_4:55724552-55724571	None:intergenic	35.0%
	GAAATGCCGTTAATTATGCA+AGG	+	chr5_4:55724598-55724617	Msa0858360:intron	35.0%
	TGAATATCAATCTTCCCTCA+AGG	+	chr5_4:55724764-55724783	Msa0858360:intron	35.0%
!	CATTAGCTTATTTGCTTCTG+AGG	-	chr5_4:55724871-55724890	None:intergenic	35.0%
	CTCAGAAGCAAATAAGCTAA+TGG	+	chr5_4:55724869-55724888	Msa0858360:intron	35.0%
	GTAGCTGCTAAAGAAACAAT+GGG	+	chr5_4:55725265-55725284	Msa0858360:intron	35.0%
!	AGGAGACTTGGCATTTATTT+TGG	+	chr5_4:55725696-55725715	Msa0858360:intron	35.0%
!!	GATTGTGGTGGATATATTTG+TGG	-	chr5_4:55725722-55725741	None:intergenic	35.0%
!!!	ATCCGGATTTTCTGGAATAA+AGG	-	chr5_4:55725744-55725763	None:intergenic	35.0%
	TTTAACATGTATGCTTCGTG+AGG	+	chr5_4:55725781-55725800	Msa0858360:intron	35.0%
	TTGTTATCTAGAGGCTTAGA+GGG	-	chr5_4:55725919-55725938	None:intergenic	35.0%
	ACAAAGAAGTTTCATGAGCA+TGG	-	chr5_4:55726038-55726057	None:intergenic	35.0%
	AATTAGAGTGTATTTGGACG+GGG	+	chr5_4:55726277-55726296	Msa0858360:intron	35.0%
	GCTCACCAAAAACAAGTATT+TGG	+	chr5_4:55726761-55726780	Msa0858360:CDS	35.0%
	AGATCCTATTCAATGCAATG+TGG	+	chr5_4:55726919-55726938	Msa0858360:intron	35.0%
!!	CTCTAATACTGACTTTGTTG+AGG	-	chr5_4:55726965-55726984	None:intergenic	35.0%
	TCAGTATTAGAGTGTGCATT+AGG	+	chr5_4:55726973-55726992	Msa0858360:intron	35.0%
	CAGTATTAGAGTGTGCATTA+GGG	+	chr5_4:55726974-55726993	Msa0858360:intron	35.0%
	CGGATATCAACATCATCTAT+TGG	-	chr5_4:55727044-55727063	None:intergenic	35.0%
!!	TCTATTATCGGTGTTGCAAT+GGG	+	chr5_4:55727076-55727095	Msa0858360:intron	35.0%
	TTTGTGTGGTAACACCTTAA+TGG	+	chr5_4:55727320-55727339	Msa0858360:intron	35.0%
!!	AATCAACCGAACTTGCTAAT+AGG	+	chr5_4:55727479-55727498	Msa0858360:intron	35.0%
!!	ATCAACCGAACTTGCTAATA+GGG	+	chr5_4:55727480-55727499	Msa0858360:intron	35.0%
	TTTAGCCCTATTAGCAAGTT+CGG	-	chr5_4:55727488-55727507	None:intergenic	35.0%
	CTAATAGGGCTAAAAATGTG+TGG	+	chr5_4:55727494-55727513	Msa0858360:intron	35.0%
	TGTTGAAGATGATGAAGAAG+AGG	+	chr5_4:55727887-55727906	Msa0858360:CDS	35.0%
	AACCACAAACCAAGTTGCTT+GGG	-	chr5_4:55723989-55724008	None:intergenic	40.0%
	TAACCACAAACCAAGTTGCT+TGG	-	chr5_4:55723990-55724009	None:intergenic	40.0%
	TGAATAGAGTATGGATCAGG+TGG	+	chr5_4:55724078-55724097	Msa0858360:intron	40.0%
	CATAGCTATAGATATCTCCC+TGG	-	chr5_4:55724116-55724135	None:intergenic	40.0%
	CAGGGAGATATCTATAGCTA+TGG	+	chr5_4:55724114-55724133	Msa0858360:intron	40.0%
	CTTCTGTTAGAGATGCTTAC+TGG	+	chr5_4:55724141-55724160	Msa0858360:CDS	40.0%
	TGAAGATCAAACACGTGTTG+TGG	+	chr5_4:55724281-55724300	Msa0858360:CDS	40.0%
	TTTGTGAGCATTCTCTGAGT+AGG	-	chr5_4:55724372-55724391	None:intergenic	40.0%
	AGATTTGTCCATCATCAAGG+TGG	-	chr5_4:55724479-55724498	None:intergenic	40.0%
	GTCTTTCATTCATGGTCCAA+AGG	+	chr5_4:55725191-55725210	Msa0858360:intron	40.0%
	GGTAGCTGCTAAAGAAACAA+TGG	+	chr5_4:55725264-55725283	Msa0858360:intron	40.0%
	ATCTGTACTCCCTTTAGTCA+CGG	-	chr5_4:55725497-55725516	None:intergenic	40.0%
!	TCTGGAATAAAGGATTGTGG+TGG	-	chr5_4:55725734-55725753	None:intergenic	40.0%
!	GATGTCAAATCCGGATTTTC+TGG	-	chr5_4:55725752-55725771	None:intergenic	40.0%
	TAGTGGAAGGATGTCAAATC+CGG	-	chr5_4:55725761-55725780	None:intergenic	40.0%
	TGTTATCTAGAGGCTTAGAG+GGG	-	chr5_4:55725918-55725937	None:intergenic	40.0%
!	CTTGTTATCTAGAGGCTTAG+AGG	-	chr5_4:55725920-55725939	None:intergenic	40.0%
	AGAAGTTTCATGAGCATGGA+AGG	-	chr5_4:55726034-55726053	None:intergenic	40.0%
!	TAATCCGCACAAGAGTACAT+AGG	-	chr5_4:55726712-55726731	None:intergenic	40.0%
!	AAGTATTTGGAGTCACCATG+TGG	+	chr5_4:55726774-55726793	Msa0858360:CDS	40.0%
	TTTCTGCCAACTTGTGAGTA+AGG	-	chr5_4:55726878-55726897	None:intergenic	40.0%
	GTGTCCACATTGCATTGAAT+AGG	-	chr5_4:55726926-55726945	None:intergenic	40.0%
	AGATGATGTTGATATCCGTG+AGG	+	chr5_4:55727046-55727065	Msa0858360:intron	40.0%
!!	GTCTATTATCGGTGTTGCAA+TGG	+	chr5_4:55727075-55727094	Msa0858360:intron	40.0%
!	CATTGAAATGGACTCCAATG+CGG	+	chr5_4:55727157-55727176	Msa0858360:intron	40.0%
!!	CTCCAATGCGGATTTTGAAA+TGG	+	chr5_4:55727169-55727188	Msa0858360:intron	40.0%
	GTCCATTTCAAAATCCGCAT+TGG	-	chr5_4:55727174-55727193	None:intergenic	40.0%
	GGTTCACTTGAATGTGATAG+TGG	-	chr5_4:55727423-55727442	None:intergenic	40.0%
	AGAGTATGGATCAGGTGGTT+TGG	+	chr5_4:55724083-55724102	Msa0858360:intron	45.0%
	AATGGTGTCCACCTTGATGA+TGG	+	chr5_4:55724468-55724487	Msa0858360:intron	45.0%
!	TTCATCCTGAGTTTTCCACG+AGG	+	chr5_4:55725676-55725695	Msa0858360:intron	45.0%
	ATAAATGCCAAGTCTCCTCG+TGG	-	chr5_4:55725694-55725713	None:intergenic	45.0%
!	TTTGGACGGGGAATGTTTTG+AGG	+	chr5_4:55726289-55726308	Msa0858360:intron	45.0%
!!	TTGGACGGGGAATGTTTTGA+GGG	+	chr5_4:55726290-55726309	Msa0858360:intron	45.0%
	TTCTCAATTCTCTGACCTGC+TGG	+	chr5_4:55726736-55726755	Msa0858360:CDS	45.0%
	ATGCTTCCATAGTCTCCACA+TGG	-	chr5_4:55726792-55726811	None:intergenic	45.0%
	TTGTGAGTAAGGAGTCGACT+CGG	-	chr5_4:55726867-55726886	None:intergenic	45.0%
	TCGACTCCTTACTCACAAGT+TGG	+	chr5_4:55726869-55726888	Msa0858360:CDS	45.0%
!	CTCACAAGTTGGCAGAAACA+AGG	+	chr5_4:55726880-55726899	Msa0858360:CDS	45.0%
	ATAATAGACCCAGAGCCTCA+CGG	-	chr5_4:55727064-55727083	None:intergenic	45.0%
!!	TGAGGCTCTGGGTCTATTAT+CGG	+	chr5_4:55727064-55727083	Msa0858360:intron	45.0%
!!	ATCGGTGTTGCAATGGGTTA+AGG	+	chr5_4:55727082-55727101	Msa0858360:intron	45.0%
	CGAAATGGTCTCCAAAACGA+TGG	+	chr5_4:55727130-55727149	Msa0858360:intron	45.0%
!!	GTTCGGTTGATTGACTTGGA+AGG	-	chr5_4:55727471-55727490	None:intergenic	45.0%
	GCAAGTTCGGTTGATTGACT+TGG	-	chr5_4:55727475-55727494	None:intergenic	45.0%
!!!	TTAGAAATTTTTTAAAAATT+TGG	+	chr5_4:55725003-55725022	Msa0858360:intron	5.0%
!!!	TAGAAATTTTTTAAAAATTT+GGG	+	chr5_4:55725004-55725023	Msa0858360:intron	5.0%
!!!	AAATTTAAAATCAAATAAAA+AGG	-	chr5_4:55726120-55726139	None:intergenic	5.0%
!!!	TTTTATTTGATTTTATTTAA+TGG	+	chr5_4:55726153-55726172	Msa0858360:intron	5.0%
!!	TAAAATAATAATAATAATCA+TGG	+	chr5_4:55727639-55727658	Msa0858360:intron	5.0%
	TTCCTAGGAGGAACAACGCT+TGG	-	chr5_4:55723912-55723931	None:intergenic	50.0%
	TTCCTCCTAGGAATCGAACC+CGG	+	chr5_4:55723919-55723938	Msa0858360:CDS	50.0%
	GAATACCGGGTTCGATTCCT+AGG	-	chr5_4:55723927-55723946	None:intergenic	50.0%
!	AGCCCAAGCAACTTGGTTTG+TGG	+	chr5_4:55723984-55724003	Msa0858360:CDS	50.0%
!!	AGGTGGTTTGGTATCACTCC+AGG	+	chr5_4:55724095-55724114	Msa0858360:intron	50.0%
!!	GGTGGTTTGGTATCACTCCA+GGG	+	chr5_4:55724096-55724115	Msa0858360:intron	50.0%
	TCCAAAGGTTTCCCCTACTG+CGG	+	chr5_4:55725206-55725225	Msa0858360:intron	50.0%
	AGTCTCCTCGTGGAAAACTC+AGG	-	chr5_4:55725684-55725703	None:intergenic	50.0%
!	GAGTTTTCCACGAGGAGACT+TGG	+	chr5_4:55725684-55725703	Msa0858360:intron	50.0%
!	TGTATGCTTCGTGAGGTGCT+CGG	+	chr5_4:55725788-55725807	Msa0858360:intron	50.0%
	GCAGCCTATGTACTCTTGTG+CGG	+	chr5_4:55726705-55726724	Msa0858360:CDS	50.0%
!!!	GTTTTTGGTGAGCATCCAGC+AGG	-	chr5_4:55726754-55726773	None:intergenic	50.0%
	GAGTCACCATGTGGAGACTA+TGG	+	chr5_4:55726783-55726802	Msa0858360:CDS	50.0%
	TGTTGATATCCGTGAGGCTC+TGG	+	chr5_4:55727052-55727071	Msa0858360:intron	50.0%
	GTTGATATCCGTGAGGCTCT+GGG	+	chr5_4:55727053-55727072	Msa0858360:intron	50.0%
!	ATGCGTAGAGGCATGCACAC+TGG	+	chr5_4:55723886-55723905	Msa0858360:CDS	55.0%
	TACCGGGTTCGATTCCTAGG+AGG	-	chr5_4:55723924-55723943	None:intergenic	55.0%
	GAATCGAACCCGGTATTCCC+CGG	+	chr5_4:55723929-55723948	Msa0858360:CDS	55.0%
	AATCGAACCCGGTATTCCCC+GGG	+	chr5_4:55723930-55723949	Msa0858360:CDS	55.0%
	TCCGCAGTAGGGGAAACCTT+TGG	-	chr5_4:55725210-55725229	None:intergenic	55.0%
	CACAGTGCAATCCGCAGTAG+GGG	-	chr5_4:55725220-55725239	None:intergenic	55.0%
	CCACAGTGCAATCCGCAGTA+GGG	-	chr5_4:55725221-55725240	None:intergenic	55.0%
	ACCACAGTGCAATCCGCAGT+AGG	-	chr5_4:55725222-55725241	None:intergenic	55.0%
!	GTATGCTTCGTGAGGTGCTC+GGG	+	chr5_4:55725789-55725808	Msa0858360:intron	55.0%
	AGTGTGCATTAGGGACGACG+AGG	+	chr5_4:55726983-55727002	Msa0858360:intron	55.0%
!	GGCCAAGCGTTGTTCCTCCT+AGG	+	chr5_4:55723907-55723926	Msa0858360:CDS	60.0%
	AGGACGTACCCGGGGAATAC+CGG	-	chr5_4:55723941-55723960	None:intergenic	60.0%
	GTATTCCCCGGGTACGTCCT+TGG	+	chr5_4:55723941-55723960	Msa0858360:CDS	60.0%
	TCCTTCCAAGGACGTACCCG+GGG	-	chr5_4:55723949-55723968	None:intergenic	60.0%
	CTCCTTCCAAGGACGTACCC+GGG	-	chr5_4:55723950-55723969	None:intergenic	60.0%
	GCTCCTTCCAAGGACGTACC+CGG	-	chr5_4:55723951-55723970	None:intergenic	60.0%
	GTGGCAAGGAGCTCCTTCCA+AGG	-	chr5_4:55723961-55723980	None:intergenic	60.0%
	TGGAAGGAGCTCCTTGCCAC+TGG	+	chr5_4:55723961-55723980	Msa0858360:CDS	60.0%
	TTGCTTGGGCTCCAGTGGCA+AGG	-	chr5_4:55723975-55723994	None:intergenic	60.0%
	CCAAGTTGCTTGGGCTCCAG+TGG	-	chr5_4:55723980-55723999	None:intergenic	60.0%
	CCACTGGAGCCCAAGCAACT+TGG	+	chr5_4:55723977-55723996	Msa0858360:CDS	60.0%
!	CCCTACTGCGGATTGCACTG+TGG	+	chr5_4:55725218-55725237	Msa0858360:intron	60.0%
	GGACGTACCCGGGGAATACC+GGG	-	chr5_4:55723940-55723959	None:intergenic	65.0%
	TCCCCGGGTACGTCCTTGGA+AGG	+	chr5_4:55723945-55723964	Msa0858360:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	55723886	55727915	55723886	ID=Msa0858360;Name=Msa0858360
chr5_4	mRNA	55723886	55727915	55723886	ID=Msa0858360-mRNA-1;Parent=Msa0858360;Name=Msa0858360-mRNA-1;_AED=0.85;_eAED=0.85;_QI=0\|0\|0\|0.25\|1\|1\|4\|0\|232
chr5_4	exon	55723886	55724023	55723886	ID=Msa0858360-mRNA-1:exon:17064;Parent=Msa0858360-mRNA-1
chr5_4	exon	55724117	55724433	55724117	ID=Msa0858360-mRNA-1:exon:17065;Parent=Msa0858360-mRNA-1
chr5_4	exon	55726700	55726901	55726700	ID=Msa0858360-mRNA-1:exon:17066;Parent=Msa0858360-mRNA-1
chr5_4	exon	55727874	55727915	55727874	ID=Msa0858360-mRNA-1:exon:17067;Parent=Msa0858360-mRNA-1
chr5_4	CDS	55723886	55724023	55723886	ID=Msa0858360-mRNA-1:cds;Parent=Msa0858360-mRNA-1
chr5_4	CDS	55724117	55724433	55724117	ID=Msa0858360-mRNA-1:cds;Parent=Msa0858360-mRNA-1
chr5_4	CDS	55726700	55726901	55726700	ID=Msa0858360-mRNA-1:cds;Parent=Msa0858360-mRNA-1
chr5_4	CDS	55727874	55727915	55727874	ID=Msa0858360-mRNA-1:cds;Parent=Msa0858360-mRNA-1

Gene Sequence

>Msa0858360

ATGCGTAGAGGCATGCACACTGGCCAAGCGTTGTTCCTCCTAGGAATCGAACCCGGTATTCCCCGGGTACGTCCTTGGAAGGAGCTCCTTGCCACTGGAGCCCAAGCAACTTGGTTTGTGGTTAATGAATTAGTCAAAGGAGATATCTATAGCTATGGAATTCTTCTGTTAGAGATGCTTACTGGAAAGAGACCAACAGATAACACTTTTTGTGAGAATCTAAGCTTACACAAATTTTGTAAGATGAAAATTCCAGAAGGAATTCTTGATATAGTGGATTCATGCTTGCTCATGTCATTTGCTGAAGATCAAACACGTGTTGTGGAAAACAACATAAAAGAGTGTCTAGTGATGTTTGCTAAAATAGGAGTTGCATGTTCCGAAGAATTTCCTACTCAGAGAATGCTCACAAAAGATGTTATAATGAAGTTGCTTGAAATTAAACAGAAATTACCGCAATGCAGCCTATGTACTCTTGTGCGGATTATATATTCTCAATTCTCTGACCTGCTGGATGCTCACCAAAAACAAGTATTTGGAGTCACCATGTGGAGACTATGGAAGCATAGAAACAACAAGTTTTGGAATGATGCAACTGAAACAGTTAAGACATTTGTGACCGAGTCGACTCCTTACTCACAAGTTGGCAGAAACAAGTTTGATGAAGATGTGTTGAAGATGATGAAGAAGAGGAAATGA

Protein sequence

>Msa0858360

MRRGMHTGQALFLLGIEPGIPRVRPWKELLATGAQATWFVVNELVKGDIYSYGILLLEMLTGKRPTDNTFCENLSLHKFCKMKIPEGILDIVDSCLLMSFAEDQTRVVENNIKECLVMFAKIGVACSEEFPTQRMLTKDVIMKLLEIKQKLPQCSLCTLVRIIYSQFSDLLDAHQKQVFGVTMWRLWKHRNNKFWNDATETVKTFVTESTPYSQVGRNKFDEDVLKMMKKRK*