Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011870 | XP_013447886.1 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 1.76e-89 | 266 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011870 | A0A072TYJ6 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 8.40e-90 | 266 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0647300 | Msa1011870 | 0.801013 | 1.099858e-48 | -8.615850e-47 |
| Msa0686830 | Msa1011870 | 0.806009 | 1.012871e-49 | -8.615850e-47 |
| Msa0805390 | Msa1011870 | 0.807625 | 4.612435e-50 | -8.615850e-47 |
| Msa0219280 | Msa1011870 | 0.804159 | 2.469951e-49 | -8.615850e-47 |
| Msa1011800 | Msa1011870 | 0.936665 | 1.207753e-97 | -8.615850e-47 |
| Msa1011870 | Msa1053310 | 0.889037 | 3.466094e-73 | -8.615850e-47 |
| Msa1011870 | Msa1053340 | 0.893447 | 6.235359e-75 | -8.615850e-47 |
| Msa1011870 | Msa1095400 | 0.811227 | 7.778888e-51 | -8.615850e-47 |
| Msa1011870 | Msa1095410 | 0.846782 | 1.748985e-59 | -8.615850e-47 |
| Msa1011870 | Msa1142590 | 0.880262 | 6.343080e-70 | -8.615850e-47 |
| Msa1011870 | Msa1146980 | 0.814867 | 1.238257e-51 | -8.615850e-47 |
| Msa1011870 | Msa1165460 | 0.808700 | 2.722616e-50 | -8.615850e-47 |
| Msa1011870 | Msa1197720 | 0.840868 | 6.708517e-58 | -8.615850e-47 |
| Msa1011870 | Msa1251410 | 0.832957 | 7.027933e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011870 | MtrunA17_Chr7g0223391 | 94.891 | 137 | 7 | 0 | 1 | 137 | 18 | 154 | 4.05e-93 | 265 |
| Msa1011870 | MtrunA17_Chr7g0223401 | 94.161 | 137 | 8 | 0 | 1 | 137 | 6 | 142 | 6.42e-92 | 262 |
| Msa1011870 | MtrunA17_Chr8g0356181 | 47.857 | 140 | 70 | 1 | 1 | 137 | 1 | 140 | 1.12e-40 | 132 |
| Msa1011870 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 7.71e-20 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011870 | AT2G44310.1 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 1.29e-39 | 130 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_1:-16668049 | None:intergenic |
| CTTGAATGGCACAATTGATT+TGG | 0.268459 | 7_1:-16667794 | Msa1011870:CDS |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7_1:+16667847 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_1:+16668004 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_1:-16667713 | Msa1011870:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_1:+16667709 | None:intergenic |
| GAAGCATTTAATAGCTCAGT+TGG | 0.431408 | 7_1:-16667991 | Msa1011870:CDS |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_1:-16667984 | Msa1011870:CDS |
| CAAGGAGCTCCGGAGCTTGA+GGG | 0.440138 | 7_1:-16667908 | Msa1011870:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_1:+16668010 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_1:-16667719 | Msa1011870:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7_1:+16667848 | None:intergenic |
| CTCTTATGATGAGATGTTCA+AGG | 0.479132 | 7_1:-16667926 | Msa1011870:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7_1:-16667808 | Msa1011870:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_1:-16667740 | Msa1011870:CDS |
| TCAAGGAGCTCCGGAGCTTG+AGG | 0.525343 | 7_1:-16667909 | Msa1011870:CDS |
| ATGAGATGTTCAAGGAGCTC+CGG | 0.535600 | 7_1:-16667918 | Msa1011870:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7_1:-16667662 | Msa1011870:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_1:-16667739 | Msa1011870:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558215 | 7_1:-16667745 | Msa1011870:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_1:-16667878 | Msa1011870:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_1:+16668020 | None:intergenic |
| CGCCTCGACACAAACAGCGA+TGG | 0.598011 | 7_1:-16667955 | Msa1011870:CDS |
| AAAGCAGATGATGCTTGCAA+TGG | 0.609930 | 7_1:-16667752 | Msa1011870:CDS |
| GACCATCGCTGTTTGTGTCG+AGG | 0.621315 | 7_1:+16667953 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.625156 | 7_1:-16667744 | Msa1011870:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_1:16667654-16667673 | Msa1011870:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_1:16667698-16667717 | None:intergenic | 30.0% | |
| TTAATAGCTCAGTTGGAAAT+CGG | - | chr7_1:16667715-16667734 | Msa1011870:CDS | 30.0% | |
| ! | TGAAAGAGAGTACAATAAAG+TGG | - | chr7_1:16668037-16668056 | Msa1011870:CDS | 30.0% |
| ! | GAAGCATTTAATAGCTCAGT+TGG | - | chr7_1:16667708-16667727 | Msa1011870:CDS | 35.0% |
| CTCTTATGATGAGATGTTCA+AGG | - | chr7_1:16667773-16667792 | Msa1011870:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_1:16667810-16667829 | Msa1011870:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_1:16667821-16667840 | Msa1011870:CDS | 35.0% |
| CTTGAATGGCACAATTGATT+TGG | - | chr7_1:16667905-16667924 | Msa1011870:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_1:16667993-16668012 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTG+AGG | + | chr7_1:16668025-16668044 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_1:16667682-16667701 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_1:16667692-16667711 | None:intergenic | 40.0% | |
| TTTCAAAAACCCTCAAGCTC+CGG | + | chr7_1:16667803-16667822 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_1:16667891-16667910 | Msa1011870:CDS | 40.0% | |
| AAAGCAGATGATGCTTGCAA+TGG | - | chr7_1:16667947-16667966 | Msa1011870:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_1:16667980-16667999 | Msa1011870:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_1:16667986-16668005 | Msa1011870:CDS | 40.0% | |
| ATGAGATGTTCAAGGAGCTC+CGG | - | chr7_1:16667781-16667800 | Msa1011870:CDS | 45.0% | |
| AAACACGAGCAAGCTCGTTT+GGG | + | chr7_1:16667854-16667873 | None:intergenic | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | + | chr7_1:16667855-16667874 | None:intergenic | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_1:16667954-16667973 | Msa1011870:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | chr7_1:16667955-16667974 | Msa1011870:CDS | 45.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_1:16667960-16667979 | Msa1011870:CDS | 50.0% | |
| GACCATCGCTGTTTGTGTCG+AGG | + | chr7_1:16667749-16667768 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_1:16667959-16667978 | Msa1011870:CDS | 55.0% | |
| CGCCTCGACACAAACAGCGA+TGG | - | chr7_1:16667744-16667763 | Msa1011870:CDS | 60.0% | |
| TCAAGGAGCTCCGGAGCTTG+AGG | - | chr7_1:16667790-16667809 | Msa1011870:CDS | 60.0% | |
| CAAGGAGCTCCGGAGCTTGA+GGG | - | chr7_1:16667791-16667810 | Msa1011870:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 16667654 | 16668067 | 16667654 | ID=Msa1011870;Name=Msa1011870 |
| chr7_1 | mRNA | 16667654 | 16668067 | 16667654 | ID=Msa1011870-mRNA-1;Parent=Msa1011870;Name=Msa1011870-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_1 | exon | 16667654 | 16668067 | 16667654 | ID=Msa1011870-mRNA-1:exon:4236;Parent=Msa1011870-mRNA-1 |
| chr7_1 | CDS | 16667654 | 16668067 | 16667654 | ID=Msa1011870-mRNA-1:cds;Parent=Msa1011870-mRNA-1 |
| Gene Sequence |
| Protein sequence |
