Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095400 | RHN44804.1 | 93.431 | 137 | 9 | 0 | 1 | 137 | 6 | 142 | 1.88e-86 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095400 | A0A396GZB2 | 93.431 | 137 | 9 | 0 | 1 | 137 | 6 | 142 | 8.97e-87 | 258 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0647300 | Msa1095400 | 0.804862 | 1.762329e-49 | -8.615850e-47 |
| Msa0686830 | Msa1095400 | 0.805045 | 1.613691e-49 | -8.615850e-47 |
| Msa1011800 | Msa1095400 | 0.812884 | 3.385693e-51 | -8.615850e-47 |
| Msa1011870 | Msa1095400 | 0.811227 | 7.778888e-51 | -8.615850e-47 |
| Msa1030320 | Msa1095400 | 0.808621 | 2.830893e-50 | -8.615850e-47 |
| Msa1053310 | Msa1095400 | 0.849031 | 4.197047e-60 | -8.615850e-47 |
| Msa1053330 | Msa1095400 | 0.809473 | 1.859384e-50 | -8.615850e-47 |
| Msa1053340 | Msa1095400 | 0.830339 | 3.105859e-55 | -8.615850e-47 |
| Msa1095400 | Msa1095410 | 0.835640 | 1.491427e-56 | -8.615850e-47 |
| Msa1095400 | Msa1142580 | 0.806746 | 7.080714e-50 | -8.615850e-47 |
| Msa1095400 | Msa1142590 | 0.845002 | 5.326512e-59 | -8.615850e-47 |
| Msa1095400 | Msa1142600 | 0.852407 | 4.711096e-61 | -8.615850e-47 |
| Msa1095400 | Msa1157210 | 0.808788 | 2.607701e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095400 | MtrunA17_Chr7g0223401 | 93.431 | 137 | 9 | 0 | 1 | 137 | 6 | 142 | 1.73e-90 | 258 |
| Msa1095400 | MtrunA17_Chr7g0223391 | 91.971 | 137 | 11 | 0 | 1 | 137 | 18 | 154 | 1.02e-89 | 257 |
| Msa1095400 | MtrunA17_Chr8g0356181 | 48.571 | 140 | 69 | 1 | 1 | 137 | 1 | 140 | 7.71e-41 | 133 |
| Msa1095400 | MtrunA17_Chr1g0190921 | 37.405 | 131 | 77 | 2 | 1 | 131 | 1 | 126 | 4.72e-19 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095400 | AT2G44310.1 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 1.10e-38 | 128 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTCATCTTCAAGGAAA+TGG | 0.295262 | 7_3:-18247101 | None:intergenic |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7_3:-18247264 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.342383 | 7_3:-18247107 | None:intergenic |
| CTTGCAATGGCAGATGGGTT+GGG | 0.381320 | 7_3:+18247372 | Msa1095400:CDS |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_3:+18247398 | Msa1095400:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_3:-18247402 | None:intergenic |
| TTAATAGCTTGGTAGGTAAT+CGG | 0.402120 | 7_3:+18247127 | Msa1095400:CDS |
| CTTGAATGGCACAATTGATC+TGG | 0.437675 | 7_3:+18247317 | Msa1095400:CDS |
| GGGTTTCATGCCAATTCAAA+TGG | 0.441784 | 7_3:+18247392 | Msa1095400:CDS |
| TGAAGAAGCATTTAATAGCT+TGG | 0.442582 | 7_3:+18247116 | Msa1095400:CDS |
| CTCCTTCAACATCTCTTCAT+AGG | 0.458078 | 7_3:-18247187 | None:intergenic |
| GCTTGCAATGGCAGATGGGT+TGG | 0.461788 | 7_3:+18247371 | Msa1095400:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7_3:-18247263 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7_3:+18247203 | Msa1095400:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7_3:+18247303 | Msa1095400:CDS |
| CTCCTATGAAGAGATGTTGA+AGG | 0.486473 | 7_3:+18247185 | Msa1095400:CDS |
| GAAGCATTTAATAGCTTGGT+AGG | 0.494927 | 7_3:+18247120 | Msa1095400:CDS |
| TTTCGAAAACCCTCAAGCTC+CGG | 0.514432 | 7_3:-18247212 | None:intergenic |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7_3:+18247202 | Msa1095400:CDS |
| AATACACTTTGGTATTGATG+TGG | 0.523958 | 7_3:+18247233 | Msa1095400:CDS |
| AAGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7_3:+18247193 | Msa1095400:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7_3:+18247449 | Msa1095400:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558625 | 7_3:+18247366 | Msa1095400:CDS |
| GACCGTCGTTGTTTGTGTCG+AGG | 0.574792 | 7_3:-18247158 | None:intergenic |
| TTCAAGGAAATGGAAAATTG+TGG | 0.612346 | 7_3:-18247091 | None:intergenic |
| TAAGCAGATGATGCTTGCAA+TGG | 0.622742 | 7_3:+18247359 | Msa1095400:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.629974 | 7_3:+18247367 | Msa1095400:CDS |
| CGCCTCGACACAAACAACGA+CGG | 0.676658 | 7_3:+18247156 | Msa1095400:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGTGTAGAAATTTTAGA+TGG | + | chr7_3:18247066-18247085 | Msa1095400:CDS | 25.0% |
| !! | TGAGTGTAGAAATTTTAGAT+GGG | + | chr7_3:18247067-18247086 | Msa1095400:CDS | 25.0% |
| ! | GAGTGTAGAAATTTTAGATG+GGG | + | chr7_3:18247068-18247087 | Msa1095400:CDS | 30.0% |
| TTCAAGGAAATGGAAAATTG+TGG | - | chr7_3:18247094-18247113 | None:intergenic | 30.0% | |
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7_3:18247110-18247129 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+TGG | + | chr7_3:18247116-18247135 | Msa1095400:CDS | 30.0% |
| TTAATAGCTTGGTAGGTAAT+CGG | + | chr7_3:18247127-18247146 | Msa1095400:CDS | 30.0% | |
| !! | AATACACTTTGGTATTGATG+TGG | + | chr7_3:18247233-18247252 | Msa1095400:CDS | 30.0% |
| ! | TGAAAGAGAGTACAATAAAG+TGG | + | chr7_3:18247449-18247468 | Msa1095400:CDS | 30.0% |
| CTTCTTCATCTTCAAGGAAA+TGG | - | chr7_3:18247104-18247123 | None:intergenic | 35.0% | |
| ! | GAAGCATTTAATAGCTTGGT+AGG | + | chr7_3:18247120-18247139 | Msa1095400:CDS | 35.0% |
| ! | AGGGTTTTCGAAATACACTT+TGG | + | chr7_3:18247222-18247241 | Msa1095400:CDS | 35.0% |
| TCTTCCAAGACCATTTGAAT+TGG | - | chr7_3:18247405-18247424 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTG+AGG | - | chr7_3:18247437-18247456 | None:intergenic | 35.0% |
| CTCCTATGAAGAGATGTTGA+AGG | + | chr7_3:18247185-18247204 | Msa1095400:CDS | 40.0% | |
| CTCCTTCAACATCTCTTCAT+AGG | - | chr7_3:18247190-18247209 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | + | chr7_3:18247303-18247322 | Msa1095400:CDS | 40.0% | |
| CTTGAATGGCACAATTGATC+TGG | + | chr7_3:18247317-18247336 | Msa1095400:CDS | 40.0% | |
| TAAGCAGATGATGCTTGCAA+TGG | + | chr7_3:18247359-18247378 | Msa1095400:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7_3:18247392-18247411 | Msa1095400:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | + | chr7_3:18247398-18247417 | Msa1095400:CDS | 40.0% | |
| AAGAGATGTTGAAGGAGCTC+CGG | + | chr7_3:18247193-18247212 | Msa1095400:CDS | 45.0% | |
| TTTCGAAAACCCTCAAGCTC+CGG | - | chr7_3:18247215-18247234 | None:intergenic | 45.0% | |
| AAACACGAGCAAGCTCGTTT+GGG | - | chr7_3:18247266-18247285 | None:intergenic | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | - | chr7_3:18247267-18247286 | None:intergenic | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7_3:18247366-18247385 | Msa1095400:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | + | chr7_3:18247367-18247386 | Msa1095400:CDS | 45.0% | |
| ! | CTTGCAATGGCAGATGGGTT+GGG | + | chr7_3:18247372-18247391 | Msa1095400:CDS | 50.0% |
| CGCCTCGACACAAACAACGA+CGG | + | chr7_3:18247156-18247175 | Msa1095400:CDS | 55.0% | |
| GACCGTCGTTGTTTGTGTCG+AGG | - | chr7_3:18247161-18247180 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGT+TGG | + | chr7_3:18247371-18247390 | Msa1095400:CDS | 55.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | + | chr7_3:18247202-18247221 | Msa1095400:CDS | 60.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | + | chr7_3:18247203-18247222 | Msa1095400:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 18247066 | 18247479 | 18247066 | ID=Msa1095400;Name=Msa1095400 |
| chr7_3 | mRNA | 18247066 | 18247479 | 18247066 | ID=Msa1095400-mRNA-1;Parent=Msa1095400;Name=Msa1095400-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_3 | exon | 18247066 | 18247479 | 18247066 | ID=Msa1095400-mRNA-1:exon:3961;Parent=Msa1095400-mRNA-1 |
| chr7_3 | CDS | 18247066 | 18247479 | 18247066 | ID=Msa1095400-mRNA-1:cds;Parent=Msa1095400-mRNA-1 |
| Gene Sequence |
| Protein sequence |
