Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1028330 | XP_039683565.1 | 95.504 | 823 | 16 | 1 | 1 | 823 | 1 | 802 | 0.0 | 1619 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1028330 | sp|A6QLK4|TMM8B_BOVIN | 25.735 | 408 | 257 | 14 | 417 | 810 | 65 | 440 | 1.60e-16 | 87.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1028330 | A0A396H0V0 | 95.504 | 823 | 16 | 1 | 1 | 823 | 1 | 802 | 0.0 | 1619 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0115000 | Msa1028330 | 0.804576 | 2.021944e-49 | -8.615850e-47 |
| Msa0165210 | Msa1028330 | 0.800758 | 1.240345e-48 | -8.615850e-47 |
| Msa0272730 | Msa1028330 | 0.810749 | 9.874149e-51 | -8.615850e-47 |
| Msa0344590 | Msa1028330 | 0.806789 | 6.934869e-50 | -8.615850e-47 |
| Msa0431060 | Msa1028330 | 0.812054 | 5.142482e-51 | -8.615850e-47 |
| Msa0626280 | Msa1028330 | 0.833694 | 4.603918e-56 | -8.615850e-47 |
| Msa0626410 | Msa1028330 | 0.813619 | 2.335083e-51 | -8.615850e-47 |
| Msa0633700 | Msa1028330 | 0.806083 | 9.771887e-50 | -8.615850e-47 |
| Msa0658570 | Msa1028330 | 0.804331 | 2.274211e-49 | -8.615850e-47 |
| Msa0664570 | Msa1028330 | 0.815243 | 1.021644e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1028330 | MtrunA17_Chr7g0248071 | 95.504 | 823 | 16 | 1 | 1 | 823 | 1 | 802 | 0.0 | 1619 |
| Msa1028330 | MtrunA17_Chr8g0348621 | 68.260 | 816 | 227 | 6 | 14 | 821 | 31 | 822 | 0.0 | 1156 |
| Msa1028330 | MtrunA17_Chr2g0310481 | 86.538 | 52 | 6 | 1 | 1 | 52 | 1 | 51 | 4.40e-21 | 87.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1028330 | AT2G46060.1 | 50.905 | 829 | 330 | 15 | 21 | 822 | 29 | 807 | 0.0 | 841 |
| Msa1028330 | AT2G46060.3 | 50.181 | 829 | 330 | 16 | 21 | 822 | 29 | 801 | 0.0 | 820 |
| Msa1028330 | AT2G46060.2 | 50.064 | 783 | 315 | 14 | 21 | 777 | 29 | 761 | 0.0 | 785 |
Find 215 sgRNAs with CRISPR-Local
Find 1635 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACATTGTTCCTCTCACG+GGG | 0.665519 | 7_1:-61043988 | Msa1028330:CDS |
| AAAACTTTCTGTGCTCACGA+AGG | 0.643826 | 7_1:-61044947 | Msa1028330:CDS |
| AAACTATGAACTGACCCATC+AGG | 0.514878 | 7_1:-61040979 | Msa1028330:CDS |
| AAAGAACCACCTTCTGCCGA+AGG | 0.593285 | 7_1:+61042369 | None:intergenic |
| AAAGGTTTCACCTTTACTGC+TGG | 0.422733 | 7_1:+61049090 | None:intergenic |
| AAAGTATCTCATATTACATA+AGG | 0.422050 | 7_1:-61047631 | Msa1028330:intron |
| AAAGTTATGTTTAAGGGATC+TGG | 0.378645 | 7_1:+61047315 | None:intergenic |
| AAATGTGTGGCTAACGGTAT+TGG | 0.445673 | 7_1:-61040817 | Msa1028330:three_prime_UTR |
| AAATTTACAGTCCCCGTGAG+AGG | 0.597033 | 7_1:+61043977 | None:intergenic |
| AACCGAGCATTGAAGCATGT+GGG | 0.636332 | 7_1:+61044619 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTTATATAGCGCAAATCAAA+GGG | + | chr7_1:61040609-61040628 | None:intergenic | 30.0% | |
| TCTTATATAGCGCAAATCAA+AGG | + | chr7_1:61040610-61040629 | None:intergenic | 30.0% | |
| ATAACCTACTTATCAATCTC+AGG | + | chr7_1:61040912-61040931 | None:intergenic | 30.0% | |
| AATTAGAGCAAAACTCTGTA+CGG | - | chr7_1:61041097-61041116 | Msa1028330:intron | 30.0% | |
| CTATACACTATGAGCTTATA+AGG | + | chr7_1:61041354-61041373 | None:intergenic | 30.0% | |
| ACATCACAGATTAGAGAATA+AGG | + | chr7_1:61041600-61041619 | None:intergenic | 30.0% | |
| GCAAGAAAGGAATAATAATC+AGG | - | chr7_1:61041665-61041684 | Msa1028330:intron | 30.0% | |
| TATACTTACAAGGTCTACAT+CGG | + | chr7_1:61041951-61041970 | None:intergenic | 30.0% | |
| AAGAATCGGATATACTTACA+AGG | + | chr7_1:61041961-61041980 | None:intergenic | 30.0% | |
| CAGAAAGATAAGATTCTGAT+TGG | + | chr7_1:61042174-61042193 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 61040342 | 61049314 | 61040342 | ID=Msa1028330;Name=Msa1028330 |
| chr7_1 | mRNA | 61040342 | 61049314 | 61040342 | ID=Msa1028330-mRNA-1;Parent=Msa1028330;Name=Msa1028330-mRNA-1;_AED=0.08;_eAED=0.08;_QI=141|0.91|0.92|1|1|1|13|614|823 |
| chr7_1 | exon | 61040342 | 61041094 | 61040342 | ID=Msa1028330-mRNA-1:exon:12823;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61041182 | 61041334 | 61041182 | ID=Msa1028330-mRNA-1:exon:12822;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61041758 | 61041886 | 61041758 | ID=Msa1028330-mRNA-1:exon:12821;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61042009 | 61042253 | 61042009 | ID=Msa1028330-mRNA-1:exon:12820;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61042379 | 61042458 | 61042379 | ID=Msa1028330-mRNA-1:exon:12819;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61043434 | 61043498 | 61043434 | ID=Msa1028330-mRNA-1:exon:12818;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61043945 | 61044483 | 61043945 | ID=Msa1028330-mRNA-1:exon:12817;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61044574 | 61045164 | 61044574 | ID=Msa1028330-mRNA-1:exon:12816;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61045880 | 61045948 | 61045880 | ID=Msa1028330-mRNA-1:exon:12815;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61046914 | 61046987 | 61046914 | ID=Msa1028330-mRNA-1:exon:12814;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61047295 | 61047412 | 61047295 | ID=Msa1028330-mRNA-1:exon:12813;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61047632 | 61047753 | 61047632 | ID=Msa1028330-mRNA-1:exon:12812;Parent=Msa1028330-mRNA-1 |
| chr7_1 | exon | 61049026 | 61049314 | 61049026 | ID=Msa1028330-mRNA-1:exon:12811;Parent=Msa1028330-mRNA-1 |
| chr7_1 | five_prime_UTR | 61049174 | 61049314 | 61049174 | ID=Msa1028330-mRNA-1:five_prime_utr;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61049026 | 61049173 | 61049026 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61047632 | 61047753 | 61047632 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61047295 | 61047412 | 61047295 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61046914 | 61046987 | 61046914 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61045880 | 61045948 | 61045880 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61044574 | 61045164 | 61044574 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61043945 | 61044483 | 61043945 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61043434 | 61043498 | 61043434 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61042379 | 61042458 | 61042379 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61042009 | 61042253 | 61042009 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61041758 | 61041886 | 61041758 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61041182 | 61041334 | 61041182 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | CDS | 61040956 | 61041094 | 61040956 | ID=Msa1028330-mRNA-1:cds;Parent=Msa1028330-mRNA-1 |
| chr7_1 | three_prime_UTR | 61040342 | 61040955 | 61040342 | ID=Msa1028330-mRNA-1:three_prime_utr;Parent=Msa1028330-mRNA-1 |
| Gene Sequence |
>Msa1028330 ATGAGTTTTTTCAATTTGATTCTCACTCTTCTTGTGCTTCTTCTCTCTGCAAATGGCGAATCCAGCAGTAAAGGTGAAACCTTTATTGTTTCCAGCTTCAGCTACCCTCATACTAGACTCAGACCCTTTGATTTGCGCTATATAAGAGTTGATTTGCCGCCATGGTTTTCTGCAATGTCTATATCACTAAACTCCGATGTAGACCTTGTAAGTATATCCGATTCTTCTACTTTGATATCTCTTGCTTTAAAGTATCTCATATTACATAAGGATGTGTCTAAAATTGAAAAGGTTCCAATGAGCACACTGCCAATAATATGCTTTAGAGATGGAGGTCCTCCACTGCCAGATCCCTTAAACATAACTTTGAAAGATTCAGCTGTCTCAGGGATAAATGGTTTAGATGTGGAGCAGTGCTTTCCTATGCAGAAAAACATCACTGTGAAACTGACAAACAATCAGATATCTCCAGGTGTTTGGTTCGTTGGTCTTTTCAATGGCATTGGACCTACAAGGACACAGTCGAAGATGATTGTCCGAGGCCCAGCGTACTCCTTTGGTGCCAATATAACCGTGGAAGCATGCACAAATTCAATGATGACAGGAAATTTCTGTAACAGTCCAGTTTATCCGTTCTCATGCACAACATCTGATGTCAATAATGCTTTGAAGGCTACAGAAAACAACACAATGTTGGAAAATTTGATGACCTGCAAAAGTAATTTGAAAACTTTCTGTGCTCACGAAGGTGTACCAAACATCTTCTCCTTGGATATAATAAATGTGGCAGAAGAATTTACAGTTATGGCAAGTGATGTCAAATTCAGTGGTACAACTTCAAACAAAACAACTGGTGCAAATGATGTTGGTTTAATGTGTTTTGTTCGCCATGGTGCAATGCCTTCAGTGACTCTGTATGATTACTCCAGTGATTTAAATAAAGCACCGCTGGTTATTCATTCACCATTAATTGGTCGTTGGTACATTACTATCGTACCGATCAATCTTGTAAACACTCAGGATAGTAATGTGACGGCTTGCTATTCGGTGGAATCCCACATGCTTCAATGCTCGGTTGGGAAGGCTGGACCAAATTGTACAATGGATATCTACATGCTTCAGACATTTGTACGGAGAGGACCAACTCCCTTTGAATCATATTATTTACCGGTTGGTTCTGGAGCATCTTACACTTCTGCCAATTTCCCCATTGAAACGCTTTTGAGTAACTCATCATACAATGGAGAACCTGGTAACATTTGGACTTACTTACTTTTGGACATTCCTCGTGGTGCAGCCGGAGGAAATATTCATGTGCAACTATCCTCAGATGTGAAGATCAATTATGAAATTTATGCTAGATTTGGTGGATTGCCATCTCTTATTAGCTGGGACTATTATTATGCTAACAAGACAAGGAGAAGCGATACATCTTTGTTTTTCATGCTATATGATTCAAGTGATGGCAAGATCAATTTCTATATTATGTATGCTAGAGAAGGCACTTGGGGTTTTGGTCTTAGAAATCTTAATACCAGCAGAAGTGATCCTTCTAAAGGGCCAACTGTCATGTCTCTTTCACTTGAACGATGCCCAAAACATTGTTCCTCTCACGGGGACTGTAAATTTTCTTTTGATGCAAGTGGCTTGACATCATACAGCTTTTGCTCTTGCGATCGAAACCATGGAGGCATTGACTGTAGCGTTGAAATTGTAACACATCAAGGGCATGTACGACAATCATTTTTTCTCATTGTATCCAATGTTGCGGCCATACTTCCAGCCTATTGGGCCCTTCGGCAGAAGGCATTAGCAGAATGGGTTTTGTTCACATCCAGTGGAATTGCGAGTGGACTATATCATGCTTGTGACGTAGGTGCCTGGTGTGCATTGAACTATAATGTCTTACAGTTCATGGACTTCTGGCTCTCTTTCATGGCTATAATTAGCACTTTTCTTTATCTAGCTACTATTGATGAAGTATTGAAGAGGGCAATCCACACAGCTGTTGCTATCCTTACTGCCCTAATGGCTGTAACAAATGCAACTAGGTCTTCCAATGTTGTTCTTGTGATAGTGATTGGTGCGCTTGGTCTCCTAATTGGATGGGTTATAGAGTTGTCAACAAAGTATAGGTCCCTTTCCTTTTCAGTTAGATTCTCACTTGATTTCTCTCACTGTTTACAAGTGATAAAGCGATGGCTATGTAATCTTGTTGGGTCACTTCTAAGACGGTACCACTGGCCTTTTGCCTTAGCTGGTTTTTCTGCACTGTCAATGGCAATAGTAAGCTGGACACTTGAAACCAGTGAAACTTACTGGTTTTGGCATAGCTTTTGGCATATTTCAATATACACATCTTCTTTCTTCTTCCTTTGTTCGAAAGTAAATATTGTGGATAGTGAGAATCAGCTACCTGCAAATGCAAACTATGAACTGACCCATCAGGACTCACTTCCAAGAAGTGGTTAG |
| Protein sequence |
>Msa1028330 MSFFNLILTLLVLLLSANGESSSKGETFIVSSFSYPHTRLRPFDLRYIRVDLPPWFSAMSISLNSDVDLVSISDSSTLISLALKYLILHKDVSKIEKVPMSTLPIICFRDGGPPLPDPLNITLKDSAVSGINGLDVEQCFPMQKNITVKLTNNQISPGVWFVGLFNGIGPTRTQSKMIVRGPAYSFGANITVEACTNSMMTGNFCNSPVYPFSCTTSDVNNALKATENNTMLENLMTCKSNLKTFCAHEGVPNIFSLDIINVAEEFTVMASDVKFSGTTSNKTTGANDVGLMCFVRHGAMPSVTLYDYSSDLNKAPLVIHSPLIGRWYITIVPINLVNTQDSNVTACYSVESHMLQCSVGKAGPNCTMDIYMLQTFVRRGPTPFESYYLPVGSGASYTSANFPIETLLSNSSYNGEPGNIWTYLLLDIPRGAAGGNIHVQLSSDVKINYEIYARFGGLPSLISWDYYYANKTRRSDTSLFFMLYDSSDGKINFYIMYAREGTWGFGLRNLNTSRSDPSKGPTVMSLSLERCPKHCSSHGDCKFSFDASGLTSYSFCSCDRNHGGIDCSVEIVTHQGHVRQSFFLIVSNVAAILPAYWALRQKALAEWVLFTSSGIASGLYHACDVGAWCALNYNVLQFMDFWLSFMAIISTFLYLATIDEVLKRAIHTAVAILTALMAVTNATRSSNVVLVIVIGALGLLIGWVIELSTKYRSLSFSVRFSLDFSHCLQVIKRWLCNLVGSLLRRYHWPFALAGFSALSMAIVSWTLETSETYWFWHSFWHISIYTSSFFFLCSKVNIVDSENQLPANANYELTHQDSLPRSG* |
