AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0115000


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0115000 MtrunA17_Chr1g0161491 94.828 174 9 0 1 174 15 188 5.09e-112 343
Msa0115000 MtrunA17_Chr1g0161521 76.271 177 39 2 1 174 1 177 1.43e-83 267
Msa0115000 MtrunA17_Chr1g0161511 75.141 177 41 2 1 174 1 177 2.57e-82 263
Msa0115000 MtrunA17_Chr1g0161471 71.751 177 47 2 1 174 1 177 1.24e-79 256
Msa0115000 MtrunA17_Chr1g0161451 33.333 186 117 3 4 183 2 186 2.78e-27 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0115000 AT1G14790.1 49.123 171 85 2 5 174 3 172 2.78e-51 176

Find 34 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACGATGTTAAACTGTATTT+TGG 0.159141 1_3:+33509405 Msa0115000:CDS
GTGGATGCTAGTGTAGATTT+TGG 0.172622 1_3:+33509468 Msa0115000:CDS
AGCTTTCATATGAGAATATT+TGG 0.191630 1_3:+33509550 Msa0115000:CDS
GGAACTCCTATTTAAAAGTT+AGG 0.218706 1_3:+33509331 Msa0115000:CDS
TCCAAAGCCAAGGGCATTCT+TGG 0.267359 1_3:+33509374 Msa0115000:CDS
TGCAAAGTATCTATTGATTC+AGG 0.268018 1_3:+33509605 Msa0115000:CDS
AGGAAAGATTCTCTATATTA+TGG 0.268444 1_3:+33509442 Msa0115000:CDS
TCCAAGAATGCCCTTGGCTT+TGG 0.307615 1_3:-33509375 None:intergenic
AGTAAAACAATTGAGTTGTA+TGG 0.346819 1_3:+33509114 Msa0115000:CDS
TGAATAATTGCAAATGCTCT+TGG 0.351532 1_3:-33509243 None:intergenic
GCAGAACTAAATTGCGGTTC+GGG 0.394506 1_3:+33509310 Msa0115000:CDS
CTATTTAAAAGTTAGGGAAA+TGG 0.416320 1_3:+33509338 Msa0115000:CDS
AGCAGAACTAAATTGCGGTT+CGG 0.428037 1_3:+33509309 Msa0115000:CDS
ATATTTGTAGAGCATTATAC+TGG 0.432174 1_3:+33509171 Msa0115000:CDS
TGAGACACGTTCACAAAAGA+AGG 0.445945 1_3:-33509141 None:intergenic
GAACTCCTATTTAAAAGTTA+GGG 0.447970 1_3:+33509332 Msa0115000:CDS
TTGGGATTGGTATGAGAAAG+TGG 0.485612 1_3:+33509487 Msa0115000:CDS
CAATTTAGTTCTGCTAGGTA+AGG 0.518592 1_3:-33509301 None:intergenic
GATTGAGCTGCATCAACCAC+AGG 0.538892 1_3:+33509575 Msa0115000:CDS
AAACTGTCCAAGAATGCCCT+TGG 0.565377 1_3:-33509381 None:intergenic
GTAGAGCATTATACTGGTGA+AGG 0.567027 1_3:+33509177 Msa0115000:CDS
ATTCTCTATATTATGGAAGG+TGG 0.569959 1_3:+33509449 Msa0115000:CDS
AAGATTCTCTATATTATGGA+AGG 0.592994 1_3:+33509446 Msa0115000:CDS
AAAACATGCTACATTCATGA+TGG 0.594459 1_3:+33509278 Msa0115000:CDS
GTTGCTATGAAGATTAGAGT+AGG 0.605906 1_3:+33509207 Msa0115000:CDS
GAGATATTGATCCAAAGCCA+AGG 0.607190 1_3:+33509364 Msa0115000:CDS
TACCTAGCAGAACTAAATTG+CGG 0.623189 1_3:+33509304 Msa0115000:CDS
GAGACACGTTCACAAAAGAA+GGG 0.638551 1_3:-33509140 None:intergenic
AGATATTGATCCAAAGCCAA+GGG 0.640291 1_3:+33509365 Msa0115000:CDS
ATGAAGATTAGAGTAGGCAA+AGG 0.643746 1_3:+33509213 Msa0115000:CDS
AACCGCAATTTAGTTCTGCT+AGG 0.654522 1_3:-33509306 None:intergenic
ATTGAGCTGCATCAACCACA+GGG 0.682058 1_3:+33509576 Msa0115000:CDS
GGAAAGATTCAAGAATGACA+TGG 0.688166 1_3:+33509843 Msa0115000:intron
TTATACTGGTGAAGGAAGTG+TGG 0.711055 1_3:+33509185 Msa0115000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTAAAATATTTAATGAA+AGG - chr1_3:33509644-33509663 None:intergenic 10.0%
!!! TGTTTAAAATATTTAATGAA+AGG - chr1_3:33509644-33509663 None:intergenic 10.0%
!!! ATATCAAGAATGATTTTATC+AGG + chr1_3:33509822-33509841 Msa0115000:intron 20.0%
!!! ATATCAAGAATGATTTTATC+AGG + chr1_3:33509822-33509841 Msa0115000:intron 20.0%
!! AGTAAAACAATTGAGTTGTA+TGG + chr1_3:33509114-33509133 Msa0115000:CDS 25.0%
!! ATATTTGTAGAGCATTATAC+TGG + chr1_3:33509171-33509190 Msa0115000:CDS 25.0%
! GAACTCCTATTTAAAAGTTA+GGG + chr1_3:33509332-33509351 Msa0115000:CDS 25.0%
!! CATTTCCCTAACTTTTAAAT+AGG - chr1_3:33509340-33509359 None:intergenic 25.0%
! CTATTTAAAAGTTAGGGAAA+TGG + chr1_3:33509338-33509357 Msa0115000:CDS 25.0%
! AGGAAAGATTCTCTATATTA+TGG + chr1_3:33509442-33509461 Msa0115000:CDS 25.0%
! AAGATTCTCTATATTATGGA+AGG + chr1_3:33509446-33509465 Msa0115000:CDS 25.0%
!!! GTTTGAACTTCTTATTTTCA+TGG - chr1_3:33509527-33509546 None:intergenic 25.0%
! AGCTTTCATATGAGAATATT+TGG + chr1_3:33509550-33509569 Msa0115000:CDS 25.0%
! TCCATATGAATTATGAACAA+TGG + chr1_3:33509754-33509773 Msa0115000:intron 25.0%
! ACCATTGTTCATAATTCATA+TGG - chr1_3:33509758-33509777 None:intergenic 25.0%
! ATATGAATTATGAACAATGG+TGG + chr1_3:33509757-33509776 Msa0115000:intron 25.0%
!! AGTAAAACAATTGAGTTGTA+TGG + chr1_3:33509114-33509133 Msa0115000:CDS 25.0%
!! ATATTTGTAGAGCATTATAC+TGG + chr1_3:33509171-33509190 Msa0115000:CDS 25.0%
! GAACTCCTATTTAAAAGTTA+GGG + chr1_3:33509332-33509351 Msa0115000:CDS 25.0%
!! CATTTCCCTAACTTTTAAAT+AGG - chr1_3:33509340-33509359 None:intergenic 25.0%
! CTATTTAAAAGTTAGGGAAA+TGG + chr1_3:33509338-33509357 Msa0115000:CDS 25.0%
! AGGAAAGATTCTCTATATTA+TGG + chr1_3:33509442-33509461 Msa0115000:CDS 25.0%
! AAGATTCTCTATATTATGGA+AGG + chr1_3:33509446-33509465 Msa0115000:CDS 25.0%
!!! GTTTGAACTTCTTATTTTCA+TGG - chr1_3:33509527-33509546 None:intergenic 25.0%
! AGCTTTCATATGAGAATATT+TGG + chr1_3:33509550-33509569 Msa0115000:CDS 25.0%
! TCCATATGAATTATGAACAA+TGG + chr1_3:33509754-33509773 Msa0115000:intron 25.0%
! ACCATTGTTCATAATTCATA+TGG - chr1_3:33509758-33509777 None:intergenic 25.0%
! ATATGAATTATGAACAATGG+TGG + chr1_3:33509757-33509776 Msa0115000:intron 25.0%
TGAATAATTGCAAATGCTCT+TGG - chr1_3:33509246-33509265 None:intergenic 30.0%
AAAACATGCTACATTCATGA+TGG + chr1_3:33509278-33509297 Msa0115000:CDS 30.0%
GGAACTCCTATTTAAAAGTT+AGG + chr1_3:33509331-33509350 Msa0115000:CDS 30.0%
! GACGATGTTAAACTGTATTT+TGG + chr1_3:33509405-33509424 Msa0115000:CDS 30.0%
ATTCTCTATATTATGGAAGG+TGG + chr1_3:33509449-33509468 Msa0115000:CDS 30.0%
!! TGCAAAGTATCTATTGATTC+AGG + chr1_3:33509605-33509624 Msa0115000:CDS 30.0%
TGAATAATTGCAAATGCTCT+TGG - chr1_3:33509246-33509265 None:intergenic 30.0%
AAAACATGCTACATTCATGA+TGG + chr1_3:33509278-33509297 Msa0115000:CDS 30.0%
GGAACTCCTATTTAAAAGTT+AGG + chr1_3:33509331-33509350 Msa0115000:CDS 30.0%
! GACGATGTTAAACTGTATTT+TGG + chr1_3:33509405-33509424 Msa0115000:CDS 30.0%
ATTCTCTATATTATGGAAGG+TGG + chr1_3:33509449-33509468 Msa0115000:CDS 30.0%
!! TGCAAAGTATCTATTGATTC+AGG + chr1_3:33509605-33509624 Msa0115000:CDS 30.0%
GTTGCTATGAAGATTAGAGT+AGG + chr1_3:33509207-33509226 Msa0115000:CDS 35.0%
ATGAAGATTAGAGTAGGCAA+AGG + chr1_3:33509213-33509232 Msa0115000:CDS 35.0%
!! CAATTTAGTTCTGCTAGGTA+AGG - chr1_3:33509304-33509323 None:intergenic 35.0%
TACCTAGCAGAACTAAATTG+CGG + chr1_3:33509304-33509323 Msa0115000:CDS 35.0%
AGATATTGATCCAAAGCCAA+GGG + chr1_3:33509365-33509384 Msa0115000:CDS 35.0%
! TGGATGCTAGTGTAGATTTT+GGG + chr1_3:33509469-33509488 Msa0115000:CDS 35.0%
GGAAAGATTCAAGAATGACA+TGG + chr1_3:33509843-33509862 Msa0115000:intron 35.0%
GTTGCTATGAAGATTAGAGT+AGG + chr1_3:33509207-33509226 Msa0115000:CDS 35.0%
ATGAAGATTAGAGTAGGCAA+AGG + chr1_3:33509213-33509232 Msa0115000:CDS 35.0%
!! CAATTTAGTTCTGCTAGGTA+AGG - chr1_3:33509304-33509323 None:intergenic 35.0%
TACCTAGCAGAACTAAATTG+CGG + chr1_3:33509304-33509323 Msa0115000:CDS 35.0%
AGATATTGATCCAAAGCCAA+GGG + chr1_3:33509365-33509384 Msa0115000:CDS 35.0%
! TGGATGCTAGTGTAGATTTT+GGG + chr1_3:33509469-33509488 Msa0115000:CDS 35.0%
GGAAAGATTCAAGAATGACA+TGG + chr1_3:33509843-33509862 Msa0115000:intron 35.0%
GAGACACGTTCACAAAAGAA+GGG - chr1_3:33509143-33509162 None:intergenic 40.0%
TGAGACACGTTCACAAAAGA+AGG - chr1_3:33509144-33509163 None:intergenic 40.0%
! GTAGAGCATTATACTGGTGA+AGG + chr1_3:33509177-33509196 Msa0115000:CDS 40.0%
TTATACTGGTGAAGGAAGTG+TGG + chr1_3:33509185-33509204 Msa0115000:CDS 40.0%
!! AACCGCAATTTAGTTCTGCT+AGG - chr1_3:33509309-33509328 None:intergenic 40.0%
AGCAGAACTAAATTGCGGTT+CGG + chr1_3:33509309-33509328 Msa0115000:CDS 40.0%
GAGATATTGATCCAAAGCCA+AGG + chr1_3:33509364-33509383 Msa0115000:CDS 40.0%
!! TTTTGGCTGTCAGATCTCAA+AGG + chr1_3:33509422-33509441 Msa0115000:CDS 40.0%
! GTGGATGCTAGTGTAGATTT+TGG + chr1_3:33509468-33509487 Msa0115000:CDS 40.0%
!!! GCTAGTGTAGATTTTGGGAT+TGG + chr1_3:33509474-33509493 Msa0115000:CDS 40.0%
! TTGGGATTGGTATGAGAAAG+TGG + chr1_3:33509487-33509506 Msa0115000:CDS 40.0%
! TACTTTGCAGTTTTACCCTG+TGG - chr1_3:33509594-33509613 None:intergenic 40.0%
GAGACACGTTCACAAAAGAA+GGG - chr1_3:33509143-33509162 None:intergenic 40.0%
TGAGACACGTTCACAAAAGA+AGG - chr1_3:33509144-33509163 None:intergenic 40.0%
! GTAGAGCATTATACTGGTGA+AGG + chr1_3:33509177-33509196 Msa0115000:CDS 40.0%
TTATACTGGTGAAGGAAGTG+TGG + chr1_3:33509185-33509204 Msa0115000:CDS 40.0%
!! AACCGCAATTTAGTTCTGCT+AGG - chr1_3:33509309-33509328 None:intergenic 40.0%
AGCAGAACTAAATTGCGGTT+CGG + chr1_3:33509309-33509328 Msa0115000:CDS 40.0%
GAGATATTGATCCAAAGCCA+AGG + chr1_3:33509364-33509383 Msa0115000:CDS 40.0%
!! TTTTGGCTGTCAGATCTCAA+AGG + chr1_3:33509422-33509441 Msa0115000:CDS 40.0%
! GTGGATGCTAGTGTAGATTT+TGG + chr1_3:33509468-33509487 Msa0115000:CDS 40.0%
!!! GCTAGTGTAGATTTTGGGAT+TGG + chr1_3:33509474-33509493 Msa0115000:CDS 40.0%
! TTGGGATTGGTATGAGAAAG+TGG + chr1_3:33509487-33509506 Msa0115000:CDS 40.0%
! TACTTTGCAGTTTTACCCTG+TGG - chr1_3:33509594-33509613 None:intergenic 40.0%
GCAGAACTAAATTGCGGTTC+GGG + chr1_3:33509310-33509329 Msa0115000:CDS 45.0%
AAACTGTCCAAGAATGCCCT+TGG - chr1_3:33509384-33509403 None:intergenic 45.0%
ATTGAGCTGCATCAACCACA+GGG + chr1_3:33509576-33509595 Msa0115000:CDS 45.0%
GCAGAACTAAATTGCGGTTC+GGG + chr1_3:33509310-33509329 Msa0115000:CDS 45.0%
AAACTGTCCAAGAATGCCCT+TGG - chr1_3:33509384-33509403 None:intergenic 45.0%
ATTGAGCTGCATCAACCACA+GGG + chr1_3:33509576-33509595 Msa0115000:CDS 45.0%
!!! AACAAAATTTAAAATAAAAA+AGG - chr1_3:33509681-33509700 None:intergenic 5.0%
!!! TAATAGATATATATATTTTT+AGG + chr1_3:33509789-33509808 Msa0115000:intron 5.0%
!!! AACAAAATTTAAAATAAAAA+AGG - chr1_3:33509681-33509700 None:intergenic 5.0%
!!! TAATAGATATATATATTTTT+AGG + chr1_3:33509789-33509808 Msa0115000:intron 5.0%
!! TCCAAAGCCAAGGGCATTCT+TGG + chr1_3:33509374-33509393 Msa0115000:CDS 50.0%
TCCAAGAATGCCCTTGGCTT+TGG - chr1_3:33509378-33509397 None:intergenic 50.0%
GATTGAGCTGCATCAACCAC+AGG + chr1_3:33509575-33509594 Msa0115000:CDS 50.0%
!! TCCAAAGCCAAGGGCATTCT+TGG + chr1_3:33509374-33509393 Msa0115000:CDS 50.0%
TCCAAGAATGCCCTTGGCTT+TGG - chr1_3:33509378-33509397 None:intergenic 50.0%
GATTGAGCTGCATCAACCAC+AGG + chr1_3:33509575-33509594 Msa0115000:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 33509105 33509873 33509105 ID=Msa0115000;Name=Msa0115000
chr1_3 mRNA 33509105 33509873 33509105 ID=Msa0115000-mRNA-1;Parent=Msa0115000;Name=Msa0115000-mRNA-1;_AED=0.23;_eAED=0.27;_QI=0|0|0|1|1|1|2|0|183
chr1_3 exon 33509105 33509626 33509105 ID=Msa0115000-mRNA-1:exon:10507;Parent=Msa0115000-mRNA-1
chr1_3 exon 33509844 33509873 33509844 ID=Msa0115000-mRNA-1:exon:10508;Parent=Msa0115000-mRNA-1
chr1_3 CDS 33509105 33509626 33509105 ID=Msa0115000-mRNA-1:cds;Parent=Msa0115000-mRNA-1
chr1_3 CDS 33509844 33509873 33509844 ID=Msa0115000-mRNA-1:cds;Parent=Msa0115000-mRNA-1
Gene Sequence

>Msa0115000

ATGGGTAAAAGTAAAACAATTGAGTTGTATGGATTCCCTTCTTTTGTGAACGTGTCTCAAGTAAAGATATTTGTAGAGCATTATACTGGTGAAGGAAGTGTGGTTGCTATGAAGATTAGAGTAGGCAAAGGACAGAATCCAAGAGCATTTGCAATTATTCAATTCACTGCTGCAAAACATGCTACATTCATGATGGCCTTACCTAGCAGAACTAAATTGCGGTTCGGGAACTCCTATTTAAAAGTTAGGGAAATGGAAAGAGATATTGATCCAAAGCCAAGGGCATTCTTGGACAGTTTAGACGATGTTAAACTGTATTTTGGCTGTCAGATCTCAAAGGAAAGATTCTCTATATTATGGAAGGTGGATGCTAGTGTAGATTTTGGGATTGGTATGAGAAAGTGGCGATTCTCTATGCTCCATGAAAATAAGAAGTTCAAACTTGAGCTTTCATATGAGAATATTTGGAAGATTGAGCTGCATCAACCACAGGGTAAAACTGCAAAGTATCTATTGATTCAGGAAAGATTCAAGAATGACATGGCAGAATAA

Protein sequence

>Msa0115000

MGKSKTIELYGFPSFVNVSQVKIFVEHYTGEGSVVAMKIRVGKGQNPRAFAIIQFTAAKHATFMMALPSRTKLRFGNSYLKVREMERDIDPKPRAFLDSLDDVKLYFGCQISKERFSILWKVDASVDFGIGMRKWRFSMLHENKKFKLELSYENIWKIELHQPQGKTAKYLLIQERFKNDMAE*