Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053290 | XP_013447886.1 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 1.34e-88 | 264 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053290 | A0A072TYJ6 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 6.40e-89 | 264 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011790 | Msa1053290 | 0.860955 | 1.439301e-63 | -8.615850e-47 |
| Msa1011810 | Msa1053290 | 0.819656 | 1.035821e-52 | -8.615850e-47 |
| Msa1011820 | Msa1053290 | 0.855435 | 6.323512e-62 | -8.615850e-47 |
| Msa1011830 | Msa1053290 | 0.805020 | 1.632992e-49 | -8.615850e-47 |
| Msa1011840 | Msa1053290 | 0.847809 | 9.143364e-60 | -8.615850e-47 |
| Msa1011860 | Msa1053290 | 0.840753 | 7.190937e-58 | -8.615850e-47 |
| Msa1053290 | Msa1053330 | 0.840388 | 8.963223e-58 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053290 | MtrunA17_Chr7g0223391 | 94.891 | 137 | 7 | 0 | 1 | 137 | 18 | 154 | 2.27e-92 | 264 |
| Msa1053290 | MtrunA17_Chr7g0223401 | 89.781 | 137 | 14 | 0 | 1 | 137 | 6 | 142 | 2.52e-87 | 250 |
| Msa1053290 | MtrunA17_Chr8g0356181 | 47.857 | 140 | 70 | 1 | 1 | 137 | 1 | 140 | 2.95e-41 | 134 |
| Msa1053290 | MtrunA17_Chr1g0190921 | 35.878 | 131 | 79 | 1 | 1 | 131 | 1 | 126 | 3.80e-19 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053290 | AT2G44310.1 | 48.905 | 137 | 68 | 1 | 1 | 135 | 1 | 137 | 2.89e-41 | 134 |
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTTGATCTTGAAGAATTC+AGG | 0.278025 | 7_2:-22710148 | Msa1053290:CDS |
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_2:+22710212 | None:intergenic |
| TGAAAGAGAGTCCGGTAAAT+TGG | 0.350760 | 7_2:-22710027 | Msa1053290:CDS |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_2:+22710369 | None:intergenic |
| TCTTCCAACACCATTTGAAT+TGG | 0.382295 | 7_2:+22710074 | None:intergenic |
| CAGTTTGATCACAACTTGAA+TGG | 0.425360 | 7_2:-22710173 | Msa1053290:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_2:+22710375 | None:intergenic |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.454217 | 7_2:-22710349 | Msa1053290:CDS |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_2:+22710289 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_2:+22710213 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_2:-22710084 | Msa1053290:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_2:-22710291 | Msa1053290:CDS |
| CATGCCAATTCAAATGGTGT+TGG | 0.494072 | 7_2:-22710078 | Msa1053290:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.520375 | 7_2:-22710273 | Msa1053290:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_2:-22710105 | Msa1053290:CDS |
| ATAGCTGTTGAAAGAGAGTC+CGG | 0.521949 | 7_2:-22710035 | Msa1053290:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7_2:-22710356 | Msa1053290:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.545635 | 7_2:-22710110 | Msa1053290:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7_2:-22710360 | Msa1053290:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_2:-22710104 | Msa1053290:CDS |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.558625 | 7_2:-22710274 | Msa1053290:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_2:-22710243 | Msa1053290:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_2:+22710385 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_2:-22710117 | Msa1053290:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.619568 | 7_2:-22710109 | Msa1053290:CDS |
| TGATCACAACTTGAATGGCA+CGG | 0.635410 | 7_2:-22710168 | Msa1053290:CDS |
| TGATCTTGAAGAATTCAGGA+AGG | 0.643588 | 7_2:-22710144 | Msa1053290:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_2:-22710320 | Msa1053290:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGTGTAGAATTTTTGGA+TGG | - | chr7_2:22710019-22710038 | Msa1053290:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_2:22710063-22710082 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+CGG | - | chr7_2:22710069-22710088 | Msa1053290:CDS | 30.0% |
| TTAATAGCTCGGTAGGAAAT+CGG | - | chr7_2:22710080-22710099 | Msa1053290:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_2:22710175-22710194 | Msa1053290:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_2:22710186-22710205 | Msa1053290:CDS | 35.0% |
| CAGTTTGATCACAACTTGAA+TGG | - | chr7_2:22710256-22710275 | Msa1053290:CDS | 35.0% | |
| TGATCTTGAAGAATTCAGGA+AGG | - | chr7_2:22710285-22710304 | Msa1053290:CDS | 35.0% | |
| TAAGCAAATGATGCTTGCAA+TGG | - | chr7_2:22710312-22710331 | Msa1053290:CDS | 35.0% | |
| TCTTCCAACACCATTTGAAT+TGG | + | chr7_2:22710358-22710377 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTG+AGG | + | chr7_2:22710390-22710409 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_2:22710047-22710066 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_2:22710057-22710076 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | - | chr7_2:22710073-22710092 | Msa1053290:CDS | 40.0% |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_2:22710138-22710157 | Msa1053290:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_2:22710143-22710162 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_2:22710219-22710238 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_2:22710220-22710239 | None:intergenic | 40.0% | |
| TGATCACAACTTGAATGGCA+CGG | - | chr7_2:22710261-22710280 | Msa1053290:CDS | 40.0% | |
| CGGTTGATCTTGAAGAATTC+AGG | - | chr7_2:22710281-22710300 | Msa1053290:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_2:22710345-22710364 | Msa1053290:CDS | 40.0% | |
| CATGCCAATTCAAATGGTGT+TGG | - | chr7_2:22710351-22710370 | Msa1053290:CDS | 40.0% | |
| ATAGCTGTTGAAAGAGAGTC+CGG | - | chr7_2:22710394-22710413 | Msa1053290:CDS | 40.0% | |
| TGAAAGAGAGTCCGGTAAAT+TGG | - | chr7_2:22710402-22710421 | Msa1053290:CDS | 40.0% | |
| TGCCTCGACACAAATAACGA+CGG | - | chr7_2:22710109-22710128 | Msa1053290:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_2:22710319-22710338 | Msa1053290:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | chr7_2:22710320-22710339 | Msa1053290:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | + | chr7_2:22710114-22710133 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | - | chr7_2:22710155-22710174 | Msa1053290:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | - | chr7_2:22710156-22710175 | Msa1053290:CDS | 50.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_2:22710325-22710344 | Msa1053290:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_2:22710324-22710343 | Msa1053290:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 22710019 | 22710432 | 22710019 | ID=Msa1053290;Name=Msa1053290 |
| chr7_2 | mRNA | 22710019 | 22710432 | 22710019 | ID=Msa1053290-mRNA-1;Parent=Msa1053290;Name=Msa1053290-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_2 | exon | 22710019 | 22710432 | 22710019 | ID=Msa1053290-mRNA-1:exon:5152;Parent=Msa1053290-mRNA-1 |
| chr7_2 | CDS | 22710019 | 22710432 | 22710019 | ID=Msa1053290-mRNA-1:cds;Parent=Msa1053290-mRNA-1 |
| Gene Sequence |
| Protein sequence |
