Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011820 | XP_013447886.1 | 88.321 | 137 | 5 | 1 | 1 | 126 | 1 | 137 | 2.12e-78 | 237 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011820 | A0A072TYJ6 | 88.321 | 137 | 5 | 1 | 1 | 126 | 1 | 137 | 1.01e-78 | 237 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011790 | Msa1011820 | 0.877203 | 7.588394e-69 | -8.615850e-47 |
| Msa1011810 | Msa1011820 | 0.804009 | 2.652966e-49 | -8.615850e-47 |
| Msa1011820 | Msa1011840 | 0.987278 | 1.098028e-169 | -8.615850e-47 |
| Msa1011820 | Msa1011860 | 0.876353 | 1.494436e-68 | -8.615850e-47 |
| Msa1011820 | Msa1053290 | 0.855435 | 6.323512e-62 | -8.615850e-47 |
| Msa1011820 | Msa1053330 | 0.893179 | 7.997397e-75 | -8.615850e-47 |
| Msa1011820 | Msa1053340 | 0.825954 | 3.536197e-54 | -8.615850e-47 |
| Msa1011820 | Msa1095410 | 0.806569 | 7.717796e-50 | -8.615850e-47 |
| Msa1011820 | Msa1142590 | 0.826860 | 2.150869e-54 | -8.615850e-47 |
| Msa1011820 | Msa1142600 | 0.807629 | 4.603225e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011820 | MtrunA17_Chr7g0223391 | 88.321 | 137 | 5 | 1 | 1 | 126 | 18 | 154 | 3.63e-82 | 237 |
| Msa1011820 | MtrunA17_Chr7g0223401 | 83.212 | 137 | 12 | 1 | 1 | 126 | 6 | 142 | 4.00e-77 | 224 |
| Msa1011820 | MtrunA17_Chr8g0356181 | 43.571 | 140 | 65 | 1 | 1 | 126 | 1 | 140 | 1.35e-34 | 116 |
| Msa1011820 | MtrunA17_Chr1g0190921 | 31.452 | 124 | 80 | 1 | 1 | 124 | 1 | 119 | 4.90e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011820 | AT2G44310.1 | 45.255 | 137 | 62 | 1 | 1 | 124 | 1 | 137 | 1.60e-35 | 119 |
Find 26 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_1:-16609792 | None:intergenic |
| CATTTAAGCAGCCAATTTAC+TGG | 0.249329 | 7_1:+16609427 | None:intergenic |
| CGGTTGATCTTGAAGAATTC+AGG | 0.278025 | 7_1:-16609526 | Msa1011820:CDS |
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_1:+16609590 | None:intergenic |
| TGAAAGAGAGTCCAGTAAAT+TGG | 0.375239 | 7_1:-16609438 | Msa1011820:CDS |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_1:+16609747 | None:intergenic |
| CAGTTTGATCACAACTTGAA+TGG | 0.425360 | 7_1:-16609551 | Msa1011820:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_1:+16609753 | None:intergenic |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.454217 | 7_1:-16609727 | Msa1011820:CDS |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_1:+16609667 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_1:+16609591 | None:intergenic |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_1:-16609669 | Msa1011820:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.520375 | 7_1:-16609651 | Msa1011820:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_1:-16609483 | Msa1011820:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7_1:-16609734 | Msa1011820:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.545635 | 7_1:-16609488 | Msa1011820:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7_1:-16609738 | Msa1011820:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_1:-16609482 | Msa1011820:CDS |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.558625 | 7_1:-16609652 | Msa1011820:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_1:-16609621 | Msa1011820:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_1:+16609763 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_1:-16609495 | Msa1011820:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.619568 | 7_1:-16609487 | Msa1011820:CDS |
| TGATCACAACTTGAATGGCA+CGG | 0.635410 | 7_1:-16609546 | Msa1011820:CDS |
| TGATCTTGAAGAATTCAGGA+AGG | 0.643588 | 7_1:-16609522 | Msa1011820:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_1:-16609698 | Msa1011820:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_1:16609430-16609449 | Msa1011820:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_1:16609474-16609493 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+CGG | - | chr7_1:16609480-16609499 | Msa1011820:CDS | 30.0% |
| TTAATAGCTCGGTAGGAAAT+CGG | - | chr7_1:16609491-16609510 | Msa1011820:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_1:16609586-16609605 | Msa1011820:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_1:16609597-16609616 | Msa1011820:CDS | 35.0% |
| CAGTTTGATCACAACTTGAA+TGG | - | chr7_1:16609667-16609686 | Msa1011820:CDS | 35.0% | |
| TGATCTTGAAGAATTCAGGA+AGG | - | chr7_1:16609696-16609715 | Msa1011820:CDS | 35.0% | |
| TAAGCAAATGATGCTTGCAA+TGG | - | chr7_1:16609723-16609742 | Msa1011820:CDS | 35.0% | |
| TGAAAGAGAGTCCAGTAAAT+TGG | - | chr7_1:16609780-16609799 | Msa1011820:CDS | 35.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_1:16609458-16609477 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_1:16609468-16609487 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | - | chr7_1:16609484-16609503 | Msa1011820:CDS | 40.0% |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_1:16609549-16609568 | Msa1011820:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_1:16609554-16609573 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_1:16609630-16609649 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_1:16609631-16609650 | None:intergenic | 40.0% | |
| TGATCACAACTTGAATGGCA+CGG | - | chr7_1:16609672-16609691 | Msa1011820:CDS | 40.0% | |
| CGGTTGATCTTGAAGAATTC+AGG | - | chr7_1:16609692-16609711 | Msa1011820:CDS | 40.0% | |
| TGCCTCGACACAAATAACGA+CGG | - | chr7_1:16609520-16609539 | Msa1011820:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_1:16609730-16609749 | Msa1011820:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | chr7_1:16609731-16609750 | Msa1011820:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | + | chr7_1:16609525-16609544 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | - | chr7_1:16609566-16609585 | Msa1011820:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | - | chr7_1:16609567-16609586 | Msa1011820:CDS | 50.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_1:16609736-16609755 | Msa1011820:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_1:16609735-16609754 | Msa1011820:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 16609430 | 16609810 | 16609430 | ID=Msa1011820;Name=Msa1011820 |
| chr7_1 | mRNA | 16609430 | 16609810 | 16609430 | ID=Msa1011820-mRNA-1;Parent=Msa1011820;Name=Msa1011820-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|126 |
| chr7_1 | exon | 16609430 | 16609810 | 16609430 | ID=Msa1011820-mRNA-1:exon:4232;Parent=Msa1011820-mRNA-1 |
| chr7_1 | CDS | 16609430 | 16609810 | 16609430 | ID=Msa1011820-mRNA-1:cds;Parent=Msa1011820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
