Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011790 | XP_013447886.1 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 1.02e-88 | 264 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011790 | A0A072TYJ6 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 4.87e-89 | 264 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011790 | Msa1011810 | 0.824801 | 6.628159e-54 | -8.615850e-47 |
| Msa1011790 | Msa1011820 | 0.877203 | 7.588394e-69 | -8.615850e-47 |
| Msa1011790 | Msa1011830 | 0.822970 | 1.781094e-53 | -8.615850e-47 |
| Msa1011790 | Msa1011840 | 0.872654 | 2.692752e-67 | -8.615850e-47 |
| Msa1011790 | Msa1011860 | 0.906539 | 1.332447e-80 | -8.615850e-47 |
| Msa1011790 | Msa1053290 | 0.860955 | 1.439301e-63 | -8.615850e-47 |
| Msa1011790 | Msa1053330 | 0.828598 | 8.225064e-55 | -8.615850e-47 |
| Msa1011790 | Msa1095410 | 0.803356 | 3.624630e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011790 | MtrunA17_Chr7g0223391 | 94.891 | 137 | 7 | 0 | 1 | 137 | 18 | 154 | 1.29e-92 | 264 |
| Msa1011790 | MtrunA17_Chr7g0223401 | 89.781 | 137 | 14 | 0 | 1 | 137 | 6 | 142 | 1.54e-87 | 251 |
| Msa1011790 | MtrunA17_Chr8g0356181 | 49.286 | 140 | 68 | 1 | 1 | 137 | 1 | 140 | 1.24e-42 | 137 |
| Msa1011790 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 1.74e-20 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011790 | AT2G44310.1 | 48.905 | 137 | 68 | 1 | 1 | 135 | 1 | 137 | 1.38e-41 | 135 |
Find 29 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_1:-16585692 | None:intergenic |
| CGGTTGATCTTGAAGAATTC+AGG | 0.278025 | 7_1:-16585426 | Msa1011790:CDS |
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_1:+16585490 | None:intergenic |
| TGAAAGAGAGTCCGGTAAAT+TGG | 0.350760 | 7_1:-16585305 | Msa1011790:CDS |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_1:+16585647 | None:intergenic |
| TCTTCCAACACCATTTGAAT+TGG | 0.382295 | 7_1:+16585352 | None:intergenic |
| CAGTTTGATCACAACTTGAA+TGG | 0.425360 | 7_1:-16585451 | Msa1011790:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_1:+16585653 | None:intergenic |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.454217 | 7_1:-16585627 | Msa1011790:CDS |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_1:+16585567 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_1:+16585491 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_1:-16585362 | Msa1011790:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_1:-16585569 | Msa1011790:CDS |
| CATGCCAATTCAAATGGTGT+TGG | 0.494072 | 7_1:-16585356 | Msa1011790:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.520375 | 7_1:-16585551 | Msa1011790:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_1:-16585383 | Msa1011790:CDS |
| ATAGCTGTTGAAAGAGAGTC+CGG | 0.521949 | 7_1:-16585313 | Msa1011790:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7_1:-16585634 | Msa1011790:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.545635 | 7_1:-16585388 | Msa1011790:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7_1:-16585638 | Msa1011790:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_1:-16585382 | Msa1011790:CDS |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.558625 | 7_1:-16585552 | Msa1011790:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_1:-16585521 | Msa1011790:CDS |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_1:-16585395 | Msa1011790:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.610054 | 7_1:+16585663 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.619568 | 7_1:-16585387 | Msa1011790:CDS |
| TGATCACAACTTGAATGGCA+CGG | 0.635410 | 7_1:-16585446 | Msa1011790:CDS |
| TGATCTTGAAGAATTCAGGA+AGG | 0.643588 | 7_1:-16585422 | Msa1011790:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_1:-16585598 | Msa1011790:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_1:16585297-16585316 | Msa1011790:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_1:16585341-16585360 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+CGG | - | chr7_1:16585347-16585366 | Msa1011790:CDS | 30.0% |
| TTAATAGCTCGGTAGGAAAT+CGG | - | chr7_1:16585358-16585377 | Msa1011790:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_1:16585453-16585472 | Msa1011790:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_1:16585464-16585483 | Msa1011790:CDS | 35.0% |
| CAGTTTGATCACAACTTGAA+TGG | - | chr7_1:16585534-16585553 | Msa1011790:CDS | 35.0% | |
| TGATCTTGAAGAATTCAGGA+AGG | - | chr7_1:16585563-16585582 | Msa1011790:CDS | 35.0% | |
| TAAGCAAATGATGCTTGCAA+TGG | - | chr7_1:16585590-16585609 | Msa1011790:CDS | 35.0% | |
| TCTTCCAACACCATTTGAAT+TGG | + | chr7_1:16585636-16585655 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTG+AGG | + | chr7_1:16585668-16585687 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_1:16585325-16585344 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_1:16585335-16585354 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | - | chr7_1:16585351-16585370 | Msa1011790:CDS | 40.0% |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_1:16585416-16585435 | Msa1011790:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_1:16585421-16585440 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_1:16585497-16585516 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_1:16585498-16585517 | None:intergenic | 40.0% | |
| TGATCACAACTTGAATGGCA+CGG | - | chr7_1:16585539-16585558 | Msa1011790:CDS | 40.0% | |
| CGGTTGATCTTGAAGAATTC+AGG | - | chr7_1:16585559-16585578 | Msa1011790:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_1:16585623-16585642 | Msa1011790:CDS | 40.0% | |
| CATGCCAATTCAAATGGTGT+TGG | - | chr7_1:16585629-16585648 | Msa1011790:CDS | 40.0% | |
| ATAGCTGTTGAAAGAGAGTC+CGG | - | chr7_1:16585672-16585691 | Msa1011790:CDS | 40.0% | |
| TGAAAGAGAGTCCGGTAAAT+TGG | - | chr7_1:16585680-16585699 | Msa1011790:CDS | 40.0% | |
| TGCCTCGACACAAATAACGA+CGG | - | chr7_1:16585387-16585406 | Msa1011790:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_1:16585597-16585616 | Msa1011790:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | chr7_1:16585598-16585617 | Msa1011790:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | + | chr7_1:16585392-16585411 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | - | chr7_1:16585433-16585452 | Msa1011790:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | - | chr7_1:16585434-16585453 | Msa1011790:CDS | 50.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_1:16585603-16585622 | Msa1011790:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_1:16585602-16585621 | Msa1011790:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 16585297 | 16585710 | 16585297 | ID=Msa1011790;Name=Msa1011790 |
| chr7_1 | mRNA | 16585297 | 16585710 | 16585297 | ID=Msa1011790-mRNA-1;Parent=Msa1011790;Name=Msa1011790-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_1 | exon | 16585297 | 16585710 | 16585297 | ID=Msa1011790-mRNA-1:exon:4230;Parent=Msa1011790-mRNA-1 |
| chr7_1 | CDS | 16585297 | 16585710 | 16585297 | ID=Msa1011790-mRNA-1:cds;Parent=Msa1011790-mRNA-1 |
| Gene Sequence |
| Protein sequence |
