AlfalfaGEDB | Msa0468440

M. sativa cultivar zhongmu-4 / Msa0468440

KEGG: K02942
GO: GO:0001932 GO:0001934 GO:0002181 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009893 GO:0009987 GO:0010467 GO:0010562 GO:0010604 GO:0015934 GO:0016020 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022625 GO:0022626 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032991 GO:0033674 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042788 GO:0043043 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0098772 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0468440	XP_003602368.1	98.925	93	1	0	1	93	1	93	8.17e-57	181

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0468440	sp\|P52855\|RLA1_MAIZE	67.188	64	21	0	1	64	1	64	7.87e-26	95.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0468440	G7J8Y6	98.925	93	1	0	1	93	1	93	3.90e-57	181

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0128330	Msa0468440	0.801370	9.298350e-49	-8.615850e-47
Msa0174580	Msa0468440	0.812890	3.376101e-51	-8.615850e-47
Msa0191550	Msa0468440	0.802142	6.455710e-49	-8.615850e-47
Msa0194940	Msa0468440	0.805118	1.557697e-49	-8.615850e-47
Msa0217620	Msa0468440	0.817098	3.932928e-52	-8.615850e-47
Msa0225350	Msa0468440	0.829561	4.803900e-55	-8.615850e-47
Msa0419710	Msa0468440	0.825633	4.213937e-54	-8.615850e-47
Msa0450150	Msa0468440	0.826895	2.110129e-54	-8.615850e-47
Msa0468440	Msa0573550	0.802243	6.154775e-49	-8.615850e-47
Msa0468440	Msa0585570	0.822037	2.934001e-53	-8.615850e-47
Msa0468440	Msa0605890	0.819833	9.434298e-53	-8.615850e-47
Msa0468440	Msa0663400	0.822157	2.752475e-53	-8.615850e-47
Msa0468440	Msa0749000	0.813054	3.108330e-51	-8.615850e-47
Msa0468440	Msa0796490	0.813934	1.990450e-51	-8.615850e-47
Msa0468440	Msa0835190	0.823719	1.190596e-53	-8.615850e-47
Msa0468440	Msa0902730	0.847183	1.359032e-59	-8.615850e-47
Msa0468440	Msa1013430	0.815676	8.182876e-52	-8.615850e-47
Msa0468440	Msa1093500	0.821136	4.740685e-53	-8.615850e-47
Msa0468440	Msa1115510	0.823129	1.635059e-53	-8.615850e-47
Msa0468440	Msa1169180	0.812435	4.245116e-51	-8.615850e-47
Msa0468440	Msa1208330	0.855239	7.207709e-62	-8.615850e-47
Msa0468440	Msa1330730	0.880145	6.984557e-70	-8.615850e-47
Msa0468440	Msa1351020	0.815321	9.816432e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0468440	MtrunA17_Chr3g0127831	98.925	93	1	0	1	93	1	93	7.52e-61	181
Msa0468440	MtrunA17_Chr2g0311301	97.849	93	2	0	1	93	1	93	2.49e-60	180
Msa0468440	MtrunA17_Chr4g0013621	90.000	80	8	0	1	80	1	80	3.07e-38	124
Msa0468440	MtrunA17_Chr8g0345951	68.493	73	22	1	1	73	1	72	1.72e-29	102
Msa0468440	MtrunA17_Chr7g0276131	62.712	59	22	0	1	59	1	59	4.07e-21	80.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0468440	AT5G24510.1	58.889	90	32	2	1	86	1	89	1.82e-30	105
Msa0468440	AT5G47700.1	65.517	58	20	0	4	61	5	62	2.56e-23	87.0
Msa0468440	AT5G47700.2	65.517	58	20	0	4	61	5	62	2.56e-23	87.0
Msa0468440	AT1G01100.3	63.793	58	21	0	4	61	5	62	4.81e-23	85.9
Msa0468440	AT1G01100.4	63.793	58	21	0	4	61	5	62	8.01e-23	85.5
Msa0468440	AT1G01100.1	63.793	58	21	0	4	61	5	62	8.01e-23	85.5
Msa0468440	AT1G01100.2	63.793	58	21	0	4	61	5	62	8.01e-23	85.5
Msa0468440	AT4G00810.1	62.069	58	22	0	4	61	5	62	3.39e-22	84.0
Msa0468440	AT4G00810.2	62.069	58	22	0	4	61	5	62	3.39e-22	84.0

Find 49 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATATCGAAACTTCACATTT+TGG	0.211931	3_3:-70076482	Msa0468440:five_prime_UTR
CAAAATGAGTTCCGGCGAAT+TGG	0.294649	3_3:-70076408	Msa0468440:exon
AGAAACCTAGGTTAATTGAT+TGG	0.340250	3_3:+70076522	None:intergenic
ACGTCACCGTTGAATCTTAC+TGG	0.348075	3_3:-70076202	Msa0468440:CDS
AAAATGAGTTCCGGCGAATT+GGG	0.368280	3_3:-70076407	Msa0468440:CDS
CAGCAAATGACAGTATTTAT+CGG	0.372783	3_3:+70075688	None:intergenic
AGATGCAAGTTCTACAAAAT+AGG	0.379492	3_3:+70075751	None:intergenic
TGTTAAATGAATGCTAGTTC+TGG	0.390837	3_3:-70075899	Msa0468440:three_prime_UTR
TCTGAGCAAGCTTAGCGAAT+AGG	0.392998	3_3:+70076174	None:intergenic
GAAACCTAGGTTAATTGATT+GGG	0.394188	3_3:+70076523	None:intergenic
GATAATAGACTTACGGTGAT+TGG	0.399296	3_3:+70076344	None:intergenic
GCCGTATCTGCACCCGCTGC+TGG	0.401410	3_3:-70076095	Msa0468440:CDS
TCTGCACCCGCTGCTGGTGG+TGG	0.439771	3_3:-70076089	Msa0468440:CDS
ATTGTTCTGCCACACAAACT+AGG	0.448236	3_3:+70076440	None:intergenic
GCGGCTCCACCACCAGCAGC+GGG	0.451994	3_3:+70076083	None:intergenic
ATAATAGACTTACGGTGATT+GGG	0.452174	3_3:+70076345	None:intergenic
AATTGTATTGAAATTAGAAA+CGG	0.456874	3_3:-70075947	Msa0468440:three_prime_UTR
ATTGTATTGAAATTAGAAAC+GGG	0.459342	3_3:-70075946	Msa0468440:three_prime_UTR
GAAGATCGGTACTTTGTTGA+AGG	0.461021	3_3:-70076231	Msa0468440:CDS
ACCTGCCGCCGTTGAAAAGA+AGG	0.476631	3_3:-70076048	Msa0468440:CDS
TGCGGCTCCACCACCAGCAG+CGG	0.489051	3_3:+70076082	None:intergenic
TTGAACTCCGGCGCTGTTGG+TGG	0.510539	3_3:-70076128	Msa0468440:CDS
AAAGATATAATTTCCTCGGA+GGG	0.515109	3_3:-70075721	Msa0468440:three_prime_UTR
TGTAATGATGAGTTACTGAT+TGG	0.515616	3_3:-70075976	Msa0468440:three_prime_UTR
TGAAGTTTCGATATATATAG+TGG	0.520113	3_3:+70076490	None:intergenic
ACAATTCACAAAATGAGTTC+CGG	0.522809	3_3:-70076416	Msa0468440:exon
TATAAAAGATATAATTTCCT+CGG	0.524151	3_3:-70075725	Msa0468440:three_prime_UTR
AGCGGCACCACCAACAGCGC+CGG	0.527844	3_3:+70076121	None:intergenic
ACCAGCAGCGGGTGCAGATA+CGG	0.530473	3_3:+70076094	None:intergenic
TCCATCGTCGTGGAGGATCA+AGG	0.532980	3_3:+70076370	None:intergenic
GCAAGCTTAGCGAATAGGCT+TGG	0.542190	3_3:+70076179	None:intergenic
TTTGTTTAGGCGGAGAAGAT+CGG	0.543289	3_3:-70076245	Msa0468440:intron
GGCAGGTTCGGCAGCTGCTG+CGG	0.548908	3_3:+70076064	None:intergenic
TTGTTCTGCCACACAAACTA+GGG	0.550348	3_3:+70076441	None:intergenic
GTAAATGCAGCCCAATTCGC+CGG	0.571217	3_3:+70076397	None:intergenic
ACCTTGATCCTCCACGACGA+TGG	0.573503	3_3:-70076371	Msa0468440:CDS
AAGGCCCAATCAATTAACCT+AGG	0.578918	3_3:-70076527	Msa0468440:five_prime_UTR
TTCGTAAACCCTAGTTTGTG+TGG	0.589308	3_3:-70076449	Msa0468440:five_prime_UTR
GTATCTGCACCCGCTGCTGG+TGG	0.603091	3_3:-70076092	Msa0468440:CDS
TGGCGTGTTATCAGAAACCT+AGG	0.605084	3_3:+70076510	None:intergenic
GCTTGGCCAGTAAGATTCAA+CGG	0.629260	3_3:+70076196	None:intergenic
AAAAGATATAATTTCCTCGG+AGG	0.639485	3_3:-70075722	Msa0468440:three_prime_UTR
AAGATATAATTTCCTCGGAG+GGG	0.640697	3_3:-70075720	Msa0468440:three_prime_UTR
TTGGGATTCCATCGTCGTGG+AGG	0.650788	3_3:+70076363	None:intergenic
TGATTGGGATTCCATCGTCG+TGG	0.662981	3_3:+70076360	None:intergenic
TTTGTGCTGTGATCAAGTTG+GGG	0.663474	3_3:-70075779	Msa0468440:three_prime_UTR
GGGTGCAGATACGGCAACAG+CGG	0.665524	3_3:+70076103	None:intergenic
CGGATACAGAATCCCCTCCG+AGG	0.696429	3_3:+70075708	None:intergenic
ATCGTCGTGGAGGATCAAGG+TGG	0.743990	3_3:+70076373	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTATATGTTTTTATCTTA+AGG	+	chr3_3:70076363-70076382	None:intergenic	10.0%
!!!	AATTATATGTTTTTATCTTA+AGG	+	chr3_3:70076363-70076382	None:intergenic	10.0%
!!	AATTGTATTGAAATTAGAAA+CGG	-	chr3_3:70076264-70076283	Msa0468440:intron	15.0%
!!	TATAAAAGATATAATTTCCT+CGG	-	chr3_3:70076486-70076505	Msa0468440:five_prime_UTR	15.0%
!!	AATTGTATTGAAATTAGAAA+CGG	-	chr3_3:70076264-70076283	Msa0468440:intron	15.0%
!!	TATAAAAGATATAATTTCCT+CGG	-	chr3_3:70076486-70076505	Msa0468440:five_prime_UTR	15.0%
!!!	ATTTTTCTTTTGTTACTGTT+TGG	-	chr3_3:70075936-70075955	Msa0468440:three_prime_UTR	20.0%
!!!	TTTTTCTTTTGTTACTGTTT+GGG	-	chr3_3:70075937-70075956	Msa0468440:three_prime_UTR	20.0%
!!	ATTGTATTGAAATTAGAAAC+GGG	-	chr3_3:70076265-70076284	Msa0468440:intron	20.0%
!!!	ATTTTTCTTTTGTTACTGTT+TGG	-	chr3_3:70075936-70075955	Msa0468440:three_prime_UTR	20.0%
!!!	TTTTTCTTTTGTTACTGTTT+GGG	-	chr3_3:70075937-70075956	Msa0468440:three_prime_UTR	20.0%
!!	ATTGTATTGAAATTAGAAAC+GGG	-	chr3_3:70076265-70076284	Msa0468440:intron	20.0%
!	TGAAGTTTCGATATATATAG+TGG	+	chr3_3:70075724-70075743	None:intergenic	25.0%
!!	TATATCGAAACTTCACATTT+TGG	-	chr3_3:70075729-70075748	Msa0468440:three_prime_UTR	25.0%
!	TATAGAAATGGAATTGAAGA+GGG	+	chr3_3:70075892-70075911	None:intergenic	25.0%
!	TTATAGAAATGGAATTGAAG+AGG	+	chr3_3:70075893-70075912	None:intergenic	25.0%
!	CGATTTGAAGATTATAGAAA+TGG	+	chr3_3:70075904-70075923	None:intergenic	25.0%
!!	AAACAATACCTTCTTTTCAA+CGG	+	chr3_3:70076174-70076193	None:intergenic	25.0%
!	TGAAGTTTCGATATATATAG+TGG	+	chr3_3:70075724-70075743	None:intergenic	25.0%
!!	TATATCGAAACTTCACATTT+TGG	-	chr3_3:70075729-70075748	Msa0468440:three_prime_UTR	25.0%
!	TATAGAAATGGAATTGAAGA+GGG	+	chr3_3:70075892-70075911	None:intergenic	25.0%
!	TTATAGAAATGGAATTGAAG+AGG	+	chr3_3:70075893-70075912	None:intergenic	25.0%
!	CGATTTGAAGATTATAGAAA+TGG	+	chr3_3:70075904-70075923	None:intergenic	25.0%
!!	AAACAATACCTTCTTTTCAA+CGG	+	chr3_3:70076174-70076193	None:intergenic	25.0%
	GAAACCTAGGTTAATTGATT+GGG	+	chr3_3:70075691-70075710	None:intergenic	30.0%
	AGAAACCTAGGTTAATTGAT+TGG	+	chr3_3:70075692-70075711	None:intergenic	30.0%
	ACAATTCACAAAATGAGTTC+CGG	-	chr3_3:70075795-70075814	Msa0468440:three_prime_UTR	30.0%
	ATAATAGACTTACGGTGATT+GGG	+	chr3_3:70075869-70075888	None:intergenic	30.0%
	TGTAATGATGAGTTACTGAT+TGG	-	chr3_3:70076235-70076254	Msa0468440:CDS	30.0%
	TGTTAAATGAATGCTAGTTC+TGG	-	chr3_3:70076312-70076331	Msa0468440:intron	30.0%
	AGATGCAAGTTCTACAAAAT+AGG	+	chr3_3:70076463-70076482	None:intergenic	30.0%
!	ATTTTGTAGAACTTGCATCT+AGG	-	chr3_3:70076463-70076482	Msa0468440:five_prime_UTR	30.0%
	AAAAGATATAATTTCCTCGG+AGG	-	chr3_3:70076489-70076508	Msa0468440:five_prime_UTR	30.0%
	AAAGATATAATTTCCTCGGA+GGG	-	chr3_3:70076490-70076509	Msa0468440:five_prime_UTR	30.0%
	CAGCAAATGACAGTATTTAT+CGG	+	chr3_3:70076526-70076545	None:intergenic	30.0%
	GAAACCTAGGTTAATTGATT+GGG	+	chr3_3:70075691-70075710	None:intergenic	30.0%
	AGAAACCTAGGTTAATTGAT+TGG	+	chr3_3:70075692-70075711	None:intergenic	30.0%
	ACAATTCACAAAATGAGTTC+CGG	-	chr3_3:70075795-70075814	Msa0468440:three_prime_UTR	30.0%
	ATAATAGACTTACGGTGATT+GGG	+	chr3_3:70075869-70075888	None:intergenic	30.0%
	TGTAATGATGAGTTACTGAT+TGG	-	chr3_3:70076235-70076254	Msa0468440:CDS	30.0%
	TGTTAAATGAATGCTAGTTC+TGG	-	chr3_3:70076312-70076331	Msa0468440:intron	30.0%
	AGATGCAAGTTCTACAAAAT+AGG	+	chr3_3:70076463-70076482	None:intergenic	30.0%
!	ATTTTGTAGAACTTGCATCT+AGG	-	chr3_3:70076463-70076482	Msa0468440:five_prime_UTR	30.0%
	AAAAGATATAATTTCCTCGG+AGG	-	chr3_3:70076489-70076508	Msa0468440:five_prime_UTR	30.0%
	AAAGATATAATTTCCTCGGA+GGG	-	chr3_3:70076490-70076509	Msa0468440:five_prime_UTR	30.0%
	CAGCAAATGACAGTATTTAT+CGG	+	chr3_3:70076526-70076545	None:intergenic	30.0%
	GATAATAGACTTACGGTGAT+TGG	+	chr3_3:70075870-70075889	None:intergenic	35.0%
	GAAGAGGGATAATAGACTTA+CGG	+	chr3_3:70075877-70075896	None:intergenic	35.0%
!	TCTTTTGTTACTGTTTGGGT+TGG	-	chr3_3:70075941-70075960	Msa0468440:three_prime_UTR	35.0%
!!!	GTTTGGGTTGGTTTTTGTTT+AGG	-	chr3_3:70075953-70075972	Msa0468440:three_prime_UTR	35.0%
!	ATTTTGTGCTGTGATCAAGT+TGG	-	chr3_3:70076430-70076449	Msa0468440:five_prime_UTR	35.0%
!	TTTTGTGCTGTGATCAAGTT+GGG	-	chr3_3:70076431-70076450	Msa0468440:five_prime_UTR	35.0%
	AAGATATAATTTCCTCGGAG+GGG	-	chr3_3:70076491-70076510	Msa0468440:five_prime_UTR	35.0%
	GATAATAGACTTACGGTGAT+TGG	+	chr3_3:70075870-70075889	None:intergenic	35.0%
	GAAGAGGGATAATAGACTTA+CGG	+	chr3_3:70075877-70075896	None:intergenic	35.0%
!	TCTTTTGTTACTGTTTGGGT+TGG	-	chr3_3:70075941-70075960	Msa0468440:three_prime_UTR	35.0%
!!!	GTTTGGGTTGGTTTTTGTTT+AGG	-	chr3_3:70075953-70075972	Msa0468440:three_prime_UTR	35.0%
!	ATTTTGTGCTGTGATCAAGT+TGG	-	chr3_3:70076430-70076449	Msa0468440:five_prime_UTR	35.0%
!	TTTTGTGCTGTGATCAAGTT+GGG	-	chr3_3:70076431-70076450	Msa0468440:five_prime_UTR	35.0%
	AAGATATAATTTCCTCGGAG+GGG	-	chr3_3:70076491-70076510	Msa0468440:five_prime_UTR	35.0%
!!!	AACTTCACATTTTGGTGCTC+TGG	-	chr3_3:70075737-70075756	Msa0468440:three_prime_UTR	40.0%
!	TTCGTAAACCCTAGTTTGTG+TGG	-	chr3_3:70075762-70075781	Msa0468440:three_prime_UTR	40.0%
	TTGTTCTGCCACACAAACTA+GGG	+	chr3_3:70075773-70075792	None:intergenic	40.0%
	ATTGTTCTGCCACACAAACT+AGG	+	chr3_3:70075774-70075793	None:intergenic	40.0%
	AAAATGAGTTCCGGCGAATT+GGG	-	chr3_3:70075804-70075823	Msa0468440:three_prime_UTR	40.0%
!!!	TGGGTTGGTTTTTGTTTAGG+CGG	-	chr3_3:70075956-70075975	Msa0468440:three_prime_UTR	40.0%
	TTTGTTTAGGCGGAGAAGAT+CGG	-	chr3_3:70075966-70075985	Msa0468440:three_prime_UTR	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	chr3_3:70075980-70075999	Msa0468440:three_prime_UTR	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	chr3_3:70076071-70076090	Msa0468440:CDS	40.0%
!	CAATACCTTCTTTTCAACGG+CGG	+	chr3_3:70076171-70076190	None:intergenic	40.0%
!	TTTGTGCTGTGATCAAGTTG+GGG	-	chr3_3:70076432-70076451	Msa0468440:five_prime_UTR	40.0%
!!!	AACTTCACATTTTGGTGCTC+TGG	-	chr3_3:70075737-70075756	Msa0468440:three_prime_UTR	40.0%
!	TTCGTAAACCCTAGTTTGTG+TGG	-	chr3_3:70075762-70075781	Msa0468440:three_prime_UTR	40.0%
	TTGTTCTGCCACACAAACTA+GGG	+	chr3_3:70075773-70075792	None:intergenic	40.0%
	ATTGTTCTGCCACACAAACT+AGG	+	chr3_3:70075774-70075793	None:intergenic	40.0%
	AAAATGAGTTCCGGCGAATT+GGG	-	chr3_3:70075804-70075823	Msa0468440:three_prime_UTR	40.0%
!!!	TGGGTTGGTTTTTGTTTAGG+CGG	-	chr3_3:70075956-70075975	Msa0468440:three_prime_UTR	40.0%
	TTTGTTTAGGCGGAGAAGAT+CGG	-	chr3_3:70075966-70075985	Msa0468440:three_prime_UTR	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	chr3_3:70075980-70075999	Msa0468440:three_prime_UTR	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	chr3_3:70076071-70076090	Msa0468440:CDS	40.0%
!	CAATACCTTCTTTTCAACGG+CGG	+	chr3_3:70076171-70076190	None:intergenic	40.0%
!	TTTGTGCTGTGATCAAGTTG+GGG	-	chr3_3:70076432-70076451	Msa0468440:five_prime_UTR	40.0%
	TGGCGTGTTATCAGAAACCT+AGG	+	chr3_3:70075704-70075723	None:intergenic	45.0%
	CAAAATGAGTTCCGGCGAAT+TGG	-	chr3_3:70075803-70075822	Msa0468440:three_prime_UTR	45.0%
	ACGTCACCGTTGAATCTTAC+TGG	-	chr3_3:70076009-70076028	Msa0468440:CDS	45.0%
	GCTTGGCCAGTAAGATTCAA+CGG	+	chr3_3:70076018-70076037	None:intergenic	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	chr3_3:70076040-70076059	None:intergenic	45.0%
	TGGCGTGTTATCAGAAACCT+AGG	+	chr3_3:70075704-70075723	None:intergenic	45.0%
	CAAAATGAGTTCCGGCGAAT+TGG	-	chr3_3:70075803-70075822	Msa0468440:three_prime_UTR	45.0%
	ACGTCACCGTTGAATCTTAC+TGG	-	chr3_3:70076009-70076028	Msa0468440:CDS	45.0%
	GCTTGGCCAGTAAGATTCAA+CGG	+	chr3_3:70076018-70076037	None:intergenic	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	chr3_3:70076040-70076059	None:intergenic	45.0%
	GTAAATGCAGCCCAATTCGC+CGG	+	chr3_3:70075817-70075836	None:intergenic	50.0%
	TGATTGGGATTCCATCGTCG+TGG	+	chr3_3:70075854-70075873	None:intergenic	50.0%
	GCAAGCTTAGCGAATAGGCT+TGG	+	chr3_3:70076035-70076054	None:intergenic	50.0%
	GTAAATGCAGCCCAATTCGC+CGG	+	chr3_3:70075817-70075836	None:intergenic	50.0%
	TGATTGGGATTCCATCGTCG+TGG	+	chr3_3:70075854-70075873	None:intergenic	50.0%
	GCAAGCTTAGCGAATAGGCT+TGG	+	chr3_3:70076035-70076054	None:intergenic	50.0%
	ATCGTCGTGGAGGATCAAGG+TGG	+	chr3_3:70075841-70075860	None:intergenic	55.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr3_3:70075840-70075859	Msa0468440:three_prime_UTR	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr3_3:70075844-70075863	None:intergenic	55.0%
	TTGGGATTCCATCGTCGTGG+AGG	+	chr3_3:70075851-70075870	None:intergenic	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	chr3_3:70076080-70076099	Msa0468440:CDS	55.0%
!	CTTTTCAACGGCGGCAGGTT+CGG	+	chr3_3:70076162-70076181	None:intergenic	55.0%
	ACCTGCCGCCGTTGAAAAGA+AGG	-	chr3_3:70076163-70076182	Msa0468440:CDS	55.0%
!	ACCTTCTTTTCAACGGCGGC+AGG	+	chr3_3:70076167-70076186	None:intergenic	55.0%
	ATCGTCGTGGAGGATCAAGG+TGG	+	chr3_3:70075841-70075860	None:intergenic	55.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr3_3:70075840-70075859	Msa0468440:three_prime_UTR	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr3_3:70075844-70075863	None:intergenic	55.0%
	TTGGGATTCCATCGTCGTGG+AGG	+	chr3_3:70075851-70075870	None:intergenic	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	chr3_3:70076080-70076099	Msa0468440:CDS	55.0%
!	CTTTTCAACGGCGGCAGGTT+CGG	+	chr3_3:70076162-70076181	None:intergenic	55.0%
	ACCTGCCGCCGTTGAAAAGA+AGG	-	chr3_3:70076163-70076182	Msa0468440:CDS	55.0%
!	ACCTTCTTTTCAACGGCGGC+AGG	+	chr3_3:70076167-70076186	None:intergenic	55.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr3_3:70076083-70076102	Msa0468440:CDS	60.0%
	GGGTGCAGATACGGCAACAG+CGG	+	chr3_3:70076111-70076130	None:intergenic	60.0%
	ACCAGCAGCGGGTGCAGATA+CGG	+	chr3_3:70076120-70076139	None:intergenic	60.0%
	CGGATACAGAATCCCCTCCG+AGG	+	chr3_3:70076506-70076525	None:intergenic	60.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr3_3:70076083-70076102	Msa0468440:CDS	60.0%
	GGGTGCAGATACGGCAACAG+CGG	+	chr3_3:70076111-70076130	None:intergenic	60.0%
	ACCAGCAGCGGGTGCAGATA+CGG	+	chr3_3:70076120-70076139	None:intergenic	60.0%
	CGGATACAGAATCCCCTCCG+AGG	+	chr3_3:70076506-70076525	None:intergenic	60.0%
	GTATCTGCACCCGCTGCTGG+TGG	-	chr3_3:70076119-70076138	Msa0468440:CDS	65.0%
	GTATCTGCACCCGCTGCTGG+TGG	-	chr3_3:70076119-70076138	Msa0468440:CDS	65.0%
	AGCGGCACCACCAACAGCGC+CGG	+	chr3_3:70076093-70076112	None:intergenic	70.0%
	GCCGTATCTGCACCCGCTGC+TGG	-	chr3_3:70076116-70076135	Msa0468440:CDS	70.0%
	TCTGCACCCGCTGCTGGTGG+TGG	-	chr3_3:70076122-70076141	Msa0468440:CDS	70.0%
	TGCGGCTCCACCACCAGCAG+CGG	+	chr3_3:70076132-70076151	None:intergenic	70.0%
	GGCAGGTTCGGCAGCTGCTG+CGG	+	chr3_3:70076150-70076169	None:intergenic	70.0%
	AGCGGCACCACCAACAGCGC+CGG	+	chr3_3:70076093-70076112	None:intergenic	70.0%
	GCCGTATCTGCACCCGCTGC+TGG	-	chr3_3:70076116-70076135	Msa0468440:CDS	70.0%
	TCTGCACCCGCTGCTGGTGG+TGG	-	chr3_3:70076122-70076141	Msa0468440:CDS	70.0%
	TGCGGCTCCACCACCAGCAG+CGG	+	chr3_3:70076132-70076151	None:intergenic	70.0%
	GGCAGGTTCGGCAGCTGCTG+CGG	+	chr3_3:70076150-70076169	None:intergenic	70.0%
	GCGGCTCCACCACCAGCAGC+GGG	+	chr3_3:70076131-70076150	None:intergenic	75.0%
	GCGGCTCCACCACCAGCAGC+GGG	+	chr3_3:70076131-70076150	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	70075687	70076546	70075687	ID=Msa0468440;Name=Msa0468440
chr3_3	mRNA	70075687	70076546	70075687	ID=Msa0468440-mRNA-1;Parent=Msa0468440;Name=Msa0468440-mRNA-1;_AED=0.06;_eAED=0.06;_QI=120\|1\|1\|1\|1\|1\|2\|317\|107
chr3_3	exon	70075687	70076258	70075687	ID=Msa0468440-mRNA-1:exon:16801;Parent=Msa0468440-mRNA-1
chr3_3	exon	70076358	70076546	70076358	ID=Msa0468440-mRNA-1:exon:16800;Parent=Msa0468440-mRNA-1
chr3_3	five_prime_UTR	70076427	70076546	70076427	ID=Msa0468440-mRNA-1:five_prime_utr;Parent=Msa0468440-mRNA-1
chr3_3	CDS	70076358	70076426	70076358	ID=Msa0468440-mRNA-1:cds;Parent=Msa0468440-mRNA-1
chr3_3	CDS	70076004	70076258	70076004	ID=Msa0468440-mRNA-1:cds;Parent=Msa0468440-mRNA-1
chr3_3	three_prime_UTR	70075687	70076003	70075687	ID=Msa0468440-mRNA-1:three_prime_utr;Parent=Msa0468440-mRNA-1

Gene Sequence	>Msa0468440 ATGAGTTCCGGCGAATTGGGCTGCATTTACGCCACCTTGATCCTCCACGACGATGGAATCCCAATCACCGCGGAGAAGATCGGTACTTTGTTGAAGGCTGCTAACGTCACCGTTGAATCTTACTGGCCAAGCCTATTCGCTAAGCTTGCTCAGAGCAAGAACGTTGATGATCTCGTTTTGAACTCCGGCGCTGTTGGTGGTGCCGCTGTTGCCGTATCTGCACCCGCTGCTGGTGGTGGAGCCGCAGCAGCTGCCGAACCTGCCGCCGTTGAAAAGAAGGTATTGTTTTTAATTCTTTTTTTTATGATTATGATTTTTGTATGA
Protein sequence	>Msa0468440 MSSGELGCIYATLILHDDGIPITAEKIGTLLKAANVTVESYWPSLFAKLAQSKNVDDLVLNSGAVGGAAVAVSAPAAGGGAAAAAEPAAVEKKVLFLILFFMIMIFV*