AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1147670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1147670 MtrunA17_Chr7g0234631 98.496 133 2 0 1 133 1 133 1.17e-95 271
Msa1147670 MtrunA17_Chr8g0352341 96.992 133 4 0 1 133 1 133 1.21e-94 268
Msa1147670 MtrunA17_Chr7g0215831 96.992 133 4 0 1 133 1 133 6.01e-94 266
Msa1147670 MtrunA17_Chr8g0378631 67.669 133 9 1 1 133 1 99 1.21e-54 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1147670 AT4G18100.1 87.970 133 16 0 1 133 1 133 2.75e-87 250
Msa1147670 AT5G46430.2 85.714 133 19 0 1 133 1 133 2.47e-86 248
Msa1147670 AT5G46430.1 85.714 133 19 0 1 133 1 133 2.47e-86 248

Find 41 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTTGTTTAGCGTATTT+TGG 0.214895 7_4:+27042740 Msa1147670:three_prime_UTR
ACGCTTCTTCACAATCTTCT+TGG 0.281194 7_4:-27041564 None:intergenic
CGTTGAACGTGCGGCACAAT+TGG 0.294789 7_4:+27042529 Msa1147670:CDS
TTCTCAGGATTGTTGTCTAA+TGG 0.297002 7_4:+27042670 Msa1147670:three_prime_UTR
ACCCGCCACTATTTGCCTAA+TGG 0.370226 7_4:+27042305 Msa1147670:CDS
AGGGTATTGATTCACGAGTT+AGG 0.379946 7_4:+27042225 Msa1147670:CDS
CAAAGCTGGCGCAGGCCAAA+GGG 0.395273 7_4:+27042206 Msa1147670:CDS
GCGCAACCTGGCCAGCTTGT+TGG 0.406434 7_4:-27042564 None:intergenic
GCAAAGCTGGCGCAGGCCAA+AGG 0.427216 7_4:+27042205 Msa1147670:intron
ACAAACTTCTTGAATCCATT+AGG 0.438510 7_4:-27042320 None:intergenic
CATGGCAGACAATTGTTCTC+AGG 0.447217 7_4:+27042655 Msa1147670:three_prime_UTR
CTATGCAGCAAAGCTGGCGC+AGG 0.457182 7_4:+27042198 Msa1147670:intron
TTGTCACCAACAAGCTGGCC+AGG 0.462847 7_4:+27042558 Msa1147670:CDS
GTTGTGCTATGCAGCAAAGC+TGG 0.468798 7_4:+27042192 Msa1147670:intron
TCATCAGGCCACAGAGTGAC+AGG 0.478689 7_4:+27041597 Msa1147670:CDS
TGAATCCATTAGGCAAATAG+TGG 0.478886 7_4:-27042310 None:intergenic
GCTTGTTTCTGATTTGTGAC+TGG 0.484203 7_4:+27042849 Msa1147670:three_prime_UTR
TCAACAAAAGCTCCAGGTCC+TGG 0.484927 7_4:-27042355 None:intergenic
AGCGTGTCAAGAGGTTCATC+AGG 0.489670 7_4:+27041582 Msa1147670:CDS
TTGATGCCTAACATTGGATA+CGG 0.494285 7_4:+27042269 Msa1147670:CDS
ACAATCTTCTTGGTGAGCAA+TGG 0.494679 7_4:-27041554 None:intergenic
ACTCGTGAATCAATACCCTT+TGG 0.495359 7_4:-27042221 None:intergenic
GTTTGTCGTGCACAATGTCC+AGG 0.506762 7_4:+27042337 Msa1147670:CDS
TCTGAGCCGTATCCAATGTT+AGG 0.508029 7_4:-27042275 None:intergenic
CGTGCACAATGTCCAGGACC+TGG 0.538275 7_4:+27042343 Msa1147670:CDS
GTCTTCTTGACTGCGCAACC+TGG 0.545129 7_4:-27042576 None:intergenic
TCCATTAGGCAAATAGTGGC+GGG 0.556875 7_4:-27042306 None:intergenic
TCTGAAGCAGCGATTCAAGC+AGG 0.569830 7_4:+27040674 Msa1147670:five_prime_UTR
GTGACTTTGATGCCTAACAT+TGG 0.577268 7_4:+27042263 Msa1147670:CDS
TGTGCATCAACAAAAGCTCC+AGG 0.581714 7_4:-27042361 None:intergenic
ATCCATTAGGCAAATAGTGG+CGG 0.593712 7_4:-27042307 None:intergenic
TGTGTCTTGTAGAGACAAGA+TGG 0.594707 7_4:+27041526 Msa1147670:intron
CGAGTTAGGAGAAAATTCAA+AGG 0.594861 7_4:+27042239 Msa1147670:CDS
TGACAGGAAAATATGTGTGA+AGG 0.603768 7_4:+27041613 Msa1147670:CDS
AGAGATGTAAGAAGCAAATG+AGG 0.609147 7_4:-27040654 None:intergenic
AAAGGAGATCGTTGAACGTG+CGG 0.614630 7_4:+27042520 Msa1147670:CDS
TGTTGTTGTCACCAACAAGC+TGG 0.626162 7_4:+27042553 Msa1147670:CDS
TTGTGAAGAAGCGTGTCAAG+AGG 0.636300 7_4:+27041573 Msa1147670:CDS
CTTCTTGGTGAGCAATGGAA+CGG 0.637090 7_4:-27041549 None:intergenic
AGTCACAAATCAGAAACAAG+CGG 0.656417 7_4:-27042847 None:intergenic
TGTTGTTTATGGTATGAACA+TGG 0.659331 7_4:+27042637 Msa1147670:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGAAAGAATATTAATTA+GGG - chr7_4:27040711-27040730 None:intergenic 10.0%
!! ATAATGAAAGAATATTAATT+AGG - chr7_4:27040712-27040731 None:intergenic 10.0%
!! ACAAAATTAATAAACAAAAA+TGG - chr7_4:27040800-27040819 None:intergenic 10.0%
!! TAATGAAAGAATATTAATTA+GGG - chr7_4:27040711-27040730 None:intergenic 10.0%
!! ATAATGAAAGAATATTAATT+AGG - chr7_4:27040712-27040731 None:intergenic 10.0%
!! ACAAAATTAATAAACAAAAA+TGG - chr7_4:27040800-27040819 None:intergenic 10.0%
!!! ACCTAATTTGATTTTTTTTT+TGG + chr7_4:27040874-27040893 Msa1147670:intron 15.0%
!!! CCTAATTTGATTTTTTTTTT+GGG + chr7_4:27040875-27040894 Msa1147670:intron 15.0%
!!! GTATTTTCATAATTTCTTAA+CGG + chr7_4:27041335-27041354 Msa1147670:intron 15.0%
!! AATCAATTACATAAAACAAA+GGG - chr7_4:27041483-27041502 None:intergenic 15.0%
!! TAATCAATTACATAAAACAA+AGG - chr7_4:27041484-27041503 None:intergenic 15.0%
!! TAAATGAGTGTAATAATAAA+TGG - chr7_4:27041922-27041941 None:intergenic 15.0%
!! AGTAAAAAATAATAGATAAC+AGG - chr7_4:27042085-27042104 None:intergenic 15.0%
!! ATACATAACAACAAATAAAA+TGG - chr7_4:27042109-27042128 None:intergenic 15.0%
!!! ACCTAATTTGATTTTTTTTT+TGG + chr7_4:27040874-27040893 Msa1147670:intron 15.0%
!!! CCTAATTTGATTTTTTTTTT+GGG + chr7_4:27040875-27040894 Msa1147670:intron 15.0%
!!! GTATTTTCATAATTTCTTAA+CGG + chr7_4:27041335-27041354 Msa1147670:intron 15.0%
!! AATCAATTACATAAAACAAA+GGG - chr7_4:27041483-27041502 None:intergenic 15.0%
!! TAATCAATTACATAAAACAA+AGG - chr7_4:27041484-27041503 None:intergenic 15.0%
!! TAAATGAGTGTAATAATAAA+TGG - chr7_4:27041922-27041941 None:intergenic 15.0%
!! AGTAAAAAATAATAGATAAC+AGG - chr7_4:27042085-27042104 None:intergenic 15.0%
!! ATACATAACAACAAATAAAA+TGG - chr7_4:27042109-27042128 None:intergenic 15.0%
!! CCCAAAAAAAAAATCAAATT+AGG - chr7_4:27040878-27040897 None:intergenic 20.0%
!!! TTTGATTTTCTGAAATCTTA+TGG + chr7_4:27041077-27041096 Msa1147670:intron 20.0%
!! TAATTCAACAAAACATACAA+GGG - chr7_4:27041218-27041237 None:intergenic 20.0%
!! TTAATTCAACAAAACATACA+AGG - chr7_4:27041219-27041238 None:intergenic 20.0%
!!! TTGTATGTTTTGTTGAATTA+AGG + chr7_4:27041218-27041237 Msa1147670:intron 20.0%
!!! TGTATGTTTTGTTGAATTAA+GGG + chr7_4:27041219-27041238 Msa1147670:intron 20.0%
!! AATAGATAAGCTTTCAAAAA+TGG + chr7_4:27041644-27041663 Msa1147670:intron 20.0%
!!! TTTTTTTATCTGCAAACTAT+AGG + chr7_4:27041688-27041707 Msa1147670:intron 20.0%
!! AGATTAAATGTTATGATTTC+AGG + chr7_4:27041801-27041820 Msa1147670:intron 20.0%
!! GATTAAATGTTATGATTTCA+GGG + chr7_4:27041802-27041821 Msa1147670:intron 20.0%
!!! CATTTTCAGCAAAAATAATT+AGG - chr7_4:27041864-27041883 None:intergenic 20.0%
!!! CATTTTCAGCAAAAATAATT+AGG - chr7_4:27041864-27041883 None:intergenic 20.0%
!!! ATTTTTGCTTTTGTTGTTTA+TGG + chr7_4:27042626-27042645 Msa1147670:three_prime_UTR 20.0%
!!! ATAATTTGTTTAGCGTATTT+TGG + chr7_4:27042740-27042759 Msa1147670:three_prime_UTR 20.0%
!! CCCAAAAAAAAAATCAAATT+AGG - chr7_4:27040878-27040897 None:intergenic 20.0%
!!! TTTGATTTTCTGAAATCTTA+TGG + chr7_4:27041077-27041096 Msa1147670:intron 20.0%
!! TAATTCAACAAAACATACAA+GGG - chr7_4:27041218-27041237 None:intergenic 20.0%
!! TTAATTCAACAAAACATACA+AGG - chr7_4:27041219-27041238 None:intergenic 20.0%
!!! TTGTATGTTTTGTTGAATTA+AGG + chr7_4:27041218-27041237 Msa1147670:intron 20.0%
!!! TGTATGTTTTGTTGAATTAA+GGG + chr7_4:27041219-27041238 Msa1147670:intron 20.0%
!! AATAGATAAGCTTTCAAAAA+TGG + chr7_4:27041644-27041663 Msa1147670:intron 20.0%
!!! TTTTTTTATCTGCAAACTAT+AGG + chr7_4:27041688-27041707 Msa1147670:intron 20.0%
!! AGATTAAATGTTATGATTTC+AGG + chr7_4:27041801-27041820 Msa1147670:intron 20.0%
!! GATTAAATGTTATGATTTCA+GGG + chr7_4:27041802-27041821 Msa1147670:intron 20.0%
!!! CATTTTCAGCAAAAATAATT+AGG - chr7_4:27041864-27041883 None:intergenic 20.0%
!!! CATTTTCAGCAAAAATAATT+AGG - chr7_4:27041864-27041883 None:intergenic 20.0%
!!! ATTTTTGCTTTTGTTGTTTA+TGG + chr7_4:27042626-27042645 Msa1147670:three_prime_UTR 20.0%
!!! ATAATTTGTTTAGCGTATTT+TGG + chr7_4:27042740-27042759 Msa1147670:three_prime_UTR 20.0%
!!! TTTTGAGTGAAATACAAACA+TGG + chr7_4:27040909-27040928 Msa1147670:intron 25.0%
! TTTGAGTGAAATACAAACAT+GGG + chr7_4:27040910-27040929 Msa1147670:intron 25.0%
!! TTTTCATAAGCTCAAAAACA+AGG - chr7_4:27041258-27041277 None:intergenic 25.0%
!! TCTAAACAGAAAACACTTTT+AGG - chr7_4:27041402-27041421 None:intergenic 25.0%
!!! ATTTGTTGTTATGTATATGC+TGG + chr7_4:27042113-27042132 Msa1147670:intron 25.0%
! TATTCAACACAAAAAACTCT+GGG - chr7_4:27042712-27042731 None:intergenic 25.0%
!!! TTTTGAGTGAAATACAAACA+TGG + chr7_4:27040909-27040928 Msa1147670:intron 25.0%
! TTTGAGTGAAATACAAACAT+GGG + chr7_4:27040910-27040929 Msa1147670:intron 25.0%
!! TTTTCATAAGCTCAAAAACA+AGG - chr7_4:27041258-27041277 None:intergenic 25.0%
!! TCTAAACAGAAAACACTTTT+AGG - chr7_4:27041402-27041421 None:intergenic 25.0%
!!! ATTTGTTGTTATGTATATGC+TGG + chr7_4:27042113-27042132 Msa1147670:intron 25.0%
! TATTCAACACAAAAAACTCT+GGG - chr7_4:27042712-27042731 None:intergenic 25.0%
!! TGAAGCTTTGAACTGAATTA+TGG + chr7_4:27040851-27040870 Msa1147670:intron 30.0%
!!! AAATGAAGCTTGTTTTGTGA+TGG + chr7_4:27040949-27040968 Msa1147670:intron 30.0%
!!! AATGAAGCTTGTTTTGTGAT+GGG + chr7_4:27040950-27040969 Msa1147670:intron 30.0%
!! TGGGTTTTGTAATCGAAATT+AGG + chr7_4:27040969-27040988 Msa1147670:intron 30.0%
!! GGGTTTTGTAATCGAAATTA+GGG + chr7_4:27040970-27040989 Msa1147670:intron 30.0%
!! TTTCTGAAATCTTATGGTGT+AGG + chr7_4:27041083-27041102 Msa1147670:intron 30.0%
AAATCCTACACCACATTATT+GGG - chr7_4:27041144-27041163 None:intergenic 30.0%
TAAATCCTACACCACATTAT+TGG - chr7_4:27041145-27041164 None:intergenic 30.0%
! TAGTTTTTATCATGCTCCTA+AGG + chr7_4:27041184-27041203 Msa1147670:intron 30.0%
ACAAACTTCTTGAATCCATT+AGG - chr7_4:27042323-27042342 None:intergenic 30.0%
! TGTTGTTTATGGTATGAACA+TGG + chr7_4:27042637-27042656 Msa1147670:three_prime_UTR 30.0%
GTATTCAACACAAAAAACTC+TGG - chr7_4:27042713-27042732 None:intergenic 30.0%
!! TGAAGCTTTGAACTGAATTA+TGG + chr7_4:27040851-27040870 Msa1147670:intron 30.0%
!!! AAATGAAGCTTGTTTTGTGA+TGG + chr7_4:27040949-27040968 Msa1147670:intron 30.0%
!!! AATGAAGCTTGTTTTGTGAT+GGG + chr7_4:27040950-27040969 Msa1147670:intron 30.0%
!! TGGGTTTTGTAATCGAAATT+AGG + chr7_4:27040969-27040988 Msa1147670:intron 30.0%
!! GGGTTTTGTAATCGAAATTA+GGG + chr7_4:27040970-27040989 Msa1147670:intron 30.0%
!! TTTCTGAAATCTTATGGTGT+AGG + chr7_4:27041083-27041102 Msa1147670:intron 30.0%
AAATCCTACACCACATTATT+GGG - chr7_4:27041144-27041163 None:intergenic 30.0%
TAAATCCTACACCACATTAT+TGG - chr7_4:27041145-27041164 None:intergenic 30.0%
! TAGTTTTTATCATGCTCCTA+AGG + chr7_4:27041184-27041203 Msa1147670:intron 30.0%
ACAAACTTCTTGAATCCATT+AGG - chr7_4:27042323-27042342 None:intergenic 30.0%
! TGTTGTTTATGGTATGAACA+TGG + chr7_4:27042637-27042656 Msa1147670:three_prime_UTR 30.0%
GTATTCAACACAAAAAACTC+TGG - chr7_4:27042713-27042732 None:intergenic 30.0%
AGAGATGTAAGAAGCAAATG+AGG - chr7_4:27040657-27040676 None:intergenic 35.0%
TTGTTCTTATTCTCTCGATC+TGG + chr7_4:27040816-27040835 Msa1147670:intron 35.0%
! TCTCGATCTGGTTTCATAAA+AGG + chr7_4:27040828-27040847 Msa1147670:intron 35.0%
!!! ACATGGGTCGAATTGTTTTT+TGG + chr7_4:27040926-27040945 Msa1147670:intron 35.0%
TTATCATGCTCCCAATAATG+TGG + chr7_4:27041131-27041150 Msa1147670:intron 35.0%
!! GTTGAATTAAGGGCTGTTTT+AGG + chr7_4:27041229-27041248 Msa1147670:intron 35.0%
TGACAGGAAAATATGTGTGA+AGG + chr7_4:27041613-27041632 Msa1147670:CDS 35.0%
AGGTACATGCGTGTAATAAA+TGG - chr7_4:27041844-27041863 None:intergenic 35.0%
TGCATAGCACAACAGTAATA+AGG - chr7_4:27042185-27042204 None:intergenic 35.0%
CGAGTTAGGAGAAAATTCAA+AGG + chr7_4:27042239-27042258 Msa1147670:CDS 35.0%
TTGATGCCTAACATTGGATA+CGG + chr7_4:27042269-27042288 Msa1147670:CDS 35.0%
TGAATCCATTAGGCAAATAG+TGG - chr7_4:27042313-27042332 None:intergenic 35.0%
TGTGTCAACCAGAAAAAGAA+AGG + chr7_4:27042502-27042521 Msa1147670:CDS 35.0%
! AACGATCTCCTTTCTTTTTC+TGG - chr7_4:27042513-27042532 None:intergenic 35.0%
TTCTCAGGATTGTTGTCTAA+TGG + chr7_4:27042670-27042689 Msa1147670:three_prime_UTR 35.0%
AGTCACAAATCAGAAACAAG+CGG - chr7_4:27042850-27042869 None:intergenic 35.0%
AGAGATGTAAGAAGCAAATG+AGG - chr7_4:27040657-27040676 None:intergenic 35.0%
TTGTTCTTATTCTCTCGATC+TGG + chr7_4:27040816-27040835 Msa1147670:intron 35.0%
! TCTCGATCTGGTTTCATAAA+AGG + chr7_4:27040828-27040847 Msa1147670:intron 35.0%
!!! ACATGGGTCGAATTGTTTTT+TGG + chr7_4:27040926-27040945 Msa1147670:intron 35.0%
TTATCATGCTCCCAATAATG+TGG + chr7_4:27041131-27041150 Msa1147670:intron 35.0%
!! GTTGAATTAAGGGCTGTTTT+AGG + chr7_4:27041229-27041248 Msa1147670:intron 35.0%
TGACAGGAAAATATGTGTGA+AGG + chr7_4:27041613-27041632 Msa1147670:CDS 35.0%
AGGTACATGCGTGTAATAAA+TGG - chr7_4:27041844-27041863 None:intergenic 35.0%
TGCATAGCACAACAGTAATA+AGG - chr7_4:27042185-27042204 None:intergenic 35.0%
CGAGTTAGGAGAAAATTCAA+AGG + chr7_4:27042239-27042258 Msa1147670:CDS 35.0%
TTGATGCCTAACATTGGATA+CGG + chr7_4:27042269-27042288 Msa1147670:CDS 35.0%
TGAATCCATTAGGCAAATAG+TGG - chr7_4:27042313-27042332 None:intergenic 35.0%
TGTGTCAACCAGAAAAAGAA+AGG + chr7_4:27042502-27042521 Msa1147670:CDS 35.0%
! AACGATCTCCTTTCTTTTTC+TGG - chr7_4:27042513-27042532 None:intergenic 35.0%
TTCTCAGGATTGTTGTCTAA+TGG + chr7_4:27042670-27042689 Msa1147670:three_prime_UTR 35.0%
AGTCACAAATCAGAAACAAG+CGG - chr7_4:27042850-27042869 None:intergenic 35.0%
TCAGAAGCAGCATCGTATAA+AGG - chr7_4:27040771-27040790 None:intergenic 40.0%
AAGCTAAACCAGAACACACA+AGG - chr7_4:27041001-27041020 None:intergenic 40.0%
TATCATGCTCCTAAGGAGTA+AGG + chr7_4:27041191-27041210 Msa1147670:intron 40.0%
TGTGTCTTGTAGAGACAAGA+TGG + chr7_4:27041526-27041545 Msa1147670:intron 40.0%
ACAATCTTCTTGGTGAGCAA+TGG - chr7_4:27041557-27041576 None:intergenic 40.0%
ACGCTTCTTCACAATCTTCT+TGG - chr7_4:27041567-27041586 None:intergenic 40.0%
! CATATTTTCCTGTCACTCTG+TGG - chr7_4:27041608-27041627 None:intergenic 40.0%
AAAGCTCAAACATACCACGA+AGG - chr7_4:27041714-27041733 None:intergenic 40.0%
TAACAGGTATCAGTAGCAGT+AGG - chr7_4:27042069-27042088 None:intergenic 40.0%
ACTCGTGAATCAATACCCTT+TGG - chr7_4:27042224-27042243 None:intergenic 40.0%
!! AGGGTATTGATTCACGAGTT+AGG + chr7_4:27042225-27042244 Msa1147670:CDS 40.0%
GTGACTTTGATGCCTAACAT+TGG + chr7_4:27042263-27042282 Msa1147670:CDS 40.0%
ATCCATTAGGCAAATAGTGG+CGG - chr7_4:27042310-27042329 None:intergenic 40.0%
! AGCTTTTGTTGATGCACAAC+AGG + chr7_4:27042366-27042385 Msa1147670:CDS 40.0%
AGTTACGAAGAGACAATGAG+AGG - chr7_4:27042424-27042443 None:intergenic 40.0%
CATTTACATGAACAACCCAC+AGG + chr7_4:27042453-27042472 Msa1147670:intron 40.0%
!! GCTTGTTTCTGATTTGTGAC+TGG + chr7_4:27042849-27042868 Msa1147670:three_prime_UTR 40.0%
TCAGAAGCAGCATCGTATAA+AGG - chr7_4:27040771-27040790 None:intergenic 40.0%
AAGCTAAACCAGAACACACA+AGG - chr7_4:27041001-27041020 None:intergenic 40.0%
TATCATGCTCCTAAGGAGTA+AGG + chr7_4:27041191-27041210 Msa1147670:intron 40.0%
TGTGTCTTGTAGAGACAAGA+TGG + chr7_4:27041526-27041545 Msa1147670:intron 40.0%
ACAATCTTCTTGGTGAGCAA+TGG - chr7_4:27041557-27041576 None:intergenic 40.0%
ACGCTTCTTCACAATCTTCT+TGG - chr7_4:27041567-27041586 None:intergenic 40.0%
! CATATTTTCCTGTCACTCTG+TGG - chr7_4:27041608-27041627 None:intergenic 40.0%
AAAGCTCAAACATACCACGA+AGG - chr7_4:27041714-27041733 None:intergenic 40.0%
TAACAGGTATCAGTAGCAGT+AGG - chr7_4:27042069-27042088 None:intergenic 40.0%
ACTCGTGAATCAATACCCTT+TGG - chr7_4:27042224-27042243 None:intergenic 40.0%
!! AGGGTATTGATTCACGAGTT+AGG + chr7_4:27042225-27042244 Msa1147670:CDS 40.0%
GTGACTTTGATGCCTAACAT+TGG + chr7_4:27042263-27042282 Msa1147670:CDS 40.0%
ATCCATTAGGCAAATAGTGG+CGG - chr7_4:27042310-27042329 None:intergenic 40.0%
! AGCTTTTGTTGATGCACAAC+AGG + chr7_4:27042366-27042385 Msa1147670:CDS 40.0%
AGTTACGAAGAGACAATGAG+AGG - chr7_4:27042424-27042443 None:intergenic 40.0%
CATTTACATGAACAACCCAC+AGG + chr7_4:27042453-27042472 Msa1147670:intron 40.0%
!! GCTTGTTTCTGATTTGTGAC+TGG + chr7_4:27042849-27042868 Msa1147670:three_prime_UTR 40.0%
TGCTCCCAATAATGTGGTGT+AGG + chr7_4:27041137-27041156 Msa1147670:intron 45.0%
TACAAGGGTCCTTACTCCTT+AGG - chr7_4:27041203-27041222 None:intergenic 45.0%
CTTCTTGGTGAGCAATGGAA+CGG - chr7_4:27041552-27041571 None:intergenic 45.0%
! TTGTGAAGAAGCGTGTCAAG+AGG + chr7_4:27041573-27041592 Msa1147670:CDS 45.0%
AGGTATCAGTAGCAGTAGGT+AGG - chr7_4:27042065-27042084 None:intergenic 45.0%
TCTGAGCCGTATCCAATGTT+AGG - chr7_4:27042278-27042297 None:intergenic 45.0%
TCCATTAGGCAAATAGTGGC+GGG - chr7_4:27042309-27042328 None:intergenic 45.0%
TGTGCATCAACAAAAGCTCC+AGG - chr7_4:27042364-27042383 None:intergenic 45.0%
GTTGATGCACAACAGGTAAC+TGG + chr7_4:27042373-27042392 Msa1147670:intron 45.0%
AAAGGAGATCGTTGAACGTG+CGG + chr7_4:27042520-27042539 Msa1147670:CDS 45.0%
TGTTGTTGTCACCAACAAGC+TGG + chr7_4:27042553-27042572 Msa1147670:CDS 45.0%
CATGGCAGACAATTGTTCTC+AGG + chr7_4:27042655-27042674 Msa1147670:three_prime_UTR 45.0%
TGCTCCCAATAATGTGGTGT+AGG + chr7_4:27041137-27041156 Msa1147670:intron 45.0%
TACAAGGGTCCTTACTCCTT+AGG - chr7_4:27041203-27041222 None:intergenic 45.0%
CTTCTTGGTGAGCAATGGAA+CGG - chr7_4:27041552-27041571 None:intergenic 45.0%
! TTGTGAAGAAGCGTGTCAAG+AGG + chr7_4:27041573-27041592 Msa1147670:CDS 45.0%
AGGTATCAGTAGCAGTAGGT+AGG - chr7_4:27042065-27042084 None:intergenic 45.0%
TCTGAGCCGTATCCAATGTT+AGG - chr7_4:27042278-27042297 None:intergenic 45.0%
TCCATTAGGCAAATAGTGGC+GGG - chr7_4:27042309-27042328 None:intergenic 45.0%
TGTGCATCAACAAAAGCTCC+AGG - chr7_4:27042364-27042383 None:intergenic 45.0%
GTTGATGCACAACAGGTAAC+TGG + chr7_4:27042373-27042392 Msa1147670:intron 45.0%
AAAGGAGATCGTTGAACGTG+CGG + chr7_4:27042520-27042539 Msa1147670:CDS 45.0%
TGTTGTTGTCACCAACAAGC+TGG + chr7_4:27042553-27042572 Msa1147670:CDS 45.0%
CATGGCAGACAATTGTTCTC+AGG + chr7_4:27042655-27042674 Msa1147670:three_prime_UTR 45.0%
! TCTGAAGCAGCGATTCAAGC+AGG + chr7_4:27040674-27040693 Msa1147670:five_prime_UTR 50.0%
AGCGTGTCAAGAGGTTCATC+AGG + chr7_4:27041582-27041601 Msa1147670:CDS 50.0%
CTGCAAACTATAGGCCTTCG+TGG + chr7_4:27041697-27041716 Msa1147670:intron 50.0%
GTTGTGCTATGCAGCAAAGC+TGG + chr7_4:27042192-27042211 Msa1147670:intron 50.0%
ACCCGCCACTATTTGCCTAA+TGG + chr7_4:27042305-27042324 Msa1147670:CDS 50.0%
GTTTGTCGTGCACAATGTCC+AGG + chr7_4:27042337-27042356 Msa1147670:CDS 50.0%
TCAACAAAAGCTCCAGGTCC+TGG - chr7_4:27042358-27042377 None:intergenic 50.0%
CTCAGCACAGTATGTCCTGT+GGG - chr7_4:27042471-27042490 None:intergenic 50.0%
TCTCAGCACAGTATGTCCTG+TGG - chr7_4:27042472-27042491 None:intergenic 50.0%
! TCTGAAGCAGCGATTCAAGC+AGG + chr7_4:27040674-27040693 Msa1147670:five_prime_UTR 50.0%
AGCGTGTCAAGAGGTTCATC+AGG + chr7_4:27041582-27041601 Msa1147670:CDS 50.0%
CTGCAAACTATAGGCCTTCG+TGG + chr7_4:27041697-27041716 Msa1147670:intron 50.0%
GTTGTGCTATGCAGCAAAGC+TGG + chr7_4:27042192-27042211 Msa1147670:intron 50.0%
ACCCGCCACTATTTGCCTAA+TGG + chr7_4:27042305-27042324 Msa1147670:CDS 50.0%
GTTTGTCGTGCACAATGTCC+AGG + chr7_4:27042337-27042356 Msa1147670:CDS 50.0%
TCAACAAAAGCTCCAGGTCC+TGG - chr7_4:27042358-27042377 None:intergenic 50.0%
CTCAGCACAGTATGTCCTGT+GGG - chr7_4:27042471-27042490 None:intergenic 50.0%
TCTCAGCACAGTATGTCCTG+TGG - chr7_4:27042472-27042491 None:intergenic 50.0%
! GGGTTGAGCCTTGTGTGTTC+TGG + chr7_4:27040990-27041009 Msa1147670:intron 55.0%
TCATCAGGCCACAGAGTGAC+AGG + chr7_4:27041597-27041616 Msa1147670:CDS 55.0%
CGTTGAACGTGCGGCACAAT+TGG + chr7_4:27042529-27042548 Msa1147670:CDS 55.0%
TTGTCACCAACAAGCTGGCC+AGG + chr7_4:27042558-27042577 Msa1147670:CDS 55.0%
GTCTTCTTGACTGCGCAACC+TGG - chr7_4:27042579-27042598 None:intergenic 55.0%
! GGGTTGAGCCTTGTGTGTTC+TGG + chr7_4:27040990-27041009 Msa1147670:intron 55.0%
TCATCAGGCCACAGAGTGAC+AGG + chr7_4:27041597-27041616 Msa1147670:CDS 55.0%
CGTTGAACGTGCGGCACAAT+TGG + chr7_4:27042529-27042548 Msa1147670:CDS 55.0%
TTGTCACCAACAAGCTGGCC+AGG + chr7_4:27042558-27042577 Msa1147670:CDS 55.0%
GTCTTCTTGACTGCGCAACC+TGG - chr7_4:27042579-27042598 None:intergenic 55.0%
CTATGCAGCAAAGCTGGCGC+AGG + chr7_4:27042198-27042217 Msa1147670:intron 60.0%
CAAAGCTGGCGCAGGCCAAA+GGG + chr7_4:27042206-27042225 Msa1147670:CDS 60.0%
CGTGCACAATGTCCAGGACC+TGG + chr7_4:27042343-27042362 Msa1147670:CDS 60.0%
CTATGCAGCAAAGCTGGCGC+AGG + chr7_4:27042198-27042217 Msa1147670:intron 60.0%
CAAAGCTGGCGCAGGCCAAA+GGG + chr7_4:27042206-27042225 Msa1147670:CDS 60.0%
CGTGCACAATGTCCAGGACC+TGG + chr7_4:27042343-27042362 Msa1147670:CDS 60.0%
GCAAAGCTGGCGCAGGCCAA+AGG + chr7_4:27042205-27042224 Msa1147670:intron 65.0%
GCGCAACCTGGCCAGCTTGT+TGG - chr7_4:27042567-27042586 None:intergenic 65.0%
GCAAAGCTGGCGCAGGCCAA+AGG + chr7_4:27042205-27042224 Msa1147670:intron 65.0%
GCGCAACCTGGCCAGCTTGT+TGG - chr7_4:27042567-27042586 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 27040649 27042901 27040649 ID=Msa1147670;Name=Msa1147670
chr7_4 mRNA 27040649 27042901 27040649 ID=Msa1147670-mRNA-1;Parent=Msa1147670;Name=Msa1147670-mRNA-1;_AED=0.06;_eAED=0.06;_QI=54|1|1|1|0.66|0.75|4|297|133
chr7_4 exon 27040649 27040695 27040649 ID=Msa1147670-mRNA-1:exon:6615;Parent=Msa1147670-mRNA-1
chr7_4 exon 27041538 27041634 27041538 ID=Msa1147670-mRNA-1:exon:6616;Parent=Msa1147670-mRNA-1
chr7_4 exon 27042206 27042387 27042206 ID=Msa1147670-mRNA-1:exon:6617;Parent=Msa1147670-mRNA-1
chr7_4 exon 27042475 27042901 27042475 ID=Msa1147670-mRNA-1:exon:6618;Parent=Msa1147670-mRNA-1
chr7_4 five_prime_UTR 27040649 27040695 27040649 ID=Msa1147670-mRNA-1:five_prime_utr;Parent=Msa1147670-mRNA-1
chr7_4 five_prime_UTR 27041538 27041544 27041538 ID=Msa1147670-mRNA-1:five_prime_utr;Parent=Msa1147670-mRNA-1
chr7_4 CDS 27041545 27041634 27041545 ID=Msa1147670-mRNA-1:cds;Parent=Msa1147670-mRNA-1
chr7_4 CDS 27042206 27042387 27042206 ID=Msa1147670-mRNA-1:cds;Parent=Msa1147670-mRNA-1
chr7_4 CDS 27042475 27042604 27042475 ID=Msa1147670-mRNA-1:cds;Parent=Msa1147670-mRNA-1
chr7_4 three_prime_UTR 27042605 27042901 27042605 ID=Msa1147670-mRNA-1:three_prime_utr;Parent=Msa1147670-mRNA-1
Gene Sequence

>Msa1147670

ATGGCCGTTCCATTGCTCACCAAGAAGATTGTGAAGAAGCGTGTCAAGAGGTTCATCAGGCCACAGAGTGACAGGAAAATATGTGTGAAGCAAAGCTGGCGCAGGCCAAAGGGTATTGATTCACGAGTTAGGAGAAAATTCAAAGGAGTGACTTTGATGCCTAACATTGGATACGGCTCAGACAAGAAGACCCGCCACTATTTGCCTAATGGATTCAAGAAGTTTGTCGTGCACAATGTCCAGGACCTGGAGCTTTTGTTGATGCACAACAGGACATACTGTGCTGAGATTGCACACAATGTGTCAACCAGAAAAAGAAAGGAGATCGTTGAACGTGCGGCACAATTGGATGTTGTTGTCACCAACAAGCTGGCCAGGTTGCGCAGTCAAGAAGACGAGTGA
Protein sequence

>Msa1147670

MAVPLLTKKIVKKRVKRFIRPQSDRKICVKQSWRRPKGIDSRVRRKFKGVTLMPNIGYGSDKKTRHYLPNGFKKFVVHNVQDLELLLMHNRTYCAEIAHNVSTRKRKEIVERAAQLDVVVTNKLARLRSQEDE*