AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1397660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1397660 MtrunA17_Chr7g0234631 98.496 133 2 0 1 133 1 133 1.17e-95 271
Msa1397660 MtrunA17_Chr8g0352341 96.992 133 4 0 1 133 1 133 1.21e-94 268
Msa1397660 MtrunA17_Chr7g0215831 96.992 133 4 0 1 133 1 133 6.01e-94 266
Msa1397660 MtrunA17_Chr8g0378631 67.669 133 9 1 1 133 1 99 1.21e-54 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1397660 AT4G18100.1 87.970 133 16 0 1 133 1 133 2.75e-87 250
Msa1397660 AT5G46430.2 85.714 133 19 0 1 133 1 133 2.47e-86 248
Msa1397660 AT5G46430.1 85.714 133 19 0 1 133 1 133 2.47e-86 248

Find 41 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTTGTTTAGCGTATTT+TGG 0.214895 tig0022176:+17691 Msa1397660:three_prime_UTR
ACGCTTCTTCACAATCTTCT+TGG 0.281194 tig0022176:-16515 None:intergenic
CGTTGAACGTGCGGCACAAT+TGG 0.294789 tig0022176:+17480 Msa1397660:CDS
TTCTCAGGATTGTTGTCTAA+TGG 0.297002 tig0022176:+17621 Msa1397660:three_prime_UTR
ACCCGCCACTATTTGCCTAA+TGG 0.370226 tig0022176:+17256 Msa1397660:CDS
AGGGTATTGATTCACGAGTT+AGG 0.379946 tig0022176:+17176 Msa1397660:CDS
CAAAGCTGGCGCAGGCCAAA+GGG 0.395273 tig0022176:+17157 Msa1397660:CDS
GCGCAACCTGGCCAGCTTGT+TGG 0.406434 tig0022176:-17515 None:intergenic
GCAAAGCTGGCGCAGGCCAA+AGG 0.427216 tig0022176:+17156 Msa1397660:intron
ACAAACTTCTTGAATCCATT+AGG 0.438510 tig0022176:-17271 None:intergenic
CATGGCAGACAATTGTTCTC+AGG 0.447217 tig0022176:+17606 Msa1397660:three_prime_UTR
CTATGCAGCAAAGCTGGCGC+AGG 0.457182 tig0022176:+17149 Msa1397660:intron
TTGTCACCAACAAGCTGGCC+AGG 0.462847 tig0022176:+17509 Msa1397660:CDS
GTTGTGCTATGCAGCAAAGC+TGG 0.468798 tig0022176:+17143 Msa1397660:intron
TCATCAGGCCACAGAGTGAC+AGG 0.478689 tig0022176:+16548 Msa1397660:CDS
TGAATCCATTAGGCAAATAG+TGG 0.478886 tig0022176:-17261 None:intergenic
GCTTGTTTCTGATTTGTGAC+TGG 0.484203 tig0022176:+17800 Msa1397660:three_prime_UTR
TCAACAAAAGCTCCAGGTCC+TGG 0.484927 tig0022176:-17306 None:intergenic
AGCGTGTCAAGAGGTTCATC+AGG 0.489670 tig0022176:+16533 Msa1397660:CDS
TTGATGCCTAACATTGGATA+CGG 0.494285 tig0022176:+17220 Msa1397660:CDS
ACAATCTTCTTGGTGAGCAA+TGG 0.494679 tig0022176:-16505 None:intergenic
ACTCGTGAATCAATACCCTT+TGG 0.495359 tig0022176:-17172 None:intergenic
GTTTGTCGTGCACAATGTCC+AGG 0.506762 tig0022176:+17288 Msa1397660:CDS
TCTGAGCCGTATCCAATGTT+AGG 0.508029 tig0022176:-17226 None:intergenic
CGTGCACAATGTCCAGGACC+TGG 0.538275 tig0022176:+17294 Msa1397660:CDS
GTCTTCTTGACTGCGCAACC+TGG 0.545129 tig0022176:-17527 None:intergenic
TCCATTAGGCAAATAGTGGC+GGG 0.556875 tig0022176:-17257 None:intergenic
TCTGAAGCAGCGATTCAAGC+AGG 0.569830 tig0022176:+15625 Msa1397660:five_prime_UTR
GTGACTTTGATGCCTAACAT+TGG 0.577268 tig0022176:+17214 Msa1397660:CDS
TGTGCATCAACAAAAGCTCC+AGG 0.581714 tig0022176:-17312 None:intergenic
ATCCATTAGGCAAATAGTGG+CGG 0.593712 tig0022176:-17258 None:intergenic
TGTGTCTTGTAGAGACAAGA+TGG 0.594707 tig0022176:+16477 Msa1397660:intron
CGAGTTAGGAGAAAATTCAA+AGG 0.594861 tig0022176:+17190 Msa1397660:CDS
TGACAGGAAAATATGTGTGA+AGG 0.603768 tig0022176:+16564 Msa1397660:CDS
AGAGATGTAAGAAGCAAATG+AGG 0.609147 tig0022176:-15605 None:intergenic
AAAGGAGATCGTTGAACGTG+CGG 0.614630 tig0022176:+17471 Msa1397660:CDS
TGTTGTTGTCACCAACAAGC+TGG 0.626162 tig0022176:+17504 Msa1397660:CDS
TTGTGAAGAAGCGTGTCAAG+AGG 0.636300 tig0022176:+16524 Msa1397660:CDS
CTTCTTGGTGAGCAATGGAA+CGG 0.637090 tig0022176:-16500 None:intergenic
AGTCACAAATCAGAAACAAG+CGG 0.656417 tig0022176:-17798 None:intergenic
TGTTGTTTATGGTATGAACA+TGG 0.659331 tig0022176:+17588 Msa1397660:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGAAAGAATATTAATTA+GGG - tig0022176:15662-15681 None:intergenic 10.0%
!! ATAATGAAAGAATATTAATT+AGG - tig0022176:15663-15682 None:intergenic 10.0%
!! ACAAAATTAATAAACAAAAA+TGG - tig0022176:15751-15770 None:intergenic 10.0%
!! TAATGAAAGAATATTAATTA+GGG - tig0022176:15662-15681 None:intergenic 10.0%
!! ATAATGAAAGAATATTAATT+AGG - tig0022176:15663-15682 None:intergenic 10.0%
!! ACAAAATTAATAAACAAAAA+TGG - tig0022176:15751-15770 None:intergenic 10.0%
!!! ACCTAATTTGATTTTTTTTT+TGG + tig0022176:15825-15844 Msa1397660:intron 15.0%
!!! CCTAATTTGATTTTTTTTTT+GGG + tig0022176:15826-15845 Msa1397660:intron 15.0%
!!! GTATTTTCATAATTTCTTAA+CGG + tig0022176:16286-16305 Msa1397660:intron 15.0%
!! AATCAATTACATAAAACAAA+GGG - tig0022176:16434-16453 None:intergenic 15.0%
!! TAATCAATTACATAAAACAA+AGG - tig0022176:16435-16454 None:intergenic 15.0%
!! TAAATGAGTGTAATAATAAA+TGG - tig0022176:16873-16892 None:intergenic 15.0%
!! AGTAAAAAATAATAGATAAC+AGG - tig0022176:17036-17055 None:intergenic 15.0%
!! ATACATAACAACAAATAAAA+TGG - tig0022176:17060-17079 None:intergenic 15.0%
!!! ACCTAATTTGATTTTTTTTT+TGG + tig0022176:15825-15844 Msa1397660:intron 15.0%
!!! CCTAATTTGATTTTTTTTTT+GGG + tig0022176:15826-15845 Msa1397660:intron 15.0%
!!! GTATTTTCATAATTTCTTAA+CGG + tig0022176:16286-16305 Msa1397660:intron 15.0%
!! AATCAATTACATAAAACAAA+GGG - tig0022176:16434-16453 None:intergenic 15.0%
!! TAATCAATTACATAAAACAA+AGG - tig0022176:16435-16454 None:intergenic 15.0%
!! TAAATGAGTGTAATAATAAA+TGG - tig0022176:16873-16892 None:intergenic 15.0%
!! AGTAAAAAATAATAGATAAC+AGG - tig0022176:17036-17055 None:intergenic 15.0%
!! ATACATAACAACAAATAAAA+TGG - tig0022176:17060-17079 None:intergenic 15.0%
!! CCCAAAAAAAAAATCAAATT+AGG - tig0022176:15829-15848 None:intergenic 20.0%
!!! TTTGATTTTCTGAAATCTTA+TGG + tig0022176:16028-16047 Msa1397660:intron 20.0%
!! TAATTCAACAAAACATACAA+GGG - tig0022176:16169-16188 None:intergenic 20.0%
!! TTAATTCAACAAAACATACA+AGG - tig0022176:16170-16189 None:intergenic 20.0%
!!! TTGTATGTTTTGTTGAATTA+AGG + tig0022176:16169-16188 Msa1397660:intron 20.0%
!!! TGTATGTTTTGTTGAATTAA+GGG + tig0022176:16170-16189 Msa1397660:intron 20.0%
!! AATAGATAAGCTTTCAAAAA+TGG + tig0022176:16595-16614 Msa1397660:intron 20.0%
!!! TTTTTTTATCTGCAAACTAT+AGG + tig0022176:16639-16658 Msa1397660:intron 20.0%
!! AGATTAAATGTTATGATTTC+AGG + tig0022176:16752-16771 Msa1397660:intron 20.0%
!! GATTAAATGTTATGATTTCA+GGG + tig0022176:16753-16772 Msa1397660:intron 20.0%
!!! CATTTTCAGCAAAAATAATT+AGG - tig0022176:16815-16834 None:intergenic 20.0%
!!! CATTTTCAGCAAAAATAATT+AGG - tig0022176:16815-16834 None:intergenic 20.0%
!!! ATTTTTGCTTTTGTTGTTTA+TGG + tig0022176:17577-17596 Msa1397660:three_prime_UTR 20.0%
!!! ATAATTTGTTTAGCGTATTT+TGG + tig0022176:17691-17710 Msa1397660:three_prime_UTR 20.0%
!! CCCAAAAAAAAAATCAAATT+AGG - tig0022176:15829-15848 None:intergenic 20.0%
!!! TTTGATTTTCTGAAATCTTA+TGG + tig0022176:16028-16047 Msa1397660:intron 20.0%
!! TAATTCAACAAAACATACAA+GGG - tig0022176:16169-16188 None:intergenic 20.0%
!! TTAATTCAACAAAACATACA+AGG - tig0022176:16170-16189 None:intergenic 20.0%
!!! TTGTATGTTTTGTTGAATTA+AGG + tig0022176:16169-16188 Msa1397660:intron 20.0%
!!! TGTATGTTTTGTTGAATTAA+GGG + tig0022176:16170-16189 Msa1397660:intron 20.0%
!! AATAGATAAGCTTTCAAAAA+TGG + tig0022176:16595-16614 Msa1397660:intron 20.0%
!!! TTTTTTTATCTGCAAACTAT+AGG + tig0022176:16639-16658 Msa1397660:intron 20.0%
!! AGATTAAATGTTATGATTTC+AGG + tig0022176:16752-16771 Msa1397660:intron 20.0%
!! GATTAAATGTTATGATTTCA+GGG + tig0022176:16753-16772 Msa1397660:intron 20.0%
!!! CATTTTCAGCAAAAATAATT+AGG - tig0022176:16815-16834 None:intergenic 20.0%
!!! CATTTTCAGCAAAAATAATT+AGG - tig0022176:16815-16834 None:intergenic 20.0%
!!! ATTTTTGCTTTTGTTGTTTA+TGG + tig0022176:17577-17596 Msa1397660:three_prime_UTR 20.0%
!!! ATAATTTGTTTAGCGTATTT+TGG + tig0022176:17691-17710 Msa1397660:three_prime_UTR 20.0%
!!! TTTTGAGTGAAATACAAACA+TGG + tig0022176:15860-15879 Msa1397660:intron 25.0%
! TTTGAGTGAAATACAAACAT+GGG + tig0022176:15861-15880 Msa1397660:intron 25.0%
!! TTTTCATAAGCTCAAAAACA+AGG - tig0022176:16209-16228 None:intergenic 25.0%
!! TCTAAACAGAAAACACTTTT+AGG - tig0022176:16353-16372 None:intergenic 25.0%
!!! ATTTGTTGTTATGTATATGC+TGG + tig0022176:17064-17083 Msa1397660:intron 25.0%
! TATTCAACACAAAAAACTCT+GGG - tig0022176:17663-17682 None:intergenic 25.0%
!!! TTTTGAGTGAAATACAAACA+TGG + tig0022176:15860-15879 Msa1397660:intron 25.0%
! TTTGAGTGAAATACAAACAT+GGG + tig0022176:15861-15880 Msa1397660:intron 25.0%
!! TTTTCATAAGCTCAAAAACA+AGG - tig0022176:16209-16228 None:intergenic 25.0%
!! TCTAAACAGAAAACACTTTT+AGG - tig0022176:16353-16372 None:intergenic 25.0%
!!! ATTTGTTGTTATGTATATGC+TGG + tig0022176:17064-17083 Msa1397660:intron 25.0%
! TATTCAACACAAAAAACTCT+GGG - tig0022176:17663-17682 None:intergenic 25.0%
!! TGAAGCTTTGAACTGAATTA+TGG + tig0022176:15802-15821 Msa1397660:intron 30.0%
!!! AAATGAAGCTTGTTTTGTGA+TGG + tig0022176:15900-15919 Msa1397660:intron 30.0%
!!! AATGAAGCTTGTTTTGTGAT+GGG + tig0022176:15901-15920 Msa1397660:intron 30.0%
!! TGGGTTTTGTAATCGAAATT+AGG + tig0022176:15920-15939 Msa1397660:intron 30.0%
!! GGGTTTTGTAATCGAAATTA+GGG + tig0022176:15921-15940 Msa1397660:intron 30.0%
!! TTTCTGAAATCTTATGGTGT+AGG + tig0022176:16034-16053 Msa1397660:intron 30.0%
AAATCCTACACCACATTATT+GGG - tig0022176:16095-16114 None:intergenic 30.0%
TAAATCCTACACCACATTAT+TGG - tig0022176:16096-16115 None:intergenic 30.0%
! TAGTTTTTATCATGCTCCTA+AGG + tig0022176:16135-16154 Msa1397660:intron 30.0%
ACAAACTTCTTGAATCCATT+AGG - tig0022176:17274-17293 None:intergenic 30.0%
! TGTTGTTTATGGTATGAACA+TGG + tig0022176:17588-17607 Msa1397660:three_prime_UTR 30.0%
GTATTCAACACAAAAAACTC+TGG - tig0022176:17664-17683 None:intergenic 30.0%
!! TGAAGCTTTGAACTGAATTA+TGG + tig0022176:15802-15821 Msa1397660:intron 30.0%
!!! AAATGAAGCTTGTTTTGTGA+TGG + tig0022176:15900-15919 Msa1397660:intron 30.0%
!!! AATGAAGCTTGTTTTGTGAT+GGG + tig0022176:15901-15920 Msa1397660:intron 30.0%
!! TGGGTTTTGTAATCGAAATT+AGG + tig0022176:15920-15939 Msa1397660:intron 30.0%
!! GGGTTTTGTAATCGAAATTA+GGG + tig0022176:15921-15940 Msa1397660:intron 30.0%
!! TTTCTGAAATCTTATGGTGT+AGG + tig0022176:16034-16053 Msa1397660:intron 30.0%
AAATCCTACACCACATTATT+GGG - tig0022176:16095-16114 None:intergenic 30.0%
TAAATCCTACACCACATTAT+TGG - tig0022176:16096-16115 None:intergenic 30.0%
! TAGTTTTTATCATGCTCCTA+AGG + tig0022176:16135-16154 Msa1397660:intron 30.0%
ACAAACTTCTTGAATCCATT+AGG - tig0022176:17274-17293 None:intergenic 30.0%
! TGTTGTTTATGGTATGAACA+TGG + tig0022176:17588-17607 Msa1397660:three_prime_UTR 30.0%
GTATTCAACACAAAAAACTC+TGG - tig0022176:17664-17683 None:intergenic 30.0%
AGAGATGTAAGAAGCAAATG+AGG - tig0022176:15608-15627 None:intergenic 35.0%
TTGTTCTTATTCTCTCGATC+TGG + tig0022176:15767-15786 Msa1397660:intron 35.0%
! TCTCGATCTGGTTTCATAAA+AGG + tig0022176:15779-15798 Msa1397660:intron 35.0%
!!! ACATGGGTCGAATTGTTTTT+TGG + tig0022176:15877-15896 Msa1397660:intron 35.0%
TTATCATGCTCCCAATAATG+TGG + tig0022176:16082-16101 Msa1397660:intron 35.0%
!! GTTGAATTAAGGGCTGTTTT+AGG + tig0022176:16180-16199 Msa1397660:intron 35.0%
TGACAGGAAAATATGTGTGA+AGG + tig0022176:16564-16583 Msa1397660:CDS 35.0%
AGGTACATGCGTGTAATAAA+TGG - tig0022176:16795-16814 None:intergenic 35.0%
TGCATAGCACAACAGTAATA+AGG - tig0022176:17136-17155 None:intergenic 35.0%
CGAGTTAGGAGAAAATTCAA+AGG + tig0022176:17190-17209 Msa1397660:CDS 35.0%
TTGATGCCTAACATTGGATA+CGG + tig0022176:17220-17239 Msa1397660:CDS 35.0%
TGAATCCATTAGGCAAATAG+TGG - tig0022176:17264-17283 None:intergenic 35.0%
TGTGTCAACCAGAAAAAGAA+AGG + tig0022176:17453-17472 Msa1397660:CDS 35.0%
! AACGATCTCCTTTCTTTTTC+TGG - tig0022176:17464-17483 None:intergenic 35.0%
TTCTCAGGATTGTTGTCTAA+TGG + tig0022176:17621-17640 Msa1397660:three_prime_UTR 35.0%
AGTCACAAATCAGAAACAAG+CGG - tig0022176:17801-17820 None:intergenic 35.0%
AGAGATGTAAGAAGCAAATG+AGG - tig0022176:15608-15627 None:intergenic 35.0%
TTGTTCTTATTCTCTCGATC+TGG + tig0022176:15767-15786 Msa1397660:intron 35.0%
! TCTCGATCTGGTTTCATAAA+AGG + tig0022176:15779-15798 Msa1397660:intron 35.0%
!!! ACATGGGTCGAATTGTTTTT+TGG + tig0022176:15877-15896 Msa1397660:intron 35.0%
TTATCATGCTCCCAATAATG+TGG + tig0022176:16082-16101 Msa1397660:intron 35.0%
!! GTTGAATTAAGGGCTGTTTT+AGG + tig0022176:16180-16199 Msa1397660:intron 35.0%
TGACAGGAAAATATGTGTGA+AGG + tig0022176:16564-16583 Msa1397660:CDS 35.0%
AGGTACATGCGTGTAATAAA+TGG - tig0022176:16795-16814 None:intergenic 35.0%
TGCATAGCACAACAGTAATA+AGG - tig0022176:17136-17155 None:intergenic 35.0%
CGAGTTAGGAGAAAATTCAA+AGG + tig0022176:17190-17209 Msa1397660:CDS 35.0%
TTGATGCCTAACATTGGATA+CGG + tig0022176:17220-17239 Msa1397660:CDS 35.0%
TGAATCCATTAGGCAAATAG+TGG - tig0022176:17264-17283 None:intergenic 35.0%
TGTGTCAACCAGAAAAAGAA+AGG + tig0022176:17453-17472 Msa1397660:CDS 35.0%
! AACGATCTCCTTTCTTTTTC+TGG - tig0022176:17464-17483 None:intergenic 35.0%
TTCTCAGGATTGTTGTCTAA+TGG + tig0022176:17621-17640 Msa1397660:three_prime_UTR 35.0%
AGTCACAAATCAGAAACAAG+CGG - tig0022176:17801-17820 None:intergenic 35.0%
TCAGAAGCAGCATCGTATAA+AGG - tig0022176:15722-15741 None:intergenic 40.0%
AAGCTAAACCAGAACACACA+AGG - tig0022176:15952-15971 None:intergenic 40.0%
TATCATGCTCCTAAGGAGTA+AGG + tig0022176:16142-16161 Msa1397660:intron 40.0%
TGTGTCTTGTAGAGACAAGA+TGG + tig0022176:16477-16496 Msa1397660:intron 40.0%
ACAATCTTCTTGGTGAGCAA+TGG - tig0022176:16508-16527 None:intergenic 40.0%
ACGCTTCTTCACAATCTTCT+TGG - tig0022176:16518-16537 None:intergenic 40.0%
! CATATTTTCCTGTCACTCTG+TGG - tig0022176:16559-16578 None:intergenic 40.0%
AAAGCTCAAACATACCACGA+AGG - tig0022176:16665-16684 None:intergenic 40.0%
TAACAGGTATCAGTAGCAGT+AGG - tig0022176:17020-17039 None:intergenic 40.0%
ACTCGTGAATCAATACCCTT+TGG - tig0022176:17175-17194 None:intergenic 40.0%
!! AGGGTATTGATTCACGAGTT+AGG + tig0022176:17176-17195 Msa1397660:CDS 40.0%
GTGACTTTGATGCCTAACAT+TGG + tig0022176:17214-17233 Msa1397660:CDS 40.0%
ATCCATTAGGCAAATAGTGG+CGG - tig0022176:17261-17280 None:intergenic 40.0%
! AGCTTTTGTTGATGCACAAC+AGG + tig0022176:17317-17336 Msa1397660:CDS 40.0%
AGTTACGAAGAGACAATGAG+AGG - tig0022176:17375-17394 None:intergenic 40.0%
CATTTACATGAACAACCCAC+AGG + tig0022176:17404-17423 Msa1397660:intron 40.0%
!! GCTTGTTTCTGATTTGTGAC+TGG + tig0022176:17800-17819 Msa1397660:three_prime_UTR 40.0%
TCAGAAGCAGCATCGTATAA+AGG - tig0022176:15722-15741 None:intergenic 40.0%
AAGCTAAACCAGAACACACA+AGG - tig0022176:15952-15971 None:intergenic 40.0%
TATCATGCTCCTAAGGAGTA+AGG + tig0022176:16142-16161 Msa1397660:intron 40.0%
TGTGTCTTGTAGAGACAAGA+TGG + tig0022176:16477-16496 Msa1397660:intron 40.0%
ACAATCTTCTTGGTGAGCAA+TGG - tig0022176:16508-16527 None:intergenic 40.0%
ACGCTTCTTCACAATCTTCT+TGG - tig0022176:16518-16537 None:intergenic 40.0%
! CATATTTTCCTGTCACTCTG+TGG - tig0022176:16559-16578 None:intergenic 40.0%
AAAGCTCAAACATACCACGA+AGG - tig0022176:16665-16684 None:intergenic 40.0%
TAACAGGTATCAGTAGCAGT+AGG - tig0022176:17020-17039 None:intergenic 40.0%
ACTCGTGAATCAATACCCTT+TGG - tig0022176:17175-17194 None:intergenic 40.0%
!! AGGGTATTGATTCACGAGTT+AGG + tig0022176:17176-17195 Msa1397660:CDS 40.0%
GTGACTTTGATGCCTAACAT+TGG + tig0022176:17214-17233 Msa1397660:CDS 40.0%
ATCCATTAGGCAAATAGTGG+CGG - tig0022176:17261-17280 None:intergenic 40.0%
! AGCTTTTGTTGATGCACAAC+AGG + tig0022176:17317-17336 Msa1397660:CDS 40.0%
AGTTACGAAGAGACAATGAG+AGG - tig0022176:17375-17394 None:intergenic 40.0%
CATTTACATGAACAACCCAC+AGG + tig0022176:17404-17423 Msa1397660:intron 40.0%
!! GCTTGTTTCTGATTTGTGAC+TGG + tig0022176:17800-17819 Msa1397660:three_prime_UTR 40.0%
TGCTCCCAATAATGTGGTGT+AGG + tig0022176:16088-16107 Msa1397660:intron 45.0%
TACAAGGGTCCTTACTCCTT+AGG - tig0022176:16154-16173 None:intergenic 45.0%
CTTCTTGGTGAGCAATGGAA+CGG - tig0022176:16503-16522 None:intergenic 45.0%
! TTGTGAAGAAGCGTGTCAAG+AGG + tig0022176:16524-16543 Msa1397660:CDS 45.0%
AGGTATCAGTAGCAGTAGGT+AGG - tig0022176:17016-17035 None:intergenic 45.0%
TCTGAGCCGTATCCAATGTT+AGG - tig0022176:17229-17248 None:intergenic 45.0%
TCCATTAGGCAAATAGTGGC+GGG - tig0022176:17260-17279 None:intergenic 45.0%
TGTGCATCAACAAAAGCTCC+AGG - tig0022176:17315-17334 None:intergenic 45.0%
GTTGATGCACAACAGGTAAC+TGG + tig0022176:17324-17343 Msa1397660:intron 45.0%
AAAGGAGATCGTTGAACGTG+CGG + tig0022176:17471-17490 Msa1397660:CDS 45.0%
TGTTGTTGTCACCAACAAGC+TGG + tig0022176:17504-17523 Msa1397660:CDS 45.0%
CATGGCAGACAATTGTTCTC+AGG + tig0022176:17606-17625 Msa1397660:three_prime_UTR 45.0%
TGCTCCCAATAATGTGGTGT+AGG + tig0022176:16088-16107 Msa1397660:intron 45.0%
TACAAGGGTCCTTACTCCTT+AGG - tig0022176:16154-16173 None:intergenic 45.0%
CTTCTTGGTGAGCAATGGAA+CGG - tig0022176:16503-16522 None:intergenic 45.0%
! TTGTGAAGAAGCGTGTCAAG+AGG + tig0022176:16524-16543 Msa1397660:CDS 45.0%
AGGTATCAGTAGCAGTAGGT+AGG - tig0022176:17016-17035 None:intergenic 45.0%
TCTGAGCCGTATCCAATGTT+AGG - tig0022176:17229-17248 None:intergenic 45.0%
TCCATTAGGCAAATAGTGGC+GGG - tig0022176:17260-17279 None:intergenic 45.0%
TGTGCATCAACAAAAGCTCC+AGG - tig0022176:17315-17334 None:intergenic 45.0%
GTTGATGCACAACAGGTAAC+TGG + tig0022176:17324-17343 Msa1397660:intron 45.0%
AAAGGAGATCGTTGAACGTG+CGG + tig0022176:17471-17490 Msa1397660:CDS 45.0%
TGTTGTTGTCACCAACAAGC+TGG + tig0022176:17504-17523 Msa1397660:CDS 45.0%
CATGGCAGACAATTGTTCTC+AGG + tig0022176:17606-17625 Msa1397660:three_prime_UTR 45.0%
! TCTGAAGCAGCGATTCAAGC+AGG + tig0022176:15625-15644 Msa1397660:five_prime_UTR 50.0%
AGCGTGTCAAGAGGTTCATC+AGG + tig0022176:16533-16552 Msa1397660:CDS 50.0%
CTGCAAACTATAGGCCTTCG+TGG + tig0022176:16648-16667 Msa1397660:intron 50.0%
GTTGTGCTATGCAGCAAAGC+TGG + tig0022176:17143-17162 Msa1397660:intron 50.0%
ACCCGCCACTATTTGCCTAA+TGG + tig0022176:17256-17275 Msa1397660:CDS 50.0%
GTTTGTCGTGCACAATGTCC+AGG + tig0022176:17288-17307 Msa1397660:CDS 50.0%
TCAACAAAAGCTCCAGGTCC+TGG - tig0022176:17309-17328 None:intergenic 50.0%
CTCAGCACAGTATGTCCTGT+GGG - tig0022176:17422-17441 None:intergenic 50.0%
TCTCAGCACAGTATGTCCTG+TGG - tig0022176:17423-17442 None:intergenic 50.0%
! TCTGAAGCAGCGATTCAAGC+AGG + tig0022176:15625-15644 Msa1397660:five_prime_UTR 50.0%
AGCGTGTCAAGAGGTTCATC+AGG + tig0022176:16533-16552 Msa1397660:CDS 50.0%
CTGCAAACTATAGGCCTTCG+TGG + tig0022176:16648-16667 Msa1397660:intron 50.0%
GTTGTGCTATGCAGCAAAGC+TGG + tig0022176:17143-17162 Msa1397660:intron 50.0%
ACCCGCCACTATTTGCCTAA+TGG + tig0022176:17256-17275 Msa1397660:CDS 50.0%
GTTTGTCGTGCACAATGTCC+AGG + tig0022176:17288-17307 Msa1397660:CDS 50.0%
TCAACAAAAGCTCCAGGTCC+TGG - tig0022176:17309-17328 None:intergenic 50.0%
CTCAGCACAGTATGTCCTGT+GGG - tig0022176:17422-17441 None:intergenic 50.0%
TCTCAGCACAGTATGTCCTG+TGG - tig0022176:17423-17442 None:intergenic 50.0%
! GGGTTGAGCCTTGTGTGTTC+TGG + tig0022176:15941-15960 Msa1397660:intron 55.0%
TCATCAGGCCACAGAGTGAC+AGG + tig0022176:16548-16567 Msa1397660:CDS 55.0%
CGTTGAACGTGCGGCACAAT+TGG + tig0022176:17480-17499 Msa1397660:CDS 55.0%
TTGTCACCAACAAGCTGGCC+AGG + tig0022176:17509-17528 Msa1397660:CDS 55.0%
GTCTTCTTGACTGCGCAACC+TGG - tig0022176:17530-17549 None:intergenic 55.0%
! GGGTTGAGCCTTGTGTGTTC+TGG + tig0022176:15941-15960 Msa1397660:intron 55.0%
TCATCAGGCCACAGAGTGAC+AGG + tig0022176:16548-16567 Msa1397660:CDS 55.0%
CGTTGAACGTGCGGCACAAT+TGG + tig0022176:17480-17499 Msa1397660:CDS 55.0%
TTGTCACCAACAAGCTGGCC+AGG + tig0022176:17509-17528 Msa1397660:CDS 55.0%
GTCTTCTTGACTGCGCAACC+TGG - tig0022176:17530-17549 None:intergenic 55.0%
CTATGCAGCAAAGCTGGCGC+AGG + tig0022176:17149-17168 Msa1397660:intron 60.0%
CAAAGCTGGCGCAGGCCAAA+GGG + tig0022176:17157-17176 Msa1397660:CDS 60.0%
CGTGCACAATGTCCAGGACC+TGG + tig0022176:17294-17313 Msa1397660:CDS 60.0%
CTATGCAGCAAAGCTGGCGC+AGG + tig0022176:17149-17168 Msa1397660:intron 60.0%
CAAAGCTGGCGCAGGCCAAA+GGG + tig0022176:17157-17176 Msa1397660:CDS 60.0%
CGTGCACAATGTCCAGGACC+TGG + tig0022176:17294-17313 Msa1397660:CDS 60.0%
GCAAAGCTGGCGCAGGCCAA+AGG + tig0022176:17156-17175 Msa1397660:intron 65.0%
GCGCAACCTGGCCAGCTTGT+TGG - tig0022176:17518-17537 None:intergenic 65.0%
GCAAAGCTGGCGCAGGCCAA+AGG + tig0022176:17156-17175 Msa1397660:intron 65.0%
GCGCAACCTGGCCAGCTTGT+TGG - tig0022176:17518-17537 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0022176 gene 15600 17852 15600 ID=Msa1397660;Name=Msa1397660
tig0022176 mRNA 15600 17852 15600 ID=Msa1397660-mRNA-1;Parent=Msa1397660;Name=Msa1397660-mRNA-1;_AED=0.06;_eAED=0.06;_QI=54|1|1|1|0.66|0.75|4|297|133
tig0022176 exon 15600 15646 15600 ID=Msa1397660-mRNA-1:exon:16227;Parent=Msa1397660-mRNA-1
tig0022176 exon 16489 16585 16489 ID=Msa1397660-mRNA-1:exon:16228;Parent=Msa1397660-mRNA-1
tig0022176 exon 17157 17338 17157 ID=Msa1397660-mRNA-1:exon:16229;Parent=Msa1397660-mRNA-1
tig0022176 exon 17426 17852 17426 ID=Msa1397660-mRNA-1:exon:16230;Parent=Msa1397660-mRNA-1
tig0022176 five_prime_UTR 15600 15646 15600 ID=Msa1397660-mRNA-1:five_prime_utr;Parent=Msa1397660-mRNA-1
tig0022176 five_prime_UTR 16489 16495 16489 ID=Msa1397660-mRNA-1:five_prime_utr;Parent=Msa1397660-mRNA-1
tig0022176 CDS 16496 16585 16496 ID=Msa1397660-mRNA-1:cds;Parent=Msa1397660-mRNA-1
tig0022176 CDS 17157 17338 17157 ID=Msa1397660-mRNA-1:cds;Parent=Msa1397660-mRNA-1
tig0022176 CDS 17426 17555 17426 ID=Msa1397660-mRNA-1:cds;Parent=Msa1397660-mRNA-1
tig0022176 three_prime_UTR 17556 17852 17556 ID=Msa1397660-mRNA-1:three_prime_utr;Parent=Msa1397660-mRNA-1
Gene Sequence

>Msa1397660

ATGGCCGTTCCATTGCTCACCAAGAAGATTGTGAAGAAGCGTGTCAAGAGGTTCATCAGGCCACAGAGTGACAGGAAAATATGTGTGAAGCAAAGCTGGCGCAGGCCAAAGGGTATTGATTCACGAGTTAGGAGAAAATTCAAAGGAGTGACTTTGATGCCTAACATTGGATACGGCTCAGACAAGAAGACCCGCCACTATTTGCCTAATGGATTCAAGAAGTTTGTCGTGCACAATGTCCAGGACCTGGAGCTTTTGTTGATGCACAACAGGACATACTGTGCTGAGATTGCACACAATGTGTCAACCAGAAAAAGAAAGGAGATCGTTGAACGTGCGGCACAATTGGATGTTGTTGTCACCAACAAGCTGGCCAGGTTGCGCAGTCAAGAAGACGAGTGA
Protein sequence

>Msa1397660

MAVPLLTKKIVKKRVKRFIRPQSDRKICVKQSWRRPKGIDSRVRRKFKGVTLMPNIGYGSDKKTRHYLPNGFKKFVVHNVQDLELLLMHNRTYCAEIAHNVSTRKRKEIVERAAQLDVVVTNKLARLRSQEDE*