AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1162040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1162040 MtrunA17_Chr7g0257721 93.976 332 19 1 63 393 1 332 0.0 615
Msa1162040 MtrunA17_Chr1g0173451 63.616 448 146 9 63 505 1 436 0.0 546
Msa1162040 MtrunA17_Chr7g0257701 68.796 407 74 5 63 433 1 390 0.0 520
Msa1162040 MtrunA17_Chr4g0020771 49.234 457 208 8 59 507 4 444 3.12e-142 416
Msa1162040 MtrunA17_Chr3g0089021 49.443 449 209 5 63 505 1 437 2.84e-135 398
Msa1162040 MtrunA17_Chr2g0332801 48.509 369 187 3 136 502 58 425 6.14e-118 354
Msa1162040 MtrunA17_Chr8g0365901 45.924 368 196 3 136 502 64 429 1.57e-116 351
Msa1162040 MtrunA17_Chr6g0449961 44.536 366 185 6 122 477 21 378 2.57e-99 306
Msa1162040 MtrunA17_Chr4g0052621 43.526 363 192 6 131 483 59 418 8.80e-93 290
Msa1162040 MtrunA17_Chr1g0194231 40.833 360 198 6 136 483 80 436 1.64e-89 282
Msa1162040 MtrunA17_Chr5g0413411 40.108 369 191 5 136 477 120 485 1.45e-86 276
Msa1162040 MtrunA17_Chr7g0270361 40.111 359 201 6 136 483 66 421 7.12e-86 272
Msa1162040 MtrunA17_Chr4g0043431 43.902 369 197 9 138 503 36 397 9.92e-86 270
Msa1162040 MtrunA17_Chr2g0332971 41.329 346 195 6 138 477 29 372 6.37e-83 263
Msa1162040 MtrunA17_Chr4g0043661 39.370 381 206 7 138 507 24 390 3.59e-82 260
Msa1162040 MtrunA17_Chr2g0303941 40.541 370 208 7 137 503 28 388 8.51e-82 259
Msa1162040 MtrunA17_Chr5g0413141 39.595 346 200 4 138 477 60 402 1.24e-80 258
Msa1162040 MtrunA17_Chr8g0365891 37.736 371 194 5 138 477 108 472 2.68e-80 259
Msa1162040 MtrunA17_Chr1g0195221 37.084 391 223 10 128 502 19 402 2.12e-79 254
Msa1162040 MtrunA17_Chr8g0348981 41.880 351 189 8 135 477 25 368 3.75e-79 253
Msa1162040 MtrunA17_Chr7g0228411 38.035 397 213 10 124 493 27 417 2.09e-78 252
Msa1162040 MtrunA17_Chr7g0257711 98.305 118 2 0 394 511 3 120 2.99e-78 240
Msa1162040 MtrunA17_Chr4g0067631 39.628 376 212 10 135 502 50 418 6.90e-78 250
Msa1162040 MtrunA17_Chr5g0418411 39.362 376 214 5 124 493 16 383 9.36e-78 249
Msa1162040 MtrunA17_Chr3g0121881 39.237 367 208 6 135 495 31 388 1.79e-77 248
Msa1162040 MtrunA17_Chr3g0121891 39.840 374 204 8 127 489 15 378 4.92e-77 247
Msa1162040 MtrunA17_Chr4g0018151 39.837 369 205 5 130 493 71 427 9.92e-77 248
Msa1162040 MtrunA17_Chr2g0293681 42.683 328 176 4 136 454 66 390 2.18e-76 246
Msa1162040 MtrunA17_Chr7g0230891 40.108 369 209 8 138 503 38 397 2.48e-76 246
Msa1162040 MtrunA17_Chr8g0337571 41.209 364 190 9 136 493 30 375 4.26e-76 244
Msa1162040 MtrunA17_Chr2g0295451 42.623 305 163 8 135 430 51 352 7.70e-74 238
Msa1162040 MtrunA17_Chr8g0337581 39.726 365 195 11 136 493 30 376 4.08e-73 237
Msa1162040 MtrunA17_Chr1g0150291 36.119 371 224 8 137 503 45 406 1.56e-69 228
Msa1162040 MtrunA17_Chr6g0461181 36.228 403 196 9 138 492 31 420 1.82e-69 229
Msa1162040 MtrunA17_Chr2g0307811 36.508 378 225 9 136 506 24 393 3.18e-69 227
Msa1162040 MtrunA17_Chr1g0150301 37.119 361 214 8 137 493 27 378 7.69e-68 223
Msa1162040 MtrunA17_Chr2g0291841 40.303 330 184 9 179 503 38 359 1.65e-65 216
Msa1162040 MtrunA17_Chr2g0307831 33.422 377 238 8 136 506 24 393 3.47e-63 212
Msa1162040 MtrunA17_Chr2g0307821 33.422 377 238 8 136 506 24 393 3.47e-63 212
Msa1162040 MtrunA17_Chr3g0100091 36.658 371 181 6 138 507 31 348 2.02e-62 207
Msa1162040 MtrunA17_Chr5g0447901 38.356 292 172 5 136 423 24 311 1.35e-60 202
Msa1162040 MtrunA17_Chr1g0149241 34.332 367 193 9 133 493 24 348 1.04e-56 193
Msa1162040 MtrunA17_Chr5g0425811 36.727 275 159 7 154 423 2 266 1.79e-53 182
Msa1162040 MtrunA17_Chr8g0336481 39.925 268 145 4 196 461 14 267 6.14e-52 177
Msa1162040 MtrunA17_Chr8g0337521 40.530 264 133 8 136 393 30 275 1.15e-49 172
Msa1162040 MtrunA17_Chr8g0337511 41.152 243 134 3 262 503 5 239 1.65e-49 170
Msa1162040 MtrunA17_Chr3g0093011 40.650 246 141 3 261 502 13 257 1.18e-48 168
Msa1162040 MtrunA17_Chr8g0337601 43.777 233 123 4 262 493 2 227 8.33e-48 166
Msa1162040 MtrunA17_Chr7g0273191 54.015 137 63 0 239 375 2 138 9.67e-44 151
Msa1162040 MtrunA17_Chr8g0337351 49.045 157 79 1 272 427 1 157 4.14e-37 135
Msa1162040 MtrunA17_Chr8g0337551 50.000 130 65 0 264 393 7 136 1.29e-29 113
Msa1162040 MtrunA17_Chr3g0093021 40.476 126 69 5 131 254 21 142 4.18e-22 93.6
Msa1162040 MtrunA17_Chr4g0070281 24.409 381 228 14 136 475 74 435 3.14e-20 93.6
Msa1162040 MtrunA17_Chr8g0337541 37.500 120 66 6 136 249 30 146 1.87e-18 82.4
Msa1162040 MtrunA17_Chr7g0273181 44.792 96 50 2 409 502 1 95 1.96e-18 81.3
Msa1162040 MtrunA17_Chr5g0442221 24.771 327 193 13 137 425 44 355 1.13e-17 85.5
Msa1162040 MtrunA17_Chr2g0295241 25.183 409 242 14 137 503 37 423 2.43e-17 84.3
Msa1162040 MtrunA17_Chr3g0130251 25.248 404 204 15 136 477 75 442 2.08e-16 81.6
Msa1162040 MtrunA17_Chr7g0248451 23.956 455 256 18 108 505 24 445 2.22e-16 81.6
Msa1162040 MtrunA17_Chr1g0202641 25.067 375 230 14 139 477 57 416 2.41e-15 79.3
Msa1162040 MtrunA17_Chr1g0202641 25.806 403 242 16 139 502 557 941 1.49e-14 76.6
Msa1162040 MtrunA17_Chr2g0330221 26.875 160 109 4 153 309 39 193 5.03e-15 73.9
Msa1162040 MtrunA17_Chr8g0387391 23.670 376 238 14 137 477 44 405 5.78e-15 77.4
Msa1162040 MtrunA17_Chr4g0067811 23.604 394 246 12 137 492 40 416 6.29e-15 77.0
Msa1162040 MtrunA17_Chr7g0255311 23.472 409 257 16 109 481 41 429 1.18e-14 76.3
Msa1162040 MtrunA17_Chr8g0348491 23.558 416 241 14 137 503 55 442 1.50e-14 75.9
Msa1162040 MtrunA17_Chr7g0270821 26.355 406 228 20 137 486 43 433 1.83e-14 75.5
Msa1162040 MtrunA17_Chr1g0193961 26.934 349 205 13 137 447 43 379 2.03e-14 75.5
Msa1162040 MtrunA17_Chr1g0202651 24.267 375 233 12 139 477 57 416 2.96e-14 75.1
Msa1162040 MtrunA17_Chr8g0373171 43.529 85 48 0 262 346 7 91 1.03e-13 67.8
Msa1162040 MtrunA17_Chr3g0095221 24.451 319 203 11 137 425 47 357 3.28e-13 71.6
Msa1162040 MtrunA17_Chr8g0337561 36.634 101 56 3 394 493 8 101 9.21e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1162040 AT3G07970.1 51.339 448 198 10 63 504 5 438 4.37e-145 424
Msa1162040 AT3G57510.1 49.103 446 207 8 63 504 1 430 5.36e-142 416
Msa1162040 AT2G41850.1 57.407 378 150 5 132 506 63 432 2.30e-139 409
Msa1162040 AT1G80170.1 46.524 374 196 4 132 502 48 420 2.74e-116 350
Msa1162040 AT1G70500.1 41.037 463 240 7 44 505 4 434 7.15e-116 350
Msa1162040 AT1G23460.2 47.011 368 192 3 136 502 42 407 2.61e-114 345
Msa1162040 AT1G23460.1 46.631 371 195 3 136 505 66 434 3.16e-114 345
Msa1162040 AT5G14650.1 43.567 342 186 5 137 477 51 386 1.34e-91 286
Msa1162040 AT1G56710.1 41.667 360 195 6 136 483 47 403 3.87e-90 283
Msa1162040 AT1G10640.1 41.622 370 186 5 135 477 117 483 1.40e-89 284
Msa1162040 AT1G02460.1 42.424 363 191 7 136 483 88 447 2.31e-89 283
Msa1162040 AT1G60590.1 40.541 370 187 5 138 477 124 490 1.83e-88 281
Msa1162040 AT3G15720.1 40.872 367 201 6 137 497 24 380 4.24e-86 273
Msa1162040 AT3G15720.3 40.872 367 201 6 137 497 23 379 5.04e-86 273
Msa1162040 AT4G18180.1 38.693 398 217 11 129 505 31 422 2.13e-85 270
Msa1162040 AT2G43890.1 40.108 369 209 8 138 503 30 389 2.28e-85 269
Msa1162040 AT1G80170.2 47.909 263 135 2 132 392 48 310 2.29e-85 267
Msa1162040 AT1G48100.1 40.223 358 201 5 136 483 79 433 6.35e-85 270
Msa1162040 AT3G26610.1 42.391 368 196 6 136 491 66 429 6.23e-84 268
Msa1162040 AT4G01890.1 40.650 369 195 9 125 478 59 418 1.10e-83 267
Msa1162040 AT1G05660.1 39.623 371 211 8 136 502 27 388 1.45e-83 265
Msa1162040 AT1G05650.1 39.153 378 217 8 129 502 20 388 2.25e-83 264
Msa1162040 AT2G43860.1 42.216 379 201 10 122 495 22 387 3.97e-83 264
Msa1162040 AT3G15720.2 40.054 367 201 6 137 497 23 376 5.62e-83 265
Msa1162040 AT2G43880.1 38.727 377 219 8 130 503 24 391 2.27e-82 261
Msa1162040 AT5G44840.1 38.950 362 209 5 135 492 22 375 2.60e-82 261
Msa1162040 AT2G33160.1 40.957 376 208 9 135 502 30 399 7.80e-82 268
Msa1162040 AT5G27530.1 41.322 363 192 5 135 493 51 396 1.85e-81 261
Msa1162040 AT4G35670.1 41.783 359 196 5 138 492 25 374 3.32e-81 258
Msa1162040 AT1G78400.1 38.606 373 218 7 135 502 33 399 6.39e-81 258
Msa1162040 AT1G17150.1 40.260 385 213 11 130 504 23 400 6.93e-81 258
Msa1162040 AT2G43870.1 38.845 381 218 10 129 504 13 383 1.31e-80 256
Msa1162040 AT5G17200.1 38.903 383 201 6 136 495 38 410 1.91e-80 257
Msa1162040 AT3G59850.2 40.270 370 207 10 138 502 68 428 2.32e-79 254
Msa1162040 AT3G59850.1 40.270 370 207 10 138 502 24 384 4.18e-79 253
Msa1162040 AT1G02790.1 38.120 383 210 11 136 504 51 420 1.35e-76 247
Msa1162040 AT1G43080.1 37.632 380 218 10 135 503 29 400 7.87e-76 244
Msa1162040 AT1G43090.1 37.600 375 215 10 135 498 29 395 3.37e-75 244
Msa1162040 AT4G32380.1 38.439 346 200 6 136 475 25 363 3.81e-75 244
Msa1162040 AT2G15460.1 37.532 389 221 11 129 503 20 400 6.81e-75 242
Msa1162040 AT4G13760.1 37.105 380 220 10 135 503 29 400 1.74e-74 241
Msa1162040 AT1G43100.1 37.333 375 216 10 135 498 29 395 3.71e-74 241
Msa1162040 AT2G15450.1 38.547 358 202 9 129 475 20 370 3.01e-73 238
Msa1162040 AT3G07840.1 35.340 382 229 7 137 510 21 392 1.14e-72 236
Msa1162040 AT2G40310.1 36.412 379 224 9 135 503 29 400 4.72e-72 235
Msa1162040 AT2G15470.1 37.989 358 204 9 129 475 20 370 2.02e-71 233
Msa1162040 AT1G65570.1 38.978 372 212 11 137 502 31 393 2.26e-71 233
Msa1162040 AT3G15720.4 39.815 324 180 5 179 497 3 316 4.02e-71 232
Msa1162040 AT3G07830.1 36.813 364 215 9 150 506 34 389 4.24e-70 229
Msa1162040 AT3G14040.1 38.070 373 215 10 137 502 71 434 1.02e-68 227
Msa1162040 AT2G26620.1 36.693 387 225 11 129 503 20 398 1.21e-68 226
Msa1162040 AT5G44830.1 38.024 334 185 5 163 492 1 316 1.54e-68 223
Msa1162040 AT2G43880.2 38.535 314 183 6 130 440 24 330 1.60e-68 223
Msa1162040 AT3G07820.1 36.212 359 217 7 150 503 33 384 2.00e-68 225
Msa1162040 AT5G48140.1 35.509 383 226 8 137 510 21 391 3.51e-68 224
Msa1162040 AT1G80140.1 37.910 335 193 7 174 502 7 332 5.28e-68 222
Msa1162040 AT3G07850.1 37.802 373 216 10 137 502 70 433 9.04e-68 225
Msa1162040 AT5G14650.2 43.191 257 142 3 137 392 51 304 9.69e-68 221
Msa1162040 AT4G32370.1 41.639 305 159 9 135 428 32 328 7.85e-66 216
Msa1162040 AT4G32380.2 34.493 345 174 6 136 475 25 322 2.04e-61 207
Msa1162040 AT4G32375.1 40.840 262 148 1 214 475 9 263 2.09e-61 207
Msa1162040 AT4G35670.2 42.279 272 148 2 218 489 2 264 2.91e-60 200
Msa1162040 AT5G39910.1 36.513 304 166 6 136 433 35 317 3.66e-58 197
Msa1162040 AT1G19170.1 23.671 414 247 16 136 503 86 476 4.56e-20 93.6
Msa1162040 AT3G42950.1 24.672 381 227 15 136 475 65 426 2.85e-18 88.2
Msa1162040 AT3G62110.1 27.073 410 246 21 137 508 43 437 4.67e-17 84.3
Msa1162040 AT3G62110.2 27.073 410 246 21 137 508 43 437 4.67e-17 84.3
Msa1162040 AT4G23820.1 27.063 303 185 9 137 410 41 336 4.97e-17 84.0
Msa1162040 AT4G33440.1 23.645 406 210 14 136 470 71 447 2.16e-16 82.0
Msa1162040 AT3G06770.2 25.849 383 217 12 137 475 43 402 4.54e-16 80.9
Msa1162040 AT3G06770.5 25.849 383 217 12 137 475 44 403 5.08e-16 80.9
Msa1162040 AT2G23900.1 24.752 404 248 17 139 503 58 444 4.22e-14 75.1
Msa1162040 AT5G41870.1 25.312 320 202 10 135 424 43 355 7.21e-14 74.3
Msa1162040 AT3G61490.4 23.325 403 258 15 137 503 158 545 5.25e-13 72.0
Msa1162040 AT3G61490.5 23.325 403 258 15 137 503 60 447 6.94e-13 71.2
Msa1162040 AT3G61490.2 23.325 403 258 15 137 503 45 432 1.00e-12 70.9
Msa1162040 AT3G61490.3 23.325 403 258 15 137 503 45 432 1.00e-12 70.9
Msa1162040 AT3G61490.1 23.325 403 258 15 137 503 45 432 1.00e-12 70.9
Msa1162040 AT4G23500.1 24.257 404 253 18 137 503 68 455 1.02e-12 70.9
Msa1162040 AT3G06770.4 26.199 271 169 7 223 475 58 315 2.55e-12 68.9
Msa1162040 AT3G06770.1 26.199 271 169 7 223 475 76 333 4.64e-12 68.2
Msa1162040 AT3G06770.3 26.199 271 169 7 223 475 76 333 4.64e-12 68.2

Find 116 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTACAACACATTGCTAATT+TGG 0.092906 7_4:+52426600 None:intergenic
GTGAATGGAGCTAAGATTTC+TGG 0.145479 7_4:-52427769 Msa1162040:CDS
GTAATTCTGATTAATTATTA+TGG 0.180204 7_4:+52426783 None:intergenic
TCGATTAATGTTAAAGCTTC+TGG 0.232871 7_4:-52428281 Msa1162040:CDS
TGGGAAGTAGTATGTGATTC+AGG 0.267912 7_4:-52429120 Msa1162040:CDS
AAATTTAATTGTCAGATATC+TGG 0.291470 7_4:-52428901 Msa1162040:intron
GTTCAGCAACTATTAGTTAA+TGG 0.294863 7_4:-52428808 Msa1162040:CDS
AAATCCAAGGACATCGTTTC+GGG 0.299515 7_4:-52427799 Msa1162040:CDS
ACTACAAATTAATGTTCAAT+TGG 0.300538 7_4:+52426280 None:intergenic
TAATTCTGATTAATTATTAT+GGG 0.319605 7_4:+52426784 None:intergenic
ATTGTTAATGTCAAAGATTA+TGG 0.321747 7_4:-52429496 Msa1162040:CDS
GATTAAATGCAGGGAGGTTC+AGG 0.322241 7_4:-52426859 Msa1162040:intron
TCACAGAAATATACAAATTA+AGG 0.339722 7_4:-52426395 Msa1162040:exon
CAAATCTAACATATCAGTTC+AGG 0.357768 7_4:-52429028 Msa1162040:intron
ACAAATTCACGTTTCATTCC+AGG 0.368923 7_4:-52428306 Msa1162040:CDS
TTCATATTTATATCCACATA+TGG 0.375972 7_4:+52429919 None:intergenic
ATGAGAGGAGGAATTGTCTT+AGG 0.383327 7_4:+52429707 None:intergenic
TATCTCAGTATTGGAAGCTT+GGG 0.384966 7_4:-52427829 Msa1162040:intron
CAAATTTCAGAACATTGAAA+TGG 0.394222 7_4:-52426821 Msa1162040:CDS
TTTATGGAGCAAACGTTGTA+TGG 0.398145 7_4:-52430033 None:intergenic
AAGATTTCTGGAACTACAAA+TGG 0.398447 7_4:-52427757 Msa1162040:CDS
GTGGACCAGGCCATGGCATC+AGG 0.404513 7_4:-52427954 Msa1162040:intron
ATAGGTATGGAAGTTTAGTT+AGG 0.413211 7_4:+52429954 None:intergenic
GTCAAAGATTATGGAGCTAA+AGG 0.414898 7_4:-52429487 Msa1162040:CDS
AAAATCCAAGGACATCGTTT+CGG 0.414961 7_4:-52427800 Msa1162040:CDS
CAAGGACCAGAGAATCTGAT+TGG 0.415737 7_4:+52429051 None:intergenic
AAAATCTCTAGCTCTATCAT+AGG 0.416238 7_4:-52428188 Msa1162040:CDS
TCAAAGATTATGGAGCTAAA+GGG 0.422051 7_4:-52429486 Msa1162040:CDS
GATTGTATATCAATTGTAGA+TGG 0.423588 7_4:-52428009 Msa1162040:CDS
CTGATTAATTATTATGGGGT+TGG 0.424803 7_4:+52426789 None:intergenic
ATATGAATTCCATCGGTGTT+AGG 0.428068 7_4:+52428230 None:intergenic
ATATGGATATATAGATATAT+AGG 0.430144 7_4:+52429936 None:intergenic
AAAATACAAAAGAACTATAA+TGG 0.434958 7_4:-52429734 Msa1162040:CDS
ACATGTTTCATATTGTTGTA+AGG 0.437458 7_4:-52426201 Msa1162040:three_prime_UTR
AGAGTGTGGTATAGAGACTT+TGG 0.439073 7_4:-52430000 Msa1162040:five_prime_UTR
ATATCTCAGTATTGGAAGCT+TGG 0.439364 7_4:-52427830 Msa1162040:intron
TTCAGGCATTCAAGAAGGCT+TGG 0.439440 7_4:-52429140 Msa1162040:intron
GGTCTCAATGTGTCATCGCC+AGG 0.441285 7_4:-52428260 Msa1162040:CDS
CTCAAACCAATCAGATTCTC+TGG 0.441568 7_4:-52429057 Msa1162040:CDS
GTGTTGTACCAGAATATAAC+AGG 0.441587 7_4:-52426587 Msa1162040:CDS
TACATTATTATTCAAGAACT+TGG 0.444587 7_4:-52429581 Msa1162040:CDS
TAATGTATCACCATCATCAT+AGG 0.445190 7_4:+52429597 None:intergenic
CAAACGTTGTATGGAGCAAC+AGG 0.454334 7_4:-52430024 Msa1162040:five_prime_UTR
TCTAGCTCTATCATAGGAAC+TGG 0.455625 7_4:-52428182 Msa1162040:intron
CTTTAACATTAATCGAATCC+TGG 0.456787 7_4:+52428288 None:intergenic
CTACAAATTAATGTTCAATT+GGG 0.463780 7_4:+52426281 None:intergenic
TACTCCCGAAACGATGTCCT+TGG 0.473033 7_4:+52427795 None:intergenic
TCAGGCATTCAAGAAGGCTT+GGG 0.482423 7_4:-52429139 Msa1162040:intron
TTGTTGCATAAAGCCTTAGC+AGG 0.490400 7_4:+52426461 None:intergenic
AACTACCTGATGCCATGGCC+TGG 0.490944 7_4:+52427949 None:intergenic
GAGTGTGGTATAGAGACTTT+GGG 0.495358 7_4:-52429999 Msa1162040:five_prime_UTR
TGATCTTCACTGTAATCTGA+TGG 0.496203 7_4:+52428856 None:intergenic
ATGAAACATGTCACAAGTAA+TGG 0.505301 7_4:+52426213 None:intergenic
TGTATCGAGGTATGGAACTA+AGG 0.506317 7_4:+52429842 None:intergenic
ATACTCTTAATATGTGAAGA+AGG 0.514121 7_4:-52426488 Msa1162040:CDS
GTTTCGGGAGTATTAGTGAA+TGG 0.514173 7_4:-52427784 Msa1162040:CDS
GGTGGCAAAACTCATGCAAA+AGG 0.522181 7_4:-52428753 Msa1162040:CDS
TAATATTCAGGCATTCAAGA+AGG 0.522536 7_4:-52429145 Msa1162040:intron
AGTAGATAATTATAATCTTG+AGG 0.527229 7_4:+52429078 None:intergenic
GGTGGAACCATCGATGGAAA+AGG 0.528567 7_4:-52428784 Msa1162040:CDS
CAACTATTAGTTAATGGTGG+TGG 0.535177 7_4:-52428802 Msa1162040:CDS
GGGAAGTAGTATGTGATTCA+GGG 0.539599 7_4:-52429119 Msa1162040:CDS
TATATCTATATATCCATATG+TGG 0.545137 7_4:-52429932 Msa1162040:five_prime_UTR
GATATATAGATATATAGGTA+TGG 0.548474 7_4:+52429941 None:intergenic
CAGTGAGAAATTTCCATGCA+AGG 0.551676 7_4:-52426528 Msa1162040:CDS
TGATGACGATAGTGAATGAG+AGG 0.555898 7_4:+52429692 None:intergenic
GATTATGGAGCTAAAGGGGA+TGG 0.559266 7_4:-52429481 Msa1162040:CDS
ATATATAGATGCACTTTGAA+AGG 0.559322 7_4:+52429772 None:intergenic
CAACGTATGTAGTAATAAGC+GGG 0.563528 7_4:+52429632 None:intergenic
AATTCTGATTAATTATTATG+GGG 0.565719 7_4:+52426785 None:intergenic
TCGAGGTATGGAACTAAGGA+GGG 0.569798 7_4:+52429846 None:intergenic
AGTTAGAATTAAGACTTGGC+CGG 0.569890 7_4:-52427734 Msa1162040:intron
ATTTCTGTGAGTTGCAGCGT+GGG 0.569894 7_4:+52426408 None:intergenic
ACTGATATGTTAGATTTGCA+AGG 0.570724 7_4:+52429033 None:intergenic
AAAAGCTATGTATCGAGGTA+TGG 0.571387 7_4:+52429834 None:intergenic
TCGATGGAAAAGGAAACATA+TGG 0.573459 7_4:-52428774 Msa1162040:CDS
TCTAACATTTGATAGTTGCA+AGG 0.576561 7_4:-52428366 Msa1162040:CDS
GATTCACCTTCCTATGATGA+TGG 0.579625 7_4:-52429607 Msa1162040:CDS
ATCTCAGTATTGGAAGCTTG+GGG 0.581140 7_4:-52427828 Msa1162040:intron
GCCTTGCAAGGATGCTCCAA+CGG 0.581239 7_4:-52428592 Msa1162040:intron
CAAAGATTATGGAGCTAAAG+GGG 0.581495 7_4:-52429485 Msa1162040:CDS
AATGGTGGTGGAACCATCGA+TGG 0.585351 7_4:-52428790 Msa1162040:CDS
AATGTTCAATTGTCTTACAC+AGG 0.586671 7_4:-52426440 Msa1162040:CDS
ACCGTTGGAGCATCCTTGCA+AGG 0.589497 7_4:+52428591 None:intergenic
ATTACTTGTGGACCAGGCCA+TGG 0.589996 7_4:-52427961 Msa1162040:CDS
TGAAGAAGGAGAACCTGCTA+AGG 0.593731 7_4:-52426474 Msa1162040:CDS
TTGCAATAGCAACTTGCAAG+AGG 0.593871 7_4:-52429656 Msa1162040:CDS
AGAGGAGGAATTGTCTTAGG+AGG 0.600709 7_4:+52429710 None:intergenic
ACAGACATTACTTGTGGACC+AGG 0.604491 7_4:-52427967 Msa1162040:CDS
GTCTCAATGTGTCATCGCCA+GGG 0.606055 7_4:-52428259 Msa1162040:CDS
CAGCAACTATTAGTTAATGG+TGG 0.608202 7_4:-52428805 Msa1162040:CDS
AAGTTAACTACCTGATGCCA+TGG 0.609029 7_4:+52427944 None:intergenic
GAGGTATGGAACTAAGGAGG+GGG 0.611371 7_4:+52429848 None:intergenic
GGGAATAGTCCTAACACCGA+TGG 0.616454 7_4:-52428239 Msa1162040:CDS
ATGGAGTTAGAATTAAGACT+TGG 0.618304 7_4:-52427738 Msa1162040:CDS
ATTGGTGATATGAATTCCAT+CGG 0.620342 7_4:+52428223 None:intergenic
GAAGCCACAGACATTACTTG+TGG 0.628882 7_4:-52427973 Msa1162040:CDS
TGGTCCACAAGTAATGTCTG+TGG 0.628925 7_4:+52427969 None:intergenic
ATCGAGGTATGGAACTAAGG+AGG 0.629235 7_4:+52429845 None:intergenic
TGACGATAGTGAATGAGAGG+AGG 0.634888 7_4:+52429695 None:intergenic
ACAGTGAAGATCAAAGACAT+TGG 0.636413 7_4:-52428846 Msa1162040:CDS
TCAACGTATGTAGTAATAAG+CGG 0.641060 7_4:+52429631 None:intergenic
TCGGTGTTAGGACTATTCCC+TGG 0.643264 7_4:+52428242 None:intergenic
AGATTGTGTATTTATAGACA+AGG 0.643980 7_4:+52429872 None:intergenic
GTATCACCATCATCATAGGA+AGG 0.644782 7_4:+52429601 None:intergenic
CGAGGTATGGAACTAAGGAG+GGG 0.647138 7_4:+52429847 None:intergenic
TATTTCTGTGAGTTGCAGCG+TGG 0.653112 7_4:+52426407 None:intergenic
ATGGAGCAACAGGCAGAGTG+TGG 0.668229 7_4:-52430014 Msa1162040:five_prime_UTR
TCTCAGTATTGGAAGCTTGG+GGG 0.679058 7_4:-52427827 Msa1162040:intron
TGGTCATACTGATGACACAC+AGG 0.686944 7_4:-52429461 Msa1162040:intron
ATGGAAAAGGAAACATATGG+TGG 0.687596 7_4:-52428771 Msa1162040:CDS
TAGATGCACTTTGAAAGGTG+AGG 0.687852 7_4:+52429777 None:intergenic
TTAGCAAAAGCTATGTATCG+AGG 0.688155 7_4:+52429829 None:intergenic
AGGTATGGAACTAAGGAGGG+GGG 0.695636 7_4:+52429849 None:intergenic
GTTGTTTATGCAGGACTCTG+CGG 0.702531 7_4:-52426624 Msa1162040:intron
GAGAACGTGAAGTGACACCA+TGG 0.725055 7_4:+52429524 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAACAAAAAATATCT+TGG + chr7_4:52426830-52426849 None:intergenic 10.0%
!! TTATAATTTACTTAAAAATG+TGG + chr7_4:52427267-52427286 None:intergenic 10.0%
!! AGAAATATTTATTTATTTAG+TGG + chr7_4:52429057-52429076 None:intergenic 10.0%
!! TAATTCTGATTAATTATTAT+GGG + chr7_4:52429430-52429449 None:intergenic 10.0%
!! ATATGGATATATAGATATAT+AGG + chr7_4:52426278-52426297 None:intergenic 15.0%
!! AAAATACAAAAGAACTATAA+TGG - chr7_4:52426477-52426496 Msa1162040:CDS 15.0%
!!! TTTGTTTTCAATTATAACAA+TGG - chr7_4:52426856-52426875 Msa1162040:intron 15.0%
!!! TTGTTTTCAATTATAACAAT+GGG - chr7_4:52426857-52426876 Msa1162040:intron 15.0%
!! CATACAAAATATTAAATTAG+TGG + chr7_4:52426947-52426966 None:intergenic 15.0%
!!! AACTTAAATTTTCCTATAAT+TGG - chr7_4:52427029-52427048 Msa1162040:intron 15.0%
!!! ATTGACTTGATTAAAATATT+GGG + chr7_4:52427516-52427535 None:intergenic 15.0%
!!! AATTGACTTGATTAAAATAT+TGG + chr7_4:52427517-52427536 None:intergenic 15.0%
!!! ATGAAATTCATATGTTTTTT+AGG - chr7_4:52428105-52428124 Msa1162040:intron 15.0%
!! TTAAATATAAACCAATGTAT+AGG + chr7_4:52429297-52429316 None:intergenic 15.0%
!! GGTTTATATTTAAACAAATA+AGG - chr7_4:52429304-52429323 Msa1162040:intron 15.0%
!! AATTCTGATTAATTATTATG+GGG + chr7_4:52429429-52429448 None:intergenic 15.0%
!! GTAATTCTGATTAATTATTA+TGG + chr7_4:52429431-52429450 None:intergenic 15.0%
!!! TAATTTACCATTTTTTTACA+TGG + chr7_4:52429472-52429491 None:intergenic 15.0%
!! GATATATAGATATATAGGTA+TGG + chr7_4:52426273-52426292 None:intergenic 20.0%
!! TATATCTATATATCCATATG+TGG - chr7_4:52426279-52426298 Msa1162040:three_prime_UTR 20.0%
!! TTCATATTTATATCCACATA+TGG + chr7_4:52426295-52426314 None:intergenic 20.0%
!! TACATTATTATTCAAGAACT+TGG - chr7_4:52426630-52426649 Msa1162040:intron 20.0%
!! ATTGTTAATGTCAAAGATTA+TGG - chr7_4:52426715-52426734 Msa1162040:intron 20.0%
!! TAAGAAAACCAAATTAGTAT+AGG + chr7_4:52426970-52426989 None:intergenic 20.0%
!! AGTAGATAATTATAATCTTG+AGG + chr7_4:52427136-52427155 None:intergenic 20.0%
!! AGAAATAGTTAATTAATCAC+TGG + chr7_4:52427230-52427249 None:intergenic 20.0%
!! AAATTTAATTGTCAGATATC+TGG - chr7_4:52427310-52427329 Msa1162040:intron 20.0%
!! CTAAATTGTTGAGAAAATTT+AGG + chr7_4:52427663-52427682 None:intergenic 20.0%
!!! TCATAATCAAGGATTTTAAT+TGG - chr7_4:52427777-52427796 Msa1162040:CDS 20.0%
!!! CTAACTAACTTTATTTTTGT+AGG - chr7_4:52427821-52427840 Msa1162040:CDS 20.0%
!! TGAAAAAAATTTACAAGATG+AGG - chr7_4:52428081-52428100 Msa1162040:intron 20.0%
!!! ATTTTTCTGTAATTTGTTTC+AGG - chr7_4:52428175-52428194 Msa1162040:intron 20.0%
!! TGATTTATATATCTCAGTAT+TGG - chr7_4:52428373-52428392 Msa1162040:intron 20.0%
!!! TCCTTAAAGTTTAAAATTAC+CGG + chr7_4:52428499-52428518 None:intergenic 20.0%
!!! TTTTAAAATAAGTGCATGTT+TGG - chr7_4:52428607-52428626 Msa1162040:intron 20.0%
!!! ATGTAGTTTTAACAAAATCA+CGG - chr7_4:52428839-52428858 Msa1162040:CDS 20.0%
!! TCTCATAAATTGACAATAAA+TGG + chr7_4:52428986-52429005 None:intergenic 20.0%
!! ATAAATGATAGTTTAATCAC+TGG - chr7_4:52429015-52429034 Msa1162040:intron 20.0%
!! TAAATGATAGTTTAATCACT+GGG - chr7_4:52429016-52429035 Msa1162040:intron 20.0%
!! AATAAGGCATAAATTATCTT+TGG - chr7_4:52429320-52429339 Msa1162040:intron 20.0%
!! AAAAAAGCCATGTAAAAAAA+TGG - chr7_4:52429462-52429481 Msa1162040:CDS 20.0%
!! TCACAGAAATATACAAATTA+AGG - chr7_4:52429816-52429835 Msa1162040:CDS 20.0%
!!! AAACTAATTGCATTTTTGTA+TGG + chr7_4:52429872-52429891 None:intergenic 20.0%
!! CTACAAATTAATGTTCAATT+GGG + chr7_4:52429933-52429952 None:intergenic 20.0%
!! ACTACAAATTAATGTTCAAT+TGG + chr7_4:52429934-52429953 None:intergenic 20.0%
! AGATTGTGTATTTATAGACA+AGG + chr7_4:52426342-52426361 None:intergenic 25.0%
! ATATATAGATGCACTTTGAA+AGG + chr7_4:52426442-52426461 None:intergenic 25.0%
! TTTGTATGCCTATACTAATT+TGG - chr7_4:52426959-52426978 Msa1162040:intron 25.0%
! AAAAATGTGGAGCTTAAAAA+CGG + chr7_4:52427254-52427273 None:intergenic 25.0%
! ACAATTATGATTTGACTGTT+CGG - chr7_4:52427736-52427755 Msa1162040:CDS 25.0%
! TTGATTATGAAATCTGTAAC+AGG + chr7_4:52427767-52427786 None:intergenic 25.0%
! TGTTACAGATTTCATAATCA+AGG - chr7_4:52427766-52427785 Msa1162040:CDS 25.0%
! GATTGTATATCAATTGTAGA+TGG - chr7_4:52428202-52428221 Msa1162040:CDS 25.0%
! GATAGATTAGTATATCAAAG+TGG + chr7_4:52428528-52428547 None:intergenic 25.0%
! TTGTCAATCATCATATTTAG+TGG + chr7_4:52428553-52428572 None:intergenic 25.0%
!! TTTACGTTAAACAAAAGCAT+TGG + chr7_4:52428691-52428710 None:intergenic 25.0%
!! AAATTGAAGCTTCATGTTTT+CGG - chr7_4:52428724-52428743 Msa1162040:intron 25.0%
! TATTTACTTTGCTTCACTTA+CGG + chr7_4:52429105-52429124 None:intergenic 25.0%
! ATCAAACACAATAATAGTGT+TGG - chr7_4:52429154-52429173 Msa1162040:intron 25.0%
! AAACCAATGTATAGGAAAAA+GGG + chr7_4:52429289-52429308 None:intergenic 25.0%
! TAAACCAATGTATAGGAAAA+AGG + chr7_4:52429290-52429309 None:intergenic 25.0%
!!! ATAAATTATCTTTGGTCTTG+CGG - chr7_4:52429328-52429347 Msa1162040:intron 25.0%
! CAAATTTCAGAACATTGAAA+TGG - chr7_4:52429390-52429409 Msa1162040:intron 25.0%
! ATACTCTTAATATGTGAAGA+AGG - chr7_4:52429723-52429742 Msa1162040:CDS 25.0%
! ACATGTTTCATATTGTTGTA+AGG - chr7_4:52430010-52430029 Msa1162040:five_prime_UTR 25.0%
ATAGGTATGGAAGTTTAGTT+AGG + chr7_4:52426260-52426279 None:intergenic 30.0%
TCAACGTATGTAGTAATAAG+CGG + chr7_4:52426583-52426602 None:intergenic 30.0%
TAATGTATCACCATCATCAT+AGG + chr7_4:52426617-52426636 None:intergenic 30.0%
TCAAAGATTATGGAGCTAAA+GGG - chr7_4:52426725-52426744 Msa1162040:intron 30.0%
TGAATATTAAATGTCCCACA+TGG + chr7_4:52427055-52427074 None:intergenic 30.0%
ATGTGGGACATTTAATATTC+AGG - chr7_4:52427054-52427073 Msa1162040:intron 30.0%
TAATATTCAGGCATTCAAGA+AGG - chr7_4:52427066-52427085 Msa1162040:intron 30.0%
ACTGATATGTTAGATTTGCA+AGG + chr7_4:52427181-52427200 None:intergenic 30.0%
CAAATCTAACATATCAGTTC+AGG - chr7_4:52427183-52427202 Msa1162040:intron 30.0%
GTTCAGCAACTATTAGTTAA+TGG - chr7_4:52427403-52427422 Msa1162040:intron 30.0%
! GAGAAAAGGCATAAAAAAGT+TGG + chr7_4:52427589-52427608 None:intergenic 30.0%
ACGATTGAGATTTGACTAAA+CGG + chr7_4:52427687-52427706 None:intergenic 30.0%
! TCTAACATTTGATAGTTGCA+AGG - chr7_4:52427845-52427864 Msa1162040:intron 30.0%
CTTTAACATTAATCGAATCC+TGG + chr7_4:52427926-52427945 None:intergenic 30.0%
TCGATTAATGTTAAAGCTTC+TGG - chr7_4:52427930-52427949 Msa1162040:intron 30.0%
! ATTGGTGATATGAATTCCAT+CGG + chr7_4:52427991-52428010 None:intergenic 30.0%
!! GATTTTGATGTTTTGGGTAT+TGG + chr7_4:52428009-52428028 None:intergenic 30.0%
!! GCTAGAGATTTTGATGTTTT+GGG + chr7_4:52428015-52428034 None:intergenic 30.0%
!! AGCTAGAGATTTTGATGTTT+TGG + chr7_4:52428016-52428035 None:intergenic 30.0%
!! AAAATCTCTAGCTCTATCAT+AGG - chr7_4:52428023-52428042 Msa1162040:intron 30.0%
!! AAGATTTCTGGAACTACAAA+TGG - chr7_4:52428454-52428473 Msa1162040:intron 30.0%
ATGGAGTTAGAATTAAGACT+TGG - chr7_4:52428473-52428492 Msa1162040:intron 30.0%
! GCCGGTAATTTTAAACTTTA+AGG - chr7_4:52428495-52428514 Msa1162040:intron 30.0%
AGATTAGTATATCAAAGTGG+TGG + chr7_4:52428525-52428544 None:intergenic 30.0%
TAAGTGCATGTTTGGTATAA+TGG - chr7_4:52428615-52428634 Msa1162040:intron 30.0%
GAAAACATGAAGCTTCAATT+TGG + chr7_4:52428725-52428744 None:intergenic 30.0%
!!! TAGTTTTAACAAAATCACGG+TGG - chr7_4:52428842-52428861 Msa1162040:CDS 30.0%
TTGGAAACTCAATGATTGTT+TGG + chr7_4:52428881-52428900 None:intergenic 30.0%
TAAATATTTCTCGTGTCCAT+TGG - chr7_4:52429066-52429085 Msa1162040:CDS 30.0%
AACCAATGTATAGGAAAAAG+GGG + chr7_4:52429288-52429307 None:intergenic 30.0%
CTGATTAATTATTATGGGGT+TGG + chr7_4:52429425-52429444 None:intergenic 30.0%
!!! TGATTTTGTGTTGTTTATGC+AGG - chr7_4:52429578-52429597 Msa1162040:CDS 30.0%
AATGTTCAATTGTCTTACAC+AGG - chr7_4:52429771-52429790 Msa1162040:CDS 30.0%
ATGAAACATGTCACAAGTAA+TGG + chr7_4:52430001-52430020 None:intergenic 30.0%
AAAAGCTATGTATCGAGGTA+TGG + chr7_4:52426380-52426399 None:intergenic 35.0%
TTAGCAAAAGCTATGTATCG+AGG + chr7_4:52426385-52426404 None:intergenic 35.0%
CAACGTATGTAGTAATAAGC+GGG + chr7_4:52426582-52426601 None:intergenic 35.0%
GTCAAAGATTATGGAGCTAA+AGG - chr7_4:52426724-52426743 Msa1162040:intron 35.0%
CAAAGATTATGGAGCTAAAG+GGG - chr7_4:52426726-52426745 Msa1162040:intron 35.0%
! TTTTCCTATAATTGGCCATG+TGG - chr7_4:52427037-52427056 Msa1162040:intron 35.0%
TTTCCTATAATTGGCCATGT+GGG - chr7_4:52427038-52427057 Msa1162040:intron 35.0%
TGATCTTCACTGTAATCTGA+TGG + chr7_4:52427358-52427377 None:intergenic 35.0%
ACAGTGAAGATCAAAGACAT+TGG - chr7_4:52427365-52427384 Msa1162040:intron 35.0%
CAGCAACTATTAGTTAATGG+TGG - chr7_4:52427406-52427425 Msa1162040:intron 35.0%
CAACTATTAGTTAATGGTGG+TGG - chr7_4:52427409-52427428 Msa1162040:intron 35.0%
! TATGTTTCCTTTTCCATCGA+TGG + chr7_4:52427437-52427456 None:intergenic 35.0%
TCGATGGAAAAGGAAACATA+TGG - chr7_4:52427437-52427456 Msa1162040:intron 35.0%
ATGGAAAAGGAAACATATGG+TGG - chr7_4:52427440-52427459 Msa1162040:intron 35.0%
GATCTATGTATACATACCGT+TGG + chr7_4:52427638-52427657 None:intergenic 35.0%
ACAAATTCACGTTTCATTCC+AGG - chr7_4:52427905-52427924 Msa1162040:intron 35.0%
! ATATGAATTCCATCGGTGTT+AGG + chr7_4:52427984-52428003 None:intergenic 35.0%
! ATATCTCAGTATTGGAAGCT+TGG - chr7_4:52428381-52428400 Msa1162040:intron 35.0%
! TATCTCAGTATTGGAAGCTT+GGG - chr7_4:52428382-52428401 Msa1162040:intron 35.0%
AAAATCCAAGGACATCGTTT+CGG - chr7_4:52428411-52428430 Msa1162040:intron 35.0%
AGTTAGAATTAAGACTTGGC+CGG - chr7_4:52428477-52428496 Msa1162040:intron 35.0%
TTAACAAAATCACGGTGGTT+TGG - chr7_4:52428847-52428866 Msa1162040:CDS 35.0%
TGATAAGTCTAGTGCATGTT+TGG + chr7_4:52428920-52428939 None:intergenic 35.0%
! ATTTATTTAGTGGATCACGC+TGG + chr7_4:52429047-52429066 None:intergenic 35.0%
! AATAGTGTTGGACCAATAGA+TGG - chr7_4:52429166-52429185 Msa1162040:intron 35.0%
TGTATGTTTCCTCCATCTAT+TGG + chr7_4:52429181-52429200 None:intergenic 35.0%
ACACTACAAGTACACTATAC+TGG - chr7_4:52429514-52429533 Msa1162040:CDS 35.0%
GGTACAACACATTGCTAATT+TGG + chr7_4:52429614-52429633 None:intergenic 35.0%
GTGTTGTACCAGAATATAAC+AGG - chr7_4:52429624-52429643 Msa1162040:CDS 35.0%
! CACTTTTGCCTGTTATATTC+TGG + chr7_4:52429635-52429654 None:intergenic 35.0%
! TGCATTTTTATCTCCTTGCA+TGG + chr7_4:52429699-52429718 None:intergenic 35.0%
AGAGTGTGGTATAGAGACTT+TGG - chr7_4:52426211-52426230 Msa1162040:three_prime_UTR 40.0%
GAGTGTGGTATAGAGACTTT+GGG - chr7_4:52426212-52426231 Msa1162040:three_prime_UTR 40.0%
TGTATCGAGGTATGGAACTA+AGG + chr7_4:52426372-52426391 None:intergenic 40.0%
! TAGATGCACTTTGAAAGGTG+AGG + chr7_4:52426437-52426456 None:intergenic 40.0%
ATGAGAGGAGGAATTGTCTT+AGG + chr7_4:52426507-52426526 None:intergenic 40.0%
! TGATGACGATAGTGAATGAG+AGG + chr7_4:52426522-52426541 None:intergenic 40.0%
TTGCAATAGCAACTTGCAAG+AGG - chr7_4:52426555-52426574 Msa1162040:CDS 40.0%
GATTCACCTTCCTATGATGA+TGG - chr7_4:52426604-52426623 Msa1162040:CDS 40.0%
GTATCACCATCATCATAGGA+AGG + chr7_4:52426613-52426632 None:intergenic 40.0%
! GATTTTTCGTGCTTAGACCA+TGG - chr7_4:52426670-52426689 Msa1162040:intron 40.0%
TGGGAAGTAGTATGTGATTC+AGG - chr7_4:52427091-52427110 Msa1162040:intron 40.0%
GGGAAGTAGTATGTGATTCA+GGG - chr7_4:52427092-52427111 Msa1162040:intron 40.0%
CTCAAACCAATCAGATTCTC+TGG - chr7_4:52427154-52427173 Msa1162040:intron 40.0%
AGGCTGCAAAAAGAGAGAAA+AGG + chr7_4:52427603-52427622 None:intergenic 40.0%
!! TCTAGCTCTATCATAGGAAC+TGG - chr7_4:52428029-52428048 Msa1162040:intron 40.0%
AAGTTAACTACCTGATGCCA+TGG + chr7_4:52428270-52428289 None:intergenic 40.0%
! ATCTCAGTATTGGAAGCTTG+GGG - chr7_4:52428383-52428402 Msa1162040:intron 40.0%
AAATCCAAGGACATCGTTTC+GGG - chr7_4:52428412-52428431 Msa1162040:intron 40.0%
!! GTTTCGGGAGTATTAGTGAA+TGG - chr7_4:52428427-52428446 Msa1162040:intron 40.0%
GTGAATGGAGCTAAGATTTC+TGG - chr7_4:52428442-52428461 Msa1162040:intron 40.0%
TTTCGGACGTTTGATGCATT+TGG - chr7_4:52428741-52428760 Msa1162040:CDS 40.0%
CAACAAACTCAGTGACGATT+TGG + chr7_4:52428775-52428794 None:intergenic 40.0%
AGGAAAAAGGGGAGTAAGTT+AGG + chr7_4:52429277-52429296 None:intergenic 40.0%
! CTCCCCTTTTTCCTATACAT+TGG - chr7_4:52429283-52429302 Msa1162040:intron 40.0%
TCTTGCGGTGATTAAATGCA+GGG - chr7_4:52429343-52429362 Msa1162040:intron 40.0%
CAGTGAGAAATTTCCATGCA+AGG - chr7_4:52429683-52429702 Msa1162040:CDS 40.0%
TTGTTGCATAAAGCCTTAGC+AGG + chr7_4:52429753-52429772 None:intergenic 40.0%
CAAACGTTGTATGGAGCAAC+AGG - chr7_4:52426187-52426206 Msa1162040:three_prime_UTR 45.0%
TCGAGGTATGGAACTAAGGA+GGG + chr7_4:52426368-52426387 None:intergenic 45.0%
ATCGAGGTATGGAACTAAGG+AGG + chr7_4:52426369-52426388 None:intergenic 45.0%
AGAGGAGGAATTGTCTTAGG+AGG + chr7_4:52426504-52426523 None:intergenic 45.0%
TGACGATAGTGAATGAGAGG+AGG + chr7_4:52426519-52426538 None:intergenic 45.0%
GATTATGGAGCTAAAGGGGA+TGG - chr7_4:52426730-52426749 Msa1162040:intron 45.0%
TGGTCATACTGATGACACAC+AGG - chr7_4:52426750-52426769 Msa1162040:CDS 45.0%
TGTCCCACATGGCCAATTAT+AGG + chr7_4:52427044-52427063 None:intergenic 45.0%
TTCAGGCATTCAAGAAGGCT+TGG - chr7_4:52427071-52427090 Msa1162040:intron 45.0%
TCAGGCATTCAAGAAGGCTT+GGG - chr7_4:52427072-52427091 Msa1162040:intron 45.0%
!! TTCAGGGCAAGCAGTTTTAG+TGG - chr7_4:52427108-52427127 Msa1162040:intron 45.0%
CAAGGACCAGAGAATCTGAT+TGG + chr7_4:52427163-52427182 None:intergenic 45.0%
GGTGGCAAAACTCATGCAAA+AGG - chr7_4:52427458-52427477 Msa1162040:intron 45.0%
! TCTCTTTTTGCAGCCTTGCA+AGG - chr7_4:52427607-52427626 Msa1162040:intron 45.0%
GAAGCCACAGACATTACTTG+TGG - chr7_4:52428238-52428257 Msa1162040:CDS 45.0%
TGGTCCACAAGTAATGTCTG+TGG + chr7_4:52428245-52428264 None:intergenic 45.0%
ACAGACATTACTTGTGGACC+AGG - chr7_4:52428244-52428263 Msa1162040:CDS 45.0%
! TCTCAGTATTGGAAGCTTGG+GGG - chr7_4:52428384-52428403 Msa1162040:intron 45.0%
! TCAGTGACGATTTGGATCTG+CGG + chr7_4:52428767-52428786 None:intergenic 45.0%
!! TGGCATCTTGGTGGATTTGT+TGG + chr7_4:52428900-52428919 None:intergenic 45.0%
CTAGTGCATGTTTGGCATCT+TGG + chr7_4:52428912-52428931 None:intergenic 45.0%
! TATTTAGTGGATCACGCTGG+AGG + chr7_4:52429044-52429063 None:intergenic 45.0%
CGGTCAATTCATGAGACCAA+TGG + chr7_4:52429085-52429104 None:intergenic 45.0%
! AGTGTTGGACCAATAGATGG+AGG - chr7_4:52429169-52429188 Msa1162040:intron 45.0%
GTCTTGCGGTGATTAAATGC+AGG - chr7_4:52429342-52429361 Msa1162040:intron 45.0%
GATTAAATGCAGGGAGGTTC+AGG - chr7_4:52429352-52429371 Msa1162040:intron 45.0%
GTTGTTTATGCAGGACTCTG+CGG - chr7_4:52429587-52429606 Msa1162040:CDS 45.0%
TGAAGAAGGAGAACCTGCTA+AGG - chr7_4:52429737-52429756 Msa1162040:CDS 45.0%
ATTTCTGTGAGTTGCAGCGT+GGG + chr7_4:52429806-52429825 None:intergenic 45.0%
! TATTTCTGTGAGTTGCAGCG+TGG + chr7_4:52429807-52429826 None:intergenic 45.0%
AGGTATGGAACTAAGGAGGG+GGG + chr7_4:52426365-52426384 None:intergenic 50.0%
GAGGTATGGAACTAAGGAGG+GGG + chr7_4:52426366-52426385 None:intergenic 50.0%
CGAGGTATGGAACTAAGGAG+GGG + chr7_4:52426367-52426386 None:intergenic 50.0%
GAGAACGTGAAGTGACACCA+TGG + chr7_4:52426690-52426709 None:intergenic 50.0%
!! AATGGTGGTGGAACCATCGA+TGG - chr7_4:52427421-52427440 Msa1162040:intron 50.0%
GGTGGAACCATCGATGGAAA+AGG - chr7_4:52427427-52427446 Msa1162040:intron 50.0%
GTCTCAATGTGTCATCGCCA+GGG - chr7_4:52427952-52427971 Msa1162040:intron 50.0%
!! TCGGTGTTAGGACTATTCCC+TGG + chr7_4:52427972-52427991 None:intergenic 50.0%
GGGAATAGTCCTAACACCGA+TGG - chr7_4:52427972-52427991 Msa1162040:CDS 50.0%
ATTACTTGTGGACCAGGCCA+TGG - chr7_4:52428250-52428269 Msa1162040:CDS 50.0%
TACTCCCGAAACGATGTCCT+TGG + chr7_4:52428419-52428438 None:intergenic 50.0%
GTGCATGTTTGGCATCTTGG+TGG + chr7_4:52428909-52428928 None:intergenic 50.0%
TGCGGTGATTAAATGCAGGG+AGG - chr7_4:52429346-52429365 Msa1162040:intron 50.0%
ATGGAGCAACAGGCAGAGTG+TGG - chr7_4:52426197-52426216 Msa1162040:three_prime_UTR 55.0%
GCCTTGCAAGGATGCTCCAA+CGG - chr7_4:52427619-52427638 Msa1162040:intron 55.0%
! ACCGTTGGAGCATCCTTGCA+AGG + chr7_4:52427623-52427642 None:intergenic 55.0%
GGTCTCAATGTGTCATCGCC+AGG - chr7_4:52427951-52427970 Msa1162040:intron 55.0%
AACTACCTGATGCCATGGCC+TGG + chr7_4:52428265-52428284 None:intergenic 55.0%
! AGTATTGGAAGCTTGGGGGC+TGG - chr7_4:52428388-52428407 Msa1162040:intron 55.0%
CTTGGGGGCTGGAAAATCCA+AGG - chr7_4:52428399-52428418 Msa1162040:intron 55.0%
GTGGACCAGGCCATGGCATC+AGG - chr7_4:52428257-52428276 Msa1162040:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 52426186 52430047 52426186 ID=Msa1162040;Name=Msa1162040
chr7_4 mRNA 52426186 52430047 52426186 ID=Msa1162040-mRNA-1;Parent=Msa1162040;Name=Msa1162040-mRNA-1;_AED=0.30;_eAED=0.30;_QI=124|1|1|1|1|1|9|211|511
chr7_4 exon 52426186 52426633 52426186 ID=Msa1162040-mRNA-1:exon:15097;Parent=Msa1162040-mRNA-1
chr7_4 exon 52426750 52426869 52426750 ID=Msa1162040-mRNA-1:exon:15096;Parent=Msa1162040-mRNA-1
chr7_4 exon 52427735 52427843 52427735 ID=Msa1162040-mRNA-1:exon:15095;Parent=Msa1162040-mRNA-1
chr7_4 exon 52427955 52428036 52427955 ID=Msa1162040-mRNA-1:exon:15094;Parent=Msa1162040-mRNA-1
chr7_4 exon 52428183 52428390 52428183 ID=Msa1162040-mRNA-1:exon:15093;Parent=Msa1162040-mRNA-1
chr7_4 exon 52428593 52428613 52428593 ID=Msa1162040-mRNA-1:exon:15092;Parent=Msa1162040-mRNA-1
chr7_4 exon 52428741 52428908 52428741 ID=Msa1162040-mRNA-1:exon:15091;Parent=Msa1162040-mRNA-1
chr7_4 exon 52429029 52429157 52429029 ID=Msa1162040-mRNA-1:exon:15090;Parent=Msa1162040-mRNA-1
chr7_4 exon 52429462 52430047 52429462 ID=Msa1162040-mRNA-1:exon:15089;Parent=Msa1162040-mRNA-1
chr7_4 five_prime_UTR 52429924 52430047 52429924 ID=Msa1162040-mRNA-1:five_prime_utr;Parent=Msa1162040-mRNA-1
chr7_4 CDS 52429462 52429923 52429462 ID=Msa1162040-mRNA-1:cds;Parent=Msa1162040-mRNA-1
chr7_4 CDS 52429029 52429157 52429029 ID=Msa1162040-mRNA-1:cds;Parent=Msa1162040-mRNA-1
chr7_4 CDS 52428741 52428908 52428741 ID=Msa1162040-mRNA-1:cds;Parent=Msa1162040-mRNA-1
chr7_4 CDS 52428593 52428613 52428593 ID=Msa1162040-mRNA-1:cds;Parent=Msa1162040-mRNA-1
chr7_4 CDS 52428183 52428390 52428183 ID=Msa1162040-mRNA-1:cds;Parent=Msa1162040-mRNA-1
chr7_4 CDS 52427955 52428036 52427955 ID=Msa1162040-mRNA-1:cds;Parent=Msa1162040-mRNA-1
chr7_4 CDS 52427735 52427843 52427735 ID=Msa1162040-mRNA-1:cds;Parent=Msa1162040-mRNA-1
chr7_4 CDS 52426750 52426869 52426750 ID=Msa1162040-mRNA-1:cds;Parent=Msa1162040-mRNA-1
chr7_4 CDS 52426397 52426633 52426397 ID=Msa1162040-mRNA-1:cds;Parent=Msa1162040-mRNA-1
chr7_4 three_prime_UTR 52426186 52426396 52426186 ID=Msa1162040-mRNA-1:three_prime_utr;Parent=Msa1162040-mRNA-1
Gene Sequence

>Msa1162040

ATGAAAAGCAAACATGATCTTATTAATTTCCTTGTCTATAAATACACAATCTCCCCCCTCCTTAGTTCCATACCTCGATACATAGCTTTTGCTAAATATATTCTCATCAAACAATTATATTATTCCTCACCTTTCAAAGTGCATCTATATATTTTTCTCAATATAGTAAAATACAAAAGAACTATAATGGTCCTCCTAAGACAATTCCTCCTCTCATTCACTATCGTCATCATTTCATTTGTTGCTTGCAATAGCAACTTGCAAGAGGACCCGCTTATTACTACATACGTTGATGATTCACCTTCCTATGATGATGGTGATACATTATTATTCAAGAACTTGGTTGAGCAAAACACAAATGATTTTTCGTGCTTAGACCATGGTGTCACTTCACGTTCTCTTAAAATTGTTAATGTCAAAGATTATGGAGCTAAAGGGGATGGTCATACTGATGACACACAGGCATTCAAGAAGGCTTGGGAAGTAGTATGTGATTCAGGGCAAGCAGTTTTAGTGGCACCTCAAGATTATAATTATCTACTCAAACCAATCAGATTCTCTGGTCCTTGCAAATCTAACATATCAGTTCAGATATCTGGAAGTCTTGAAGCATCAGATAATCCATCAGATTACAGTGAAGATCAAAGACATTGGCTTGTATTTGAGCAAGTTCAGCAACTATTAGTTAATGGTGGTGGAACCATCGATGGAAAAGGAAACATATGGTGGCAAAACTCATGCAAAAGGAACAAAAAGAAACCTTGCAAGGATGCTCCAACGGCTCTAACATTTGATAGTTGCAAGGACTTAATAGTTGACGATTTGACAATACAAAATGCGCAACAAATTCACGTTTCATTCCAGGATTCGATTAATGTTAAAGCTTCTGGTCTCAATGTGTCATCGCCAGGGAATAGTCCTAACACCGATGGAATTCATATCACCAATACCCAAAACATCAAAATCTCTAGCTCTATCATAGGAACTGGTGACGATTGTATATCAATTGTAGATGGATCTAGTAATGTAGAAGCCACAGACATTACTTGTGGACCAGGCCATGGCATCAGTATTGGAAGCTTGGGGGCTGGAAAATCCAAGGACATCGTTTCGGGAGTATTAGTGAATGGAGCTAAGATTTCTGGAACTACAAATGGAGTTAGAATTAAGACTTGGCCGGGAGGTTCAGGAAGTGCTAGCAACATCAAATTTCAGAACATTGAAATGGACAATGTGACCAACCCCATAATAATTAATCAGAATTACTGTGACAAGAAAAAAAAGCCATGTAAAAAAATGGACTCTGCGGTCCAAATTAGCAATGTGTTGTACCAGAATATAACAGGCAAAAGTGCTTCTGATATAGCTGTAAAGTTTGATTGCAGTGAGAAATTTCCATGCAAGGAGATAAAAATGCAAAACATACTCTTAATATGTGAAGAAGGAGAACCTGCTAAGGCTTTATGCAACAATGTTCAATTGTCTTACACAGGAAATGTTAACCCACGCTGCAACTCACAGAAATATACAAATTAA
Protein sequence

>Msa1162040

MKSKHDLINFLVYKYTISPLLSSIPRYIAFAKYILIKQLYYSSPFKVHLYIFLNIVKYKRTIMVLLRQFLLSFTIVIISFVACNSNLQEDPLITTYVDDSPSYDDGDTLLFKNLVEQNTNDFSCLDHGVTSRSLKIVNVKDYGAKGDGHTDDTQAFKKAWEVVCDSGQAVLVAPQDYNYLLKPIRFSGPCKSNISVQISGSLEASDNPSDYSEDQRHWLVFEQVQQLLVNGGGTIDGKGNIWWQNSCKRNKKKPCKDAPTALTFDSCKDLIVDDLTIQNAQQIHVSFQDSINVKASGLNVSSPGNSPNTDGIHITNTQNIKISSSIIGTGDDCISIVDGSSNVEATDITCGPGHGISIGSLGAGKSKDIVSGVLVNGAKISGTTNGVRIKTWPGGSGSASNIKFQNIEMDNVTNPIIINQNYCDKKKKPCKKMDSAVQISNVLYQNITGKSASDIAVKFDCSEKFPCKEIKMQNILLICEEGEPAKALCNNVQLSYTGNVNPRCNSQKYTN*