AlfalfaGEDB | Msa1168520

M. sativa cultivar zhongmu-4 / Msa1168520

KEGG: K10775|K13064
GO: GO:0001101 GO:0003674 GO:0003824 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009636 GO:0009698 GO:0009699 GO:0009719 GO:0009725 GO:0009739 GO:0009987 GO:0010033 GO:0010243 GO:0014070 GO:0016598 GO:0016829 GO:0016840 GO:0016841 GO:0019438 GO:0019538 GO:0019748 GO:0019941 GO:0030163 GO:0033993 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044550 GO:0045548 GO:0046677 GO:0046898 GO:0050896 GO:0051603 GO:0060992 GO:0071704 GO:0097305 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901654 GO:1901698 GO:1901700
PFAM: PF00221.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1168520	XP_039683519.1	97.203	143	4	0	1	143	1	143	3.46e-92	292

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1168520	sp\|P27990\|PALY_MEDSA	94.444	144	7	1	1	143	1	144	1.30e-90	280

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1168520	A0A396H7I3	97.203	143	4	0	1	143	1	143	1.65e-92	292

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1078560	Msa1168520	0.808109	3.639996e-50	-8.615850e-47
Msa1079360	Msa1168520	0.836884	7.203799e-57	-8.615850e-47
Msa1079390	Msa1168520	0.860986	1.408564e-63	-8.615850e-47
Msa1079400	Msa1168520	0.870079	1.912627e-66	-8.615850e-47
Msa1123590	Msa1168520	0.833235	5.991625e-56	-8.615850e-47
Msa1123600	Msa1168520	0.923398	2.797881e-89	-8.615850e-47
Msa1123610	Msa1168520	0.815185	1.052528e-51	-8.615850e-47
Msa1168520	Msa1168540	0.815753	7.867491e-52	-8.615850e-47
Msa1168520	Msa1168620	0.863617	2.191182e-64	-8.615850e-47
Msa1168520	Msa1168700	0.870113	1.864257e-66	-8.615850e-47
Msa1168520	Msa1168710	0.899058	2.884228e-77	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1168520	MtrunA17_Chr7g0264341	97.203	143	4	0	1	143	1	143	3.18e-96	292
Msa1168520	MtrunA17_Chr7g0264351	97.203	143	4	0	1	143	1	143	3.43e-96	292
Msa1168520	MtrunA17_Chr7g0264311	97.203	143	4	0	1	143	1	143	3.78e-96	292
Msa1168520	MtrunA17_Chr1g0181091	75.000	136	26	1	8	143	4	131	1.80e-66	214
Msa1168520	MtrunA17_Chr1g0199551	78.070	114	25	0	30	143	28	141	1.49e-58	192
Msa1168520	MtrunA17_Chr5g0448361	67.544	114	36	1	31	143	16	129	2.34e-47	162
Msa1168520	MtrunA17_Chr2g0326921	65.812	117	38	1	27	143	1	115	2.15e-45	157

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1168520	AT2G37040.1	77.165	127	29	0	17	143	18	144	6.80e-62	202
Msa1168520	AT3G53260.1	81.250	112	21	0	32	143	25	136	9.71e-59	193
Msa1168520	AT3G10340.1	75.207	121	30	0	23	143	6	126	2.03e-57	190
Msa1168520	AT5G04230.1	74.107	112	27	1	32	143	12	121	2.27e-51	173
Msa1168520	AT5G04230.2	74.107	112	27	1	32	143	12	121	2.81e-51	173

Find 51 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGCAAAGCACCACCTTGTT+TGG	0.301759	7_4:-61166901	Msa1168510:intron
GAGTTAAGGCCAGCAGTGAC+TGG	0.386890	7_4:+61166802	Msa1168520:CDS
ATGAAGCTGATCCTTTGAAT+TGG	0.399923	7_4:+61166601	Msa1168520:CDS
TACGGTGTCACCACCGGGTT+CGG	0.400782	7_4:+61166858	Msa1168520:CDS
ATCTGCTAGGGCTGGAGTTA+AGG	0.404467	7_4:+61166788	Msa1168520:CDS
TGAAGCTGATCCTTTGAATT+GGG	0.415897	7_4:+61166602	Msa1168520:CDS
AATCAAGCAGAATGATTCAT+TGG	0.416820	7_4:-61166550	Msa1168510:three_prime_UTR
CAAGACGGACCACCGGCTTC+CGG	0.424699	7_4:-61166689	Msa1168510:intron
CGGAAGCCGGTGGTCCGTCT+TGG	0.445172	7_4:+61166690	Msa1168520:CDS
GTGCGAGGTGGCGCCGAACC+CGG	0.448163	7_4:-61166871	Msa1168510:intron
CTCCATCACCCAGTCACTGC+TGG	0.457530	7_4:-61166811	Msa1168510:intron
CTGTCTGAATCTGCTAGGGC+TGG	0.469696	7_4:+61166780	Msa1168520:CDS
AAGCCGGTGGTCCGTCTTGG+TGG	0.472146	7_4:+61166693	Msa1168520:CDS
TGGAGCTGTCTGAATCTGCT+AGG	0.480887	7_4:+61166775	Msa1168520:CDS
GACAGTTACGGTGTCACCAC+CGG	0.492715	7_4:+61166852	Msa1168520:CDS
CGAGACACTGACGATTTCTC+AGG	0.498607	7_4:+61166716	Msa1168520:CDS
TGCACATGACCATGGTGTGC+AGG	0.506601	7_4:+61166752	Msa1168520:CDS
CTTTGCAGAAAGAACTCATA+AGG	0.510191	7_4:+61166916	Msa1168520:CDS
AGCGTATGGTGGAGGAGTAC+CGG	0.511195	7_4:+61166670	Msa1168520:CDS
AACAAAGGCACGGACAGTTA+CGG	0.520670	7_4:+61166840	Msa1168520:CDS
GGCCAGCAGTGACTGGGTGA+TGG	0.539190	7_4:+61166809	Msa1168520:CDS
ACCATGGTCATGTGCAGCAA+TGG	0.541335	7_4:-61166745	Msa1168510:intron
AAGCACCACCTTGTTTGGTA+CGG	0.564097	7_4:-61166896	Msa1168510:intron
GGTGGAGGAGTACCGGAAGC+CGG	0.566790	7_4:+61166677	Msa1168520:CDS
GGAGCTGTCTGAATCTGCTA+GGG	0.568528	7_4:+61166776	Msa1168520:CDS
TTTGGTACGGCGGTGCGAGG+TGG	0.578567	7_4:-61166883	Msa1168510:intron
GCTGCCACACCCCAATTCAA+AGG	0.581598	7_4:-61166612	Msa1168510:intron
TCGCCACCAAGACGGACCAC+CGG	0.582161	7_4:-61166696	Msa1168510:intron
TCGCACCGCCGTACCAAACA+AGG	0.582783	7_4:+61166888	Msa1168520:CDS
TGAGGTGAAGCGTATGGTGG+AGG	0.584737	7_4:+61166662	Msa1168520:CDS
GCCATTGCTGCACATGACCA+TGG	0.585263	7_4:+61166744	Msa1168520:CDS
TGATCCTTTGAATTGGGGTG+TGG	0.587095	7_4:+61166608	Msa1168520:CDS
AGTTAAGGCCAGCAGTGACT+GGG	0.592895	7_4:+61166803	Msa1168520:CDS
GTGGCAGCTGAGGCAATGAA+AGG	0.593497	7_4:+61166627	Msa1168520:CDS
CACCACCTTGTTTGGTACGG+CGG	0.598168	7_4:-61166893	Msa1168510:intron
TTGTTTGGTACGGCGGTGCG+AGG	0.600854	7_4:-61166886	Msa1168510:intron
ACAGTTACGGTGTCACCACC+GGG	0.611533	7_4:+61166853	Msa1168520:CDS
GAATTGGGGTGTGGCAGCTG+AGG	0.617765	7_4:+61166617	Msa1168520:CDS
GACACTGACGATTTCTCAGG+TGG	0.626830	7_4:+61166719	Msa1168520:CDS
CCTTGATGAGGTGAAGCGTA+TGG	0.628479	7_4:+61166656	Msa1168520:CDS
GAAGCTGATCCTTTGAATTG+GGG	0.639983	7_4:+61166603	Msa1168520:CDS
GAAAGGCAGTCACCTTGATG+AGG	0.642522	7_4:+61166644	Msa1168520:CDS
GGAGGAGTACCGGAAGCCGG+TGG	0.645003	7_4:+61166680	Msa1168520:CDS
CCATACGCTTCACCTCATCA+AGG	0.653785	7_4:-61166656	Msa1168510:intron
TGATGAGGTGAAGCGTATGG+TGG	0.671300	7_4:+61166659	Msa1168520:CDS
TCAGTGTCTCGCCACCAAGA+CGG	0.673504	7_4:-61166704	Msa1168510:intron
GGAGAGTATGAACAAAGGCA+CGG	0.688439	7_4:+61166830	Msa1168520:CDS
ACAGCTCCACCTGCACACCA+TGG	0.693221	7_4:-61166761	Msa1168510:intron
ACATGACCATGGTGTGCAGG+TGG	0.694429	7_4:+61166755	Msa1168520:CDS
GTGATGGAGAGTATGAACAA+AGG	0.730510	7_4:+61166825	Msa1168520:CDS
CACCGCCGTACCAAACAAGG+TGG	0.735748	7_4:+61166891	Msa1168520:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATCAAGCAGAATGATTCAT+TGG	-	chr7_4:61166553-61166572	Msa1168510:three_prime_UTR	30.0%
	ATGAAGCTGATCCTTTGAAT+TGG	+	chr7_4:61166601-61166620	Msa1168520:CDS	35.0%
	TGAAGCTGATCCTTTGAATT+GGG	+	chr7_4:61166602-61166621	Msa1168520:CDS	35.0%
	CTTTGCAGAAAGAACTCATA+AGG	+	chr7_4:61166916-61166935	Msa1168520:CDS	35.0%
	GAAGCTGATCCTTTGAATTG+GGG	+	chr7_4:61166603-61166622	Msa1168520:CDS	40.0%
!	GTGATGGAGAGTATGAACAA+AGG	+	chr7_4:61166825-61166844	Msa1168520:CDS	40.0%
	TGATCCTTTGAATTGGGGTG+TGG	+	chr7_4:61166608-61166627	Msa1168520:CDS	45.0%
!	GGAGAGTATGAACAAAGGCA+CGG	+	chr7_4:61166830-61166849	Msa1168520:CDS	45.0%
!	AACAAAGGCACGGACAGTTA+CGG	+	chr7_4:61166840-61166859	Msa1168520:CDS	45.0%
!	AAGCACCACCTTGTTTGGTA+CGG	-	chr7_4:61166899-61166918	Msa1168510:intron	45.0%
	GAAAGGCAGTCACCTTGATG+AGG	+	chr7_4:61166644-61166663	Msa1168520:CDS	50.0%
	CCATACGCTTCACCTCATCA+AGG	-	chr7_4:61166659-61166678	Msa1168510:intron	50.0%
	CCTTGATGAGGTGAAGCGTA+TGG	+	chr7_4:61166656-61166675	Msa1168520:CDS	50.0%
	TGATGAGGTGAAGCGTATGG+TGG	+	chr7_4:61166659-61166678	Msa1168520:CDS	50.0%
	CGAGACACTGACGATTTCTC+AGG	+	chr7_4:61166716-61166735	Msa1168520:CDS	50.0%
	GACACTGACGATTTCTCAGG+TGG	+	chr7_4:61166719-61166738	Msa1168520:CDS	50.0%
	ACCATGGTCATGTGCAGCAA+TGG	-	chr7_4:61166748-61166767	Msa1168510:intron	50.0%
	TGGAGCTGTCTGAATCTGCT+AGG	+	chr7_4:61166775-61166794	Msa1168520:CDS	50.0%
	GGAGCTGTCTGAATCTGCTA+GGG	+	chr7_4:61166776-61166795	Msa1168520:CDS	50.0%
	ATCTGCTAGGGCTGGAGTTA+AGG	+	chr7_4:61166788-61166807	Msa1168520:CDS	50.0%
	AGTTAAGGCCAGCAGTGACT+GGG	+	chr7_4:61166803-61166822	Msa1168520:CDS	50.0%
!	CTGCAAAGCACCACCTTGTT+TGG	-	chr7_4:61166904-61166923	Msa1168510:intron	50.0%
	GCTGCCACACCCCAATTCAA+AGG	-	chr7_4:61166615-61166634	Msa1168510:intron	55.0%
	GTGGCAGCTGAGGCAATGAA+AGG	+	chr7_4:61166627-61166646	Msa1168520:CDS	55.0%
	TGAGGTGAAGCGTATGGTGG+AGG	+	chr7_4:61166662-61166681	Msa1168520:CDS	55.0%
!	AGCGTATGGTGGAGGAGTAC+CGG	+	chr7_4:61166670-61166689	Msa1168520:CDS	55.0%
	TCAGTGTCTCGCCACCAAGA+CGG	-	chr7_4:61166707-61166726	Msa1168510:intron	55.0%
	GCCATTGCTGCACATGACCA+TGG	+	chr7_4:61166744-61166763	Msa1168520:CDS	55.0%
	TGCACATGACCATGGTGTGC+AGG	+	chr7_4:61166752-61166771	Msa1168520:CDS	55.0%
	ACATGACCATGGTGTGCAGG+TGG	+	chr7_4:61166755-61166774	Msa1168520:CDS	55.0%
	CTGTCTGAATCTGCTAGGGC+TGG	+	chr7_4:61166780-61166799	Msa1168520:CDS	55.0%
	GAGTTAAGGCCAGCAGTGAC+TGG	+	chr7_4:61166802-61166821	Msa1168520:CDS	55.0%
!!	GACAGTTACGGTGTCACCAC+CGG	+	chr7_4:61166852-61166871	Msa1168520:CDS	55.0%
!!	ACAGTTACGGTGTCACCACC+GGG	+	chr7_4:61166853-61166872	Msa1168520:CDS	55.0%
!	CACCACCTTGTTTGGTACGG+CGG	-	chr7_4:61166896-61166915	Msa1168510:intron	55.0%
	GAATTGGGGTGTGGCAGCTG+AGG	+	chr7_4:61166617-61166636	Msa1168520:CDS	60.0%
	ACAGCTCCACCTGCACACCA+TGG	-	chr7_4:61166764-61166783	Msa1168510:intron	60.0%
	CTCCATCACCCAGTCACTGC+TGG	-	chr7_4:61166814-61166833	Msa1168510:intron	60.0%
!!	TACGGTGTCACCACCGGGTT+CGG	+	chr7_4:61166858-61166877	Msa1168520:CDS	60.0%
	TTGTTTGGTACGGCGGTGCG+AGG	-	chr7_4:61166889-61166908	Msa1168510:intron	60.0%
	TCGCACCGCCGTACCAAACA+AGG	+	chr7_4:61166888-61166907	Msa1168520:CDS	60.0%
	CACCGCCGTACCAAACAAGG+TGG	+	chr7_4:61166891-61166910	Msa1168520:CDS	60.0%
!!	GGTGGAGGAGTACCGGAAGC+CGG	+	chr7_4:61166677-61166696	Msa1168520:CDS	65.0%
!!	CAAGACGGACCACCGGCTTC+CGG	-	chr7_4:61166692-61166711	Msa1168510:intron	65.0%
	AAGCCGGTGGTCCGTCTTGG+TGG	+	chr7_4:61166693-61166712	Msa1168520:CDS	65.0%
	TCGCCACCAAGACGGACCAC+CGG	-	chr7_4:61166699-61166718	Msa1168510:intron	65.0%
	GGCCAGCAGTGACTGGGTGA+TGG	+	chr7_4:61166809-61166828	Msa1168520:CDS	65.0%
	TTTGGTACGGCGGTGCGAGG+TGG	-	chr7_4:61166886-61166905	Msa1168510:intron	65.0%
!!	GGAGGAGTACCGGAAGCCGG+TGG	+	chr7_4:61166680-61166699	Msa1168520:CDS	70.0%
	CGGAAGCCGGTGGTCCGTCT+TGG	+	chr7_4:61166690-61166709	Msa1168520:CDS	70.0%
!	GTGCGAGGTGGCGCCGAACC+CGG	-	chr7_4:61166874-61166893	Msa1168510:intron	75.0%
!!	CGAGGTGGCGCCGAACCCGG+TGG	-	chr7_4:61166871-61166890	Msa1168510:intron	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	61166513	61166950	61166513	ID=Msa1168520;Name=Msa1168520
chr7_4	mRNA	61166513	61166950	61166513	ID=Msa1168520-mRNA-1;Parent=Msa1168520;Name=Msa1168520-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|145
chr7_4	exon	61166513	61166950	61166513	ID=Msa1168520-mRNA-1:exon:19311;Parent=Msa1168520-mRNA-1
chr7_4	CDS	61166513	61166950	61166513	ID=Msa1168520-mRNA-1:cds;Parent=Msa1168520-mRNA-1

Gene Sequence

>Msa1168520

ATGGAAGCAATATCAGCAGCTATCACAAAAAACAATGCCAATGAATCATTCTGCTTGATTCATGCAAAGAATAATAACATGAAAGTGAATGAAGCTGATCCTTTGAATTGGGGTGTGGCAGCTGAGGCAATGAAAGGCAGTCACCTTGATGAGGTGAAGCGTATGGTGGAGGAGTACCGGAAGCCGGTGGTCCGTCTTGGTGGCGAGACACTGACGATTTCTCAGGTGGCTGCCATTGCTGCACATGACCATGGTGTGCAGGTGGAGCTGTCTGAATCTGCTAGGGCTGGAGTTAAGGCCAGCAGTGACTGGGTGATGGAGAGTATGAACAAAGGCACGGACAGTTACGGTGTCACCACCGGGTTCGGCGCCACCTCGCACCGCCGTACCAAACAAGGTGGTGCTTTGCAGAAAGAACTCATAAGGTATTATTCTTAA

Protein sequence

>Msa1168520

MEAISAAITKNNANESFCLIHAKNNNMKVNEADPLNWGVAAEAMKGSHLDEVKRMVEEYRKPVVRLGGETLTISQVAAIAAHDHGVQVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRYYS*