AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1123600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1123600 MtrunA17_Chr7g0264311 93.089 246 5 1 1 234 1 246 3.26e-161 463
Msa1123600 MtrunA17_Chr7g0264351 92.683 246 6 1 1 234 1 246 3.40e-161 463
Msa1123600 MtrunA17_Chr7g0264341 92.276 246 7 1 1 234 1 246 1.04e-159 459
Msa1123600 MtrunA17_Chr1g0181091 80.335 239 27 2 8 234 4 234 3.54e-129 381
Msa1123600 MtrunA17_Chr1g0199551 76.498 217 39 1 30 234 28 244 1.18e-115 346
Msa1123600 MtrunA17_Chr5g0448361 70.507 217 51 1 31 234 16 232 1.77e-101 309
Msa1123600 MtrunA17_Chr2g0326921 70.320 219 54 2 27 234 1 219 1.40e-100 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1123600 AT2G37040.1 76.522 230 42 1 17 234 18 247 5.27e-117 350
Msa1123600 AT3G53260.1 78.140 215 35 1 32 234 25 239 2.72e-113 340
Msa1123600 AT3G10340.1 75.111 225 43 2 23 234 6 230 1.01e-110 333
Msa1123600 AT5G04230.2 70.183 218 48 3 32 234 12 227 4.82e-98 301
Msa1123600 AT5G04230.1 70.183 218 48 3 32 234 12 227 5.05e-98 300

Find 69 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCCTCTGTCTTACATTGC+TGG 0.215263 7_3:+74558054 Msa1123600:CDS
CACACTGCTCCAAGGTTATT+CGG 0.232317 7_3:+74557930 Msa1123600:CDS
CTGCAAAGCACCACCTTGTT+TGG 0.301759 7_3:-74557079 Msa1123590:three_prime_UTR
GGGAATTAGATTTGAAATCT+TGG 0.372469 7_3:+74557951 Msa1123600:CDS
ATGGAGTGACGTTGTTGTTA+AGG 0.373628 7_3:-74557990 Msa1123590:three_prime_UTR
TACATTGCTGGATTGCTAAC+CGG 0.377877 7_3:+74558066 Msa1123600:CDS
GATCACAGCTTCAGGAGATT+TGG 0.382346 7_3:+74558032 Msa1123600:CDS
GAGTTAAGGCCAGCAGTGAC+TGG 0.386890 7_3:+74556980 Msa1123600:CDS
ATGAAGCTGATCCTTTGAAT+TGG 0.399923 7_3:+74556779 Msa1123600:CDS
TACGGTGTCACCACCGGGTT+CGG 0.400782 7_3:+74557036 Msa1123600:CDS
ATCTGCTAGGGCTGGAGTTA+AGG 0.404467 7_3:+74556966 Msa1123600:CDS
TGAAGCTGATCCTTTGAATT+GGG 0.415897 7_3:+74556780 Msa1123600:CDS
AATCAAGCAGAATGATTCAT+TGG 0.416820 7_3:-74556728 Msa1123590:three_prime_UTR
CGTGGTACGATCACAGCTTC+AGG 0.422509 7_3:+74558024 Msa1123600:CDS
CAAGACGGACCACCGGCTTC+CGG 0.424699 7_3:-74556867 Msa1123590:three_prime_UTR
AGCTTTAGAATTTGATCGTC+CGG 0.432653 7_3:-74558085 Msa1123590:intron
GTGCGAGGTGGCGCCGAACC+CGG 0.448163 7_3:-74557049 Msa1123590:three_prime_UTR
CTCCATCACCCAGTCACTGC+TGG 0.457530 7_3:-74556989 Msa1123590:three_prime_UTR
CTGTCTGAATCTGCTAGGGC+TGG 0.469696 7_3:+74556958 Msa1123600:CDS
TGGAGCTGTCTGAATCTGCT+AGG 0.480887 7_3:+74556953 Msa1123600:CDS
ACACTGCTCCAAGGTTATTC+GGG 0.483941 7_3:+74557931 Msa1123600:CDS
GACAGTTACGGTGTCACCAC+CGG 0.492715 7_3:+74557030 Msa1123600:CDS
AATGCTGGAATATTTGGAAA+CGG 0.493618 7_3:+74557844 Msa1123600:CDS
TCTAAAGCTCATGGACCATC+CGG 0.493757 7_3:+74558099 Msa1123600:CDS
ATCTAATTCCCGAATAACCT+TGG 0.497406 7_3:-74557939 Msa1123590:three_prime_UTR
ACTCCATGTTTACCACTTCG+TGG 0.503596 7_3:+74558006 Msa1123600:CDS
TGCACATGACCATGGTGTGC+AGG 0.506601 7_3:+74556930 Msa1123600:CDS
CTTTGCAGAAAGAACTCATA+AGG 0.510191 7_3:+74557094 Msa1123600:CDS
AGCGTATGGTGGAGGAGTAC+CGG 0.511195 7_3:+74556848 Msa1123600:CDS
CGATCAAATTCTAAAGCTCA+TGG 0.519333 7_3:+74558090 Msa1123600:CDS
GCATTAAGTATTTCTCCGGA+TGG 0.520440 7_3:-74558114 Msa1123590:intron
AACAAAGGCACGGACAGTTA+CGG 0.520670 7_3:+74557018 Msa1123600:CDS
GGCCAGCAGTGACTGGGTGA+TGG 0.539190 7_3:+74556987 Msa1123600:CDS
ACCATGGTCATGTGCAGCAA+TGG 0.541335 7_3:-74556923 Msa1123590:three_prime_UTR
GTTGTTAAGGAGCTTAGTGA+TGG 0.555678 7_3:-74557977 Msa1123590:three_prime_UTR
AAGCACCACCTTGTTTGGTA+CGG 0.564097 7_3:-74557074 Msa1123590:three_prime_UTR
GGTGGAGGAGTACCGGAAGC+CGG 0.566790 7_3:+74556855 Msa1123600:CDS
GGAGCTGTCTGAATCTGCTA+GGG 0.568528 7_3:+74556954 Msa1123600:CDS
GCTGCTCTTGTTGCCGTGTG+TGG 0.574124 7_3:-74557889 Msa1123590:three_prime_UTR
TTTGGTACGGCGGTGCGAGG+TGG 0.578567 7_3:-74557061 Msa1123590:three_prime_UTR
GCTGCCACACCCCAATTCAA+AGG 0.581598 7_3:-74556790 Msa1123590:three_prime_UTR
TCGCACCGCCGTACCAAACA+AGG 0.582783 7_3:+74557066 Msa1123600:CDS
TGAGGTGAAGCGTATGGTGG+AGG 0.584737 7_3:+74556840 Msa1123600:CDS
GCCATTGCTGCACATGACCA+TGG 0.585263 7_3:+74556922 Msa1123600:CDS
TGATCCTTTGAATTGGGGTG+TGG 0.587095 7_3:+74556786 Msa1123600:CDS
AGTTAAGGCCAGCAGTGACT+GGG 0.592895 7_3:+74556981 Msa1123600:CDS
GTGGCAGCTGAGGCAATGAA+AGG 0.593497 7_3:+74556805 Msa1123600:CDS
CACCACCTTGTTTGGTACGG+CGG 0.598168 7_3:-74557071 Msa1123590:three_prime_UTR
TTGTTTGGTACGGCGGTGCG+AGG 0.600854 7_3:-74557064 Msa1123590:three_prime_UTR
ACAGTTACGGTGTCACCACC+GGG 0.611533 7_3:+74557031 Msa1123600:CDS
GAATTGGGGTGTGGCAGCTG+AGG 0.617765 7_3:+74556795 Msa1123600:CDS
ATCCAGCAATGTAAGACAGA+GGG 0.626529 7_3:-74558056 Msa1123590:intron
CCTTGATGAGGTGAAGCGTA+TGG 0.628479 7_3:+74556834 Msa1123600:CDS
AAGTCACACACTACCACACA+CGG 0.628536 7_3:+74557876 Msa1123600:CDS
TGTGTTGATTCTCACTAGCA+TGG 0.632917 7_3:-74557911 Msa1123590:three_prime_UTR
AGAATCAACACACTGCTCCA+AGG 0.635657 7_3:+74557922 Msa1123600:CDS
GAAGCTGATCCTTTGAATTG+GGG 0.639983 7_3:+74556781 Msa1123600:CDS
GAAAGGCAGTCACCTTGATG+AGG 0.642522 7_3:+74556822 Msa1123600:CDS
GGAGGAGTACCGGAAGCCGG+TGG 0.645003 7_3:+74556858 Msa1123600:CDS
GTACCACGAAGTGGTAAACA+TGG 0.652497 7_3:-74558009 Msa1123590:intron
CCATACGCTTCACCTCATCA+AGG 0.653785 7_3:-74556834 Msa1123590:three_prime_UTR
GCTGTGATCGTACCACGAAG+TGG 0.655564 7_3:-74558018 Msa1123590:intron
TGATGAGGTGAAGCGTATGG+TGG 0.671300 7_3:+74556837 Msa1123600:CDS
GGAGAGTATGAACAAAGGCA+CGG 0.688439 7_3:+74557008 Msa1123600:CDS
ACAGCTCCACCTGCACACCA+TGG 0.693221 7_3:-74556939 Msa1123590:three_prime_UTR
ACATGACCATGGTGTGCAGG+TGG 0.694429 7_3:+74556933 Msa1123600:CDS
AATCCAGCAATGTAAGACAG+AGG 0.700859 7_3:-74558057 Msa1123590:intron
GTGATGGAGAGTATGAACAA+AGG 0.730510 7_3:+74557003 Msa1123600:CDS
CACCGCCGTACCAAACAAGG+TGG 0.735748 7_3:+74557069 Msa1123600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAAAATTTATTTAAAT+TGG + chr7_3:74557679-74557698 Msa1123600:intron 0.0%
!!! ATTTTAAAATTTATTTAAAT+TGG + chr7_3:74557679-74557698 Msa1123600:intron 0.0%
!!! CTTCTTTTAAAAAAAAAATA+TGG + chr7_3:74557719-74557738 Msa1123600:intron 10.0%
!!! CTTCTTTTAAAAAAAAAATA+TGG + chr7_3:74557719-74557738 Msa1123600:intron 10.0%
!!! AAAAATTATCCTTGTTATTT+TGG + chr7_3:74557271-74557290 Msa1123600:intron 15.0%
!!! AAAAATTATCCTTGTTATTT+TGG + chr7_3:74557271-74557290 Msa1123600:intron 15.0%
!! ATTAGAAATCCAAAATAACA+AGG - chr7_3:74557283-74557302 Msa1123590:intron 20.0%
!!! GTGTTTTTGTAACAATAAAA+AGG - chr7_3:74557527-74557546 Msa1123590:intron 20.0%
!! ATTAGAAATCCAAAATAACA+AGG - chr7_3:74557283-74557302 Msa1123590:intron 20.0%
!!! GTGTTTTTGTAACAATAAAA+AGG - chr7_3:74557527-74557546 Msa1123590:intron 20.0%
!!! CCAAGAAAAAAGTACTTATA+AGG - chr7_3:74557154-74557173 Msa1123590:intron 25.0%
!!! CCTTATAAGTACTTTTTTCT+TGG + chr7_3:74557151-74557170 Msa1123600:intron 25.0%
!!! TACTTTGAAATGAAAACAAG+TGG + chr7_3:74557406-74557425 Msa1123600:intron 25.0%
! ATTATTGTATTGAGAGATAG+TGG - chr7_3:74557634-74557653 Msa1123590:intron 25.0%
! CAATACAATAATACATGTGT+AGG + chr7_3:74557642-74557661 Msa1123600:intron 25.0%
!!! GCTACTTAATTCTTGTTTTT+AGG + chr7_3:74557815-74557834 Msa1123600:intron 25.0%
!! TTTTTGAATGCTGGAATATT+TGG + chr7_3:74557838-74557857 Msa1123600:CDS 25.0%
!! TTTTGCATTAAGTATTTCTC+CGG - chr7_3:74558121-74558140 Msa1123590:intron 25.0%
!!! CCAAGAAAAAAGTACTTATA+AGG - chr7_3:74557154-74557173 Msa1123590:intron 25.0%
!!! CCTTATAAGTACTTTTTTCT+TGG + chr7_3:74557151-74557170 Msa1123600:intron 25.0%
!!! TACTTTGAAATGAAAACAAG+TGG + chr7_3:74557406-74557425 Msa1123600:intron 25.0%
! ATTATTGTATTGAGAGATAG+TGG - chr7_3:74557634-74557653 Msa1123590:intron 25.0%
! CAATACAATAATACATGTGT+AGG + chr7_3:74557642-74557661 Msa1123600:intron 25.0%
!!! GCTACTTAATTCTTGTTTTT+AGG + chr7_3:74557815-74557834 Msa1123600:intron 25.0%
!! TTTTTGAATGCTGGAATATT+TGG + chr7_3:74557838-74557857 Msa1123600:CDS 25.0%
!! TTTTGCATTAAGTATTTCTC+CGG - chr7_3:74558121-74558140 Msa1123590:intron 25.0%
AATCAAGCAGAATGATTCAT+TGG - chr7_3:74556731-74556750 Msa1123590:three_prime_UTR 30.0%
TTGAAATGAAAACAAGTGGT+AGG + chr7_3:74557410-74557429 Msa1123600:intron 30.0%
TGAAATGAAAACAAGTGGTA+GGG + chr7_3:74557411-74557430 Msa1123600:intron 30.0%
AGTTAAACACTGTAGAAAAC+TGG - chr7_3:74557479-74557498 Msa1123590:intron 30.0%
TCTCTCACCTATTTAATAGA+GGG + chr7_3:74557547-74557566 Msa1123600:intron 30.0%
CCATTAACCCTCTATTAAAT+AGG - chr7_3:74557557-74557576 Msa1123590:intron 30.0%
CCTATTTAATAGAGGGTTAA+TGG + chr7_3:74557554-74557573 Msa1123600:intron 30.0%
ATTTAATAGAGGGTTAATGG+AGG + chr7_3:74557557-74557576 Msa1123600:intron 30.0%
TTTAATAGAGGGTTAATGGA+GGG + chr7_3:74557558-74557577 Msa1123600:intron 30.0%
TACACATGAGAGAAAAAAAC+TGG + chr7_3:74557588-74557607 Msa1123600:intron 30.0%
!!! GTTTTTAGGTTTTTGAATGC+TGG + chr7_3:74557829-74557848 Msa1123600:intron 30.0%
AATGCTGGAATATTTGGAAA+CGG + chr7_3:74557844-74557863 Msa1123600:CDS 30.0%
! GGGAATTAGATTTGAAATCT+TGG + chr7_3:74557951-74557970 Msa1123600:CDS 30.0%
AATCAAGCAGAATGATTCAT+TGG - chr7_3:74556731-74556750 Msa1123590:three_prime_UTR 30.0%
TTGAAATGAAAACAAGTGGT+AGG + chr7_3:74557410-74557429 Msa1123600:intron 30.0%
TGAAATGAAAACAAGTGGTA+GGG + chr7_3:74557411-74557430 Msa1123600:intron 30.0%
AGTTAAACACTGTAGAAAAC+TGG - chr7_3:74557479-74557498 Msa1123590:intron 30.0%
TCTCTCACCTATTTAATAGA+GGG + chr7_3:74557547-74557566 Msa1123600:intron 30.0%
CCATTAACCCTCTATTAAAT+AGG - chr7_3:74557557-74557576 Msa1123590:intron 30.0%
CCTATTTAATAGAGGGTTAA+TGG + chr7_3:74557554-74557573 Msa1123600:intron 30.0%
ATTTAATAGAGGGTTAATGG+AGG + chr7_3:74557557-74557576 Msa1123600:intron 30.0%
TTTAATAGAGGGTTAATGGA+GGG + chr7_3:74557558-74557577 Msa1123600:intron 30.0%
TACACATGAGAGAAAAAAAC+TGG + chr7_3:74557588-74557607 Msa1123600:intron 30.0%
!!! GTTTTTAGGTTTTTGAATGC+TGG + chr7_3:74557829-74557848 Msa1123600:intron 30.0%
AATGCTGGAATATTTGGAAA+CGG + chr7_3:74557844-74557863 Msa1123600:CDS 30.0%
! GGGAATTAGATTTGAAATCT+TGG + chr7_3:74557951-74557970 Msa1123600:CDS 30.0%
ATGAAGCTGATCCTTTGAAT+TGG + chr7_3:74556779-74556798 Msa1123600:CDS 35.0%
TGAAGCTGATCCTTTGAATT+GGG + chr7_3:74556780-74556799 Msa1123600:CDS 35.0%
CTTTGCAGAAAGAACTCATA+AGG + chr7_3:74557094-74557113 Msa1123600:CDS 35.0%
TAAACACTGTAGAAAACTGG+AGG - chr7_3:74557476-74557495 Msa1123590:intron 35.0%
! AGAGAGAGTATAATCAAAGG+TGG - chr7_3:74557504-74557523 Msa1123590:intron 35.0%
! AGGAGAGAGAGTATAATCAA+AGG - chr7_3:74557507-74557526 Msa1123590:intron 35.0%
CTCTCTCACCTATTTAATAG+AGG + chr7_3:74557546-74557565 Msa1123600:intron 35.0%
! TATTGAGAGATAGTGGTTAG+CGG - chr7_3:74557627-74557646 Msa1123590:intron 35.0%
ATCTAATTCCCGAATAACCT+TGG - chr7_3:74557942-74557961 Msa1123590:three_prime_UTR 35.0%
!! AGCTTTAGAATTTGATCGTC+CGG - chr7_3:74558088-74558107 Msa1123590:intron 35.0%
!! CGATCAAATTCTAAAGCTCA+TGG + chr7_3:74558090-74558109 Msa1123600:CDS 35.0%
! CAAAAGAAGCTTTTATCTGC+TGG + chr7_3:74558136-74558155 Msa1123600:CDS 35.0%
ATGAAGCTGATCCTTTGAAT+TGG + chr7_3:74556779-74556798 Msa1123600:CDS 35.0%
TGAAGCTGATCCTTTGAATT+GGG + chr7_3:74556780-74556799 Msa1123600:CDS 35.0%
CTTTGCAGAAAGAACTCATA+AGG + chr7_3:74557094-74557113 Msa1123600:CDS 35.0%
TAAACACTGTAGAAAACTGG+AGG - chr7_3:74557476-74557495 Msa1123590:intron 35.0%
! AGAGAGAGTATAATCAAAGG+TGG - chr7_3:74557504-74557523 Msa1123590:intron 35.0%
! AGGAGAGAGAGTATAATCAA+AGG - chr7_3:74557507-74557526 Msa1123590:intron 35.0%
CTCTCTCACCTATTTAATAG+AGG + chr7_3:74557546-74557565 Msa1123600:intron 35.0%
! TATTGAGAGATAGTGGTTAG+CGG - chr7_3:74557627-74557646 Msa1123590:intron 35.0%
ATCTAATTCCCGAATAACCT+TGG - chr7_3:74557942-74557961 Msa1123590:three_prime_UTR 35.0%
!! AGCTTTAGAATTTGATCGTC+CGG - chr7_3:74558088-74558107 Msa1123590:intron 35.0%
!! CGATCAAATTCTAAAGCTCA+TGG + chr7_3:74558090-74558109 Msa1123600:CDS 35.0%
! CAAAAGAAGCTTTTATCTGC+TGG + chr7_3:74558136-74558155 Msa1123600:CDS 35.0%
GAAGCTGATCCTTTGAATTG+GGG + chr7_3:74556781-74556800 Msa1123600:CDS 40.0%
! GTGATGGAGAGTATGAACAA+AGG + chr7_3:74557003-74557022 Msa1123600:CDS 40.0%
! ATTGAGAGATAGTGGTTAGC+GGG - chr7_3:74557626-74557645 Msa1123590:intron 40.0%
!! TGTGTTGATTCTCACTAGCA+TGG - chr7_3:74557914-74557933 Msa1123590:three_prime_UTR 40.0%
GTTGTTAAGGAGCTTAGTGA+TGG - chr7_3:74557980-74557999 Msa1123590:three_prime_UTR 40.0%
! ATGGAGTGACGTTGTTGTTA+AGG - chr7_3:74557993-74558012 Msa1123590:three_prime_UTR 40.0%
ATCCAGCAATGTAAGACAGA+GGG - chr7_3:74558059-74558078 Msa1123590:intron 40.0%
AATCCAGCAATGTAAGACAG+AGG - chr7_3:74558060-74558079 Msa1123590:intron 40.0%
TACATTGCTGGATTGCTAAC+CGG + chr7_3:74558066-74558085 Msa1123600:CDS 40.0%
GCATTAAGTATTTCTCCGGA+TGG - chr7_3:74558117-74558136 Msa1123590:intron 40.0%
GAAGCTGATCCTTTGAATTG+GGG + chr7_3:74556781-74556800 Msa1123600:CDS 40.0%
! GTGATGGAGAGTATGAACAA+AGG + chr7_3:74557003-74557022 Msa1123600:CDS 40.0%
! ATTGAGAGATAGTGGTTAGC+GGG - chr7_3:74557626-74557645 Msa1123590:intron 40.0%
!! TGTGTTGATTCTCACTAGCA+TGG - chr7_3:74557914-74557933 Msa1123590:three_prime_UTR 40.0%
GTTGTTAAGGAGCTTAGTGA+TGG - chr7_3:74557980-74557999 Msa1123590:three_prime_UTR 40.0%
! ATGGAGTGACGTTGTTGTTA+AGG - chr7_3:74557993-74558012 Msa1123590:three_prime_UTR 40.0%
ATCCAGCAATGTAAGACAGA+GGG - chr7_3:74558059-74558078 Msa1123590:intron 40.0%
AATCCAGCAATGTAAGACAG+AGG - chr7_3:74558060-74558079 Msa1123590:intron 40.0%
TACATTGCTGGATTGCTAAC+CGG + chr7_3:74558066-74558085 Msa1123600:CDS 40.0%
GCATTAAGTATTTCTCCGGA+TGG - chr7_3:74558117-74558136 Msa1123590:intron 40.0%
TGATCCTTTGAATTGGGGTG+TGG + chr7_3:74556786-74556805 Msa1123600:CDS 45.0%
! GGAGAGTATGAACAAAGGCA+CGG + chr7_3:74557008-74557027 Msa1123600:CDS 45.0%
! AACAAAGGCACGGACAGTTA+CGG + chr7_3:74557018-74557037 Msa1123600:CDS 45.0%
! AAGCACCACCTTGTTTGGTA+CGG - chr7_3:74557077-74557096 Msa1123590:three_prime_UTR 45.0%
!! AAAACTGGAGGTATCTCCAC+TGG - chr7_3:74557464-74557483 Msa1123590:intron 45.0%
AAGTCACACACTACCACACA+CGG + chr7_3:74557876-74557895 Msa1123600:CDS 45.0%
AGAATCAACACACTGCTCCA+AGG + chr7_3:74557922-74557941 Msa1123600:CDS 45.0%
CACACTGCTCCAAGGTTATT+CGG + chr7_3:74557930-74557949 Msa1123600:CDS 45.0%
ACACTGCTCCAAGGTTATTC+GGG + chr7_3:74557931-74557950 Msa1123600:CDS 45.0%
ACTCCATGTTTACCACTTCG+TGG + chr7_3:74558006-74558025 Msa1123600:CDS 45.0%
GTACCACGAAGTGGTAAACA+TGG - chr7_3:74558012-74558031 Msa1123590:intron 45.0%
GATCACAGCTTCAGGAGATT+TGG + chr7_3:74558032-74558051 Msa1123600:CDS 45.0%
! TCTAAAGCTCATGGACCATC+CGG + chr7_3:74558099-74558118 Msa1123600:CDS 45.0%
TGATCCTTTGAATTGGGGTG+TGG + chr7_3:74556786-74556805 Msa1123600:CDS 45.0%
! GGAGAGTATGAACAAAGGCA+CGG + chr7_3:74557008-74557027 Msa1123600:CDS 45.0%
! AACAAAGGCACGGACAGTTA+CGG + chr7_3:74557018-74557037 Msa1123600:CDS 45.0%
! AAGCACCACCTTGTTTGGTA+CGG - chr7_3:74557077-74557096 Msa1123590:three_prime_UTR 45.0%
!! AAAACTGGAGGTATCTCCAC+TGG - chr7_3:74557464-74557483 Msa1123590:intron 45.0%
AAGTCACACACTACCACACA+CGG + chr7_3:74557876-74557895 Msa1123600:CDS 45.0%
AGAATCAACACACTGCTCCA+AGG + chr7_3:74557922-74557941 Msa1123600:CDS 45.0%
CACACTGCTCCAAGGTTATT+CGG + chr7_3:74557930-74557949 Msa1123600:CDS 45.0%
ACACTGCTCCAAGGTTATTC+GGG + chr7_3:74557931-74557950 Msa1123600:CDS 45.0%
ACTCCATGTTTACCACTTCG+TGG + chr7_3:74558006-74558025 Msa1123600:CDS 45.0%
GTACCACGAAGTGGTAAACA+TGG - chr7_3:74558012-74558031 Msa1123590:intron 45.0%
GATCACAGCTTCAGGAGATT+TGG + chr7_3:74558032-74558051 Msa1123600:CDS 45.0%
! TCTAAAGCTCATGGACCATC+CGG + chr7_3:74558099-74558118 Msa1123600:CDS 45.0%
!! ATTCTTAAATAAAAAAATAT+GGG - chr7_3:74557311-74557330 Msa1123590:intron 5.0%
!! TATTCTTAAATAAAAAAATA+TGG - chr7_3:74557312-74557331 Msa1123590:intron 5.0%
!!! TAATATTTTAAATCAAATAA+AGG + chr7_3:74557343-74557362 Msa1123600:intron 5.0%
!! ATTCTTAAATAAAAAAATAT+GGG - chr7_3:74557311-74557330 Msa1123590:intron 5.0%
!! TATTCTTAAATAAAAAAATA+TGG - chr7_3:74557312-74557331 Msa1123590:intron 5.0%
!!! TAATATTTTAAATCAAATAA+AGG + chr7_3:74557343-74557362 Msa1123600:intron 5.0%
GAAAGGCAGTCACCTTGATG+AGG + chr7_3:74556822-74556841 Msa1123600:CDS 50.0%
CCATACGCTTCACCTCATCA+AGG - chr7_3:74556837-74556856 Msa1123590:three_prime_UTR 50.0%
CCTTGATGAGGTGAAGCGTA+TGG + chr7_3:74556834-74556853 Msa1123600:CDS 50.0%
TGATGAGGTGAAGCGTATGG+TGG + chr7_3:74556837-74556856 Msa1123600:CDS 50.0%
CGAGACACTGACGATTTCTC+AGG + chr7_3:74556894-74556913 Msa1123600:intron 50.0%
GACACTGACGATTTCTCAGG+TGG + chr7_3:74556897-74556916 Msa1123600:intron 50.0%
ACCATGGTCATGTGCAGCAA+TGG - chr7_3:74556926-74556945 Msa1123590:three_prime_UTR 50.0%
TGGAGCTGTCTGAATCTGCT+AGG + chr7_3:74556953-74556972 Msa1123600:CDS 50.0%
GGAGCTGTCTGAATCTGCTA+GGG + chr7_3:74556954-74556973 Msa1123600:CDS 50.0%
ATCTGCTAGGGCTGGAGTTA+AGG + chr7_3:74556966-74556985 Msa1123600:CDS 50.0%
AGTTAAGGCCAGCAGTGACT+GGG + chr7_3:74556981-74557000 Msa1123600:CDS 50.0%
! CTGCAAAGCACCACCTTGTT+TGG - chr7_3:74557082-74557101 Msa1123590:three_prime_UTR 50.0%
GTGGTAGGGTATGCTAACCA+CGG + chr7_3:74557425-74557444 Msa1123600:intron 50.0%
TGGTAGGGTATGCTAACCAC+GGG + chr7_3:74557426-74557445 Msa1123600:intron 50.0%
GTCCCTCTGTCTTACATTGC+TGG + chr7_3:74558054-74558073 Msa1123600:CDS 50.0%
GAAAGGCAGTCACCTTGATG+AGG + chr7_3:74556822-74556841 Msa1123600:CDS 50.0%
CCATACGCTTCACCTCATCA+AGG - chr7_3:74556837-74556856 Msa1123590:three_prime_UTR 50.0%
CCTTGATGAGGTGAAGCGTA+TGG + chr7_3:74556834-74556853 Msa1123600:CDS 50.0%
TGATGAGGTGAAGCGTATGG+TGG + chr7_3:74556837-74556856 Msa1123600:CDS 50.0%
CGAGACACTGACGATTTCTC+AGG + chr7_3:74556894-74556913 Msa1123600:intron 50.0%
GACACTGACGATTTCTCAGG+TGG + chr7_3:74556897-74556916 Msa1123600:intron 50.0%
ACCATGGTCATGTGCAGCAA+TGG - chr7_3:74556926-74556945 Msa1123590:three_prime_UTR 50.0%
TGGAGCTGTCTGAATCTGCT+AGG + chr7_3:74556953-74556972 Msa1123600:CDS 50.0%
GGAGCTGTCTGAATCTGCTA+GGG + chr7_3:74556954-74556973 Msa1123600:CDS 50.0%
ATCTGCTAGGGCTGGAGTTA+AGG + chr7_3:74556966-74556985 Msa1123600:CDS 50.0%
AGTTAAGGCCAGCAGTGACT+GGG + chr7_3:74556981-74557000 Msa1123600:CDS 50.0%
! CTGCAAAGCACCACCTTGTT+TGG - chr7_3:74557082-74557101 Msa1123590:three_prime_UTR 50.0%
GTGGTAGGGTATGCTAACCA+CGG + chr7_3:74557425-74557444 Msa1123600:intron 50.0%
TGGTAGGGTATGCTAACCAC+GGG + chr7_3:74557426-74557445 Msa1123600:intron 50.0%
GTCCCTCTGTCTTACATTGC+TGG + chr7_3:74558054-74558073 Msa1123600:CDS 50.0%
GCTGCCACACCCCAATTCAA+AGG - chr7_3:74556793-74556812 Msa1123590:three_prime_UTR 55.0%
GTGGCAGCTGAGGCAATGAA+AGG + chr7_3:74556805-74556824 Msa1123600:CDS 55.0%
TGAGGTGAAGCGTATGGTGG+AGG + chr7_3:74556840-74556859 Msa1123600:CDS 55.0%
! AGCGTATGGTGGAGGAGTAC+CGG + chr7_3:74556848-74556867 Msa1123600:CDS 55.0%
TCAGTGTCTCGCCACCAAGA+CGG - chr7_3:74556885-74556904 Msa1123590:three_prime_UTR 55.0%
GCCATTGCTGCACATGACCA+TGG + chr7_3:74556922-74556941 Msa1123600:CDS 55.0%
TGCACATGACCATGGTGTGC+AGG + chr7_3:74556930-74556949 Msa1123600:CDS 55.0%
ACATGACCATGGTGTGCAGG+TGG + chr7_3:74556933-74556952 Msa1123600:CDS 55.0%
CTGTCTGAATCTGCTAGGGC+TGG + chr7_3:74556958-74556977 Msa1123600:CDS 55.0%
GAGTTAAGGCCAGCAGTGAC+TGG + chr7_3:74556980-74556999 Msa1123600:CDS 55.0%
!! GACAGTTACGGTGTCACCAC+CGG + chr7_3:74557030-74557049 Msa1123600:CDS 55.0%
!! ACAGTTACGGTGTCACCACC+GGG + chr7_3:74557031-74557050 Msa1123600:CDS 55.0%
! CACCACCTTGTTTGGTACGG+CGG - chr7_3:74557074-74557093 Msa1123590:three_prime_UTR 55.0%
!! TGGAGGTATCTCCACTGGAG+AGG - chr7_3:74557459-74557478 Msa1123590:intron 55.0%
! GAGAGATAGTGGTTAGCGGG+AGG - chr7_3:74557623-74557642 Msa1123590:intron 55.0%
GCTGTGATCGTACCACGAAG+TGG - chr7_3:74558021-74558040 Msa1123590:intron 55.0%
CGTGGTACGATCACAGCTTC+AGG + chr7_3:74558024-74558043 Msa1123600:CDS 55.0%
GCTGCCACACCCCAATTCAA+AGG - chr7_3:74556793-74556812 Msa1123590:three_prime_UTR 55.0%
GTGGCAGCTGAGGCAATGAA+AGG + chr7_3:74556805-74556824 Msa1123600:CDS 55.0%
TGAGGTGAAGCGTATGGTGG+AGG + chr7_3:74556840-74556859 Msa1123600:CDS 55.0%
! AGCGTATGGTGGAGGAGTAC+CGG + chr7_3:74556848-74556867 Msa1123600:CDS 55.0%
TCAGTGTCTCGCCACCAAGA+CGG - chr7_3:74556885-74556904 Msa1123590:three_prime_UTR 55.0%
GCCATTGCTGCACATGACCA+TGG + chr7_3:74556922-74556941 Msa1123600:CDS 55.0%
TGCACATGACCATGGTGTGC+AGG + chr7_3:74556930-74556949 Msa1123600:CDS 55.0%
ACATGACCATGGTGTGCAGG+TGG + chr7_3:74556933-74556952 Msa1123600:CDS 55.0%
CTGTCTGAATCTGCTAGGGC+TGG + chr7_3:74556958-74556977 Msa1123600:CDS 55.0%
GAGTTAAGGCCAGCAGTGAC+TGG + chr7_3:74556980-74556999 Msa1123600:CDS 55.0%
!! GACAGTTACGGTGTCACCAC+CGG + chr7_3:74557030-74557049 Msa1123600:CDS 55.0%
!! ACAGTTACGGTGTCACCACC+GGG + chr7_3:74557031-74557050 Msa1123600:CDS 55.0%
! CACCACCTTGTTTGGTACGG+CGG - chr7_3:74557074-74557093 Msa1123590:three_prime_UTR 55.0%
!! TGGAGGTATCTCCACTGGAG+AGG - chr7_3:74557459-74557478 Msa1123590:intron 55.0%
! GAGAGATAGTGGTTAGCGGG+AGG - chr7_3:74557623-74557642 Msa1123590:intron 55.0%
GCTGTGATCGTACCACGAAG+TGG - chr7_3:74558021-74558040 Msa1123590:intron 55.0%
CGTGGTACGATCACAGCTTC+AGG + chr7_3:74558024-74558043 Msa1123600:CDS 55.0%
GAATTGGGGTGTGGCAGCTG+AGG + chr7_3:74556795-74556814 Msa1123600:CDS 60.0%
ACAGCTCCACCTGCACACCA+TGG - chr7_3:74556942-74556961 Msa1123590:three_prime_UTR 60.0%
CTCCATCACCCAGTCACTGC+TGG - chr7_3:74556992-74557011 Msa1123590:three_prime_UTR 60.0%
!! TACGGTGTCACCACCGGGTT+CGG + chr7_3:74557036-74557055 Msa1123600:CDS 60.0%
TTGTTTGGTACGGCGGTGCG+AGG - chr7_3:74557067-74557086 Msa1123590:three_prime_UTR 60.0%
TCGCACCGCCGTACCAAACA+AGG + chr7_3:74557066-74557085 Msa1123600:CDS 60.0%
CACCGCCGTACCAAACAAGG+TGG + chr7_3:74557069-74557088 Msa1123600:CDS 60.0%
GGGTATGCTAACCACGGGAG+TGG + chr7_3:74557431-74557450 Msa1123600:intron 60.0%
GCTGCTCTTGTTGCCGTGTG+TGG - chr7_3:74557892-74557911 Msa1123590:three_prime_UTR 60.0%
GAATTGGGGTGTGGCAGCTG+AGG + chr7_3:74556795-74556814 Msa1123600:CDS 60.0%
ACAGCTCCACCTGCACACCA+TGG - chr7_3:74556942-74556961 Msa1123590:three_prime_UTR 60.0%
CTCCATCACCCAGTCACTGC+TGG - chr7_3:74556992-74557011 Msa1123590:three_prime_UTR 60.0%
!! TACGGTGTCACCACCGGGTT+CGG + chr7_3:74557036-74557055 Msa1123600:CDS 60.0%
TTGTTTGGTACGGCGGTGCG+AGG - chr7_3:74557067-74557086 Msa1123590:three_prime_UTR 60.0%
TCGCACCGCCGTACCAAACA+AGG + chr7_3:74557066-74557085 Msa1123600:CDS 60.0%
CACCGCCGTACCAAACAAGG+TGG + chr7_3:74557069-74557088 Msa1123600:CDS 60.0%
GGGTATGCTAACCACGGGAG+TGG + chr7_3:74557431-74557450 Msa1123600:intron 60.0%
GCTGCTCTTGTTGCCGTGTG+TGG - chr7_3:74557892-74557911 Msa1123590:three_prime_UTR 60.0%
!! GGTGGAGGAGTACCGGAAGC+CGG + chr7_3:74556855-74556874 Msa1123600:CDS 65.0%
!! CAAGACGGACCACCGGCTTC+CGG - chr7_3:74556870-74556889 Msa1123590:three_prime_UTR 65.0%
AAGCCGGTGGTCCGTCTTGG+TGG + chr7_3:74556871-74556890 Msa1123600:intron 65.0%
TCGCCACCAAGACGGACCAC+CGG - chr7_3:74556877-74556896 Msa1123590:three_prime_UTR 65.0%
GGCCAGCAGTGACTGGGTGA+TGG + chr7_3:74556987-74557006 Msa1123600:CDS 65.0%
TTTGGTACGGCGGTGCGAGG+TGG - chr7_3:74557064-74557083 Msa1123590:three_prime_UTR 65.0%
CTGGAGAGGATCCACTCCCG+TGG - chr7_3:74557445-74557464 Msa1123590:intron 65.0%
CGGGAGTGGATCCTCTCCAG+TGG + chr7_3:74557445-74557464 Msa1123600:intron 65.0%
!! GGTGGAGGAGTACCGGAAGC+CGG + chr7_3:74556855-74556874 Msa1123600:CDS 65.0%
!! CAAGACGGACCACCGGCTTC+CGG - chr7_3:74556870-74556889 Msa1123590:three_prime_UTR 65.0%
AAGCCGGTGGTCCGTCTTGG+TGG + chr7_3:74556871-74556890 Msa1123600:intron 65.0%
TCGCCACCAAGACGGACCAC+CGG - chr7_3:74556877-74556896 Msa1123590:three_prime_UTR 65.0%
GGCCAGCAGTGACTGGGTGA+TGG + chr7_3:74556987-74557006 Msa1123600:CDS 65.0%
TTTGGTACGGCGGTGCGAGG+TGG - chr7_3:74557064-74557083 Msa1123590:three_prime_UTR 65.0%
CTGGAGAGGATCCACTCCCG+TGG - chr7_3:74557445-74557464 Msa1123590:intron 65.0%
CGGGAGTGGATCCTCTCCAG+TGG + chr7_3:74557445-74557464 Msa1123600:intron 65.0%
!! GGAGGAGTACCGGAAGCCGG+TGG + chr7_3:74556858-74556877 Msa1123600:CDS 70.0%
CGGAAGCCGGTGGTCCGTCT+TGG + chr7_3:74556868-74556887 Msa1123600:intron 70.0%
!! GGAGGAGTACCGGAAGCCGG+TGG + chr7_3:74556858-74556877 Msa1123600:CDS 70.0%
CGGAAGCCGGTGGTCCGTCT+TGG + chr7_3:74556868-74556887 Msa1123600:intron 70.0%
! GTGCGAGGTGGCGCCGAACC+CGG - chr7_3:74557052-74557071 Msa1123590:three_prime_UTR 75.0%
! GTGCGAGGTGGCGCCGAACC+CGG - chr7_3:74557052-74557071 Msa1123590:three_prime_UTR 75.0%
!! CGAGGTGGCGCCGAACCCGG+TGG - chr7_3:74557049-74557068 Msa1123590:three_prime_UTR 80.0%
!! CGAGGTGGCGCCGAACCCGG+TGG - chr7_3:74557049-74557068 Msa1123590:three_prime_UTR 80.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 74556691 74558170 74556691 ID=Msa1123600;Name=Msa1123600
chr7_3 mRNA 74556691 74558170 74556691 ID=Msa1123600-mRNA-1;Parent=Msa1123600;Name=Msa1123600-mRNA-1;_AED=0.42;_eAED=0.44;_QI=0|0|0|1|0.5|0.33|3|0|240
chr7_3 exon 74556691 74556879 74556691 ID=Msa1123600-mRNA-1:exon:19827;Parent=Msa1123600-mRNA-1
chr7_3 exon 74556916 74557115 74556916 ID=Msa1123600-mRNA-1:exon:19828;Parent=Msa1123600-mRNA-1
chr7_3 exon 74557837 74558170 74557837 ID=Msa1123600-mRNA-1:exon:19829;Parent=Msa1123600-mRNA-1
chr7_3 CDS 74556691 74556879 74556691 ID=Msa1123600-mRNA-1:cds;Parent=Msa1123600-mRNA-1
chr7_3 CDS 74556916 74557115 74556916 ID=Msa1123600-mRNA-1:cds;Parent=Msa1123600-mRNA-1
chr7_3 CDS 74557837 74558170 74557837 ID=Msa1123600-mRNA-1:cds;Parent=Msa1123600-mRNA-1
Gene Sequence

>Msa1123600

ATGGAAGCAATATCAGCAGCTATCACAAAAAACAATGCCAATGAATCATTCTGCTTGATTCATGCAAAGAATAATAACATGAAAGTGAATGAAGCTGATCCTTTGAATTGGGGTGTGGCAGCTGAGGCAATGAAAGGCAGTCACCTTGATGAGGTGAAGCGTATGGTGGAGGAGTACCGGAAGCCGGTGGTGGCTGCCATTGCTGCACATGACCATGGTGTGCAGGTGGAGCTGTCTGAATCTGCTAGGGCTGGAGTTAAGGCCAGCAGTGACTGGGTGATGGAGAGTATGAACAAAGGCACGGACAGTTACGGTGTCACCACCGGGTTCGGCGCCACCTCGCACCGCCGTACCAAACAAGGTGGTGCTTTGCAGAAAGAACTCATAAGGTTTTTGAATGCTGGAATATTTGGAAACGGAACTGAGTCAAGTCACACACTACCACACACGGCAACAAGAGCAGCCATGCTAGTGAGAATCAACACACTGCTCCAAGGTTATTCGGGAATTAGATTTGAAATCTTGGAAGCCATCACTAAGCTCCTTAACAACAACGTCACTCCATGTTTACCACTTCGTGGTACGATCACAGCTTCAGGAGATTTGGTCCCTCTGTCTTACATTGCTGGATTGCTAACCGGACGATCAAATTCTAAAGCTCATGGACCATCCGGAGAAATACTTAATGCAAAAGAAGCTTTTATCTGCTGGAATCAATGCTGA
Protein sequence

>Msa1123600

MEAISAAITKNNANESFCLIHAKNNNMKVNEADPLNWGVAAEAMKGSHLDEVKRMVEEYRKPVVAAIAAHDHGVQVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNGTESSHTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNVTPCLPLRGTITASGDLVPLSYIAGLLTGRSNSKAHGPSGEILNAKEAFICWNQC*